LOCUS       DAZBTH010000001        82976 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000001 DAZBTH010000000
VERSION     DAZBTH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.001"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..488
                     /locus_tag="ACHGNW_000001"
     CDS             <1..488
                     /locus_tag="ACHGNW_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6924361.1"
                     /translation="SITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIY
                     VRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKR
                     NIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKF
                     L"
     gene            616..1299
                     /locus_tag="ACHGNW_000002"
     CDS             616..1299
                     /locus_tag="ACHGNW_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN6924362.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1608..1681
                     /locus_tag="ACHGNW_000003"
     tRNA            1608..1681
                     /locus_tag="ACHGNW_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Arg,seq:tct)
     gene            1840..2199
                     /locus_tag="ACHGNW_000004"
     CDS             1840..2199
                     /locus_tag="ACHGNW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN6924363.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2322..3953
                     /locus_tag="ACHGNW_000005"
     CDS             2322..3953
                     /locus_tag="ACHGNW_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN6924364.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4015..4656)
                     /locus_tag="ACHGNW_000006"
     CDS             complement(4015..4656)
                     /locus_tag="ACHGNW_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6924365.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4842..4918
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4938..5246
                     /gene="rplU"
                     /locus_tag="ACHGNW_000007"
     CDS             4938..5246
                     /gene="rplU"
                     /locus_tag="ACHGNW_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN6924366.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5273..5617
                     /locus_tag="ACHGNW_000008"
     CDS             5273..5617
                     /locus_tag="ACHGNW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN6924367.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5630..5923
                     /gene="rpmA"
                     /locus_tag="ACHGNW_000009"
     CDS             5630..5923
                     /gene="rpmA"
                     /locus_tag="ACHGNW_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN6924368.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6357..9164
                     /locus_tag="ACHGNW_000010"
     CDS             6357..9164
                     /locus_tag="ACHGNW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6924369.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9355..9846
                     /locus_tag="ACHGNW_000011"
     CDS             9355..9846
                     /locus_tag="ACHGNW_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6924370.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9949..10938
                     /locus_tag="ACHGNW_000012"
     CDS             9949..10938
                     /locus_tag="ACHGNW_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6924371.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10971..11774
                     /locus_tag="ACHGNW_000013"
     CDS             10971..11774
                     /locus_tag="ACHGNW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN6924372.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11795..12709
                     /locus_tag="ACHGNW_000014"
     CDS             11795..12709
                     /locus_tag="ACHGNW_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6924373.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12792..13166
                     /locus_tag="ACHGNW_000015"
     CDS             12792..13166
                     /locus_tag="ACHGNW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN6924374.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13305..13550
                     /locus_tag="ACHGNW_000016"
     CDS             13305..13550
                     /locus_tag="ACHGNW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6924375.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13722..14786
                     /locus_tag="ACHGNW_000017"
     CDS             13722..14786
                     /locus_tag="ACHGNW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN6924376.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15032..15457
                     /locus_tag="ACHGNW_000018"
     CDS             15032..15457
                     /locus_tag="ACHGNW_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6924377.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15450..15899
                     /gene="nusB"
                     /locus_tag="ACHGNW_000019"
     CDS             15450..15899
                     /gene="nusB"
                     /locus_tag="ACHGNW_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN6924378.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15996..16850
                     /locus_tag="ACHGNW_000020"
     CDS             15996..16850
                     /locus_tag="ACHGNW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN6924379.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16851..18188
                     /gene="xseA"
                     /locus_tag="ACHGNW_000021"
     CDS             16851..18188
                     /gene="xseA"
                     /locus_tag="ACHGNW_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN6924380.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18193..18417
                     /locus_tag="ACHGNW_000022"
     CDS             18193..18417
                     /locus_tag="ACHGNW_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN6924381.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18419..19309
                     /locus_tag="ACHGNW_000023"
     CDS             18419..19309
                     /locus_tag="ACHGNW_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6924382.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19318..20136
                     /locus_tag="ACHGNW_000024"
     CDS             19318..20136
                     /locus_tag="ACHGNW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN6924383.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20243..20692
                     /locus_tag="ACHGNW_000025"
     CDS             20243..20692
                     /locus_tag="ACHGNW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6924384.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20710..22392
                     /gene="recN"
                     /locus_tag="ACHGNW_000026"
     CDS             20710..22392
                     /gene="recN"
                     /locus_tag="ACHGNW_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN6924385.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22447..22563)
                     /locus_tag="ACHGNW_000027"
     CDS             complement(22447..22563)
                     /locus_tag="ACHGNW_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN6924386.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22636..23592)
                     /locus_tag="ACHGNW_000028"
     CDS             complement(22636..23592)
                     /locus_tag="ACHGNW_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6924387.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23763..24131
                     /locus_tag="ACHGNW_000029"
     CDS             23763..24131
                     /locus_tag="ACHGNW_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN6924388.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24301..24732
                     /gene="mraZ"
                     /locus_tag="ACHGNW_000030"
     CDS             24301..24732
                     /gene="mraZ"
                     /locus_tag="ACHGNW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN6924389.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24753..25712
                     /gene="rsmH"
                     /locus_tag="ACHGNW_000031"
     CDS             24753..25712
                     /gene="rsmH"
                     /locus_tag="ACHGNW_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN6924390.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25717..26118
                     /gene="ftsL"
                     /locus_tag="ACHGNW_000032"
     CDS             25717..26118
                     /gene="ftsL"
                     /locus_tag="ACHGNW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN6924391.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26119..28311
                     /locus_tag="ACHGNW_000033"
     CDS             26119..28311
                     /locus_tag="ACHGNW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6924392.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28337..29299
                     /gene="mraY"
                     /locus_tag="ACHGNW_000034"
     CDS             28337..29299
                     /gene="mraY"
                     /locus_tag="ACHGNW_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN6924393.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29313..30674
                     /gene="murD"
                     /locus_tag="ACHGNW_000035"
     CDS             29313..30674
                     /gene="murD"
                     /locus_tag="ACHGNW_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN6924394.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30700..31788
                     /gene="murG"
                     /locus_tag="ACHGNW_000036"
     CDS             30700..31788
                     /gene="murG"
                     /locus_tag="ACHGNW_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6924395.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31803..33023
                     /locus_tag="ACHGNW_000037"
     CDS             31803..33023
                     /locus_tag="ACHGNW_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN6924396.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33179..34504
                     /gene="ftsA"
                     /locus_tag="ACHGNW_000038"
     CDS             33179..34504
                     /gene="ftsA"
                     /locus_tag="ACHGNW_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN6924397.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34527..35768
                     /gene="ftsZ"
                     /locus_tag="ACHGNW_000039"
     CDS             34527..35768
                     /gene="ftsZ"
                     /locus_tag="ACHGNW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN6924398.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35785..36462
                     /locus_tag="ACHGNW_000040"
     CDS             35785..36462
                     /locus_tag="ACHGNW_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN6924399.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36476..37075
                     /locus_tag="ACHGNW_000041"
     CDS             36476..37075
                     /locus_tag="ACHGNW_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN6924400.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37156..37377
                     /locus_tag="ACHGNW_000042"
     CDS             37156..37377
                     /locus_tag="ACHGNW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN6924401.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37426..38208
                     /locus_tag="ACHGNW_000043"
     CDS             37426..38208
                     /locus_tag="ACHGNW_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN6924402.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38229..38936
                     /locus_tag="ACHGNW_000044"
     CDS             38229..38936
                     /locus_tag="ACHGNW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN6924403.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39198..41996
                     /gene="ileS"
                     /locus_tag="ACHGNW_000045"
     CDS             39198..41996
                     /gene="ileS"
                     /locus_tag="ACHGNW_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN6924404.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42045..43571)
                     /gene="zwf"
                     /locus_tag="ACHGNW_000046"
     CDS             complement(42045..43571)
                     /gene="zwf"
                     /locus_tag="ACHGNW_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN6924405.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43586..44233)
                     /locus_tag="ACHGNW_000047"
     CDS             complement(43586..44233)
                     /locus_tag="ACHGNW_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6924406.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44417..44746)
                     /locus_tag="ACHGNW_000048"
     CDS             complement(44417..44746)
                     /locus_tag="ACHGNW_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN6924407.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44923..45651
                     /gene="sfsA"
                     /locus_tag="ACHGNW_000049"
     CDS             44923..45651
                     /gene="sfsA"
                     /locus_tag="ACHGNW_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN6924408.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45667..46680
                     /locus_tag="ACHGNW_000050"
     CDS             45667..46680
                     /locus_tag="ACHGNW_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6924409.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46680..47957
                     /locus_tag="ACHGNW_000051"
     CDS             46680..47957
                     /locus_tag="ACHGNW_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6924410.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48020..50722
                     /locus_tag="ACHGNW_000052"
     CDS             48020..50722
                     /locus_tag="ACHGNW_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6924411.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50874..51191
                     /locus_tag="ACHGNW_000053"
     CDS             50874..51191
                     /locus_tag="ACHGNW_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6924412.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51221..51541
                     /locus_tag="ACHGNW_000054"
     CDS             51221..51541
                     /locus_tag="ACHGNW_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6924413.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51649..53109
                     /locus_tag="ACHGNW_000055"
     CDS             51649..53109
                     /locus_tag="ACHGNW_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6924414.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53177..53398
                     /locus_tag="ACHGNW_000056"
     CDS             53177..53398
                     /locus_tag="ACHGNW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924415.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53429..53610
                     /locus_tag="ACHGNW_000057"
                     /pseudo
     CDS             53429..53610
                     /locus_tag="ACHGNW_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
     gene            53610..54023
                     /locus_tag="ACHGNW_000058"
     CDS             53610..54023
                     /locus_tag="ACHGNW_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN6924416.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54146..55327
                     /locus_tag="ACHGNW_000059"
     CDS             54146..55327
                     /locus_tag="ACHGNW_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924417.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55398..55562)
                     /locus_tag="ACHGNW_000060"
     CDS             complement(55398..55562)
                     /locus_tag="ACHGNW_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924418.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55716..55789
                     /locus_tag="ACHGNW_000061"
     tRNA            55716..55789
                     /locus_tag="ACHGNW_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55750..55752,aa:Arg,seq:cct)
     gene            complement(55835..55948)
                     /locus_tag="ACHGNW_000062"
                     /pseudo
     CDS             complement(55835..55948)
                     /locus_tag="ACHGNW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(56331..57563)
                     /locus_tag="ACHGNW_000063"
     CDS             complement(56331..57563)
                     /locus_tag="ACHGNW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN6924419.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57817..58386
                     /locus_tag="ACHGNW_000064"
     CDS             57817..58386
                     /locus_tag="ACHGNW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6924420.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58564..59004)
                     /locus_tag="ACHGNW_000065"
     CDS             complement(58564..59004)
                     /locus_tag="ACHGNW_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN6924421.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59162..59926
                     /gene="map"
                     /locus_tag="ACHGNW_000066"
     CDS             59162..59926
                     /gene="map"
                     /locus_tag="ACHGNW_000066"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN6924422.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59958..60881
                     /locus_tag="ACHGNW_000067"
     CDS             59958..60881
                     /locus_tag="ACHGNW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN6924423.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60957..62096
                     /locus_tag="ACHGNW_000068"
     CDS             60957..62096
                     /locus_tag="ACHGNW_000068"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN6924424.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62089..62889
                     /locus_tag="ACHGNW_000069"
     CDS             62089..62889
                     /locus_tag="ACHGNW_000069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6924425.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62889..63716
                     /locus_tag="ACHGNW_000070"
     CDS             62889..63716
                     /locus_tag="ACHGNW_000070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6924426.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63721..64428
                     /locus_tag="ACHGNW_000071"
     CDS             63721..64428
                     /locus_tag="ACHGNW_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6924427.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            64528..65394
                     /gene="rfbA"
                     /locus_tag="ACHGNW_000072"
     CDS             64528..65394
                     /gene="rfbA"
                     /locus_tag="ACHGNW_000072"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN6924428.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65408..65980
                     /gene="rfbC"
                     /locus_tag="ACHGNW_000073"
     CDS             65408..65980
                     /gene="rfbC"
                     /locus_tag="ACHGNW_000073"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN6924429.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66002..67030
                     /gene="rfbB"
                     /locus_tag="ACHGNW_000074"
     CDS             66002..67030
                     /gene="rfbB"
                     /locus_tag="ACHGNW_000074"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN6924430.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67128..67979
                     /gene="rfbD"
                     /locus_tag="ACHGNW_000075"
     CDS             67128..67979
                     /gene="rfbD"
                     /locus_tag="ACHGNW_000075"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN6924431.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68014..70047
                     /locus_tag="ACHGNW_000076"
     CDS             68014..70047
                     /locus_tag="ACHGNW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924432.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70091..71371
                     /locus_tag="ACHGNW_000077"
     CDS             70091..71371
                     /locus_tag="ACHGNW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN6924433.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71581..72387
                     /locus_tag="ACHGNW_000078"
     CDS             71581..72387
                     /locus_tag="ACHGNW_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924434.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72399..73619
                     /locus_tag="ACHGNW_000079"
     CDS             72399..73619
                     /locus_tag="ACHGNW_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924435.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73609..75195
                     /locus_tag="ACHGNW_000080"
     CDS             73609..75195
                     /locus_tag="ACHGNW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6924436.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75234..77372
                     /locus_tag="ACHGNW_000081"
     CDS             75234..77372
                     /locus_tag="ACHGNW_000081"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6924437.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77740..78723)
                     /locus_tag="ACHGNW_000082"
     CDS             complement(77740..78723)
                     /locus_tag="ACHGNW_000082"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6924438.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79101..80492
                     /locus_tag="ACHGNW_000083"
     CDS             79101..80492
                     /locus_tag="ACHGNW_000083"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6924439.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80508..81548
                     /locus_tag="ACHGNW_000084"
     CDS             80508..81548
                     /locus_tag="ACHGNW_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6924440.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81567..82652
                     /locus_tag="ACHGNW_000085"
     CDS             81567..82652
                     /locus_tag="ACHGNW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN6924441.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82685..>82976
                     /locus_tag="ACHGNW_000086"
     CDS             82685..>82976
                     /locus_tag="ACHGNW_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6924442.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
BASE COUNT        27137 a        13954 c        17560 g        24325 t
ORIGIN      
        1 gttctataac gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa
       61 tttttggttt gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag
      121 gaaatctgat atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta
      181 catattatga aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca
      241 accacgaaga tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa
      301 aaaagagaaa tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt
      361 caaaaacggc atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag
      421 gaatcacagc atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat
      481 ttttgtaaga atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat
      541 gaaacacgag aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg
      601 aagaggagga gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta
      661 ttggatttta tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa
      721 gaagctcatg tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt
      781 ttacttggac agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca
      841 tcaagtgtca tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc
      901 gatgtcatga tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg
      961 actttgcttg aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt
     1021 atttttatgg atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg
     1081 ttaagaaaag gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc
     1141 ccagatgaag agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt
     1201 cgtgttgtga tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg
     1261 atcttaatga caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt
     1321 agataatcga ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta
     1381 agtcaattaa aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct
     1441 cctgttttca attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg
     1501 atttagtagt tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga
     1561 cagaagcaat ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc
     1621 agctggatag agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac
     1681 gctaaaaaac cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata
     1741 ttgtttgttt tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg
     1801 agcaaaaaaa taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt
     1861 ttagtgactc tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact
     1921 gattctgaca cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct
     1981 gttcaaacat tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt
     2041 tatggagctt atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt
     2101 ttttttgttg cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt
     2161 gggctagctc tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt
     2221 atctaacagg aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg
     2281 aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt
     2341 cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg
     2401 ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg
     2461 gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt
     2521 tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt
     2581 ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat
     2641 ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg
     2701 taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt
     2761 tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga
     2821 taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt
     2881 ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg
     2941 caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa
     3001 cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag
     3061 tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg
     3121 cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag
     3181 gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc
     3241 gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag
     3301 ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag
     3361 cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg
     3421 agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta
     3481 cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt
     3541 ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg
     3601 ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt
     3661 tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg
     3721 tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg
     3781 aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag
     3841 aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag
     3901 ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg
     3961 aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata
     4021 ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg
     4081 attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca
     4141 ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg
     4201 atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt
     4261 cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa
     4321 tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt
     4381 attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc
     4441 ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct
     4501 aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt
     4561 aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa
     4621 agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat
     4681 tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa
     4741 agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg
     4801 gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca
     4861 gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga
     4921 aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg
     4981 aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt
     5041 ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag
     5101 gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca
     5161 aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag
     5221 tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa
     5281 aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc
     5341 tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag
     5401 cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga
     5461 agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat
     5521 tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc
     5581 agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc
     5641 tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga
     5701 ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc
     5761 aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg
     5821 agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa
     5881 caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     5941 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     6001 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     6061 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     6121 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     6181 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     6241 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     6301 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     6361 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     6421 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     6481 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     6541 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     6601 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     6661 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     6721 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     6781 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     6841 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     6901 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     6961 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     7021 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     7081 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     7141 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     7201 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     7261 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     7321 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     7381 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     7441 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     7501 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     7561 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     7621 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     7681 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     7741 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     7801 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     7861 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     7921 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     7981 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     8041 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     8101 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     8161 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     8221 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     8281 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     8341 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     8401 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     8461 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     8521 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     8581 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     8641 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     8701 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     8761 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     8821 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     8881 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     8941 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     9001 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     9061 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     9121 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     9181 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     9241 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     9301 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     9361 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     9421 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     9481 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     9541 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     9601 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     9661 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     9721 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     9781 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     9841 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     9901 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     9961 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
    10021 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
    10081 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
    10141 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
    10201 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
    10261 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
    10321 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
    10381 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
    10441 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
    10501 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
    10561 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
    10621 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
    10681 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
    10741 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
    10801 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
    10861 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
    10921 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
    10981 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
    11041 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
    11101 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
    11161 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
    11221 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
    11281 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
    11341 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
    11401 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
    11461 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
    11521 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
    11581 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
    11641 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
    11701 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
    11761 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
    11821 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
    11881 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
    11941 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
    12001 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
    12061 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
    12121 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
    12181 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
    12241 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
    12301 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
    12361 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
    12421 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
    12481 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
    12541 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
    12601 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
    12661 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
    12721 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
    12781 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
    12841 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
    12901 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
    12961 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
    13021 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
    13081 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
    13141 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
    13201 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
    13261 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
    13321 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
    13381 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
    13441 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
    13501 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
    13561 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
    13621 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
    13681 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
    13741 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
    13801 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
    13861 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
    13921 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
    13981 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
    14041 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
    14101 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
    14161 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
    14221 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
    14281 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
    14341 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
    14401 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
    14461 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
    14521 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
    14581 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
    14641 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
    14701 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    14761 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    14821 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    14881 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    14941 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    15001 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    15061 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    15121 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    15181 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    15241 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    15301 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    15361 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    15421 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    15481 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    15541 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    15601 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    15661 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    15721 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    15781 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    15841 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    15901 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    15961 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    16021 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    16081 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    16141 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    16201 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    16261 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    16321 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    16381 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    16441 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    16501 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    16561 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    16621 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    16681 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    16741 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    16801 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    16861 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    16921 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    16981 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    17041 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    17101 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    17161 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    17221 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    17281 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    17341 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    17401 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    17461 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    17521 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    17581 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    17641 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    17701 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    17761 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    17821 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    17881 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    17941 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    18001 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    18061 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    18121 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    18181 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    18241 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    18301 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    18361 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    18421 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    18481 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    18541 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    18601 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    18661 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    18721 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    18781 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    18841 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    18901 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    18961 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    19021 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    19081 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    19141 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    19201 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    19261 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    19321 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    19381 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    19441 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    19501 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    19561 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    19621 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    19681 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    19741 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    19801 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    19861 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    19921 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    19981 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    20041 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    20101 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    20161 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    20221 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    20281 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    20341 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    20401 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    20461 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    20521 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    20581 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    20641 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    20701 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    20761 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    20821 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    20881 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    20941 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    21001 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    21061 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    21121 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    21181 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    21241 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    21301 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    21361 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    21421 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    21481 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    21541 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    21601 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    21661 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    21721 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    21781 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    21841 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    21901 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    21961 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    22021 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    22081 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    22141 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    22201 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    22261 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    22321 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    22381 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    22441 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    22501 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    22561 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    22621 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    22681 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    22741 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    22801 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    22861 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    22921 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    22981 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    23041 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    23101 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    23161 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    23221 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    23281 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    23341 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    23401 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    23461 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    23521 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    23581 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    23641 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    23701 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    23761 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    23821 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    23881 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    23941 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    24001 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    24061 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    24121 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    24181 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    24241 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    24301 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    24361 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    24421 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    24481 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    24541 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    24601 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    24661 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    24721 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    24781 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    24841 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
    24901 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    24961 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    25021 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    25081 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    25141 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    25201 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    25261 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    25321 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    25381 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    25441 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    25501 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    25561 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    25621 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    25681 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    25741 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    25801 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    25861 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    25921 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    25981 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    26041 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    26101 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
    26161 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    26221 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    26281 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    26341 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    26401 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    26461 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    26521 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    26581 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    26641 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    26701 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    26761 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    26821 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    26881 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    26941 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    27001 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    27061 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    27121 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    27181 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    27241 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    27301 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    27361 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    27421 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    27481 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    27541 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    27601 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    27661 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    27721 gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    27781 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    27841 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    27901 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    27961 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    28021 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    28081 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    28141 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    28201 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    28261 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    28321 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    28381 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    28441 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    28501 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    28561 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    28621 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    28681 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    28741 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    28801 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    28861 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    28921 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    28981 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    29041 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    29101 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    29161 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    29221 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    29281 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    29341 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    29401 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    29461 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    29521 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    29581 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    29641 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    29701 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    29761 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    29821 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    29881 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    29941 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    30001 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    30061 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    30121 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    30181 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    30241 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    30301 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    30361 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    30421 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    30481 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    30541 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    30601 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    30661 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
    30721 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
    30781 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
    30841 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
    30901 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
    30961 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
    31021 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
    31081 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
    31141 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
    31201 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
    31261 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
    31321 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
    31381 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
    31441 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
    31501 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
    31561 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
    31621 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
    31681 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
    31741 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
    31801 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
    31861 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
    31921 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
    31981 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
    32041 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
    32101 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
    32161 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
    32221 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
    32281 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
    32341 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
    32401 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    32461 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    32521 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    32581 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    32641 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    32701 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    32761 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    32821 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    32881 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    32941 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    33001 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    33061 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    33121 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    33181 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    33241 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    33301 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    33361 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    33421 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    33481 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    33541 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    33601 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    33661 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    33721 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    33781 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    33841 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    33901 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    33961 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    34021 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    34081 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    34141 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    34201 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    34261 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    34321 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    34381 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    34441 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    34501 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    34561 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    34621 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    34681 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    34741 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    34801 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    34861 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    34921 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    34981 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    35041 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    35101 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    35161 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    35221 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    35281 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    35341 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    35401 atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca
    35461 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    35521 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    35581 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    35641 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    35701 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    35761 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    35821 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    35881 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    35941 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    36001 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    36061 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    36121 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    36181 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    36241 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    36301 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    36361 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    36421 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    36481 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    36541 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    36601 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    36661 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    36721 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    36781 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    36841 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    36901 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    36961 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    37021 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    37081 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    37141 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    37201 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    37261 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    37321 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    37381 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    37441 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    37501 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    37561 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    37621 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    37681 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    37741 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    37801 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    37861 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    37921 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    37981 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    38041 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    38101 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    38161 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    38221 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    38281 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    38341 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    38401 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    38461 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    38521 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    38581 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    38641 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    38701 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    38761 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    38821 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    38881 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    38941 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    39001 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    39061 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    39121 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    39181 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    39241 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    39301 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    39361 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    39421 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    39481 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    39541 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    39601 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    39661 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    39721 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    39781 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    39841 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    39901 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    39961 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    40021 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    40081 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    40141 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    40201 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    40261 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    40321 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    40381 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    40441 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    40501 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    40561 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    40621 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    40681 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    40741 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    40801 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    40861 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    40921 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    40981 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    41041 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    41101 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    41161 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    41221 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    41281 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    41341 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    41401 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    41461 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    41521 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    41581 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    41641 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    41701 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    41761 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    41821 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    41881 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    41941 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    42001 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    42061 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    42121 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    42181 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    42241 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    42301 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    42361 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    42421 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    42481 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    42541 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    42601 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    42661 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    42721 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    42781 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    42841 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    42901 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    42961 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    43021 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    43081 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    43141 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    43201 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    43261 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    43321 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    43381 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    43441 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    43501 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    43561 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    43621 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    43681 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    43741 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    43801 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    43861 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    43921 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    43981 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    44041 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    44101 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    44161 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    44221 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    44281 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    44341 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    44401 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    44461 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    44521 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    44581 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    44641 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    44701 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    44761 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    44821 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    44881 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    44941 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    45001 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    45061 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    45121 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    45181 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    45241 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    45301 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    45361 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    45421 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    45481 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    45541 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    45601 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    45661 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    45721 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    45781 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    45841 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    45901 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    45961 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    46021 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    46081 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    46141 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    46201 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    46261 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    46321 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    46381 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    46441 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    46501 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    46561 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    46621 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    46681 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    46741 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    46801 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    46861 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    46921 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    46981 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    47041 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    47101 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    47161 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    47221 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    47281 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    47341 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    47401 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    47461 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    47521 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    47581 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    47641 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    47701 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    47761 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    47821 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    47881 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    47941 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    48001 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    48061 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    48121 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    48181 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    48241 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    48301 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    48361 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    48421 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    48481 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    48541 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    48601 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    48661 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    48721 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    48781 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    48841 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    48901 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    48961 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    49021 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    49081 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    49141 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    49201 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    49261 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    49321 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    49381 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    49441 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    49501 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    49561 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    49621 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    49681 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    49741 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    49801 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    49861 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    49921 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    49981 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    50041 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    50101 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    50161 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    50221 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    50281 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    50341 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    50401 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    50461 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    50521 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    50581 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    50641 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    50701 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    50761 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    50821 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    50881 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    50941 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    51001 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    51061 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    51121 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    51181 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    51241 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    51301 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    51361 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    51421 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    51481 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    51541 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    51601 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    51661 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    51721 ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc
    51781 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    51841 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    51901 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    51961 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    52021 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    52081 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    52141 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    52201 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    52261 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    52321 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    52381 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga gccattccta
    52441 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    52501 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    52561 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    52621 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    52681 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    52741 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    52801 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    52861 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    52921 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    52981 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    53041 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    53101 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    53161 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    53221 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    53281 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    53341 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    53401 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    53461 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    53521 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    53581 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    53641 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    53701 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    53761 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    53821 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    53881 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    53941 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    54001 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    54061 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    54121 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    54181 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    54241 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    54301 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    54361 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    54421 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    54481 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    54541 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    54601 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    54661 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    54721 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    54781 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    54841 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    54901 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    54961 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    55021 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    55081 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    55141 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    55201 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    55261 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    55321 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    55381 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    55441 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    55501 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    55561 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    55621 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    55681 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    55741 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt acaaataacc
    55801 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
    55861 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
    55921 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
    55981 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
    56041 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
    56101 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
    56161 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
    56221 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
    56281 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
    56341 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
    56401 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
    56461 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
    56521 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
    56581 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
    56641 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
    56701 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
    56761 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
    56821 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
    56881 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
    56941 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
    57001 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
    57061 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
    57121 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
    57181 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
    57241 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
    57301 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
    57361 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
    57421 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
    57481 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
    57541 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
    57601 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
    57661 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
    57721 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
    57781 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
    57841 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
    57901 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
    57961 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
    58021 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
    58081 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
    58141 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
    58201 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
    58261 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
    58321 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
    58381 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
    58441 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
    58501 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
    58561 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
    58621 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
    58681 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
    58741 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
    58801 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
    58861 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
    58921 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
    58981 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
    59041 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
    59101 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
    59161 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
    59221 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
    59281 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
    59341 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
    59401 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
    59461 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
    59521 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
    59581 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
    59641 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
    59701 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
    59761 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
    59821 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
    59881 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
    59941 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
    60001 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
    60061 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
    60121 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
    60181 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
    60241 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
    60301 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
    60361 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
    60421 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
    60481 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
    60541 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
    60601 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
    60661 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
    60721 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
    60781 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
    60841 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
    60901 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
    60961 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
    61021 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
    61081 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
    61141 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
    61201 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
    61261 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
    61321 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
    61381 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
    61441 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
    61501 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
    61561 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
    61621 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
    61681 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
    61741 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
    61801 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
    61861 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
    61921 tcgtattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
    61981 tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc
    62041 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
    62101 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
    62161 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
    62221 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
    62281 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
    62341 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
    62401 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
    62461 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
    62521 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
    62581 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
    62641 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
    62701 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
    62761 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
    62821 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
    62881 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
    62941 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
    63001 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
    63061 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
    63121 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
    63181 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
    63241 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
    63301 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
    63361 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
    63421 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
    63481 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
    63541 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
    63601 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
    63661 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
    63721 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
    63781 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
    63841 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
    63901 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
    63961 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
    64021 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
    64081 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
    64141 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
    64201 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
    64261 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
    64321 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
    64381 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
    64441 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
    64501 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
    64561 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
    64621 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
    64681 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
    64741 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
    64801 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
    64861 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
    64921 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
    64981 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
    65041 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
    65101 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
    65161 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
    65221 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
    65281 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
    65341 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
    65401 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
    65461 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
    65521 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
    65581 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
    65641 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
    65701 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
    65761 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
    65821 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
    65881 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
    65941 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
    66001 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
    66061 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
    66121 aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt
    66181 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
    66241 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
    66301 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
    66361 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
    66421 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
    66481 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
    66541 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
    66601 tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
    66661 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
    66721 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
    66781 gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga
    66841 ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg
    66901 ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
    66961 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
    67021 tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg
    67081 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa
    67141 ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
    67201 catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt
    67261 atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata
    67321 aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg
    67381 tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt
    67441 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
    67501 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
    67561 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
    67621 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
    67681 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
    67741 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat
    67801 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    67861 cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac
    67921 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    67981 ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga
    68041 cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga cctgtttcta
    68101 aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac
    68161 ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact
    68221 gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct
    68281 ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac
    68341 ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa
    68401 ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg
    68461 atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg
    68521 aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg
    68581 tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt
    68641 tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg
    68701 gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg
    68761 tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg
    68821 gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt
    68881 catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga
    68941 aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat
    69001 tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat
    69061 cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc
    69121 gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt
    69181 accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta
    69241 agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat
    69301 atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca
    69361 tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa
    69421 accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga
    69481 cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca
    69541 gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc
    69601 gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca
    69661 acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag
    69721 aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct
    69781 tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg
    69841 ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta
    69901 actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac
    69961 ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact
    70021 caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt
    70081 agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt
    70141 cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt
    70201 tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat
    70261 tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc
    70321 gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg
    70381 agacttactg tttcctttgg ttattttatt ttgtttagta attaaatgga tcgagtggac
    70441 aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata
    70501 tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc
    70561 gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt
    70621 attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat
    70681 agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc
    70741 gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc
    70801 gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc
    70861 tgtctctgaa aaagtcaatt tgtttaccga tcgagaagat atttggcata aattctatca
    70921 gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg
    70981 aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata tctatggatt
    71041 gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa
    71101 agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca
    71161 gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt
    71221 ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct atgttatgga
    71281 agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc
    71341 tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac
    71401 aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac
    71461 gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc
    71521 aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa
    71581 atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa attattgcta
    71641 cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg
    71701 gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg
    71761 aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt
    71821 ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact
    71881 tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc
    71941 aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt
    72001 atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc
    72061 ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa
    72121 ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat
    72181 cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa
    72241 ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg
    72301 tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg
    72361 aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac
    72421 taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag
    72481 ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg
    72541 tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta
    72601 caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag
    72661 gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg
    72721 aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg
    72781 tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct gtaaaaactt
    72841 attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg aatcccgata
    72901 ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta aaatgtatgg
    72961 aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatca
    73021 gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa gtcgtcgaat
    73081 atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa aaatcagtta
    73141 aaacagtgga tcggaataaa aaccaagtag aacggttcgc cgctccagat atcgtcgacg
    73201 tgacgaaagc ggaattgttg gataaagaca aaaatccggt ggatatgctg gaacaaggag
    73261 aaaaactata cgttaaagta acttatgaag taaaagatga tacaatcaaa aatccggttt
    73321 taggaattgc gctacgaaca gtcgacaatt tttatgtatg cggacttaat actctactag
    73381 atggtatcaa gattccttgg aaaaaaggag agaatatctt ttatctggag tatgaaaagt
    73441 taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg
    73501 ttcctttggt atataagaca aagtacatga acttgtttgt cagcggttct tatatcggag
    73561 aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat
    73621 tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca
    73681 ggaagtaaag ttttagagtt tggtcccgga aacggccgat taacgaaaca tttaatcgga
    73741 gcaaaacaat gtgaagtgag cattgttgag ttagataagg aattgttcga ttttgtcagc
    73801 gaatttgctc aagatggatt ttatggtgat atcgaaagtt ttgagtgggc aaactattat
    73861 gctggacaga catttgatta cgtcttgttt gcggatgttt tagaacattt ggtagatcct
    73921 ggaaaaacat tgaagaaagt cagagaattt ttgaatgaag aaggagagat cctgattaca
    73981 ttccctaact tggcgcataa ttctgtcatg atcgatttgt tcaataacca attgccgtgg
    74041 gcttcttatg gtctcctaga tgaaacccat aattcttttt atactcatga tggcttccaa
    74101 aaagtttttg aaaaagcggg attattcatt aatattgaag attatttgta cttagctgta
    74161 ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg ctatgatttc
    74221 aaaatgcgac catttggtga agtctaccaa tactttttct cattgatgaa acatccagta
    74281 gcacaaagtt ccattgcaga gccacaaaat tctaattatg taaaagtgtt agaagtaacc
    74341 cagcaaacga aacaagatga aacaatccaa caaataccat tcaataattt cacaggggaa
    74401 aatgagacac taagtttccc cgtatcagaa acaacagaaa gaatggtgtt tcgcttcgcg
    74461 caacaaccta gctttatcga attcagcgca gaagctgccg gagaaaaact aacatttatc
    74521 gattcgaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg gaaagaatta
    74581 ccagagttcg ttctaacgga tatttctggg aaagaagtga ctatccattg tcactatcgc
    74641 tttatcgggg aattgacacc aacaatgaaa gaattgttgg aaacgattcg tcccatggct
    74701 gaagtcacaa agcagctttc tgagaaaaat gacgaattag aaagagaaaa tcatcatctt
    74761 cttgaggaaa atacaagact agaccatacg ttaaaaacaa ccacaaatcg ctattgcaca
    74821 ttgttggaaa gtgatgaatg gacaataaaa catagactag gacgacgtaa gaaagaaact
    74881 tcgaaaaaaa tccaagaaaa agaactttct atatgtattg acgagaaaat ctgggatgca
    74941 gaaacaaaaa tattaaaaat catcggatgg ggaatcgcta attcggatcg ccagcccttg
    75001 tcctataagt tatcagctga ccaatcgcca tttttcgaag caattcctgt ttcacgagaa
    75061 gaagtcaatc aagcagaaca gctcgcaaaa ggaacagaag cgggatttga gttacgaatc
    75121 ctttgcgagc aagaacgttc attcttagtg gaagcagtag ctcaaaatgg ccaatcatgg
    75181 atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa
    75241 aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc
    75301 ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa
    75361 aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt ggatatcaat catttatata
    75421 atcttgatgt taagacccaa agcggtttca aggtacgttt atcagggaaa atgagaggaa
    75481 aagccatctt agacttccaa acagcgagac atcaaaatgg catagctgtg aaattaaacg
    75541 gtaaataccc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga
    75601 aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcgatccgt cgggtcaaat
    75661 tggaattgaa gccaggatcg atcgactatg cagagtggat cagtcgtcat gagaaaccag
    75721 atttgaaaga gcaaaggaaa gaagcacaag gatttgcgta ccgtccattg atttccatac
    75781 ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtactgg
    75841 atcaaacgta tgatcattgg gaactatgta tcagtgatga tgcttctact gatccagcta
    75901 tccgaaagtg tctggaatcc tatcaagcaa aagatgatag aatcaaagtc gttttccgaa
    75961 aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatagca gaaggagaat
    76021 ttatagcact cttagataat gatgatgaat taccgccatt tgcattatat gaagtggcaa
    76081 aagtgctaaa cgtacatcca gaactggatg tgatctacag tgacgaagat aaaatcgatg
    76141 cagatggcaa tcgctttgat ccgcatttca aagcagactg gtcgcctgat acattgatgg
    76201 gaaataatta tatctctcac ttaggcgtat atcgaacaag cattgttaaa gaacttggcg
    76261 gtttccgtaa aggctatgaa ggttctcaag attatgattt ggtccttcgt gtgacagaac
    76321 aaatccctgc agatcatatt taccacattg accgagtctt gtatcattgg cggacgatcc
    76381 caggatcaac tgcaagtaat ggcgaagcca aaagttatat ctatgattcc ggagtgaaag
    76441 cattgacaga tgccctgtct agaagaaaca tcaaaggaag cgtacgtcct ggcagaattt
    76501 ctggatttta tgagattgct tatgacgtct tgcaagagga tttggtcagt gtgatcattc
    76561 caacgaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt
    76621 cttattcaaa ttatgaaatc attattgcgg ataacggaag tacagatcca aaaatgcaag
    76681 aattatttgc agaatacaag catcagctga aagaccgctt tatcgtagaa ttgattgaca
    76741 ttccattcaa ttattctcga attaacaatt tagcagcaga aaaagcaaac ggcaaatact
    76801 ttttattctt gaataacgat acagaagtga ttgagccaga ttggatgacg gtgatggttt
    76861 cttatgcaca gtttgatcga atcggctgtg taggagcaaa actgttttat ccagatgata
    76921 caacgcagca tgcaggagta ttattaggaa tcggtggtgt tgcagggcat gcattgaata
    76981 actatgatcg aacacattgt ggttatttcg gccgattggt cattgacgtc aattacttag
    77041 cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtt aacgggtttg
    77101 atgaaacatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg
    77161 gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag
    77221 gttatgaaga tactccagaa aaacaaagac gttttgctgg tgaaatcaaa aaaatgcaag
    77281 ataaatggcc agcatatatt gcgcatgatc cattttacaa tgataattta acaaaagacg
    77341 gaatcggcga cttttcgttg agaccagatt aaactataat aaaaagctgt tatgttgtga
    77401 ttcgctcgca cataacggct ttttatagtt ttttgtcaaa aaaaggcaga aggtgccgac
    77461 tcaaaaaaat aagtcgaggt tgggacagaa gtgtttaact ccgagaaata agaagaaatt
    77521 cacgaaaatt gcttttcaaa tttttgtgaa tttcggctta tttaagaaga agttgcttct
    77581 gctcccaccg tttattcgtt tttaaagaat gagacaaaag tgtttcttac ttttgtccca
    77641 cgctcttatt catgctcaga gcgccaatag atacaaaatc atagccaaag tttcacgaac
    77701 cctagctgtc aaagaatcga tattttattt taatataggc tattttaaaa taaatggttt
    77761 aaggtattcc tctacaaaag ccactctatc aattatcatg ggtttaaaat aagaagtgat
    77821 ggagataacc agattgttct tagcgtcaat ataaagaaca tttccactat taccaattgc
    77881 tgaatatatt tcgttctcag aatctaatac ccaccataaa tagccatatc tcatattttg
    77941 taagtcttct aaagcactgg ttctaggttt gaacatctcg tttatccaca tgggtggtac
    78001 gatttgtttt cctttataca tccctttatt taggcataat acaccaattt ttgccagatc
    78061 acttgccgat aaacacaaac catatcctgc cgtcccgata tttagtggat cacaaaacca
    78121 gacattggtt ttaggagact tatccattgt aaactgctta tgttcttcag ctgtttcagc
    78181 ataataattg acatggtttg gtatccctat aggatcaaat agatacttat tggcaaaatc
    78241 aacagttgtt agcttacttg tctgatacaa caagccagtt aatatgtgta aacagacagt
    78301 ttgatagttg aattcgccgg ttataccttt tcttcctccc aataaatcta gtgatgtttt
    78361 tgtccaatct tcacttgaac agacttttgt ccatggatcg cctttacatt tgtagggtgc
    78421 tttcattgtt agcaagtgct tgattgtaat agcgtaaata gttttctccc cgcgtttcac
    78481 tttatactct ggaaaataat ctaatacttt atcatcgata cttttgatta atccttgctc
    78541 taaagcaatg ccaatcagca aagatacaat acttttcgtt gctgacatga tatgacaagt
    78601 atcgtctttt ttataaccat tccattcatc tgtataaact tctaccccat ccttatatgc
    78661 gacaatttga catatatttg gttgtcgaaa accaataaaa tcatgcaact cttgtttatt
    78721 cataagatat tacctcactt tcttctataa gcataatgtc attaaaaatc gatttcaatt
    78781 ttttcgattc ttcacgtaat acaggtattc ttatggacta gacagcgctt tgggaagatg
    78841 gattctctga aaaatataaa ggttcacttt atgagtggat aaaaaaagca gcaaatactt
    78901 cgacttcttt aatttaccaa cattagtgaa aatcgattca tctggagatt tgtaatttag
    78961 agtccttgtc ttgattagag gtgtataaag ctttttatta gacattttta aattccatta
    79021 caatttgctt ataaactagg tttttaaagg tgactatggt aagataatag agatattatt
    79081 ttgtgattag gagtgtatat atgaataaaa atggagaatg gagtgctgca cgacggatat
    79141 tcattatatt aatggatgtt attgtataca atgcagcaat atatagtagt ttcttactga
    79201 agtttcgtgg agagatccca ttacgaaatt tagaaacttt ccaacactca gcaattatta
    79261 tttcagtgat ttttattgcg ttgaacattt tgttgggtgc atatgttttt tataatcgaa
    79321 tgatcagtga catcgtgttt gtcacagtca tcggtcaggt attgatgaca ctagggatca
    79381 tggtagtgac ttttgcgggc cgctggtttg cttttccacg tcttgttatc ttactttcat
    79441 ttgttctcgg tacgcttttc ttaagtatct atcgtattct cgtatataaa ctttatttac
    79501 gcgtaagtcg agataaaaag gtcgtcatta ttgggttaga aaaggatgtc gcaccagcaa
    79561 tccaaaattt tggttcaaag aaaaacaaca aacataaagt cgaagcagtc attcttgata
    79621 actattatgc aaacatcaaa aatatgatta aagacatcga tattgtctat cttgcttctc
    79681 atatcgaaga aactgaaaaa atcaaaatct atcaattagt tactaaaaat gaaaaaaaat
    79741 tgtttttgaa tacaacattt gaaaacttga cattgatcaa tccaaatatt atgaatttcg
    79801 aagatgaaag tattattgaa gcatctggat ttagaatcca acctgaatat gaactattca
    79861 aacgtttgtt tgatatcatc gtttcacttg ttttattagt tattgcttca ccatttatgt
    79921 tattcacagc aattttagtc aaagtgacat caccgggacc aatcatctat aaacaagtgc
    79981 ggatcacaaa aaatcaaaag gaattttcaa tctataaatt ccgctcgatg accgccacag
    80041 ccgaagcaaa atctggacca gttttagcta agagcaatga tgcacgagta acgcctgtag
    80101 gtaaatttat tcgagctgtc cgctttgatg aattaccaca aatcttcaat gtgctgaaag
    80161 gcgatatgtc gatcgtagga ccacgtcctg aacgttcttt cttcgtcgat cagttcaatg
    80221 cagaaaaccc ttattactac ttacggcata atgttcgtgc tggtatcact gggtatgcac
    80281 aagtctatgg aaaatatgtc tctgattaca acagtaaatt acgttttgat ttactttata
    80341 tcaaaaaata ttcattgatg cttgatttaa agatcttgct gcaaaccatc aagatcctat
    80401 ttgataaaat gtcctctcaa ggacttgaag aagacgaaaa tgtgatctca ttggcagaaa
    80461 ttgcttttcc gaatgaaaaa atctttcgct agttgggagg cgtaaaaatg aacaatccga
    80521 aattcagcat aatcgtacca gtatacaatg cagaagcaac tattggtcga acgattgata
    80581 ttctaaaaga attggattat gaagagtacg aagtcgtttt agtaaatgat ggatcaacag
    80641 atcaaacaca ggaaatcatt caagaactga ttgaaaatga tgaacgtttt gaattgatta
    80701 ctacgaccaa tcaagggccc ggattagcaa gaaacgaagg aataagacag tcaaaaggag
    80761 aatatctctt attcttcgat gcagatgaca cgccagtaaa aacgatcttg tcagattatg
    80821 atcatttgct agaaaagagt ccaaaagcgg atcttgttgt tagttcgttt gtttttcgta
    80881 caatggacga gggaagaatc gtttccgaaa aagaaaactt agttacagaa tgtcactatc
    80941 taactcacga agagtttata aaagacatgt atcagttaat gaatgaacag ttgatgtatg
    81001 ttgtatggaa caaatgctat cgtagcgata ttctgaaaaa taaccaaatt ctatttaaaa
    81061 cttataatag ttgtgaggat cgaatattta acttacatta ttatgaatac tgtcatcaag
    81121 tgatcatgaa cccaaaagta gaatatattt atgaatttga aggtgggaag gggatcacta
    81181 atcaataccg acctaacaag ttcgaaacat ttaaagaatt ctattcatta gcaaatactg
    81241 taacagacga tattaataaa gacggtatgg cggcgcttct tttaaaaggg acaacttcag
    81301 ttattttttc tatttatagt acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa
    81361 agcagatatt gagtgatcca gtgatccgag aagccaagca aattgctaag acggattctt
    81421 cagcaaaaaa aataacaaag ttgctttaca atatgccagc tccactattt ttatctgctg
    81481 taaaagttgg aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag
    81541 tatattagtt aatggaggaa aatataatga aaagaatttt gcattttcag ggacgaatgg
    81601 gtttaggtgg agcagagtct ttcatgatga atttatatcg taaaattgat cgtacaaact
    81661 atcaatttga ttttttgatt tatgaagatt atgcagatgt tcaagactat cattcagaga
    81721 ttgaaagatt aggaggccgt atttttgttg tacctaatcc taagaaaaat atttttaaat
    81781 atttaataga agtgaataag ttactaaaaa aagaatcttt ttctatagtt cacaatcagg
    81841 tttattttgg cgggggaatt aatttatggt tagccaaaaa gaatggaatc agacagagaa
    81901 ttgctcatag ccatgcaact gaagatggaa aatctcaaaa tattgtaatg aatgtattgc
    81961 gtaaattttt aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag
    82021 aagctggtga aagtctgttt caaaaccatc cctttgagat agttcataat ggtattgatt
    82081 tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa ttggatatct
    82141 caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa aatcaatctt
    82201 atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac ttgattttag
    82261 taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg gatatagaga
    82321 aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct gtgatggatg
    82381 tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa gcacaagcag
    82441 caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta acaaataatg
    82501 tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta ataaaagagc
    82561 cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgtac actactgaac
    82621 aaatggagag tatatatagt agagatcatt aaaaatgttc aagatatttt aggagtgatt
    82681 atttttgaaa atatgttatt tgatattggc atacaatgat attggcaact taaagagatt
    82741 ggtcaatcga ttgaacgaaa atgcagtttt tattattcat atagataaaa aaactgatat
    82801 agaaccattt attgaagcat ttaaatcata tgataatgtt gaatttttat gtaataaaaa
    82861 ccgtgttaaa atttattggg gtggtttttc cattgtacaa gcagaaatca atttagtaaa
    82921 gagagcatta caaaatgaga aatatttaaa atatgtatta ttatctggtg ctgatt
//
LOCUS       DAZBTH010000002        82156 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000002 DAZBTH010000000
VERSION     DAZBTH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.002"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1402
                     /locus_tag="ACHGNW_000087"
     CDS             32..1402
                     /locus_tag="ACHGNW_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN6924443.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(1642..1725)
                     /locus_tag="ACHGNW_000088"
     tRNA            complement(1642..1725)
                     /locus_tag="ACHGNW_000088"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Leu,seq:gag)
     gene            1798..1959
                     /locus_tag="ACHGNW_000089"
     CDS             1798..1959
                     /locus_tag="ACHGNW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924444.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            2108..3325
                     /locus_tag="ACHGNW_000090"
     CDS             2108..3325
                     /locus_tag="ACHGNW_000090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6924445.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3509..4345
                     /locus_tag="ACHGNW_000091"
     CDS             3509..4345
                     /locus_tag="ACHGNW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6924446.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4421..5569
                     /gene="nagA"
                     /locus_tag="ACHGNW_000092"
     CDS             4421..5569
                     /gene="nagA"
                     /locus_tag="ACHGNW_000092"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN6924447.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5845..6846
                     /locus_tag="ACHGNW_000093"
     CDS             5845..6846
                     /locus_tag="ACHGNW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN6924448.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            6865..7884
                     /locus_tag="ACHGNW_000094"
     CDS             6865..7884
                     /locus_tag="ACHGNW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN6924449.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7986..8702)
                     /locus_tag="ACHGNW_000095"
     CDS             complement(7986..8702)
                     /locus_tag="ACHGNW_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6924450.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8671..9456)
                     /locus_tag="ACHGNW_000096"
     CDS             complement(8671..9456)
                     /locus_tag="ACHGNW_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6924451.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9447..10196)
                     /locus_tag="ACHGNW_000097"
     CDS             complement(9447..10196)
                     /locus_tag="ACHGNW_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6924452.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10494..11018
                     /locus_tag="ACHGNW_000098"
     CDS             10494..11018
                     /locus_tag="ACHGNW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN6924453.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            11034..12533
                     /locus_tag="ACHGNW_000099"
     CDS             11034..12533
                     /locus_tag="ACHGNW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN6924454.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12621..13223)
                     /locus_tag="ACHGNW_000100"
     CDS             complement(12621..13223)
                     /locus_tag="ACHGNW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6924455.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13327..14283
                     /locus_tag="ACHGNW_000101"
     CDS             13327..14283
                     /locus_tag="ACHGNW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN6924456.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14588..15544)
                     /locus_tag="ACHGNW_000102"
     CDS             complement(14588..15544)
                     /locus_tag="ACHGNW_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6924457.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15718..16278
                     /locus_tag="ACHGNW_000103"
     CDS             15718..16278
                     /locus_tag="ACHGNW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924458.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16271..17095
                     /locus_tag="ACHGNW_000104"
     CDS             16271..17095
                     /locus_tag="ACHGNW_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN6924459.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            17105..17722
                     /locus_tag="ACHGNW_000105"
     CDS             17105..17722
                     /locus_tag="ACHGNW_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6924460.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17740..18423
                     /locus_tag="ACHGNW_000106"
     CDS             17740..18423
                     /locus_tag="ACHGNW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN6924461.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            19106..20341
                     /locus_tag="ACHGNW_000107"
                     /pseudo
     CDS             19106..20341
                     /locus_tag="ACHGNW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            20349..21236
                     /locus_tag="ACHGNW_000108"
     CDS             20349..21236
                     /locus_tag="ACHGNW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924462.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            21253..22089
                     /locus_tag="ACHGNW_000109"
     CDS             21253..22089
                     /locus_tag="ACHGNW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924463.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            22092..22715
                     /locus_tag="ACHGNW_000110"
     CDS             22092..22715
                     /locus_tag="ACHGNW_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924464.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22712..24004
                     /locus_tag="ACHGNW_000111"
     CDS             22712..24004
                     /locus_tag="ACHGNW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6924465.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24363..24460
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24576..25157
                     /locus_tag="ACHGNW_000112"
     CDS             24576..25157
                     /locus_tag="ACHGNW_000112"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN6924466.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            25160..26467
                     /locus_tag="ACHGNW_000113"
     CDS             25160..26467
                     /locus_tag="ACHGNW_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN6924467.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26760..27248
                     /gene="purE"
                     /locus_tag="ACHGNW_000114"
     CDS             26760..27248
                     /gene="purE"
                     /locus_tag="ACHGNW_000114"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN6924468.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27241..28365
                     /gene="purK"
                     /locus_tag="ACHGNW_000115"
     CDS             27241..28365
                     /gene="purK"
                     /locus_tag="ACHGNW_000115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN6924469.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28378..29673
                     /gene="purB"
                     /locus_tag="ACHGNW_000116"
     CDS             28378..29673
                     /gene="purB"
                     /locus_tag="ACHGNW_000116"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN6924470.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29853..30230)
                     /locus_tag="ACHGNW_000117"
     CDS             complement(29853..30230)
                     /locus_tag="ACHGNW_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924471.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30486..31214
                     /gene="purC"
                     /locus_tag="ACHGNW_000118"
     CDS             30486..31214
                     /gene="purC"
                     /locus_tag="ACHGNW_000118"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN6924472.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31214..31468
                     /gene="purS"
                     /locus_tag="ACHGNW_000119"
     CDS             31214..31468
                     /gene="purS"
                     /locus_tag="ACHGNW_000119"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN6924473.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31470..32141
                     /gene="purQ"
                     /locus_tag="ACHGNW_000120"
     CDS             31470..32141
                     /gene="purQ"
                     /locus_tag="ACHGNW_000120"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN6924474.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            32142..34364
                     /gene="purL"
                     /locus_tag="ACHGNW_000121"
     CDS             32142..34364
                     /gene="purL"
                     /locus_tag="ACHGNW_000121"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN6924475.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34349..35788
                     /gene="purF"
                     /locus_tag="ACHGNW_000122"
     CDS             34349..35788
                     /gene="purF"
                     /locus_tag="ACHGNW_000122"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN6924476.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35811..36863
                     /gene="purM"
                     /locus_tag="ACHGNW_000123"
     CDS             35811..36863
                     /gene="purM"
                     /locus_tag="ACHGNW_000123"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN6924477.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36860..37438
                     /gene="purN"
                     /locus_tag="ACHGNW_000124"
     CDS             36860..37438
                     /gene="purN"
                     /locus_tag="ACHGNW_000124"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN6924478.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37435..38970
                     /gene="purH"
                     /locus_tag="ACHGNW_000125"
     CDS             37435..38970
                     /gene="purH"
                     /locus_tag="ACHGNW_000125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN6924479.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38986..40260
                     /gene="purD"
                     /locus_tag="ACHGNW_000126"
     CDS             38986..40260
                     /gene="purD"
                     /locus_tag="ACHGNW_000126"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN6924480.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40382..41296
                     /locus_tag="ACHGNW_000127"
     CDS             40382..41296
                     /locus_tag="ACHGNW_000127"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN6924481.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41429..42994)
                     /locus_tag="ACHGNW_000128"
     CDS             complement(41429..42994)
                     /locus_tag="ACHGNW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6924482.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42987..44438)
                     /locus_tag="ACHGNW_000129"
     CDS             complement(42987..44438)
                     /locus_tag="ACHGNW_000129"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6924483.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44452..45468)
                     /locus_tag="ACHGNW_000130"
     CDS             complement(44452..45468)
                     /locus_tag="ACHGNW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6924484.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45688..46776
                     /gene="chvE"
                     /locus_tag="ACHGNW_000131"
     CDS             45688..46776
                     /gene="chvE"
                     /locus_tag="ACHGNW_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6924485.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46830..48356
                     /gene="mmsA"
                     /locus_tag="ACHGNW_000132"
     CDS             46830..48356
                     /gene="mmsA"
                     /locus_tag="ACHGNW_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6924486.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48358..49563
                     /gene="mmsB"
                     /locus_tag="ACHGNW_000133"
     CDS             48358..49563
                     /gene="mmsB"
                     /locus_tag="ACHGNW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN6924487.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49820..50797
                     /gene="guaC"
                     /locus_tag="ACHGNW_000134"
     CDS             49820..50797
                     /gene="guaC"
                     /locus_tag="ACHGNW_000134"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN6924488.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            51061..52485
                     /locus_tag="ACHGNW_000135"
     CDS             51061..52485
                     /locus_tag="ACHGNW_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6924489.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(52527..52784)
                     /locus_tag="ACHGNW_000136"
     CDS             complement(52527..52784)
                     /locus_tag="ACHGNW_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924490.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            52946..54055
                     /gene="pdhA"
                     /locus_tag="ACHGNW_000137"
     CDS             52946..54055
                     /gene="pdhA"
                     /locus_tag="ACHGNW_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN6924491.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            54058..55035
                     /locus_tag="ACHGNW_000138"
     CDS             54058..55035
                     /locus_tag="ACHGNW_000138"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN6924492.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            55060..56703
                     /locus_tag="ACHGNW_000139"
     CDS             55060..56703
                     /locus_tag="ACHGNW_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN6924493.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56711..58117
                     /gene="lpdA"
                     /locus_tag="ACHGNW_000140"
     CDS             56711..58117
                     /gene="lpdA"
                     /locus_tag="ACHGNW_000140"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN6924494.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            58279..58554
                     /locus_tag="ACHGNW_000141"
     CDS             58279..58554
                     /locus_tag="ACHGNW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN6924495.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            58560..59327
                     /locus_tag="ACHGNW_000142"
     CDS             58560..59327
                     /locus_tag="ACHGNW_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN6924496.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            59495..61324
                     /gene="typA"
                     /locus_tag="ACHGNW_000143"
     CDS             59495..61324
                     /gene="typA"
                     /locus_tag="ACHGNW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN6924497.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            61585..63357
                     /gene="recQ"
                     /locus_tag="ACHGNW_000144"
     CDS             61585..63357
                     /gene="recQ"
                     /locus_tag="ACHGNW_000144"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN6924498.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            63471..63773
                     /locus_tag="ACHGNW_000145"
     CDS             63471..63773
                     /locus_tag="ACHGNW_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN6924499.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63776..64939
                     /locus_tag="ACHGNW_000146"
     CDS             63776..64939
                     /locus_tag="ACHGNW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6924500.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64965..68393
                     /locus_tag="ACHGNW_000147"
     CDS             64965..68393
                     /locus_tag="ACHGNW_000147"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN6924501.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            68414..69538
                     /locus_tag="ACHGNW_000148"
     CDS             68414..69538
                     /locus_tag="ACHGNW_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN6924502.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            69519..69965
                     /locus_tag="ACHGNW_000149"
     CDS             69519..69965
                     /locus_tag="ACHGNW_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6924503.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69949..70287
                     /locus_tag="ACHGNW_000150"
     CDS             69949..70287
                     /locus_tag="ACHGNW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN6924504.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            70406..70966
                     /gene="rsmD"
                     /locus_tag="ACHGNW_000151"
     CDS             70406..70966
                     /gene="rsmD"
                     /locus_tag="ACHGNW_000151"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN6924505.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70963..71454
                     /gene="coaD"
                     /locus_tag="ACHGNW_000152"
     CDS             70963..71454
                     /gene="coaD"
                     /locus_tag="ACHGNW_000152"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN6924506.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            71444..72487
                     /locus_tag="ACHGNW_000153"
     CDS             71444..72487
                     /locus_tag="ACHGNW_000153"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN6924507.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72646..73374
                     /locus_tag="ACHGNW_000154"
     CDS             72646..73374
                     /locus_tag="ACHGNW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN6924508.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            73490..73735
                     /locus_tag="ACHGNW_000155"
     CDS             73490..73735
                     /locus_tag="ACHGNW_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6924509.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73732..73950
                     /locus_tag="ACHGNW_000156"
     CDS             73732..73950
                     /locus_tag="ACHGNW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN6924510.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            74086..74625
                     /locus_tag="ACHGNW_000157"
     CDS             74086..74625
                     /locus_tag="ACHGNW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN6924511.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            74618..76891
                     /locus_tag="ACHGNW_000158"
     CDS             74618..76891
                     /locus_tag="ACHGNW_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN6924512.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76929..77975
                     /gene="holA"
                     /locus_tag="ACHGNW_000159"
     CDS             76929..77975
                     /gene="holA"
                     /locus_tag="ACHGNW_000159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN6924513.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(78064..78318)
                     /gene="rpsT"
                     /locus_tag="ACHGNW_000160"
     CDS             complement(78064..78318)
                     /gene="rpsT"
                     /locus_tag="ACHGNW_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN6924514.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78718..78987)
                     /locus_tag="ACHGNW_000161"
     CDS             complement(78718..78987)
                     /locus_tag="ACHGNW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924515.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(79249..79377)
                     /locus_tag="ACHGNW_000162"
     CDS             complement(79249..79377)
                     /locus_tag="ACHGNW_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924516.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(79457..79912)
                     /locus_tag="ACHGNW_000163"
     CDS             complement(79457..79912)
                     /locus_tag="ACHGNW_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN6924517.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(80050..81978)
                     /locus_tag="ACHGNW_000164"
     CDS             complement(80050..81978)
                     /locus_tag="ACHGNW_000164"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6924518.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26661 a        14081 c        17744 g        23670 t
ORIGIN      
        1 tctattagga aatataagaa ggagtataaa aatgaacaaa cataatagaa aaataacgaa
       61 taacaaagaa ctgatcttgt tactaatagg gatttggggt atctttttac tctttaatat
      121 gtatatgccg actatgagag cggatgacgt agtttatgca agtagattag atgaacttgg
      181 ctatttgggt gcttcgatag aacattataa gacatggagt tcaagaatta ttatagaact
      241 attcttaatg ttcttttcta aacactttat gctatggaaa ttattaaatt ctacgattat
      301 gttaggaatt gtagtattac tttgcaagta tgtttttgag aaaataaatg ttaaaaattt
      361 attattggtt tgctctatat actgtttaat accattgaca gtaatggggg aaaccggttg
      421 gagagcaacc accttgaact atcagtggcc agtagcattt agtttactaa ctttttatcc
      481 gttttttcaa ctcttaagag gagaagaaat aaatagaaaa atatattggg taagtattcc
      541 tttattgata tttttaacta atcaagaaca agtcaacgcc tgtttttttg ttctaacaag
      601 tatagttagt ttatatttga ttgtcaatgg acgatataat tataagttat cagtattttc
      661 aataataagc ttagcagaat taattttctc tttgacaact ccagggaatg cattacgtgc
      721 tgcacatgaa attaataaat ggtttcctga atataaaaat ttcaactttc ttaataagtt
      781 agatttaggt atttcatcat ttggaaagcc gtttttttta gacatgaata tcttattttt
      841 attgctattt ttcttaattt tccttctaac atataggaaa tgtcaaaact attatgtgcg
      901 tatattgact gcattaccgt ttttcttaaa cctgattatt tattttggta atacaatggg
      961 tcaaagcttt acttacgtca atggaaataa aagggcaatg atttggagta gtagtaacct
     1021 gaataatctc tttacagagt taggaacgaa actgtcactt ttttatccag gtacttggat
     1081 agctacttta gtagtcttgg cattattact atgtttaatt gttggaattt atttaagttt
     1141 tgacaataag aaaacttcta tttttttagt tattttgatg ataatgggat tttgttcaag
     1201 gttaattatg ggattttctc ccacagtatg ggcttccgga atgcggacgt actatatact
     1261 ttatgttgtt atcgccatct tagtactaat ggcagttaaa gagctaatga aaagcatgag
     1321 tgtacaaaaa aatgaattta tgcaatttgg attaaccgtg cttggtattt gtacctttat
     1381 aataacggtg ataaacagat aatatacaaa aaatccctcg ctttagtatt aggtgaggga
     1441 ttttactcgt ttttcgatta attgttttaa cacttgcaag tttctacgag tagcttgttt
     1501 ccgaccttca tatactcacc tcaggctgtt tacatgattg tacgttcttt ttgtaaagga
     1561 tacagtgata tgtatataat ggcataaaga aacgttcaaa caaaaaaact cgtgaagcaa
     1621 aattaacttc acgagttgta gtgcagatga tgggactcga acccacacga gctaacgctc
     1681 acatgaccct caatcatgct tgtctgccaa ttccaacaca tctgcgtaat aataacacac
     1741 taatgatagc gaggttttac atagttgtca agctcaaaaa tgggaggact tattaagatg
     1801 gagaaatatg acggagaatt ttcaggactg ggaatgatct taggtgtatt gattggactt
     1861 gcattcggac gttttttgct tggttttatg ctaggaatca tttgtggaat tgcaatggat
     1921 tgggcagcga atctatggaa tgactatcat gacaattaag ttctcaacgc ttgtttgtgt
     1981 ttttttaata cttacgtttt tcctaaataa gtggcaaaat tttctaggat ggagtatgat
     2041 attgaaatag taaataacaa aaaatttgga aagttctaac tttttgaaat gagggattgg
     2101 ctaagtgatg agagaatttg aaaaatccaa caaattagaa ggtgtcagtt acgatgttcg
     2161 cggtcccgtt ctagaagaag cggagcggat gcaagaagag ggcattagta ttttaaaatt
     2221 gaacacggga aatcccgcac cttttggatt tgaagcacca aacgaagtca ttcgtgattt
     2281 gatcatgaat gcccgtgact cagaaggcta ctcagattcc aaggggatct tttctgctag
     2341 aaaagcaatt gagcaatatt gccaactgaa acattttccg aatgtgacga tcaatgatat
     2401 ctacaccggc aacggagtga gcgaattgat cacgatgtgt atgcaggggt tgctggacaa
     2461 tggagatgaa gtactggttc caatgccgga ctatccgttg tggacggctt ctattgcatt
     2521 agctggaggc acacctgttc attatatttg cgatgaagag gcagaatggt atcctgatat
     2581 tgatgatatc aaatcaaaaa taacgagtcg gacaaaagct attgttatca ttaatcctaa
     2641 taatcctact ggggcacttt atccaaaaga attattagaa gaaatcgttg aagtagctcg
     2701 acaaaataac ctgattattt attccgacga aatctatgat cgcttagtga tggatggtct
     2761 agaacatatt ccgattgcta cattagcgcc tgatttgttc gttgttacgc tgaacggtct
     2821 atccaaatct catcgtgtag caggatttcg agtaggctgg atggtcctaa gtggagataa
     2881 gtcacacgtc aaaggctata tagaagggct gaatatgttg tcctccatgc gtttgtgttc
     2941 caatgtattg tctcagcaaa ttatccaaac cgcgctaggc ggttatcaaa gtgtcgatga
     3001 gctgctcctg ccaggcggaa ggatctatga acaaagagaa tatatctata atgcaatcaa
     3061 tgatattcct ggtctttcag ccgtaaaacc aaaagctgct ttttatatat tcccaaaaat
     3121 tgatacaaag cgattcaata ttttggatga tgaaaaattc gttttggact ttttacatga
     3181 acatcatatt ctacttgtac atggtggtgg atttaattgg aaccagccag atcattttcg
     3241 aatcgtttat cttcctaaaa tggatgattt gaaacaaaca gctaagaaaa tgagagaatt
     3301 cctagcaacc tatcgccaaa aatgatttta aaaaccaaat ttttcagtag gagggccaca
     3361 gacaaacaca ctgaggctct ctttctagtg gagttttctt aaataactaa agtatattat
     3421 ttagttgcaa gtcttttctt gaagtttgcg acttcctaaa gtaaaatacg aatagaatgc
     3481 gaatacgcat attttgggga ggatacttat ggaaacgtta atcgagtcct ttggaacgat
     3541 cattaaagag attcgaaaag aacaaaaaat gacgcaaaaa atgctttcgc atgatatttg
     3601 ttcacaaagt gtattaagtc ggatcgaaaa taatgaggaa ctgccgaatg tattggtgat
     3661 gatgcagatt tgtcaacggc taggggtaac gattgatcat gtgatgaatc tctctcgaaa
     3721 aactactcgg acaaacgata aattatttga actgttggat agttgtttcc aaaaacgtga
     3781 ttatcggaag ttagaacaag tactaaaatc ttctgatatt tctgaaaatc tgtatcaagc
     3841 aactgacttt caacgctatt attattatct cggtgtttgc gaattcacgt tgcgtcaaaa
     3901 tatctctcag tccctttatt atttgaaaga agcattaagc tactctagcc aaaaagaaag
     3961 aatccatgtt tctgacacag aaatacaact gattagttgt atcggaaaaa tatatggtgt
     4021 ggcaggaaaa acggctgaag cacgctacta tttgagtcga agcatccaac tgctgcatca
     4081 atcagctgat gaacgatcaa aaagtgaatt ttcgcaaatt ttttataatt atggaaattt
     4141 cttattccat caaaatgaaa tcgacgaagc gttagaacag gtgaatcaag gaatctattg
     4201 ggctcaagag aagaatagct actattacct caatgattta tttgtgctta aaagcttaat
     4261 atacaagcgt aaagaagaac cagaaagagc agcattctat gaaggcttag cacaagctgt
     4321 gaaacaaatt tcaaaaaatt tgtaaatggt gtagaccatt cttgacttct tctctaaata
     4381 agagtacttt agaggtagaa aaacaaaggg agagaataga atgagaactt ttatctatgc
     4441 tgacaaattc tttttagcgt caggtgtcaa acatgcaggc tatcttgaaa ttaccgatgg
     4501 actttttggt gaatacacca aagaactacc tgagggagaa gtcaacatca ttgaccaaag
     4561 cggcaaatgg attgctcctg gtttggtaga tacacatatt cacggctata tgaatcatga
     4621 tgtaatggat aatgatgcag aaggcatcaa agtgatgtct gaaggtctgt tatcttgtgg
     4681 agtgacttca tttttaccaa caacattgac gtcaagcaaa gaacgtttaa aagatgtagc
     4741 tgagacaatc ggcaaaatgt atcaggaagc accaggagct aaaatcaaag gaatctattt
     4801 tgaaggtcct ttcttcacag aagaacacaa aggggcacaa aacccaagtt atttcgggga
     4861 tcctgatttg gatactttcc atgaatggca agaagcttca ggtggtttaa tcaaaaaaat
     4921 tgctttagca ccggaacgtc aaggtgtaga agagtttgtc aaacaagtga ctgaagaagg
     4981 agtagttgtt tctctaggac acagtaatgc tacactagaa gaagcgcaaa aagctgttga
     5041 agcaggcgca agtgtatttg tccatgcttt caatggtatg cgtggattga atcatcgcga
     5101 accaggtatg gtgggtgcgt tattatcttt agaacatgtc ttttctgaat tgatttgtga
     5161 tggccatcat gtacatccaa atgctgcggg aatcctaatg gaaaaagcag gacatgatca
     5221 tgtcgcatta attacagact gtatgatggc cggtgggatg cctgatggaa attacaattt
     5281 aggcgaattt ccagtagtag taaaagaagg aacagcacgt ctagaatctg gcaacttagc
     5341 tggaagtata ttgaaattga aagaagcaat taaaaatgtt gtagactggg gattagcaac
     5401 accagaacaa gcagtcatga tggctagtct gattccagca atcagctgta aaatcgatga
     5461 tacatgtgga aaaatcgctc aaggacgcga tgcagacttt atcgtattga accctgatat
     5521 gacattggaa gcaacttatc tagatggcgt ggaacgtttc cacgcataat ataatttaag
     5581 ttaaccttaa aactaaactt cttaagtttg gttttaaggt ttttttgtgt tctagtaaaa
     5641 atcactttac ataagtttca aaagtttctt taatttccta ttgttcaata agtaacaatg
     5701 taaagataaa attaaaaatg ttaatacgta ttagcaaaat aaaagctttt ttattgtgaa
     5761 taaatgaaca taattaggag gagaaagatg aacttatttg acatttttcg atcgactttg
     5821 acagatatgt cgattgtcag tgcaatcact tcaacagttt ttattatatt attagggttt
     5881 gtttgtagaa aaaaggggat tttttctgaa tctgttggaa aaattctatc gaaagtcgtc
     5941 ttaacagtgg ctttgccagc actggcattt cgagctttca tgcaagatat caatacagaa
     6001 ttactgatgc aaggaatcaa tgtcttgatt tggggattag ttatttatat tattcttatt
     6061 ctagtttcaa aacctttttt cttaagctat aaaggggata agcaaacaac gttgcgtgtc
     6121 ctgacgatct ttggttctac gacctttttc ggaacgccta tcgtcagtgc gatttatggt
     6181 cctgtcggcg tgatgtattc ctcaattttc aatattggct accgaatctt tttgtattct
     6241 tatggataca taaaaatgag tggcttgaag atggaagcta aaaatatcaa agcgatgttc
     6301 gtcaatccaa tcattatcgc tacttttgcc ggccttttca tttggctgtt ccaagggtat
     6361 ctgccacaag tgtcagttac aggtgcagaa ggagagataa gccaagtagc gtttttgcga
     6421 attgaccaga ctgcaccgtg gctatttcag ccgatggatt atttagctgg tttaagttca
     6481 ccattggcgt ggctagcaat cggcgcaaca ttaggtgaag taagcttcaa aaaagctgca
     6541 tctgaaaaaa cttcatggta ttacagtcta gtcaaagtgg tatttgtacc tgcattgaat
     6601 atcgtgattc tagcagtttt gacggcaacg aatattctac cagtcaattt tgaagcactg
     6661 gcaacaatcg ttatcatgat ggctacacca gctgcaacag tggcagcagc ctatgctatc
     6721 agttttgata gggaggcact tttagcctcc aatgcatcct tactgtcaac tatttttgct
     6781 gtggtactga cacctgtatg gatcgttgta ttacaaatga ttcatggcat aggtttattt
     6841 tcataagaaa tggaggaaga aagtatgaga atcgcatgtt acggtgttcg tccgaacgag
     6901 atagattttt ttgaaaaatt gaatatttac cattatgatc tcagtcttta tgaagaactg
     6961 ttgacacatg acaatatcgt gtcagcaaag gaccatgatg ctgtattgtt gaggggaaat
     7021 tgtatagcag atgagaccaa tcttgcaaaa atgcaggaat atgggattcg ttatgttttt
     7081 acacggactg tgggagtcaa tcatatcgat ctacaagcgg cagctgattt tggtatgaca
     7141 gtagcccgtg taccatcgta ttcgccaaat gcgatagcag agttatcttt gacttttgcc
     7201 atgatgcttt tgagaaatac tgcgtatacg acgatccgaa catctttcaa agattttcga
     7261 gtggatgagc aaatgtttag tcgtgaaatc agaaattgca cagtagggat cataggaaca
     7321 ggcaggatag ggttgacgga agctgttttg tttaagggac taggagccaa agtgatcggc
     7381 tacgatattc atcagacaga agctgctaaa aaagtattga cgttccactc gcttgaagag
     7441 ttattagcca aaagcgatat tgtcagtatt catgtacctc atatcccagg tgaaaacgat
     7501 caaatgatca atgaaggatt tttagctcaa atgaaaaaag gtagcatctt gattaataca
     7561 gcccgtggag agcttcaaga taatgaagct attttaagag ctttgaaaag caatcattta
     7621 gctggttttg caaccgatgt ttttgcaaat gaaacagagg tttttttccg atccttcaag
     7681 ccatgggaga cgattccaga ccctgctatc cagcaattag tagaaatgta ccctagagtt
     7741 cttgtaaccc cgcatgtagg ttcaaataca gatgaggcat tatcaaatat gatttcgaca
     7801 agtttcgaga actttcgtga aataatagaa actggaaaaa cgaaaaatga ggtatctctt
     7861 cctaaagccc gtcaattaag ataagaaaac atggatattc gtcaataaaa tgtttaaaaa
     7921 tagtccaagt aaacagttgt tacttctgta tacttggact attttgtatg ggataattcg
     7981 atgactcaac agtaatctgt gagctttttc cgcccattct ttgtcaattc ataacaggtt
     8041 agcggctggc cgatttccaa atatttgatt ctttcgttca atatatcttg ttctactaaa
     8101 aaattcaaat atttcttgac ggatatacgt gacagtgagc ttttttgagc cagttctttt
     8161 gttgaaaaaa tagtatcttc ttttagaata aatgcactta ttttttttaa agtcagtttt
     8221 gacagacctt taggcaaatc gatgaatgat ggtatttcgt gaatgggagt ggtgtggttg
     8281 aatacttgat cgatcaattt ctgatcggct ttttcagtcg tttggattgt ctgagagaaa
     8341 gcaagatact tttcgatcgc cagctgaaaa cgttgcaaag tgaacggttt gatcaaataa
     8401 tctaaggcac cgtagcggat cacattcttt atggtttcga tatcatctgc agcagtgatc
     8461 aaaatcactg ggatatcatt tccttgctgt ctaagccaag ataaaaaatg gataccactt
     8521 tcgccaggca tgtatacatc aagcagcagc agatctgcgt ttttttgtct gaaatatttt
     8581 tttccttctt ctacatttct gaaactttcg attacgttca tattagggat gctttccaca
     8641 aaagtacgat tcaattctgc aaccattgga tcatcttcga ttatcaggac atttgtcatc
     8701 attttgatct tccttttttg gtaattctag atagacgatt gtaccgccat ttgtttgttt
     8761 ggaaaattct aaaaatccac cgtggcgttt gacgattgaa tggatgagat tcaatccaaa
     8821 cccacgattg attcccttcg tcgaaaaacc tagcgtaaat attttttctg agatttcttc
     8881 tgatatcccg acaccgccat cgcagacggt cacgatgaat acttcagctt ctgtgtcata
     8941 gtttaaatga atccggacct gtccgtttct ttcatgtttt tgtagcacgg cttcttttgc
     9001 attatcaagt aagttcccta acacttgcaa aagtgaatgg atggagtcat ccatctccat
     9061 tttagggata aaagaatcgc tttctaacag catttcaaca gattgttctt tggcttcgtt
     9121 tattttgcct aataaaaaac cggcaattgc aggtactttg atcagacttg agacttggcc
     9181 aatctctttt ttataatttt gatttagctg ctggataaaa tgggtgactt cttcatactt
     9241 cttcaaatca atcaaaccag aaatgacatg aagtttattc atgaattcat gggtttgagc
     9301 tctcatggag tttatatatt gtttcgtccc cccaagctcc tccattaatt tcattacttc
     9361 tgatcgattt cgtaaagttg cgactgctcc taaaaaacgg tcattgctaa aaagaggtgt
     9421 aacgctagaa atagtttcta tcccatttaa atacatggat tgagtccgaa cggtcgtttg
     9481 atgctcaatc gtctctttaa acaaaacgtc atatagatta tcactgattt ttttcctact
     9541 tcgaaatcca atagctgctc tgtttggtag aaaatgtttt ttgcaggtgt attgatcaag
     9601 aggatagttt tatcagaaga gatggctaag attccttcat ctacttcatt ttcgataatc
     9661 gttttttcac ttaactgttc tgctatttct tgtggttcaa gtccaagcag tacttttttc
     9721 attttttggg caagaaaaac ggcgcctatg atgcctacgc taagtccaat aatcaatgaa
     9781 aaaacaatca ttttttgtcc atctttgact tgattgtcaa tcgtgttgtt tgttaagcca
     9841 acacaaactg caccaataat ctcttgatca taataaatag ggacgaaata gcggaagccg
     9901 aaccctaaca ttccttcttt ttcagagaaa aattcttgtc ctccaatcac ttttttcaca
     9961 tcttcatgac ttgagaaagg aagaccgatg taacttgggt ctgcgtgaga atatcgaatg
    10021 ccttcagggt ccatgacaac gataaaatct aaattcgatg caatcatgat ttcatttgtt
    10081 attccttgaa tcgcatgtgt cgggtcacca gtctgcagct ttttttggat gagtttatca
    10141 ttggcaacga ttttagcaat gctagagatt ttatctgaca cactagatcg gttcatcgaa
    10201 agaataaaat tcctgatcaa tatggcagaa acaatcaaag aaacgaatgt ggagaaacaa
    10261 acgacaagga tgatcgtgtt ctggagcttt aatggttttt tcttaaaaaa aggaaacata
    10321 ggctacctcg aaagaattta ttgtgaaaat tttaacatga aaacaaaaat ttaacaaatt
    10381 gtcaacgata aacacaacct tcctataaaa aacaccactg agtcggtaaa agaagctata
    10441 cagaattttc aaacattggt atgatagacg tagaaatgga aggaggcgtg tatatgatag
    10501 ggggaatcgc acttgagacg atttattggt atacattgct gatttgtgca ggattggcag
    10561 tcctattgat tatttttgga gatgtattcg attttgatgg cccgcttgat ccgatgctta
    10621 ttatcccttg gttaactttc acaagcttat ttggttttct aggtgagcgt ttaacagatc
    10681 aatcatcttt atttttattt attgctagtg gaggcatatc gatagtgttg gtatttttgc
    10741 ttaatttcta tatattgatg ccgatgaaac atgccgaatc aacgatgtcg tcttctgaaa
    10801 aaactctgga aggacaagta gctgttgtga tcacaccgat ccctgtacga ggtatgggag
    10861 aaattcagtt gaaaagtgtt accggttcca ttagtcgtcc agcttgtttt tacagccccc
    10921 aagaacagac agctgagcgg ggaacgaaag tattgatcat tgagatcaag gaaagaatct
    10981 gttatgtcat gccttatgaa agcatgataa aagcttaaag gagcgtgtgt taaatggaaa
    11041 taccagttgt ttttatcgcg atcgcaatcg cagctttgat tttactgatg ttgctgatcg
    11101 tctttgtagc gaagtaccag acagcaaaac cggatgaagc gttgatcatc agtggaagtt
    11161 atttaggaag caaaaatgtg cacattgatg aaagcaataa caaaatcaaa attgtgcgtg
    11221 gaggtggcgc attcgtcttg ccagtttttc aacgttcgaa tcggatcagt ctattatcga
    11281 gtaaattgga cgtctccacc cctgaagtgt ataccgaaca aggcgtaccg gtgatgtgtg
    11341 atggtacttc gatcatcaaa atcggttcat ctgtagaaga gattgctaca gcagcagaac
    11401 agtttttagg caagacaaga gaagaactgg aaaatgaagc aagagaagta ttagaaggac
    11461 acttgcgctc gattcttggt tctatgactg tagaagaaat ctatcaaaat cgcgataagt
    11521 tcagtcaaag cgtacaagaa gtcgcttctg tagaccttgc aaaaatgggg ttgatcatcg
    11581 tttctttcac tatcaaagag gtcagagaca aaaacggtta tcttgactct ttagggaaac
    11641 caagaatcgc ccaagtcaaa agagatgcag atatagcaga agcggaagct ctaaaagaaa
    11701 cgcgtatcaa aaaagctcaa gcagaaaaag aatcacaagc agctgaatta cagcgacaaa
    11761 cagagatcgc agaatcatta aaagaaaaag aactgaaatt agcaacctac aaacaagaac
    11821 aagatgttgc taaagcaaaa gcagaccaag cctacaattt agaaagtgcc cgagcacaac
    11881 aacaagtaat cgaacaggaa atgcagatta aaatcgtaga acgccaaaaa caaatcgaac
    11941 tggaagaaaa agaaatcaca cgtcgtgaaa agcaatatga ttcggaagtg aagaaaaaag
    12001 cagatgccga tcggtacgct aaagaacaag aagcacaagc tcaaaaagtc aaagaagtga
    12061 ctgaagccga agcagaacga ttccgtgtgg aagcactggc agaagcggaa gccaataaga
    12121 cacgtttagc tggacaagca gaagccgaag cagcactagc aaaaggaaaa gcagaagccg
    12181 aagcaaaaca aaagatcgcc aatgccttca aggaatatgg cgaagcagct gtcttgagta
    12241 tggttatcga tatgctaccg caattgatgc gcgaagcagc acagccgcta ggaaatatcg
    12301 aaaaaatctc tgttgtcgat acaggaagca gttcaggaga aaccagtgga gcgaaccgag
    12361 tgacaaatta tgccacaaat ttgctttctt ctactcaaga aacactaaaa gcgacaacag
    12421 gacttgactt gaaggaaatc gtagagaatt tgtcgacacg tgggactact agccaagtaa
    12481 atatgtatga agcgccaaaa gaggcagaac aaaactcgat gaaggaagaa taaagtagta
    12541 cacttcaccg ttcaatcaaa aatggaggaa gactttttaa agttcttctc tccatttttt
    12601 tattttattg caaaagaaaa ttagtcctcg ctatttttct tttgtttttg gatcttagct
    12661 tctatctctt gtaaatttgt tcttagcaat cgttctttct tttggagttc gaccaaatct
    12721 tctgatagtt cttccttctt ttctgtttca ggaggacgaa gaaagaaatt gataccgatt
    12781 gcaatgaatg tccctataaa agtatctagt acacgttgga tagcatacaa agcggattcg
    12841 ccttgaggga tgctcaacgc aatcagcaac aacgtagcta ttgcggaaat gataccggaa
    12901 ttattgttaa taccatctga cacgacgatc acaataataa ctagaatagg aagcaagatc
    12961 aattctacca aaaaatcatt gtgaaaatag cttttgacaa aaaaataaat gatagctaat
    13021 gtaccaccta atgtattgcc gatgatccta gactttccaa aagaaacggt tgtagtcaag
    13081 tcctgacgca aagaaaaaac agcagataat gcagcaatca tcgggattcc tcgttgagtc
    13141 aaatgaaaaa acaagataca taaaaaaaca gctaaggcac ttttgatcgt acgcatacct
    13201 agtcgaaaac ggccaaattg catagagatt ctcctttttg gtctttttac tatcatacct
    13261 aaacttttgt ctgatagcta gtttttcatt attcaagtat aattaaaata aaaaaggggg
    13321 aagtaaatgc gtataggaat gcgaacagtc aaaacagtga tcagtgtctt cttttcgatg
    13381 gtcacagcgg gtagcttatc gctgttgtat tggccagctg cagggatcat tgcattatta
    13441 agtgtaggaa ataccaagcg ctctacattg cgtacgggca tgaatcgaat cgcagccttt
    13501 atattagcta cggcaacagc actgttcagt ttttttgctg ttgggtatac tatttttggc
    13561 ttcagtcttt ttttattcct gttcatcccg cttgcctctc gcttcaaatt aaccgaaggg
    13621 atagtagtaa attcggtttt ggtcactcat tatttagtgg aggaaaacat gtcttggcag
    13681 ctgatcggca atgaagcggg attaatggcg attggattag tatttgcttt gcttgcaaat
    13741 gtttacatgc cagatcgtac gaagcagctg aaggaaaacc aaatacagat cgaaaaagaa
    13801 tttcgaaata ttttgagaca aatggcagaa tttcttttat cagaaaataa agaagatgtg
    13861 cagatcaaat gcgaacattt actgacattc attcgcgaaa gccaagaaga tgccagagaa
    13921 catcaagaaa actactggct cagacagccg ctatactacg aaacttactt ctctatgaga
    13981 agagcacagg ccaatgtgat taaagacatg ctggaaaatc tagagagaat ccagcaacca
    14041 gcttattatg gaaaacatat ctatggttta ttgatttata ctgcagaaac attttcagag
    14101 tcaaatgatg gcagacaaat tttaacacgt attgaagaag tttatgtatt gtatcggcaa
    14161 atgcctctgc cgactagtcg ccctgaattc gaggatcgag cagaattgtt ccagtttttg
    14221 cagtctttta aaagttttat tgaaatcaag gcagaattct cccaacaaaa aatccaaaaa
    14281 tagaggttgt gaattcagct aatattcagg tcaaaaagct gatttcacaa cctcttcttt
    14341 aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa
    14401 gctgtttttt gaaattgctc cttgtgactc ggagctgaac ggtaggctca caacctcttt
    14461 cttaaacggt gttcaaaagc tttcctctgc ggtaataagt ccagccaaac gaaaaatagg
    14521 aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
    14581 tttaaaatta catatttttc catagaactt gcatttgatc caagatttcg atgacatctt
    14641 ttgtcaagaa gagagagcga ttgggcaagt caccttcaat catgtgtacc atattcttta
    14701 tttcgtaatt cattgcttca gatgtattcc cgctttcgat gaattccttt gtaccatctg
    14761 taaataagat ctccgcacga tcagcacgcg ggtaatcagc aatcgtcaca taaccgttct
    14821 caaaggcaac gataccgact tttggcattt ttgcctgaaa agtcaagctg acagcagcaa
    14881 gttcattttc tttgttacgc aagatagtga cggattgttc gtccacgcct gtttcaaaag
    14941 gaaccattgt agaggcaact acttcaggtt gagagctcaa aaagaaacga gcgaaagata
    15001 cagcatacgt accgatatcc aacagagctc ctccagctaa ttctggattg aagaaccggt
    15061 ttttagggtc tggttctttg taactgccga aaggtgcttg gatcattttt aaagccccca
    15121 gttttcctgt atccattatt gagcgtaact gctgataaag tggcatgttg aagattgtca
    15181 tcgcttctgc taagatcaaa ttcttttctt ctgctaagcg catagcgtca gccagttctt
    15241 ttttattcat cgtgatggct ttttcacata gaacgtgttt gcctgcttcc aatgctgcca
    15301 agatatggtc gatatgctgt ctatttggta cagcaatgta cacgatgtcg atttcagcat
    15361 cgtctaacat ttcttgatag cttccgtaag cttttggaat attgtaacgg tgagcgaagt
    15421 tttccgcttt ttctgaagtt ctggaagcaa ctgctgttag ttcgctcgtc tcttgattga
    15481 agctttcggc aaactcatgt gcgatggtac caagaccaac gattccccat tgatattttt
    15541 tcatctgaaa acgctccttc atagttggat tgagtttctt gtctgtctat tattataaca
    15601 aaaatcacga agaataaaga ggagaggact tgaaacatta aaaaaaatga aagcagcgat
    15661 ttaagaggtg ctttttgatc aaaacttaag tagaatagtt aaaaggaggg gataaacgtg
    15721 catcaagtat atattgatct tattatgagt gtattattag aacagtttga aactgaaaaa
    15781 aattttttag atgaatactt gctgatcagt ccagaacaat ggcaaaaatg gaaagatggc
    15841 gagaaaaatc tgccatctga agcgatgcag aaagtaaaaa atcttttcac tgattatgaa
    15901 tggatgctaa cgcaaaagat tttgcgtcaa acgattattt ttcccgaaaa acgaaacgtt
    15961 gccgttgcag agtacaagca aatcaaaaca cgcatcgcgc aaaaatggct tagcagcaaa
    16021 gcagcaaaag tcgagctgat ccctttgaaa aataatcagc aggctggtag ataccttgac
    16081 ttgaaagtaa gtattcaata tgatgaatgg ggctatgatg atattttgaa ttttcgtctg
    16141 ccagcagatg ttcaaaaaca gatcgaagga gaaaaaatcg aacttctcga ttgggtgaat
    16201 gaaaatctgg aagaaactta cgttcatgag gcgaatgggg aacgtgaaga acaacgaaag
    16261 gatttttcaa gtgaataaat ttttaggatt tggagcagtt gtgattatga tcgcagcaat
    16321 gggctatgtt atttttagcg atgatgattc gcatgttcaa gccggcaatc aagcagtcga
    16381 acgaacaagc accaaaacaa ctgctagtaa agaaaagaac agtcaaacaa ccagcagcaa
    16441 ggtaaaggac aaagaaacaa aagatcgtac gtccaaacaa ttgccagatg ttaaaacaga
    16501 cgattggaag ctagttttgg ttggtccaga tcacaaatta gaaaaagaaa ttgatgagtc
    16561 tactcaattg gcatcattaa gtaacggata cttgatcgac aaacgcattg ctccagaata
    16621 tgaagcattt gccaaggctg ctgaagcagc tggatttcca ttagtgatgg tctcagctta
    16681 ccggtctgta tcatcccaac aacaagtttt cgaacaaaat gtccaagatg tgatgagtcg
    16741 tcaaggtgta tcagaagagg aggcgacaaa gattactaaa gaaacgatta cggagccagg
    16801 atacagcgaa caccacacag gtctggcagt cgatgtcgtc gatcaaaact ggtacaacag
    16861 ttatccttct actgtattgg atgcaagtta cggagatcag cctggagcta aatggatcgc
    16921 agataatgct gcgcagtatg gatttatcgt tcgttatcca aaaggacacg aagatataac
    16981 taagatcaca tatgaacctt ggcacttacg ttatgtcggt aaagaaaacg ctgagtatat
    17041 caccaaacac caattgacgc tagaagaata tctgaatgaa ttaaatgaaa agtaggaatt
    17101 gatcatggcg aaaaaaaagt ggaaaaagca aaagcgcaag aatatccgtt ggccagcagt
    17161 ggttgccggt gtagcagtga tactcttagc ctttgttttt tccttgaaga atctgtcttc
    17221 tccttttact gataatcagc aagaaaatga agcgcaatcc catcagcaat ttattgaacg
    17281 gcttgttccg catgcccaag aactacaaga tggttatgga gtgcttccta gtatcatttt
    17341 aggacaagct atattagaat caaattgggg aaaaagtacc ttggctagca aatacaataa
    17401 tcttttcggg atcaaggctt atggggatca gaagaaagta agcctggaaa caaaggaatt
    17461 tgttaacgaa gaatggataa ctatccaagg agacttcaaa gtgtatgatt catgggaaca
    17521 gtccatggat gaccatacac aactgttcgt ccaaggagta gattggaatc ctgcgctgta
    17581 tgaaaaagtg attacggcca ccaactatca ggaagccgca caagcattgc aagatgcagg
    17641 ttatgcgaca gaccccggtt atgcgcaaaa aatcatccag gtcatcgaga cgtatcaatt
    17701 gaatcagtat gaccggagat agcaaaggag aagtggctga tggcagaaca atttacgcca
    17761 gaaatcatga cggaattgag aaaagatatc gtaagagtac cggaaatcat tttagaagca
    17821 agcgggatca agatatttgg aaaattgatc cgttcgatca tctttactac ggatattgcg
    17881 atcatacgta atacaaatgc gaatgcagta attgctgtgt atccttttac acctcatcca
    17941 gcgattacta aaagcattat tgaggcagct gacattccag ttttctccgg tgtaggtggt
    18001 ggtttgacac aaggtgctcg ttcttcctac atgagcatgt tcgcagaagc gcaaggttcg
    18061 ttaggggtcg ttttgaacgg tcctacgcct ttagagacag taaaagaagt ttgtcgagta
    18121 gttgatattc cagttatcag tacggtaacg tcgaaatata cagaaataga tgaaaagtta
    18181 gcagctggta tttcgattat caatatcagt gcaggtaaag aaactgcaga agcagtacgc
    18241 cattttcgta aaaaatatcc tgaacttcct atcattgcga caggtggtcc aacggaagaa
    18301 agtattgctg agaccattca agcaggagca aatgccatca catatacgcc accaagtagc
    18361 ggagagcttt ttagtcgtaa aatggaaaaa tatcgggaaa ctgagaaaaa gaatcatgaa
    18421 taacaggtga gaatcaaaag acgaggaaga gcagatagtg gctcttcctc gtcttttgta
    18481 ctccagtact atttgttcag aattcaaaaa aaagagaaat ttttttgttt ttttacctgt
    18541 aatgtacata agaagagtta aaaccttttg ttattattag cattctaaca aaaaaatgtc
    18601 ttgatttatt gaagcgcttt ctatagaatg aaaaacgaat aaaaactaaa aaaggcaccc
    18661 gacaatttag tagcagctaa attgtcgggg cttgaaaagt cagcgggaac tgaccaatca
    18721 agttgccata gtcaatgctt gtgcatcgac gtgtatattg tacctaattt catacagaat
    18781 tactagttat ttttctgttt tttagggaag agcgcacacg aagaagcatg cttatttagc
    18841 tatgtcaagg atattgggca gttgtctgat atcctttttt ctttgcgtaa ttatcctccc
    18901 atcattgaag caagaagtgt ttttagcgat gaaagaagaa tcagcactat ctaatttttc
    18961 ttgaaagtat tgagttgttc atcctaggca gaacgataag gcaagtcttg gtttttattc
    19021 cccttgaaaa agatcgttta ataaatatgt tctgtataaa aatacagtat attgaaaaat
    19081 aacaatggaa gtagaggtgc agagaatgaa gaaaagaatg agaatcatct tagctttaag
    19141 tgttatgtta ttcttcttga cggcttgtgg gcaagaagat gacaaaaaaa tcacgctgaa
    19201 catggcttgg tggggttcgc aagtccgaca cgatgcaaca gtaaaagtaa tcgagcttta
    19261 tgaagaaaaa aatccccatg tgacgatcga ttatgaattt tatgattatg aaggatatta
    19321 cacaaaatta ggtacactag cagcatcaga tgatatctgg gacattttcc aactgaacaa
    19381 tcaatttcct caatacatca atcagattga accattagat gaatatgtgg agcaagggat
    19441 aattgatatt tctaatgcag atgaatccta tttagcaacg acgacttacg acgggcaatt
    19501 agttggactg tctaacgggg tgaattcgtt tgcgattgct tacaataaaa atatatttga
    19561 acaattaaat atccctgaac cagcttcaaa ttggacatgg gatgaatttg aacaaatcag
    19621 ctatcaaatc actgaagaat taggaatcta tgctatttca agattcgaag attttatagc
    19681 tggctgtatt attcaaatac cacaagttga gaaaggattg aatttttata atcgggaaga
    19741 tctatctaac agcttgggat ttgaaaatcc agactatcta acagattttt tccagctgcg
    19801 taaaaatctg gtagacaaag gggcccatcc agaaccaggt ggtggagctg caagtacgac
    19861 ggatctgtct tttcaggcgg ttagggatag agaagcggca atggtgtttc tatcaagtaa
    19921 ccagttgaca gaaatattgt caggggctcc tgaagatatg gagattcagt tgattaatcc
    19981 acctcgcaga aaagcagatg gagaatctgg tatcccttta cgttcttcgc aaatgctgag
    20041 tatggcaaaa agctcagccc ataaagaaga agctgctaag tttattgatt tctttcaaaa
    20101 cagtgaagaa gccaatgaaa ttttaaaagg agaacgtggt gtaccaatca tgtcgaatat
    20161 tcgagaacag ctcgctcgtg cagatgatgc aactttgcaa gcgacatttg attatttaga
    20221 aatgatttcc gatatggaca acggcgaagt gaatgtcttt gatagtgctt tgaatccgga
    20281 aattcaagac caatacaatc ttttagttga aaaagtcatt tatgaagaag taacagcaga
    20341 aggaaggcat gaaaaaagaa aaactaaaac ggttcttgga tcaagacaat gtggtcggtt
    20401 acgtatttgc ttctccattc atcctaggat ttctattgat aacagtgata ccgatgatct
    20461 tgtcacttta ttattcgttc accgattatc gtttgggaaa tccagtctca tggatcggct
    20521 tggataatta tataaagatg ctgagtgatg cccgtttttt gaactccatc cgagtcactt
    20581 ttctctatgt atttgcatct gtacctgtga aactgatctt tggtctattg attgcttatc
    20641 ttttaacgag gaatatcaaa ggaacgactt tctttcgttc tttgtattat gtgcctagtt
    20701 tgatcggtgg cagtatttct atcgctctag tttggcggga gttgttttct aaaaacgggt
    20761 tagtgaatat tttcctggaa cgattaggcg cacctgcgct ttcctggttt agcgatcaaa
    20821 aactggtcat gatccctctt gttttgatgt ctgcttggca attcggttcg gcaatgatca
    20881 tctttgctgc aggtatcaaa gagatcccag gctcgtatta tgaagcggca gagatcgatg
    20941 gagcgaacaa atggcagacg ttttacaaga tcacgttacc tcttctttcg ccgatcattt
    21001 tgtataactt ggttatgcag acaatcgcgg cgtttatgac ttttacgcag gcatttatca
    21061 ttacccaagg tggtccgaat gatgccacga acttttatgc tctttatgtg tataaccaag
    21121 ctttttcttt taattctatg gggtatgcta gtgctatttc gtggatcatg ctgttgatta
    21181 tgggagctgt cacctttctg attttcaaat catcaaaaaa atgggtcgtt gtgtgaatga
    21241 gaggaggaag aaatgaataa tacagtaacg aaatggtttc aatacggttt tattttgcta
    21301 ttaggtctag tcatgattta tccggtctta tggatgatcg caggttcttt taaaacggat
    21361 caagagatta tgagtggttc gctgaatttg attccagaga cttttcaatg ggaaaattac
    21421 gcaaaaggat gggcggggtt tgctggaatt tcatttttta ctttctttag gaattcgttt
    21481 gtcatctctg cagtttcgac gatagggaca gttctgagct cgacttgtat tgcttacgca
    21541 ttatcgagaa tcgatttcaa aggaaaaaga ttctggttcg tcgtcatgtt agtaacgatg
    21601 atgattcctg cacaagtgat tttgattccg cagtttatta tttacaatcg gctgaatttg
    21661 gttgggacgt atgtgccgct gattttacca catttttttg gacaagcatt tttcatttac
    21721 cagatcatgc aattcatggt cagcatcccc aaagagttag atgaatcagc gattattgac
    21781 ggatgcagca agtatagtgt ctttaccaaa attatttttc ctttgctgaa accttcgatc
    21841 atcacgacga tcatcatcca gttttattgg aaatgggacg acttcatggg accattgatt
    21901 tatttaaaca agccgagatc ttacacggta tcgattgcca tcaaattatt tgcagacgct
    21961 ggatctagta cgacttacgc gagtatgttt gcgatgtcta cgttgtcgct gctaccggtt
    22021 tttctgattt tcttattctt caataaatat cttgtccagg ggatcagtac aagcggtctg
    22081 aagggctgaa tatgaaaaag ccaatagaag agatgtttct ttacaagctc aactactatg
    22141 tctgtcggat ttttttaacc agtcttatca gtaatgctct tttcgcttta gttctaggat
    22201 gtttgttcac tttaggatta gtgctgtctg ctcgtgttgt ctatctgatt tattttttga
    22261 tgatgtatcc ttcttattat ctatctgtcc attacttggc agaaaaaaag gtatgttgga
    22321 ggaaagggaa aatcaaacgg atgtgtttca tcattacgtt gtttcagctg tttgtcatta
    22381 tttttgcagt tgattggtat ttattagtgg gaaagattgg agcttggtat ctgattcctt
    22441 ttctattgtg tctgttgctt tttgtatccc atagtttttt tagtacgcta attgatatct
    22501 gcaaagaaga taaagcagat agtcttgttt atttgatcaa aaggaatatc tggctgttgt
    22561 caaaaaacgt caaggggttt ctgtggtatt tatgcctttt gtatggactg ttctggtgta
    22621 tacagcctga ttttttactc actgcattac tgtatccagg gctgccattt gtcacagtat
    22681 tttgcttaaa agaaaaggag aatgacttta aatgaagaaa aaatatgtac aagtcggact
    22741 tggtgggaga gcgcgttttt tttatgaggc aattgcctct acgtatcatc aaacttgtga
    22801 actagtcggt ttttgtgatg tatctcaaac aaggatggat tatgcaaatc ggatacttgt
    22861 gaacaagtat ggacagaaaa aagtaaagac atgtatgcca tcacaatttg agcaactact
    22921 ggatgacgta cggccggctg aagtcattat tacgtcagta gatcgaacgc atcatgatta
    22981 tatcattcgt gcaatgaaaa aaggatatga tgttatttgc gaaaaacctc tgactatcga
    23041 tgaggaaaaa gctcaagaaa taataacggt ccaaaaacag acgggaagaa gcctgcgagt
    23101 cacttttaat tatcgttatg caccgtatca cacgaagata aaggaactga tagaaagtga
    23161 tgtgattggt gatgtgtttt ctatccactt cgaatggctg ttaaatacgc aacacggggc
    23221 agattatttc agaagatggc atcgaaataa gcacaatagc ggcgggcttc ttgttcataa
    23281 atctacccat cattttgatc tagtgaattt ttggttgaac agccgtcccc aaaaggtatt
    23341 tgcattcggt ggattaaact tttatgggat gcataatgcg gagaaacgcg gagtagaaat
    23401 attttacagt cgatgctatg gaagcgaact agctaaagct gatccatttg ctatagacct
    23461 gtcagccaat gaggaattgc gagaaatgta tttagaagca gaaaaagaca gtggatatat
    23521 tcgtgaccgc agtgtatttt ccgatgatat ctctattgaa gatacactag gcgtaacggt
    23581 caaatatgaa actggtgcga ttttgacgta ttcgctaaac gcgtatatgc cgtgggaagg
    23641 attcaatgtt tctttcaatg gcagcaaagg acggatcgaa atgaaagtga tcgaaaagtc
    23701 ttacatcaat gcaggtggag ataaaaagga tgaaggagca gccgttcttc agtccatcca
    23761 tgtctatccg atgttcggtg agccatatga ggtaatcgtc gaacaagaag cgggcggaca
    23821 tggcggagga gatccgcgtt tattagatga tctatttggt gaagaaaaag aagaagatcc
    23881 gtggaatcgg gcagcaactc acgtagatgg tattttgtcg attttgacag gaatcgcagc
    23941 aaatcattct attgcttcag gaaaagcagt agtgattgac gagcttgtca gcttaaaaga
    24001 ataagaatag atttacggaa attacttccc gtagtttata aaatcttgcc ttattgccga
    24061 taggctgtct tttgaacact atttattcgg aaaaaggggc tatgacatgc tttttgtcat
    24121 gccttctttt tatgtgggct gcttttttag ataggagaaa ataaaaataa ctatttaaac
    24181 ccgaacaaaa aggaataatg cattaaaaat cattagattt tacaaaaaaa taagtaaacc
    24241 acgttgtcag tttcagaaag tagtgttaga ctgttttcaa tatttgaaac aacatttttt
    24301 attcaaatat ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg aatttacact
    24361 attattagtt ttacatacct acttatatat cgtcataata tggatgacag tttctagcca
    24421 gtaccgtaaa tgctggacta taagtaaaag attggctatt tcacccttct tttcttatga
    24481 taaagtggag ggtgtttttc tttaacagaa acatagtagg tttttgcaca aaagcggtta
    24541 tttcggtatt aataaatgat aaaggagcaa ataaagtgaa agaattagtt gaacgtattc
    24601 aaaaagacgg acgtatatta ggcgagggcg tattaaaagt cgacagcttc gtcactcacc
    24661 aagtagaccc tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac
    24721 aaggaatcac gaaagttgtg accattgaag cttctgggat cgcaccagct ttatttgctg
    24781 caagagaatt agatgttcca atgatcttcg ctagaaaagc aaaaagtttg acgatggatg
    24841 aagaattatt gacagcttct gtttattctt ttacgaaaca agtgacaagt acgatttcta
    24901 tttctcgtaa atttttatct tcagaagaca aagtattgat tatcgacgat ttcttggcaa
    24961 acggacaagc ggcaaaagga ttgatcgaat tatgccagca agctggagcg caagtagaag
    25021 gaatcgggat cgtcatcgaa aagtctttcc aagatggacg tcagttactt gaagaaatgg
    25081 gattgagagt cgtttctctt gcgcgtattg cttccttgac gaatggacaa gttgagtttc
    25141 ttgaggagga cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg cggttttagg
    25201 gctgcagcat ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac
    25261 aggactaggc tttaatcaag aacagatgac ttatttgatt tcaattgata tctttatgtg
    25321 tggattagcg acattattgc aattgacagt taaccgattt ttcggaattg gcttgcctgt
    25381 cgttttagga tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa ccaatgaagg
    25441 agtaggagct atctacggat ctattatcgc ttctgggatc tttgttgtgt tagtttctgg
    25501 tgtcttttct aaaattaaaa aactattccc tcctatagta acaggaacgg ttatcaccgt
    25561 tattggtttg actttgattc ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc
    25621 agctgatttt ggtgacaaaa caaatctgct acttgcattt gtgacgattc tcctaattgt
    25681 aggcgtgcaa atgctggcaa aagggttcat tcgttcaatc gcggtattga ttggtttagt
    25741 cggaggaagc atcttagctg ctttcatggg aatgattgat ctgagcgcga ttggagatgc
    25801 gccgttgttc catgttccac aaccctttta ttttggtaaa ccaactttcg acgtatggtc
    25861 gattttacta atgatcatca tttccatggt cagcatggta gaatcaacag gtgtatattt
    25921 tgcacttggt gacattactg ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg
    25981 tgcagaagga cttgccgtta tcttaggcgg tattttcaat acattccctt atactggctt
    26041 ttctcaaaat gtcggattgg ttcaactttc gggaatcaaa acaagaaaac ccatctattt
    26101 ctctgcattc ttcttgattg ttttaggatt attccctaaa atcggtgcgt tagcacaaat
    26161 catccctgag ccggtacttg gtggaggaat gttagtcatg ttcgggatgg tggcagtcca
    26221 aggaatccgt atgctgttgg aagttgattt cagcaatgat aaaaatctat tgattgttgc
    26281 tgtttctgtt ggatttgggc tgggtttcaa tgttacgccg acattattca gtcaaatgcc
    26341 gcaaacattg caaatgttca ctggaaatgg catcgtaatg agtagcatta cggctatcat
    26401 attgaatttg atctttaatg gattgaaaaa agatgaagaa atattggaag aagccttcaa
    26461 gaagtaatag agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt gttagagaaa
    26521 caaacggaaa aatagttcct cgtattttgc cgtttgtttg agctaatacc gagggtcagc
    26581 tgattgaaca ccgtttatcc ataaaaagga atgtgaccta acttttgtca cattcctttt
    26641 tttcatcttt aataaacacg aacaataaaa tgtttttctt tgttaacatt cgaaatttct
    26701 ttgacatcaa aaaaagtgat tgttaaaata tgaatgtaga aaaactggga ggaaaaacaa
    26761 tgacagcaaa aatctctgtg attatgggga gtacttcaga ttgggaaaca atgaaagaag
    26821 catgtcaaat attggatgaa ttcggtgtgt cttatgaaaa aaaggtcgtt tctgctcatc
    26881 gaaccccaga tttgatgttt gaatatgcac aaaacgctcg aaaaaaagga atcaaaatca
    26941 ttattgcagg agcaggggga gccgcgcatc tgccgggtat ggtcgcatca aaaacaactt
    27001 tgccggttat cggtgtaccg gtccaatcac gaacattaaa tggtttggat tcgcttcttt
    27061 cgattgtcca aatgcctgga ggagtacctg tagctactac agcaatcgga aaagcgggag
    27121 caattaatgc tggactactt gcgatacaga tgctttccat gtatgattta gaattggagg
    27181 ctaaattggc tgaaagacgt tcaatgttgg ctaaaactgt tatagaaagt agtgatcaac
    27241 ttggttaaac ctttgttgcc tggttcaacg atcgggatcg tcggtggcgg tcaattaggt
    27301 cgtatgatgg cgattagtgc aaaagaaatg ggctttaaag taggtgtgct ggatcctgtg
    27361 agcgattgtc cagccgcaca agttgcagat tggcacattg aaagtgtcta tgatgacacc
    27421 tttgcgctga aagagcttgc cagaagaaca gatgtcatta cctatgaatt cgaaaacgtc
    27481 agtgtagagg cgttaaatgc gattttaccc atgtcattta ttcctcaagg gacagacttg
    27541 cttgcgatta cgcaagaccg cctgttagaa aaatcttttt tggaaacgaa taacatcgtg
    27601 attgctcctt atgcaacgat cgtcagtccg acggatatcc aagatgcgat tgacgggatc
    27661 ggctatcctt gtgttttgaa aaccactcga ggcggctatg atggcaaggg gcagtatgtt
    27721 ttgaaaagtc gggcggatct agctcctgcc atggaccttt taagagaagg aacctgtgtt
    27781 ttagaagcat ggatcccgtt tgaaaaagag atttcgatca tggtggcagg aaatggtcaa
    27841 ggagatttca cgacttttcc ggtcgtggaa aacatccacc acaacaatat tcttcatgaa
    27901 acgattgctc cagccgcgat tgatcaggat gtgatcgagg aggctgagcg tattgcccgt
    27961 gtgattgcag aagcagtgag cctttcaggt gttttagggg tagaaatgtt tttaacaaaa
    28021 acaggcgggc tatatgtgaa tgaattagct cctcgtcctc acaattctgg acattattct
    28081 attgaagctt gttctatgag tcagttcgat gcccatattc gaggtatttg tggttggcca
    28141 ttaggagaaa aagtacgttt gataagcaat gcagtaatgg tcaatgtttt gggagaacaa
    28201 ctgctggaaa gctatcgctt gattgagaat aagccagact ggcaattcca tttctatgga
    28261 aaaaaacaag caaaaaaagg tcgcaagatg ggacatatca ctatcttgac agaagacatc
    28321 cagcaaacgc tggaagaaat caaacaaacg aatatttggg attagaggag aaaaaacatg
    28381 ttagaacgtt atacacgccc tgaaatgggg aatatctgga cggaagagaa caaataccgt
    28441 gcgtggctcg aagtcgagat tttagcagat gaagcttggg cagaacttgg tgaaatcccg
    28501 caagaagatg tgaaaaaaat tcgtgagcat gcatcatttg atatcgatcg gatcttggaa
    28561 atcgaaaaat taacacgcca tgatgtcgtt gcgtttacac gagcggtttc tgaaacattg
    28621 ggagaagaaa gaaaatgggt ccattatggt ctaacaagta cggatgtcgt agatactgct
    28681 tatggctatc tcatcaagca agcaaatgat attttacggg aagacttgaa acgttttacg
    28741 gaaatcattg gcgaaaaagc aaaagaacac aaatatacag tgatgatggg tcggacacac
    28801 ggtgtccatg ctgaaccaac tacttttgga ttgaaacttg ccacatggta ttcagaaatg
    28861 aaacggaaca tcgaacgatt tgaacatgcg gcaaaaggag tagaagcagg aaaaatctca
    28921 ggagcagtgg gaacgttcgc aaatatcccg ccatttattg aagagtatgt atgtaaacaa
    28981 ttggggatcc gtccacaaga aatctctact caagttctcc ctcgggatct acatgctgaa
    29041 tatttctcag cgatggcgct tattgctaca agtattgaac gttttgcaac agagatacgc
    29101 ggattgcaaa aatccgaaac acgtgaagta gaagaatttt ttgcgaaagg tcaaaaaggt
    29161 tcttctgcta tgccgcacaa aaggaatccg atcggttcag aaaatatggc tggtctagcc
    29221 agagtggtgc gcggacacat ggtcactgcc tatgaaaatg tcacattatg gcatgaaaga
    29281 gatatttctc attcttcagc agaacgaatc atcttgcctg ataccacgat tttattgaat
    29341 tacatgttaa atcgttttgg caatatcgtt aaaaatctaa cggtctttcc tgaaaacatg
    29401 aagcggaata tgtctgcaac atttggtttg atttatagcc agcgcgttat gctgaaattg
    29461 atcgataagg gaatgacaag agaagaagct tatgatcttg ttcagccaaa aactgcgtac
    29521 gcttgggaca accaagtgca gttccgcccg ctgttagaag aagatccaac catcacatcg
    29581 gtattatcta aagaagaaat agacgatgcc tttgattatc atcatcattt gaaaaacgtt
    29641 gacgtgatat tcaaacgagt aggtttggaa taaagaagag tgagttgcaa aattcaatta
    29701 aaatagaata gcaaaaataa ccaaaaaaga agcagatccg ttagcggttc tgcttctttt
    29761 agtttattat attttcttat caataatagg aacctgattt taaaagcggc tacctcttta
    29821 ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg gctattataa
    29881 ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta ttccatctct
    29941 tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg ttcatgaatc
    30001 tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct tttacggcat
    30061 tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact agtatcttta
    30121 ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga acgttcttca
    30181 gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat cggcaagtgc
    30241 ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat taagtataaa
    30301 tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga
    30361 atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt
    30421 aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga
    30481 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
    30541 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
    30601 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
    30661 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
    30721 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
    30781 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
    30841 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
    30901 tcttacaact tgcaaaaccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
    30961 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
    31021 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
    31081 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
    31141 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
    31201 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
    31261 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
    31321 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
    31381 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
    31441 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
    31501 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
    31561 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
    31621 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
    31681 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
    31741 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
    31801 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
    31861 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
    31921 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
    31981 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
    32041 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
    32101 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
    32161 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
    32221 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
    32281 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
    32341 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
    32401 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
    32461 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
    32521 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
    32581 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
    32641 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
    32701 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
    32761 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
    32821 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
    32881 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
    32941 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
    33001 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
    33061 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
    33121 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
    33181 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
    33241 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
    33301 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
    33361 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
    33421 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
    33481 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
    33541 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
    33601 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
    33661 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
    33721 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
    33781 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
    33841 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
    33901 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
    33961 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
    34021 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
    34081 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
    34141 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
    34201 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
    34261 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
    34321 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
    34381 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
    34441 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
    34501 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
    34561 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
    34621 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
    34681 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
    34741 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
    34801 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
    34861 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
    34921 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
    34981 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
    35041 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
    35101 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
    35161 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
    35221 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
    35281 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
    35341 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
    35401 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
    35461 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
    35521 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
    35581 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
    35641 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
    35701 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
    35761 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
    35821 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
    35881 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
    35941 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
    36001 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
    36061 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
    36121 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
    36181 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
    36241 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
    36301 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
    36361 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
    36421 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
    36481 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
    36541 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
    36601 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
    36661 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
    36721 tcggtatggt cctggcagtc gaagaatcga aagtaaaaga aatagtacac gaaattgagc
    36781 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
    36841 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
    36901 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
    36961 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
    37021 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
    37081 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
    37141 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
    37201 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
    37261 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
    37321 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
    37381 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
    37441 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
    37501 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
    37561 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
    37621 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
    37681 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
    37741 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
    37801 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
    37861 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
    37921 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
    37981 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
    38041 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
    38101 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
    38161 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
    38221 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
    38281 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
    38341 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
    38401 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
    38461 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
    38521 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
    38581 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
    38641 gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
    38701 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
    38761 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
    38821 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
    38881 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
    38941 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
    39001 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
    39061 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
    39121 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
    39181 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
    39241 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
    39301 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
    39361 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
    39421 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
    39481 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
    39541 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
    39601 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
    39661 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
    39721 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
    39781 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
    39841 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
    39901 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
    39961 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
    40021 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
    40081 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
    40141 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
    40201 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
    40261 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
    40321 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
    40381 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
    40441 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    40501 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    40561 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    40621 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    40681 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    40741 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    40801 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    40861 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    40921 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    40981 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    41041 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    41101 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    41161 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    41221 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    41281 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    41341 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    41401 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    41461 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    41521 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    41581 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    41641 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    41701 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    41761 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    41821 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    41881 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    41941 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    42001 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    42061 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    42121 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    42181 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    42241 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    42301 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    42361 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    42421 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    42481 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    42541 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    42601 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    42661 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    42721 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    42781 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    42841 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    42901 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    42961 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    43021 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    43081 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    43141 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    43201 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    43261 catgcggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    43321 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    43381 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    43441 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    43501 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    43561 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    43621 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    43681 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    43741 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    43801 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    43861 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    43921 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    43981 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata
    44041 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    44101 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    44161 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    44221 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    44281 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    44341 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    44401 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    44461 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    44521 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    44581 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    44641 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    44701 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    44761 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    44821 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    44881 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    44941 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    45001 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    45061 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    45121 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    45181 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    45241 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    45301 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    45361 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    45421 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    45481 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    45541 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    45601 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    45661 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    45721 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    45781 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    45841 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    45901 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    45961 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    46021 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    46081 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    46141 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    46201 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    46261 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    46321 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    46381 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    46441 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    46501 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    46561 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    46621 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    46681 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    46741 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    46801 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    46861 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    46921 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    46981 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    47041 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    47101 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    47161 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    47221 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    47281 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    47341 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    47401 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    47461 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    47521 gcgaagagca tattattcgc gggatggtgg gcagagattt agttgatcgt tatcctgaaa
    47581 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    47641 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    47701 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    47761 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    47821 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    47881 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    47941 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    48001 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    48061 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    48121 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    48181 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    48241 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    48301 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    48361 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    48421 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    48481 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    48541 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    48601 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    48661 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    48721 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    48781 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    48841 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    48901 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    48961 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    49021 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    49081 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    49141 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    49201 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    49261 atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca
    49321 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    49381 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    49441 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    49501 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    49561 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    49621 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    49681 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    49741 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    49801 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    49861 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    49921 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    49981 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    50041 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    50101 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    50161 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    50221 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    50281 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    50341 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    50401 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    50461 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    50521 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    50581 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    50641 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    50701 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    50761 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    50821 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    50881 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    50941 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    51001 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    51061 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    51121 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    51181 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    51241 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    51301 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    51361 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    51421 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    51481 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    51541 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    51601 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    51661 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    51721 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    51781 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    51841 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    51901 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    51961 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    52021 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    52081 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    52141 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    52201 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    52261 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    52321 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    52381 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    52441 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    52501 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    52561 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    52621 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga
    52681 tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaaaataaaa cgctttcctt
    52741 attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt
    52801 gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac
    52861 ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta
    52921 tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc
    52981 actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa
    53041 cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac
    53101 tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg
    53161 cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc
    53221 atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca
    53281 tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta
    53341 tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc
    53401 agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac
    53461 tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta
    53521 ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga
    53581 aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat
    53641 tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg
    53701 ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca
    53761 tacactatct ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca
    53821 gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga
    53881 agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga
    53941 agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc
    54001 tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg
    54061 gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac
    54121 gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact
    54181 gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct
    54241 ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc
    54301 caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca
    54361 agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc
    54421 ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct
    54481 ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct
    54541 attagaagta acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga
    54601 gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag
    54661 ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca
    54721 gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca
    54781 ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa
    54841 gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct
    54901 atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt
    54961 ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa
    55021 atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaattg
    55081 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    55141 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    55201 atcccgtcac ctgttacagg aacggttaaa aatgtgattg ttccagaagg taccgtagca
    55261 aatgttgggg atgtattagt agaaatcgat gcaccaggac acgaagacaa tgaaggggac
    55321 agtggtgtag ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc cactgttgat
    55381 acagaatcag ctggatcgtc aagcgaagga gtcttccaat ttaagttacc ggatatcggt
    55441 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
    55501 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat tccgtcacct
    55561 gtcacaggga cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat
    55621 gtattagtag aaatcgatgc tcctggacac aatagtgcac cgtcaactag tgcgccatca
    55681 gctgaagcac caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc
    55741 gatccaaata aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat
    55801 gtagatatct cacaagtaac tgcaactgga aaaggtggca gagtaacaaa agaggacatc
    55861 gaaaacttct tggcaggtgg tccaagcagt gcaccagcga aatcagaagc gccagaagcg
    55921 gcagcaccga aagaagcagc tcctgcagca gaaagcaaac cagctgcacc agcaaaacca
    55981 ttcaagtcca acttaggaga cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct
    56041 atcgctaaag caatggtcaa cagtaagcat acagctccac acgtgacatt gcatgatgaa
    56101 gtagaagtgt ctaaactatg ggataatcgg aaacgattta aagaagttgc tgctgcaaat
    56161 ggtacgaaac ttactttctt gccatatgtc gtgaaagctt taacagcgac tgttaagaaa
    56221 tacccagtat tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta taaacattac
    56281 tacaatatcg gtattgcaac tgatacggat cacggactat atgtaccaaa tgtgaaagat
    56341 gcagaccgca aaggaatgtt tgctattgca gacgaaatca atgagaaagc aaaattagct
    56401 catgacggca aactatctgc agaagatatg cgcaatggaa caattacgat cagcaatatc
    56461 ggatctgtag gtggcggttg gtttacacca gtcatcaact atccagaagt agctatttta
    56521 ggtgtaggaa cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt
    56581 cgtgtgatga aactgtcatt aagtttcgat caccgtatcg tagatggagc aactgcgcaa
    56641 caagcaatga ataatatcaa acgtctttta gctgatccag agctattaat gatggaagga
    56701 tgataaacaa atggtagttg gagattttgc tgtagaatta gatacagttg taattggtgc
    56761 gggacctggt ggctatgtag ctgccatccg cgctgctgaa atgggacaaa aagtagcaat
    56821 cattgaaaga gagtatattg gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc
    56881 actgatagct gcaggacacc attatcaaga gtcactagat tctgaaatgt ttggagtaac
    56941 tagtgaaaac gtcaaactag attttgcaaa aacacaagaa tggaaagaaa acaaagttgt
    57001 ccatacgttg acttctggtg ttggtttcct attaaaaaaa cataaggttg aaacaatcga
    57061 aggagaagcg ttcttcgtag atgatcacac actacgcgtc attcatccag attcagctca
    57121 aacttattcg ttcaataacg caatcatcgc aacaggttcc cgtccaatcg agattccagg
    57181 atttaaattc ggaggacgtg tgttagattc tactggcgga ttagcattga aagaagtacc
    57241 taagaaattt gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc
    57301 aaacttagga gcagaagtaa cgattcttga aggctcacca caaattttac caacttacga
    57361 aaaagatcta gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac
    57421 gaatgcaatg gcgaaagaag cagtcgataa cggtgacagc gtgacagtga aatatgcagt
    57481 ggacgggaaa gaagaatccg ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc
    57541 gaatacagat gatatgggct tagaacaagc gggtgtcgaa gtaggagaac gaggattgat
    57601 cacggtggat aaacaaggac gtacgaacgt acctaacatc tatgccatcg gtgatatcgt
    57661 tccaggggcg gcattagcac acaaagccag ttatgaagca aaaatcgctg ctgaagcaat
    57721 ctctggcaaa aaagtagctg tagattataa agcaatgccg gcagtagcct tcactgatcc
    57781 tgaattggct tctgtaggta tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa
    57841 agcatataaa ttcccatttt cagggaacgg tcgggcatta tcgcttggta aaacagaagg
    57901 attcatccgt ctagttacga cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg
    57961 agtcggcgca agtgatatgg tctctgaatt agctttagca atcgaatcag gaatgaatgc
    58021 tgaagatatc gccttgacga ttcatcctca tccatctctt ggcgaaatcg tcatggatgc
    58081 ttcggaatta gcattaggtt taccaatcca tatttgattt gaataataca ggcgaaacgc
    58141 aactgaaaaa tcagttgcgt tttgtttttt agaattatca ataatattat tatgtaaact
    58201 taaaaatatg acaacttctt ggtttattac cacttcctat gctacactga agaggaatat
    58261 gaaataggag agttgaagat gcaagaatat cagtatccac ttgatttaga ttggacaaca
    58321 gacgaaatgg tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg
    58381 ataaacaatg aagctttttt gaagacttat cagcagttta aaactgttgt caaatccatt
    58441 ggtgaggaaa aacgattggg acgggaattt gaaaaagctt ccggttattc tttatatcgt
    58501 acagtgcagc aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg gtgagataaa
    58561 tggaacagat gattgatcat atcaagtcct ggcttgaaga agctggtgag aaaatcaaat
    58621 cttctcaaca aacattaaca atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg
    58681 tcgatcgaaa catccagaat tttctaatcg aaaaaatcca ttcctttgat ccagaggcga
    58741 aaatattggc agaagaagaa gggtgtaatc aattatcgga tctcgatgga agagtcttta
    58801 tcatcgatcc tattgatggc accttgaatt ttgtgattga aggggaaaat ttttgtatta
    58861 tgctcgctgt ttatgaatcc ggcgttggaa agcttggttt catttatgac gtgatgagaa
    58921 atgaactttt ttggggtgga aaaggaatag gcgtgtataa aaatgatgag ccattgccta
    58981 gaccagcaaa taaatcctta tcggaaggct tacttggtgt gaatagttat cttttcggtc
    59041 ataataaata tggaatccga gaaattgcgg aaagcagcat ggggacacgt atgtacggat
    59101 gcgctggtct tgaactagtg gcaatcttga aaggaaacca cattgcttat atttcaaacc
    59161 ttagcccttg ggattatgca gcgggaaatg tcttattgga agagtttggt atggtaacaa
    59221 gcggtctttt tggtactcct ttgacatttg aaaaaaggga atactattta gcagggactt
    59281 cttgtgcata tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa
    59341 atcattcaca aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca
    59401 ttttactata tttcatgaaa aaagtctgct ataattagca gtcgagtaaa atttgccctg
    59461 tcataaagaa ccaataaaaa aggagttagt tacattgaat tatagaaacg atatccgtaa
    59521 cgtcgcaatt attgcccacg ttgaccacgg aaaaacaaca ttagttgatg aactattaaa
    59581 acaatcagat acattagacg cacatacgca attgcaagaa cgtgcgatgg actcaaatgc
    59641 gctagaaaaa gaacgtggta tcacgatttt agccaaaaac acagctgtag actataaagg
    59701 tattcgcgta aacatcatgg atactccagg acacgcggac ttcggtggcg aagtagaacg
    59761 gatcatgaaa atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc
    59821 tcaaacacgt tttgtattga aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt
    59881 aaacaagatt gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact
    59941 attcatcgaa ttaggagcag atgatgatca gttagacttt cctgtgattt atgcatctgc
    60001 actaaatggt acgtcaagct tgtcagatga tccagcagat caagaaccaa ccatggcacc
    60061 tatttttgat acaattattg aaaaaatccc tgctcctgtc gataacagcg acgaaccatt
    60121 acaattccaa gtttcattac ttgactacaa cgattatgta ggtcgtatcg gaatcggacg
    60181 tgtatttaga ggaacaatca aagtaggaga ccaagtagca ttaattaaat tagacggaac
    60241 agttaagaaa ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac gtctagagat
    60301 tcaagaagca aaagctggag acttgattgc tgtctcaggt atggaagata tctttgtcgg
    60361 tgaaacagtt actcctgtgg atcatcaaga tgcgttacct atccttcaca ttgacgaacc
    60421 aacattgcaa atgactttct tagttaataa ttcaccattt gccggtcgag aaggtaaatt
    60481 cgtaaccgct cgtaagattg aagaacgttt gatggcagaa cttcaaacag atgtatcctt
    60541 acgagttgaa ccaacaaact ctccagatgc atggacagtt tctggtcgtg gagaattgca
    60601 tttgtctatc ttgattgaaa acatgcgccg tgagggatac gaattgcaag tatctcgtcc
    60661 agaagttatt gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat
    60721 cgatacacca gaagaatata tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga
    60781 aatgcaagat atggtccata caggaaacgg acaaatccgt ttgacattct taacacctgc
    60841 ccgcggtttg attggttatt caactgaatt cctttcaatg acacgtggtt acggaatcat
    60901 gaaccatact tttgaccaat atctgccaat gcttcctggt caaattggcg gacgtcacca
    60961 aggggcgctt gtttcgatcg atacaggaaa agcaacgact tattctatca tgagtatcga
    61021 agaacgcggt acagtcttcg tagaaccagg tacagaagta tatgaaggaa tgattatcgg
    61081 ggaaaacagc cgtgataatg atttgactgt caacatcacg aaagcaaaac aaatgacaaa
    61141 cgtacggtct gctactaaag accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt
    61201 ggaagaatct ttggaattct tgaatgatga tgaatattgc gaagtaacac cagaaagcat
    61261 tcgcttgaga aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa
    61321 ataatctgta aaaggatgaa gagagaaacc agcttgttat aaaaaatagc tggtttcttt
    61381 ttttgtccaa ctcttttctg tggagaagtc atctttgtca atggacataa attcagaaaa
    61441 tacgccgaaa gacgtttaaa agacttttaa aaaacttatt tctagagagt ttttgtttca
    61501 taataagata atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa
    61561 atggcaagag aaaggagcgg gaagatgaac gacttattaa aacaatactt tggttatgat
    61621 gagtttagac ctggtcaaaa agaaattatc cagaaagtca ttgatcagga aaatgtatta
    61681 ggaatcatgc ctaccggaag tgggaaatca atctgttacc agcttccagc tttactatta
    61741 gatgggctta ctgtggttgt gtccccttta atctctttga tgaaggatca ggtggacgcg
    61801 gcgaatcaat taggtatacc cgccactttt atcaatagct cgttagatgg ttacgaaaca
    61861 gctagacgct tccaagaaat cgatagacag cagtaccgtt tactttatat cgctcctgaa
    61921 cgttttatca tgccggattt tatccaagca atgaagcgat ggaacgtccg tatgatcgct
    61981 attgacgagg cacattgtat ctctcagtgg gggcatgatt tccgcccaag ttatttacag
    62041 atggcgaacc agctagatca actgccaaat cgtcctgtaa ttgttgcttt gacagctaca
    62101 gcgactgtgc aagtagctgc tgacataaaa agacttttaa aaattccaga aaacaaccat
    62161 attcagactg gttttgaacg agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa
    62221 gaacaatatc taatcgaata tttaaagatc aacaaaaatc aatctggtat catttatgca
    62281 gcaacgagaa aagaagtaga ccgtctctat catttgctta aaaaattcga tttttccgtg
    62341 ggtagatatc atggtggtct aaatgagaat gagcgaacag aaatgcaaga agccttttta
    62401 tacgatcgct tgcagctgat tgtagcgaca aatgcatttg gtatgggtat aaataaaagc
    62461 aatgtccgtt ttgttatcca ttatcaaatt cctggctctc tagaagcata ctatcaggaa
    62521 gctggtcggg cgggaagaga tggtctttcc agtgaagcca tcctgctttt ctctcctcaa
    62581 gatatccaag tccaaaagtt ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa
    62641 aaagaatatg aaaaattaaa agcgatgaca gaatacgtgt atattgagtc ctgtcttcag
    62701 caatacatcc taaactattt tggcgagaca agcagtccgt gcaaccgttg tggtaactgt
    62761 ttagatgaca gagaaatagt agaagtaacg acagaagcac agatggttct ttcttgctta
    62821 aaacgtatgg gtgagaacta tggtaaacaa atgctgatga aagtcttagc aggatcgaaa
    62881 gaacaaaagc tgcaagcttt aggttttgga catttatcta cctatggact gatgaaaaac
    62941 caatcgcaaa aagaaacgat gcagttaatt gaatatttga tatcgaatgg ctatcttctg
    63001 accatcaatg gtgagtatcc tgttttgaaa gtaacagaaa gagggataca agtattgaaa
    63061 ggacaagaat ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa
    63121 accgatacac tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc
    63181 gttcccccgt atgtcgtatt ttcagattcc acattgaaag aaatgagccg aatacgtcca
    63241 tcaagcagat tggagatgct tcagataaaa ggagttggcc aaagcaagct tgataaatat
    63301 ggcgaagcct ttttatctcg gataaaaaat gcggactcta acgttctgga taagtagaaa
    63361 gaacgaatac gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt tgctccttat
    63421 gaaaatcaaa tatgttataa ttaaggaatt agaatgggag gtaagcacag atggattctt
    63481 tgtcgaaaaa acttgcgatc caagtattaa atgatgaagc agaacggatc aaacgattaa
    63541 tccgaaatca aaacaacagt ctctgtatct cacaatgcaa agccttcgaa gaagtggtag
    63601 atacacagat gtacggcttt tcccggcaag tcggttttgc tgtccgtata ggagtaatcg
    63661 gaaagaacga gggccagctg atgttaagtg agctagaacg agaattgaat cagttatata
    63721 gtgaggttta ccaagaaatt tatgataaaa aagaagttgg caaggaggaa tagacttgcc
    63781 taagaaagtg aaaaaaaggc atctattaga ctatagcatc ttgattccct atttaatttt
    63841 atgtgtattg ggattgatca tggtatatag ttctacgtcg tatctattat tagaaaacgg
    63901 acaaaatccg tccgcatcag tcattaacca gagtattttt tgggtgctta gcttgattgc
    63961 tatcgcacta ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc
    64021 agccatcgca gttttgacga ttttgctgct gattgttgtg ttttttggta aggagatcaa
    64081 tggggcgaaa ggttggctgc aaattgcagg attctcgatc caacctgccg aatacctaaa
    64141 aatcatttct atctggtacc tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa
    64201 agattttctt gggactgtga aacgtccact tgcaatggtt atcggactta ctgcattagt
    64261 cgctattctt cccgattttg gtaatgcggc agttatcttt ttgattattt tggttctgct
    64321 tttagccagc ggagtcaatt atgtgtatac tttgattgta ggagtaggag gcttttgtct
    64381 aagtactttt acgatctggc tgatcaatat cacaaacgga aagatcttac ccggacgttt
    64441 acagtatatc tataatcgat ttgctattta ccaaaatcct ttctcagacg aattaaataa
    64501 agggcatcaa ctagtcaacg gctactatgc catgttcaac ggaggactat ttggcagggg
    64561 attaggtaac agtatccaga aaaaaggatt tttacaagaa gcacaaaccg attttattta
    64621 tgcaatcgta gtggaagaac tgggagtgat tatggggatt ttgatattag cgttgctatt
    64681 ctttatgatt gtgcgcattg tccttgtagg tatccgttca aaagatccgt tcaattcgct
    64741 tttgtgcatt gggatcggcg caatgttttt gatccaagta tttgtcaatc ttggaggaat
    64801 tactggcgtt attcctttga cagggatcac gttcccattt ttaagtcaag gaggctccag
    64861 tttgctgatg ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg
    64921 caagagctta ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag
    64981 ttgctaacag gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta
    65041 aaactgttgg aatctatgca gaagaagatg aatattccgt acaccgtttc aaagcggatg
    65101 aagcatattt agtaggacaa ggaaaaaaac cgattgatgc gtatctagat attgaaggga
    65161 tcatttctat cgcgaaagaa tgcggagctg acgctattca tccgggttac ggtcttttgt
    65221 cggaaaactt gaattttgcg caacggtgcg aagaagaagg catcattttt gttggaccca
    65281 aactgcatca tttagatatt tttggagata aaatcaaagc aaaagctgca gctatcgaag
    65341 cggggattgc ttcgattcca ggcacagatg gaccaatcgc ttctatagac gatgccttgg
    65401 agtttgcgaa acaatacggt tatccgatta tgatcaaggc agctctaggc ggcggcggac
    65461 gcggcatgcg tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa
    65521 gcgaagcaaa agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc
    65581 ctaagcatat cgaagtacag attctcgggg atacgcatgg aaacgtcatc catctttttg
    65641 aaagagattg ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct
    65701 ctatgaatga acagcaacgt caaaaaatct gccaggcagc tgtccaattg atgaaatatg
    65761 tcgggtatgt caacgcaggt acagtagaat ttcttgttga aggagatgat ttttacttta
    65821 tcgaagtcaa ccctcgcgtc caagtggaac atacgatcac agaaatgatt acagatattg
    65881 atatcgtaac aacgcaactg ttgattgctc aaggtttgga tctgcataaa gaaatcggat
    65941 tgcctcagca agaaggaatt aaattaaatg gttcagccat tcaatgccga atcactacag
    66001 aagacccgtt gaataacttt ttaccagaca cagggaaaat cgatacgtat cgttcacctg
    66061 gtggttttgg cgtacgtctg gatgtcggaa atgcttacgc gggctatgta gtgacaccgt
    66121 attttgattc attgctagta aaagtatgta cgcatggtgc tacctttgaa acagccattc
    66181 agaaaatgga acgttgcttg aaagaattta gaatccgtgg agtgaaaaca aacattccat
    66241 ttatgctcaa tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa acaaccttca
    66301 ttgatagtac agcaacatta tttgaatttc ctcgactacg agaccgcgga aacaaaacga
    66361 tgaaatatat cggtgaaatt actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac
    66421 cattttatga agaaccacgt atgcctaaag atctaatcac aagaactgat tacgtaacgg
    66481 ctaagaatgt attagatgcg aagggtgccg atgctttggt cgaatggatc aaaggacagg
    66541 aaaatttgtt gttgacggat acgacattta gagacgccca tcaaagtctg ctagcaactc
    66601 gtgttcggac aaaggatttc aagcagattg cacgtttgac tggagaaggt cttcctgaac
    66661 tcttttcaag tgagatgtgg gggggagcaa catttgatgt ggcttaccgc tttttgaatg
    66721 aagatccttg gcaacggttg agaaaaattc gttctttaat gcctaatacg ttgctgcaaa
    66781 tgcttttcag aggttcaaat gccgttgggt attctaatta tcctgacaat gtattggtgg
    66841 aatttgtcaa agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga
    66901 actggactcc acaaatggaa aaaagtatcc aagctgtacg ggatactggt aaaatcgcag
    66961 aggcagctat ttgctatact ggcgatatca acgatccaag tcgggcaaaa tacaatgttc
    67021 agtattataa agacatggca aaggaactag aacaactggg tgcgcatatc attgcaatca
    67081 aagatatggc tgggctgctt aaaccgcagg cagcttatcg tttgattagt gaattgaaag
    67141 aaacgacaga tctgccaatc catctgcata ctcatgatac cagcgggaat ggaatcatca
    67201 cttattctgc tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg agtgcaatga
    67261 gtgggaatac tagtcagcct agcatgagca gcttgtatta tgcactggtc aatggtccac
    67321 ggctgccaga aatcacgatt gaaaatgcac agaaattaaa ccattattgg gaagatgtgc
    67381 gcatgtatta caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc
    67441 atgaaatgcc aggaggacag tattcgaatc tacagcaaca ggcaaaagct gtcggattag
    67501 gtcatcgctg ggatgaaatc aaacaaatgt atcatactgt caacttgatg tttggcgata
    67561 tcgttaaagt aactccatca tctaaagtag ttggagatat ggcactattt atggtccaaa
    67621 atgatcttac agaagaagat atttatgaaa aaggcgaaac actaagtttc cctgaatctg
    67681 ttgtgacttt cttccaagga gaactagggc aacccgttgg cggatttcct aaaaaattgc
    67741 aaaagattat tcttaaaggc agacctgcat tgaatgaacg tccaggtctt ttggctgaat
    67801 cagtcgactt caacgaagta aaaaaagaat tggcagaaaa aattggctat gaaccaaaac
    67861 aagacgaagt attaagctat ttgatgtatc cgcaagtctt tttggactat caaaaagcat
    67921 ataatcaatt tggcgatgtt acattactgg acacaccaac atttttccaa ggtatacgtt
    67981 tgggtgaaac aatcaatgta caaatcgaac gcggaaaaac gcttatcatt cgtttagatg
    68041 aaatcggaga acccgatatc gaagggaatc gcgtactgtt cttcaatttg aatggacaaa
    68101 gacgtgaaat cactatcaaa gataattcga tcatcagtac ggtccaaacc cgattgaaag
    68161 ctgaaccgac aaaccgagaa caaattggag caacgatgtc tggttctgtt ttggatgtat
    68221 tagtgaaaaa aggcgataac gttaaaaaag gtgatacgtt gatgattact gaagcgatga
    68281 aaatggaaac ggccatcgaa gcacgttttg acggagaagt cgcacatgta tatgtgtctt
    68341 caggagacac tatttcgtct ggtgaccttt tgattgaagt gactgagaaa taataagcga
    68401 aaggtgggga aacatgaaaa gactgggttt gttttggctg attctgctat tagttattgt
    68461 ggcattctac atggaaccag tcttgtttcc gccaaaactt gaagtgaagg aaaactctga
    68521 tcaacaaata tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag caactggttt
    68581 ttctacttat atcggaaaac cgatcgatca gctggaaaag gattttggcc aagcctatga
    68641 tcgattaact agcggatttg gttttgaaac aagatattat gaaaataaag ctagtcatct
    68701 cacatttgaa gcgaacatcc aagacagcag agtcacaact gttaaagtac tgcaaacagg
    68761 gcctgctgat atcgcgccgt tttatttagg gatgacgatg caagatctta cgaaaatcac
    68821 tactatctat actaatttca cttttgagta ccatgataca gaagttggaa tcgaactaat
    68881 ggaagaggat atgaattatc gtccgctaat tgcttttgat aacgacactt ttgctatttt
    68941 attttttgat caaacaacaa acggcctatt ttcagttgtg tatttagata aagatagtct
    69001 tttgaaacta ttgccatatc aagtgtttgg tgaaggcctt ccacattatc aagcagataa
    69061 atcggctgat tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga ctttattaaa
    69121 tatgcttaga agagaagacg aacttcccat atacaatcaa acacttgcct ttcaaaacca
    69181 cagtcagctg ctactacatg attttttgac aaggcctgag gaaatattgt cagaggaacg
    69241 tatggctgat tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat tctcactgac
    69301 gaatgatgaa ttggagaagt taatcagtaa acaaaaatta gaaaatacta gtgggctatt
    69361 tattcatccg gcgattgacg ctactttttc gtttttatat tggtacagcg atccttacaa
    69421 ccatgaccgt ttcatggacc cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa
    69481 tatgttagtc ttattgcaag aagaaaacgg agaaagtagt gatagcagtg atcgttaacg
    69541 aagagctgtt tcaactggag gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta
    69601 aaacgatggc ttcttatctt gaacataaac agtcaatgga agaatctgca aaagtttgcc
    69661 agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg gcatatggtg
    69721 agtatgcacc tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg
    69781 atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa agtctgttgg
    69841 atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta gatgcaggta
    69901 gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat gcaattagac
    69961 aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa
    70021 caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag aatgaaatat
    70081 gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat ccgtaaatta
    70141 cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa ttttgccgaa
    70201 aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga gctaaataca
    70261 gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta aataaaaaat
    70321 aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta tactagtacc
    70381 agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg gcgggcgccg
    70441 tttaaaaagt ttagcagggg cgaacaccag accaactaca gataaagtaa aagaatccat
    70501 attcaatatg attggaccat attttgatgg agagacagta ttggatctgt ttgcaggaag
    70561 cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat gcgtagacaa
    70621 aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag aaccagagaa
    70681 atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag cggagaaaca
    70741 acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat
    70801 ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat gcgaaacgga
    70861 taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa aacagattta
    70921 tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg tgcattattc
    70981 ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg tgcggctaaa
    71041 ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag tttgtttccc
    71101 ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc
    71161 atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc agatgcgttg
    71221 atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc tcagatgaat
    71281 catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga atatagtcac
    71341 gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag cagctactta
    71401 ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag ctaaacaatg
    71461 gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg tagtagttcc
    71521 cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac taattacggt
    71581 caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg gtatcaggca
    71641 agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat tgatcagtga
    71701 agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac agtattatat
    71761 ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca agccttataa
    71821 aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt tctttggcaa
    71881 gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa gtacagaagc
    71941 atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca gttatttgca
    72001 taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag ataaaaaggc
    72061 aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag atgttcgtat
    72121 taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag aaatctatga
    72181 acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa
    72241 cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa gtgcggataa
    72301 agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga
    72361 atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa aattgaattc
    72421 ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc tagagaaaat
    72481 gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca acccctcttt
    72541 ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg taattagatg
    72601 ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa aggagtaatg
    72661 aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa
    72721 atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat cttttgtatc
    72781 gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga gactgctgca
    72841 aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga aggaaacgat
    72901 gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag
    72961 cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt
    73021 ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat agaagatcag
    73081 caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga tcagttggca
    73141 tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa tatcaataca
    73201 gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc gcaagaaatc
    73261 attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca ggagatttct
    73321 ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa atagaatgat
    73381 tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa taatccaatt
    73441 ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct tgattgttcg
    73501 caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa tatctgggat
    73561 tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag aacgctttgc
    73621 caaagatgta cttagctata cagcagataa agtatttatt ctcattggga cgaatgatct
    73681 agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt
    73741 cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc agcagtcgat
    73801 gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc tgttctcaca
    73861 gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt cttgaacaaa
    73921 tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc ggcagtgaag
    73981 ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt tgtttaagca
    74041 atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct
    74101 tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa tgaacgcatt
    74161 ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag tacgtgtaca
    74221 cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg cggctataat
    74281 ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt ggatggacac
    74341 tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa acttggtatc
    74401 ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg tacaaaggct
    74461 ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa tgatccttat
    74521 gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt agactcacag
    74581 catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt tagaaaggca
    74641 tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt tgttgtatta
    74701 cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt tttcgctaaa
    74761 agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat ccatttctta
    74821 taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt
    74881 tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt tatcagagaa
    74941 taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga aaacatggct
    75001 taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct ctccagcaaa
    75061 aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata gaatttctgg
    75121 acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc gaaactggct
    75181 tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc aaaaaacaag
    75241 tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag aaccagaaga
    75301 aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac acatccaatt
    75361 ttatttaggg tggctgtatt atttatttcg acgtatcgga tggccactac acgtcagtat
    75421 tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt ctatttctgt
    75481 tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga ataagcatta
    75541 ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg atcctcgatt
    75601 tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta tcctgatttc
    75661 ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat
    75721 gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt ttacattact
    75781 gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca cactcttcaa
    75841 tcgctggctg ccggaaactt tacttttgct gatagaagag ggattaatct tatttgagcg
    75901 aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc ttgtgctatc
    75961 tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac aacctgtgaa
    76021 acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat ttttacgctt
    76081 ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc
    76141 ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg aaagagaaaa
    76201 gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat atctaaaagg
    76261 tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg atcatgtggg
    76321 agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg gttggggcgc
    76381 tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg gtactgagat
    76441 cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg tattgactcc
    76501 ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg aatataacaa
    76561 taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat
    76621 ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc gtacgtcaag
    76681 taacacagat tttctttccc atattgaagc aaaacatggg attatatcct gcggcgtaaa
    76741 taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa atcagataaa
    76801 aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag tttttcatcc
    76861 tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg gtaggataat
    76921 ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa
    76981 aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat caagttcgga
    77041 cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt ttaagttttg
    77101 acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg ccgtttttcg
    77161 gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa aaagtaaaca
    77221 atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca ttagaatcga
    77281 ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa gtaacgaaac
    77341 aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc
    77401 aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt gcaattgatt
    77461 tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta cagaaattgg
    77521 tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa ttagttccta
    77581 aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg aagacggaac
    77641 aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc aaaattaatg
    77701 cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta aagattggtt
    77761 atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta aaacttgcca
    77821 tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat caattagttg
    77881 agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt caactattcg
    77941 ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg caaagcatgc
    78001 gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc gaagccgatt
    78061 cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt tgtttttgtg
    78121 gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat ataacgcatc
    78181 tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag tacgcatagc
    78241 gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac gtttgattgc
    78301 agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg cgtgttaccg
    78361 cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca
    78421 agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg gatagtacaa
    78481 gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc aaactagatt
    78541 tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt tattatataa
    78601 caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg ttgttctttg
    78661 tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca gtaaaggcta
    78721 ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca ttttttttat
    78781 gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt tatttagggg
    78841 caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga tcacacggtc
    78901 ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc
    78961 aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa
    79021 aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca ttctttcttt
    79081 tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat
    79141 cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat tgattaaaaa
    79201 aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc aaaaagcagt
    79261 atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca gaatcaaaag
    79321 taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt cgaacattgt
    79381 atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga atctcctaaa
    79441 atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag taagtgatac
    79501 cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc
    79561 cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca cccttggaag
    79621 ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg gatttaaagg
    79681 cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga atagccgctg
    79741 tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa aaggaacggg
    79801 aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga ctttgatttt
    79861 tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc atgaaatata
    79921 ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa agtaggaatt
    79981 catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa tcataaaaaa
    80041 acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag acttacattt
    80101 ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat tcagaataac
    80161 caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa attgcaggaa
    80221 gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt gtttgatgat
    80281 ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt cgcttttcat
    80341 taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag cgacacctaa
    80401 agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg catttacttg
    80461 gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac agcttgcata
    80521 gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat ggtcgccggt
    80581 tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt gggtatttgc
    80641 ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag aaagataaat
    80701 cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt tgctgattat
    80761 catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta aataggtagt
    80821 ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt caggtattgt
    80881 gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt gttccatagc
    80941 aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag ttacaactgg
    81001 ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt cagaaagctt
    81061 ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg aaagagcagc
    81121 tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa ctaataatac
    81181 cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg ggagaaggat
    81241 cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc gttcgataaa
    81301 tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa tcttagcaaa
    81361 gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt gattcagcgt
    81421 tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac cgcttaatgc
    81481 agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa cagctgctcc
    81541 tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt cttgcatttg
    81601 tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag cagtaatttc
    81661 tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa agatgaatgt
    81721 caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag ctaaagccat
    81781 caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt ccgatgtttg
    81841 tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg taagagagaa
    81901 tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg ctgcaaaagc
    81961 ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata gcataataaa
    82021 aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat ttggtgatta
    82081 agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa aaaaccagaa
    82141 catttctcaa ataaga
//
LOCUS       DAZBTH010000003        73663 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000003 DAZBTH010000000
VERSION     DAZBTH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.003"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHGNW_000165"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHGNW_000165"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN6924519.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHGNW_000166"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHGNW_000166"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN6924520.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHGNW_000167"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHGNW_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN6924521.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHGNW_000168"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHGNW_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN6924522.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHGNW_000169"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHGNW_000169"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN6924523.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHGNW_000170"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHGNW_000170"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN6924524.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHGNW_000171"
     CDS             complement(8053..8523)
                     /locus_tag="ACHGNW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN6924525.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHGNW_000172"
     CDS             complement(8569..8994)
                     /locus_tag="ACHGNW_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN6924526.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHGNW_000173"
     CDS             complement(9061..9330)
                     /locus_tag="ACHGNW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN6924527.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHGNW_000174"
     CDS             complement(9341..10936)
                     /locus_tag="ACHGNW_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6924528.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHGNW_000175"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHGNW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN6924529.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHGNW_000176"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHGNW_000176"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN6924530.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHGNW_000177"
     CDS             15402..16376
                     /locus_tag="ACHGNW_000177"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6924531.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHGNW_000178"
     CDS             16546..17946
                     /locus_tag="ACHGNW_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN6924532.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHGNW_000179"
     CDS             complement(18024..18746)
                     /locus_tag="ACHGNW_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6924533.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHGNW_000180"
     CDS             complement(18825..19331)
                     /locus_tag="ACHGNW_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6924534.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHGNW_000181"
     CDS             19454..21253
                     /locus_tag="ACHGNW_000181"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN6924535.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHGNW_000182"
     CDS             complement(21321..22895)
                     /locus_tag="ACHGNW_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN6924536.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHGNW_000183"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHGNW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN6924537.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHGNW_000184"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHGNW_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN6924538.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHGNW_000185"
     CDS             complement(24672..25310)
                     /locus_tag="ACHGNW_000185"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN6924539.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHGNW_000186"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHGNW_000186"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN6924540.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHGNW_000187"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHGNW_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN6924541.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHGNW_000188"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHGNW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN6924542.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHGNW_000189"
     CDS             complement(29104..29844)
                     /locus_tag="ACHGNW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN6924543.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHGNW_000190"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHGNW_000190"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN6924544.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHGNW_000191"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHGNW_000191"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN6924545.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHGNW_000192"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHGNW_000192"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6924546.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHGNW_000193"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHGNW_000193"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN6924547.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHGNW_000194"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHGNW_000194"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6924548.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHGNW_000195"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHGNW_000195"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6924549.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACHGNW_000196"
     CDS             complement(36724..37008)
                     /locus_tag="ACHGNW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924550.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACHGNW_000197"
     CDS             complement(37026..37916)
                     /locus_tag="ACHGNW_000197"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN6924551.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACHGNW_000198"
     CDS             complement(37936..38154)
                     /locus_tag="ACHGNW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN6924552.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACHGNW_000199"
     CDS             complement(38124..39824)
                     /locus_tag="ACHGNW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN6924553.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACHGNW_000200"
     CDS             40091..40864
                     /locus_tag="ACHGNW_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924554.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHGNW_000201"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHGNW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN6924555.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACHGNW_000202"
     CDS             complement(41688..42659)
                     /locus_tag="ACHGNW_000202"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6924556.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHGNW_000203"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHGNW_000203"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN6924557.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHGNW_000204"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHGNW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN6924558.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ACHGNW_000205"
     CDS             complement(45614..46438)
                     /locus_tag="ACHGNW_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN6924559.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ACHGNW_000206"
     CDS             complement(46411..47091)
                     /locus_tag="ACHGNW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6924560.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ACHGNW_000207"
     CDS             complement(47114..48052)
                     /locus_tag="ACHGNW_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN6924561.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACHGNW_000208"
     CDS             complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACHGNW_000208"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN6924562.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACHGNW_000209"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACHGNW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6924563.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ACHGNW_000210"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ACHGNW_000210"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN6924564.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACHGNW_000211"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACHGNW_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN6924565.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACHGNW_000212"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACHGNW_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN6924566.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ACHGNW_000213"
     CDS             complement(56026..57267)
                     /locus_tag="ACHGNW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN6924567.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACHGNW_000214"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACHGNW_000214"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN6924568.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ACHGNW_000215"
     CDS             complement(58294..59217)
                     /locus_tag="ACHGNW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN6924569.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ACHGNW_000216"
     CDS             complement(59956..61242)
                     /locus_tag="ACHGNW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6924570.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ACHGNW_000217"
     CDS             complement(61235..62503)
                     /locus_tag="ACHGNW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6924571.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="ACHGNW_000218"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="ACHGNW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN6924572.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="ACHGNW_000219"
     CDS             complement(63276..63722)
                     /locus_tag="ACHGNW_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924573.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="ACHGNW_000220"
     CDS             complement(63815..64060)
                     /locus_tag="ACHGNW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN6924574.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="ACHGNW_000221"
     CDS             complement(64175..64753)
                     /locus_tag="ACHGNW_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6924575.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="ACHGNW_000222"
     CDS             complement(64830..65360)
                     /locus_tag="ACHGNW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN6924576.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACHGNW_000223"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACHGNW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN6924577.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="ACHGNW_000224"
     CDS             complement(66217..66612)
                     /locus_tag="ACHGNW_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN6924578.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACHGNW_000225"
     CDS             complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACHGNW_000225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN6924579.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACHGNW_000226"
     CDS             complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACHGNW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN6924580.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68640..68879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACHGNW_000227"
     CDS             complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACHGNW_000227"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN6924581.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="ACHGNW_000228"
     CDS             complement(70506..71663)
                     /locus_tag="ACHGNW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN6924582.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACHGNW_000229"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACHGNW_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN6924583.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="ACHGNW_000230"
     CDS             complement(73130..73657)
                     /locus_tag="ACHGNW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN6924584.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        20135 a        16072 c        13210 g        24246 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttt
//
LOCUS       DAZBTH010000004        68348 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000004 DAZBTH010000000
VERSION     DAZBTH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.004"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..742
                     /locus_tag="ACHGNW_000231"
     CDS             <1..742
                     /locus_tag="ACHGNW_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFN6924585.1"
                     /translation="KSLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDETDDFTYYK
                     GYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPE
                     NYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITV
                     PRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNT
                     NIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            803..1303
                     /locus_tag="ACHGNW_000232"
     CDS             803..1303
                     /locus_tag="ACHGNW_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN6924586.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            1359..1997
                     /locus_tag="ACHGNW_000233"
     CDS             1359..1997
                     /locus_tag="ACHGNW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFN6924587.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2069..2464
                     /locus_tag="ACHGNW_000234"
     CDS             2069..2464
                     /locus_tag="ACHGNW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6924588.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2448..4901
                     /locus_tag="ACHGNW_000235"
     CDS             2448..4901
                     /locus_tag="ACHGNW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6924589.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4898..6925
                     /locus_tag="ACHGNW_000236"
     CDS             4898..6925
                     /locus_tag="ACHGNW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6924590.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6922..7944
                     /locus_tag="ACHGNW_000237"
     CDS             6922..7944
                     /locus_tag="ACHGNW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFN6924591.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7961..8890
                     /locus_tag="ACHGNW_000238"
     CDS             7961..8890
                     /locus_tag="ACHGNW_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6924592.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9204..9251
                     /locus_tag="ACHGNW_000239"
                     /pseudo
     CDS             9204..9251
                     /locus_tag="ACHGNW_000239"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9267..10067
                     /locus_tag="ACHGNW_000240"
                     /pseudo
     CDS             9267..10067
                     /locus_tag="ACHGNW_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            10305..10541
                     /locus_tag="ACHGNW_000241"
     CDS             10305..10541
                     /locus_tag="ACHGNW_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924593.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10548..12500
                     /locus_tag="ACHGNW_000242"
     CDS             10548..12500
                     /locus_tag="ACHGNW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6924594.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12572..13069)
                     /gene="dfrG"
                     /locus_tag="ACHGNW_000243"
     CDS             complement(12572..13069)
                     /gene="dfrG"
                     /locus_tag="ACHGNW_000243"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6924595.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            13344..14471
                     /locus_tag="ACHGNW_000244"
                     /pseudo
     CDS             13344..14471
                     /locus_tag="ACHGNW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            14569..14757
                     /locus_tag="ACHGNW_000245"
     CDS             14569..14757
                     /locus_tag="ACHGNW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFN6924596.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14815..15168)
                     /locus_tag="ACHGNW_000246"
     CDS             complement(14815..15168)
                     /locus_tag="ACHGNW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6924597.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15699..16121
                     /locus_tag="ACHGNW_000247"
     CDS             15699..16121
                     /locus_tag="ACHGNW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6924598.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            16118..16348
                     /locus_tag="ACHGNW_000248"
     CDS             16118..16348
                     /locus_tag="ACHGNW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6924599.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16846..17046
                     /locus_tag="ACHGNW_000249"
     CDS             16846..17046
                     /locus_tag="ACHGNW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFN6924600.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            17073..18266
                     /locus_tag="ACHGNW_000250"
     CDS             17073..18266
                     /locus_tag="ACHGNW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6924601.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(18334..19899)
                     /gene="guaA"
                     /locus_tag="ACHGNW_000251"
     CDS             complement(18334..19899)
                     /gene="guaA"
                     /locus_tag="ACHGNW_000251"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6924602.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(19958..20881)
                     /gene="coaA"
                     /locus_tag="ACHGNW_000252"
     CDS             complement(19958..20881)
                     /gene="coaA"
                     /locus_tag="ACHGNW_000252"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN6924603.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            21077..21682
                     /locus_tag="ACHGNW_000253"
     CDS             21077..21682
                     /locus_tag="ACHGNW_000253"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6924604.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            21990..23006
                     /locus_tag="ACHGNW_000254"
     CDS             21990..23006
                     /locus_tag="ACHGNW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6924605.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            23147..24448
                     /locus_tag="ACHGNW_000255"
     CDS             23147..24448
                     /locus_tag="ACHGNW_000255"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN6924606.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24494..25156
                     /gene="deoC"
                     /locus_tag="ACHGNW_000256"
     CDS             24494..25156
                     /gene="deoC"
                     /locus_tag="ACHGNW_000256"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN6924607.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            25177..25575
                     /locus_tag="ACHGNW_000257"
     CDS             25177..25575
                     /locus_tag="ACHGNW_000257"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN6924608.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25641..26723
                     /locus_tag="ACHGNW_000258"
     CDS             25641..26723
                     /locus_tag="ACHGNW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6924609.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            26935..28497
                     /locus_tag="ACHGNW_000259"
     CDS             26935..28497
                     /locus_tag="ACHGNW_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924610.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28490..29623
                     /locus_tag="ACHGNW_000260"
     CDS             28490..29623
                     /locus_tag="ACHGNW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924611.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29623..30579
                     /locus_tag="ACHGNW_000261"
     CDS             29623..30579
                     /locus_tag="ACHGNW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924612.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30678..31844
                     /gene="deoB"
                     /locus_tag="ACHGNW_000262"
     CDS             30678..31844
                     /gene="deoB"
                     /locus_tag="ACHGNW_000262"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN6924613.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            31868..32686
                     /locus_tag="ACHGNW_000263"
     CDS             31868..32686
                     /locus_tag="ACHGNW_000263"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6924614.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32703..33410
                     /gene="deoD"
                     /locus_tag="ACHGNW_000264"
     CDS             32703..33410
                     /gene="deoD"
                     /locus_tag="ACHGNW_000264"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6924615.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(33708..34394)
                     /gene="gpmA"
                     /locus_tag="ACHGNW_000265"
     CDS             complement(33708..34394)
                     /gene="gpmA"
                     /locus_tag="ACHGNW_000265"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6924616.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(34566..35147)
                     /locus_tag="ACHGNW_000266"
     CDS             complement(34566..35147)
                     /locus_tag="ACHGNW_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6924617.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(35280..35963)
                     /gene="rpiA"
                     /locus_tag="ACHGNW_000267"
     CDS             complement(35280..35963)
                     /gene="rpiA"
                     /locus_tag="ACHGNW_000267"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN6924618.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            36282..36695
                     /gene="rpsL"
                     /locus_tag="ACHGNW_000268"
     CDS             36282..36695
                     /gene="rpsL"
                     /locus_tag="ACHGNW_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN6924619.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            36779..37249
                     /gene="rpsG"
                     /locus_tag="ACHGNW_000269"
     CDS             36779..37249
                     /gene="rpsG"
                     /locus_tag="ACHGNW_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN6924620.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            37343..39430
                     /gene="fusA"
                     /locus_tag="ACHGNW_000270"
     CDS             37343..39430
                     /gene="fusA"
                     /locus_tag="ACHGNW_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN6924621.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            39602..40789
                     /gene="tuf"
                     /locus_tag="ACHGNW_000271"
     CDS             39602..40789
                     /gene="tuf"
                     /locus_tag="ACHGNW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6924622.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            41196..41504
                     /gene="rpsJ"
                     /locus_tag="ACHGNW_000272"
     CDS             41196..41504
                     /gene="rpsJ"
                     /locus_tag="ACHGNW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN6924623.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            41535..42164
                     /gene="rplC"
                     /locus_tag="ACHGNW_000273"
     CDS             41535..42164
                     /gene="rplC"
                     /locus_tag="ACHGNW_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN6924624.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            42192..42815
                     /gene="rplD"
                     /locus_tag="ACHGNW_000274"
     CDS             42192..42815
                     /gene="rplD"
                     /locus_tag="ACHGNW_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN6924625.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            42815..43105
                     /gene="rplW"
                     /locus_tag="ACHGNW_000275"
     CDS             42815..43105
                     /gene="rplW"
                     /locus_tag="ACHGNW_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN6924626.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            43144..43977
                     /gene="rplB"
                     /locus_tag="ACHGNW_000276"
     CDS             43144..43977
                     /gene="rplB"
                     /locus_tag="ACHGNW_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN6924627.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            44020..44298
                     /gene="rpsS"
                     /locus_tag="ACHGNW_000277"
     CDS             44020..44298
                     /gene="rpsS"
                     /locus_tag="ACHGNW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN6924628.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            44321..44668
                     /gene="rplV"
                     /locus_tag="ACHGNW_000278"
     CDS             44321..44668
                     /gene="rplV"
                     /locus_tag="ACHGNW_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN6924629.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            44682..45338
                     /gene="rpsC"
                     /locus_tag="ACHGNW_000279"
     CDS             44682..45338
                     /gene="rpsC"
                     /locus_tag="ACHGNW_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN6924630.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            45341..45775
                     /gene="rplP"
                     /locus_tag="ACHGNW_000280"
     CDS             45341..45775
                     /gene="rplP"
                     /locus_tag="ACHGNW_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN6924631.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            45765..45953
                     /gene="rpmC"
                     /locus_tag="ACHGNW_000281"
     CDS             45765..45953
                     /gene="rpmC"
                     /locus_tag="ACHGNW_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN6924632.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            45978..46244
                     /gene="rpsQ"
                     /locus_tag="ACHGNW_000282"
     CDS             45978..46244
                     /gene="rpsQ"
                     /locus_tag="ACHGNW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN6924633.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            46302..46670
                     /gene="rplN"
                     /locus_tag="ACHGNW_000283"
     CDS             46302..46670
                     /gene="rplN"
                     /locus_tag="ACHGNW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN6924634.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            46707..47015
                     /gene="rplX"
                     /locus_tag="ACHGNW_000284"
     CDS             46707..47015
                     /gene="rplX"
                     /locus_tag="ACHGNW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN6924635.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            47043..47582
                     /gene="rplE"
                     /locus_tag="ACHGNW_000285"
     CDS             47043..47582
                     /gene="rplE"
                     /locus_tag="ACHGNW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN6924636.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            47601..47786
                     /locus_tag="ACHGNW_000286"
     CDS             47601..47786
                     /locus_tag="ACHGNW_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN6924637.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            47822..48220
                     /gene="rpsH"
                     /locus_tag="ACHGNW_000287"
     CDS             47822..48220
                     /gene="rpsH"
                     /locus_tag="ACHGNW_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN6924638.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            48252..48788
                     /gene="rplF"
                     /locus_tag="ACHGNW_000288"
     CDS             48252..48788
                     /gene="rplF"
                     /locus_tag="ACHGNW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN6924639.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            48949..49305
                     /gene="rplR"
                     /locus_tag="ACHGNW_000289"
     CDS             48949..49305
                     /gene="rplR"
                     /locus_tag="ACHGNW_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN6924640.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            49326..49826
                     /gene="rpsE"
                     /locus_tag="ACHGNW_000290"
     CDS             49326..49826
                     /gene="rpsE"
                     /locus_tag="ACHGNW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN6924641.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            49841..50020
                     /gene="rpmD"
                     /locus_tag="ACHGNW_000291"
     CDS             49841..50020
                     /gene="rpmD"
                     /locus_tag="ACHGNW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN6924642.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            50064..50504
                     /gene="rplO"
                     /locus_tag="ACHGNW_000292"
     CDS             50064..50504
                     /gene="rplO"
                     /locus_tag="ACHGNW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN6924643.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            50504..51799
                     /gene="secY"
                     /locus_tag="ACHGNW_000293"
     CDS             50504..51799
                     /gene="secY"
                     /locus_tag="ACHGNW_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN6924644.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            51861..52508
                     /locus_tag="ACHGNW_000294"
     CDS             51861..52508
                     /locus_tag="ACHGNW_000294"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN6924645.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            52699..52917
                     /gene="infA"
                     /locus_tag="ACHGNW_000295"
     CDS             52699..52917
                     /gene="infA"
                     /locus_tag="ACHGNW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN6924646.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            52950..53066
                     /gene="rpmJ"
                     /locus_tag="ACHGNW_000296"
     CDS             52950..53066
                     /gene="rpmJ"
                     /locus_tag="ACHGNW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN6924647.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            53084..53449
                     /gene="rpsM"
                     /locus_tag="ACHGNW_000297"
     CDS             53084..53449
                     /gene="rpsM"
                     /locus_tag="ACHGNW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN6924648.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            53477..53866
                     /gene="rpsK"
                     /locus_tag="ACHGNW_000298"
     CDS             53477..53866
                     /gene="rpsK"
                     /locus_tag="ACHGNW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN6924649.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            53946..54884
                     /locus_tag="ACHGNW_000299"
     CDS             53946..54884
                     /locus_tag="ACHGNW_000299"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN6924650.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            54912..55292
                     /gene="rplQ"
                     /locus_tag="ACHGNW_000300"
     CDS             54912..55292
                     /gene="rplQ"
                     /locus_tag="ACHGNW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN6924651.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            55564..56403
                     /locus_tag="ACHGNW_000301"
     CDS             55564..56403
                     /locus_tag="ACHGNW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6924652.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            56379..57248
                     /locus_tag="ACHGNW_000302"
     CDS             56379..57248
                     /locus_tag="ACHGNW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6924653.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            57238..58035
                     /locus_tag="ACHGNW_000303"
     CDS             57238..58035
                     /locus_tag="ACHGNW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6924654.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            58238..58984
                     /gene="truA"
                     /locus_tag="ACHGNW_000304"
     CDS             58238..58984
                     /gene="truA"
                     /locus_tag="ACHGNW_000304"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6924655.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(59317..61986)
                     /locus_tag="ACHGNW_000305"
     CDS             complement(59317..61986)
                     /locus_tag="ACHGNW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN6924656.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(62224..62412)
                     /gene="rpmB"
                     /locus_tag="ACHGNW_000306"
     CDS             complement(62224..62412)
                     /gene="rpmB"
                     /locus_tag="ACHGNW_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN6924657.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            62682..63044
                     /locus_tag="ACHGNW_000307"
     CDS             62682..63044
                     /locus_tag="ACHGNW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6924658.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            63096..64778
                     /locus_tag="ACHGNW_000308"
     CDS             63096..64778
                     /locus_tag="ACHGNW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN6924659.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            64895..66931
                     /gene="recG"
                     /locus_tag="ACHGNW_000309"
     CDS             64895..66931
                     /gene="recG"
                     /locus_tag="ACHGNW_000309"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN6924660.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            66980..67981
                     /gene="plsX"
                     /locus_tag="ACHGNW_000310"
     CDS             66980..67981
                     /gene="plsX"
                     /locus_tag="ACHGNW_000310"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN6924661.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            68103..68345
                     /gene="acpP"
                     /locus_tag="ACHGNW_000311"
     CDS             68103..68345
                     /gene="acpP"
                     /locus_tag="ACHGNW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6924662.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        22427 a        12216 c        14392 g        19313 t
ORIGIN      
        1 taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg
       61 taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt
      121 tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag
      181 agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc
      241 aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact
      301 tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca
      361 agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag
      421 agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt
      481 tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga
      541 aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat
      601 gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat
      661 tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta cagattatat
      721 tagcggatac cttgataagt agcgtcatta actgattgct acttttttta tgactatttt
      781 tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta
      841 taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc
      901 agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc
      961 ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca
     1021 agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag
     1081 tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc
     1141 gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc
     1201 caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa
     1261 ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt
     1321 ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc
     1381 ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc
     1441 gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc
     1501 cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag
     1561 ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga gaatattttg
     1621 gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc
     1681 gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt
     1741 tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa
     1801 gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa
     1861 cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca
     1921 gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa
     1981 aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt
     2041 tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg
     2101 gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt
     2161 tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc
     2221 accactttct ctgattgacg gagcactact caaatactta gggattccca ttgttctcac
     2281 tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat
     2341 cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa
     2401 agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga
     2461 ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg
     2521 aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata
     2581 atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa
     2641 ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa
     2701 aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg
     2761 acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag
     2821 tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg
     2881 tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt
     2941 ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg
     3001 agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg
     3061 atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat
     3121 acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg
     3181 aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat
     3241 tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg
     3301 ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata
     3361 aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta
     3421 ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca
     3481 aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc
     3541 tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac
     3601 gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata
     3661 tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta
     3721 cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa
     3781 atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat
     3841 tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg
     3901 tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg
     3961 gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga
     4021 gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt
     4081 cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg
     4141 agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt
     4201 tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg
     4261 accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg
     4321 tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct
     4381 tacccgattc caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa
     4441 tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta
     4501 aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat
     4561 ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa
     4621 acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc
     4681 gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag
     4741 atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca
     4801 tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct
     4861 ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt
     4921 tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac
     4981 gctgggcaat gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga
     5041 atactccaag tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg
     5101 ctggcttcca tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc
     5161 cattactaat tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt
     5221 gaaagaagcc tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat
     5281 gcagacgatt gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt
     5341 cctccttctt tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg
     5401 agaaacgacg aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc
     5461 ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt
     5521 cagtaccgcc agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg
     5581 gaaagacagt gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg
     5641 gctattgctt caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag
     5701 tttattgtct accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga
     5761 agaaattgaa gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg
     5821 aacagtcttc tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac
     5881 aggaatcatg attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag
     5941 tttcctatta agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt
     6001 atttaatgtg attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat
     6061 ttccactatg ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca
     6121 aattgtgacc tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt
     6181 acaaagcaac gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat
     6241 gaatcgacaa accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg
     6301 gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac
     6361 tcgtcaaaat gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca
     6421 acaaggcggt atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa
     6481 tcgtctagtc aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt
     6541 caaatatgcc cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca
     6601 atctatggca gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa
     6661 acgaaagaac attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa
     6721 agaaaaccca tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac
     6781 cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa
     6841 agagcaaaaa caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac
     6901 ggataagcga ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc
     6961 tctctcttct tttttctctt attattccta gtcattttct ttgcagatga tacggacagt
     7021 ggcgaaaaca acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg
     7081 ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt
     7141 tcttatatct tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa
     7201 agttccgaat ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa
     7261 ggggttaaat atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat
     7321 tctgttatcc aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg
     7381 aaaaaatata cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa
     7441 gcagactatc ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga
     7501 aatcagtttt atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt
     7561 gatgatgaaa ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat
     7621 gtctttggag gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg
     7681 tatgacaaag ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa
     7741 gaaatctcta tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat
     7801 gcaggcacat atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca
     7861 ggcgacccaa tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg
     7921 gctagacgtg ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg
     7981 aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc
     8041 gatgattaaa gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg
     8101 tagtgtcgga tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga
     8161 aacagaaatc attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc
     8221 ctttgcgaaa gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga
     8281 aaaattgacc cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa
     8341 ggatattcct actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa
     8401 tgagaacacc tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa
     8461 ggaaactata tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt
     8521 tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca
     8581 gctagaaagt gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga
     8641 aacgttcttc cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa
     8701 tcatgtgtta cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt
     8761 taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac
     8821 aaaggcaacc caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat
     8881 tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg
     8941 ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat
     9001 ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc
     9061 gggaacaaat aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag
     9121 aataccttta tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta
     9181 tggttatgca taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt
     9241 tgggtttttg aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg
     9301 ttgatgcagg aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag
     9361 aattaggaag cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga
     9421 gaggaattac aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca
     9481 tcatagacac gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt
     9541 tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat
     9601 tatttcatgc acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc
     9661 aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa
     9721 ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat
     9781 ctgaacaatg ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg
     9841 gtaaatcatt agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt
     9901 ctctgttccc tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag
     9961 aagttattac taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa
    10021 atgttttcaa aattgaatat acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt
    10081 tttggagtac aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca actgaatttg
    10141 atactcgttt tttattgtta aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt
    10201 taaaatgtat ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa attggtgtgt
    10261 taaaatggtt acaatttact aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc
    10321 caaaaaataa gaacgctaag tctgtggatt gggaaatatc agagcaagta agggtgatag
    10381 ttaagcaata cgctgaatat gcagagcgta ctgaaagcga agcagtggat gaattcttat
    10441 tgaacatttt agacgataag aagtttattg aatggattgc caacaagcga agtaataagc
    10501 ggattgttga aaaaatgggt atcaaagata gagtgggttg atagacaatg accaagctta
    10561 aaagattagc cacgaaggaa gatgaaattg tagatgttaa aattccgatt tcaaacgaag
    10621 aattaaaaga tagggctaaa caatatgatt tattaactcc aaaacgattt gcaacaaggt
    10681 ataacaaaat gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa tatcccattc
    10741 aatataatta ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag cacaaattca
    10801 aagatgtaaa agggaaacca agtcgatata aaatgactgg tagttccaaa gataaaggtc
    10861 ataaaggtat gtactgcaat gacaatccta ttggcagagg tgtatcgcaa aactgtacag
    10921 taaccccttt atctaattgg tctgttgttg aagaaattaa acgccttata gaaatcaata
    10981 gcatccaaga tgttgagcca gattatcaat ttcataaaga aggctgctcg gaagaagaat
    11041 caaccccatt caatgagccg aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc
    11101 aacgctatca atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa
    11161 ccactactta tcaccaacaa aagaacacca ttcttccaat gttcgctaag atggttgttg
    11221 gaagggtttc agtcagtcgt acttgtgata ttttaggaat tggagttgga acctactatc
    11281 ataagctgga gtggctgtat aggcgttgtt tggagttttt agaacgatat gagacacaac
    11341 cccttcaaac aaagaaattt aacgagatgt ggcttaatac ggacaagatg cactactacc
    11401 taaataacgt ccgtaagaaa ggacaaggtt caaagaaata tacggggttt gaagacctta
    11461 atatgcaaac gtatatagtt gtatctgctg aggtcttatc aagatatgtg ttccgttctg
    11521 atgttgccta tgattggaat atttcaatgg atgaattaaa tgaagataca agaaaattca
    11581 aagaagacca tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc
    11641 cacaagaacc atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga
    11701 aaattactaa taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa
    11761 ctgctcacta ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg
    11821 atgatgactt ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga
    11881 gcgatgcaca tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa
    11941 aagaattcgg acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa
    12001 caaagtttct acggactttg gcatctcatt atctaactga attactaaca agtcatcaat
    12061 tccacgaaga ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta
    12121 tcaaacatcc attagccact aaggataaag gattttattc agttgattgt agaacggatt
    12181 tatctgctct ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga
    12241 ccaatagctt tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa
    12301 cagctcgtgg tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat
    12361 ttgcgattac aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata
    12421 aaaaggtttt gacacctgct caaagattag ggattacaga taaacaattt gattggaaag
    12481 atataattta tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat
    12541 ctttactgtt cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata
    12601 catgaaaata gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct
    12661 cattccattc ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta
    12721 tttttgtgat gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc
    12781 cgaaaataaa aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa
    12841 caatttcaca accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt
    12901 caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct
    12961 ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt
    13021 tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag
    13081 cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat
    13141 cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa
    13201 atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta
    13261 atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt
    13321 gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt
    13381 tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt
    13441 caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc
    13501 aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa
    13561 aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac
    13621 agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat
    13681 attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
    13741 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta
    13801 cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc
    13861 cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg
    13921 gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag
    13981 ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt
    14041 gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc
    14101 ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag
    14161 agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg
    14221 atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta
    14281 ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta acttttttta
    14341 caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac
    14401 ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata
    14461 aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa
    14521 atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac
    14581 aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact
    14641 atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt aattaatgtt
    14701 caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag ccgataattt
    14761 gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta ctgactagcc
    14821 ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg atagttcttt
    14881 gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt taatcaaatt
    14941 atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa gatcatacag
    15001 cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac gtgggtcaat
    15061 ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag cctctttaat
    15121 cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac taaatcacct
    15181 ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat gttatatagt
    15241 ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa tttcaaatag
    15301 aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata ctcctagaaa
    15361 gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat aatctgtcat
    15421 ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg gatagatatg
    15481 accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat gaacagagaa
    15541 aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga tttctcttca
    15601 tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac
    15661 atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct tcttttcaag
    15721 aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat gaacgaaaag
    15781 attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct gatatagata
    15841 actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc caactattta
    15901 aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc
    15961 tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg agtgatgtgg
    16021 agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga ataagtggac
    16081 tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt atccaagggt
    16141 tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta atcagattgt
    16201 taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag acgaatatgg
    16261 aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga aactcatctc
    16321 taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt
    16381 atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt gtgttcagta
    16441 ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc aatcgaatac
    16501 gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat tagtgagcga
    16561 ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact ttcttatcgt
    16621 gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag gcaatgggta
    16681 cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat
    16741 tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata taattatagg
    16801 tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat aacggtcaca
    16861 aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat cataaaaaaa
    16921 gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa gctagataga
    16981 gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga aaacaggtct
    17041 gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa
    17101 agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc
    17161 aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat tcgaaactgc
    17221 tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt aaacattata
    17281 tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca tatgaaaatc
    17341 gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt tccgatattg
    17401 agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag tcttcttatg
    17461 taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc cttgggataa
    17521 ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct aaaattgaat
    17581 tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa gattactatc
    17641 aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga attggtgaag
    17701 ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc atcaacaaaa
    17761 cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca aaggcgagcg
    17821 ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg aaggaagtac
    17881 aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct acccaaaaac
    17941 atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat cgaatcagaa
    18001 tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa aatccactta
    18061 tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg tatggacatc
    18121 tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt tcctttaaag
    18181 aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac tacttgcgaa
    18241 acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga gccagaaacc
    18301 cttggtataa gaggatttct agctctccta caactattcc cactccactg ttgctggtgg
    18361 tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac
    18421 agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat
    18481 tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc
    18541 gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc
    18601 agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc
    18661 agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac
    18721 gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt
    18781 gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg
    18841 agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc
    18901 ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac
    18961 atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa
    19021 cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga attttccgcc
    19081 tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat
    19141 agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac
    19201 gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac
    19261 ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc
    19321 aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat
    19381 accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc
    19441 gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt
    19501 ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc
    19561 tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa
    19621 ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat
    19681 cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg
    19741 attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc
    19801 aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat
    19861 aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt
    19921 aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg
    19981 aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca
    20041 agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa
    20101 aggcttcttc acgatctccg atagcaaaac gataataata attctctgga ttttggaaag
    20161 ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg
    20221 catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta
    20281 attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact
    20341 tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac
    20401 cagattttac acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc
    20461 ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag
    20521 tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc
    20581 tggctgttgt actttttcca acggctacac ttccggcgat cccgataata aaaggcggta
    20641 cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca
    20701 tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa
    20761 tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat
    20821 tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca
    20881 tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc
    20941 ccaccactta agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga
    21001 gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat
    21061 gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga
    21121 ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg
    21181 tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg
    21241 ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg
    21301 gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc
    21361 ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca
    21421 atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc
    21481 gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa
    21541 gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa
    21601 gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat
    21661 catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact
    21721 gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa
    21781 gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa
    21841 taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta
    21901 tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata
    21961 gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa
    22021 tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta
    22081 gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt
    22141 atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg
    22201 tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg
    22261 gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac
    22321 aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg
    22381 agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg
    22441 gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag
    22501 ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg
    22561 gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc
    22621 tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg
    22681 catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca
    22741 tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct
    22801 cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag
    22861 tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg
    22921 aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga
    22981 gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa
    23041 aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc
    23101 ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga
    23161 tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt
    23221 cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat
    23281 tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc
    23341 tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg
    23401 tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac
    23461 agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga
    23521 agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga
    23581 aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta
    23641 tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat
    23701 gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg
    23761 tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg
    23821 gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga
    23881 agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc
    23941 agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa
    24001 agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc
    24061 gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca
    24121 tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt
    24181 tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg
    24241 taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg
    24301 tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac
    24361 ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac
    24421 gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa
    24481 aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc
    24541 atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt
    24601 ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc
    24661 cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga
    24721 aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc
    24781 tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc
    24841 aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat
    24901 catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg
    24961 tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc
    25021 agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat
    25081 cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac
    25141 tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg
    25201 atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta
    25261 ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca
    25321 tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga
    25381 gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt
    25441 ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta
    25501 ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact
    25561 gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta
    25621 ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac
    25681 tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg
    25741 gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg
    25801 accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac
    25861 tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc
    25921 caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac
    25981 taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg
    26041 atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt
    26101 cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg
    26161 gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg
    26221 caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct
    26281 ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca
    26341 tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg
    26401 aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg
    26461 aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg
    26521 gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac
    26581 acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg
    26641 aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc
    26701 cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca
    26761 aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt
    26821 ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg
    26881 tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt
    26941 caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca
    27001 aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac
    27061 ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg
    27121 aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta
    27181 gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac
    27241 atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa
    27301 gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt
    27361 gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca
    27421 gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta
    27481 atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag
    27541 ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc
    27601 ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga
    27661 acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt
    27721 gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag
    27781 gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg
    27841 atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa
    27901 gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa
    27961 gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa
    28021 acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa
    28081 catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca
    28141 aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat
    28201 cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt
    28261 attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc
    28321 gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt
    28381 gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc
    28441 tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg
    28501 cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt
    28561 aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct
    28621 agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt
    28681 gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct
    28741 ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga
    28801 tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat
    28861 ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac
    28921 aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc
    28981 tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg
    29041 gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc
    29101 aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat
    29161 ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac
    29221 gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg
    29281 attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg
    29341 aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt
    29401 gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga
    29461 gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg
    29521 tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga
    29581 atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta
    29641 attgccttga tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta
    29701 ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc
    29761 atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg
    29821 acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac
    29881 gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg
    29941 atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat
    30001 aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt
    30061 ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc
    30121 ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa
    30181 aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta
    30241 ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt
    30301 aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt
    30361 ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc
    30421 ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa
    30481 attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt
    30541 ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg
    30601 tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa
    30661 agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa
    30721 ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca
    30781 ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc
    30841 gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag
    30901 aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca
    30961 aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag
    31021 aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg
    31081 attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg
    31141 tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc
    31201 aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc
    31261 cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga
    31321 atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg
    31381 ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa
    31441 gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca
    31501 gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct
    31561 atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag
    31621 aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg
    31681 atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg
    31741 cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt
    31801 cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag
    31861 aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa
    31921 aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga
    31981 tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac
    32041 agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc
    32101 catgcaaggg agattccact actatgaagg tcattccatg caaacagtga cctatccagt
    32161 acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt
    32221 caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg
    32281 tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag
    32341 tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt
    32401 ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga
    32461 gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt
    32521 catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc
    32581 agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa
    32641 acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata
    32701 aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg
    32761 gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca
    32821 atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc
    32881 aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt
    32941 atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg
    33001 ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt
    33061 tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga
    33121 tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct
    33181 acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa
    33241 tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga
    33301 tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta
    33361 cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg
    33421 aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt
    33481 tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt
    33541 gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
    33601 ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa
    33661 aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta taggtagtat
    33721 tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc tgttggaatt
    33781 tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag ggcgcgtaat
    33841 gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg agcaatttca
    33901 tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc accaggaata
    33961 tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc gtcagtttct
    34021 tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg
    34081 tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg acgttcgttt
    34141 aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa gatcaagtta
    34201 catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc gattccagct
    34261 tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc tagatttaca
    34321 tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg acgagaaaaa
    34381 actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg aaattttcag
    34441 ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag ataagattct
    34501 atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt
    34561 aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata tttataacat
    34621 gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa
    34681 tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt
    34741 ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc
    34801 atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt
    34861 ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt
    34921 aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt
    34981 tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca
    35041 tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt
    35101 aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc
    35161 tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag
    35221 atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt
    35281 tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta
    35341 gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta
    35401 gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca
    35461 tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct
    35521 tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac
    35581 ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac
    35641 gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca
    35701 gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc
    35761 tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct
    35821 gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg
    35881 gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg
    35941 ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct
    36001 ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa
    36061 agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa
    36121 caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata
    36181 tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg
    36241 tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat
    36301 tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag
    36361 gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag
    36421 caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac
    36481 gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact
    36541 tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac
    36601 gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc
    36661 gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact
    36721 tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg agttacggat
    36781 gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa
    36841 attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa
    36901 cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt
    36961 gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg
    37021 gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct
    37081 tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc
    37141 aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac
    37201 tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg
    37261 tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt
    37321 aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat
    37381 cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta
    37441 tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat
    37501 ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa
    37561 gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga
    37621 acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga
    37681 acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt
    37741 tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga
    37801 ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac
    37861 aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga
    37921 gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa
    37981 attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga
    38041 agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt
    38101 ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc
    38161 tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa
    38221 aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt
    38281 taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt
    38341 tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg
    38401 tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat
    38461 cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag
    38521 tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc
    38581 taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga
    38641 tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg
    38701 tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa
    38761 cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg
    38821 taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac
    38881 accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc
    38941 tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt
    39001 agctggctat ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt
    39061 cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa
    39121 gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga
    39181 ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga
    39241 agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg
    39301 ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga
    39361 ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa
    39421 agctgaataa tttcttaaaa ccggatagtc ctgcaaagag attatccaaa gagactatat
    39481 ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg
    39541 tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa
    39601 aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca
    39661 cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa
    39721 cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg
    39781 aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt
    39841 tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga
    39901 cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat
    39961 cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat
    40021 ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga
    40081 atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga
    40141 aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat
    40201 cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc
    40261 aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg
    40321 tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt
    40381 tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact
    40441 acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat
    40501 cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg
    40561 tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac
    40621 aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat
    40681 ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga
    40741 aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt
    40801 tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt
    40861 tgttccgagc ttttttattt attgcaggat caggaaaaaa gacaggagta ttataaatag
    40921 aagaaacacg aaataaaaga gtcagtattt tctaaatgaa actttatgaa aacaaaatgt
    40981 tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac aaaggtgcgt
    41041 ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag aaacgagagg
    41101 ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc gtggagctat
    41161 gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa attcgtatcc
    41221 gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc gtggaaactg
    41281 caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa cgcagtcttt
    41341 acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc gaaatgcgta
    41401 cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat gctttgatga
    41461 agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata
    41521 atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg acacaaatct
    41581 tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca aacgtagtac
    41641 tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt taccaagata
    41701 tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca aacacggctc
    41761 ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag
    41821 aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt actacgaaag
    41881 gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca atggctcacg
    41941 gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac cgtgtattta
    42001 aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa aacttggaag
    42061 ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt cctggagcta
    42121 aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag gaagaaagga
    42181 ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa aacggtgaaa
    42241 tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt tacgatgcaa
    42301 tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg
    42361 ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat
    42421 ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt
    42481 acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa
    42541 aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa
    42601 aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg gttgttctag
    42661 aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt tctgtagtaa
    42721 cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa
    42781 ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg taatcaaacg
    42841 cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca cttttgaagt
    42901 ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt ttgacgttaa
    42961 agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc
    43021 aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt caaaagaaat
    43081 ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt aggagggaaa
    43141 aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat gacaggttct
    43201 gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc attgaaaaac
    43261 catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg cggacacaaa
    43321 cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc aactgttcaa
    43381 acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta tgaagatgga
    43441 gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact tgtttcaggt
    43501 ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc agttggtact
    43561 gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg ttctgctggt
    43621 acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt gaactctggt
    43681 gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg taacgaacaa
    43741 cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa acgcccaact
    43801 gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga aggtaaagct
    43861 cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg ttacaaaact
    43921 cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa taaataatta
    43981 ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag tttaaagaaa
    44041 ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag
    44101 aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt tgttggatat
    44161 accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga agacatggta
    44221 ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc tgctgacgat
    44281 aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac aaatcacatc
    44341 agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt tagtaattga
    44401 ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca caccaaacaa
    44461 atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag aaaataactt
    44521 tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag gacctacaat
    44581 gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac gtacaagtca
    44641 tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa aaagtacatc
    44701 caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat gctgaaaaag
    44761 agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca actaaacttg
    44821 ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa
    44881 ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa
    44941 gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg gaaatcaaaa
    45001 aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg
    45061 ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag
    45121 ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat
    45181 actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac gcatgggaag
    45241 aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt ggagaaattc
    45301 ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac ctaaacgtgt
    45361 aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt
    45421 agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa accgccaaat
    45481 cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag tatggattaa
    45541 aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg gttctggtaa
    45601 aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc
    45661 aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca aattaccaat
    45721 gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa
    45781 tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt
    45841 ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt attaaagaag
    45901 tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag taattatgga
    45961 aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat caaggtcgcg
    46021 tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc
    46081 ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat gaaaacaaca
    46141 cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca gctacaaaac
    46201 gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac gagattttcc
    46261 tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc
    46321 gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg
    46381 gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa
    46441 cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat
    46501 caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct gcagtgatta
    46561 ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca cgtgaactac
    46621 gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa tcttgagaca
    46681 cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa gttaaagtta
    46741 tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg aaaaaagata
    46801 aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct caagctgctc
    46861 ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg atggtggttg
    46921 actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa aaagtccgtg
    46981 tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga gggtcttact
    47041 gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg atggaaaaat
    47101 ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc aacatgggtg
    47161 ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa ttaactttga
    47221 tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga ttccgtttac
    47281 gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg tacgaattct
    47341 tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt gtaagtaaaa
    47401 aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg atcttcccag
    47461 aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt gtaacaacag
    47521 caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat
    47581 aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg
    47641 caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca cattcagttt
    47701 atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa ggtcaaattc
    47761 ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag gtaaaagatc
    47821 aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg caaacatggt
    47881 taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt
    47941 gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac aaggcgtgat
    48001 ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat
    48061 ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg
    48121 tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag aagctcgtga
    48181 aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa tctacaagga
    48241 ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa ggagtaacag
    48301 ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg actcgtactt
    48361 tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact cgtccaaacg
    48421 acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac aacatggttg
    48481 taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg taccgtgcac
    48541 aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta gaaatcacac
    48601 caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt aaaggtgcga
    48661 acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct ccagaacctt
    48721 ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg
    48781 gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg catttggctg
    48841 aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt ttcgaaacgt
    48901 atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt gattacaaaa
    48961 ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt
    49021 actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta cgctcaaatt
    49081 attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa agaaatttca
    49141 ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga acgtgcagct
    49201 gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca tggccgtgtg
    49261 caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa ggaggaatac
    49321 cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt gttgcaatta
    49381 accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct ttagttgttg
    49441 tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa gttcctgaag
    49501 caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct atggttggtt
    49561 caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta atgaaacctg
    49621 ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta gaattagctg
    49681 gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac gttgttcgcg
    49741 caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa cttcgcggca
    49801 aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat taaaagttac
    49861 tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta aagcgttagg
    49921 tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta aaggcatgat
    49981 caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa tcataactag
    50041 aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct gaaggttcac
    50101 gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa actgccggac
    50161 gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga tttgaagggg
    50221 gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag
    50281 actatgcagt cattaatctt gatgtattga accgctttga agacggagct gaagtaactc
    50341 ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc aaagtattag
    50401 ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct aaagcagctc
    50461 aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta gactattaaa
    50521 agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat ttgtgttgtt
    50581 tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt cattgcaaaa
    50641 cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa
    50701 cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg tcattcagct
    50761 tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg
    50821 taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta tccaatctgt
    50881 cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata atcctaacgt
    50941 agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg ttacttggct
    51001 aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca tttttgcagg
    51061 tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact ttataaatat
    51121 tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag tagcggtctt
    51181 gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc cgatccaata
    51241 tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag taaatgcagc
    51301 tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg ctgttttaca
    51361 agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa
    51421 ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct tcactttctt
    51481 ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa agcaaggaag
    51541 ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa gattactgat
    51601 gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc caatcattgc
    51661 tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct tactgatcgt
    51721 tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata
    51781 tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac gttctcacac
    51841 atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc gcaggtaaag
    51901 gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca acaggagata
    51961 tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag tcttatatgg
    52021 ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag cgactagctg
    52081 aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta gatcaagcaa
    52141 aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc atcgatatcc
    52201 atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc agcaattgtg
    52261 gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt gatcgttgcg
    52321 gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa aatcgtctgg
    52381 ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa ggtttgctaa
    52441 acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg
    52501 aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg tcaagttcaa
    52561 taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg atccattttg
    52621 agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag
    52681 tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc
    52741 gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca agttcttgct
    52801 actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga caaagtaacg
    52861 gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt taaataattg
    52921 tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta aaaccaatgt
    52981 gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc
    53041 caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc gtattgcagg
    53101 agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct atggtattgg
    53161 taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg ttcgcgttcg
    53221 tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat taaaagttga
    53281 aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa tcggttctta
    53341 ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga aaaacaatgc
    53401 acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat tcttaagtga
    53461 aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa
    53521 tatagaagca ggtattgcac atatccattc tacattcaat aacactatca tcatgatcac
    53581 tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt tcaaaggaag
    53641 caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa aagcagcaat
    53701 ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg gacgtgaagc
    53761 agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg acgtgactcc
    53821 agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag tgccgctcat
    53881 tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa ggggtaaaga
    53941 taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg
    54001 gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg aattccctac
    54061 gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa atcgatggtg
    54121 tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa atcatcttga
    54181 acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata
    54241 ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat gtagaaattc
    54301 ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa
    54361 cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa
    54421 ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg
    54481 agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag atttggacag
    54541 atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg actgaacatt
    54601 tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag
    54661 aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac ttgtctgttc
    54721 gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt acaaataaat
    54781 ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa gaagttaaag
    54841 caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga
    54901 gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt aaagcgatgt
    54961 tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact gaagctcgtg
    55021 cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc
    55081 atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa
    55141 atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt gcacctcgtt
    55201 acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga cgcggtgatg
    55261 gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc actttataga
    55321 tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc tcagcgctgc
    55381 tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt tttatacctt
    55441 caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa gcgagaaata
    55501 attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga agaaggagta
    55561 atcatggagc caattattga attagaaaag ataaattata aataccagcc agatgatctt
    55621 cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat cgctattatt
    55681 ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt gcttctgcca
    55741 gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat ttggacgatc
    55801 cgacagatgg ttggaatgat ttttcaaaat ccagacaatc agtttgttgg ttcgacagta
    55861 gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga aatgctagtg
    55921 cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg cgagcctgct
    55981 cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc cttacgacct
    56041 gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg tgaagaagtg
    56101 atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc tatcactcat
    56161 gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg cgaacttgtc
    56221 cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga cttaggtcta
    56281 gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt tgatgtgccc
    56341 agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt tttgaacaag
    56401 tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt gacatcaata
    56461 tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc gggaaatcaa
    56521 ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc catattggag
    56581 aacgagatat tcagccggat acggataata agaatttaaa gcccatccga aaaaaagttg
    56641 gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca aaagacattg
    56701 ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa
    56761 cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt gaactttcag
    56821 gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg
    56881 tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg atggagatgt
    56941 tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat ttaatggatg
    57001 atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc gttaatagcg
    57061 gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa ctaggcgtac
    57121 ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt gctaccttgc
    57181 ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga aatgcccatg
    57241 atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca tcgattggat
    57301 ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt agcaaataat
    57361 tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt gtcaaaagta
    57421 gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct cttcactgta
    57481 gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg tgttttccat
    57541 atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt gttgatcatt
    57601 tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga tgcgattgag
    57661 tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc attgatgctg
    57721 tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat catgaacgct
    57781 cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc
    57841 attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga tcttgcgact
    57901 gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat
    57961 tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt gctactgatc
    58021 tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct gatctgtttg
    58081 caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca gactaaggtc
    58141 acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga aaaatcatta
    58201 ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca aagcaatcat
    58261 tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc gtactgttca
    58321 agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg
    58381 ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt ttgattatcc
    58441 agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac cagaggatat
    58501 tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt tggtcaaaga
    58561 aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc gacgccatta
    58621 tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt
    58681 attagggact cacgatttca cctctttttg tgcatcagga agctcgatag aggataaaat
    58741 acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat tgttgtttac
    58801 atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa cgcttttgaa
    58861 aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga aaaaagatcg
    58921 taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag tgaaatatga
    58981 ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt acgaaaatta
    59041 cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct ggaacaccgt
    59101 ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca atttcaaaaa
    59161 acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca
    59221 tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc
    59281 ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc ttcttctgtg
    59341 tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg
    59401 gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa ggattgcacc
    59461 tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg ctggaacttt
    59521 tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat caattttagc
    59581 tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag cggtatttac
    59641 agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt tgatctgatt
    59701 cattgcggct tgataggcta caacatctag accaacgact ttaggctcca tgaaacttat
    59761 ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc
    59821 aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac cattgacagt
    59881 gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc
    59941 ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt tatctgctgt
    60001 ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa
    60061 agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat tgatcaagtt
    60121 tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc
    60181 agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc cgctttgacc
    60241 agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt tatatttgat
    60301 tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca aatttgtccc
    60361 tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc ttatagaaga
    60421 aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata ctggatccag
    60481 attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa gagctttcaa
    60541 gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat caagaatgcc
    60601 atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata ataacaatat
    60661 actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa aagcataaat
    60721 ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt agcttccttc
    60781 aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga ttcctaacaa
    60841 cagcccacca tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca ggagcactaa
    60901 gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca taccatgcca
    60961 gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa gactgccgta
    61021 caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac agaggactaa
    61081 tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt cataaacacc
    61141 aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca acgtagtgat
    61201 ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata caatgatcat
    61261 tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt ttttatattc
    61321 tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc caatcataaa
    61381 acccatataa aaactggata aaaacaagag taaataactg ataaataaaa gtttcggccg
    61441 tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa caaatgcatc
    61501 cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt aactgacgct
    61561 aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa
    61621 tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca tcatctggtt
    61681 tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa
    61741 ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc caagaagaac
    61801 atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact
    61861 tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa tcaaaaacgg
    61921 aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact ttaaattttt
    61981 tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc atatccttgt
    62041 ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga ataaataact
    62101 tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca
    62161 cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt
    62221 caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt
    62281 aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt
    62341 tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca
    62401 aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct
    62461 cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa
    62521 ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt
    62581 ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag
    62641 aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa
    62701 cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg
    62761 cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta
    62821 ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg
    62881 gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc
    62941 gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt
    63001 cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg
    63061 taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa
    63121 gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg
    63181 tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat
    63241 ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag
    63301 cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt
    63361 ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg
    63421 atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag
    63481 tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa
    63541 aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt
    63601 tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag
    63661 ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag
    63721 cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc
    63781 atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa
    63841 tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc
    63901 gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca
    63961 aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt
    64021 cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg
    64081 atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag
    64141 cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct aattatgtta
    64201 tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag
    64261 taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc
    64321 aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag
    64381 gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga
    64441 tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct
    64501 caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc
    64561 tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg
    64621 aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg
    64681 aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag
    64741 atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc
    64801 taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa
    64861 gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac
    64921 tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac
    64981 gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct
    65041 aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt
    65101 gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga
    65161 tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga
    65221 ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat
    65281 tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg
    65341 tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga
    65401 agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt
    65461 aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt
    65521 ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc
    65581 agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa
    65641 gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct
    65701 tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt
    65761 ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt
    65821 gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa
    65881 tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct
    65941 tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga
    66001 tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt
    66061 gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc
    66121 gactccgatt cctagaacat tagctattac agcatatggt gagatggatg tatctatcat
    66181 tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt
    66241 ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat
    66301 ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga
    66361 aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa
    66421 gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt
    66481 ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat
    66541 ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg
    66601 aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg
    66661 gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt
    66721 acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc
    66781 tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg
    66841 gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat
    66901 agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac
    66961 ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc
    67021 tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt
    67081 actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat
    67141 tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa
    67201 aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt
    67261 attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat
    67321 caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc
    67381 cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca
    67441 atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt
    67501 cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta
    67561 tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct
    67621 actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa
    67681 atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga
    67741 agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc
    67801 agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat
    67861 taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac
    67921 aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata
    67981 gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat
    68041 gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa
    68101 agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga
    68161 tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa
    68221 ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg
    68281 aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta
    68341 aataattt
//
LOCUS       DAZBTH010000005        67458 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000005 DAZBTH010000000
VERSION     DAZBTH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.005"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHGNW_000312"
     CDS             complement(105..629)
                     /locus_tag="ACHGNW_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN6924663.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGNW_000313"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGNW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN6924664.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGNW_000314"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGNW_000314"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN6924665.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHGNW_000315"
     CDS             complement(2351..3031)
                     /locus_tag="ACHGNW_000315"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6924666.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHGNW_000316"
     CDS             3157..4026
                     /locus_tag="ACHGNW_000316"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6924667.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHGNW_000317"
     CDS             4052..4567
                     /locus_tag="ACHGNW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN6924668.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHGNW_000318"
     CDS             complement(4614..5177)
                     /locus_tag="ACHGNW_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924669.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHGNW_000319"
     CDS             complement(5232..5411)
                     /locus_tag="ACHGNW_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924670.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHGNW_000320"
     CDS             complement(5488..5997)
                     /locus_tag="ACHGNW_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924671.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHGNW_000321"
     CDS             complement(6093..6473)
                     /locus_tag="ACHGNW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6924672.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGNW_000322"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGNW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN6924673.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHGNW_000323"
     CDS             complement(7736..9013)
                     /locus_tag="ACHGNW_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924674.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHGNW_000324"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHGNW_000324"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN6924675.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHGNW_000325"
     CDS             complement(9935..10879)
                     /locus_tag="ACHGNW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN6924676.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHGNW_000326"
     CDS             complement(10881..11567)
                     /locus_tag="ACHGNW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN6924677.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="ACHGNW_000327"
     CDS             12041..12361
                     /locus_tag="ACHGNW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN6924678.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="ACHGNW_000328"
     CDS             12515..13420
                     /locus_tag="ACHGNW_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924679.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="ACHGNW_000329"
     CDS             13417..14190
                     /locus_tag="ACHGNW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6924680.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="ACHGNW_000330"
     CDS             complement(14371..15795)
                     /locus_tag="ACHGNW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN6924681.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHGNW_000331"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHGNW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN6924682.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="ACHGNW_000332"
     CDS             complement(16517..18499)
                     /locus_tag="ACHGNW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6924683.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHGNW_000333"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHGNW_000333"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6924684.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="ACHGNW_000334"
     CDS             complement(19412..20473)
                     /locus_tag="ACHGNW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6924685.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="ACHGNW_000335"
     CDS             complement(20684..21211)
                     /locus_tag="ACHGNW_000335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6924686.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="ACHGNW_000336"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="ACHGNW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6924687.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="ACHGNW_000337"
     CDS             complement(23038..24159)
                     /locus_tag="ACHGNW_000337"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6924688.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="ACHGNW_000338"
     CDS             24285..25226
                     /locus_tag="ACHGNW_000338"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6924689.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="ACHGNW_000339"
     CDS             complement(25376..26272)
                     /locus_tag="ACHGNW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN6924690.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="ACHGNW_000340"
     CDS             26541..27881
                     /locus_tag="ACHGNW_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6924691.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="ACHGNW_000341"
     CDS             28099..28542
                     /locus_tag="ACHGNW_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN6924692.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="ACHGNW_000342"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="ACHGNW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6924693.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="ACHGNW_000343"
     CDS             complement(30867..32090)
                     /locus_tag="ACHGNW_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6924694.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="ACHGNW_000344"
     CDS             complement(32121..32960)
                     /locus_tag="ACHGNW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6924695.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="ACHGNW_000345"
     CDS             complement(32977..33657)
                     /locus_tag="ACHGNW_000345"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6924696.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="ACHGNW_000346"
     CDS             complement(33659..34732)
                     /locus_tag="ACHGNW_000346"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6924697.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="ACHGNW_000347"
     CDS             complement(35379..36050)
                     /locus_tag="ACHGNW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6924698.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38785)
                     /gene="mgtA"
                     /locus_tag="ACHGNW_000348"
     CDS             complement(36137..38785)
                     /gene="mgtA"
                     /locus_tag="ACHGNW_000348"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN6924699.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(39127..39301)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="ACHGNW_000349"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="ACHGNW_000349"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN6924700.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="ACHGNW_000350"
     CDS             complement(39933..40799)
                     /locus_tag="ACHGNW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN6924701.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="ACHGNW_000351"
     CDS             complement(40959..43052)
                     /locus_tag="ACHGNW_000351"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6924702.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="ACHGNW_000352"
     CDS             complement(43801..44424)
                     /locus_tag="ACHGNW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924703.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="ACHGNW_000353"
     CDS             complement(44583..45980)
                     /locus_tag="ACHGNW_000353"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6924704.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="ACHGNW_000354"
     CDS             complement(46012..46983)
                     /locus_tag="ACHGNW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6924705.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="ACHGNW_000355"
     CDS             47357..47782
                     /locus_tag="ACHGNW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924706.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="ACHGNW_000356"
     CDS             complement(48270..48971)
                     /locus_tag="ACHGNW_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6924707.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="ACHGNW_000357"
     CDS             complement(48987..49427)
                     /locus_tag="ACHGNW_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6924708.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="ACHGNW_000358"
     CDS             complement(49427..50002)
                     /locus_tag="ACHGNW_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN6924709.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            50243..50452
                     /locus_tag="ACHGNW_000359"
     CDS             50243..50452
                     /locus_tag="ACHGNW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN6924710.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(50850..51362)
                     /locus_tag="ACHGNW_000360"
                     /pseudo
     CDS             complement(50850..51362)
                     /locus_tag="ACHGNW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51488..52189)
                     /locus_tag="ACHGNW_000361"
     CDS             complement(51488..52189)
                     /locus_tag="ACHGNW_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6924711.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52297..53740)
                     /locus_tag="ACHGNW_000362"
                     /pseudo
     CDS             complement(52297..53740)
                     /locus_tag="ACHGNW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(53754..55619)
                     /locus_tag="ACHGNW_000363"
     CDS             complement(53754..55619)
                     /locus_tag="ACHGNW_000363"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6924712.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55740..56591)
                     /gene="licT"
                     /locus_tag="ACHGNW_000364"
     CDS             complement(55740..56591)
                     /gene="licT"
                     /locus_tag="ACHGNW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN6924713.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56901..57614)
                     /locus_tag="ACHGNW_000365"
     CDS             complement(56901..57614)
                     /locus_tag="ACHGNW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN6924714.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57682..58008)
                     /locus_tag="ACHGNW_000366"
     CDS             complement(57682..58008)
                     /locus_tag="ACHGNW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6924715.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58131..58358)
                     /locus_tag="ACHGNW_000367"
     CDS             complement(58131..58358)
                     /locus_tag="ACHGNW_000367"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6924716.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58680..59933)
                     /locus_tag="ACHGNW_000368"
     CDS             complement(58680..59933)
                     /locus_tag="ACHGNW_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6924717.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59994..60734
                     /locus_tag="ACHGNW_000369"
     CDS             59994..60734
                     /locus_tag="ACHGNW_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6924718.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60820..61578)
                     /locus_tag="ACHGNW_000370"
     CDS             complement(60820..61578)
                     /locus_tag="ACHGNW_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6924719.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61575..62039)
                     /locus_tag="ACHGNW_000371"
                     /pseudo
     CDS             complement(61575..62039)
                     /locus_tag="ACHGNW_000371"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(62058..62555)
                     /locus_tag="ACHGNW_000372"
     CDS             complement(62058..62555)
                     /locus_tag="ACHGNW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN6924720.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(62623..65790)
                     /locus_tag="ACHGNW_000373"
     CDS             complement(62623..65790)
                     /locus_tag="ACHGNW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN6924721.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66014..66898)
                     /locus_tag="ACHGNW_000374"
     CDS             complement(66014..66898)
                     /locus_tag="ACHGNW_000374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6924722.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66993..67430)
                     /locus_tag="ACHGNW_000375"
     CDS             complement(66993..67430)
                     /locus_tag="ACHGNW_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924723.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        19362 a        14210 c        11654 g        22232 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
    50101 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
    50161 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
    50221 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
    50281 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
    50341 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
    50401 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
    50461 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
    50521 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
    50581 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
    50641 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
    50701 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
    50761 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
    50821 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
    50881 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
    50941 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
    51001 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
    51061 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
    51121 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
    51181 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
    51241 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
    51301 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
    51361 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
    51421 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    51481 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
    51541 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
    51601 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
    51661 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
    51721 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
    51781 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
    51841 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
    51901 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
    51961 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
    52021 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
    52081 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
    52141 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
    52201 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
    52261 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
    52321 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
    52381 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
    52441 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
    52501 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
    52561 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
    52621 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
    52681 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
    52741 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
    52801 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
    52861 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
    52921 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
    52981 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
    53041 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
    53101 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
    53161 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
    53221 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
    53281 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
    53341 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
    53401 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
    53461 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
    53521 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
    53581 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
    53641 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
    53701 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    53761 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    53821 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    53881 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    53941 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    54001 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    54061 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    54121 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    54181 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt
    54241 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    54301 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    54361 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    54421 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    54481 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    54541 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    54601 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    54661 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    54721 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    54781 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    54841 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    54901 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    54961 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    55021 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    55081 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    55141 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    55201 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    55261 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    55321 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    55381 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    55441 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    55501 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    55561 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    55621 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    55681 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    55741 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    55801 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    55861 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    55921 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    55981 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    56041 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    56101 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    56161 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    56221 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    56281 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    56341 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    56401 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg
    56461 accgcttctg gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata
    56521 caaacgactt cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta
    56581 tcaattttca ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact
    56641 gtttcttagc tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa
    56701 atcatcatac catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact
    56761 ggaacacaag tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt
    56821 tttttcagca aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta
    56881 acgacttatg tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata
    56941 tcatctgtcg catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc
    57001 gtgacggcgt ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca
    57061 ttcagtaatt gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac
    57121 acattttcta aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta
    57181 aacagcaatt cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt
    57241 tcttgatctg gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg
    57301 aaaaccggta aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct
    57361 gttcctctca tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag
    57421 tcaaaactga catctttatc gttattcttt ttcagatctt cttttgttac attcgctata
    57481 gcatatttat ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa
    57541 gctagtccta ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt
    57601 cctttttttt tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc
    57661 caacagccga atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt
    57721 ttctacagga tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc
    57781 caggtgaagg ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt
    57841 gattcgttct aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa
    57901 caattcagat gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg
    57961 ccaattacgt tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc
    58021 ttccaatcac ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc
    58081 ttttaaaaaa actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt
    58141 aggtagccac catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg
    58201 atacgttctt cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct
    58261 tcggtgagcg agtactctac tttaggcggg acttggttat aggcttttcg atggatactt
    58321 tcatcttgtt ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt
    58381 tgcttgcttc tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca
    58441 aagcagttag cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc
    58501 atgaagtata ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta
    58561 catagcaaaa gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg
    58621 ctgtgttgtt gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt
    58681 cagtaggctg atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc
    58741 tgttttgaaa attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca
    58801 ctgtatagac ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat
    58861 agcgtcaaat tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct
    58921 tccagcagat tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa
    58981 ataaaaatag ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg
    59041 acaggaataa ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa
    59101 aagacacccc tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca
    59161 aaaatagctg cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt
    59221 tcctgaagag taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct
    59281 agatgatggt aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt
    59341 tcttttactt ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact
    59401 atcgacaaaa gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc
    59461 aagtaaatca cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga
    59521 taagtaaaaa gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag
    59581 aaaataaatg cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa
    59641 taatccatca gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta
    59701 ctgatcaaac agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat
    59761 aagccaacta ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc
    59821 aaagctatac aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata
    59881 cctaaatcta aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc
    59941 ctccattgtt ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa
    60001 agtattttat catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca
    60061 atcatcatat cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc
    60121 agttgggcac tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac
    60181 tcacaggtat tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt
    60241 ttttcaccgc ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg
    60301 gatatatcag aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca
    60361 attggaaaga acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa
    60421 gtctcgtcct gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc
    60481 aagttatggt caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga
    60541 agacagagtt agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat
    60601 cgcaattgca gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct
    60661 tactcgttgg tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa
    60721 ctgcttcgag ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc
    60781 cttgaatcgg ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg
    60841 agcatctgtt aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac
    60901 aggaacaaat aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat
    60961 ccatgtgcca atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg
    61021 gatttcgatt ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt
    61081 tgcgctattt cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt
    61141 atcagatgtt gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt
    61201 tccgctttta tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc
    61261 cgttccgctt tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa
    61321 atcaagatct ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc
    61381 aggtgtaaaa ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc
    61441 aggatttgca gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt
    61501 attctcagca aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa
    61561 tgcaattgtt tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat
    61621 aagccaataa gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga
    61681 ttttttaaat caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa
    61741 tcggtatgga gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt
    61801 cccggagcta atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct
    61861 acatctattt tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt
    61921 tcaaccgatg tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta
    61981 atcacatttc ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa
    62041 gactcttttt cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca
    62101 ccaactgtac ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg
    62161 accacaccag ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt
    62221 tgaggaggaa cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat
    62281 gtttcatcaa atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta
    62341 gcggagtgga tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag
    62401 gggatttcct gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca
    62461 atctgattag ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt
    62521 gtctgccaaa ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga
    62581 cttttattcc atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc
    62641 tgctaatgtc catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa
    62701 gacaagttta aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt
    62761 tgaattttgt cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc
    62821 tttcttgaaa tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga
    62881 taattttatc gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt
    62941 ttcctgtata tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc
    63001 aaaatcgagt ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc
    63061 aggtgcaaaa ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc
    63121 aatttcatcg ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg
    63181 gtcgtttaat acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag
    63241 aacaaattcc cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa
    63301 tgaaaccaag gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa
    63361 tgtggtactt aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc
    63421 cagttctgtc acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc
    63481 ctccgttttt aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc
    63541 ttctccccat gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc
    63601 agtaatttct acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac
    63661 ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc
    63721 atagattttt ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt
    63781 attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt
    63841 taaacctgca ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc
    63901 tgtcatatca gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt
    63961 tcgccataaa gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa
    64021 tacccagccg cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata
    64081 ggtaggagat tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc
    64141 ccctgttttc ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat
    64201 atctccagtt gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc
    64261 tccagtgact gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa
    64321 ttggatcgct tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt
    64381 tcttaccctc aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg
    64441 aactgcgctg gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac
    64501 gatcaaagaa aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc
    64561 tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc
    64621 ttgcaagcga aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac
    64681 agtgactttc gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat
    64741 attgatcttt gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt
    64801 accaggtagc gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc
    64861 aagagagacc tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt
    64921 ctcatctcct gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact
    64981 accattgatc acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt
    65041 cagtccttcc ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt
    65101 tgcacgagga gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt
    65161 aaattttatt tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat
    65221 gataatggca tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt
    65281 aaaattctgg aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa
    65341 attgtgagcg aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc
    65401 taaagtcaaa ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc
    65461 cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc
    65521 cgcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc
    65581 ttcttgcctt ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga
    65641 ttcttctgaa aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt
    65701 tttctctaca tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga
    65761 cgcaatcagc ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa
    65821 ttttatttta tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt
    65881 tatggaaaaa tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac
    65941 aaaagtcttg tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt
    66001 cacaacctcc attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag
    66061 gtaatttatc cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg
    66121 acacaactaa ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt
    66181 ttaataggtc tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca
    66241 aatggagctt accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg
    66301 agttagttag tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt
    66361 ctacgtgaaa cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag
    66421 ctagttttcc aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag
    66481 agccaattgc gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct
    66541 tgactgattc aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg
    66601 ctcgagccaa gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt
    66661 gttcagcgat tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac
    66721 tatgataccc ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg
    66781 tatgcaagac gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa
    66841 attcctttgc ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca
    66901 ctttctttcc cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg
    66961 ctgtgatgaa gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat
    67021 actatgaaac tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat
    67081 tgtataccta ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga
    67141 atgtaatagc gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca
    67201 aatgcataac tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat
    67261 gcgacaatcc ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat
    67321 tttatataca aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg
    67381 tatacgtgaa taacttctag aaataaaaga aatttgcaaa accactccat ttatctactt
    67441 ctacgtgcat tttttgat
//
LOCUS       DAZBTH010000006        60192 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000006 DAZBTH010000000
VERSION     DAZBTH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.006"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHGNW_000376"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHGNW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN6924724.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHGNW_000377"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHGNW_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN6924725.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHGNW_000378"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHGNW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN6924726.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHGNW_000379"
     CDS             1642..2274
                     /locus_tag="ACHGNW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6924727.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHGNW_000380"
     CDS             complement(2276..3181)
                     /locus_tag="ACHGNW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6924728.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHGNW_000381"
     CDS             complement(3266..3925)
                     /locus_tag="ACHGNW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6924729.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHGNW_000382"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHGNW_000382"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN6924730.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHGNW_000383"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHGNW_000383"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN6924731.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHGNW_000384"
     CDS             6168..7253
                     /locus_tag="ACHGNW_000384"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN6924732.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHGNW_000385"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHGNW_000385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN6924733.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHGNW_000386"
     CDS             complement(8563..9366)
                     /locus_tag="ACHGNW_000386"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN6924734.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHGNW_000387"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHGNW_000387"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN6924735.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHGNW_000388"
     CDS             complement(10244..11251)
                     /locus_tag="ACHGNW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN6924736.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHGNW_000389"
     CDS             complement(11258..11902)
                     /locus_tag="ACHGNW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN6924737.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHGNW_000390"
     CDS             complement(12002..12760)
                     /locus_tag="ACHGNW_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN6924738.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHGNW_000391"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHGNW_000391"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN6924739.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHGNW_000392"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHGNW_000392"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN6924740.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHGNW_000393"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHGNW_000393"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN6924741.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHGNW_000394"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHGNW_000394"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN6924742.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHGNW_000395"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHGNW_000395"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN6924743.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHGNW_000396"
     CDS             18158..19027
                     /locus_tag="ACHGNW_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6924744.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHGNW_000397"
     CDS             complement(19153..20052)
                     /locus_tag="ACHGNW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6924745.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHGNW_000398"
     CDS             complement(20217..21041)
                     /locus_tag="ACHGNW_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6924746.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHGNW_000399"
     CDS             complement(21052..22347)
                     /locus_tag="ACHGNW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN6924747.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHGNW_000400"
     CDS             complement(22363..22842)
                     /locus_tag="ACHGNW_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN6924748.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHGNW_000401"
     CDS             complement(22848..23876)
                     /locus_tag="ACHGNW_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN6924749.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHGNW_000402"
     CDS             complement(23893..24999)
                     /locus_tag="ACHGNW_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6924750.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHGNW_000403"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHGNW_000403"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN6924751.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHGNW_000404"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHGNW_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN6924752.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHGNW_000405"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHGNW_000405"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN6924753.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ACHGNW_000406"
     CDS             29970..30668
                     /locus_tag="ACHGNW_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6924754.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ACHGNW_000407"
     CDS             complement(30836..33475)
                     /locus_tag="ACHGNW_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6924755.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHGNW_000408"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHGNW_000408"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN6924756.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ACHGNW_000409"
     CDS             35318..36493
                     /locus_tag="ACHGNW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6924757.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ACHGNW_000410"
     CDS             36497..37138
                     /locus_tag="ACHGNW_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924758.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ACHGNW_000411"
     CDS             37135..38052
                     /locus_tag="ACHGNW_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6924759.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ACHGNW_000412"
     CDS             38049..38720
                     /locus_tag="ACHGNW_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924760.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ACHGNW_000413"
     CDS             complement(39261..39749)
                     /locus_tag="ACHGNW_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN6924761.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ACHGNW_000414"
     CDS             complement(39950..40216)
                     /locus_tag="ACHGNW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6924762.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ACHGNW_000415"
     CDS             complement(40216..40479)
                     /locus_tag="ACHGNW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6924763.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41000..41140
                     /locus_tag="ACHGNW_000416"
                     /pseudo
     CDS             41000..41140
                     /locus_tag="ACHGNW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41282..43105)
                     /locus_tag="ACHGNW_000417"
     CDS             complement(41282..43105)
                     /locus_tag="ACHGNW_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6924764.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ACHGNW_000418"
     CDS             complement(43703..46408)
                     /locus_tag="ACHGNW_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN6924765.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ACHGNW_000419"
     CDS             complement(46714..47661)
                     /locus_tag="ACHGNW_000419"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN6924766.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ACHGNW_000420"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ACHGNW_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6924767.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ACHGNW_000421"
     CDS             complement(48296..48658)
                     /locus_tag="ACHGNW_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6924768.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACHGNW_000422"
     CDS             complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACHGNW_000422"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN6924769.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACHGNW_000423"
     CDS             complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACHGNW_000423"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN6924770.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ACHGNW_000424"
     CDS             complement(50286..51797)
                     /locus_tag="ACHGNW_000424"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6924771.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /gene="murF"
                     /locus_tag="ACHGNW_000425"
     CDS             complement(52119..53471)
                     /gene="murF"
                     /locus_tag="ACHGNW_000425"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN6924772.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ACHGNW_000426"
     CDS             complement(53624..54700)
                     /locus_tag="ACHGNW_000426"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN6924773.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ACHGNW_000427"
     CDS             54896..56641
                     /locus_tag="ACHGNW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN6924774.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ACHGNW_000428"
     CDS             complement(56921..57358)
                     /locus_tag="ACHGNW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN6924775.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ACHGNW_000429"
     CDS             complement(57342..58715)
                     /locus_tag="ACHGNW_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924776.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ACHGNW_000430"
     CDS             complement(59037..59525)
                     /locus_tag="ACHGNW_000430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924777.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ACHGNW_000431"
     CDS             complement(59547..60149)
                     /locus_tag="ACHGNW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6924778.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DAZBTH010000007        58386 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000007 DAZBTH010000000
VERSION     DAZBTH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.007"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    253..458
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            570..1826
                     /gene="tyrS"
                     /locus_tag="ACHGNW_000432"
     CDS             570..1826
                     /gene="tyrS"
                     /locus_tag="ACHGNW_000432"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN6924779.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2117..3994
                     /gene="tdc"
                     /locus_tag="ACHGNW_000433"
     CDS             2117..3994
                     /gene="tdc"
                     /locus_tag="ACHGNW_000433"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6924780.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4237..5658
                     /gene="tyrP"
                     /locus_tag="ACHGNW_000434"
     CDS             4237..5658
                     /gene="tyrP"
                     /locus_tag="ACHGNW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN6924781.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5768..7135
                     /gene="nhaC"
                     /locus_tag="ACHGNW_000435"
     CDS             5768..7135
                     /gene="nhaC"
                     /locus_tag="ACHGNW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6924782.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7416..8435)
                     /locus_tag="ACHGNW_000436"
     CDS             complement(7416..8435)
                     /locus_tag="ACHGNW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6924783.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8457..9464)
                     /locus_tag="ACHGNW_000437"
     CDS             complement(8457..9464)
                     /locus_tag="ACHGNW_000437"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6924784.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9457..10122)
                     /gene="pgmB"
                     /locus_tag="ACHGNW_000438"
     CDS             complement(9457..10122)
                     /gene="pgmB"
                     /locus_tag="ACHGNW_000438"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6924785.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(10115..12409)
                     /locus_tag="ACHGNW_000439"
     CDS             complement(10115..12409)
                     /locus_tag="ACHGNW_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN6924786.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12669..14834
                     /locus_tag="ACHGNW_000440"
     CDS             12669..14834
                     /locus_tag="ACHGNW_000440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN6924787.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15138..15971
                     /locus_tag="ACHGNW_000441"
     CDS             15138..15971
                     /locus_tag="ACHGNW_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN6924788.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16073..17773)
                     /locus_tag="ACHGNW_000442"
     CDS             complement(16073..17773)
                     /locus_tag="ACHGNW_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924789.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            18280..19440
                     /locus_tag="ACHGNW_000443"
     CDS             18280..19440
                     /locus_tag="ACHGNW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6924790.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19456..19899
                     /locus_tag="ACHGNW_000444"
     CDS             19456..19899
                     /locus_tag="ACHGNW_000444"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924791.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20382..20912)
                     /locus_tag="ACHGNW_000445"
     CDS             complement(20382..20912)
                     /locus_tag="ACHGNW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN6924792.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            21017..21568
                     /locus_tag="ACHGNW_000446"
     CDS             21017..21568
                     /locus_tag="ACHGNW_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6924793.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21721..22194)
                     /locus_tag="ACHGNW_000447"
     CDS             complement(21721..22194)
                     /locus_tag="ACHGNW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6924794.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22332..23804
                     /locus_tag="ACHGNW_000448"
     CDS             22332..23804
                     /locus_tag="ACHGNW_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6924795.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23801..24511
                     /locus_tag="ACHGNW_000449"
     CDS             23801..24511
                     /locus_tag="ACHGNW_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN6924796.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            24863..25147
                     /locus_tag="ACHGNW_000450"
     CDS             24863..25147
                     /locus_tag="ACHGNW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN6924797.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(25428..25847)
                     /locus_tag="ACHGNW_000451"
     CDS             complement(25428..25847)
                     /locus_tag="ACHGNW_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN6924798.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(25849..26433)
                     /locus_tag="ACHGNW_000452"
     CDS             complement(25849..26433)
                     /locus_tag="ACHGNW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN6924799.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(26480..27955)
                     /locus_tag="ACHGNW_000453"
     CDS             complement(26480..27955)
                     /locus_tag="ACHGNW_000453"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN6924800.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            28136..28447
                     /locus_tag="ACHGNW_000454"
     CDS             28136..28447
                     /locus_tag="ACHGNW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924801.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(28562..30898)
                     /locus_tag="ACHGNW_000455"
     CDS             complement(28562..30898)
                     /locus_tag="ACHGNW_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN6924802.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            31040..31708
                     /locus_tag="ACHGNW_000456"
     CDS             31040..31708
                     /locus_tag="ACHGNW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6924803.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            31711..32754
                     /locus_tag="ACHGNW_000457"
     CDS             31711..32754
                     /locus_tag="ACHGNW_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6924804.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(32991..33872)
                     /locus_tag="ACHGNW_000458"
     CDS             complement(32991..33872)
                     /locus_tag="ACHGNW_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6924805.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            34031..34759
                     /locus_tag="ACHGNW_000459"
     CDS             34031..34759
                     /locus_tag="ACHGNW_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN6924806.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            34915..35232
                     /locus_tag="ACHGNW_000460"
     CDS             34915..35232
                     /locus_tag="ACHGNW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6924807.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            35225..35833
                     /locus_tag="ACHGNW_000461"
     CDS             35225..35833
                     /locus_tag="ACHGNW_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN6924808.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            35888..36679
                     /locus_tag="ACHGNW_000462"
     CDS             35888..36679
                     /locus_tag="ACHGNW_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN6924809.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            36817..37329
                     /locus_tag="ACHGNW_000463"
     CDS             36817..37329
                     /locus_tag="ACHGNW_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN6924810.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            37355..38227
                     /locus_tag="ACHGNW_000464"
     CDS             37355..38227
                     /locus_tag="ACHGNW_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6924811.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            38403..38765
                     /locus_tag="ACHGNW_000465"
     CDS             38403..38765
                     /locus_tag="ACHGNW_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6924812.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            38774..39526
                     /locus_tag="ACHGNW_000466"
     CDS             38774..39526
                     /locus_tag="ACHGNW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924813.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            39540..41342
                     /locus_tag="ACHGNW_000467"
     CDS             39540..41342
                     /locus_tag="ACHGNW_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924814.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            41619..42659
                     /locus_tag="ACHGNW_000468"
     CDS             41619..42659
                     /locus_tag="ACHGNW_000468"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6924815.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(42711..44855)
                     /locus_tag="ACHGNW_000469"
     CDS             complement(42711..44855)
                     /locus_tag="ACHGNW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6924816.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(45212..46585)
                     /locus_tag="ACHGNW_000470"
     CDS             complement(45212..46585)
                     /locus_tag="ACHGNW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6924817.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(46585..46980)
                     /gene="rbsD"
                     /locus_tag="ACHGNW_000471"
     CDS             complement(46585..46980)
                     /gene="rbsD"
                     /locus_tag="ACHGNW_000471"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN6924818.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(47008..47925)
                     /gene="rbsK"
                     /locus_tag="ACHGNW_000472"
     CDS             complement(47008..47925)
                     /gene="rbsK"
                     /locus_tag="ACHGNW_000472"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN6924819.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(48033..49046)
                     /locus_tag="ACHGNW_000473"
     CDS             complement(48033..49046)
                     /locus_tag="ACHGNW_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN6924820.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(49127..49726)
                     /locus_tag="ACHGNW_000474"
     CDS             complement(49127..49726)
                     /locus_tag="ACHGNW_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6924821.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            49912..50613
                     /locus_tag="ACHGNW_000475"
     CDS             49912..50613
                     /locus_tag="ACHGNW_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924822.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            50623..53334
                     /locus_tag="ACHGNW_000476"
     CDS             50623..53334
                     /locus_tag="ACHGNW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN6924823.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            53731..55359
                     /gene="treC"
                     /locus_tag="ACHGNW_000477"
     CDS             53731..55359
                     /gene="treC"
                     /locus_tag="ACHGNW_000477"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN6924824.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(55419..56600)
                     /locus_tag="ACHGNW_000478"
     CDS             complement(55419..56600)
                     /locus_tag="ACHGNW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6924825.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            56852..58375
                     /locus_tag="ACHGNW_000479"
     CDS             56852..58375
                     /locus_tag="ACHGNW_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN6924826.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        18372 a        10480 c        11966 g        17568 t
ORIGIN      
        1 ccttgctttt tcattttttt acttgtccca ttccttattg taacgaagaa agaataagag
       61 gcaaagtaaa gtgacttctc gataaattgc agaaaaaata cagaaaaagt tatttctttt
      121 aacaataatt gaaaataaaa tgttcggaat tagagcggat tcaaaaaaga aaaaatgagg
      181 aatttaaaaa aaatatcctt tttctgttga caaagaaaaa gttgcgctct atgatagaca
      241 catattaata ataacaagac gaagatcgaa acgaagtagt tgttaaagcc aatgtttcag
      301 aaagctggtg gtcgctgcaa accagtacat gctttaaagc gaattacatt tcaggagtct
      361 ttggcagtga ttgccacgcg acagcgttaa atgttcgaga ggtatgttgg atatctagac
      421 atacaaattg ggtggtaccg cgagatttcg tcccttggct tatatgccaa gggacttttt
      481 ttatttccta tcctatcctg aagaaataat taattacaca tgctagcagg ttcctgcttt
      541 cataaaaaaa ctacacggag gtcagtaaaa tgaatattat tgatgaatta acttggcgcg
      601 acgccattaa tcagcaaaca aacgaagaaa gacttcgcga gctcgttgaa gaaaagagca
      661 tttcacttta ttgcggcgtt gatccaactg gtgattcgat gcatatcgga catttgattc
      721 catttatgat gatgaaaaga ttccaattgg caggccatca tccatatatt ctgattggcg
      781 gcggaacagg aacgatcggc gacccaagcg gccgcaaaac agaacgtgtg ttgcaaacga
      841 tggaacaagt gcaacataac gtggacgcac tttccaatca aatgagaaaa ttgtttggta
      901 aagatgcaaa tatcacattt gtgaacaact acgactggtt gtcaaaaatc tctttacttg
      961 aatttttgag agactacggt aaaaacttca acatcaacac gatgttagca aaagatatcg
     1021 ttgccagccg tttggaagta ggaatctcat ttacagagtt tacttaccaa attctgcaat
     1081 ctatcgactt tttgcatctg cataaaacat acgatgtgca attgcaaatc ggtggagcag
     1141 accaatgggg aaatatcaca gctggattag acctaatccg aaaattagaa ggaccagaag
     1201 tagaagcgtt cggtttaacg atcccattga tgctgaaggc tgacggaacg aaattcggaa
     1261 aaactgcagg cggtgccgtt tggcttgatc cgaagaaaac ttcaccattt gagttctacc
     1321 aattctggtt gaatcaagat gatcgcgatg tagtgaaata cttgaaattc tttactttct
     1381 tgtctcaaga agaaatcgaa gacttagcga aaaaagtaga gacagaacct gagaaacgtg
     1441 aagcacaacg ccgtttggca gaagaagtta caagatttgt gcatagtgaa gaagacttga
     1501 aagaagcgca aaaaataacg caagcattgt tctctggaaa tattaaagaa ctaaatgcgg
     1561 aagaaatcgc gcaaggattc ggtaaaatgc cgaacgttga aatttcaagc acacctgaaa
     1621 atatcgtgga actgcttgtt tccacaaaaa ttgaaccatc taaacgtcaa gcacgtgaag
     1681 atgtttcaaa cggagctata agtattaacg gtgaccgcgt taccgattta aattttgtca
     1741 taaatccatc cgatgaattc gatggtaagt ttgtggttat tcgaaaaggt aagaaaaatt
     1801 actttttggc caaagtaatt gattagaatt tagtaagttt ttaccttgaa aaagggaaat
     1861 actattacaa cattccaatt ttgaaaccca tcctaaaata gaacgctggt tgtcgttaat
     1921 acaatctttg cgagggagtt tacatttgta aattccctcg ttttttttaa ttatttcaaa
     1981 agtgtgaaca attcggataa ttgatacata ttaaaatatg attaaaaatc aggccaaaac
     2041 ttagtttaca gaatagaaga acaggtgtaa tgtagggctc gacaaaattt aaatattttt
     2101 ttaggagggt ttcactatga gtgaatcatt gtcgaaagat ctaaacctaa atgccctatt
     2161 cattggggac aaagctgaga acggccaaat ctataaagct ttgttgaatg aattggtgga
     2221 tgaacattta ggctggcgtc aaaactacat gcctcaagac atgcctatta ttacgccaga
     2281 agaaaaaagc agtgctagtt ttgaacacac tgtgaataaa acaaaggatg ttctatcaga
     2341 aatttcagcg cggatgcgta cccattccgt tccttggcat aatgccggac gttactgggg
     2401 tcatatgaac tctgaaacat tgatgccatc tttactagcg tacaattttg ctatgctatg
     2461 gaatggtaat aacgttgcct atgaatcttc tcctgcaaca agccaaatgg aagaagaagt
     2521 aggaatggaa tttgcaaaat taatgagcta taaagatggc tggggccaca tcgttgctga
     2581 tggttcttta gcaaacttag aaggactttg gtatgcccgc aacatcaaat cattgccgct
     2641 tgcaatgaaa gaagtgacac ctgaactagt tgctggaaaa agcgattggg aattaatgaa
     2701 cttgtcaaca gaagaaatca tgaatctgtt agacagcgtc ccagaaaaaa tcgacgaaat
     2761 caaagctcac tcagcacgta gcggaaaaca tttagaaaaa ttaggaaaat ggttggttcc
     2821 acaaactaaa cactattcat ggctaaaagc tgccgacatc atcggtatcg gtttagacca
     2881 agtgattcca gtgccagttg accacaacta tcgcatggac atcaacgaac ttgaaaaaat
     2941 cgttcgtggt ctagcagctg aaaaaacacc aatcctaggt gtagtcggtg ttgtaggatc
     3001 aacagaagaa ggagcaatcg acggtatcga taaaatcgtt gcattgcgtc gcgtattaga
     3061 aaaagacggt atttacttct acttacacgt agatgctgct tatggcggat acggccgtgc
     3121 gattttctta gacgaagaca acaacttcat tccatttgaa gatttaaaag atgttcatta
     3181 caaatacaat gtctttacag aaaacaaaga ttatatctta gaagaagtac acagtgcata
     3241 taaagcaatc gaagaagcag aatctgtaac gattgaccca cataaaatgg gctatgttcc
     3301 atattctgcc ggtggtatcg tcatcaaaga tatccgcatg agagacgtta tctcttactt
     3361 tgcgacatat gtatttgaaa aaggcgcaga tatcccagca ttacttgggg cttatatctt
     3421 ggaaggttca aaagcagggg caacagcagc cagcgtttgg gcagcacacc atgtcttacc
     3481 tttgaatgtc acaggatatg gtaaattaat gggtgcatca atcgaaggtg cacatcgttt
     3541 ctacaacttc ttgaatgact tgtcatttaa agtaggagac aaagaaatcg aagttcatcc
     3601 attaacttat ccagatttca atatggtaga ctatgtattc aaagaaaaag gcaacgatga
     3661 cttagttgca atgaataaat tgaaccatga cgtatatgat tattcttcat acgtgaaagg
     3721 aagcatttac ggtaatgagt tcttaacttc acatactgac tttgctatcc cagactatgg
     3781 caacagccca ttacaatttg tgaaccaact aggattctca gatgaagaat ggaaccgtgc
     3841 cggaaaagta actgtattgc gtgccagcgt aatgacacca tacatgaaca aagaagaaca
     3901 ctttgaagaa tatgctgaaa aaatcaaagc agctcttcaa gaaaaattgg aaaaaatcta
     3961 cgcagaccaa ttattggcaa gcgaagcaaa ataagcaagg taatcatgtt agaaaacagc
     4021 ttgaatacta actgtttctg acgcatgttt ggatctgaag agcaagggga gacctaagct
     4081 cttcgatccc tttgttttga ggttgtcttt gaagaaaccc ttcaagcaat agactcaaag
     4141 aatcccctca aatattcgct gaattgtttc gtttattgct gaaactactt caaagacacc
     4201 gttattttat ccaaaaaatc aaggagagtg tcaacggtga gtaaatctga agtaagtaaa
     4261 atgggtcttg ggacctttat cggtctgaca atggcactgt gtgcaacggt tcggagtatt
     4321 cctacgttag ccgcagtcgg ttggacattg attttttatt caatttttgc agttttgttt
     4381 tttgccggtc cgatatcaat ggtttccggt gagctttcca cgatgcttcc tcaagaaggc
     4441 ggtcctcagt tatgggtaaa aacagcactg ggcagcaaat ggggatttgt cgttgcatgg
     4501 ctattatggg tgcaaatgtt cccaggtatg gtaatggtcg cttctacttt aggaccattg
     4561 ttaggaaaca catttggtaa tgtagaactt ggcaataatc actggttcgt tttaggttgt
     4621 attttagtta tctactggat catcacgatc ttgaacttga aattcgatat ggcaaaagtc
     4681 ggcgggaaca tcggtgtatg gctaggggta tatatcccag tagtcatcat gttcgtttta
     4741 ggtgttttgg cagccttcaa agtcggacta gtctcaaacg gctatctagg tgacttttca
     4801 tggtcaaaag ctttccctga cttggaacat attgattcat tgaaatactt agctggtatc
     4861 acatttattt ttgttgggat cgaaatgagt tctgtttaca tgcctcgttt gaaagacgca
     4921 acaaaaaatt atacaaaagg cgtatttatc gctttgatcg gattagtttt attgaatgtc
     4981 atcaatgcga tgcttgtagc aaatgtcgta ccagatggaa aaatggaatt agcaaatatc
     5041 acgcagccta tcttgattga ctgtcaaatc ctaggcttac cagaagtgat cggaaatatc
     5101 ttcagcttca tggtatttat tggtgtgctt ttacaattat cagcttgggt tactggacca
     5161 tcaaaaacaa tcatccaagt agctcgtgaa ggattccttc caccaaaatt cggattccat
     5221 aaagaaaaca aatacggcgt atctagaaat gtcgttctaa cacaatcaat cgttatttca
     5281 ttatttgctt tgctttatgg tgtaatggat gacgtaagtg ctgtattctt gacacttaca
     5341 aatgctacaa ctgttattta ttgtatcgtg tacattttga ttgctgtttc attgcttaaa
     5401 atgcgtaaga aacatccaga attcgaacgt ccttaccgaa ttggtaaaaa cggaaacgga
     5461 ttagcttggg tagtcagctg catgctgatc ttctctatca tcgttgtcgt atttgctaca
     5521 ttaggtacag cgacattgag tgatgcatta ttagtagcag ctatcacagt ggtcatgttc
     5581 gttattccat tgatcatcaa tcacttcaag aaagattctt gggggattga ggtagaaaaa
     5641 agtttagaag aaaaataaaa aaaataagtc tttcagccga cgttttgtcg ctgatttagt
     5701 aggaggtcct gccaagtgaa tagttttttc atggcagggc cttttcattt gaaataggag
     5761 atcgacgatg aataaaaata tttctttaaa agaaagtatc gctgtattga tcgtgttgtt
     5821 ggcactccta ggagtactga tcatcggatt ccagctctcg ccacatatcc cgatccttgc
     5881 agctttcatg tgtttgttgt tttatggcag atttaaaaag ttcacatggg atgagatcca
     5941 tgacgggatc atcaaaggaa tcactccagg gatcattcca atcctgattt ttcttttgat
     6001 tggtgtctta gttgcttcat ggattttatc tggaaccatc cctacgatta tggtttatgg
     6061 atttaaactt gtttctgtgc gtttcttttt accgacagca tttcttgttt gtgcaattgt
     6121 cggagtgact gtcggcagtt catttacaac gatttcgacg attggaattg cttttttggg
     6181 gatcggtcat ttacttggat tcaatgatgc aatgactaca ggcgcagtcg tttccggtgc
     6241 ctttttaggg aataactgct cgcctttatc agatacgacc aacttggcag cgggtattgg
     6301 aggagttaat ctattcgaac atatcgctgg tatgcgttat acggatttcc cagcattcgt
     6361 cctttcgttg attttttata cgatattagg acataacagt cattcagccg atctagctag
     6421 tatcaatgaa atgatccaaa acatgaaagc gagcttttgg atctctcctt ggacattgat
     6481 tccactagtt attttatttg ggggggcaat caaaaaaatt ccggctatcc ctacattact
     6541 agcgggatcg acagctgctc tcgttctttc ttttatccat gagaaaagtt tgacggtcgg
     6601 aaaagcagca gacatcttga tgaatggtta tgtcgcaaat acacctgatc caaagctaga
     6661 tgaactgctt tctcgaggcg ggatcatgag tatgttaggt tctgctagtt tgattttact
     6721 tgcattggca ttaggtgggt tactgatcaa atatctgatc gttgatacca tcgttcaaga
     6781 attaaaagaa aagatggacc gaccttctcg tttagtcggg tttactgcct tgagctgtat
     6841 cggaatcaat ctgatcgttg gagaacagta tctgtccatc attcttccag gagaaacatt
     6901 caagcgttct tttgatgacg ttggcttatc aaaaacctat ttgacacgaa cactagcgga
     6961 ttcaggtgcg accgtcaatt ctctcgtacc gtggggagtc agcggcacat tcattatggg
     7021 aacgatgaaa gttagcgcgt tgcattactt gccattcgtc tttttctcga ttcttgcacc
     7081 tgtctttaca atattaggcg gttttttatt gaatcgtcga cgtgtaaaaa attgaaggaa
     7141 ggaaaaaaga ggctgggaca gaagcgttta actccaagaa ataagaagaa actcaagaaa
     7201 attgcttttt aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg
     7261 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt
     7321 ttttacagat aaacaatgtg ttagaagtag tatcttcttt tacataaaaa gctgaccata
     7381 ggataaatgg tcagcgtatt tagtgagtca gctatttagt atgggttttc taaaggaacg
     7441 actgtctctc ttttgatcaa gcgatgaggg atgaccaaac gtacgccact gctcaattct
     7501 tcatggatca attcgcgcag cttttgcata gcgacacgtc ctaattcgga gatatcgata
     7561 tcgatacttg taaggtaagg gtgtgttaat gttgagaaga tcgagttgtt gaagctaatg
     7621 acggagagtg tctctggaat gacatagccg tacatttgcg ccaactgcat cgtgcgaagg
     7681 gcgaagatat cgtcaatgac caccaatgct gtggcttcgt tttctttcaa cgttttctca
     7741 aaggacagat aatcctccgg atgctctagt actacactgg ataacgcagg aagaccagcc
     7801 atcatcattg ctttttgata accgaaatat cgttcgaaat acaaactttc atgagtcgtg
     7861 tttgtcacaa acaggatatt cttatgtcca ttctggatca aaaattctgt tgcttgttta
     7921 cctagaagct ggttatcatt atctacataa acgatttcgt tctcatgatg ataaggctgt
     7981 ccgatcaaag tgaaagggat gtggttttgg tataaataat caatgacagg atcttgacta
     8041 tctgaataca ctagaataaa accatctact tgtttttgta aatgcatacg ctggacgttc
     8101 tctaacagca attcaaaatt tttagcagta gcgattgctg tagtcatatc ataattccgc
     8161 gcttcttcgt tgattgcttc catgatctca agataaaatg ggttaccgat ccgctctttt
     8221 gaatcaagag ggggtaagat tacgccgatg gcactggaaa tgcgttttcc tagattgcgg
     8281 gcagtcatgt taggaacata acctagttca tccataattt ttcggacttt cttctttgtt
     8341 gtctcggaaa tactaggatg atcgttgatg acacgagaga cggtcgaggt ggcaacaccg
     8401 gcttttttcg ccacatcttt tacggtaata gccatgataa aaacaccttt ctcttgttat
     8461 tgtgcagaga accgataaat cgtctgactg atccgttctt gaccaggatg gaggttgatc
     8521 gatccaaatt tggggaaatg aacgatatca gggatttctt gcggttcgat tgctaatccg
     8581 tagtttgaat gcatctgttt tccattgaca gagaaatcag cttcgaatcc agtcgtagaa
     8641 aataatacca tacttttatt agaagttgct atagtcattt ggcgaccaga atgcttctcg
     8701 aataattgta aggaagtttt ggaaagccta ggatgatgca aaagaaatac atcatctagt
     8761 ccatctggat aacatgcaag gatctcttgc agtgttgggg attttctaaa gtcgattggc
     8821 agtttttctt tttttgtcca atttccagta ggcagttttt cttgatctaa ttcaagcaca
     8881 ccatctaaaa agaccgttaa ctgatgttca tagatgtctc ttgttccatt tcctgaaagg
     8941 ttgaagtagg catggttggt cggattgcag atggtttcaa caaggctact agcagaggtt
     9001 gtcatctcta attcgttttt tgttaaacgg taagtgatcg tagcagtgat tggtcctgga
     9061 taggtagaaa attcatcttt taagtaaaag accacaccta ttgatgatgg agttttgaaa
     9121 acttctactt cccaaaattg gaaagaccag cctttcgtgc caccgtggat atggtgtgat
     9181 ccgcagtttt tctccaatct atgctcaccc caagaagcat cccggatcct tccggctaca
     9241 ggtccgacag ttgctccaaa aaagcttttg tctgttagaa catcttcgaa acgatcgtaa
     9301 gacaataaaa cattttctga ttttccttct ttgtctggtg cgaagatttg gtgcaggcgg
     9361 gcaccgtaat tgagaagaac gacttctaag tagtcgttct tcaatgtgat tttctttatt
     9421 tcagtctctg gaactggtac gacggagatc tctggattat ccatggtcat gacacttttc
     9481 tttcaagtaa tcgatcgtaa gaaaagtcgt atcagggaca agcgggatct cctctccaag
     9541 gtcttcagca cgtcccacgc caattggttg agcgccactt gagaggatgg cttggatacc
     9601 ggcttgcgcg tcttcgatcc cgatacattc ttctgctttt agtccgacag cttttgctgc
     9661 aaggaggaag atatctggcg caggttttcc gtttgttgct tctgccgggt cggcaatagc
     9721 atcaaagtaa ggtgtaagac ccattttttc aagtaggaat ggcccgtttt tacttgcaga
     9781 agcaagagca atcttgattt tttcttcttt taaagaatcc agtaaaggca cgaccccggg
     9841 gaagacatct tttggttcga tcgtctggat catctctaaa taataatcgt tttttctttt
     9901 cgctaattct gcaaattctt cctttgaaaa agagtgttct tttccgccat aagctaaaag
     9961 caaagagagt gagtcttctc ggctgacgcc ttttagctgt tcgttgaatg tgtggtcgat
    10021 cgtaatgccg atttcctgac caagctttcg ccaagcacga taatggaatt cagcagtatc
    10081 ggtaatgaca ccgtctaaat caaacaatac ccctttaaac atttgcttcc tcctttctta
    10141 gcgttatttc tagacgatcc gctaaagaat aagactgttc atagacgtaa atttctagag
    10201 gaaaaccttc taataactcg aaagtaactt gttcatctgt gactgagatg aacaataaac
    10261 gtccgcgata attgatatgg aaagaataag ctttccaagt ttttggcaga aatggtgcga
    10321 atctcaactg atcatgggca gttttcattt gagcaaatcc ttggacaatc gacaaccagc
    10381 taccagtcat ggaagtgatg tgcaagccgt cttctgtgtc gttattgtaa ttatccaaat
    10441 ccaagcgagc tgttcgctgg tacatttcta cagctttgct ttccattcct aattctgctg
    10501 ctaaaatcga atggatactt ggcgaaaggg aagactcatg gacagtcatt ggttcataga
    10561 aatcaaagtt tttttgcttt tcttcttttg taaaagtatc attgaagaaa taaatcccct
    10621 gtagtacatc tgcctgcttg atgaagcaag atctaaggat tttgtcccaa gaccaatgct
    10681 gattcagagg aagatcgctt gtgggaatat cggatacagg catgagatct ttatctaaga
    10741 atgtgtcatg ctggacaaag attcctaatt cttcgtcaac tgggaagtac atgttctctg
    10801 cgatatcttt ccatttggct agttcttctt cgctgatagt gatagcagct tctttttgat
    10861 aagttaaata attttctcgc gtgtaacgca agacccaagc tgcgatcgta tttgtatacc
    10921 aattgttatt gatattgttc tcatattcgt ttgggccggt cacaccgtgg atcatgtatt
    10981 tatttgctcg ttttgaaaaa tggacacggt ctgcccagaa gcgtgcgatt tctgctaaaa
    11041 cttccagtcc ttcatttttc aaatattccg tatcgccggt gtaattcgta taattgtaga
    11101 ttgcataggc aatcgcgcca tttcggtgaa tctcctcgaa tgtgatttcc cactcgttat
    11161 ggcattcgac accagtgaag gtgaccatag gataaagcgc gccttttaat ccttgttgct
    11221 gagcgttgtg gattgcttgc ggcagttgat tatgccgata tttcagcaga ttcttagtta
    11281 cttcaggttt ggctaaagca agataaagag ggacagcata cgcttctgta tcccagtaag
    11341 tggctccgcc gtatttttct cctgtgaatc ctttggggcc gatattcaat cgttcatctt
    11401 cgccgtagta agtagagaat agttggaaca aattgaatcg aataccttgt tgtgcttctt
    11461 cgtctccttc gatgaccaca tcggcaagct tccatcgttc tgcccaagcc gctgtttgct
    11521 cttcttttgc ttggatataa ttgtttgtct ggttcgttaa ttctgagaca gctttttctg
    11581 cttgctgcgc ttctggaata tctctactag taagaacgat aatttctttt tccactgacc
    11641 atgtctcacc ttgagataat tctgcagaca agcaaccatg taatgctaaa gtatcttcgt
    11701 gatagtcagg gatgactgat tttccattaa cgaaatggcg cattgctgca gtaacagtaa
    11761 attgttcaat cccaaagttg tttggaatag ttttagtcgt caaatagccg atttcttcta
    11821 tagttccacg atcaatctca ttccaaaaat gttcatcata gttgctgtct tcgttttgaa
    11881 catttccatc caattttgat acgagtttga ctgttccgct accttcaagc atttctatag
    11941 ttaaatagat ataagctgat tcttttttga caatgctcag aaaacgttcg aaggaaatgc
    12001 gtactttatt agtagaagta gtgattgtga atgtacgaga taatacacca tttttcatat
    12061 ctaattccca gtaaaagtct tctggttcca aagaagccaa gtcgatcagc tgatcgttga
    12121 tcgaaatatc tatcgcgata aagttcaagg cgttgatcac ttttccaaag tattctggat
    12181 aaccattttt ccaccagccg acacgtgtct tatcgggata ccagacaccc gccaaataag
    12241 taccttgatg gtggtcccct gagtaagttt cttcaaagtt cccgcgcatc cccatataac
    12301 cgtttccaat acttgttaaa gattcttgca gtcgaagatt ttttttatct aattgtgtcg
    12361 tgctgatttt ccatggatca atagcaaata gtcgtttgat ctgtttcatt tttctcctcc
    12421 taatgagctg ttttatacgt tcataataaa cgcaaccgat tgcacaagtc aatagacaaa
    12481 agaaaatatt tcatagaaaa aagcttaaaa aaataaattt ttaggagaaa attatagaag
    12541 ggaacacacg tattataggt tttatagaat ggtatagaca gaaaaataaa aaataaagca
    12601 tttacaaaaa agaaaacgca cgctataata cgtttaacga aaccgattgc gtaaaggagc
    12661 taaacggaat gaaaaagata tttagttttg agttttggca aaaatttgga aaggctttaa
    12721 tggttgtggt agcagttatg ccggcagctg ggttgatgat cagtataggg aaatcaattc
    12781 ccttgatcaa tcctgattgg acgccgcttg tcacaacggg cggagtaatt gaaaatattg
    12841 gttgggcaat tattggtaat cttcatattt tatttgcttt agcaatagga ggaagttggg
    12901 caaaagaaag agcaggcgga gcatttgcag ctgggatctc atttatttta atcaatcgga
    12961 tcactggggc tatctttggt gtcacaagtg acatgcttgc gaatgaagat gcatttaccc
    13021 acactctgtt cggtacgaag atcatggtta aaggattttt tacaagtgta ttggaagcac
    13081 cagctttgaa tatgggagta ttcgtaggga tcatcgctgg atttgtcggg gcaatggctt
    13141 ataataagta ctataattat agaaaattgc cggatgcttt gtcatttttc aatggaaagc
    13201 gctttgttcc gtttgttgtt atcttatggt caacaatcgt ggcattgatt cttgctgtgg
    13261 tgtggccaaa cgtacaggct ggaatcaata attttggttt atggattgca gaatcacaag
    13321 aaagcgcacc gattctagca ccgtttttat ttggaacatt agaacgttta ctattgccat
    13381 ttggattgca tcatatgctg acgattccta tcaactatac acagcttggc ggatcgtatg
    13441 agattttatc aggcgcacag gcggggacac aagtatttgg gcaagatccg ttatggttgg
    13501 cttgggccac tgatttagtg aatcttaaag gtgcagggga tatgtcgcaa tatgagtttg
    13561 tgttgcataa ctggacgccg gctcgtttca aagtaggaca aatgattggt tcttcaggta
    13621 tcttgatggg aatggctttt gcgatgtatc gtaatgtcga tcctgataag aaagcaagat
    13681 ataaatcaat gtatttttca gcagcgttgg ctgtctttct aacaggtgta acagaaccat
    13741 tggagttcat gtttatgttt gctgctgtgc cattatatgt catttattca gtgatccaag
    13801 gggctgcatt tgcgatggca gatatcttgc cattgcgggt ccattcattt gggaatatcg
    13861 aattgctgac tcggacaccg ttagctatca aagcgggtct aggtggagat cttatcaact
    13921 tcgtgttgtg tgtgatcgct tttggtgttg ttacgtattt cttagcaaat ttcttgatca
    13981 aaaaattcaa ctttgctaca ccgggacgta acggaaatta cgacaatgac agtgaagaaa
    14041 ccgttgtatc taacagcgga acaggaacag cagatcagca agtggtccag atcattcatt
    14101 tgctgggagg aaaagaaaat atcaaagatg tcgatgcttg tatgactcgg ttacgcgtaa
    14161 gtgtaaatga tcgagaaaaa gtagggactg aagaagcctg gaaacgagca ggggcgatgg
    14221 gattgatcgt caaagacaat ggagtccaag cagtttatgg accaaaagca gatgtcttga
    14281 aatcagatat cgaagatctg ctgcaatcag gagcagagat tccagagcca gaaatggttt
    14341 ctacaggaaa tggacaagca ggtagtaagc aatacttagg tatagaacaa gaactagttt
    14401 ccgcagcttc tggagaagtg attcctttat ctgaagtaaa agatccggta ttctcacaaa
    14461 aaatgatggg tgacggcttt gctgttattc caacatcaag agaagttgtt gcgccgatcg
    14521 ctggaaaagt gacaagtatt ttccctagca aacatgcaat tgggctggaa acgaaagatg
    14581 gtatcgaagt tttgatccat atgggaatcg atacagtcca aatgaatcag ccagcttttg
    14641 agattgctgt caaagaaggc caagaagtag gaacaggaac gaaattagct gagatggatc
    14701 ttgatgtgat ccaaaacgaa gaaaaagatc caacgatcat gatcgtattt acaaacgata
    14761 aagtagaaga agtagtgatc aagcaattag gtacagtgac agctggtaaa gtgatcggct
    14821 ccattaaaat gtaaggagag gttgtgaaaa agctgtcctg catcaagtca taagaacagc
    14881 caaacaaaaa tagctcctcc tatttttcgt ttggttgggc ttattaccga gaagcagcag
    14941 tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag tgataaaaac
    15001 agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt agcaggagaa
    15061 cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt ttcgaaatga
    15121 agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg cttgagccag
    15181 aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat tatgaagtga
    15241 ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct gcgaagctaa
    15301 tgaagttctg tcaggtaaag aatcaagtac ctatccgtaa agataatttt gcctaccgtt
    15361 tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa atgagccaca
    15421 tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca ttggaaagtc
    15481 aggtccttac cgtctcccaa tctcaaaaga aagaagatta tcagacacgc aagatcttga
    15541 ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac ttttcttggt
    15601 ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac tactttttgg
    15661 aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac tctcaaggtt
    15721 attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc gcgaatgaaa
    15781 aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat acagaaaaat
    15841 tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat caacggattt
    15901 ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc gaattagatg
    15961 tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa aactctccaa
    16021 acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt gcctaagcac
    16081 gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag ccaatcaaag
    16141 taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg agctcgttga
    16201 ttcctttttt ctgcttcacg attggggtcc cgatatctgt tacttcataa tcagtcaatg
    16261 tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata tgaacagttt
    16321 tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt tttataaaag
    16381 ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct tttgtctcct
    16441 tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg gtagacatat
    16501 ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa gtatgtcgac
    16561 tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg atcgtctgtg
    16621 ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt tcagaccact
    16681 ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg aaaaatcgaa
    16741 atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc gtccatggaa
    16801 tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt ttttggaata
    16861 aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc aatataggaa
    16921 aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt tgactcattt
    16981 ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct aatcctgccc
    17041 aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga agcagtgctt
    17101 ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac atcaaagcta
    17161 acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca gcgactttga
    17221 taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat aaaggataaa
    17281 aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata gaatacgtac
    17341 tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca tatctcagta
    17401 aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga atctgataat
    17461 gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa aatgggccgt
    17521 aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga aaataatgga
    17581 aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg aatggggcat
    17641 cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc aaagcaatga
    17701 aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg caaactttag
    17761 ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat tcatgaaaat
    17821 atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg atgcgacata
    17881 agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga aacaaagtag
    17941 ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta ataagctcac
    18001 tagcctagaa aatatgagaa gctgttttct gactagcgct cttattactc aaagcataac
    18061 gcagttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    18121 ccaacccaac aaaaaatatg agtagctatt tttttgggct gtacttatta cttgatgcag
    18181 gacagctttt tcacaacctc atcggctttc attttctatt tttttcctga acccatataa
    18241 taaaagaaag aacaaaaaaa cttgcaaggg aggacacatg tgaagcagag taagcaaaaa
    18301 cagtctcgtc gacttttgtt tcgtttgttt ttattaatat gtatcgtcgt agggttactg
    18361 atcgctttat atccatttta tgtggattct ttaaatagtt ttattgatca aaaacgtatc
    18421 gaacaagtac aaaaacggac cgcagctgaa attgaagtgc agcgaaagaa aatggaggag
    18481 aaaaaacaac gattaactga ccaaggattc aatcctggta ctgatccttt caatgaaaag
    18541 aacagaaatg aaagtacaac atccagccaa ttagaggaat ggctgatcgg aagtatcaat
    18601 atacctaaga tccaattaaa tatttcactt tatgatcggc tcaatggtag gatccttgaa
    18661 aatggagcag gcgtcttgca aggaacctct ttcccgcttg gtggaaattc gacgcattca
    18721 gtgatctctg ctcacagcgg tttacctaat cgacggttat ttaccgaatt agatcgttta
    18781 gaaaacggag acacgtttat tttgactgtt ttgggggaga aattggctta tcaagttaaa
    18841 aatatacaag tggtactgcc agatgacacg tctgttctaa cgatttcaga aggagaagac
    18901 ttagttacct tgcttacatg tacgccgtat atgatcaaca cgcatcgttt gctagtgaca
    18961 ggacatcgga ttccttataa cgaatcggta aaaaaagaag aaggaaaagg aaatcaggaa
    19021 agaaccatgc ggcaatggtt tattcttgca ggaacgataa tagctgtagt catactgtta
    19081 gtgtttatcg gacgactaat ctatcagtat agattaagta aaaaagtgct tgatttttca
    19141 tttattatta gtgattcgtc agggaaaccg gtaaatggta gttcattcat tttgaaacat
    19201 aagaaaaaaa cacttactag aaatggtgtg ccgttttcag tacaaagcga ccatcatggg
    19261 aaagtcaagc tggatcaatt gcctggtgga acttatcgaa tcgtttcagc tgatgatcct
    19321 aaagttgctg tttcttttgg tattagaaaa gtaaaacaag agaagatata cttttttgag
    19381 gggcgaaaac ttgtaaaaga gcttcaaaaa aatggttttt attttaaaat caatgaataa
    19441 gtaaggagag aaaaaatgat ccgcacaaca acaaaattgt ctgccaaaga actggaatct
    19501 gttctatcga tttggctgca tgctaacttg gattctcatg aattcattcc atcaacttat
    19561 tgggagaatc aagtagaatc tgttaggaaa gctttaccag atgcagtgat ttacttagca
    19621 gaaatagatc aagaaattat tggttttgct ggggtgattg atcagtttat cgcaggaatc
    19681 tttgtgaaaa gggaataccg gcaacaaaga atcggtacgc aactaatgaa aaaaataaaa
    19741 gaaaccaata aaacactgac tttgacagtc tatcaaaaaa atcaatcagc tattcatttc
    19801 tatctttcac aaggttttac aattcaaaca cagcaaaaag atgaagcagt aaatgaggag
    19861 gaacttgtga tgaaatggaa tcaaaacgct gatttttgat gaacagaata acgaaataat
    19921 gctcccagcc taattttttg ctgggagcat tatttcattt tgaagtcact aatttaactg
    19981 tgtgaatatg cagttgttac aatctttgat aaacggtgtt caatcagctg accctcggta
    20041 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc atttgtttct ctaatactca
    20101 ggtcaaaacg ctgatttcac aacctcttga taaacggtgt tcaaacggct gcttctcggt
    20161 aataagccca ctagcctaga aaatatgaaa agctattttc taactagcgt tcttattact
    20221 caaagcataa cgctgatttc acaacctctt gataaacggt gttcaatcag ctgaccctcg
    20281 gtattagctc aaaaaacggc aaaacatgag gaactatttt tccgtttctt tctctaatac
    20341 tcaggtcaga acgctgattt cacaacctct ctttcacaac cttattttac tcgtttataa
    20401 ttttcatcga agtattttcc gctacggata tcatctggca ttccatcgta tggttcttcg
    20461 ctaataacat cttcattgtc tatacctgca tcacccatat aagtgatcgt agcaaattca
    20521 ggaacaagta attttggata agtttcatat ttttcacgag ctgcttccat ttctgcgata
    20581 tgttcgtttt ggaaagtaac agtgatttct ggatgttctt caacccattt agggaaaaag
    20641 atcattccgt gaagtttctt ttcatttggc ataaagtcca atgccacgtc tgtccctgtt
    20701 ggctctgtcc aagtgatttt gaatacgccg tccgtcaatt taacgatatc agcttcttgg
    20761 tcttttaccc aacggccttt gaccattccg ccatggatac ggtaatcaac agtatggtca
    20821 tttttggcat accattcata ttcccaacca ttgtcatacg tatagataaa gtgagttcca
    20881 ataaaatctt ccaattcttt aaattctttc atgtttaagc atctccttat aaattaaaac
    20941 atgtaataaa ctacatgttt tcgattactc atttatgata gaatggattt aaagatttgt
    21001 caaggaggga aaactgatgg caagaaaaaa tacacttcga tatattttgt tagggttatt
    21061 aagtaagaag aagatgacag gatatgaatt gaatcaatcg ttcaaaaatg aaataggaga
    21121 attttggcaa gcaaaacata gtcagattta tcctgaactt gcaaaaatgg aagagcaagg
    21181 aatcattcag catcaagtag agatcacagg tgaaaaattg gaaaagaaag tttatgaatt
    21241 aacagaagaa gggaaggaac aattggcaga gtggattcat acaccaacca atgaactacc
    21301 ggttaatcga gacgaatttg ttctgaaatt gtatttcgtc aaagacgtga atgatccggc
    21361 attagtagaa attattcagc agcagcgtga ccttcatgaa gaaaaacgac ttcatctatt
    21421 agagcgaaaa aaaacgatct ttcctactga aaaagaaaaa catgaaaatt acggacacta
    21481 cctgatttta gcgcatgcga tcaatcggga aacagaatat gcaaattggc taacagatgt
    21541 attagaagaa agtaaccgaa aaaaataaaa aaatatccga acagtgatgg agaattcctt
    21601 tctatatgaa agaaacgcag atcaccgttc ggatattctt atgttcaaac aggccttttt
    21661 tcaagtattt atgtagcatc tacttattaa aaagcttggt acttaggaga ttagtggcgt
    21721 ttagagttct tttaacaact gttcgtagtc agctttttca tcgacaggaa ggagcttgta
    21781 agttaattcg tctgtctcgt tgtcatgaat gatgtacatc ttagcattct cttctttgaa
    21841 gttaccgatt tgtaaaatcg tatagcgttc gtcgatcaat tcgatatctt tgattgcttt
    21901 tgaaaaaact ttacggacat ggtgcatttt cttttggcgg atttctgcct tttctgcagc
    21961 ttttgacaaa gtaaatccgt catctaaata ttttttgatc agttctactt ttatgactgt
    22021 aggtaaacga tattttctag gacaattcgc atcagcttcg atcgaacgaa tgaatccctt
    22081 ttgttcccaa taccttaatt gacgtggaga cacacctacg atatcactta attcactgat
    22141 gccaacaacc aattgatcat tttccaacaa acgtttaagg gaagccccaa ccatttttgt
    22201 cactcctttt gtcctcttgc ttcccttaat tatatccgta ttaacttatg tgtcaagtag
    22261 gttgacaaat agcgaattta ggactaaagt attctagtag tttcagaaaa aattcagaaa
    22321 gaaggagaaa tatgagtaac aaccaacccg tagatattta cggaaaacca tataatcgga
    22381 cacttttagt tgttgtctta ttaattggta cattttgtac cgttttgaat cagaccttat
    22441 tgacgacagc ttttcctaca ttgatgaaag ctttcgatat ttcagcgtct gatgtgcaat
    22501 ggttaacgac tggtttcttg ctagtcaacg ggatcatgat cccaataaca gcatggctga
    22561 tcaataaatt cagttcaaga aatttatatc tttctgccat gacgatcttt ttgatcggaa
    22621 cgattacttg ctttacagca cctaatttta gtacattact aatcggtcgc ttgatccaag
    22681 cttgcggcgt gggagtatcg atgccattat tgcaaaatat catgctatct attttcccgc
    22741 cagaaaaaag aggatcagca atggggatgt cagggatcgt tattggactt gctcctgctt
    22801 taggccctac attatctggt tatatcattg ataactacca ttggcgtgac ttgtttggta
    22861 tggtactgcc aatcgttgtt cttgtcttgg ttttagcatt cttcctaatg aaaagtgtta
    22921 ttccattgtc aaatcctagc attgatattt tatccgctat tttatcaacg attggattcg
    22981 gtagtttact ttacggattt tctagtgtag gagatgacgg ctggggaagc ttgaaagtaa
    23041 ttggtttctt agttgttggt gtgattttca tcgccttgtt ctcatggcgt caattacatt
    23101 tggaacatcc attcttggaa cttcgtgtat tcaaatcatc tacattcacg attgcagctg
    23161 ttttagctgg tgtaacaaat atggcgatgg taggggcaga aatggtcgta ccattgtata
    23221 ttcaaaatat tcgtggagaa tcagcattcc attctggctt gatgttgttg ccgggtgcat
    23281 tgatcatggg ggctatgatg cctgtgacgg gtgcgatttt cgataaatac ggcgcgaaac
    23341 gtttagccat cacagggatg ttcattttga cagcggcaac tgcaccattt gctttcttga
    23401 caaaaacgac tccagtttta tatatcgtga tcctttatgc gatccgtatg tttgggatct
    23461 ccatggtcat gatgccggta acgacttctg gtatgaatgc attgccaaat aacttgatca
    23521 gtcatgggac agcggtaaat aatacattcc gtcaaatcgc aagctcgatt ggtacagcta
    23581 ttttgatcag tgtgttgacg aatgtgacaa aagcaaatat cccaagcaaa tctgtattgg
    23641 ataatacgcc attagcttat aaagatcagg caatcaatgc cacactgtca ggttaccatg
    23701 ccgcgtttat cgtagcgatc atctttggga ttcttggctt tattattgcc tttttattga
    23761 agaaaaaaga acctgttcta atgaaagcgg gggataatgc atgattattg gagttttgat
    23821 tgtcagcttg cttgcttttg ctttaacgaa cgtgttcgct aaaaaaacat ggcaaacctt
    23881 tttatctttg attttcggac taatcttcgt cgcttcacta agtttgatcg tcgctaattt
    23941 gtccaatcac tttggaatgg aaaaagtaac ggaaacaaaa acagaaaaaa ttgtttccag
    24001 tgctgattcc caaggagcag atatgctttt gtataaagct ttagggaatg gaaaagaaaa
    24061 agtgtatctt taccgaacaa atgaaaaaca agaaaaacca aaagcaactg gtacggataa
    24121 cgaaacaaat aaagtagaaa agacagacgg cgatgctgaa aaagtgacga aaactactta
    24181 ttgggaatac aaaaacgaca tgtataaatt ctggttcaat attgctgata acaatcacga
    24241 atatgacagc cgagtgaata cattcaaaat cccagagaca tgggtagaat taagtacgga
    24301 ccaagcagcc aagttagctg aattagtgaa gaaacagcaa agtacgatgg aaagtgaagc
    24361 aaaagcttat gttcaagacg gtatggtaaa agcaatgact gaaaatccaa aaatgagcaa
    24421 agcagagcaa gaacaacgta cgaaagcctt agctgctgaa ttccaacaac aagcattcgc
    24481 aaaattagtg aaagaagcaa aaggcgaata atattttgaa caccatgtag gaggttgtga
    24541 aaaagctgct ctgcatcaag cagtaagaac aatcagataa aaatagcact ccatattttt
    24601 catctgattg ggcttagtgc cgtagatgca agcttttgaa caccgtttaa ggaagaggtt
    24661 gtgagcctgc cgtttaactc tgagtcacaa ggagcaattt caaaaaacag cttctcatgt
    24721 ttgttgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcgaaaa
    24781 aaggaactgc gacaagactt ttgtcacagt tccttctttt ttttgctatg ctatactaga
    24841 tgtaaaaaat aggagaaata aaatgaggga aatttattct tttgaatcaa aaatctatgc
    24901 ttccgaagtc gacaagggcg gggcatatat tatttttccg tatgatataa gaaaagaatt
    24961 cggtgctgga agagttaaag tccaagcaac ttttgacaat atgccgtatg aaggcagtat
    25021 cgtgaatatg ggcgtgaaaa atccagatgg ttcagtctgc tacatccttg gactacgaaa
    25081 agatattcgt aaaaacatcg gaaaagaaat cggtgatatt gtagcagtta cagtgaagca
    25141 aaaataaaag aggttgtgac aaaagacgtg tcacaacctc ttgataaacg gtgttcaaaa
    25201 gccagtttct cggtagtaag tcaaatttat gcaaaaatgt ggaaacccat gtttacaaaa
    25261 tttttcttac tactcgaaac gaagcggctt tatcacaacc tcttgataaa cggcgttcaa
    25321 tcagctgtcc ctcggtatta gctcaaaaac ggcaaaatat gagaagctat ttttccattt
    25381 ttttctctca tactcaggtc ataacgctga tttcacaacc tcttctttca taatgttttt
    25441 tcaccatctt tataataaac tggttctttg atatggaaat ctattttttc caaccaatct
    25501 aaaatcttgt aacctaactc ttctgacatc ggaaaagagg cgttgatttg tgcattggag
    25561 atatggtcaa catggatacc ggaagtgatc gtacaacttc ccggaacggc agttttgatg
    25621 atcacagcta ttttttcggc taagacatcg tctttatgga accgaccatc gtggctaggg
    25681 aaacgtacag tttgcatctc tgtatcacga gaaaatgtag tgactgttcc gatatgtggt
    25741 gtatctccgc ctgttaaagt gatcaataag tctttaccga ttactttaac agcagcttgg
    25801 atcgtgaaac ctagtttggt tacttcaact atcgtttctt tttccatatt aatcaccctg
    25861 ccaacgtttt gaatgctcat ttacaattcc aaattgatcc aaaagtttca ttgtatggtg
    25921 attaacaaga tcttggatcg tttgaggctg attataaaaa gcaggaattg gtggtatgat
    25981 ttgcacacct agtcgtgcta atttagtcaa gttttccaag tgaatggcat ttaatggcgt
    26041 ttcacgagga acaatgacta actttcgttg ctccttcatt gttacatcag cagcacgtgc
    26101 aatcaggttg tcgcctaaac caacagagat acctgcgact gttttcatac ttgccggtac
    26161 gatgatcatt ccatcggtca aaaatgatcc gctggcgaca gcagcaccta aatctttgtt
    26221 tgaataatgg taatcaaaca ggctttcgaa ttcagttagt gataagtcgg attccagctt
    26281 taagttctgt tttgcccatg gactgatgat tccgtgtgtt tctatcatcg gatattcttt
    26341 tagtttcttc actaggttga tggcataaat ggtgcctgaa gcgccactta caccaatcac
    26401 gatttttttc ttccgttgtg ccaattgttc cattttccgc actccttttt atgatgtcaa
    26461 aacagttgca gatcattcat tatttcaaat atttttccca atcttctact tcttggaatt
    26521 ttgcacgtac aaaatgttct ttcatatcga aaggaactgt accgtcaaag atagtttttg
    26581 cactcatccc acggaaacga atagagccag gattatattc tggtaattct gatgggtcca
    26641 atggatggtt cctcattcct gggatcacga tgatatcttt atctccttgg aaacgagtgt
    26701 tgaaagtcca aactacatcg ttcatgtcga aaatatcaac atcttcatcg actaaaatga
    26761 ctgtcttcaa ttctttgaag gctgataaag caagaatagc tgcttgacgc tgaatacctt
    26821 catccgcttc gttttcttta cggatttgca tgatggtcat caatttacca ccacctgcag
    26881 gaggattgta gacatttact acttttcctg gaatagcttt atcgaccaag gcaagaatac
    26941 ttgcttctgt aggaataccg gccatagata cgtgttcttc actgggacca atcgttgttt
    27001 gcatgattgg attgtctttt cggtgagtaa ctgctttgac tttcactact tgtacagctg
    27061 gattagcatc cccagtatag ccaggaaatt ctggcattgc ttttcctgag ttcgtgttga
    27121 tatcttcttg cattgtttca tttggcatga tttctgcttc aatgatgaat tcagcacgag
    27181 caatacccac ttcatcgact gctataccat caacgatttg aataggttcg ttacgcaaag
    27241 ctcctgcaca ccatagttca ttgtagccaa gtggagtggt aggtggttcg aaagtcgtgc
    27301 caatggcaat tgctggatct aaaccaatat tgatagtgat aggcattggt ttattcaagg
    27361 cttcgaattg tttttggaag tgaccaatgt tgcgtccgcc aggcatgatg tacataccga
    27421 tcgtatcttt atcttctaga accatacgat ggatcgttac atcgctcatt gttttttctg
    27481 gatctgatcc taagactaca cccattgtga tgtatggacc agcatcttca ggagtatttg
    27541 taggtgcagg caaaatgttg cgaatatcga agtctgcatc ggtagctaga tgaacgactt
    27601 cttgtgctgg agcgtctgct ttgtctactt tcacaggttt gattggattt tctacagcgt
    27661 ctttcaagaa acgaccaagt gttcgataat catggtgcaa aatttcacct acacgtttac
    27721 gactagccat tgttccgatg ttcacacgtg tgccggggaa acctttgata ttgttgaaag
    27781 tcatagctgg tccttcttga gtcggacgtt ttactgtacc gccagcaccg atataacggt
    27841 aaacaccaga taattctgct tcaggatcga tttcaatatc tgtttcgtga tattgacctg
    27901 gaagtgtttt tagttcttcg atgacttttc ttaaatcata cggttgatct gtcatgagag
    27961 attcctcctt agttgtcttt acctatctat agtaaaaggt gaaaaagagg aaaacaattc
    28021 aacttgttcg gtaaagtatt ctacaatgga atggatgcgt ttttttgata atagaataaa
    28081 ctatgatata cttatcacta aaagaaagcg catacttagt gttggaggaa ggaaagtgaa
    28141 aaaaatgaag gagtcatttc ctaaagcagt aaaagtggaa aatatagcga atattttgaa
    28201 agttacattt gaaaacggag aagtaaaata cgtgaaatct cattggacag aagagataac
    28261 cgatgccttg caatttggaa aaaaaggaag aggaaaaaga aaaaatcttc tcgcactttc
    28321 tcgtaatatg tggattggga ctgaagtaac gattgaagca gatggaacag tattcataaa
    28381 tggaaaagac aggtatacgc cagaagagtt gtggtacaaa ggaaagaaaa gtataccaga
    28441 attataaaaa ataattaaaa aaaacagcgc gaaacaagga acatattact tcctcgtttc
    28501 ttgctgtttt ctactatttc agcgctctta tgactcaaat ctaaatgtgg ttgtcacaac
    28561 cttattctgc acgcaaagcg atagcggcgt cttttttcgc tgccattcgt gcaggaagat
    28621 gaccgccaag catcgtcaat atagtagaga cgatcactag aataatcgca tggacaggat
    28681 ttagttgagc aacatttttt agatccgtca tgttgtataa gatgctattg atagggaacg
    28741 tagctagcca tgcaataaac acacctaata tcccagaaga aatacctaaa atacacgttt
    28801 ctgcgtcaaa tacgcgagtg atgtcttttt tacgtgcacc taaagctttt agtacaccga
    28861 tttcttttgt ccgttcaatc acagatgtat aagtaatgat actgatcatg atcatacttg
    28921 ttactaaaga gatccctgca aaagcaataa ggacataagt gatagcatcc atcaatccgc
    28981 cagtcagttc agtcatcgtt ccagctaaat cagtataaat gattttatct tcatctgatt
    29041 tgcctttatt gtaatcatct agataatcca agatcttttc tttgtcttca aaattgttcg
    29101 gataaatcat gatacttgaa ggcaagctgt ctccacctaa ataactaagc aatgtttgtt
    29161 tggcattttc gtcaactttt tcagtagtta agacatttac accactgtct ctttgagctt
    29221 tgacgatttc tgattctttg ttttcgttga cgatttgagt cgttaattga tcgctataag
    29281 cgattcctgg agataagaga ttcattgtag aagaagattt cacgcgtagg atccccgaga
    29341 tcttcaattc atcagaagca ttctgataca tcgcatcata atctgtatta gggataaaat
    29401 tcccagttgg aagttttgta tagtaagtat tgttgtttac taatttgaat gttgtaccaa
    29461 cgatatcatc aaaatcaagt ttttcgtctt ctttcacatc aaatccaaga ttcttcaatg
    29521 cgttgatatt agtattatta ttgccgtcta cgatcagcac gacatctgtt gctgaggctg
    29581 gataacttcc ggcaagtaaa gaataattgt cttttaaaaa gttttctttg ctagtgtcaa
    29641 gctgtgtagg gaaacttgac actcccacac ctgtcattgc tgacatcgta ctagataaag
    29701 aaagagattc cgtatcagga ttttgattcg agaagctgac aggctgtact ttgccattta
    29761 catcacgtaa aagattgatt cctgtagtac gggtaaatcc gatattatta cttaagttag
    29821 gatcgatgtc tgttacgtaa tcgacgtatg tttgatcaat cttattcgtg tgctgtgctt
    29881 tatcttcttc gctgactttt gcagtaatcg ttttgctgtc tggataatcc gctttgcttg
    29941 cacccaatcc ggcatcgtca cgagtctggt tagtcgttac cttagaaata gtaatcggaa
    30001 atttcgccat cgtttcagct tgcgtgttat cgatttgttt ttggaatcca gaagaaaggg
    30061 acagaacgat cgcgattccg atgatcccaa tactggaagc aaaggaggtc aagaaagtac
    30121 gtcctttttt tgttcggata ttgttgaagg aaagctttaa cgctgtccaa aaactcatct
    30181 ttgttctgcg taagttaaat tgatcatctt ttggtcgttc gatgtgagga tgggaatctg
    30241 ttaagatctt accatcagaa aattcaatga tccgatctgc gtattgatgg gccagttcag
    30301 gattatgtgt gaccatgatc actaattttt ctttcgataa ctcttggatc aattccatga
    30361 tctggatact tgtttctgta tctaacgctc cggttggttc atcgcacaaa aggatatctg
    30421 ggtcattggc tagtgctcga gcgatggcca ctcgctgcat ttgtccacca gataactgat
    30481 ttggcttttt atgcatatga tctgtcaagc cgactcggtg caatgcatct tctgcccgtt
    30541 ttcgtttttc gtctttagaa acaccactta atgtcatccc aagttcaacg ttttcaataa
    30601 tgcctagatg accaatcaag ttgtaacttt gaaaaatgaa accgatagag ttgtttcggt
    30661 aggcatccca gtcactgtcc ttaaagtctt tagtagactt tccgttgatt accatatcac
    30721 cggaatcata attatctagt ccaccaatca cattcagcat agtcgtttta ccagaaccac
    30781 taggacctaa aatcgctacg aattcttttt gtctgaaagc gacagacaca ccatccaatg
    30841 ctttggtggt ggtttcgccg actttataat atttttttat atcttttaat tgaagcataa
    30901 tcacccttct cccgtattga aatattcggt atactaataa gtatatcaga acaatgatcg
    30961 tagtatacga aataaatgtt aactgaagat gaactacaag aaaaaatcta gaaatttttc
    31021 aaaaaacaga gggagatcaa tgaatcgtat tttagtagta gaagatgatc cgcaattaca
    31081 gttgctttac cgttctgtgt tggaacgagc gggcttttca gtcatcaccg cattgaatgg
    31141 agaggatgcg ctgaaaaaac tagaagcgac acgtatagaa ttaatcatta cagatatcat
    31201 gatgccgaaa atggatggct atgacttgct agactcatta cgtagcacca gatttgacac
    31261 acctgttcta atgattacgg caaaggccga ttttgaggat aagaaacgcg ggttcaaatt
    31321 aggagcagat gattatatga caaaacccat cgatgtcaat gagatgatcc tacgtgtgga
    31381 agcattgctt cgacgttcaa agatcaatta cagcagtagc ttgataatcg gacaaacaaa
    31441 attagagcta gatgcttata cagtgttaca agatcagcat tcgttgattc tgcctcaaaa
    31501 agaatttctg cttctgtata aattactgtc ttatcccaac aagatcttca ctcgccaaca
    31561 gttgatggat gatatttggg gattagatac gaatacagaa gaacgaacga tcgatgtaca
    31621 tatcaaacgt ttaaggaccc gttttgaaaa gacggaagat tttcaaatca tcacagtacg
    31681 gggactaggc tataaggcgg ttatttaaag atgaaaaaaa agctattttc acaattatgg
    31741 atctattttg ctagtattgt cttcatttcg atcttagtga cactgctgtg ttttctcggt
    31801 tttattttct tattgtctcg acattctatc tcgcctgctg aatcggctgg aaaaccaacg
    31861 ttatacccgt tattcgtctt tgcggggttc agtatggtcg tgggtacagg aatatcgatt
    31921 tttgttgggc ggcgtatcct gcatccaatc agtgcgctag ggacaaatat gagtctggta
    31981 gcaacgggag atttttcgat tcgaatggat gaacagcaga aagtagcaga agtacagcaa
    32041 ctgtataaag atttcaacgt tatggtccaa gaactcaata gtatcgagac actgaggaat
    32101 gactttgtgt ccagtgtatc ccatgaattc aaaacaccac tagcaacaat ccaaggatat
    32161 gttcaattgt tacaagcccc aaatctctct gacgaagaac gccaaatatt tttatatcgg
    32221 attatcgaaa gtatcacgca gctttcacaa ttaacagaga acatcttaaa attgaacaaa
    32281 ctggaaaacc agcgtatcca actggaaaag aaagagtatc gattagatga acaaatcaga
    32341 gaagtaatcg tttttttgca gcctaaatgg gagaaagaac agctagaatt ggatatcgaa
    32401 cttgctgcag taaattacac aggcaatgaa gagtttttgt accaagtgtg gctgaatatc
    32461 atggataacg ccataaagta caatcaaatc aatggacaga ttcatatcaa actatttgag
    32521 acagccacag agattggact ggaagtgacc gattcaggtg taggaatgaa tgaagagacc
    32581 agagatcgaa tgtttgaaaa attttatcaa ggagatacaa gtcgtcaaat ttctgggaat
    32641 gggctaggac tttcactggt taaaaaaatt ctagaactgc atgatggacg tattgattat
    32701 agcagtatcg aaggagtagg aacgacagca atgatacggt taagtaaaca ataggaaaaa
    32761 gaataaaacc cctaaaaatt ctattgaagg tttttagggg tttttagtcg tttttttgtg
    32821 attagggaaa tgttcttacg gttcttttgg cataaaaatc gctgctttct tattgaacta
    32881 cgagaaatgt tagaggtgtc attgaaacaa ggacatgatg caagaaagag agcgaaagtc
    32941 atgcgtcgct ctcttttttt gggtggatag atggacagaa aatgtagggc ttagttttct
    33001 tcttgattta aatactccca aaatgtatct gaagccaaag aatgctcacc gactttacga
    33061 gtaaatgcaa gataactctc tgttgttgga gaaataggga gaatcacagt attttctttt
    33121 aatagattct tgactgtttt ttccataaca atagcaatcc ccatgtcatt tgcaatcata
    33181 ttgatgatca agtcgattct tgctccttta taagagattt ttggttcaaa tccagcttcg
    33241 cgacacagat ctagtacagg ttgatacaag ttcgtagtgg ggccaaggag caaaaagttc
    33301 tcctttttca gttcgcttag atccaatttc tttttactag ctaaaggatg atttttaggt
    33361 agaactgcta caaaatcgtc tgtttcagta ggcaaccact caatcgaact tggtggggcc
    33421 tggaaatttc tagcaaagtg gatcgtgtca ctatgttcat tggcgtcatt gtaagttaat
    33481 tcgacacttt ccacttcttt caactggact gtaaattcag gatggcgttt caaaaactcg
    33541 gtgattttgg aaaaaccttt ataattgatc attgtcggta tcgttaagat atttaatgaa
    33601 agattgttgt ctttcgtata ggtttcaagc acttcttgca tagcatggta ttgttctacg
    33661 acatttttta catatgggag caataagctt ccagcttcgg tcaattcgat ttttctatgg
    33721 gcgcgttcga ataatttgac gtctaattct gtttctaacg aaagaatctg tttagaaata
    33781 tttccttggg tggtgaattg tcgatcagca gttttggtga aattcattgt ttcggctaaa
    33841 tcaagaaaaa cttcgaattt atggatatcc atcatattcc tcctacttat tccaataaag
    33901 aatactttaa cacaaaaaat gaattggtgt aatcttctct gtctgttaca atctttcttg
    33961 cagcgaaagc aataattaca gaaatttttc tgattttttt cgcttcgtag aaaaagagag
    34021 gagttttttg atgccaactg gagttttgat caatgtaggt tccgttttgc ttggcggact
    34081 aattggcgga ttagtaggaa ataaattaag tgaacatttt aaagcacagc tgacgatggt
    34141 ttttggtgtg tgttcgatgg ggatgggaat ctattccatt gcgccgatga aaaatatgcc
    34201 ggctgttatt tttgctttag tgataggaac agcgattggt ctgattgttc atttaggtaa
    34261 tgggataaat aagggggctg cgctgatgca agtacctatt tctaaaatct tccctagtga
    34321 aaagcttggt atgacccacg atgaatttat cagcacatta gtgacggtca ttgttctatt
    34381 ttgtgcaagt ggtacgggga tctatggaag tttagattct gggatgacag gagacagtac
    34441 gattttgatt tctaaatctg tccttgattt tttcacagca gctatttttg cgtgtaattt
    34501 agggtatgtc gtttctgtga tagcaattcc ccaattcatt attttttata ttttgttctt
    34561 attggcaaaa tttatttatc cattgactac accagacatg atcttagatt ttaaagcttg
    34621 cggcggattc ttgatggtcg ctaccggttt tagaatgatc aacgtgaaaa tgttcccagt
    34681 agctgacatg atacctgcaa tgatcgttat catgccactg agctggatgt ggacaaattg
    34741 gattatgcca ctgttataat aaaaatcaaa gagaagccat tttttgctga ctaaaaagga
    34801 tcaaatcttt tagtcagttt ttttgtattt gacaaactga ttttgaaact atattatatg
    34861 ttgtataata tagattgaaa taaaatatta cacttgagaa tagaaggtga cgtaatgcac
    34921 atccagattc caactgttct tcttgatggt actgtgctag cagcattaag caaggaagat
    34981 atgtatgggt atgccttaac aaaaatcgta caagaaagtc tctctgtcag tgaatctaca
    35041 ctatatccgg ttttgagaag attgaaaaaa gaaggatatc ttgagaccta tgatgaacct
    35101 tttgaaggca gaaaccgtcg atattactcg ttgacaccaa tgggaagtcg tcatttagaa
    35161 gaaattattg atgaatggat tgtttttcga gattctttag accggttatt ggaggagagc
    35221 acaaatgaat gaatatttga atgaagttgt ggatcatctt caagaaattc ctgaagacga
    35281 tcgttttgat ttgatccagt attatgagga atatttttta gattctggaa aaacattaga
    35341 tcagatcatt gatgaatatg gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta
    35401 cttttcagaa gaagaacaag cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg
    35461 cctgatttgg ttaatcatta tcggtttatt tgcttctcct gtcctcattc cactagcact
    35521 ggggtttata ttggttattg ggggactatt ggttgcttta ctagcgctca tttttagtat
    35581 ctatgtttta tgtatcagta tcttaggtgt tggcctattc atgatcatct caggaatcag
    35641 tgtgtttgga caatcgattc tgagtggatt attctttatc ggtgtcggtc tgttcgccac
    35701 aggaagcgca gtatttattt cgccactgtt gctgaaagga acaaaagggc ttttttatgt
    35761 catgatgaac tttgttaaat ggatgggacg acgttttatt acaaaaagag atctgcattc
    35821 agcgggcgtt tgaaggagga cacataaatt gagattgaaa cattttttga ttaccggtat
    35881 tggtctgatg cttactggtg gcctcctagc aggtgcaact tatacattag gcgctcaaaa
    35941 gagtctgact tgggataatg gtcctcggtt gatcgatatg gttactgaga caaaaaaaat
    36001 caaggatgca aacaaaatcg tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg
    36061 attggatttt gagataaaaa cgacttatga taagacagaa aagccagaaa taaaggagaa
    36121 aaatggcgtg ttgactgtga atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga
    36181 agacacaagt gcacaaatca ctattgttgt ccctgcagat catgaattat ctgaattgac
    36241 aatcgaaggg aataatagtg caattgatat caacgaagta tcaatcaaaa aagtgaatac
    36301 taagacacat gatggctggg tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa
    36361 agatgaatta ggctcacttc gattagatga ggccaatgtg gataacttga tcgtcacagg
    36421 tgaaaacagc aacttccatc tagaagacag tttgatcagc aaagaaggaa aagtcatcct
    36481 aaacggtggc aatttatctt tgcatgagat ggaaaacagt ggctatacta tccaaacgtc
    36541 tggtgaatct tatattgaaa aagactacca gccaataaca aatactttgt tagaacaagg
    36601 acagaaaaag ctaaagatca ctgctaaaga cacatcgatc aatattacga cggatcaaga
    36661 ggacaatgaa acagattgat cctgtactca actggatatt ttaagaaaaa acagcaaaga
    36721 tccttccaat ctttgctgtt tttcatcgct taacgcacat aactatagta aaataagaaa
    36781 gaaaatatct aaaaagaaaa cagggtgaaa gagtatatga aggtagaaga aggtcaaaga
    36841 gaaaagttaa aaacagaaat ggaaagatta catacttata tcactcaact aagccaaacc
    36901 ttctatgatc cagacaaaga aaaagtgatg gttaattacc cgaacaattc agaaggtcgc
    36961 caattagagc aagtgtacca tgaagttttc aaacatttat tgacggtgaa gaaagagctg
    37021 gattattatt ctttaccgat tatcgataca ggaatattga agtatgatgg gaagaaggaa
    37081 cgatttatct ttaaatctgt tcgggaagat ttgcctcttt cagcagggat ggatctagag
    37141 gtcttggtag aagactattt tacagaagaa aagcactggg ttcgaacaaa attggattat
    37201 ttaccgcagg cagccggagg gacacaggca aggggttggt acatcactga agacaaagag
    37261 ttagaactag aaggtgtaat ggcgcgtatt cgaaaaaaaa ttaaaaaagt aacattagat
    37321 atttattgaa ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat
    37381 caatggttat gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt
    37441 ctatttaggt atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt
    37501 gatcacgtta ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat
    37561 tttgtttttt ggacaatatt taggtacgat tcgagaaaat ggctttttcc ttacgatacc
    37621 tttggcacaa aaaatgactg tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa
    37681 agtcaatgat ttggatggaa accccatcga aatctcagca gtagtagtct ttaaagtcgt
    37741 tgatacagca aaggcattat tcgatgtagc gtattatcaa gattttgtag aaatccaaag
    37801 tgagacagcc attcggcata tcgctagcca atacccgtat gatacattca atgagaatga
    37861 tttgacgctt cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga
    37921 gcgtttagca gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc
    37981 aacagaaatc gctagtgcca tgctccaacg tcaacaagca aaagccattt tgtctgctcg
    38041 acaaaccatt gttgaaggag ctgtcacaat cactcaaatg gccttagagc aaatcgaaga
    38101 tggacaagaa atcaatttca ccgatgatcg gaaagtccaa ttgatcaata atttacttgt
    38161 ctcaatcatt acagacaaag gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg
    38221 taagtgacat actttttgta agataggaag aaggagttta ggacggatta ttgtcttgaa
    38281 ctcttttttt agttgtttta aagaaaatga caaaattgaa agaagaatga tagttgattc
    38341 gaaggcagaa ttgttcgctt ttttctataa tcagtatata aagtaagaga gaaggggaaa
    38401 aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt tgtggtactt gcaggggtga
    38461 aaatcgcaga ttacgtagtt atgggtggag aagaatacta tgtacaaatt acgacagatg
    38521 gtacaaaaac agtttcaaaa tcagactctg gacaagaaac agtcaactat agatatatcc
    38581 tgcctggcta tacgaaagag ggtaaggaaa aagaactgga cttcaaggga caattgactc
    38641 gaccgcttcg taaaggagca tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt
    38701 cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag
    38761 ggtagggaaa gaaatgaaca aaatagtcga agtacagaat gtaacaaaag tatacggaaa
    38821 aaacaatgag aaaaagacgc aagcattaag cggcatcagc ttcgatgtag aaaaaggcga
    38881 atttattggg atcatgggcg cttctggttc tggtaagtcg acacttttga atattttatc
    38941 cacattagat aaaccaacag atgggcatat ccgaatcaat caaaaagatg tgaccacatt
    39001 aaaaggaaat caactagcag attttcgtgc gaatgagatt ggctttattt tccaagattt
    39061 caatttatta gaaagcttga cagcacaaga aaatatcgct gttcctcttt ctttacaagg
    39121 cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc
    39181 tcatattttg gaaagctatc cttctgagat ttccggtgga caaaaacaac gggtcgccgc
    39241 cgcacgtgca ttgatcacac aaccaacgat tctattaggg gatgagccta caggtgcgct
    39301 ggattccaaa agtgcccggg atcttttaga tacgatggac gaattgaata caaaagatca
    39361 tgtatcgatt cttcttgtga cgcatgatcc gttttctgca agttattgcc aacgtatttt
    39421 attcatcaag gatggcggta tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc
    39481 cttctatcga gaaatactat ctattcttgg aaacttggaa cagtaggggg agattgacta
    39541 tgctttggaa attatcactt actgggatca agagccgttt aagagactac ttggtgttat
    39601 tctcaggatt agttatggca tctgctattt tttacatgtt tgaatcaatg gcaagcaacg
    39661 aagctttttt gaaaagtaat acgatcgttg cggctacagt tatcattttc cgattcggtt
    39721 ctgttttact agggatcatc acattcgttt atattttata tgcaaattcc tttttgatga
    39781 cgatgcggca aaaagactat gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg
    39841 cacagatgat tttttctgaa acattcttag tagggatcgc agctacttta gtgggttcag
    39901 ctgtcggtgt aggattgaca agtatcgtcc accgattatt agtggatcag ttagatctca
    39961 atatttcgca ttttacacct tttagtattc acgggttgtt gatcactgtt ttgttttttg
    40021 ctgttttgtt tttattagct gctgttgtca atgctttttc gattgtcaaa aaaccgattt
    40081 tagcattgat tcgtgcggat cagacaccaa caagaatcaa gcagcataaa ttcttgtttt
    40141 tccttgaagt agtactggga attgcttttc taggctgcgg ttactatatg atgaaacacg
    40201 tggggaccta tcaagtcttt ggtatagggg tagccttagt tacgatcata ttaggaactt
    40261 atttcatttt ccattctgtg atcattttct ttttatcatt actaaagaaa tcagaacaga
    40321 tttcattgaa aaaattgaac aatttcacat tgtcacagtt aaactttcgt attcgtgaat
    40381 atacacaaat gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg
    40441 taggattagg ttttaaaaat gaaattgcag acttgacaga aaacaattct ggatatgatc
    40501 tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat caaagaactt tcaccaacac
    40561 tttctgcttc ttatactcaa aaagaagatg cgggaacgat ttactataat gcaagtgaat
    40621 ttgcggctca accattgatc atgataaaac atgaagatgg aacaccgccg aagatcacga
    40681 aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt acaagaggaa cttcgaagct
    40741 atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc
    40801 aactgaactt gccacaatcc actttacagc tagttcaggt aaaagacttc caagcaaacc
    40861 tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag
    40921 tagagtacag caaccagaaa tatgaaatgt atgaagtgta taatggtcta ttctctggtt
    40981 ttgaatttat gggattcttc ctaggtttag ctttcttgac catgcttgct agctgtttga
    41041 tgttcaagat cttgtcagga agcaagagtg acattgctcg ttatgaaatg cttgaaaaga
    41101 taggaacacg acgcgggttg cttaaacaat cgatccgtag agaagtaggc gtgttattct
    41161 tagcaccagg aatcttaggt gtagtccatg tcttattcgg attacaaatg tttggtctat
    41221 tcatgcaaaa tccatacaaa gatatttggg ttccgtttac gatcttcttc gtattgtatt
    41281 tcatctacta tgtactgacg acatggttat atactggaat cgtattgaaa aagagattgt
    41341 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagct cctcatattt
    41401 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaaaa
    41461 agaggttgtg acaatatttt tgtcacaacc tttttttcac ggtcttttat tagaatatag
    41521 taaatttttt ctcgttactc acggaataag ctgttaagaa aacggtttcc atgtatactg
    41581 gaatcagtgc aaatgaaaga aaagagggtg caaggaacat ggaaatcaat gaacaaaatt
    41641 ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa tgggacatct ttgtctgtca
    41701 gtgatcttgg ggcacgggtt gttcattttc aaacaataat cgataatgaa aaacgagagt
    41761 tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa agacccttat atcggtgctt
    41821 caatcggacg tatggctgga cggattacga atggcacatt caaattagat gatgaaacgt
    41881 tcgaagtaaa aacagacccg gacactggtc attgtttaca tggtggtgca cctagctttg
    41941 aagccaagca gtggcattat acagtcatca atggtgaaaa cgaagcatcg gtcattttct
    42001 atcagactag cccagatatg ggaaacggat ttcctggaac tttagatgta gaagtacggt
    42061 atacgctgac gaacgatgat acatggcgag tgacggtcca agggatcagt gaccgcaaaa
    42121 cagtattcaa tcctacaaat catgtctatt tcaatttaac cggtgatcca gcacagtcaa
    42181 tcgacgaaca tatactttgg ttgaatagcc gattatttgc tacgctcaat aaagatacaa
    42241 cgcctacagg agaagtagtt tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa
    42301 aattaggtga ggtctttgcc agtgattttc gccaatcaat gatagtgggc ggcatcgatc
    42361 atccattttt gttgaaaaaa caatctggtt tactaaatgc tgcggaattg acaagcccag
    42421 atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa
    42481 attttggaga agatggaccg gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca
    42541 catttgagac tcaagaactg ccaggagcgg agcgacttga aagttttgga gatgtcactc
    42601 tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa aataaatata aagaaataga
    42661 ataaattaaa aagggagtgt gacagtgtca catccccttt ttaatttatt ctatttcttt
    42721 aaaggcagat caagccagaa tgatttgtct aaacttaaca aggttagtac aggaacagtt
    42781 gcagctttaa ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa attgttccac
    42841 aaatcccaga ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt
    42901 gaaaattcta atcctgtgat tggcacgcca tctgttgtcc atttgattgg cacatcgaat
    42961 cgcttagaca tattgaggat ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc
    43021 ggttcatcat attttgtcag gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat
    43081 tggcgatctg tagcataacg tccttccaaa tatttggttg cttctttgta gtcttttgtt
    43141 tccgacttcc aagttggttt gtagaaatca ggattgccag tcagtccatt tttgatcaag
    43201 tcggcatagt cttccaaact ttccttgtaa gaaggatatt tacggaattg tgcgcggatt
    43261 tggtagcttt gtccggaact atcttgttca agtgtattga aggtcacgct gcttccttta
    43321 tatgatcctt tgataccaaa aaggttatga tttggttcgc ttgataataa gctgtttcca
    43381 gatccacttt caaggatagc ttgagccatc atgactgatg cgtagattcc ttctttttgc
    43441 ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt ttggttttga
    43501 ctgaaatgga acgagccatc taatccatct aaaatagatg agccgcctgt cagcgaacct
    43561 gaagcagcag gtaaacctgc taagtttact cggacagcaa aatcaggcgt gaaatctgaa
    43621 atagaagtta ctcgaacttt atctcctggt tgaggcgcat ggataaattg tccgttccca
    43681 atatagatac ctacatggtg agtaacaccg gaagaaggtc cccagaagac gagatcgcca
    43741 ggttgggcct gacttactgg aatctgcgta cctgcatatt gctgttcgcc tgtccagcca
    43801 ccgatataat gaccggttgc tttcatatat acgtaataag tcaaaccaga acagtcaaaa
    43861 gtatttggac cttttgctcc ccatacatat ggttttccaa gatgcttgaa tgcttctcga
    43921 acgattgctt gttggacatt cgttgtattt gtaggcaata cagcatcaat tggtgatttt
    43981 acaacaaaat cagaagaagc agaagatgga ttctctatac cgctttgatt tgctgtattt
    44041 ggacgagatg aagcattgtt cgttccgcca ttcgatgaac cattgtttgg atggtttggt
    44101 tctctctgag tatttccatt gttattcgat ccagatgggt ttgtttgatt cgaagaattc
    44161 ccgctgttcg aattattgtt attattgttt gaattatttg aaccattatt attgttgtta
    44221 ttacccacat ctggtttgtt tggcgtttcc ggatctggtg tactagggac ttcttcacta
    44281 ggatcagttg gtttctctgt tgaagtatcc ggtccagctt taaattctgc agtaatagat
    44341 aaattgttcg tacctactgt aaaggtcttt gcatcataat ttacttcttt ggcaccatta
    44401 acagcgactg aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt
    44461 tccgctaata aaccttctac cgttccactt tccgtaatat ccaaaggttg ctgttcaggg
    44521 gaatccgctg tcgaagcagt cagttggatc ttaccaatca aagaaggagc aattgtaagg
    44581 gaatatgtct cggctggctt ttcattttct gatggatcag ccggatcaat ttcagttggc
    44641 tcggtcgatc ctgttgttga gttagttgtt ccagttgggt cttctgtaaa agaatccgtt
    44701 gtttcactag tagctgagct ggatgtagtc gtatctgttg tgactgtttg atcagctatt
    44761 tgattatcta cggcaaatac ggtattggtc gacaaaacag acggagcaat cagccaagta
    44821 gcagctagca aacgtatcgt ttttttattt ttcataataa actagtcctt ttcctttcat
    44881 tgattttctg tcttattcag cattcattag acattttttg gaacagataa tcgtgtcgaa
    44941 aagctttttc ttttcataaa tctgtctgtg tattctaaat aaaacaattt gtaaataagc
    45001 aacgagcatt cattcgtttt tctttgacgt tcattttatt gcatagacca cctttgtttg
    45061 gggataaaca agtggggata aaaatggtta tgcatatgta aattatacca aagtttggaa
    45121 agtatcacaa aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat
    45181 aaacatctca tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa
    45241 acccgctaac gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt
    45301 accagaaaaa agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag
    45361 cgatcgcttt cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag
    45421 cagcaacaaa tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc
    45481 ccgcaagtaa aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac
    45541 ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg
    45601 gaacgattcg gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc
    45661 attggatacc aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg
    45721 gtccgttaat atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac
    45781 ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta
    45841 caaatactaa ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat
    45901 aaaagaaaat acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag
    45961 gaatccccag aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg
    46021 gttcaatcag tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg
    46081 cagcagaaaa agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca
    46141 aaatatgata agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca
    46201 gaccttttaa tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata
    46261 tcaaaccaaa tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt
    46321 gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc
    46381 tatcgagtaa aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg
    46441 ctactggctg ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa
    46501 tattgtcttt tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct
    46561 gttcaggatt cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc
    46621 gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat
    46681 ttcttcatgt gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa
    46741 tatttccggg ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc
    46801 ttctaaaacg ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc
    46861 tattttttca gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa
    46921 gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat
    46981 gtttgtttct tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta
    47041 agggatagaa ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata
    47101 aaggatcgct tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat
    47161 aaatgtatca cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc
    47221 gtgttctttt tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc
    47281 aatacctaat tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct
    47341 gattcctgta atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag
    47401 tagttcagta ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc
    47461 gtcttttgca atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat
    47521 aataaagtca ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc
    47581 attatttgcc cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat
    47641 tgcttgtccg gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt
    47701 tgtcagtaga tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga
    47761 tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc
    47821 gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag
    47881 ggtagtgtcc atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa
    47941 ctatctcatt tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa
    48001 tagttcgata aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt
    48061 ttttctaatc gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga
    48121 tctaacaata acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc
    48181 agtttcggct tgatatactc gcacatatct atattatcat agccaataat gctgtagtct
    48241 tcagggatgc tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca
    48301 tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca
    48361 ccttgttttg agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca
    48421 tcaagagcag atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga
    48481 tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca
    48541 ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa
    48601 acaatgaagg gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca
    48661 gaagtcgcga taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt
    48721 tctttttgat gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag
    48781 atatcctcaa ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt
    48841 acaccaattg ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag
    48901 cctaattctt ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta
    48961 tttccattta atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct
    49021 ttgattgtga tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt
    49081 cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt
    49141 ggatagcttc gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt
    49201 ttatgaacaa ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga
    49261 tatggtaccc gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta
    49321 caccgcttaa aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac
    49381 caaaaaaagc acttggattg tattttttga tagtcaacat attatctgta tctttcaaag
    49441 aatccttcaa aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat
    49501 cagtttgttg actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg
    49561 cagcggtacg tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag
    49621 tgtgtggaga gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag
    49681 cagtttttga taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt
    49741 caaataacaa tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa
    49801 aggattcctt tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg
    49861 cattcgatgc atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat
    49921 gtcgaagtaa aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat
    49981 gacgatattt ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga
    50041 gcaggaaagt caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag
    50101 atcattattg atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga
    50161 cgaacagatg tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa
    50221 gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg
    50281 aggcaagtgg gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa
    50341 cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat
    50401 gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac
    50461 gctagcagac aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc
    50521 atcgctgata gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat
    50581 catccttcat ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat
    50641 cttaaagcag gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt
    50701 attgtcatgc taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa
    50761 aaaacgatgg atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact
    50821 ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa
    50881 acaggaaaac agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct
    50941 tataacaaaa ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa
    51001 ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt
    51061 tatcagattg attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt
    51121 tctccattgt tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc
    51181 gattattttg tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat
    51241 cttgatttta caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg
    51301 gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa
    51361 gagctgaaaa aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc
    51421 aaagcacaaa caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa
    51481 cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta
    51541 cctcaacaag aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa
    51601 ctggcctctg cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa
    51661 ccatcatacc tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg
    51721 tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca
    51781 ttgatcacct ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca
    51841 ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt
    51901 ctagcctcag ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg
    51961 atcatttttg agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac
    52021 tggccgccga ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg
    52081 atcgttttat tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg
    52141 aagccaggga aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt
    52201 aatcaaaaaa tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt
    52261 atcggaattg ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta
    52321 agttctgttt cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg
    52381 gattcatcca cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa
    52441 gtaaatgaga tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct
    52501 tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat
    52561 ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa
    52621 aaagtagcag aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat
    52681 caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt
    52741 tatatgtccc ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt
    52801 ttgctgcgta cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt
    52861 gccacagacg ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa
    52921 tcaatggcga acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct
    52981 tttatcgtct tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct
    53041 acaataaaag tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat
    53101 atcatcttta ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac
    53161 ttcatcttgc aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg
    53221 gcttatgtct tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc
    53281 catttcaaat taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag
    53341 aggttgtgaa atcagcgttt tgagctaata acgagggtca gctgctaatt gaacaccatg
    53401 aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca aataaaatta
    53461 gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt ttgaacacca
    53521 tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca aatacatgtc
    53581 tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat aaattttaca
    53641 aacgctttct gttcagttat aataataaat gataacttgt atatataagc tgtggtaggt
    53701 aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt catttaccaa
    53761 atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt aaaaggaatc
    53821 aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct aaaccctatt
    53881 tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc catcgatcct
    53941 atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa ataccggata
    54001 gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa
    54061 gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga gccaacggat
    54121 tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg gaaatattat
    54181 ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc agtaagagag
    54241 gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt ccgtttcgat
    54301 gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa
    54361 cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa ccaagcaacg
    54421 tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac gatcgaaaat
    54481 tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa tttccatcat
    54541 ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga ttttgcagaa
    54601 ttaaagcgac tatttcatac atggggagaa aatatgagtg agggtaacgg ctggaatgct
    54661 ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagt tatcgatcag
    54721 tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg tgggacgccc
    54781 tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc gatttccgac
    54841 tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg attcacagaa
    54901 aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac acccatgcaa
    54961 tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa agttggaggc
    55021 aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat tttgtcctat
    55081 tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg agattatcat
    55141 gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga aggacagcag
    55201 ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc agaaggattc
    55261 acagaagcca atattcttat cgataattat tccgatactg ttcttcatca aagtctggag
    55321 ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt
    55381 tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata atcttcttca
    55441 gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta gcgttgtttg
    55501 aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg aataccaaaa
    55561 ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa gatcgtaatc
    55621 ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc gtggagaagt
    55681 tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc ttggaatgca
    55741 atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat acataacaac
    55801 ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa acaaacccag
    55861 tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga acccaattgt
    55921 tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg gacaatcgct
    55981 aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac aattggacca
    56041 gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg
    56101 ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc gtcaatagaa
    56161 gcaaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg tcggagactc
    56221 tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc ctgttcttca
    56281 agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat cacataaacg
    56341 ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa
    56401 agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat aatgattccg
    56461 acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc aagtaagata
    56521 actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa acgattcgtt
    56581 gtatctgttt cttttttcac tctttttcct cctttatcta taacttcatt atagacaaaa
    56641 gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat cattcatata
    56701 aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca tatgataaat
    56761 taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg gaacattcaa
    56821 atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta tattggccat
    56881 gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg aggacagtaa
    56941 agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa aagcaatcgt
    57001 cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc acgattatga
    57061 accttctgct aaagacatgg caaagatcca agatacagat atctttgttt ataatgatga
    57121 aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca atgtccatac
    57181 gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac atgatcatga
    57241 ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat ggctagctcc
    57301 aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag cttacccaga
    57361 aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc aagcactcca
    57421 tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc
    57481 tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg ctggtctttc
    57541 ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg ttaaagaaca
    57601 tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca gaacgttagc
    57661 aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga cgaatgaaca
    57721 aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag cgttgaaaaa
    57781 aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag aaaagagcgt
    57841 ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag attgggcagg
    57901 aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg tttttgaata
    57961 taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt atacgaaggg
    58021 ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat ttacgtttaa
    58081 ggatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct tgacctacga
    58141 ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt
    58201 tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac attttcattt
    58261 atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt ggccaacatt
    58321 ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc actgataggc
    58381 aaaaaa
//
LOCUS       DAZBTH010000008        51175 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000008 DAZBTH010000000
VERSION     DAZBTH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.008"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHGNW_000480"
     CDS             290..1135
                     /locus_tag="ACHGNW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6924827.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACHGNW_000481"
     CDS             1416..2636
                     /locus_tag="ACHGNW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6924828.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACHGNW_000482"
     CDS             2633..5353
                     /locus_tag="ACHGNW_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6924829.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACHGNW_000483"
     CDS             5337..6281
                     /locus_tag="ACHGNW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6924830.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="ACHGNW_000484"
     CDS             complement(6350..7360)
                     /locus_tag="ACHGNW_000484"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6924831.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="ACHGNW_000485"
     CDS             complement(7540..7884)
                     /locus_tag="ACHGNW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6924832.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="ACHGNW_000486"
     CDS             complement(7877..8311)
                     /locus_tag="ACHGNW_000486"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN6924833.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="ACHGNW_000487"
     CDS             8449..9186
                     /locus_tag="ACHGNW_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924834.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="ACHGNW_000488"
     CDS             9179..10390
                     /locus_tag="ACHGNW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924835.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="ACHGNW_000489"
     CDS             10594..11376
                     /locus_tag="ACHGNW_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN6924836.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="ACHGNW_000490"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="ACHGNW_000490"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN6924837.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACHGNW_000491"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACHGNW_000491"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN6924838.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="comGA"
                     /locus_tag="ACHGNW_000492"
     CDS             14092..15021
                     /gene="comGA"
                     /locus_tag="ACHGNW_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN6924839.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /gene="comGB"
                     /locus_tag="ACHGNW_000493"
     CDS             14978..16027
                     /gene="comGB"
                     /locus_tag="ACHGNW_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN6924840.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /gene="comGC"
                     /locus_tag="ACHGNW_000494"
     CDS             16024..16302
                     /gene="comGC"
                     /locus_tag="ACHGNW_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN6924841.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /gene="comGD"
                     /locus_tag="ACHGNW_000495"
     CDS             16277..16747
                     /gene="comGD"
                     /locus_tag="ACHGNW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN6924842.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16719..17066
                     /locus_tag="ACHGNW_000496"
     CDS             16719..17066
                     /locus_tag="ACHGNW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924843.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17029..17454
                     /gene="comGF"
                     /locus_tag="ACHGNW_000497"
     CDS             17029..17454
                     /gene="comGF"
                     /locus_tag="ACHGNW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN6924844.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /gene="comGG"
                     /locus_tag="ACHGNW_000498"
     CDS             17426..17785
                     /gene="comGG"
                     /locus_tag="ACHGNW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN6924845.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="ACHGNW_000499"
     CDS             17957..18964
                     /locus_tag="ACHGNW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6924846.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="ACHGNW_000500"
     CDS             18989..20173
                     /locus_tag="ACHGNW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN6924847.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACHGNW_000501"
     CDS             complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACHGNW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN6924848.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22298..22426)
                     /locus_tag="ACHGNW_000502"
     CDS             complement(22298..22426)
                     /locus_tag="ACHGNW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924849.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22771..22914)
                     /locus_tag="ACHGNW_000503"
     CDS             complement(22771..22914)
                     /locus_tag="ACHGNW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN6924850.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="ACHGNW_000504"
     CDS             complement(23136..23615)
                     /locus_tag="ACHGNW_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6924851.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="ACHGNW_000505"
     CDS             complement(23688..23972)
                     /locus_tag="ACHGNW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924852.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="ACHGNW_000506"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHGNW_000506"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="ACHGNW_000507"
     CDS             complement(24316..25602)
                     /locus_tag="ACHGNW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN6924853.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="ACHGNW_000508"
     CDS             25740..26411
                     /locus_tag="ACHGNW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN6924854.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="ACHGNW_000509"
     CDS             26377..26865
                     /locus_tag="ACHGNW_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN6924855.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="ACHGNW_000510"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="ACHGNW_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN6924856.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="ACHGNW_000511"
     CDS             27826..28590
                     /locus_tag="ACHGNW_000511"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN6924857.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="ACHGNW_000512"
     CDS             28823..31036
                     /locus_tag="ACHGNW_000512"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN6924858.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /gene="dtd"
                     /locus_tag="ACHGNW_000513"
     CDS             31047..31493
                     /gene="dtd"
                     /locus_tag="ACHGNW_000513"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN6924859.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="ACHGNW_000514"
     CDS             31664..32923
                     /locus_tag="ACHGNW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN6924860.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="ACHGNW_000515"
     CDS             complement(33012..33959)
                     /locus_tag="ACHGNW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6924861.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /gene="hisS"
                     /locus_tag="ACHGNW_000516"
     CDS             34353..35645
                     /gene="hisS"
                     /locus_tag="ACHGNW_000516"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN6924862.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="ACHGNW_000517"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="ACHGNW_000517"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN6924863.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="ACHGNW_000518"
     CDS             37538..38413
                     /locus_tag="ACHGNW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6924864.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="ACHGNW_000519"
     CDS             38679..40562
                     /locus_tag="ACHGNW_000519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6924865.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="ACHGNW_000520"
     CDS             complement(40637..40921)
                     /locus_tag="ACHGNW_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6924866.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="ACHGNW_000521"
     CDS             complement(40911..41645)
                     /locus_tag="ACHGNW_000521"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN6924867.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="ACHGNW_000522"
     CDS             41774..42397
                     /locus_tag="ACHGNW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN6924868.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="ACHGNW_000523"
     CDS             42589..43710
                     /locus_tag="ACHGNW_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6924869.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="ACHGNW_000524"
     CDS             43717..46845
                     /locus_tag="ACHGNW_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFN6924870.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="ACHGNW_000525"
     CDS             46864..50058
                     /locus_tag="ACHGNW_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN6924871.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="ACHGNW_000526"
     CDS             complement(50204..50911)
                     /locus_tag="ACHGNW_000526"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6924872.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16747 a         8938 c        10630 g        14860 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttat
//
LOCUS       DAZBTH010000009        47026 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000009 DAZBTH010000000
VERSION     DAZBTH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.009"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACHGNW_000527"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACHGNW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN6924873.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACHGNW_000528"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACHGNW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN6924874.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHGNW_000529"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHGNW_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN6924875.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHGNW_000530"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHGNW_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN6924876.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACHGNW_000531"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACHGNW_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6924877.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACHGNW_000532"
     CDS             7565..8422
                     /locus_tag="ACHGNW_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN6924878.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACHGNW_000533"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACHGNW_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN6924879.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACHGNW_000534"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACHGNW_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN6924880.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACHGNW_000535"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACHGNW_000535"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6924881.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACHGNW_000536"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACHGNW_000536"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6924882.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACHGNW_000537"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACHGNW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN6924883.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACHGNW_000538"
     CDS             13203..13895
                     /locus_tag="ACHGNW_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6924884.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACHGNW_000539"
     CDS             13892..14659
                     /locus_tag="ACHGNW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6924885.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACHGNW_000540"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACHGNW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN6924886.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACHGNW_000541"
     CDS             16624..16944
                     /locus_tag="ACHGNW_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN6924887.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACHGNW_000542"
     CDS             16944..17294
                     /locus_tag="ACHGNW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN6924888.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACHGNW_000543"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACHGNW_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN6924889.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACHGNW_000544"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACHGNW_000544"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN6924890.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACHGNW_000545"
     CDS             19288..20313
                     /locus_tag="ACHGNW_000545"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6924891.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACHGNW_000546"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACHGNW_000546"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6924892.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACHGNW_000547"
     CDS             21391..21831
                     /locus_tag="ACHGNW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN6924893.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACHGNW_000548"
     CDS             21834..22436
                     /locus_tag="ACHGNW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6924894.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACHGNW_000549"
     CDS             complement(22495..23601)
                     /locus_tag="ACHGNW_000549"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN6924895.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHGNW_000550"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHGNW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN6924896.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACHGNW_000551"
     CDS             complement(25149..27494)
                     /locus_tag="ACHGNW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN6924897.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACHGNW_000552"
     CDS             27773..28675
                     /locus_tag="ACHGNW_000552"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN6924898.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACHGNW_000553"
     CDS             28888..30663
                     /locus_tag="ACHGNW_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN6924899.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACHGNW_000554"
     CDS             complement(30729..32315)
                     /locus_tag="ACHGNW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN6924900.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACHGNW_000555"
     CDS             32566..32790
                     /locus_tag="ACHGNW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN6924901.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACHGNW_000556"
     CDS             32932..34683
                     /locus_tag="ACHGNW_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN6924902.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACHGNW_000557"
     CDS             34683..36467
                     /locus_tag="ACHGNW_000557"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924903.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACHGNW_000558"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACHGNW_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN6924904.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACHGNW_000559"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACHGNW_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACHGNW_000560"
     CDS             complement(39663..40565)
                     /locus_tag="ACHGNW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6924905.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACHGNW_000561"
     CDS             40796..42199
                     /locus_tag="ACHGNW_000561"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6924906.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACHGNW_000562"
     CDS             42199..43632
                     /locus_tag="ACHGNW_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6924907.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACHGNW_000563"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACHGNW_000563"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN6924908.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACHGNW_000564"
     CDS             44917..46644
                     /locus_tag="ACHGNW_000564"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN6924909.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACHGNW_000565"
     CDS             46659..47009
                     /locus_tag="ACHGNW_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN6924910.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        15199 a         8272 c        10124 g        13431 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aataga
//
LOCUS       DAZBTH010000010        46144 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000010 DAZBTH010000000
VERSION     DAZBTH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.010"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1155)
                     /locus_tag="ACHGNW_000566"
     CDS             complement(52..1155)
                     /locus_tag="ACHGNW_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN6924911.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1784..2254)
                     /locus_tag="ACHGNW_000567"
     CDS             complement(1784..2254)
                     /locus_tag="ACHGNW_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN6924912.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2584..4224
                     /locus_tag="ACHGNW_000568"
     CDS             2584..4224
                     /locus_tag="ACHGNW_000568"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN6924913.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4378..5700
                     /locus_tag="ACHGNW_000569"
     CDS             4378..5700
                     /locus_tag="ACHGNW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN6924914.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5768..6202)
                     /locus_tag="ACHGNW_000570"
     CDS             complement(5768..6202)
                     /locus_tag="ACHGNW_000570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6924915.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6267..7104)
                     /locus_tag="ACHGNW_000571"
                     /pseudo
     CDS             complement(6267..7104)
                     /locus_tag="ACHGNW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7846..8472)
                     /locus_tag="ACHGNW_000572"
     CDS             complement(7846..8472)
                     /locus_tag="ACHGNW_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924916.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8767..9102)
                     /locus_tag="ACHGNW_000573"
     CDS             complement(8767..9102)
                     /locus_tag="ACHGNW_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6924917.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9089..9373)
                     /locus_tag="ACHGNW_000574"
     CDS             complement(9089..9373)
                     /locus_tag="ACHGNW_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6924918.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9429..10952)
                     /locus_tag="ACHGNW_000575"
     CDS             complement(9429..10952)
                     /locus_tag="ACHGNW_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6924919.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10945..12120)
                     /locus_tag="ACHGNW_000576"
     CDS             complement(10945..12120)
                     /locus_tag="ACHGNW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6924920.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12124..12309)
                     /locus_tag="ACHGNW_000577"
     CDS             complement(12124..12309)
                     /locus_tag="ACHGNW_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924921.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12275..13969)
                     /locus_tag="ACHGNW_000578"
     CDS             complement(12275..13969)
                     /locus_tag="ACHGNW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6924922.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13966..14439)
                     /locus_tag="ACHGNW_000579"
     CDS             complement(13966..14439)
                     /locus_tag="ACHGNW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN6924923.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14508..14663)
                     /locus_tag="ACHGNW_000580"
     CDS             complement(14508..14663)
                     /locus_tag="ACHGNW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN6924924.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14797..15177)
                     /locus_tag="ACHGNW_000581"
     CDS             complement(14797..15177)
                     /locus_tag="ACHGNW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6924925.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15181..15396)
                     /locus_tag="ACHGNW_000582"
     CDS             complement(15181..15396)
                     /locus_tag="ACHGNW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924926.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15399..15806)
                     /locus_tag="ACHGNW_000583"
     CDS             complement(15399..15806)
                     /locus_tag="ACHGNW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN6924927.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16072..17547)
                     /locus_tag="ACHGNW_000584"
     CDS             complement(16072..17547)
                     /locus_tag="ACHGNW_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN6924928.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17537..18385)
                     /locus_tag="ACHGNW_000585"
     CDS             complement(17537..18385)
                     /locus_tag="ACHGNW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN6924929.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18422..18784)
                     /locus_tag="ACHGNW_000586"
     CDS             complement(18422..18784)
                     /locus_tag="ACHGNW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924930.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18785..18943)
                     /locus_tag="ACHGNW_000587"
     CDS             complement(18785..18943)
                     /locus_tag="ACHGNW_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924931.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19084..19708)
                     /locus_tag="ACHGNW_000588"
                     /pseudo
     CDS             complement(19084..19708)
                     /locus_tag="ACHGNW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19705..20016)
                     /locus_tag="ACHGNW_000589"
     CDS             complement(19705..20016)
                     /locus_tag="ACHGNW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924932.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20060..20353)
                     /locus_tag="ACHGNW_000590"
     CDS             complement(20060..20353)
                     /locus_tag="ACHGNW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924933.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20547..21194
                     /locus_tag="ACHGNW_000591"
     CDS             20547..21194
                     /locus_tag="ACHGNW_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6924934.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21255..22400
                     /locus_tag="ACHGNW_000592"
     CDS             21255..22400
                     /locus_tag="ACHGNW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6924935.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22481..22786)
                     /locus_tag="ACHGNW_000593"
     CDS             complement(22481..22786)
                     /locus_tag="ACHGNW_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN6924936.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22811..23242)
                     /gene="ruvX"
                     /locus_tag="ACHGNW_000594"
     CDS             complement(22811..23242)
                     /gene="ruvX"
                     /locus_tag="ACHGNW_000594"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN6924937.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23239..23505)
                     /locus_tag="ACHGNW_000595"
     CDS             complement(23239..23505)
                     /locus_tag="ACHGNW_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN6924938.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23760..24485)
                     /locus_tag="ACHGNW_000596"
     CDS             complement(23760..24485)
                     /locus_tag="ACHGNW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN6924939.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24846..24916)
                     /locus_tag="ACHGNW_000597"
     tRNA            complement(24846..24916)
                     /locus_tag="ACHGNW_000597"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24882..24884),aa:Gly,seq:ccc)
     gene            25033..25785
                     /locus_tag="ACHGNW_000598"
     CDS             25033..25785
                     /locus_tag="ACHGNW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6924940.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25801..26280
                     /locus_tag="ACHGNW_000599"
     CDS             25801..26280
                     /locus_tag="ACHGNW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6924941.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26531..28111)
                     /locus_tag="ACHGNW_000600"
     CDS             complement(26531..28111)
                     /locus_tag="ACHGNW_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN6924942.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28104..28847)
                     /locus_tag="ACHGNW_000601"
     CDS             complement(28104..28847)
                     /locus_tag="ACHGNW_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924943.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29023..29799)
                     /locus_tag="ACHGNW_000602"
     CDS             complement(29023..29799)
                     /locus_tag="ACHGNW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6924944.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30047..30883)
                     /locus_tag="ACHGNW_000603"
     CDS             complement(30047..30883)
                     /locus_tag="ACHGNW_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN6924945.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30894..31823)
                     /locus_tag="ACHGNW_000604"
     CDS             complement(30894..31823)
                     /locus_tag="ACHGNW_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN6924946.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            32015..32368
                     /locus_tag="ACHGNW_000605"
     CDS             32015..32368
                     /locus_tag="ACHGNW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6924947.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32442..33098)
                     /locus_tag="ACHGNW_000606"
     CDS             complement(32442..33098)
                     /locus_tag="ACHGNW_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6924948.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33324..35258
                     /locus_tag="ACHGNW_000607"
     CDS             33324..35258
                     /locus_tag="ACHGNW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6924949.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35344..36567)
                     /locus_tag="ACHGNW_000608"
     CDS             complement(35344..36567)
                     /locus_tag="ACHGNW_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6924950.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36561..38432)
                     /locus_tag="ACHGNW_000609"
     CDS             complement(36561..38432)
                     /locus_tag="ACHGNW_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN6924951.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38453..39571)
                     /locus_tag="ACHGNW_000610"
     CDS             complement(38453..39571)
                     /locus_tag="ACHGNW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6924952.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39654..40487)
                     /locus_tag="ACHGNW_000611"
     CDS             complement(39654..40487)
                     /locus_tag="ACHGNW_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924953.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40480..41397)
                     /locus_tag="ACHGNW_000612"
     CDS             complement(40480..41397)
                     /locus_tag="ACHGNW_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924954.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41624..42748)
                     /locus_tag="ACHGNW_000613"
     CDS             complement(41624..42748)
                     /locus_tag="ACHGNW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6924955.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            42931..43839
                     /locus_tag="ACHGNW_000614"
     CDS             42931..43839
                     /locus_tag="ACHGNW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6924956.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            43980..45593
                     /locus_tag="ACHGNW_000615"
     CDS             43980..45593
                     /locus_tag="ACHGNW_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6924957.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13922 a         9240 c         7692 g        15290 t
ORIGIN      
        1 taggagtacg gcatcatctg ccgcactcct atttttttcg tcatttattt cttaaataaa
       61 acgattctca cgcaatttta tttttttgat atcttctact gtttgtgttc cagctaattg
      121 catgaccatt tccaattctg ttttaaagaa atcaaacact tgatggactc ctgtacttcc
      181 tcctaaagac aatccataaa taacgggtct gccaatagca acaagatcgg cacctgatgc
      241 gattgctttg aaaacatgtt gtccacgtcg tacaccgctg tcaaacacga ttggtacacg
      301 tttatctact gcttctgcca catattgcaa cgaatcaaac gcagcaggcc ccccatctaa
      361 ttgacggcca ccatggttag atacccaaat tccaccagca ccagattcta atgatcgata
      421 gacatcttct tctgattgaa cacctttcac gtagactggc aaatcagaat gagcagcgat
      481 aaattctaca tcttttggac taagtttttg tttggaagat ttatatacag catccatcgt
      541 ttggcctact cctgattgat aagcctgaac gatcggcatt ggcagcggga aggtaaaccc
      601 gtttctgcgg tctgtttcac ggtttccgcc aactgttgca tccgctgtca atacgattgc
      661 ttttgctcca ttacgcttgg ccacttcaag aatatccaaa ttgattccat tatctttgct
      721 catataaaat tgaaaccatt gcggtgcttt ctccccgcct gcttctctta tctcttctaa
      781 agtacaagaa gcgtaagaac ttgctgtata gatcgtccca aaatctgcta caccttttgc
      841 agaagctttc tccgccttca catgagctaa tccatgagca gcaactggtg ccatgatgat
      901 tggtgcagtc agcatttctt cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct
      961 taatacatga ggaataatca aacgatgatt gaatgcccgt tcattttctt gataagtaaa
     1021 taagtcgcca gcaccactgc tgatgtaacc ataaccacca gctggaatca cttgtgaagc
     1081 cgcgatttct aaatcttcca tattgataaa atctactatt ccttcatttg tacctgcttg
     1141 atatgttttt tccatctcaa tgccttctct ctataaaatt tgaatgatga acaatatgat
     1201 agcgtttaca ctttttatat tactcttcga cattgataca ggcaaataat ctgtttacat
     1261 ttcagccatt ccagatgtca ctactctgga agaaatgatt gtaataaaaa tagataaaaa
     1321 accgatggga ctttgacaaa agtagtgtca aagtcccttt ttttgaataa acggtgttca
     1381 aaagccattt tctcggtatt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     1441 aaatttttct tactactcga aactaagcga ctttttcaca acctctcgat aaaacggtgt
     1501 tcaatcagct gaccctcggt attagctcaa aaaatggcaa aatatgagga actattttgc
     1561 catttgtctc tctaatactc aggtcagaac gttgacttca cagtctcttc cttaaacggt
     1621 gttcaaaaac ttgcatctgc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt
     1681 tttgtttggc tgttcttatt acttgatgca gggcaggttt ttcacgacct ctgattcacg
     1741 gtgtttcttt ttcatcaaca tttttacgaa atactgtgga agactattga aataagtgtg
     1801 gattgtttct ttgtgtttca atcgtttctt gccattcttc atttaatttt ggattaccgt
     1861 tttcttcctt gaactcataa ggtcctaatt caaaaatccc agacagtatt tgattcacga
     1921 tttcatatag ttcagtgtga tcagacacat gattcttgaa gatcatctca ttatcaccac
     1981 gtttgaacct cacattcggg ctctcatgat gaacctctat gcatttttct ccttttttta
     2041 actgacggac ttctgaaaac atctcataaa tatcttctcc agtaaaacct tcagcaagcg
     2101 ccagactgtc cacaagaccg cctactgtca gttcagagat tattgcttga ttggaatcta
     2161 attttaatgt atagcttgtc gcttctacta cgccacatgc attgcaaccg ccttctgata
     2221 gttcgctttt tctaatcaat tgaacttctt tcattttctc ttcctcctct taattttcta
     2281 cttatgtatt tttatcacct gacataggtg actcctatca acgctatcat gtgctttttt
     2341 cctcagaaat cctatttatc cattccatct atcccttatt cttctacttt tctttctcta
     2401 gcatataaga gatacgcata gtattcaagc aaggattgtc gatataaaaa gaacaaatac
     2461 tgctattttg atttcttttg gctatattac atataaataa aatttatcat acctgctctt
     2521 tttgactata tcatcatctg acttctatac ttcgtttgta ttaaaactta ggaggaatca
     2581 ttcatgctta cagggttaaa cttattaaat aatccttttc taaataaagg aacagctttt
     2641 acaaaagaag aacgggcaaa atatggaatc actggtatgc ttccaagtac tgtccaaaca
     2701 ctggaacaac aatctgttca agcttatggt caatacttaa gcaaacaaac agatttggaa
     2761 aaacgtatct tcttaatgaa tctattcaat acgaatcgga cattgtttta taaattgatg
     2821 ggcgaacatt tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc
     2881 gaacaataca acgaaatttt cttgaaaccg caagatgctg catttctttc aatcgacgaa
     2941 ccagaaaaca tcaaagcaag tttgaagaac gcggcagatg gacgagacat tcgtttgatt
     3001 gttgtgactg atgcagaagg aatcttaggt atgggcgact ggggtgtgaa cggtgttgac
     3061 atcgctatcg gcaaattgat ggtctatact gctgctgctg gtattaatcc agcacaagtt
     3121 ttgcctgtat cgatcgacgc tggtactaat aatgaagaat tattgaataa tcccttatac
     3181 ttaggcaatc gtcatgctcg tgtagaaggc gaaacttact atgaattcat cgatcaattc
     3241 gttcaatcag ctaccgaatt attcccagaa ttgttattgc attgggaaga ttttggacga
     3301 ggaaatgctg cagcgatctt agaaaaatac gaagatcaaa tcactacttt caacgacgat
     3361 atccaaggta caggaattgt tgtcctagca ggtgtattag gcggattgaa tatatccggt
     3421 gaacaattga aagatcaaat aattttgaca tttggtgctg gtactgctgg tgtgggtatc
     3481 gcaaacatcc ttttagatga gatgatccgt caaggtgtac ctgaagaaga agcacgccaa
     3541 catttctatc aagtagataa acaaggttta ttgtttgaag atacagaagg attgactcct
     3601 ggtcaaatcc cattcgctcg caaacgctca gatttcacta atgctgatga attgacaaac
     3661 ttagaagcag ttgtaaaagc gatccatccg acaatcatga ttggtacttc tacccaacct
     3721 ggagcattca ctgaagaaat cgtgaaagaa atggctgctc atacaccacg tccgatcatc
     3781 atgcctttgt ctaatccaac aaaattggca gaagcaaaag caaaagattt aatagaatgg
     3841 acggacggaa aagcattagt tggaacaggt atccctgctg ctgatgttga atataatggt
     3901 gtaacttacc aaatcggtca agcaaataat gctttgatgt acccaggttt aggacttggt
     3961 ttgattgctt caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca
     4021 ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa
     4081 atcactgaat tttctcaaac gattgctgaa actgtcgcaa aatcagttgt cgcacaaaac
     4141 ttgaatcgtg aagaaatcac ggatatcaaa gaagcagttg aatctgctaa atgggttcct
     4201 gaatataaat ctttagaaga ttaagatcgt tatagtaaaa gaacatacag aaactttgta
     4261 tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagcg gccagccaac tcctgctctt
     4321 ttgtcgcttt tctttggctt ttttcatatg atacagaata agaaaggatc tgtaaaaatg
     4381 gcacaaaaaa caacaactgc aactgcagtt gaggaaaaga aaaaagaagg tttttggtcc
     4441 acaaaaatca ccggcgtaag tttgccactt tatttgatca tggtccttgt cctgatcatt
     4501 gcaatggctt tagggaaatt accgacgaat atgattggac caatcgcagt tttggttatt
     4561 ttcggtaatt tatttcattt tatcgggaca aaaatcccta tcgtcaaaag ttatttgggt
     4621 ggtggttccg tttttgcgat cttcgcttct gctgcgattg ctacatttgg gatcttacct
     4681 gaatatgtag tgaaatccac agaaaacttt gtcagcaata tgggcttttg ggacttttat
     4741 attgctgcct tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct
     4801 tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga
     4861 ctcgtgggca tgcttatcgg aaatggtttt gcaaattccg ttttatacat ctcattacct
     4921 gcgatggctg gtggtgttgg agccggagcc gttcctttat caacgattta tgcaaatgtt
     4981 ttaggtacgg atgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat
     5041 gtcttagcta tcatcggtgc agcattagtt gctcgtgctg gacaatcaat gccaaaattc
     5101 aacggcaatg gtaaattaat ggaaaaaggc gatcttggcg atggaaaacc tcgaactgtg
     5161 aaaccttcca ttccacagtt aggtgttggt ttgatggtat cattgacttt cttcatctta
     5221 ggacaaattg gtaactattt tgttcctaaa gtccatgctt acgcatttat gattattatc
     5281 gttgtcatct gtaaaatcgc caacctatta ccagagtatt atgaagatgc agctatcatg
     5341 ttcaataact tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg
     5401 ttgctgaatc tgaatgtatt agcttctgct ttgacatggc aatttgttgt cttatgtctg
     5461 acaagtgtta tcgcgatttc agttgtttca ggatttgtcg gtcgtttgtt tggtttatat
     5521 ccaattgaat caactattac tgctggctta tgcaacaact cgatgggcgg aacaggaaat
     5581 gtagccgtct tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat
     5641 cgacttggtg gtgccatcgt tttgatcatc tctggtttct tgatgcaatt actttcttag
     5701 tcaaaaaaac aaaaatacct gatcatcaag tatcggcttg gtgatcgggt atttttttga
     5761 taaagattta caggattttc aaaaatcttt tttgcatttt atcggaatga taaccgatgt
     5821 gttcataaaa ttggtgtgct tctactctgt attctgcaga attaagcctt attgtcgtta
     5881 ttcctcgttt tttcgcttct tcttctacgc cagtcatcaa agcctttccg atgcctcttt
     5941 tttcagcata tttagacaca gcaagtgcca taatattgaa cattggatcg ctaaaaatcg
     6001 attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga tcttcataaa
     6061 ccaagaaaaa atttttctct ggctccttca gcagtttctt catttgtctc accgtaacgg
     6121 ttaatggtat atcataccct aattgatccg cattgatttc acgtagtgct tctgcatctt
     6181 ctaaacgcat ttcccgtaac atccaaatca ccagccaatt tctgattttt taaaatgaat
     6241 ttttttatca tagtatactc tttttttcat ttagaacaat atgtttcagt gattttgact
     6301 tgcgttagtc tagattctcc taatgtttgt aagatgacaa aggcattgta acaattgacc
     6361 aaaagaacca cgaggacaat tacttgaaaa ataaaggtca ctttttttgg agacaaaacc
     6421 ctgctaaatc ttgctcccca caaacctcca ataacagcag cggggataac ataaaacaac
     6481 atagataaat caaagcgcat aaagctactt gttaaagcaa tcgttaccaa ttttgccagt
     6541 tgagaaaaga aaatcgtaga tagagaatat agtgttgctt cttttattgg caatgcaaac
     6601 atcagcatca acaaagaaac attgactggt ccaccaccaa tacctaaaaa actagctaaa
     6661 aagcccagaa ccccccccac atataaggta ccaagtccat gacgtcaaat gaaattttgg
     6721 ctgatgatgc ttggtgtaga agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg
     6781 gatcagctgc acatgcttct cgttttcaaa taactgtaaa aaaacttcga atacaatgtt
     6841 gccgactact cctccaacaa cagctccact agatacccaa ccgacaatct gccaattcag
     6901 tgatttcccg cttgatactt gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac
     6961 tgttgagtaa aaagaaatcc ctgcaacaga atgggcgccg ataagatcaa gaatcggttt
     7021 gatcaacacg cctccaccca tcccggaaat agcgccaact gtgttcgcca gaacgatcac
     7081 cacaaaataa atcagaccaa tcatcttgta tttcctttct cttgtttata tcttttagcg
     7141 attgttactc ttttctttta ttatacagta aaagaaacaa acgaggcttc gaaagctttt
     7201 ctacttttta aggaaattta gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat
     7261 caagtaataa gaacagccaa acaaaaatag ctcctcataa ttttcgtttg gttgaactta
     7321 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca
     7381 gctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattgtga
     7441 cttgtgaccg aggagttggc aggagaacac cgtttaagga agagactgtg aagtcaacgt
     7501 tttgacctga ttattagaga aacaaatggc aaaatagttc ttcatatttc gccgtttttc
     7561 gagcgaatgc cgaggggcag ctgattgaac atcgtttatt tggaaaaagg gattgtgaca
     7621 agacgtttgt cacaatccct ttttttatat cttattaatt cattcttttg ttctattctt
     7681 cttcttccgc catgaatgag aaacccatat caaagaacta taaaggttat caaaacagcg
     7741 tttatgacta aatgaattag agttatctat cactattttt caagcgattt aaaccaaatg
     7801 gcatacaata tggcatacaa aaacttcaat aagccgtggg ggtttttaaa tttcttcttg
     7861 ttctttaaaa tcaatgagtg gagctacata atttgaatcc actttttcaa acgtcacttt
     7921 ttgcatcgac gaatgttttc tttcccaaga taacgtaaat agatttgact ctggacttat
     7981 aaagtctact tcaccttcta gtattacttt cttatgcaaa tattcctcta ataaatatag
     8041 acttgctaaa gcatacaaca cattttccaa atttgcttcc ttataatttg aagaacgacc
     8101 atgttttact ctgttatata ttttccacca atctggtgta tcttcaggcc acccatctcc
     8161 aaaaggtttt agtgaagtca atgttaaaga attcataacc ctaacaccat tatccaaaca
     8221 cattaattcg ctagatttaa taacagcttt aaaatcagtt atgcttttat tgctactgct
     8281 atcaaatccg caaagttctt tgaaaataat ctcacactcc gaaccaatcg ctagtagtag
     8341 tttttgatat tcaatagaga atgttttgaa attattctca tcgagcatca caaaatctgt
     8401 ggtattaata aactcatttt ctaaatgtag ataatacttc caataaaaat ttgtaaactc
     8461 tgctttgtcc attaaatcac ctcttttgaa ttataacaaa aaaaacacgt ccacaacaga
     8521 cgtgttcttt gatttctaca caatcagctt gccaaaacca tccaatcagt cctagcatgc
     8581 ctgtattaaa attgaacgtt catcatctat catctttctt agtccagcat gtttgcaacc
     8641 acacctacaa ttttcagtcc aacaatagat ttcgactagc taagtcttgc aaaattcagg
     8701 cagctaaccc ttcatttact accttaatta tactaaatac ccacaacgtt tacaagtgta
     8761 atcattctaa gaaatctgtt tagctatgat cgttgtataa tctttaaacg ccgttccttc
     8821 attgattgaa ataattttat aactcttgtt tttccaagct actttcatat cgtttgttag
     8881 ttcataatct tgatcgtatc gaataataaa cgtcaacgtt ccttcaagaa tggtacctac
     8941 actagcaagc acgtctttta gcatttgtga acgcacatta gcccaacatt caaatacagt
     9001 ttcttcacta tccaccatct gcccatcttc atcttttacc cgttttgtct ttttaaactt
     9061 gactctctca gtcaattcat ttacattctt aatgattggc ataataagca cctctcattt
     9121 gttgtactaa tgcttgtacc gttactggta tccgttcgct gctggcttct tgacggttca
     9181 aataccaatg ttgagtaagt aatgacaccg ccaaatcaaa ttgctgataa ttatccatat
     9241 ctttatccgt tgcgctacta tcaatagcac tttttatata ttcgcctgct gtatcaatca
     9301 attgttgaat aaagtcatca tcaatagtgt gatcgatccg catacttttt tttatgctat
     9361 ccaaaattac cattttatct ctcctaatga aaaaaaggca gtgggataaa ccgccgccca
     9421 atatttttct atacttttgg tgttacaact ggcgtaaact caatatacac tgaagcattt
     9481 tgatcaattt tcttataatc gttgcgaacg atcactgcaa gtccttgtga gtagtaatcg
     9541 aatttttccc attgagtcgt tacttgattt ctacgagcaa caaagataga ttgtgcaata
     9601 tcccccataa tcatagggaa tgttccagct ttaggattgg caaacaatgt atctgcaatc
     9661 aatactactg gcataccgaa taatgattta ccactaggtg ctgtcacatc gggttgtaaa
     9721 atgtaacgtc catctgaatc tttcaatgta tctaggtggt aatatccgct ttggtttaga
     9781 attaccattt tatttaatgc tgggtctaat gccacattgt atagttgttt caaatcgtcc
     9841 aacgtagcag ccgttttctt agtgaatgtt tttaataaat ccatgatatg cttattgtca
     9901 gtgttttcta ccaatttcgc taactgatct ttgacctctt gtacaatatt cactgctgaa
     9961 tcttccacaa cctcatttga taaggcaatc ttaccagcac gagtttctac tttataatca
    10021 actgaagtaa acatttcagc gtcaatatca ccaatttcag caagttcagc ttttgttgct
    10081 aacactgctt gttggttagt tgctactgga tatttacctt gtccatttga tactgttttt
    10141 actgttgcat attgagctaa gttataattt gaacgtttca aatcaaagac ttcaccgatt
    10201 acttcttcgg gaacaactgc cgctgcattt acagtagtga ctccatcacg cacttcgcct
    10261 tgtgaacgga tatattcttc atacccacgc aattctgttt tttcgttatc gattaaagtt
    10321 ttttccatgt tattactctc cttttgtgct ttagaagtgg attctaagct attgtttgtt
    10381 tgttttgctt ggttgttact cataaacgat tcgtaagaac gcttatttac ttggacattt
    10441 gatgaatcat aagcgggaac ggtcacgaca ctaatttcat taagtgcttt gattttatct
    10501 attgatcgtt caattgtgcc atcttctttt ttgtcgaatg aatctaatcc aagcacgaac
    10561 ccaaaggaca tagaatcaac aacccctttt gagatattct catacacatc atttgaatag
    10621 gtcgtgtcgg ttagctctgc ttcaaaatgt aatcctgtat catcgacatt caatttcaac
    10681 gtacctgctt tgacactagc tagtggcttt gaataatcgt gtccatataa taagaacaca
    10741 tttgataaat ccacctcttt taatgcttcg ggtgtaatca cttctacaaa gccgcctaaa
    10801 tcttttgacg gttgcccgaa tttcaacgca tagcctgaaa tagttttgcc gtctgttggt
    10861 tgtttttcat tttctttctc tactttggga gaatcggctg ttagctcagc ttcttctgtc
    10921 agccgttttt ccttttcttc ttcattattc aactggtgta actccttcct gttcttttaa
    10981 ttgatatttc tccaacgtat ctaaatacgt aaagttcaaa cttgctagta aacgatcgcc
    11041 accatccatg gggggtagcc ccattttcga ccgcccctca ttaatcgtga gaagtgaccc
    11101 tttgacctga tccaatacat tttttacttt tgtttcagga tcagtctcta gtaactgatc
    11161 ggaattaaag cggatattgg tttcaagttt cgtatctaat tcactcacaa acacattaaa
    11221 gtaatgaatc agcgtacttt ggatatactg gagattactt tgaacggtgc tagaatgttc
    11281 attttcaacg cctaaacgct caatcggtac accaaacgcc ttggcaattt gttttgtgtt
    11341 ccaatcattg gaattgataa gcttcaaaac atcggtgttt atttctaatg ttttgtagtc
    11401 cattgtttca tcaagaatga tcgttctaag agcattatct ccactacttg aaccgttggc
    11461 ttcttcaaac ttttcacgaa ttgcgttctt tgctgatccg tctaaatctg acttatgaac
    11521 cttcaaaata cctgacccac tgacaccacg agtaaagaaa ttgtgcaatg tgcggttgcc
    11581 agcttgttga attttcagtt catcacgtaa cgcataaagt ggtggtaatc ctgttaaacc
    11641 atcttgggtg aaatatttga agtgcaaaat atcgctagac ttcacacgtc tttttttatc
    11701 accaatctga taagacaacg taccattatc taattgagta accgttacac ttgagttagg
    11761 taataagtga atttcttcta ccttttcacc gttgcgttta atctctgcaa agctattacc
    11821 gtttagcaac atattgaccg ccaaagcaaa ctttaaatgc caaccatcca tttctgaatt
    11881 aggtttctcg tttagtaact tcaccagttc attatcagct tgtggcatat tgtttttgac
    11941 caattgaatc ggacttgaag caatatcact agcaataatt cgaacggctg caaacacatc
    12001 actatttctt aatgcacgaa cgcttgtaaa gctggtggtg taatcatccg attggattga
    12061 taccacatgt tgtaggaaag gatctcccgt tgtattttgt ctcgctttaa aaaatgccat
    12121 cttttaacct cccttctgtt ctgtatttag catgaccgct accccaataa gcactatgcc
    12181 cagcgctaac aagccatagt agacatttgt tagtaaggtg atagcaacca acacacacac
    12241 caatccaaga agtaaaagaa cggtatgaat gtaattagaa agaaaattct tcacttgtat
    12301 aaaattcatt gtctgctttc gctccttccg ttgtggtgaa gtaatccatt gcaaacacat
    12361 aggcattgat taatgcagct attggatcaa ttttgttact gttctttgat ttattaatct
    12421 gaatgccgtt gttatccgtt tttaaaatag cgttgttcac tgcatgggtg agaatcgtat
    12481 ttttattgtg aataacgttg ccttcataaa cttgttctct aaaagttcgg gtcggaacgt
    12541 ttagagtaat cgttccttgt cttacttcta acattgggta gttggctttt tcagctttac
    12601 tgataagcga attagcgtta tacgggtcat aacaaatccc catacattcc aaatcatttt
    12661 cttgaatcaa atcttgaata aactgaaata cttggtcata gtccacgatt ccactttcta
    12721 attgagtaat ggaacactca ccagcttttt ctagttctcg ataaggcaaa ccgtcgcgct
    12781 tttctttgtc ttgaagccca tatttggtgg ctacaaagct atgtgaatca caataaagtt
    12841 cgccgttatc tagcggtacg atccatgaaa cgctagttaa atcatctgtt ttagataaat
    12901 ccactccgat ataaacgggc ttgcctgtaa tatcttgtgg ttctacttcg gttgcctgcc
    12961 agtcttcagc aatcatatag ctgtcctcac tcgcttgtct ccataaattg aagtttttca
    13021 ccaatacaga attaaggtta ttttgtttca aagcaaggtt cacatcatct tgaatggttg
    13081 gaatcattac ttttttgatt tcttcacttt caaaaatcgg attggctttg atccatttct
    13141 cttgatcgtg aatttcttcg ggatcgtcca attcccatat cgctataaag tacctgtcgg
    13201 cttgttcttc gccttttagc acacgatcta acaataagta ttcctcgtac ataggaacgt
    13261 ttaaatcaag cccagcggta ctaataacac acaataagcc gttcttttgt tgggtcattc
    13321 ctgacttaat gacgttgtac gttttacgag tttttgcttc gtgccactcg tcaagaattg
    13381 cggttgttgg tgcaaaccca tctagcgtgc tggtttcact agccagtgcc atagcgaacg
    13441 aattagaggg caagtgctga atcttagagt tcataatttt cagttgtggt cttaaaaact
    13501 tactagactt gaccacgttt ctaagtgaat tagacagcat atcatagccc aatttagctt
    13561 gtttcaaagc gttacttaca aacaatactt gccttgcttc tgctggttct tgttccataa
    13621 tcaacgcatt ggcagccatg ccacttgcga tatacgtttt cccattctta cggctcatac
    13681 tggtaaacgc tcgattgaat cgcctgtaat tcccttcttt agtacgccag ccataaagac
    13741 tgcctagaat gaatttttga aatcctaaca tcttgattgc tttaccgtcc gtagatggta
    13801 gcaactcaat gaatttaatc gctttggtgg cttgttcttc atcgaacaca taagggaagc
    13861 tatctgactt agaacgctcc aaatcgctta tatgacgctc acacgcttgg ataattttct
    13921 tgggtgcaat cagcttacct gataggactt tctcaatgta atcattcatg aattcatcaa
    13981 ctctttgaac gggtcttcgg gttcatcatt tttaaccttg ttcaaagcta gttttgatcg
    14041 tgattctaag gtcataccta gtgaggtggc ttgttttttc aaatcactca tagcttgtga
    14101 ttgaacccga accgctggat tggctttctt tactccgttt tcgcatgtga taaacgtacc
    14161 tgattttctt atctcggctt cggctgtttt cattcgagca aaggctaaac aataggctac
    14221 caatgaacca taatctgctt cactcaatgg ataatctttt tttaatgttg gtacgatacg
    14281 attccattca cttctagcgc tcgctgccat ccagtcaggg ggcgtgatat ttattaatgg
    14341 ttgctgcttg aacaattctt ctctagcgtc cacacggatt gctttttctt cacttgatat
    14401 attgcctttt gtatcgtgta atattttagg gtgtcccatg ttgtcctcct ttcagacaaa
    14461 agaaaaagga acgtaagcaa ttaagctcac attccttcgt ttttcgatca gaatttttct
    14521 ttatctaatt tatctatata aatgattttt ccgttctgca tttgaatggt cacttgacca
    14581 aaatgaggta actcaatggt gcgctgtttc cctgttttat cagtaataac aattgtattt
    14641 ttcaataaat ctcctacttt cattatatac tttttcactt gtgattacaa gtgtaatcgt
    14701 tatttgtaat tacccttatg ccttttcaca acggttattt gtaccaaaat gagtaggcat
    14761 cgattgtcag taagtttcac actatgcccc cttaactcaa agggtactct tttctcgtct
    14821 ttgtctttca ttggctgtct tggaattatg gtgtgcatgg cataaagaac gaaaattagt
    14881 ttcatctaac cgtctattcc aatcatcttt tagttcaatt ttgtgatcta caacatcagc
    14941 tttccttatc aagccttctt ctaggcaatc ctcacatact ggatgattta accgatacaa
    15001 gtatgaagcc tttcgccatg ttctactttg atagaattga aaatatttac cttcgctttg
    15061 ctttctttct tcacgcttaa ttgccgtttg ctttgcttta tgcttcccac aatacttctg
    15121 cctataatca atcaatgttt tacatccagc atgattacat tgttttttaa ctgccataga
    15181 ttaccaacca aagcgatctc gttcgtcaga atcatcttgt tcccattttg atttgtattc
    15241 aatataaggc gaaactatct tcaagcacgt tccgtcttta aaattaactt tagtgatttt
    15301 atcttcctca aaactaataa agtctacatc ttcttgagtg tttgaatact caatgtgttt
    15361 ctcaccatcg aagtatctaa tactttccat atgcataatt attctacctc caataaatta
    15421 gttatcaatt catcatctgt tagtagcaaa gccaagaact tatcatggat gtttaaaaac
    15481 aaattatcct cattcgatgt aagctctcgt tgtctcgctc gatcatatag gttaagcaga
    15541 caattaaatt catccattag ctcacctgta tcatcatagt tgccactatc taccaatgat
    15601 cccactacta gcgattgaca ccacgctact gactcctgca gccaatcagg ttcttcttcc
    15661 attatcttta atacttctgg atcaaatgtg gaataatctt ctgttaccaa tcccacacta
    15721 aatgccagcg tgttcattag aaaccgatcc atttcatctg tatattgaaa gttcggtgct
    15781 ttgtttgttg aaataatatt gagcatttac tttcctcctt gattcgtaac acgtggtaac
    15841 acctacgtaa caggtacccg ttaccactca atcccaacgg ggacaagggt tctagcgttt
    15901 tggtaacagg taacgggtaa aatccagtaa cattattccc cagcatatta gctaactatt
    15961 tcaaaagttt taaggaaata aggtgttacc ccgttaccaa attgctgtat cccttggggc
    16021 agtaagggta aactagtaac gggctaggtg ttactgaccc gttactaccc gttacttttt
    16081 gtactttcca gctcgatttt tattttcata ggcatttgac ttattgtaaa atcctcgtcg
    16141 tttatttctt tttggcagtt gcttccgttc ccaaccagga atactgctaa taatcaaggc
    16201 aatcttttta gtttctgcat ctgatcgacc attcaataaa tcctttgctt gtttatcaaa
    16261 aacagcttcc aaaatatcag cggtgagtac gctatcaact aaataaaact cttgttgcat
    16321 agtagctcca ttatagaaat gctgtaaatt tttctcatta catttactgt caggatagtt
    16381 ctgataataa gaacgtctcg cccacgctgg agcttcttcc catttatacg ggatttccat
    16441 ttccaaatat ttagtgattg cttctttaat tggattctcc atcttggcat cctcttgata
    16501 gtcttttgca atttctaaga cttctttatc ttcatcggga ctaaaataga ttcgttgtcc
    16561 tttatcaaaa agcacctttg cttcagctaa cacttgaaga aaatattcat cctttgtttt
    16621 aaacggattc atctttggct tattcttaca aggcaaagga taaaaacggc ggtttcctgt
    16681 atcatctttc aaatactgcc catcattcga cgttccaata aatacacatt gccttgccca
    16741 agctatgaca ttccgaccat aaggaggtct aattttatcg atcttggcac tgatgaagtt
    16801 ttttatctgt tcaattttag ttttgttcat tgaagataat tctccaagtt caataattac
    16861 attgccttga agctgcatat aatcatcttt cttttcacct agcgaatcta atgaatcggt
    16921 gaaaaaatca tccgtgtaaa gtaatgctgg aacggtactc ttaccaatcc cttgcggtcc
    16981 atccaaaact ggaacgatct caaactttat tcctggttta tagatacgtg cgactgatcc
    17041 gactatccaa cgttttgtta cttctcgtgt gtaaaggttg tcgtctaccc ctaaaaggtc
    17101 aataaacagt gtttccagtc taggctgttg atcccattta actttttcga tacgttcttt
    17161 tactgggttg aaaacattct ttctagccac atttactact gctttgtgta ttttgttgtc
    17221 atcaaaaact acattgtagt tttcttcaat atatgccgtt aaggcatcct caaaactgtc
    17281 ttcccattct ccagcggtac gattgatcca aggagaatct tttaataggt gaatgtatcc
    17341 gctaaattcg ttataagcaa ttgttccagc taaattgcta tcattggtta aaatcaattc
    17401 tgcattcaca acactgttac ttttaatctg atcgtcttta ttgcatctta atcgtgacat
    17461 ccaggttttc aattcaatga tattgtcttc acgctcgctg gcttcgggaa agtctaaaat
    17521 actatctaat ttatgcttat gagccatgag ctttccccct caaatctttt ttaatcatcg
    17581 atcgaataat ttgattcact tctttatctt ttagcggtgg ttcattgtac gcatttgctg
    17641 tataaatcaa ttctgctaaa gtttgattat ctacaccgtg ccatagtaac caaccgcata
    17701 acttagttaa atgatcgttt ctactacctt cgccagcgcc ttgaacaatc atatctaatt
    17761 tttttgctgt ccctgttttt gctacgggtc ggtattgagt ctttatttct tgcggttttt
    17821 ttagcagtag gttcactagc caatcgggag ctggtgcaat attatttaga gatacgtttt
    17881 caataggttc atatgctctc ccaacacttc ttgaaataat catagttggc gctattggaa
    17941 ctcctttagt gataatatca atcccgctta aacttttacc tttatcatca cgcccgaaga
    18001 tatcagcaaa tgcacctgtt tcttgttgga taattacacc atcaggaatt ttaaagaaga
    18061 aatggattcc tttacctggt gtgatttccg tataagtaga aggaaatgga tcatacattg
    18121 aagcaagttt tttataattc tcaacgccgt ttcgtgtttc agaatgttga tctaggtcaa
    18181 ctaccaccat ttgatgatct tcaagcatta acccaatatt tctagttgca tcttccgtcc
    18241 accattcatt aatttgatta gaatcgttag ttgcatcatt ccaaccacta gagcctttta
    18301 ttggaatttt agttccagga gataagggat aaattgacca tcccgacttt tgtaattccg
    18361 ttgctgcttg ataattattt gccaataaac ctctcctcct ttttcaaaat actggtataa
    18421 atcattcaga cacctccatg agcttcaact gctcaagttc gtatttcaac ccatcccgaa
    18481 caaattcagc accttttaat tgattctgat actttaccca tgtattaaat gttttcaccc
    18541 ttgcttcttg catatcagta atgtttaacc atgcggttaa aatctgattt tttagctcgt
    18601 tgatttctcg acaaattatt ttgtgttctg catgtttttt gagtacatac aaaactgtta
    18661 gtttttcatt gaatgaattt ccaaagtagg ttttcccgat attattttct agttcagcga
    18721 tcccttgatt aatttcctct aattcaaggc tatcgagttg gtttaatagt tttgttttgt
    18781 acatctactt atgccctcca gttcttaatt gatagtcgta ctcgtcataa gcagtgaaga
    18841 gatataacgc taatcctgca aagatagcta tttttgcaat taatggtaat aagccgaaaa
    18901 ttccgccgat taaaatgctt cctataatta agtttcgttt cataatggtt ccctccgatt
    18961 ttttaatatg gtataatcgg aacaaagaat atcgggtttt atcccaatta agttgccttt
    19021 atcctgtccg ccaagactgt tatggataag gctttttctt tgttctcgat catgttaatg
    19081 ctcctaagtt gtctgcaatt cagcagaacg attataaatt ttctcactca ctgaaaactg
    19141 caacccatat ttttctttaa cagtcattaa ttctactgtc tcatctaaaa taggttcacg
    19201 atcaaccaac atttcaggag tcatatcttt cttttttacc attttggaat gaccataaat
    19261 agttgataca gctttattgg ctatcacatt cgctttaatg tagtctactg gttttggttc
    19321 agtaatagcg tggcttaatt tagtcattgc ttctttttgg tgttctttgt ctagcatgcg
    19381 gaatacttga aagccttcaa gtcctgtgga ctggcgtagt tctttgatga tgtcgaaaat
    19441 ccagtcttga aatgcctcgg cttctttttt acggcttttg aatgccatgc ggtaaatatt
    19501 ttttttcgtt gatgatgttt acatcttgtg ttctgcccaa tgaatcgatg accttactag
    19561 tagtaacccc atctttaggc aaagagtgaa tggctctagt aacctgttta agacctaacg
    19621 cttccgcaac atctttcgcc actgcccacc attcatcatt aatatcgaca aatcttataa
    19681 tgtgtccgtt ccaaatttca gttttcattc aacgtcctcc agttcaagcc ctagcaagct
    19741 cgctaacaac tcaacttgct gatacaaaat ttcttgatac tcttcaatag aaagtcgttc
    19801 tttaaactct gcttgtacta aatcgttcaa gccacgcaat actccgctta ccgttggttc
    19861 ttgtggtagc aatttttgat agtccatttg ttcgtacaaa cattcaaacg tttcttctag
    19921 cgctttgcct tccaagtttg ctaattctgc cgctgtgtct tctgtaagaa ttgttgataa
    19981 tactttttcg attgctttat tttgtgtcga ttccattatt atttcctcct attaatccct
    20041 ttgcccccac ggcttgcgtt tatttttggt attcttccat aaagcgatca cattcatctt
    20101 gtgaaattct tacgactcca tcaactataa tcaactttaa accgttagga acatatttct
    20161 gcatcatagt attgtatgaa acgttcatat acttagcagc ttgtgccttg ttcatatatt
    20221 ttggaaactg gtactttttt tctaataatg aaagtaacgt tccttgagtt tgctctactt
    20281 gttgaacaac tcgatcgtca ataattttaa aagctggatc atcaagcgtg agtttgtcaa
    20341 tttttaattc catatttatc ccccctcgtt agcatacatt tctatacaaa gattttaaaa
    20401 aaatgcaaga ggctcttgaa tacatttcta tacctcttat aaactcaata taccatcata
    20461 ccttttcatt tgcaagaatt ttaacatact tttttgaact tttatgtttc acacatattt
    20521 aaatcgtgat atagtagaga aaatatatga atggtggtga aaatgtggct agaaaaaaat
    20581 accccgaagc agtggagaca ggacaaagaa ttagactggc tagaattgaa aagggactct
    20641 caatggaaca acttggaggt aaattatccc caccggccag taaaggtgcc gttagtaatt
    20701 gggaaaatgg ctataatctt cccaataaca ccaggcttaa acaactttca aaggtattag
    20761 atgtatccac tacctatcta ttaaacggat cttatacatt aagagatatg catatgatgc
    20821 ccgaaattaa gcgaaatgtg cttgaaaagc aattgaaaaa aactattgaa aaaaataact
    20881 ctataattga ttctaatctt tctaatgcta tcaataacat cgaagaagat aacctgactg
    20941 tctcacagaa gatttattta acaaacgcaa taaaatatct taaaacagga tctcgaattg
    21001 aatttcctga cgggagtacc ccaattgctt atctcgcagc tcttctaaat acgcttttac
    21061 gatatgactc attaattaat gattcagaaa aaaatacaga tgagaaaaaa gagtatcaag
    21121 aattttatga aaatgatctt ctaaaagagt tcacagctct tgttactgag ctaaataaac
    21181 actattcaaa ataataataa actgcctcct ccaaccgccc ccacggcttg ctacactatg
    21241 gagggaaaac tattatggca acattcaagc aatatgaaac taaaaaaggt aaattttggc
    21301 tctatgaagc ctatcttgga atgaacaaaa tgactggcaa acctgataag gttcgtagac
    21361 gaggttacaa aacaaaaaaa gaagcccagc ttgccttgtc acgcttgcaa gtggactacg
    21421 ataaaaacgg tattaaaaaa gcgaacaacg aaacgtttaa agaagtttat gacttgtggc
    21481 tagaaacata taaaaccact gtccgagaag ccaccttcat gaaaactgaa atcaagttta
    21541 gaaagtggat tcttccaaaa tatggaaatc ttcgtgtgaa tgaagtcacg gtaaaaaaag
    21601 cccaacaaat tgtaaatagc tgggctgaaa ctactgacca atacaaggta cttcattcaa
    21661 cttctaagcg tatttttagc tacgctatca atcttgggat tattgagact aatccactag
    21721 acaacgtatt aatgcctaaa agaaccacca ctaagcataa agaaaaggtc aaagtttaca
    21781 gtaaagaaca actgatcacc ttgtttgatt acctaaacag caaacctgaa acttatcgaa
    21841 acgactttga taaaacgtta ctacgctttc tcttttatag cggggttagg atcagtgaag
    21901 cccttgcatt gaattggtca gacattgatt ttcaagaaaa tacggtcacc atcaataaaa
    21961 cactaagcca atccaaaaat ggctataaaa tcagtgatcc taaaactgat tgtagtgctg
    22021 gggttcttcc actagatgaa agaacaattt ctatattaaa aaaatggcag attaatcagc
    22081 ggaaatatat gctgacactt ggtgtaactg atccgactat gattttttgt ggtatctata
    22141 aagaactaat cacacaccac gctatttatg caagattgat tactatttgt gaaaaagcta
    22201 tggttccatt tctcggtgtg catgtagcaa gacacactca tgcttcaatg ctcttggacg
    22261 ctggagctac tatggtagaa gttcaaacta gattacggca tacaaaaatt agtcagacga
    22321 tcgattgtta cggtcacttg gcaaaagaaa cgaaagaaaa gactgttgaa aaattagtac
    22381 aacacttaaa tattgactag atggcataca atatggcata cagtaaaaaa taaaaaaagg
    22441 agaaacccta tcatatcaag gtttctccta gctttatagt ctattcttct tcttccgcca
    22501 tgaatgtatt gaatacttct tcaatcatgt cccattctgc gtctgtttcg atttgttcta
    22561 actcgccttc tgttccatct tcgttttcga tgtatgcata agcttgtaat tctacatctt
    22621 catcttctga aacaccagca ggatataaaa gaacatagtt acgtccaaat tcttcttgtc
    22681 cgtcaactgt tagtaggatt tcatataatg tttcatttcc ttggtcatcc accaatgtga
    22741 tgtgttcgtg tccttcatgg tcgtgggaat ggttatgttc tgtcataaga ttacctcttt
    22801 tcttttatta ttaaaataat tgcttacctg agccgtctaa ataattttgt aagatcatca
    22861 cagctgctaa tttatcgatg actttttttc gtttggctct tgaagtattt gcctgctcta
    22921 ctagcatacg ttctgcctga acagtggtta atcgttcatc ttgataatca actggcaagg
    22981 agaatagttc tttgatcttt tctccgtatg acatggaagc ttctgccctt ggtccgatgg
    23041 aattgttcat atttttaggt aatccaacta caaaccggtc tacttcatat tgtgctacta
    23101 attccttcaa ccgatcaaag ccaaactctt cttctgcttc attgatccga atgatctcta
    23161 ctccttgagc tgtccagcca aaggggtcac tgatagcaac acctactgtc ttagaaccga
    23221 catccaagcc cattactctc ataaattcac tccatgattt gagaggtagt ccttgacaat
    23281 tacttccatg atctcatcgc gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg
    23341 aggaatgtag gccgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta
    23401 gcctttttct tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg
    23461 actatcgtca aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt
    23521 tcccgcaagg taattccagc ttaccttcta caaccttatt ttactcgaag aaagggaaaa
    23581 ctacaaacaa atattccaaa cttccgaata aattaagaaa cactatttgt atgttttttt
    23641 ccttttttct gtagctttat caaaagtact cttttcataa agtatacgct atatttcttt
    23701 gaatgtatgt ttttacacaa ttttttcata atattcataa aaaattcgaa tagctgctac
    23761 taattttttg tttttgttgc ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca
    23821 ttgtagataa agacattgaa ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc
    23881 actcccctgg ctaccagttc attcccctca aaatgaggag tgacacggta gcgtacatga
    23941 tgatttgttt ctttgatata tgccgcaata tcattttcgt gagcaagcat gtatggagca
    24001 ttcaacgaac gtgtccctgt catcaagttt ttaatgttgt tattttgacc agtcagctgg
    24061 aaaccaatta aatggctgcg attatataac cattgcccgt cacttaattt cttttgtttc
    24121 caacctgtcg ggtcaatata caaaggctct ctcttttcag tagggaacaa atcttttcca
    24181 agcatagcat tggcttgtcc tactctgttc aatttatcaa tatttgaata gctttcccat
    24241 gttcctttgg aaaggctaag atcctctttt gtaaattccg gctgattccc attgatttct
    24301 atttcttgat gttttccatc atattctggc acatccgcta cttgagaagc ttgtggtgtg
    24361 gaatcaaaaa tagttgtaat ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca
    24421 ccgaatgccc ctgcaatcac agcagcgacg atccccacga tcgccttggt tgtttttttg
    24481 ctcatttctt tactccttta ctaacagctc cctaaatctt aacatatgtt cccctataaa
    24541 taaatactag aaagaaatag aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa
    24601 atacaaaaaa gagacggatt cctcgtctct aatcagtcac cactctatct gtaaggtatt
    24661 tattattatt caaaaatttt cttcatactc aagacaatac actgatttca caaccgcttg
    24721 gtaaacggtg cgcaaaagct tgcatctgcg gtaataagtc catccaagtt aaaaacataa
    24781 gaagttgttt ttgcttggct gttcttatta cttgttgcag agcagctttt tcacaaccta
    24841 ttttaagcgg gtgacgggaa tcgaacccgc gacatcagct tgggaagctg ggattttacc
    24901 actaaactac acccgcaata caaaaacagt atacgcctct tttattcctt cctcaagttt
    24961 ttttattctt tacgcttctg gtatgttttg tagtaaaata aaactaatca atcaattatt
    25021 ggagaagatg ctatgacggg aaaagaatta gatctgttgc tggacacttg tttactcgct
    25081 gggaaaatca tgatggaaag caatgcggag atgtatcggg tagaagatac gatgagtcga
    25141 attgctttag cttctggaaa tttccgtttg gtcagctatg tcactcaaac cggtctcttt
    25201 attggtcttg atcgtacctc gaccatccga atggaacaaa ttaccaaccg ttcgattaat
    25261 ttagaaaaag tagtaaaagt aaatgatctt tcacgtaaat atgttgcttc agagctaaca
    25321 ttagaagaac tttactgcga actgcaagca atcgaccgag accgttcttt ctttcctgtc
    25381 tggttgcaaa tcgttagcgc ggcagtgatc agtggaacaa ttatggtgtt atttggcgga
    25441 aagatgaccg atttacctgt gactttagtc attggaggat tgggatacgc agcttatcta
    25501 tatagtttga aattttttag aatcaaattt ttatcggaat ttttatcttc tttattgatt
    25561 ggcatagccg ctatctacag cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt
    25621 atcggatgtg tgatgccttt agttcctgga gttcaaatta cgaacgctat ccgtgatttg
    25681 ctagctggac attatgtctc aggagtttct cgcggaacag aagcgatgat gactgctacg
    25741 atgatcggtt ttgcgattgc ttttattttc cagctttttt attaggaaag gaacacaagt
    25801 atggggacga tatttattca gttttctttt agttttttag cgactgcggc atttgccgtt
    25861 attacaaatg tacctaggcg ttcattgatt tgttgtggtc tttctggtgc ttttggctgg
    25921 atgatctact ggatctgtgt tcaaataggc ggatctgctg cttttggttc attgcttggt
    25981 tctttaggag tagcattcat cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg
    26041 attttcaata ttcccgggat ggttcctcta gtccctggtg gtttagctta tcaagcggtt
    26101 cgaaatctcg ttactgaatc ctatcgagaa gcggctcatt atgcggtcca agcaattatg
    26161 attgctggag caatcgcttt aggtcttgta ttatcagaag tctttaacca taacatccgt
    26221 aatttcaaag taaagcgtga cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa
    26281 acgaagtgta acaaagaaaa ggttgtgaaa gagacgttca gccttgagcg atgacaaaat
    26341 atctcacaaa cattgcgaca agtaaccgca ggagcaataa gaagtaatta tgagtattaa
    26401 aaaaagtttt gtaaaaatag cttctcacat ttttacataa aattgactta atactgagaa
    26461 actgactttt gaacaccctc tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg
    26521 cccctcttct ttattcaaat tttttccaaa acgttcgttg ataataaacg atccatagag
    26581 cggaaagtat tagaactaaa ccgatcacag gaatattcac taaccaaaat ggataaaaca
    26641 aagccaacat tccgtataag acgataagaa taatactgat gatcaatgaa cccatcatag
    26701 ttgcgactaa acccaaactt cctgaacctc tgttaaataa ttggttaata ttggtccagt
    26761 tcagcagcag caaccgataa tctctagcga aataacgtag tgacaataag tagcagccga
    26821 ataaagaacc aaatagcaag ctagggataa aaaacaaagg cagctgaaaa gacagtccgc
    26881 ttaataatgc gatcccacca gttaaaaaac tctgcaatat ccatccgaat ctgaactttt
    26941 cttttaaata ttggttcata gagattggca aagaacggat aaacagaaaa ttttcttgat
    27001 ccaaagaaat aagattgctg ataaaagaag tctgattaac tgttaagaaa gccaatgcga
    27061 tccctaccag gaaaacaaca ccgattaact tattatctaa atcagttaat tttatttcgc
    27121 ctcctagggc aaaagcaaga ataaaaatga ttggagttaa caaggaagaa gaaagaactt
    27181 gcataatcaa attaggttct tttactaatt gagcattgta actaaataaa agctgatgca
    27241 cattttggtt ctttttatgg gttcttttta ttttgaactc actgtttgaa tcagtcagtt
    27301 gctctgtaag ctttggtact atcagctgtt ttacagcaag gaaaaatccc agagccgtga
    27361 tagctagtat tccccaaccc aataaacctt gttgagaaag tggctgactg gcagcatcat
    27421 agaatggcaa taatatcgaa atcggatgcc gatctgttaa ctcattttca atagtccccg
    27481 tttgatgatt catccacatg attccagcaa atgcgatcag catcgagctg ccaagcaaga
    27541 tactagaaac tgtttttcta tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa
    27601 tcaaactagc cagacaaaaa agaataatca aaaatagaga aaacaaaagg attgagataa
    27661 tgatagtaag caaaatccct agatcagctc gccatccggt taaaataaac atgactagaa
    27721 gcggaaaaac aaaaggaatg atggtcaaag caactaccaa aatctttgct gaaaaaacct
    27781 gttcttgtcc aaaaggcaga ggcaaaaata cttgcaggtc atttccttca aaaaagatat
    27841 tataaatgac tgatatactt tgagttaaac ctaacatacc aaaaagtgcc acgtaataag
    27901 taaagaaacc tggcaattta acaaagttca tagcaaacat agcaaatccg taaatcagta
    27961 aaaaaatcag ccctgaaaaa agattttgag acaaaagata gatagttaat cgctttccct
    28021 tttttccttt ctctcttgct ttattcgtga gctgtggatt aacatataaa agattaactc
    28081 gaattaattc taataattgt cgcttataca tacggtccat ctcctgtcgc ttgccttcca
    28141 gccattttca aataaatctt ttctagcgtt tcatcaggtg agccattcaa taattcacta
    28201 actgccccct gataaatcaa ttcacctttc ttcaaaatag ccagtttatc gcataactgt
    28261 tgtgcggtat ccaatacatg tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc
    28321 ttcatcattt ctttcaaatc aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc
    28381 aatacccaaa tatctggatc tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt
    28441 ccatgtgaaa aacttgcgat tgtttcattt tgatgactat acatatcaaa aagcgctgtc
    28501 aattctgcta aacgcttttc ttttttttgt gtgtctaatt cataggcagc tgaaatcaag
    28561 tcccaatatt ctccagccgt caattgtaaa aaaatatctg gggaatcagg cacataaccg
    28621 attttttgtt tgatactcaa tcgattttcc gtcaatttca ttccatctac taaaatcgtt
    28681 cccgatgatg gttcaataat actaactaaa cttttaatcg tagttgattt tccggcacca
    28741 ttatgtccta aaaatccaaa aatttcccct tgtcttatcg agagagacaa gtcttttaat
    28801 gcttgttttt tcccgtaact tttacttaca ttgatgaatt caatcatttc tgtactcctt
    28861 ttgtttgtta ttacttttta ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag
    28921 tgcataacaa aaaaggatag aaacgactag gctgctactt gggttgttct cttctacagg
    28981 aaaacactct tctaacaggt aagatatgag tctttgtttt ctttaaaatg attcattttc
    29041 tatcttttct tgcataaaat tctttagttt atcaatactt ctctttccca tctctagttg
    29101 ttctccattt gcaaatgtta ctatttcgtt tactctgcta atagatgtaa tgttttcagt
    29161 attaatgata taagctttta aatctttaca taaatactca tattcgagtg attttttata
    29221 ttttttaatt gtgccgttta cgagaatctc ttcatctttc aatactaggc ctattttatt
    29281 tttataacct ggcatgctca ttagataaag gatttcatcg taatggatat agtgatcgct
    29341 attaccataa cggacagttg tccacctttt tttcgtagca tgttgaacag gatagttttc
    29401 tttttctttc aataagtaac tttggatgct ttttttattt ttttcataag tgaattcttt
    29461 tatcaaataa ccactaggcg taatctttcg attaataaca gttattgctt tattcggatg
    29521 agacgttaga taaacaattt tacacttttc attcttttct ctaagcattt cgcctagttc
    29581 gataccgcta taataggcgt tcaaatcgat gtctaggaaa aaaatatctg aggaatagat
    29641 ttcattattt ttaatgaatt caacaaattg tataacgttc aatccctgaa cgatctcaaa
    29701 tttccaagga cttaataaaa ttctggattc ttccaaaatt tctactagca ttttttcaaa
    29761 aaccaaacta tcatccaaaa taaatatacg cgttaccata aaaagtacct ccactcgata
    29821 ttttactatc aaaatcattt catacaactt ttcaacatac atgttaggaa taaattgtaa
    29881 ttctcttcac aatcttttcc aacacctttg aaaccgatat caaatacttt caggatgctg
    29941 cttctaaaat aaatatcaaa caaatgaata catcatgcaa aaaaagccag acaaccgacg
    30001 tccttgcgtg tcagctgtct gagctatgtt tatttatttt gttcttttaa aaagaacgat
    30061 acacatactg atttttcgga gcagagactt tctcagcgtt tgtaacaact acagatggga
    30121 tttcacgctt gaatggttga taatagtctg attggatggt tccttctatt ttataccatt
    30181 catcattttt aaccgttact ccttccggca tatgcgtcaa caaaccaaag actcctgaat
    30241 ctgccacaca atggataatg ccgaatcgga acaagaagat atctgtattg ctatcgttcg
    30301 gtgtttggta aacaaatcct tcatagctga tttttttgcc aatgaattcg ctagggtaat
    30361 tataaattag ctccataact tctaaatagt tttcatctgt gatcttgata gtttgcttat
    30421 ccccatactt ttctttcaac tcattcattt ggtctaagta gtctgatttg ttgaaataaa
    30481 tactggtatc cggctttaag tattgtgtct gcatctccgg atcccctact gattctttac
    30541 ttactgggaa attaaatcct ttggcttcta cgatcgtagt gtcgagacta actgttggaa
    30601 acaacgtccc cacgattact ggcaaagcta gcagtacata agcgattcca cgttgataag
    30661 gttttgacaa cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt
    30721 tgacccacaa atacagctga actaaagcca aaagagcaga taaaatcatt gaaagaatag
    30781 ctaagtagcg ataatggaca ttgatatatt gatcgagttt gccagaaact tgcaagtaca
    30841 tcatcaattc gaaataccca gataaaatca aaaaccgcag catattttcc ctcctaaata
    30901 aataatgtga atccaataac gctaagggca atcagtccga tcagcgataa aataaagcgt
    30961 cctgtaaaat atcgtttcat catcagtaga tttttgatat cgaccattgg gccaaagacc
    31021 aaaaaggcag ctacaggtgc tgtgccgaac agacttaaca aagaagatcc tataaacgca
    31081 tctgcttctg agcaaagaga catcgtaaag gccaataaca gcatgatgat aatggctaaa
    31141 atctttgttg agccgagcgt tagcataatt ctagttggta aataagtctg catcgcagct
    31201 gcgattaatc cgccaatgat caaataacga cctgtatcaa aaaattcgtc gattgaatgt
    31261 gtcaacacat gcccagtttg atggatcagc tgattttttg gttcattctt ctcactttgt
    31321 tcttcttcat ggacatgatg atggtgattt tctgctactt tagacgtgat tttttctttc
    31381 aatacagcgt ctttttgaaa ataggcaagc cagacaccaa tcacaagggc aacaaaaaag
    31441 ctgcctatca cccgccagct tgccatctgc caagaattgc caaatgcgat aaaagtggaa
    31501 aaaatcacga ttggattgat gatcggagca gtcagcataa aagcaaacgc tgtgtgaact
    31561 ggtacgcctt ttttaacaaa ttgatgaacg atcggtacga tgccgcattc acatgaagga
    31621 aagaaaaatc ctaagatcga accagtaaaa atagagaaga tacgattttc aggaagccat
    31681 ctcttcacac gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat
    31741 aatacgaaag gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc
    31801 gaatttggaa taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg
    31861 caaatatacg cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta
    31921 ttttttacca aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat
    31981 acatatcact tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga
    32041 agttcaagac atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg
    32101 cagtgtttgt cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc
    32161 ggcacttcat ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac
    32221 tgcgccagtt gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat
    32281 cgactttaaa aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc
    32341 ttatgaagaa atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca
    32401 agtgaaagtt ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg
    32461 gaatgcgcat ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa
    32521 tcaacatttt ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag
    32581 attcctttga ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt
    32641 atcgttaaaa ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt
    32701 ggataaacgg ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca
    32761 caactgatac gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag
    32821 ttccctacac cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa
    32881 aagtgcataa gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa
    32941 gaaaaatctc gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa
    33001 gacacttttt tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt
    33061 cttttagctg tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc
    33121 taattacatc tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg
    33181 ctcagatatt gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca
    33241 gtataatgga atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt
    33301 cacaaagtag aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt
    33361 ttggtgcaga agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg
    33421 ctttagttgg cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg
    33481 aagcacctga tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc
    33541 aggacacagg acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac
    33601 aagttagaca aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc
    33661 cagacacacc tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt
    33721 cagatcgaaa cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat
    33781 tcgattcttc attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt
    33841 tagctttagc tcgcatgctt ttgcaacacc ctgacatttt gattctagat gaaccaacga
    33901 accatttaga tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg
    33961 cattattgat tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg
    34021 aattaagcag aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga
    34081 aagcagaaca gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata
    34141 aattagaaga cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa
    34201 gccgcagaaa gacattagaa aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac
    34261 aagcaaactt tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag
    34321 atgccgctat cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc
    34381 gtcaagaagc gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat
    34441 caatcattca ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca
    34501 tcggctatta cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat
    34561 tgtgggatga gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct
    34621 tattttctgg cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac
    34681 gggttgcttt agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa
    34741 caaaccattt agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg
    34801 gcacactgct ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg
    34861 tcgaactctc tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga
    34921 aaaagcagga agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc
    34981 ctgctcccac gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta
    35041 gccttcaacg aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg
    35101 atcagttgga acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg
    35161 agctgaacca agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg
    35221 aagaactaag cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc
    35281 attcatgctg ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct
    35341 ttttcaccaa taagaagacc aattactagt cattctcact aaagcttcta aataatagta
    35401 atcaccccaa atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg
    35461 tgttaaaaag ccatcttctt cttccgttgt agcatatctt tctttcaatt gtcgcataat
    35521 tttccaaaca gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat
    35581 taattctaat aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct
    35641 agaatcatca aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa
    35701 ataattggct aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact
    35761 taatgcaaaa ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata
    35821 accttgatgc gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg
    35881 ataagttgaa ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc
    35941 agctttttta tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt
    36001 catgcaacaa tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc
    36061 ttgtataact cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga
    36121 agcttccttc atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata
    36181 caaaaaacca agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc
    36241 tatatctgct ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa
    36301 ccaattaatt cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg
    36361 tccttttata ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa
    36421 cctctccgtg tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc
    36481 tttataacat tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc
    36541 aagcatttcc ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg
    36601 atagttagcc cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa
    36661 ctataatccc gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca
    36721 aatgtaccct ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc
    36781 aaacccataa tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct
    36841 gaaaagccag cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct
    36901 ttctcaggag aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat
    36961 tctactttgt gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt
    37021 gcctgtaaag taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt
    37081 acattatttc gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc
    37141 gtgatatatg tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc
    37201 aattgattcg ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat
    37261 ttttcattgg tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta
    37321 tgtccattat catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga
    37381 ataggcatag gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat
    37441 aaaaaaatct tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc
    37501 aattgagggt aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc
    37561 atccaccatc ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa
    37621 agatcggccc acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt
    37681 cgaccaaaag gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa
    37741 tcaatagctc ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa
    37801 atcaaaccat aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg
    37861 tctcgatacc agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt
    37921 gctgattctt taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa
    37981 aattgccaat taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc
    38041 tggctttttt ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct
    38101 aataacattg cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa
    38161 tcgggatggg aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt
    38221 tcatctaatt ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt
    38281 cctgcaattt tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt
    38341 gctcgataaa acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt
    38401 ctggtcctta atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa
    38461 tagctaattc cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga
    38521 tcagtggcac attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc
    38581 catcttcatc catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag
    38641 cagcttcatg atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac
    38701 cgcatgcagc gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa
    38761 tgagaggaat ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg
    38821 ctatagctag atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag
    38881 cccatgcatt tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc
    38941 gaatatattt ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc
    39001 tcactagata cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat
    39061 caccagtttc cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca
    39121 tccaaccagg tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc
    39181 cagcaagtac cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt
    39241 gataatgtgc aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa
    39301 aatggtacag ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt
    39361 ctcgttttgt cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc
    39421 agtcgattcc tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa
    39481 ttttattccc cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt
    39541 ttcttgacca ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat
    39601 gaatgtggag ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta
    39661 tttgcagctc caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc
    39721 attgggatca ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct
    39781 gtactagaaa ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa
    39841 agcatgacta atggaaatag atattgattc caacttccca taaagataag taaaccacta
    39901 gtaataatcg taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg
    39961 gcaccatcga ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc
    40021 atcataaaca ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc
    40081 gataatccta aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc
    40141 atcctagata gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct
    40201 aatgcataac ccgccattgt agaactggct gtagctaaaa ctgtactgat tacagcaata
    40261 acaatcgaat tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt
    40321 tttaatagca tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt
    40381 tgcgttgaag ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca
    40441 ataatgacgt acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc
    40501 gaatttaaat tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc
    40561 agaggctttt ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac
    40621 caaagctgaa ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt
    40681 acccaagcca ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg
    40741 gatcgttaat aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta
    40801 tatctcttta gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact
    40861 aagccaaatc atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg
    40921 tacaccgtta attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa
    40981 agtcatgaat acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt
    41041 tctaaaaaat ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc
    41101 taatagcatc gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt
    41161 aataaacaat ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga
    41221 gtagccaatt ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac
    41281 aataaagaaa ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc
    41341 tgttgctttt ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt
    41401 taagtcctcc taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat
    41461 tttctgcttc ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag
    41521 cggtagtcaa ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat
    41581 tttcattgat tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt
    41641 tctcagctgc ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg
    41701 cgttggctaa gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg
    41761 gatttttggc atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg
    41821 gattttcttt tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa
    41881 aattcaaaga catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa
    41941 atgctttctc agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt
    42001 tgattaactt tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat
    42061 caagcaaaga ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt
    42121 ctggcaaggc aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat
    42181 attcttccca agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt
    42241 gataataaca aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg
    42301 tttgttgacg caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa
    42361 atccatattc atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg
    42421 aagtgtctgc catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga
    42481 tcatttgtac tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc
    42541 cagttttgcg gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg
    42601 cgacatatac ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta
    42661 cttcttcgtt agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag
    42721 ctatcaccaa tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata
    42781 taaaacgctt tcacttatgt atattaacgg aattagtaac acaaatattt atataaaaaa
    42841 atattttact tgtcgttttg gcttttcatt tttgttataa tagtcattaa ttataaatag
    42901 aaagcttttt atataaaaag agaaaggaaa gtggttcttt tgttaacaaa caaccaatcc
    42961 tccaatcttg tcaaaaaaac actaacctat tccatagata cccatcttca tttatctgct
    43021 gctccctatt ttcgactact aataaatgaa agcgctaatt tatatgctga atttgcacat
    43081 aaatctatag aactagataa tccttccctt ttattcattg ataaacagca agaagtccaa
    43141 atcttttcta ctcaaaagga aacacaaact tttatcatta tccagtttta ttttgatgag
    43201 caattattag cacatattac gggacaatct ttttctatag atcaagaaat aacatctatc
    43261 attacttttc tgaaaaatga acagttcaaa attgattatc taaaacatag taagccagct
    43321 atttcctatg atcaggaaat caattctttt ttcatactat gccaacagat gagcattcat
    43381 ttgctttcct tattagttac ttattgtctg caaaaaattc aattgtcttc cttaccaagg
    43441 cattatcaac aggaacttgc tttattgaca ataccttcaa atgagaaagt cattcctaaa
    43501 aaaaagcata ttctttattc agatgaatta gttgatccaa tcatacgata tttagaagaa
    43561 catctttcag aaaatttttt gcttgaacaa ttagcccaaa ttttttttat cagtccagcc
    43621 agcttaaaaa aacattttag aaaagtgaat ggaaaaagtg ttattagcta ttttaaagaa
    43681 ctaaaaatgg aaaaagcaaa acgtctcgtg ctagaaaatg aattaaccta tacagaaatt
    43741 gcagatatat taggatttca ttcgatccat catttttctt cagcttttaa aaaatataca
    43801 gggttatccc cttcaaagta ttatctatcc ttaaaataga aaaaaatttt taggttttat
    43861 ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt ttccggcaca
    43921 attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta
    43981 tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc
    44041 gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa
    44101 gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac
    44161 gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa
    44221 cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa
    44281 taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata
    44341 ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc
    44401 gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg
    44461 ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg
    44521 atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca
    44581 aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata
    44641 aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg
    44701 ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata
    44761 caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg
    44821 gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca
    44881 gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct
    44941 atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg
    45001 gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca
    45061 ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt
    45121 ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata
    45181 aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg
    45241 atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg
    45301 gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac
    45361 ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt
    45421 ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg
    45481 aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt
    45541 ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg
    45601 accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag
    45661 tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa gaaattgtgc
    45721 attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg cgcaatttaa
    45781 ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc
    45841 catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat taatctatca
    45901 gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct
    45961 ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt
    46021 ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc
    46081 cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat
    46141 ttaa
//
LOCUS       DAZBTH010000011        44939 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000011 DAZBTH010000000
VERSION     DAZBTH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.011"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHGNW_000616"
     CDS             complement(38..1189)
                     /locus_tag="ACHGNW_000616"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN6924958.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHGNW_000617"
     CDS             complement(1202..1639)
                     /locus_tag="ACHGNW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6924959.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHGNW_000618"
     CDS             complement(1654..3420)
                     /locus_tag="ACHGNW_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN6924960.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHGNW_000619"
     CDS             3684..4361
                     /locus_tag="ACHGNW_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6924961.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHGNW_000620"
     CDS             4364..5491
                     /locus_tag="ACHGNW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6924962.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHGNW_000621"
     CDS             complement(5691..6131)
                     /locus_tag="ACHGNW_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6924963.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHGNW_000622"
     CDS             complement(6142..8175)
                     /locus_tag="ACHGNW_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN6924964.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHGNW_000623"
     CDS             complement(8177..9880)
                     /locus_tag="ACHGNW_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6924965.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHGNW_000624"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHGNW_000624"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN6924966.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHGNW_000625"
     CDS             complement(10963..11988)
                     /locus_tag="ACHGNW_000625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6924967.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHGNW_000626"
     CDS             complement(12197..13066)
                     /locus_tag="ACHGNW_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6924968.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHGNW_000627"
     CDS             complement(13525..15417)
                     /locus_tag="ACHGNW_000627"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924969.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHGNW_000628"
     CDS             complement(15414..17141)
                     /locus_tag="ACHGNW_000628"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6924970.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHGNW_000629"
     CDS             complement(17157..17807)
                     /locus_tag="ACHGNW_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN6924971.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHGNW_000630"
     CDS             18011..18307
                     /locus_tag="ACHGNW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6924972.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHGNW_000631"
     CDS             18479..19099
                     /locus_tag="ACHGNW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6924973.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHGNW_000632"
     CDS             complement(19191..20357)
                     /locus_tag="ACHGNW_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6924974.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHGNW_000633"
     CDS             complement(20369..20722)
                     /locus_tag="ACHGNW_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6924975.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHGNW_000634"
     CDS             20850..21872
                     /locus_tag="ACHGNW_000634"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN6924976.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHGNW_000635"
     CDS             21891..22052
                     /locus_tag="ACHGNW_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924977.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHGNW_000636"
     CDS             complement(22311..22745)
                     /locus_tag="ACHGNW_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6924978.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHGNW_000637"
     CDS             complement(22855..24714)
                     /locus_tag="ACHGNW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6924979.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHGNW_000638"
     CDS             24715..24855
                     /locus_tag="ACHGNW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN6924980.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHGNW_000639"
     CDS             complement(24832..25992)
                     /locus_tag="ACHGNW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6924981.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHGNW_000640"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHGNW_000640"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN6924982.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHGNW_000641"
     CDS             complement(27739..28437)
                     /locus_tag="ACHGNW_000641"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6924983.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHGNW_000642"
     CDS             complement(28394..30055)
                     /locus_tag="ACHGNW_000642"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN6924984.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHGNW_000643"
     CDS             30273..31673
                     /locus_tag="ACHGNW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN6924985.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHGNW_000644"
     CDS             complement(31925..33004)
                     /locus_tag="ACHGNW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6924986.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHGNW_000645"
     CDS             complement(33090..34049)
                     /locus_tag="ACHGNW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN6924987.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHGNW_000646"
     CDS             complement(34061..35572)
                     /locus_tag="ACHGNW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN6924988.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHGNW_000647"
     CDS             complement(35594..36157)
                     /locus_tag="ACHGNW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6924989.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHGNW_000648"
     CDS             complement(36163..37026)
                     /locus_tag="ACHGNW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924990.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHGNW_000649"
     CDS             complement(37040..37936)
                     /locus_tag="ACHGNW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6924991.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHGNW_000650"
     CDS             complement(38111..39400)
                     /locus_tag="ACHGNW_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6924992.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHGNW_000651"
     CDS             complement(39372..40889)
                     /locus_tag="ACHGNW_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN6924993.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHGNW_000652"
     CDS             41087..41977
                     /locus_tag="ACHGNW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN6924994.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHGNW_000653"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHGNW_000653"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN6924995.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHGNW_000654"
     CDS             complement(42886..44358)
                     /locus_tag="ACHGNW_000654"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN6924996.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DAZBTH010000012        43698 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000012 DAZBTH010000000
VERSION     DAZBTH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.012"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(338..2719)
                     /locus_tag="ACHGNW_000655"
     CDS             complement(338..2719)
                     /locus_tag="ACHGNW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6924997.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2709..3356)
                     /gene="recU"
                     /locus_tag="ACHGNW_000656"
     CDS             complement(2709..3356)
                     /gene="recU"
                     /locus_tag="ACHGNW_000656"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN6924998.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3420..3959
                     /locus_tag="ACHGNW_000657"
     CDS             3420..3959
                     /locus_tag="ACHGNW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN6924999.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4049..4468
                     /gene="gpsB"
                     /locus_tag="ACHGNW_000658"
     CDS             4049..4468
                     /gene="gpsB"
                     /locus_tag="ACHGNW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN6925000.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4490..4857
                     /gene="rnpB"
                     /locus_tag="ACHGNW_000659"
     ncRNA           4490..4857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHGNW_000659"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5066..6232
                     /locus_tag="ACHGNW_000660"
     CDS             5066..6232
                     /locus_tag="ACHGNW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN6925001.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6278..7774
                     /locus_tag="ACHGNW_000661"
     CDS             6278..7774
                     /locus_tag="ACHGNW_000661"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN6925002.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7855..8538
                     /locus_tag="ACHGNW_000662"
     CDS             7855..8538
                     /locus_tag="ACHGNW_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6925003.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9183..10373
                     /locus_tag="ACHGNW_000663"
     CDS             9183..10373
                     /locus_tag="ACHGNW_000663"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN6925004.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10475..11428
                     /locus_tag="ACHGNW_000664"
     CDS             10475..11428
                     /locus_tag="ACHGNW_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN6925005.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11497..12846
                     /locus_tag="ACHGNW_000665"
     CDS             11497..12846
                     /locus_tag="ACHGNW_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6925006.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13029..13352)
                     /locus_tag="ACHGNW_000666"
     CDS             complement(13029..13352)
                     /locus_tag="ACHGNW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925007.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13481..14644
                     /locus_tag="ACHGNW_000667"
     CDS             13481..14644
                     /locus_tag="ACHGNW_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6925008.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14714..16063
                     /locus_tag="ACHGNW_000668"
     CDS             14714..16063
                     /locus_tag="ACHGNW_000668"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6925009.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16108..16539)
                     /locus_tag="ACHGNW_000669"
     CDS             complement(16108..16539)
                     /locus_tag="ACHGNW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925010.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16927..19569
                     /gene="alaS"
                     /locus_tag="ACHGNW_000670"
     CDS             16927..19569
                     /gene="alaS"
                     /locus_tag="ACHGNW_000670"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN6925011.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19769..20365
                     /locus_tag="ACHGNW_000671"
     CDS             19769..20365
                     /locus_tag="ACHGNW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6925012.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20487..21635
                     /locus_tag="ACHGNW_000672"
     CDS             20487..21635
                     /locus_tag="ACHGNW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6925013.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21673..22086)
                     /locus_tag="ACHGNW_000673"
     CDS             complement(21673..22086)
                     /locus_tag="ACHGNW_000673"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN6925014.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22165..22596
                     /locus_tag="ACHGNW_000674"
     CDS             22165..22596
                     /locus_tag="ACHGNW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN6925015.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22695..22928)
                     /locus_tag="ACHGNW_000675"
     CDS             complement(22695..22928)
                     /locus_tag="ACHGNW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6925016.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23111..24781
                     /locus_tag="ACHGNW_000676"
     CDS             23111..24781
                     /locus_tag="ACHGNW_000676"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN6925017.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24928..25314
                     /locus_tag="ACHGNW_000677"
     CDS             24928..25314
                     /locus_tag="ACHGNW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925018.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25301..25999
                     /locus_tag="ACHGNW_000678"
     CDS             25301..25999
                     /locus_tag="ACHGNW_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN6925019.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26004..26468
                     /gene="lspA"
                     /locus_tag="ACHGNW_000679"
     CDS             26004..26468
                     /gene="lspA"
                     /locus_tag="ACHGNW_000679"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN6925020.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26465..27376
                     /locus_tag="ACHGNW_000680"
     CDS             26465..27376
                     /locus_tag="ACHGNW_000680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6925021.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27611..28150
                     /gene="pyrR"
                     /locus_tag="ACHGNW_000681"
     CDS             27611..28150
                     /gene="pyrR"
                     /locus_tag="ACHGNW_000681"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN6925022.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28164..29447
                     /locus_tag="ACHGNW_000682"
     CDS             28164..29447
                     /locus_tag="ACHGNW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN6925023.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29460..30386
                     /locus_tag="ACHGNW_000683"
     CDS             29460..30386
                     /locus_tag="ACHGNW_000683"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN6925024.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30405..31685
                     /locus_tag="ACHGNW_000684"
     CDS             30405..31685
                     /locus_tag="ACHGNW_000684"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN6925025.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31690..32766
                     /locus_tag="ACHGNW_000685"
     CDS             31690..32766
                     /locus_tag="ACHGNW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN6925026.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32771..35956
                     /gene="carB"
                     /locus_tag="ACHGNW_000686"
     CDS             32771..35956
                     /gene="carB"
                     /locus_tag="ACHGNW_000686"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN6925027.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36010..36786
                     /locus_tag="ACHGNW_000687"
     CDS             36010..36786
                     /locus_tag="ACHGNW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN6925028.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36783..37715
                     /locus_tag="ACHGNW_000688"
     CDS             36783..37715
                     /locus_tag="ACHGNW_000688"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN6925029.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37712..38428
                     /gene="pyrF"
                     /locus_tag="ACHGNW_000689"
     CDS             37712..38428
                     /gene="pyrF"
                     /locus_tag="ACHGNW_000689"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN6925030.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38435..39061
                     /gene="pyrE"
                     /locus_tag="ACHGNW_000690"
                     /pseudo
     CDS             38435..39061
                     /gene="pyrE"
                     /locus_tag="ACHGNW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            39088..39366
                     /locus_tag="ACHGNW_000691"
     CDS             39088..39366
                     /locus_tag="ACHGNW_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925031.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39404..40045
                     /locus_tag="ACHGNW_000692"
     CDS             39404..40045
                     /locus_tag="ACHGNW_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN6925032.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40088..40780
                     /locus_tag="ACHGNW_000693"
     CDS             40088..40780
                     /locus_tag="ACHGNW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN6925033.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40777..41679
                     /locus_tag="ACHGNW_000694"
     CDS             40777..41679
                     /locus_tag="ACHGNW_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6925034.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41837..43543)
                     /gene="efbA"
                     /locus_tag="ACHGNW_000695"
     CDS             complement(41837..43543)
                     /gene="efbA"
                     /locus_tag="ACHGNW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN6925035.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14002 a         7700 c         9490 g        12506 t
ORIGIN      
        1 ctcaaaatga agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg
       61 ataataacaa tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc
      121 gatttaatta taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt
      181 ttctaatttt taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa
      241 atcatgtaat acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa
      301 aaaagaaacc acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg
      361 tgctgctgga tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg
      421 aagaagcagg aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa
      481 ccgttgttga agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag
      541 cagttgattc gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc
      601 ttccaggagt ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg
      661 atctagctgt atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg
      721 gcatctccca gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa
      781 catcagaggc tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg
      841 tccacacgga aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc
      901 cagaagaaat accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac
      961 cggcttgata taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt
     1021 ttaaaatgtc tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct
     1081 cataagtttc ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat
     1141 aagtccctcc attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt
     1201 attttgtccc atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac
     1261 tgtattcgat cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga
     1321 ataatttgac tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc
     1381 ccatatattg attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg
     1441 cgtcagtaat agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg
     1501 tgacaggttt catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta
     1561 aagttacgtc ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg
     1621 tgtcgataag ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg
     1681 tgttgacgat atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta
     1741 aaccgtctgt gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga
     1801 cataattatc gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg
     1861 ttaccggtac attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca
     1921 tcgtatacaa aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat
     1981 aggcggaagg ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg
     2041 ataatttctt accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca
     2101 tatacacttt atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc
     2161 ttacagccat ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg
     2221 agaagaagga aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag
     2281 aggtaaagtt agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat
     2341 aaaaacggcg atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg
     2401 cagaaatttt ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat
     2461 atagtttgga agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg
     2521 cgtaatacca gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc
     2581 ctaaaaggat cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt
     2641 tgccagaatt cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc
     2701 tagtttgttc atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag
     2761 tcaaccgcgt ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt
     2821 tcttcgatca aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa
     2881 gcaatcaaat gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag
     2941 acaaagcaga ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg
     3001 aagtttttaa agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa
     3061 aagcctcgat agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg
     3121 atcacagcag cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt
     3181 ttatggataa ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct
     3241 tcaaagcgca tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt
     3301 tcgacgggag aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt
     3361 aatcacactc ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga
     3421 tggataagtc aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta
     3481 aagaaaatga tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt
     3541 tagcagaaga agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg
     3601 ctgtgcaaat agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt
     3661 atcctttttc tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag
     3721 cagaacaatt agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc
     3781 agctaaagaa tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg
     3841 atgaagaatt ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag
     3901 atcatcccta cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat
     3961 cgatattgtg tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat
     4021 tacgtaaatg aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat
     4081 tttacaacaa gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt
     4141 cttagacaat atcatcaaag attatgaagc ttacagcaaa gaactccttg ctttacaaga
     4201 agaaaacgat cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca
     4261 aacacgcgtg caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt
     4321 aaaacgcttg tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag
     4381 tgcagtcaaa ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga
     4441 tgataatgaa aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta
     4501 atcgcggtct gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg
     4561 tagtgttcgt gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa
     4621 caagctaagg cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct
     4681 ggggaaaccc atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta
     4741 ggggcgcttt ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg
     4801 gttaccgctt tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac
     4861 cgcaaacatc agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac
     4921 atttgaaagc tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg
     4981 tatcaccggt tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct
     5041 ttttttataa ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac
     5101 tgcggcaagc gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg
     5161 ccaagttgag aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa
     5221 tttatggtta cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac
     5281 gtttgatgaa ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga
     5341 tgcagctttt cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg
     5401 ccaagcgatc acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc
     5461 ttctgtacca ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga
     5521 ccatgtgatg gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt
     5581 agaaaaaggt ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa
     5641 ttggcgaaaa gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga
     5701 agcagcctta ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc
     5761 ttgggattgg ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc
     5821 agattatgat attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat
     5881 tgccaaagcc aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc
     5941 agtcaaagac ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg
     6001 agaacgtcta ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt
     6061 ccgtccatta aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt
     6121 ttacggacaa aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct
     6181 attccagtat tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta
     6241 attgattcat tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca
     6301 agaagtaaaa gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca
     6361 aacaggtatg cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag
     6421 catttatttt tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga
     6481 acatccggaa gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta
     6541 tgatttggaa catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc
     6601 agcacacgga caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc
     6661 attgaatcaa aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa
     6721 gacgaattat gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga
     6781 taaggtattt gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa
     6841 agggactgaa cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag
     6901 atttgtagtg aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga
     6961 cactgtccac ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg
     7021 gaacgaaaca gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat
     7081 gtatgaacaa gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat
     7141 gggtatccat gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt
     7201 ctggaaaaag caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc
     7261 ttttgacaaa ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc
     7321 agacagtttg acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt
     7381 caatgaaggg gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta
     7441 tctgggaata cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg
     7501 tgcaagcttt ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact
     7561 tcattctttg cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc
     7621 aatcaaaaac tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca
     7681 attaatcatg gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa
     7741 agacctgtat tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa
     7801 ttcatctaat ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat
     7861 aaattacaag tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat
     7921 gggagcgatc ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg
     7981 caggcagacc gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa
     8041 gtcgttaaaa atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca
     8101 atagtagagg aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa
     8161 aatctctctg aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt
     8221 atcgaagcat taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg
     8281 cacccgattt ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga
     8341 aaaggcacga cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg
     8401 aatactgatt acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta
     8461 gaagaatttt ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc
     8521 aaaaataaca aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga
     8581 tcattttccg taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt
     8641 tcagctccga gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg
     8701 taacttgtga ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac
     8761 aagacttttg tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca
     8821 gagagtaaaa ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt
     8881 ctatgcaata aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat
     8941 caatggttgt gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt
     9001 aattgttcat actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg
     9061 ttaaataatt ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag
     9121 atgaatgaca cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga
     9181 caatgtcttc caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa
     9241 aggggttata caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag
     9301 acggaaaaaa gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag
     9361 aattgaaaaa agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc
     9421 gaacgatcaa tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca
     9481 aagggacaga agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct
     9541 ctgcagtaat gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta
     9601 ttgacggttg ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag
     9661 accttgagaa aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata
     9721 tcacagatgg tgttttctcc atggatggag acattgcacg attgccggaa attattccga
     9781 ttgttgaaaa atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg
     9841 gaaaaggtgc aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg
     9901 gcacgttatc gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa
     9961 tcgactggtt gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg
    10021 cagctgcagc tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa
    10081 aagtttggga aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg
    10141 gtatgtctga aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt
    10201 tctctaagaa gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc
    10261 ctcttgggac aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg
    10321 atgaagcagt agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt
    10381 tagttttttc gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg
    10441 aagatagtag taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg
    10501 tttaggacaa attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa
    10561 tgtcattgcg acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat
    10621 cctagatgta atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac
    10681 attgattcat ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg
    10741 gaatttgaac gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa
    10801 attttttacg ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc
    10861 acaagatact cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact
    10921 actatgtgat tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg
    10981 attgatttct tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta
    11041 ttattcagca atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga
    11101 tatgatgtat atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga
    11161 tcgattggtt catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat
    11221 caaagcatct attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt
    11281 acgtcagtca atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga
    11341 atggggcttt tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact
    11401 gaaagaaaaa ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac
    11461 ttttgggatt ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca
    11521 gtaaaagaag taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta
    11581 tcgactgtta tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca
    11641 gaagttacag gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct
    11701 gtctgtgcag gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg
    11761 attaaatata tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa
    11821 gtcgtgattt attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc
    11881 gtcaatctat ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta
    11941 tcgatcctgt tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg
    12001 cttgtctgca agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa
    12061 ggagtagact tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc
    12121 ttgggagcgg gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat
    12181 atttcgggag aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc
    12241 atcggaatca tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc
    12301 gacggagtcg caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc
    12361 ttcggtggaa gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc
    12421 tataccctta ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt
    12481 agcaagctct ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta
    12541 gagctagtca tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg
    12601 ctgatctttg tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt
    12661 aaaaaagaac ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta
    12721 gtagcaatca ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta
    12781 gcttcgatgg ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt
    12841 agatagctaa aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt
    12901 atttaaatga ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa
    12961 atgtcgtttt gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt
    13021 tctgtctttt actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt
    13081 aataaaacca agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca
    13141 agcactttag acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa
    13201 gtctgaagat tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa
    13261 tgaattgtag ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata
    13321 ggtacgccaa aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac
    13381 tttactttga aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact
    13441 gctaagataa gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc
    13501 gattcgtttt gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt
    13561 tatttatgaa tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc
    13621 tttcgttttt tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga
    13681 gacggttgcc caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca
    13741 tttcagagac aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa
    13801 gcagagtgta tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta
    13861 tttaagatat tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc
    13921 tctatttttt gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg
    13981 cttatttgat gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt
    14041 tacgccgtta tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga
    14101 aaaaggaaag actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt
    14161 tttatctctt attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc
    14221 agttacttct catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc
    14281 gtttataacc aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga
    14341 agaccgcgaa cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct
    14401 tttttatggt ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa
    14461 tacggtgttg cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat
    14521 ttttatttta aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa
    14581 agctagtcac acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa
    14641 ttagctgagc aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag
    14701 aagaggtgca cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc
    14761 gttaaccgca aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat
    14821 tgcaaaagga aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt
    14881 tttgttgcct ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac
    14941 agctccaagt agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt
    15001 ttctacaccg gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt
    15061 aaatcgtctg catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga
    15121 catgatgaat gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc
    15181 ggatatgaca ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc
    15241 agaaaagctg cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt
    15301 acgaaaatac atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga
    15361 agacattgaa aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca
    15421 actgctgaca attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt
    15481 ggatgaaatt gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga
    15541 catcacccca agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata
    15601 tgttgtagcg actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat
    15661 taatgcagaa attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg
    15721 taatggctta aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga
    15781 tgcgatagaa aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt
    15841 caaaacatac gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc
    15901 cacgctgatt ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa
    15961 aatcgattgg gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca
    16021 aagccgtact gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga
    16081 caaaaggcgt gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag
    16141 tacttcatct tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc
    16201 tacagccgtc atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt
    16261 ttcagcagct tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca
    16321 aaatcgttcc ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg
    16381 tcttttttga tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc
    16441 tagttctgca ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc
    16501 tgcgcgtatc cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa
    16561 tagcagagat gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa
    16621 aaggaaatga aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt
    16681 aagtacgaaa aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa
    16741 aaaagaatag tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca
    16801 gctcagcgtt atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc
    16861 gcgagttttc gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt
    16921 aatgacatga aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa
    16981 tctaaaggac atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta
    17041 ttgtggatca actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca
    17101 gaaaatccaa gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc
    17161 ggtaaaacag cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac
    17221 tatttcaaaa atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg
    17281 gcattcgatc cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc
    17341 atctggcatg aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc
    17401 tgggatatcg gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa
    17461 gcttataacg atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt
    17521 tatttagaaa tctggaactt agtattttca gaattcaacc ataagcctga taacacattt
    17581 gaaccacttc ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc
    17641 gttcaagatg cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta
    17701 gaaaagctaa gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa
    17761 gtaatcgcag atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg
    17821 aatgaaggga gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa
    17881 aaattaggta ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg
    17941 gtaagttatt atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca
    18001 gaagaagaac gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc
    18061 caagaagtaa aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac
    18121 gacacatatg gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa
    18181 gttgatcatg ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc
    18241 cgcagtactg aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa
    18301 agtaaattca ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa
    18361 ggtgagtctc ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca
    18421 cctttctatg cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta
    18481 ggcgaaatcg tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac
    18541 accgtagaag taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa
    18601 tcgatgcgca atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg
    18661 aaagaagtac ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta
    18721 cgattcgact ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga
    18781 atcgtcaacg aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat
    18841 acagccaaaa acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga
    18901 gtagtaaata ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca
    18961 gaaaacatcg gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga
    19021 atcgaggctg taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta
    19081 aaacaagtag caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa
    19141 caattgcaac agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta
    19201 gcgaatcaac aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc
    19261 attgcagcac aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg
    19321 aaacaaaaaa atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt
    19381 ctgctagcgg caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt
    19441 aaagcaatcg caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct
    19501 ggcgggaaaa atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg
    19561 caacaatagg gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg
    19621 attatggggg cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat
    19681 ctaatcaaat ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg
    19741 attagtataa ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt
    19801 cttacgtgcg atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt
    19861 tattccatta ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt
    19921 agtattaggc ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt
    19981 tcgtgcctat gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat
    20041 ctcggtagtt cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta
    20101 tcaaaaaaat aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt
    20161 caatacagtc ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc
    20221 ctatggtgta gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg
    20281 gataccagaa gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc
    20341 agcaacaccg ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag
    20401 atagaaaaag gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa
    20461 caagcaaact agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa
    20521 taccgcaaca gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc
    20581 tgatcttgtt tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg
    20641 ctgatgcagt gaacagtcta tcagatactg cttcatccat attgactttg atcggattta
    20701 aaattgctgc aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata
    20761 tcagtgggtt atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt
    20821 ctgctgaaaa aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc
    20881 taatcatttc catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat
    20941 ccattgattc tgaaacattg agagctacag caacagatag ttataatgat gtctttacta
    21001 cactggctgt attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat
    21061 atataggttt tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt
    21121 ttgtatacga actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc
    21181 atttaaatgc ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac
    21241 ccaataaacg ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg
    21301 ctcataagat tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg
    21361 tctgtcactt ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt
    21421 taaagcaaat tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc
    21481 atcaaggaaa aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata
    21541 cagaactaga aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg
    21601 aaattacgtt tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt
    21661 aaagttttta atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc
    21721 aagattatct gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa
    21781 ttcggataga agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc
    21841 gtttttgtcg accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt
    21901 ctgtaagtcg tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg
    21961 atggtttgtc cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat
    22021 caagattcca cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt
    22081 taacataaaa agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt
    22141 actgaatcgg tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca
    22201 catacgatca tctattggtc attgaccttg atcattctgt tttttagtct gattctttct
    22261 ttagaaaaca caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt
    22321 ctatttctag gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat
    22381 gctgcttttt ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact
    22441 aatactgggg tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga
    22501 aaaaaagatc aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca
    22561 attccttatc cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa
    22621 acgaattttt acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca
    22681 ctctatttgt ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg
    22741 acatgggcag cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt
    22801 tcttccaaag ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct
    22861 gtgtcatcat aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct
    22921 gtggtcaaag cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat
    22981 ttataagtac gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa
    23041 aaggtataat tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag
    23101 gggtaatcaa atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt
    23161 tgaagtggct agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa
    23221 ggcgaaaatc aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat
    23281 cttagttact tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg
    23341 gttaggagac gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc
    23401 acttggtcct gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt
    23461 gcctatggaa gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa
    23521 taatgcatta tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga
    23581 tgcccgccga gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt
    23641 aattgttggc ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac
    23701 agttgccagc gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc
    23761 tcgtcttgca aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga
    23821 cttgaaagca gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt
    23881 acaaacaatt tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca
    23941 tggctgcaat agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac
    24001 agaggcagga tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa
    24061 cttgaaaaaa gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca
    24121 tggcggaatg aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt
    24181 tgcaaattta aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc
    24241 aatcaatgaa ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga
    24301 aaatcagggg attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt
    24361 tgatttagca aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt
    24421 ataccgccta gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg
    24481 aggaaatggt gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa
    24541 tggatgggac aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc
    24601 ttcactgcta ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt
    24661 aggggcagga tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa
    24721 aaaacctgct gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta
    24781 agcttgatta taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga
    24841 cgatagggaa ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc
    24901 tatactaaag cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt
    24961 tacggtatgg gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt
    25021 catgaacaaa agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat
    25081 tggctaaaaa gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg
    25141 aattacttga ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt
    25201 ttcctacaaa atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc
    25261 tatataaaaa accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt
    25321 tttaatcagt ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa
    25381 tatgggattt accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa
    25441 atacgaagat gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat
    25501 cgcagtcgat tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat
    25561 cgaacaagaa ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg
    25621 ttttgaacaa gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc
    25681 acaatttccg ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg
    25741 gttttggcta gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt
    25801 ggatttgatc atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca
    25861 tatttacgag gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac
    25921 taaagatggg aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa
    25981 acaaatagaa aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag
    26041 taggagtgga tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga
    26101 aaaatcttat accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga
    26161 gtatgctaga agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga
    26221 ttctcacatt gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga
    26281 gtttgattct agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag
    26341 tagatatgtt ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac
    26401 ttgtatgtgg agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa
    26461 aaaaatgaca gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt
    26521 gactaattta ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc
    26581 ggtttctgtc aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat
    26641 tactgtcttg atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac
    26701 gatcgtttac gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca
    26761 tccttcagca ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga
    26821 tctttctacg atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac
    26881 ttccggactg ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt
    26941 aaaagataag acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga
    27001 aaaaggaacc atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat
    27061 aagagaagat gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt
    27121 tactctggtt gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa
    27181 gtatatcggt tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg
    27241 caatggtcag tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa
    27301 gatgatcttt gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa
    27361 aaacattgca ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta
    27421 atagaattcc tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat
    27481 ggtgcgccat aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc
    27541 tcagtctaaa cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg
    27601 gggaatgaaa atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac
    27661 tcgaattact tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg
    27721 tatcaaaaca cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga
    27781 agatatcgat attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga
    27841 aaatccagaa gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga
    27901 agttattttg attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc
    27961 agtaatggat tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca
    28021 tcgagaattg ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga
    28081 agagatttta gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga
    28141 ggaagattag aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg
    28201 tgttagatat ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact
    28261 tgttcacgat gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta
    28321 ttgcgctagt cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga
    28381 tacctgcgta cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa
    28441 cagatggtta tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg
    28501 ttgcgctgat catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg
    28561 tcgtcggtcc agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg
    28621 ctatgtataa tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag
    28681 gattaacaat tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac
    28741 taggtattat tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa
    28801 tcgtagacgc tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac
    28861 caaagctata tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag
    28921 agcatatcgg ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac
    28981 cgggtcttca taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg
    29041 gaggtcctcc tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc
    29101 atagcgtgtt tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa
    29161 aattaagcgc tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct
    29221 tatttggagt aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg
    29281 ataaaaagaa aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg
    29341 tctttgaagt aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca
    29401 ttttaaatct tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta
    29461 tgatcatcaa atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg
    29521 atcaagaagt tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga
    29581 ctccgcaaaa gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac
    29641 ataaaagctt cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa
    29701 gcacaagctc tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat
    29761 taggcgtaga cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac
    29821 aaagtaaaac aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa
    29881 cccaatgctt gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga
    29941 aagttgccat cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt
    30001 taaaacgtct aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat
    30061 tcgaagtgta cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga
    30121 tgatgcttcg ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct
    30181 accatcagga acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca
    30241 ttatgcaccc agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat
    30301 tacagtcgcg gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag
    30361 aagcagtgct aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta
    30421 aaaacggtca gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag
    30481 atgggcgaat caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg
    30541 atgcaaaggg acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc
    30601 caggatttac atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt
    30661 tcactactgt atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga
    30721 gacaagtata tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa
    30781 tcacagaaaa tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg
    30841 cttttgcttt cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta
    30901 tgaaagaagc tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat
    30961 tatttggcgg cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct
    31021 taagcgtaac cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg
    31081 ttcattacca tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa
    31141 aaaaagcagg aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg
    31201 aagatattcc cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg
    31261 acagagaagc tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc
    31321 atgcccccca tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg
    31381 taggaagcga aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca
    31441 cgttggaaca agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg
    31501 caggaacgct aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt
    31561 gcacgatcga taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga
    31621 aagtaaaagg cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag
    31681 aataaaagta tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct
    31741 tttggtgcag atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat
    31801 caagaaacta tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg
    31861 gtgggtaatt atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga
    31921 gtagtagtaa aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat
    31981 tcatttttga aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact
    32041 cgcaaaatcc gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc
    32101 gaacatgcat ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc
    32161 tcaactaaca aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc
    32221 ggcttgaaac atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt
    32281 ccctataaca cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg
    32341 aatggacctg gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa
    32401 ggaaaagtac cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt
    32461 gcagatacct ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta
    32521 gcgaccggaa gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg
    32581 attgatccag aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga
    32641 gtcaaacaca gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt
    32701 ccgcatgatg cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag
    32761 cagtaaacag atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc
    32821 gatcatcatt ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt
    32881 aaaagaagaa ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga
    32941 taaagaagtg gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat
    33001 tcttcgaaaa gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa
    33061 tatggcaatg gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg
    33121 aacaaagctt tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa
    33181 agaattgaat cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga
    33241 ttttgcaaat aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac
    33301 tggtggaggt atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag
    33361 tctttccccc gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga
    33421 atatgaagtg atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt
    33481 tgacccggtg ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc
    33541 tgaccatgaa tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat
    33601 cgagggaggc tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat
    33661 tgaagtcaat ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc
    33721 gatcgctaag ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc
    33781 tgttacagga acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat
    33841 tcctcgctgg ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa
    33901 agcaactggt gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt
    33961 gcgttcttta gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga
    34021 cgatgaccta atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga
    34081 agctattaga agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt
    34141 ctttttagat aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt
    34201 ggatgacaag atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga
    34261 gctgtgggaa ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc
    34321 tgtatataag atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta
    34381 tagttcctat gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact
    34441 gggatctggt cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc
    34501 tgtcaaagca atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac
    34561 agtttcgact gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga
    34621 tgtgatgaat gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca
    34681 gacagcaatc aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac
    34741 gatcgaagac ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt
    34801 ggatattcct caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc
    34861 ttcgagaatt ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat
    34921 ggaaatcgtt gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc
    34981 tccagaacat ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc
    35041 gatttgtgac ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg
    35101 tgtccattca ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa
    35161 gaaaacgatc gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat
    35221 gaatatccag tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc
    35281 aagtcggact gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac
    35341 gaaagctatt ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga
    35401 aacaaaagaa gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga
    35461 tacttactta gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt
    35521 agacaaagct ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc
    35581 cgtattattt acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt
    35641 tacagaaatt ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa
    35701 tggtctggtc gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt
    35761 cgacttaata agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg
    35821 aaatgcttct aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt
    35881 gtttacttct ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc
    35941 aactaaccct atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg
    36001 aggggacaga tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag
    36061 atttttgaaa tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc
    36121 ttgcatattc tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat
    36181 atcgaccacg aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga
    36241 gtcttctcta ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt
    36301 tttgatttgt ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt
    36361 gtccctcctc tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat
    36421 tttttaggtt ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga
    36481 gaaacaagaa tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta
    36541 ttgttaggaa cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg
    36601 aagacagtgg aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc
    36661 atggcatgtg ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca
    36721 ggaacaaaaa gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt
    36781 atatgaatga gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca
    36841 tgccggcaag cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc
    36901 agcttggatc gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc
    36961 caagagtggc ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc
    37021 tagacgttgt tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca
    37081 tcgcaaatgt tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg
    37141 aagcaccaaa tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg
    37201 gaattgcttt tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag
    37261 tttcttctgt tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg
    37321 ctcaggcaat cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta
    37381 tgcggattga ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg
    37441 gaccagcaat caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc
    37501 ttccaatcat tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg
    37561 ctggagcaag tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta
    37621 aactaattaa agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat
    37681 taatcaaaga agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat
    37741 tgcattagat ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga
    37801 agaaaaattg tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt
    37861 acgttatcta aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc
    37921 gaatacggtc gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt
    37981 tcatgccgct ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac
    38041 tccagaaggt ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga
    38101 agaagaaatg catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta
    38161 tgctagctgt gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga
    38221 agcgatcaaa gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga
    38281 aggaacagtc gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg
    38341 ctcaactttt atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata
    38401 tcaaacgatt caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa
    38461 agacttatta gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag
    38521 tggaatcaaa agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg
    38581 aaaagagatc gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt
    38641 gattgctgga actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga
    38701 tttgccaatg gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga
    38761 aggacgtata tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg
    38821 aagtgtcttg gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc
    38881 ggctatcttt acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc
    38941 tcttatgacg ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat
    39001 cgatgagcaa gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat
    39061 tgaagcgcgc ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga
    39121 atagcaaaaa tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac
    39181 ttctatcgtg atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga
    39241 aagaagagaa taatctcttc tcttttttct attatatata gattttctct attttttggt
    39301 aagggagctg tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg
    39361 atatgatatt cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt
    39421 tattttagga ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac
    39481 agagcataca gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga
    39541 cattgaagaa gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa
    39601 tcatgactac acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa
    39661 agagttaaga gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca
    39721 agagattcca gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga
    39781 aaccgtagaa ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt
    39841 tgatgaaatc atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt
    39901 gactacgatt aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt
    39961 tccggaacta ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac
    40021 gattgtaaag aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa
    40081 aaagaatatg gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag
    40141 tttatgtgat tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac
    40201 caagggggaa gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg
    40261 gtttactgac aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta
    40321 tgtagagttc aagaaagaac gctattattt gacagatatt catttccact tgccttctga
    40381 acatatcatc aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa
    40441 aaaagagaat ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta
    40501 taatcaagaa aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga
    40561 tgtgttcttt ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc
    40621 taccagcggg ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt
    40681 tcttctcccc ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa
    40741 tggaagagaa atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac
    40801 gtggtcgctg tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc
    40861 cagccaagtc tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt
    40921 actcgaacga atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc
    40981 gagaaaatat tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga
    41041 gacaattttt cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta
    41101 ctcgaggaat tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga
    41161 gtgatcgaag aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat
    41221 aatcaagctg gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga
    41281 aaattcaaaa cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc
    41341 accaaagaag aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc
    41401 ccgtattttt atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt
    41461 gatcgaggta ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt
    41521 attgtgattg gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta
    41581 aatgaatttt atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt
    41641 atcaaagagc ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg
    41701 tttattgaac accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc
    41761 tttttgtagc aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc
    41821 tagaaacact cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg
    41881 tcacgataat cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt
    41941 ttcggatatg ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg
    42001 aaaaataggc agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt
    42061 taatgatgac gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag
    42121 ctgtcttcat tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc
    42181 cgtcagaaga aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt
    42241 tcttcttttt caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg
    42301 ggccggcaat ttccagttga gataagacag attcaagata ttggatttca tctttggctt
    42361 cttggatttg ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat
    42421 atttctgtgc attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat
    42481 cttcttcata ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa
    42541 aagtagtaag tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg
    42601 tttgttcacg ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa
    42661 tcagttctcc gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat
    42721 ctaataattg actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct
    42781 gataagcaat cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct
    42841 gatggctgat ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca
    42901 gtaatctttt ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga
    42961 ttgcttttgg atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc
    43021 taaatgggtt caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag
    43081 aacgataagt gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt
    43141 ctcgattaac taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat
    43201 tttgtagatc acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt
    43261 cgatttgttc gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga
    43321 agttaggcgg attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac
    43381 ttgggtgcgc agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga
    43441 tttcattttc ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca
    43501 attcgttgac catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc
    43561 ttttcataag tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt
    43621 aaagcaaaag tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg
    43681 aaatccaaat ttttaggt
//
LOCUS       DAZBTH010000013        43456 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000013 DAZBTH010000000
VERSION     DAZBTH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.013"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..860
                     /locus_tag="ACHGNW_000696"
     CDS             180..860
                     /locus_tag="ACHGNW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6925036.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            950..1984
                     /locus_tag="ACHGNW_000697"
     CDS             950..1984
                     /locus_tag="ACHGNW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6925037.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1998..2333
                     /locus_tag="ACHGNW_000698"
     CDS             1998..2333
                     /locus_tag="ACHGNW_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925038.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2317..4797
                     /locus_tag="ACHGNW_000699"
     CDS             2317..4797
                     /locus_tag="ACHGNW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925039.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4853..6412
                     /locus_tag="ACHGNW_000700"
     CDS             4853..6412
                     /locus_tag="ACHGNW_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925040.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6409..7773
                     /locus_tag="ACHGNW_000701"
     CDS             6409..7773
                     /locus_tag="ACHGNW_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6925041.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7944..9014
                     /locus_tag="ACHGNW_000702"
     CDS             7944..9014
                     /locus_tag="ACHGNW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925042.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9029..9697
                     /locus_tag="ACHGNW_000703"
     CDS             9029..9697
                     /locus_tag="ACHGNW_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925043.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9717..10295
                     /locus_tag="ACHGNW_000704"
     CDS             9717..10295
                     /locus_tag="ACHGNW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6925044.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10698..11609
                     /locus_tag="ACHGNW_000705"
     CDS             10698..11609
                     /locus_tag="ACHGNW_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6925045.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11865..12782)
                     /gene="rnhC"
                     /locus_tag="ACHGNW_000706"
     CDS             complement(11865..12782)
                     /gene="rnhC"
                     /locus_tag="ACHGNW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN6925046.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12984..13421
                     /gene="zapA"
                     /locus_tag="ACHGNW_000707"
     CDS             12984..13421
                     /gene="zapA"
                     /locus_tag="ACHGNW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN6925047.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13479..14026
                     /locus_tag="ACHGNW_000708"
                     /pseudo
     CDS             13479..14026
                     /locus_tag="ACHGNW_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            14141..16501
                     /locus_tag="ACHGNW_000709"
     CDS             14141..16501
                     /locus_tag="ACHGNW_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN6925048.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16588..16902
                     /gene="trxA"
                     /locus_tag="ACHGNW_000710"
     CDS             16588..16902
                     /gene="trxA"
                     /locus_tag="ACHGNW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN6925049.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17084..18862
                     /gene="uvrC"
                     /locus_tag="ACHGNW_000711"
     CDS             17084..18862
                     /gene="uvrC"
                     /locus_tag="ACHGNW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN6925050.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19007..19906
                     /locus_tag="ACHGNW_000712"
     CDS             19007..19906
                     /locus_tag="ACHGNW_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925051.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19899..21161
                     /locus_tag="ACHGNW_000713"
     CDS             19899..21161
                     /locus_tag="ACHGNW_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925052.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21324..23222
                     /locus_tag="ACHGNW_000714"
     CDS             21324..23222
                     /locus_tag="ACHGNW_000714"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN6925053.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23521..24240)
                     /locus_tag="ACHGNW_000715"
     CDS             complement(23521..24240)
                     /locus_tag="ACHGNW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN6925054.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    24430..24477
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24577..24909
                     /locus_tag="ACHGNW_000716"
     CDS             24577..24909
                     /locus_tag="ACHGNW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925055.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24896..26899
                     /locus_tag="ACHGNW_000717"
     CDS             24896..26899
                     /locus_tag="ACHGNW_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN6925056.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26918..27388
                     /locus_tag="ACHGNW_000718"
     CDS             26918..27388
                     /locus_tag="ACHGNW_000718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN6925057.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27409..27993
                     /locus_tag="ACHGNW_000719"
     CDS             27409..27993
                     /locus_tag="ACHGNW_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFN6925058.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28008..28994
                     /locus_tag="ACHGNW_000720"
     CDS             28008..28994
                     /locus_tag="ACHGNW_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN6925059.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            28984..29295
                     /locus_tag="ACHGNW_000721"
     CDS             28984..29295
                     /locus_tag="ACHGNW_000721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN6925060.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29317..31098
                     /locus_tag="ACHGNW_000722"
     CDS             29317..31098
                     /locus_tag="ACHGNW_000722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN6925061.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31091..32467
                     /locus_tag="ACHGNW_000723"
     CDS             31091..32467
                     /locus_tag="ACHGNW_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN6925062.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32474..33109
                     /locus_tag="ACHGNW_000724"
     CDS             32474..33109
                     /locus_tag="ACHGNW_000724"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN6925063.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33139..34494
                     /locus_tag="ACHGNW_000725"
     CDS             33139..34494
                     /locus_tag="ACHGNW_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6925064.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34686..36080)
                     /locus_tag="ACHGNW_000726"
     CDS             complement(34686..36080)
                     /locus_tag="ACHGNW_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6925065.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36172..37143
                     /locus_tag="ACHGNW_000727"
     CDS             36172..37143
                     /locus_tag="ACHGNW_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN6925066.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37140..37922
                     /locus_tag="ACHGNW_000728"
     CDS             37140..37922
                     /locus_tag="ACHGNW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN6925067.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38091..38462)
                     /locus_tag="ACHGNW_000729"
     CDS             complement(38091..38462)
                     /locus_tag="ACHGNW_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6925068.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38596..39039
                     /locus_tag="ACHGNW_000730"
     CDS             38596..39039
                     /locus_tag="ACHGNW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6925069.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39082..39891)
                     /gene="yidA"
                     /locus_tag="ACHGNW_000731"
     CDS             complement(39082..39891)
                     /gene="yidA"
                     /locus_tag="ACHGNW_000731"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN6925070.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39910..41277)
                     /locus_tag="ACHGNW_000732"
     CDS             complement(39910..41277)
                     /locus_tag="ACHGNW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6925071.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41261..42091)
                     /locus_tag="ACHGNW_000733"
     CDS             complement(41261..42091)
                     /locus_tag="ACHGNW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN6925072.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42254..42691
                     /locus_tag="ACHGNW_000734"
     CDS             42254..42691
                     /locus_tag="ACHGNW_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN6925073.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42767..43387
                     /gene="rpoE"
                     /locus_tag="ACHGNW_000735"
     CDS             42767..43387
                     /gene="rpoE"
                     /locus_tag="ACHGNW_000735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN6925074.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14237 a         7474 c         9224 g        12521 t
ORIGIN      
        1 attgaaatat tttttcatac ttaataaaaa aggaagaata ttcatgtaaa cgattttatt
       61 gtttttttct cttttataca aaaagggtca taatttcaag taagcggatg aattcatgag
      121 gattttttgc atactgtctc tactatactt aggtcactaa gaaaaaggag tgatttatta
      181 tgaaaaagaa catgattggg acattattac tttcaacact tcttctaggc ggcttggcaa
      241 caccagcttt tgctgagggt caagcgactt caaaaggaga tattaccttt actgaaccaa
      301 caaataccgt tgagcctctt aaccctactg atccttcaaa accagtagag ccagcagatc
      361 cggaaaatcc agcaactggc cagacaggtt ctttaacatt ggatgtagtt cctgaattgc
      421 catttggtac acacgagatt gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa
      481 acgatacacc ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac
      541 aagggtggaa cgtgacagtt tcggtttcag attttgtaaa cggttcacaa gttttgcaag
      601 gcgctgaatt agattttgga acatccactg ttaaaagtac gtcagataac gaatccactg
      661 gaccaactag tcaaactgta actgggttga gtaaagcaag tgcagcgaca ccaatattta
      721 cagcagcaaa agatcaaggt ctgggaacat ggctttctgt ttatgatcca gcaaatatca
      781 cactgaaagt gccgaaagca gcagctggaa cattcacggc tgatttgact tggaacctcg
      841 tggctggccc agtagcatag agtatgaaat aaattcaaca aactgcggtg aaaatactat
      901 aggtgaagga ttttttaccg cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag
      961 taaacgactg tttgtttcgt ttttttctat tttaagtcta tttttcattt ttccgtcaat
     1021 cagccaagca aaggattcag caggagactt tggaatcaaa ccggtatttc cagagaacca
     1081 aatagataaa gcaatcggtt attttgattt attagtggca ccggaacaaa atcagacact
     1141 agaagtaatt atcagcaatt cttcagatga agaacgaaca tttgaggtct ctgtcaatcc
     1201 ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa cgttagatga
     1261 gacattgccg tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc
     1321 agcacatgca gaaacaacta tacctataga agtgaaaatc ccagcaaaaa gctttaaggg
     1381 acgtgtatta gcgggtattc atgtttcacc taaagaagaa gctgaaacag aaaatgctac
     1441 agaaggagca caaatcaaaa atcgtattgc ttataatctc gcagtcgttt tacaagaaag
     1501 tcaagagacg atagaaccgg atctgaaact tttatcagga gatttggacg aggtcaacgc
     1561 aaagccaact gttcagcttc gtttccaaaa tcctcagcct aggattatca gcaatttgat
     1621 atttacgagc aagatctttt atgaaaatca gctttatatt gaaaatacgt ctaatgaatt
     1681 tttagttgca ccaaattcga attttcactt gaatcttgat ttagctggtg ataaagcaaa
     1741 agccggcgat taccgcgcag aaatcatagc aaaatcaggc gacagtaatg aatggcgatt
     1801 tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt
     1861 tgcagtacaa gaacaatctt tcccatggct ttatgcagga ttggttgctg cagttctagt
     1921 gttatggttg attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac
     1981 atgatgaggt gatttcgttg aaaacatcga tcttattggg agggattatt gcaagtttgc
     2041 tgttcttcag ctggcaagag ccagtccaag cagataccaa aagttcaggt acgattacat
     2101 tttatgatct aagtccagtt ccgcatccaa gtggttcagt caatggcgca tcaaaacagc
     2161 agacacagct accgatttat aaagaatcta atcaacatgg cgtattcccg gagacgaatg
     2221 aagaaaagat ccgttttctt tatttgatca ttggtataga attatggctt cttgcgtggt
     2281 tgttgttatg gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa taaattttca
     2341 ttttttcgct ccttcttttt tacttcgctt tttgttttaa gtatatttat cttttcacct
     2401 tcggcagaag ctgttggagc aacgcctcct gccatttctt atgatccata cacgtatgaa
     2461 aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct tgtccaacta
     2521 actggggcta accaattgcc taaattgact gctacttatt tgaacgatgg ttcaaatgat
     2581 ccttggaaag gtggcacggt ccgttttcat tgggaaagcc atgtgaaact aacaaatgta
     2641 cttgatttgg tgaatatcac tgcacaagga tttgcatttg tggcgatcat caatttgccg
     2701 gaacatgtat caagcagtga tgtattaaaa gcaattgact ggaacaacgc ctacttgtat
     2761 attgccggtg cccccatcaa ggttactagc ggtagtttat tatcagcagg aagccatacc
     2821 ttgcgttttg ggatggggtc gtgggataca aatactctat tggcagatat tattgctatt
     2881 attacgcgag gcgaattcaa cgtggacgat atcccgcttc aatttgatgt tacagctaat
     2941 gtcgcgaaga tgacagaaaa cggagataaa tatgatacga gtccaaacaa aattttgacc
     3001 aagggtaaat tacaaccttc tatgtctaaa aaggccgttt tttctgttga tttttatgat
     3061 tcgaataata ttatccaaga tcgtatgttc aattcgattt tagctccagc tgcaggtaga
     3121 ttttatccta aattaactgg cggaaacttg aatccaggag catatgcatt ttctgcaagt
     3181 gcagagataa atacgtggaa tagttacatc tcaacaagtg attcaacagg aaaatataat
     3241 acatttgctg ataccacaga atacgtggat ggaaataacc agaaccttcc aggctctacc
     3301 aatagtcgga cattgacgat ggggctgact gaaggacgtt ttgatcagtt gccatttagt
     3361 cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg agcaatactg
     3421 actcatactc cagcaagcac aagtggattg aatcagacag ccaatatcgt gtacagtggg
     3481 aaagatgcgc aaggcggatt gctttctcct gttgctttaa aggtattgga gcagtacaag
     3541 ctagacggaa cggttttacc aacaaactta acagccgata gtcagtgggg aaagctcctt
     3601 tcttctgcat cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa aaagggttcg
     3661 atccattaca ggctttattc gaaaggaacc caaaaattag cgacaggatg gacagatcaa
     3721 cttttccaaa cgatccaatc aaataacggg ggtgtaaata cagctacttc tacactacct
     3781 gcattggctt cagggcaata ttattttgat tatcgcttgg tggacgatgt acttactaac
     3841 atgtatccgt cttttgcgta caaatggcag tctgaacaag ctggaatcac cactcttcct
     3901 caaataacag tggctaattt tccatctgtg agtgcagata gtcagctaaa aaaccttagc
     3961 agacaacctg agacaggaga tcccctgtct gctctatcag gagatacgat tcaagaaaat
     4021 gtgacattca cgttaacaaa atctggggat gcgataaccg acaaaaaaat cacgatctca
     4081 cttcctgaaa atactactta tatcaatggt tccttaaaat taaatggtac aactatcgca
     4141 gataccggca ttcaacaagg agtttctgtc ccagcagatc tattgagcaa aataggagat
     4201 accattcatc ttacttacaa ctatcaatta aatactgtag atacttctgt acaatccgtc
     4261 agcatactga caaaggcagc tgtactttcc agcaatatta cattagcaga tggtgctaag
     4321 cttcctaacc cagttgtcca aacatctgct aaaacaattc ttgtccccaa acaagaatta
     4381 acattagtta atgtaccaga tgattttaca tttggtaacg atctccccaa accgttgaag
     4441 acaagttatt atgaagcaaa aggcgatttt tcttttgatg tgcgggatac tcggcttcca
     4501 agcacgtctc cttggcaact gacagggaca ttgacttctt tatttaagaa tgatcaaggt
     4561 caagaattaa gcggaacaaa attatacttc aatcattctg gatcaaaaca attgattcag
     4621 caaggtcaaa acacgttgat ttatgagagt gatggaacag ctaaaggaga ggttctcgtt
     4681 gactttcccg ataccgatgg tttactctta gaggtaaata gcagtacgaa tgctcaacct
     4741 ggcgcaacgt atcaaggcat ggtcacatgg gaactgacag ctgggccaac cagctaagtg
     4801 attagaattt gtatggatct gtgataactg tcacagatcc tctttttcaa ggatgagaag
     4861 gaaacttctg ctgttaaaat cagaaatgct tggaggagtg gacttggaac ttttcagttt
     4921 gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa cttttattag aggaaaaagg
     4981 agagatttcc gttaataacg tacttacccg cttatcagtt gatcgggcta ccttgaaaga
     5041 agatctgttt tatttacaag ctaacttgga aaatatcaaa caattggaaa ttgttatcca
     5101 agaagggaat attttattaa tcagacatgg aaatatagca gcagtcgatg tttattatca
     5161 ttgctacttg cgctattcta caaaatatca gatacttctt tatctgttgg aaaatggctc
     5221 atttgatcgg cagaaacttg tcatggaact taatatcagt tcggctactt tgtctcgcag
     5281 agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa gcgggcggct
     5341 gaccggtcca gaaagtcaaa tttgttattt ttatttccaa ttattttggt ttggacgtcc
     5401 ttatcttgaa aacaaaaaag aatacagtga tttatcagga aacacttttt tagatctgct
     5461 gcaaaaagag tttagtttta ctattacaga agaaggacga ttgaaatttg aaatatggct
     5521 ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct
     5581 tcctgttgga ttgtttcaaa cgacacaatt tttattggac ctccatacat ttttgacccg
     5641 ttacttcttt caatatgcct ttccttggaa tcaaaatgaa acgttggttt tttatctttt
     5701 catgatcagt aactttacat tagatacaca taactcgcat gtccaggagt ttttgataag
     5761 cgaaaaatac aaagacgacg tagtagctca attgaatcga acgtttaaac aatcgataga
     5821 agaatatttc tctttttatg tttttccaga agagatttcg caaaagattg cgttgacagt
     5881 gatgcaaaat cacgctcatt ttgcctattt tagaggatgg atgaatattt ttggccaaga
     5941 aggattaaaa cagcggttaa ctgaagtaga agataaacaa ttcctggttt tatgtgaaag
     6001 attggtcaga caaacatttg ctattgttga ttttactgaa gtagacgagg aaaatgcccg
     6061 ctttgaatta tttgctcgct atgcatctat tttgcggacg ctgatcaagc agttagaatt
     6121 acgtctgcaa attgcttatg atcttcctta tgaaaggcta atggcggata tcgtgaaaga
     6181 aatcatccag gaacaattgc tgaatacgat caaatatgat ttagtcgaat acgagaaaga
     6241 aaagcagtat gatatcattt tgactagcca attgaaagag tacccgtcac agaagtccgc
     6301 taaagttttt gtattcacta gcaacgaata taattatgat ttcccctatt tgaaccaatt
     6361 tttaaaagaa tgttatttag aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg
     6421 atcattggtg cttcccatgc aggcatcaca gctgccctaa atctaagaaa acttcaaccg
     6481 aaagcagaag ttttactgat cgatgaagat cataaagatg gactaggcta tgtttcaaat
     6541 ggaatcaacc tttatttaaa aggaaagata cgtagtttag ctgaagttgc ccataacatg
     6601 cggactcttc aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac tgaacttgat
     6661 ccagacaaac atcagctcat cctcgcatcc aaagaaggaa aaaacgagac cataacctat
     6721 gataaattaa tcgtagcaac aggttcctct cctgtcacct tgtataaaca aattgaagct
     6781 gaaaatgtct acacctataa aaatctagtg caatctaagc aagtacttgc cgctttaaaa
     6841 gaagcaaaag aagtagtgat cttcggtgct ggttatattg gattggaatt agcagatgct
     6901 ttgagaaata aaggatacat tattcatcta gttgattata tgccgaatgt tttatcaaga
     6961 tatttcgata aggatatgat caacagtttt caaaatcagc tgcaaactaa gcaaatcaat
     7021 ttttatccaa atgaattctt gatcgattgg aaaaaatcag aagagaaagt agtatccgta
     7081 caacttctat ctcaagcaat aaaggcagat atggtcattt tttctgccca aactcgtcct
     7141 aatacgacat tgttaaaaga aaaagtggct ttgtatgagg atgagactgt gatagtcaat
     7201 gaatatctgc agacaagtga tccggacatc tatgcgattg gagatatcgt cccagtcagt
     7261 tttgacaaaa ataaacggca tctcttttta cctttagtta ctcgagctgt acatatggca
     7321 agagcagtag cgctaacttt gagtggtcag ccaaccgctt acgacctcag acaaaagata
     7381 acagcagcag tcattataga ctacttttta ggaactgtag ggctgacaga agatgaagcg
     7441 ccattcttgg aacaaaatac cggttcgtgt tcaggagaat ttgatctatt tccacaatac
     7501 gatgaagaaa acaaaacagt aaatgcaaag ctgatctacc atccggatac attagaaata
     7561 attggcggac agctgataag ccaagaattc ttattatcag atttgaacct gcttgcggat
     7621 atcgtgaaac acaaaactac gattccacag ctagcagttg aggattttgg atttctgtct
     7681 gaacacacct ctcgttttca ctacctcaat gagttagctt ttaaagtact agctaagaga
     7741 gcaaatagca atagagtaga taaaagactg tgagaaatat cacggtcttt tattgtgtgg
     7801 ggatataata aatattaatc aatcgagaat aaaaaattca tataatgaaa tagcggaaga
     7861 attttttgag cataatattc gccaaaaatt tatcaagtga taaaattgtt gtataaaatg
     7921 attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg cattcaaagt
     7981 tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta ttttttttga
     8041 ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag tgggaggcag
     8101 attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg attactaaga
     8161 agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccagaca cctggagtcg
     8221 tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata
     8281 aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac gatgatgagg
     8341 cagtaggcga tattagtttg aaagaagaat atggtctagc cgtaggagat acagttaagt
     8401 tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc aaattcaatg
     8461 tctcaccagt tctctataca accatcaatg cttaccaaga gatccgcttc gaaaaagaag
     8521 atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc agtaatttac
     8581 ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct ggatataatg
     8641 cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg gctattgtga
     8701 tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt gtaatgaaag
     8761 cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca ttcattttat
     8821 catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt gttctacctg
     8881 atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg ttgatgctgg
     8941 tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc gatccgttga
     9001 aggcaattgg ataagaagga ggaagagcat gaaagcaata gaatttaaag aagtagaaaa
     9061 aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca tgagtatcga
     9121 taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta cttttttgac
     9181 tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg atcaagaatt
     9241 cagtaatcaa aaagaaagtg aacgcgttaa actacgtttt aaagaaattg gctttatttt
     9301 gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat tagtagataa
     9361 gatcaaacat gaacatcgtc aaaaagaagc aaaagaatta tttgagcagc ttggcgtgga
     9421 taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa gggtcgcaat
     9481 cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa ctgctagttt
     9541 ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa aagacaaaaa
     9601 taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg atagaatatt
     9661 tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg
     9721 tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact gacttgttta
     9781 tgtccaaggg atatctcggc acttcaacaa gagagatcgc acaaaaagtt gggatcaccc
     9841 aacccaatct ctatcattat ttcggtgata aagaaaaact atatacggca gttttagaaa
     9901 accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc gaaacgggat
     9961 ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg gtcgatttgt
    10021 ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat catcttttct
    10081 ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca aaccaatctg
    10141 tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta gtgttagcac
    10201 cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg caattgatcg
    10261 atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg agaaaaatag
    10321 ttaggtgaag aagcaagata tgacgaagat cgttatatct tgtttttttt atataggtag
    10381 tttttcataa atacaaattt gttattctct catgattatc agggtatatt tttatttatt
    10441 atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg tttgtagaat
    10501 gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga gatttcagga
    10561 tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg ttatatcttt
    10621 tacagcccct tttttgcgta cttaagctga ttttaagttt tgacccttag tctggatcta
    10681 cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca atcattatca
    10741 ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga caaacggtca
    10801 gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca gaacaagaga
    10861 cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg ctaactaccc
    10921 cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca aaaaatgctt
    10981 atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat ggaaccaaag
    11041 tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt gtattagatc
    11101 ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca gaagaaaaag
    11161 atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct ggagcaacgg
    11221 tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc gactacagtg
    11281 aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa tatgcgaatg
    11341 aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta gcacagacaa
    11401 ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact gggaactatc
    11461 aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat atgaataatg
    11521 atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct gcaagtttga
    11581 cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa aaatagctcc
    11641 tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa caccgtttaa
    11701 ggaagaggtt gtgagcctgc cgtttaactc cgcgtcacaa ggagcaattt caaaaaacag
    11761 cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcatga gaacaccgtt
    11821 tattcggaac aagggactgt gacatgactt ttgtcacagt tccattattt ttttagcagc
    11881 ttcaaagctt tttctgtatt ggcaaaatga agtttggcat atttttcaag cataggcatg
    11941 ccgcctttct ccaatagttt tgccgctact tggtcagatt tcgttccagc tcctgatgga
    12001 agggtgattc cagcttcagc tgatgctttg tgtaactctt ccaaaaagct ggcacgactg
    12061 atgatagaag cagcagcaac agctaaatga tattgttctc ctttagtaac gaagtacagt
    12121 ttttcttgta attgattttt ttctaaacgc gcatattttt taaaatttgc ttccggcgta
    12181 aactgatcga tcaaaatcgc ttctggtttt tctggctcga ttttttttag cagcaaatgg
    12241 atcgcttgat tgtgcaaagc gactttcatc cgaacggcat tgtattttgg ttggatctca
    12301 ttgtattttt ctggagataa gacaagcagt ttatagggga tggttttttt taagatgtca
    12361 gcaagctgga tgatttggct atcttttaac tcttttgaat ctctaacacc aagtttacgt
    12421 aattctgcaa gctgatcacg tttcacataa gcagcacata ctgttagcgg accaaaatag
    12481 ctgccatttc cgacctcatc actacctaaa acggataatt gactgatatt ttttggcaaa
    12541 gaagaggctg tttttgctgg cttggcttgt ttatcctgtg cctcccccca tttatcagct
    12601 tctattgctg cattcgtacc ttgaaacatc acttttccgg atgtataagc ggtaatggtc
    12661 gtcccgtctt tttttgcagc aaagctagta taaggaatct gtttatttaa caaataagag
    12721 cgatagtatg ctttcatttg actaagttgg tcgtttgttg ctttgataat gacgttgctc
    12781 ataaaattct cctcgttttt atacataaaa ctataccgca actcacgact tttcgttttc
    12841 taagagattg cgagacatag cattttttta aagtttatca tataatagtt aagtatgtga
    12901 aaaatatttg tggagaacgg tgaaagaacc aacttcatgg tacactaaat aaaactttca
    12961 tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa gcagtgattg
    13021 ccaaccaaac atatactatt atcggacggg aaaccaagca tcatatggat atcgtaacga
    13081 agctgatcaa tgaacagtta gcagaattga aacaattatc accacaaatg gataatgaac
    13141 aagcagcaat cttgatggct gtcaatgcgc tttcggatca attgaagaaa caagaaagaa
    13201 tcttggagct ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa tttacagagc
    13261 ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa gtattgaaag
    13321 aaaatggtca agcagatcat accattaaaa atcatgtgga agcacaacag attttaaacg
    13381 agaaacgaaa aggacaaatt aaacaaaaat cttctaatta agatcctttt atcaaaataa
    13441 gaaaaattta aaaaagttag aagagaaagg aacgtagcat gctgagtttg ctgattttat
    13501 ttttattgct aattgcgttt ttttctggag cacgtcgcgg gtttgctttg caaatcatat
    13561 tcgcgattgg atatgtattg tcatttattg ctgcccagca tttttacaaa ccattagcaa
    13621 gtcatttgga attatacatt ccatacccag ctgtcacccc aacatcaaag ttagcatttt
    13681 ttgatcaggt atttgctttt cacctagatg aagcttttta tgcaggaact gcttttctga
    13741 ttattttgct aattggctgg ctgcttaccc gatttgtggg ggtttttgtc catggattga
    13801 cgtatgtgcc gatcttgaga caggtagact ggattgcagg aggcatcctg tcactaatca
    13861 tggcttacgt tactatattt ttgattttgc agttacttgc ctttgttcca ttagtgtggt
    13921 ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa cgccatttct
    13981 taccaacaag atgtatgagc tttgggtaac gaatattttg aattaaggaa aatagtgctg
    14041 gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta gcgtatgtcc
    14101 ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc gaatccttga
    14161 aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg
    14221 aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca caaactggct
    14281 tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa
    14341 acttgaaaat atccgtcccc atatgaaacg gatcgagatc ggagcagact tgaatggagt
    14401 ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc gtttcattga
    14461 tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag atcagttagt
    14521 cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac
    14581 tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa gtgaacaagc
    14641 tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc
    14701 gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat accgcggtgt
    14761 atttggcggt gtagtccacg atcaaagtgc ttcaggacag actttattta ttgaaccaaa
    14821 acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac gaaatgagat
    14881 tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag aaatcatcca
    14941 caatgcttat gtgattggta aaatggatct gatgaatgca aaagcacgat ttggtaaaga
    15001 agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga aacaagcaag
    15061 acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg gaaaagacta
    15121 tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt tgaagacttt
    15181 aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg aagaaagtca
    15241 aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga ttgagcaaag
    15301 tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa aagtcaatga
    15361 aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc
    15421 tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga tggcaacaac
    15481 gcattatcct gaattgaaag tttatggata caatcgggca aatacgatta atgccagcat
    15541 ggagttcgat gtggatacac taagtccaac gtatcgtttg ctgattggcg ttcctggacg
    15601 aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta ttgatgaagc
    15661 gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag atttagagaa
    15721 tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca
    15781 agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac gtgaaaaaga
    15841 aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca gaggcggaag aaaaagcaga
    15901 aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga
    15961 gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag aaactttgaa
    16021 gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt
    16081 gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga atcagtggca
    16141 agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc
    16201 tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta gtagtcacgt
    16261 gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg aggtagatca
    16321 atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc acggaaaagg
    16381 aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga gcgtcaaaag
    16441 ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta aatttaagta
    16501 aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct atactaatca
    16561 tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa gacatttaat
    16621 gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg tggtccatgt
    16681 cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga agaattcaga
    16741 attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg tatcatgagc
    16801 attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt tggtgttcat
    16861 tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac agcaaaaata
    16921 agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg agttaaggtt
    16981 attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt tttttgacag
    17041 acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg aacgaatcaa
    17101 aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg
    17161 aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct catattttac
    17221 tggcagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact ttgaatacat
    17281 cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa agaaaaatga
    17341 tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta agataacgaa
    17401 tgaaaaatat ccccgtttgg tgattactcg taaagtacta aaagacaaag cactgtattt
    17461 tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg atcgattatt
    17521 tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact atcatttggg
    17581 acaatgtctg tgtccttatt atttcgacgt agatccgaaa gtctatacga aaatcgttga
    17641 tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc gagtcagaga tccatcagaa
    17701 aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc gagaccagat
    17761 ccgtgccatt gagacaatca tgactcgcca aaaaatgaca aatactgatt tactggatcg
    17821 agatgtattt ggttatgcga ttgacaaagg atggatgtgt gtccaagtgt ttttcgtaag
    17881 acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg aagcagaaga
    17941 agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca ttcctaaaga
    18001 agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt
    18061 gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgaata agaatgcctc
    18121 agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga cgatcggagc
    18181 agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg agtcttttga
    18241 taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa
    18301 accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac cggatgatta
    18361 tgcatcaatg agagaagtca tctatcgcag atattcacga gtgattcgcg aagggttgcc
    18421 tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt
    18481 cttagaaaat caattaggaa tcgatattcc tattgctggc atggcaaaaa acgataaaca
    18541 taaaacaaat gagttgctat tcggtataga tttggctgta gtaccgcttg agcgaaattc
    18601 accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg caatcacttt
    18661 ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa ttgaaaggtt
    18721 aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc
    18781 agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt
    18841 tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc
    18901 attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa tcgcttacgt
    18961 ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa
    19021 ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt tccaaatccc
    19081 cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa cgacttttcg
    19141 tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg gccatcatct
    19201 tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac tttatccgaa
    19261 agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat caagaaaaga
    19321 gattgaacca aaaatcgatt tttggatgga aaaatttcag gtaaaaggga aaaagacgga
    19381 taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat
    19441 ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc cggtaaatgc
    19501 tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg tcattttttc
    19561 tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg
    19621 aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc gtacgaagtt
    19681 attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt
    19741 gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag ctgggaaaga
    19801 aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc aaccgccaac
    19861 tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt tggattatta
    19921 ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg atattagtcc
    19981 cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa aatagtgatg
    20041 tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg agccaagtca
    20101 aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa aaagagcttt
    20161 cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag ataaatggga
    20221 tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa caacaactga
    20281 ctggcttgca atcgatgctt cgagcaagtc aattaggcat ttcaccagaa gaagtagcta
    20341 gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa
    20401 tgacagttgg agaagataac tcgacagtcc aatatgtcgt aagttatgta gcaacgattg
    20461 tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc gcttctgaaa
    20521 aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag catttttacg
    20581 gaaaactaac aggtgtttta cttgttgcat taacgcaaat ggcattatac ggtattattt
    20641 ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata ttagacggta
    20701 tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc atgatctttg
    20761 gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac aaagcagaag
    20821 atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatat atgcttggat
    20881 tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg tatattccat
    20941 tcttatcttc atatattatg ccggttcgct tagcaaatga gacagtcggt atgggtggag
    21001 cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc ttgtctgcaa
    21061 acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg attatggagt tcattgaaac
    21121 aatcgatttc aattatgaga aatgaacgaa aaaaagcata aaatatagaa aaatatagaa
    21181 ggaagctgga catttattgt tcccagcttc ttttttgtat agacctattt ctattttttt
    21241 attatattgg atatatatat tcattgaaaa tagagcataa caactttata atgttgagta
    21301 taccaatttt tggaggcgtt ttaatggacc gctatttaga tttactttca gagaagttcc
    21361 ctacaacgga agatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa
    21421 aaggaacaga gttatttttg agcgatatac atggcgaatt tcctgctttt gatcatattc
    21481 tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttctct gaacaaatga
    21541 cggaaacgga gatcagccaa ttcactattt ttacggctta tccagaatat gtgttgacga
    21601 ctgattggta caaaaaacaa gataaatccc agctgatcca tcgtttgatc gatcttttac
    21661 ggtttacgac agtcaaatac actcgatcaa aagttcgaaa aggattgccg aaagaatata
    21721 gctatatcat tgaggaactc ttgtatatcg atgatcgtat caacgggaaa aaagactatg
    21781 taaaaaaaat cattgaacaa cttgtcttga tgaaggaaga agaaaaattt ttaacaaaac
    21841 ttgcgcagac gatccaaaaa tctgtcatcg atcatttgca tatcgtgggt gatatttttg
    21901 atcgaggaac tcaagcagcc aaagtaatgg atcgaatcat ggatttttcc tctgtagata
    21961 tccagtgggg gaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt
    22021 tattgacgct tttacggatt gccgcacgct acaactattt atttgaatta gagaaagaat
    22081 atggattaaa tttacgtcca ttattttctt ttgcccatca aacctatcag aagaatccag
    22141 tatttacccc taaaaacagg aaagatctat ctgaaagaga gcaagaagaa ttagaaaaga
    22201 tccatcaagc actttctatc ctccaattca aatcagaaca tcagttgttg aatcgacgac
    22261 ctgaatttaa aatggacgat cgtaaattgt tggaaaaaat caactatgag gaatcaacga
    22321 tcgttttaga aggtcagctc tatgagttga aagggacttg ttttcaaacg atccagccag
    22381 atgatcctaa aaaattcttg gaagaggaac aaaaagtgat cgatagtttg atgtattcat
    22441 tccagcactc gttaagaatg caaaaacaca tgacgttttt gatcgaaaaa gggggaatgt
    22501 atctcacgaa caatcagcat gtactgttcc atggctgtat acctgtagat gaaaaggggc
    22561 agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg ggattttttg
    22621 aaaaacagat aactgaaagc gcaaaagatt tgactgcaaa tgaagatttg gcgaccgatt
    22681 tgatttggta tgcgtggagc gggccatttt cgcctttatt cggcaagagt aaaatggcaa
    22741 cttttgagcg ctattttctc acagagcctc atacgcacgt cgaaaaaagc aatccctatt
    22801 atcacttgcg agatgaagag tggttcagtg aaaaaatttt ggcagaattc gatgcaaaag
    22861 aagaaggttc agcaatcatc aatggacaca cgccagtaaa agtaaaaaaa ggagagtcac
    22921 cgatcaaagc aaacggaaaa gcatttgtta ttgacggagg tctatccaaa gcttatcaaa
    22981 aaacaaccgg gattgctggc tactcattac tatgcaattc ttacggtttc caaatcgtga
    23041 cacattatcc gttcttgcct attgaacaac aatttgcaaa aaaatctgat caaacgaatg
    23101 taaaaaaagt gatcgagcag cagctcccac gcaaacttaa tcggaatacg acaaaaggaa
    23161 tggaattaca gcaacagatt actcaactcc aaaggctgtt ggattatcga aaggacgatt
    23221 gagaaaaatg ctagccaaca ttgctcaata gactaaaaag cgactgcaac aaaaggcgtg
    23281 tcgcagtctc cttcggaatt agctcaaaaa acggtaaaat attagaagct atttttctgt
    23341 ttatttctct aatactcagg tcagggcgct gatttcacga cctcttcctt aaacggtgtt
    23401 ctcctgccaa ctcctcggtc acaagtcaca atatccaaca aacatgagaa gctgtttttt
    23461 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctaatc agaaaaacga
    23521 ttaaaattca ttcacaaaga aatcgaataa acgctgttca gatgaatcta cacgatgaga
    23581 aagttccgga tgccattgga caccaaggat tttgtgcata ttatctttac tttggactgc
    23641 ttcaatgagt ccatcgttgg aaaaggcgat ggcttttaac aaaggagaaa gatctttgac
    23701 tgcctgatga tgataggaat tcacaaagta tgaatcaggg agaaggtcac ttaataaact
    23761 gtccgaaacg attttgactt cgtgagttgc aaactgcggg gcagtgggct gttgttcgtg
    23821 ttttacggac cattttggat ataatgaaag atcttgataa agtgttccct ccaacacgac
    23881 attcaatagt tgaagtcctc ggcagactgc aaaaatcggt tttctttgtt tcaatgcttc
    23941 caagatcaat gcctgttcga attggtctcg ttgaatattt gtttccgtca gttttggatg
    24001 agggtcttcc atgtaaagtt gcggactaac atcctgaccg cctgcaagaa tgagtttgtc
    24061 gattttctca attagttgag gcgctgaatc tggagacatg actggcagaa taagaggtaa
    24121 tccttgagca tctaaaactg catccacaaa tccctgcggt gtatagctta cttgatttcc
    24181 ttggaaagta tctgtcgctc gaatcagttg attcccggca atgccgatga ttggtttcat
    24241 ttttattccc cctaaagtag actaattgta acaaggattt aaaaaaattt aaactatgta
    24301 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaattttcgt
    24361 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    24421 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    24481 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    24541 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    24601 gaacaaatcc gaaaagcaga agaagaaaat gagaagcgtg ctcgaaccct acggcagcag
    24661 ttgaagacct atgcagaaga aaaagagcaa gaattggaga taatccaaac gaacagtcgg
    24721 caacagctga aacagttggt agaagaaaaa gaacaattcg aaaagcaaga attagaaaaa
    24781 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agaccaatat
    24841 caaaagcatc agagtcaagc aatcggtgca atcattgaaa gggtgaaaga aacttatggc
    24901 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    24961 acaagcagta caaggaatcc aaaatgttga aatccgggat ctgtatcaaa atagaacaaa
    25021 caaccagtgg gtagaaaaat attttccaga taccacgatt attgataaag aagcaaaatt
    25081 aagcgcttta ggcaatcggc tgaaagacat ccaagaagct atccaattca tacaacacca
    25141 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagactt
    25201 ggaagcaaat tattccgaag aaaatttcat gaataaatta gaagaagtcc ttgatttaaa
    25261 agaaaagtgg gaacagctaa ctgaaaaacg tgatcgttta gaagaagagg aagaatggct
    25321 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    25381 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac taagtaagct
    25441 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    25501 tgctttaaaa aaatcagttg ctgctataga agaaattgct gggaaatacg ggttcaataa
    25561 agaaacgtat ccttatgaac aaaatcctaa agaaaaattg caagaaacca aacagcaatt
    25621 atccattgtt cacgagcagc aaaaaaagat aacgacagcg ttaggtcagt gcagcggcta
    25681 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gggaaaaagt
    25741 aaaagaacgg tttatccatg ccacttatct gatcgttttg caaggttggg tagatgcaga
    25801 agaaaaacag aatttgatgc atgtgttaaa cgaaactgta tcagaagaag atatatacgt
    25861 tagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    25921 ccatccgctt atcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    25981 agaagtagat ccaacgcctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    26041 cgcagatgtt ggttacggat tgctgatgct cattgggaca atactagctc aaaaactatt
    26101 tgttttaccc cgcgggatga aacgtttcgt aaaattcttt gagattctct cggtaccatc
    26161 gatcatctgg ggattgatct acagttcatt tttcggacag gcattaccaa aagaactatt
    26221 tggtatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    26281 tttatccgtg attttcggtc tgatacagat cttggttgga ttattcgtgg cagcaaagga
    26341 aaatatcagg aggaaagatt acttgagtgc tgtcagtgat ggttttgcct ggcaagggat
    26401 cctgattggg atcgttattg cattgattgg tgtcatgttg ataaaaaata cagcatttgt
    26461 ttatcttggc gcggggctat cgatcattgc tgctttatgt atcttaatcg tgccgattat
    26521 ccaatcgccg tctaaagtca agggtgctgc caaaggagcg tataatcttt acggattgac
    26581 tggttatatc ggtgatttag tcagttatac acgtttgatg gcactaggta tttcaggcgg
    26641 aagtattggc gcggcattca atatgctagt tgctttcatg ccgccagccg ctcgattcag
    26701 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgttaag
    26761 tgcctatgtc catggtgcac gattgcagta tgtcgaattt tttggaaaat tttatacagg
    26821 aggcggacgt gcctttgacc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcataa
    26881 gaaaaaaaac aataaataat tggaggattt ttatacaatg atggattact taattagtca
    26941 aaatggtgga atggtatttg cagtattagc gatggcaaca gcgacgattt tttcagggat
    27001 tggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcgttaa caacaagtca
    27061 acccgaaaaa ttcggacagg ctttgattct gcaattgctt ccaggtacgc aagggttgta
    27121 tggattcgtt atcgcctttt tgatttttat caacttagga aatgatatgt cagttgtaca
    27181 aggattgaac tttctaggcg cttctctgcc gattgctttt acaggtttgt tttcaggtat
    27241 cgcccaaggg aaagttgcag cagcaggtat ccaaatttta gcgaaaaaac ctgaacatgc
    27301 tacaaaagga atcatttttg ctgcgatggt tgaaacatat gcgattttag gtttcgttat
    27361 ttccttccta ttagtattga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    27421 gaaaaaatca tctctcagat gaatgaagca gcagagcaag aaagagcggc gttagagcaa
    27481 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaaat cgaaacagaa
    27541 catcaaaaac aaaaagaaaa acaaatcgaa ttacttgaaa aaaaataccg tcagcttagg
    27601 aatcgccaac aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttacga
    27661 cgattgtttg cagatgcaat agctgaaatg gagaattggg atgaatctga acaaatccag
    27721 tttatcaaaa attcattgta ttcattacct ttgacaggca aagtaacatt cattgctgga
    27781 gaaaaatcag cagcttatct ttctcagaca ttgctggatg aatggaacaa tgaacttccc
    27841 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    27901 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    27961 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    28021 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    28081 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    28141 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    28201 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    28261 cgatgcgcta tactttccat aatctgaagg ttttgacaaa agccgagatg acaaatcaaa
    28321 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    28381 ttcatacgca agtttcaaat gaactccctg aaagcattat ggacagtata cgagaagtac
    28441 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    28501 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    28561 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    28621 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    28681 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    28741 atgctgattt tctgcgccct gtcatccgtg agggaaatct tgatctcgta aagcttgaac
    28801 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    28861 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    28921 tggttatcgg caaaagaagc catttcgaac cagaaacaat cagaaagcgg gtgagacaag
    28981 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    29041 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    29101 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    29161 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    29221 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    29281 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    29341 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    29401 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    29461 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    29521 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    29581 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    29641 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    29701 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    29761 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    29821 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    29881 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    29941 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    30001 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    30061 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    30121 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    30181 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    30241 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    30301 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    30361 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    30421 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    30481 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    30541 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    30601 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    30661 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    30721 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    30781 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    30841 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    30901 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    30961 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    31021 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    31081 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    31141 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    31201 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    31261 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    31321 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    31381 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    31441 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    31501 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    31561 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    31621 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    31681 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    31741 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    31801 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    31861 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    31921 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    31981 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    32041 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    32101 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    32161 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    32221 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    32281 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    32341 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    32401 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    32461 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    32521 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    32581 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    32641 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    32701 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    32761 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    32821 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    32881 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    32941 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    33001 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    33061 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    33121 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    33181 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    33241 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    33301 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    33361 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    33421 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    33481 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    33541 aaattggcct ttgttatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    33601 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    33661 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    33721 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    33781 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    33841 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    33901 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    33961 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    34021 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    34081 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    34141 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    34201 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    34261 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    34321 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    34381 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    34441 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    34501 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    34561 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    34621 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    34681 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    34741 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgcacgtgta
    34801 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    34861 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    34921 ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta
    34981 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    35041 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    35101 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    35161 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    35221 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    35281 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    35341 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    35401 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    35461 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    35521 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    35581 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    35641 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    35701 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    35761 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    35821 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    35881 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    35941 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    36001 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    36061 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    36121 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    36181 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    36241 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    36301 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    36361 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    36421 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    36481 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    36541 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    36601 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    36661 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    36721 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    36781 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    36841 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    36901 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    36961 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    37021 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    37081 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    37141 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    37201 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    37261 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    37321 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    37381 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    37441 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    37501 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    37561 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    37621 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    37681 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    37741 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    37801 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    37861 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    37921 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    37981 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    38041 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    38101 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    38161 ggtaatcctt ttaagtcagt aatctcaaga ccggcagctt tatgttgctc gtgtaatttt
    38221 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    38281 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    38341 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    38401 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    38461 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    38521 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    38581 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    38641 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    38701 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    38761 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    38821 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    38881 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    38941 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    39001 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    39061 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    39121 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    39181 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    39241 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    39301 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    39361 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    39421 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    39481 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    39541 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccatcac
    39601 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    39661 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    39721 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    39781 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    39841 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    39901 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    39961 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    40021 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    40081 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    40141 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    40201 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    40261 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    40321 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    40381 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    40441 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    40501 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    40561 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    40621 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    40681 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    40741 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    40801 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    40861 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    40921 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    40981 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    41041 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    41101 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    41161 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    41221 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    41281 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    41341 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    41401 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    41461 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    41521 cttcttttaa agcagattga taaaatcgct tcatgacttg cccgcgtttt tcctgattac
    41581 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    41641 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    41701 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    41761 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    41821 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    41881 aacctgcatc tttcagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    41941 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    42001 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    42061 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    42121 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    42181 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    42241 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    42301 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    42361 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    42421 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    42481 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    42541 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    42601 cacaggaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    42661 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    42721 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    42781 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    42841 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    42901 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    42961 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    43021 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    43081 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    43141 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    43201 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    43261 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    43321 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    43381 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    43441 ggaggtgtga cacgat
//
LOCUS       DAZBTH010000014        41166 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000014 DAZBTH010000000
VERSION     DAZBTH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.014"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACHGNW_000736"
     CDS             complement(451..1233)
                     /locus_tag="ACHGNW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925075.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACHGNW_000737"
     CDS             complement(1298..2134)
                     /locus_tag="ACHGNW_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6925076.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACHGNW_000738"
     CDS             complement(2152..3561)
                     /locus_tag="ACHGNW_000738"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6925077.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACHGNW_000739"
     CDS             complement(3575..5398)
                     /locus_tag="ACHGNW_000739"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6925078.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="ACHGNW_000740"
     CDS             complement(6241..6567)
                     /locus_tag="ACHGNW_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925079.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="ACHGNW_000741"
     CDS             complement(6613..6975)
                     /locus_tag="ACHGNW_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN6925080.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="ACHGNW_000742"
     CDS             complement(7012..7227)
                     /locus_tag="ACHGNW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6925081.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="ACHGNW_000743"
     CDS             7354..7689
                     /locus_tag="ACHGNW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6925082.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="ACHGNW_000744"
     CDS             complement(7765..9762)
                     /locus_tag="ACHGNW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925083.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="ACHGNW_000745"
     CDS             complement(9860..10135)
                     /locus_tag="ACHGNW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925084.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10368..11102
                     /locus_tag="ACHGNW_000746"
     CDS             10368..11102
                     /locus_tag="ACHGNW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6925085.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11141..12283
                     /locus_tag="ACHGNW_000747"
     CDS             11141..12283
                     /locus_tag="ACHGNW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925086.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12378..13769)
                     /gene="sufB"
                     /locus_tag="ACHGNW_000748"
     CDS             complement(12378..13769)
                     /gene="sufB"
                     /locus_tag="ACHGNW_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN6925087.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13774..14244)
                     /gene="sufU"
                     /locus_tag="ACHGNW_000749"
     CDS             complement(13774..14244)
                     /gene="sufU"
                     /locus_tag="ACHGNW_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN6925088.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14231..15466)
                     /locus_tag="ACHGNW_000750"
     CDS             complement(14231..15466)
                     /locus_tag="ACHGNW_000750"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN6925089.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15463..16749)
                     /gene="sufD"
                     /locus_tag="ACHGNW_000751"
     CDS             complement(15463..16749)
                     /gene="sufD"
                     /locus_tag="ACHGNW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN6925090.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16762..17535)
                     /gene="sufC"
                     /locus_tag="ACHGNW_000752"
     CDS             complement(16762..17535)
                     /gene="sufC"
                     /locus_tag="ACHGNW_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN6925091.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17732..18580)
                     /locus_tag="ACHGNW_000753"
     CDS             complement(17732..18580)
                     /locus_tag="ACHGNW_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6925092.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18604..19290)
                     /locus_tag="ACHGNW_000754"
     CDS             complement(18604..19290)
                     /locus_tag="ACHGNW_000754"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6925093.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19283..20317)
                     /locus_tag="ACHGNW_000755"
     CDS             complement(19283..20317)
                     /locus_tag="ACHGNW_000755"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6925094.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20380..20636)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20712..21050)
                     /locus_tag="ACHGNW_000756"
     CDS             complement(20712..21050)
                     /locus_tag="ACHGNW_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN6925095.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21052..21408)
                     /locus_tag="ACHGNW_000757"
     CDS             complement(21052..21408)
                     /locus_tag="ACHGNW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN6925096.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21414..22601)
                     /locus_tag="ACHGNW_000758"
     CDS             complement(21414..22601)
                     /locus_tag="ACHGNW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6925097.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22792..23637)
                     /locus_tag="ACHGNW_000759"
     CDS             complement(22792..23637)
                     /locus_tag="ACHGNW_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6925098.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23871..24836
                     /locus_tag="ACHGNW_000760"
     CDS             23871..24836
                     /locus_tag="ACHGNW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN6925099.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25213..26256
                     /locus_tag="ACHGNW_000761"
     CDS             25213..26256
                     /locus_tag="ACHGNW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN6925100.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26318..26782)
                     /locus_tag="ACHGNW_000762"
     CDS             complement(26318..26782)
                     /locus_tag="ACHGNW_000762"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN6925101.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27028..27975
                     /locus_tag="ACHGNW_000763"
     CDS             27028..27975
                     /locus_tag="ACHGNW_000763"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6925102.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28186..30258)
                     /locus_tag="ACHGNW_000764"
     CDS             complement(28186..30258)
                     /locus_tag="ACHGNW_000764"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6925103.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30191..30568)
                     /locus_tag="ACHGNW_000765"
     CDS             complement(30191..30568)
                     /locus_tag="ACHGNW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN6925104.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30697..31257)
                     /locus_tag="ACHGNW_000766"
     CDS             complement(30697..31257)
                     /locus_tag="ACHGNW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6925105.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31407..32699
                     /locus_tag="ACHGNW_000767"
     CDS             31407..32699
                     /locus_tag="ACHGNW_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6925106.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32759..33877)
                     /locus_tag="ACHGNW_000768"
     CDS             complement(32759..33877)
                     /locus_tag="ACHGNW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN6925107.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33891..35312)
                     /gene="arcD"
                     /locus_tag="ACHGNW_000769"
     CDS             complement(33891..35312)
                     /gene="arcD"
                     /locus_tag="ACHGNW_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN6925108.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35558..36679)
                     /gene="mnmA"
                     /locus_tag="ACHGNW_000770"
     CDS             complement(35558..36679)
                     /gene="mnmA"
                     /locus_tag="ACHGNW_000770"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN6925109.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37083..37682
                     /locus_tag="ACHGNW_000771"
     CDS             37083..37682
                     /locus_tag="ACHGNW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6925110.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37820..39106)
                     /gene="efmA"
                     /locus_tag="ACHGNW_000772"
     CDS             complement(37820..39106)
                     /gene="efmA"
                     /locus_tag="ACHGNW_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN6925111.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39377..39721)
                     /locus_tag="ACHGNW_000773"
     CDS             complement(39377..39721)
                     /locus_tag="ACHGNW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN6925112.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39858..41003)
                     /locus_tag="ACHGNW_000774"
     CDS             complement(39858..41003)
                     /locus_tag="ACHGNW_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6925113.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13473 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
    10381 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
    10441 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
    10501 acaaaaaaac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
    10561 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
    10621 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
    10681 cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag
    10741 atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca
    10801 agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag
    10861 aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc ctcactagca
    10921 gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca
    10981 tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa
    11041 cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct
    11101 agtggtaagt actccttgta aactaagagg agatgattta atggcaaaaa tatcaaagta
    11161 caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg
    11221 aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa
    11281 agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat
    11341 gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc
    11401 aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct
    11461 taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa
    11521 atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc
    11581 aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta
    11641 tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa
    11701 agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag
    11761 aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca
    11821 tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac
    11881 aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg
    11941 gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca
    12001 acatgttttt acaacagtta aaaacactct atacattcca aacactgtta atgattggtt
    12061 aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac
    12121 acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg
    12181 gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa
    12241 gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc
    12301 tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc
    12361 tagctcctat aacaggttta cccaacactg ccttccatct cataactgat caatcgattg
    12421 agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg
    12481 atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct
    12541 tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca
    12601 ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata
    12661 tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct
    12721 ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg
    12781 atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg
    12841 gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca
    12901 cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt
    12961 actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct
    13021 tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc
    13081 gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg
    13141 aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg
    13201 acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct
    13261 aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat
    13321 tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc
    13381 atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt
    13441 tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc
    13501 cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg
    13561 tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc
    13621 agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact
    13681 tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat
    13741 ttatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg
    13801 gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc
    13861 acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg
    13921 gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc
    13981 tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact
    14041 gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac
    14101 ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc
    14161 agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa
    14221 attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa
    14281 ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg
    14341 atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc
    14401 cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa
    14461 cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt
    14521 gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac
    14581 cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg
    14641 tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat
    14701 cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat
    14761 ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac
    14821 agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga
    14881 tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta
    14941 agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg
    15001 catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg
    15061 ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg taagagagca
    15121 ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc
    15181 ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc
    15241 gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac
    15301 cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt
    15361 ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt
    15421 gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc
    15481 ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga
    15541 accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat
    15601 cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac
    15661 ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat
    15721 caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat
    15781 accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat
    15841 cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct
    15901 gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc
    15961 agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa
    16021 aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat ctacggctga
    16081 atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt
    16141 tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc
    16201 tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa
    16261 ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt
    16321 cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat
    16381 ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat
    16441 gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg
    16501 gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat
    16561 ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg
    16621 cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc
    16681 aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt
    16741 ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt
    16801 actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat
    16861 gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg
    16921 ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt
    16981 agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa
    17041 tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa
    17101 accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc
    17161 atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt
    17221 gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat
    17281 agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc
    17341 gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga
    17401 taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat
    17461 tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat
    17521 ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc attttagact
    17581 aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt
    17641 aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca
    17701 gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa
    17761 cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa
    17821 tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt
    17881 aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt
    17941 aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta
    18001 aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat
    18061 cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta
    18121 gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat
    18181 cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga
    18241 cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa
    18301 tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat
    18361 agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat
    18421 gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg
    18481 ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca
    18541 gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc
    18601 ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac
    18661 gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc
    18721 ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa
    18781 tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg
    18841 tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag
    18901 tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc
    18961 ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt
    19021 agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact
    19081 gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga
    19141 gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt
    19201 catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc
    19261 tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt
    19321 ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca
    19381 atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga
    19441 tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg
    19501 tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg
    19561 aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt
    19621 gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga
    19681 ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat
    19741 tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca
    19801 atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga
    19861 taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt
    19921 ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc
    19981 cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga
    20041 aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt
    20101 ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca
    20161 ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc
    20221 ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga
    20281 atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct ttctcttatg
    20341 atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac
    20401 ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg
    20461 acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt
    20521 acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc
    20581 aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt
    20641 catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg
    20701 attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc
    20761 cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta
    20821 acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca
    20881 acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac
    20941 gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca
    21001 ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag
    21061 ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat
    21121 cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt
    21181 aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa
    21241 caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg
    21301 tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt
    21361 ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc
    21421 cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg
    21481 atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt
    21541 aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg
    21601 atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc
    21661 atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag
    21721 ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt
    21781 acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc
    21841 tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac
    21901 tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag
    21961 aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa
    22021 gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca
    22081 aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca
    22141 atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg
    22201 atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac
    22261 gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat
    22321 ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag
    22381 atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag
    22441 acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct
    22501 acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa
    22561 tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa
    22621 aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac
    22681 atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc
    22741 tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct
    22801 gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc
    22861 aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg
    22921 gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg
    22981 accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt
    23041 atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc
    23101 ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat
    23161 caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga
    23221 ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg
    23281 cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa
    23341 ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg
    23401 ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg
    23461 cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg
    23521 ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc
    23581 tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc
    23641 accacttcca taacttttta tgataattac tataaaaact atttgattta aatatacaaa
    23701 aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat
    23761 gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat
    23821 tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact
    23881 ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa
    23941 atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt atctctatga
    24001 ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt
    24061 tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt
    24121 gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg
    24181 ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg
    24241 atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca
    24301 tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat
    24361 tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata
    24421 tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa
    24481 caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt
    24541 tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt
    24601 tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta
    24661 ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag
    24721 ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt
    24781 tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat
    24841 cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa
    24901 acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca
    24961 ccgtttacca agaggtcgta aaagaagtgg tcagcttcaa gaaataagaa ggaatttacg
    25021 aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga
    25081 acaccgttta ttcgaaacaa gaggtcgtac aagacttttg tcacgacctc ttgttctttt
    25141 gtcaaaatac tggtttagtg ctactatgtt cgagtatgct tttagtattt attgacgaaa
    25201 ggaaccgatc aaatgaaatc attgtcacat ccaataaaat ctgtaaagga ttcagcggtc
    25261 cgcgaaatct tacccggttt actgctttct tttgccatcg ctgccttcag caggcttctt
    25321 gctatttatc tgccacagtt aggtggagcg actattgcta ttttattggg tattttatta
    25381 ggaaatactt tttttaaaca gcctgttcta gcaatcggca caaaatttgc tgaaagcaaa
    25441 ctattagaat tctctgtttt tttactaggt gcaactgtca cagctcaaac gattgctcaa
    25501 attggtgtaa aaggaattgt ttttgtgtta ctgcaaatgt cagtcactat catcggtgct
    25561 tacttgattg gtaaaaaact gcaattcagc gatacttctt ctttgatgat ggctggagga
    25621 aacgctgttt gtggttcttc tgctattgct tcaatcgccc cttccatcca tgcgaaagag
    25681 gaagaaaaag gacagatcat cacactagtc aatctactgg gtactgtgat gatgttgact
    25741 ctcccttttc ttggattaca gttgtttggt ggaaatctca ttgaaaaaag tgcacttttg
    25801 ggtggtacac tccaatctgt tggtcaagta gtcgctgggg caagcttact tgacaattct
    25861 gttgtcgaat tcgccatgct atttaagatt ctacgtatta tgatgctcgt tgttgttgtc
    25921 tatctgtttg aaaaattcat gcacaagaaa caaccagctc tgtttgaaac gccaactgat
    25981 acgcagaaaa aacgaaaatt accgcttcct tggtatgtat tagggtttgt gatcttttgt
    26041 ttggtcaaca gcttgttttc tttacctgta gttattgacc aatcggcaca ttttatcagc
    26101 agctggtttg agatcactgc tttagcggca attggtctgc gtttagactt tgcaaaattc
    26161 atgaaagaag gtacacgctt cttatcttat ggattagctg tcgggcttct ccaaacagtg
    26221 atggctttac tatttatttg gctgatcaaa gtttagaatg aacatgaaaa aacagttgtg
    26281 attctcgcat ggaacaatgt ccgaatcaca actgttttta attttgcaga tatccttcta
    26341 acaacaacaa ttggcgtttg atttcaaccc catctattgc gtttcccata tatcctgtca
    26401 gttttccatt ttgcccgatc actcgatgac aaggaataac gattggcaat ggattggttc
    26461 gatttgcctg cccaatcgct cgcacagctt tcggactatc aacagcaacg gctacttctt
    26521 tatacgttcg gatttctcca taaggaatgg tcgacaaagc tttccagaca ttttgctgaa
    26581 aaggcgtacc tctctttata gataaaggaa catcaaattc ttttcttttt cccgcaaaat
    26641 attcttcaaa ctgttcttgt gtgcgcgtta aaattcgctg attcgctgat tcgctttctg
    26701 aaattggctg aaaagataca gcagtcaatc cggattcatc tgcatctaac cataaaaagc
    26761 cgataggtgt gtgtattttc attttgtttg ctcctagtca atagactgga acttcctcct
    26821 ttttctctat ccaaaactac ttgccaacta ataaaaaatg ttcttaaaag tttgataaac
    26881 cgaggaaaca tggtaaacta aatacgttag tttacaaaaa gaatacctgt tcttttacgt
    26941 aaaatcaaat tttaaaggtg ttatttaagg tttattaaat tttgaatgta aaccatatat
    27001 aaacatacga ttgaaaggat agaaacgatg aaagtatcaa ttgttgtccc atgttttaat
    27061 gaagaaaaaa ctgttcctct tttttttgat gaggttgaaa aattcaaagg tgcccatgag
    27121 ttcgaatatt tgtttattga cgacggatca aaagacggta cgttagcagc aatcaagaag
    27181 ctagcctcag cttatcctta tgtccgcttt gtctcatttt ccagaaattt tgggaaagaa
    27241 gcggctctgt acgctggatt gcaagcttct acaggagatt tagtgaccgt gatggatgtt
    27301 gatctgcaag atccgcccga actgcttcct gagatgatca accggatcac tacttctgat
    27361 atcgattgtg tcggcacccg gcgttcgact cgtgaaggcg agcctgcgat acgcagtttc
    27421 tttgcgaaaa aattctacca gttgatcaat aaaatcagcg atacagaaat ggtcgatggc
    27481 gctcgtgatt tccgcttgat gactcgtcaa atggtggatg ccgtcctaga attaacagaa
    27541 tacaaccgat tttcaaaagg attatttagc tgggtcggat ttaaaacaga atatatttct
    27601 tatgaaaacc gcgaacgtgt ggctggcgaa acatcttggt ccttctggaa attattcaac
    27661 tactcgattg atggtatcgt gaacttctcg gatgcaccgt taacgattgc ttcatttatc
    27721 ggcgctttgt cttgtgtcgg ttctggtatt gcaattttgt tcattatttt gcgggcattg
    27781 ttgtttggtg atcctacatc aggatggcct tccatggtct cgatcttcct gttcattggt
    27841 ggaatccaat tgttatgtct tggaatcatc gggaaatata ttgggaaaat cttcttagaa
    27901 acgaaacagc gtcctgttta tatcataaga gaaacagaaa aagtgaagcc aaatgaattg
    27961 aagcctcacc aataatatac gtcaaatgaa gtaaacattt ttttaaatga aaaaggactg
    28021 tgacaaaagt cttgtcacag tccttttttc cgaataaatg gtgttccaga agtagcttct
    28081 tcggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    28141 tttcttgaag ctgaccactt ctatcacaac ctctttcata tcttattact tcttcattgc
    28201 tgcttttagt gctgctgcta atgggctttc ttctggttct tctgcttgtg catatttttt
    28261 cagcaagcgt cgttcttcat gtttggtcat tttcttcttc tgctcttttt tatcaagcaa
    28321 tttttctgtc gttccatcgt atttacaacg gaaataagca ccgtttttac cttcgatgat
    28381 ttccattttc cggtgacatt gcgggcatcg atgattagag acttttggat cttttcttct
    28441 gcggtagttg caatctgaat tggtgcagac atagattttg ccgtcacgtg tgtttttctc
    28501 gcgcagattg gccccacatt ctgggcattt cttttgggta atcgagaaat cttgatattc
    28561 ttgtttgctt gatttgatct cattcaccaa gcgttttgtt tctttttcga ttccattcaa
    28621 gaaggttcct gcttgtttct ttcctgcagc aatagcttca aggtcttttt cccagctttc
    28681 tgtcaaatct ggtgttacta aagagggatt taccaatact aataattgct tacctttagg
    28741 agatacttgc agtccttgag gggtacgttc catcagttca gatttgatta gtttctcgat
    28801 aatctctgca cgcgtagctg gggttcccag actgtatttt tccatttgac caagcaagct
    28861 tgcttctgac aatggtttag gcggtgcaga tagttctttt ttgatggcga aatcgggatt
    28921 gattctcatt cctttttccc atttgactgt ttccgtctca gtttcagttt cgcctttcca
    28981 tcctgcttgg acgacacgat tttgccgaaa aatgaatcgt tctttaccaa attcaaccgt
    29041 aacttttgtt tggctgactt tatggggttg agcaaacagt cctaaaaaac gagagacaat
    29101 catttgatag atttttcgct cttcattact taatttttca taacgtgctc tgttttcagt
    29161 tggcaaaagt gcatggtgat cggtcacttt actgtcttgg aatacttttg tctgttggac
    29221 gatggctccg tttttcaaat actgcttgac ctcaggagca aagtcgctga ctgcttgcaa
    29281 tcgttctttc atcgtacttt tcatgtcatt tgtcagatat ttgctgtctg tacgcggata
    29341 agtaacgatc ttatgggtct catacaaact ttgtacaaga cttaatgttt tctttgcaga
    29401 atagccatag cgttgattgg cttctctttg gatctctgtt aaatcataag gcaatggcgc
    29461 agattccgtt tttaattttt cttggacttc tgtgacagtt cctttgcttg tactgatcaa
    29521 ttgaacaagt ttttgtgctt cttcttgtgt ttttaaagca aattgatttt tttgaagcat
    29581 tttggcttgt tcttttccag cttgcaacgt gatcgtgaaa tacgtttgtg gacggaactg
    29641 ctcgattttc ttttcttggt ctctcactaa tgctagtgtc ggtgtctgca cacggcctgc
    29701 tgataaatta tccgcgtact tcacagttaa ggctctggtc acattcaatc ctaccagcca
    29761 gtccgctttt gcccgggcaa gagcagattc atacaatgca tcatattctt tcgcaggtct
    29821 tagttgcttg aatccagttt tgattgcttt atctgtttga gaagagatcc ataaccgctt
    29881 cactggttta ttgaaatgca catactctaa gatccagcga gcaacaagtt ccccttctct
    29941 tccggcatct gtcgcaatga ctgcttctga gacatctttt cgctgagcca gctgcttgat
    30001 tgcttttaat tgatgtcctg ttttaggaag tggtttgatt cctagttttt tagggatcat
    30061 tggcaacgtg tccatttgcc agctctgcca ttccttattc agatcttccg gcattttcaa
    30121 tcctaataaa tgaccaaggg cccaagtaac gatcaccttg ggtccttcat aataattttt
    30181 gtgcttttga ttagcaccaa gcaccttgct taggtctttc gcaacgcttg gtttttccgc
    30241 tagaattagt tgtttcataa aattcctttc tgctttctac tgtgatgggc tgatgatttt
    30301 tcaacaacgc aagtcccttg tattgctgta aatcatcgtc acactcttca caccagcgct
    30361 catctccgtc attccaaaag gcgctgagaa agttactttt tgcattgata tattcataat
    30421 tttgatggat ttcgttccat tgtttttcgt acgcttgtcg tgcttcttcg aaagactcga
    30481 atgtttcttc ttcttcaata tcttcttccc agtcttcaaa aaaccaccaa ggctcattgt
    30541 ccccgaacat ggtaattact tgatacatac tcgtgctcct tctttcagct tttctagact
    30601 catcttagtc acttttcaag aaagttgcaa gaattttctc ttataaaagg gtcatttaat
    30661 gaacatctta aaattgttac aaatgaatct gattgtttaa ggcaaagtag cgataaagtt
    30721 attgatcatc caaaaatagc tgtcttctgg atcgattgtt ggttcttttg taaagtatcc
    30781 taaaaaacgt aaggaaagat aaccgtggat catactacga agttccctgc ttcgatgaac
    30841 tttttcttct ttcgttaatt gaaaagcctc aaatatctgc aaaatgatct gattcgtttc
    30901 atggattcca tctaataaaa cagcttcatt ggtatgcggt acactgatca gtaactcata
    30961 cactgcttga tttgaaaaag caaaggatcg ataagtttct gcatagatct gcagtgcttc
    31021 ctgaccactt tttcccacaa gagcccgtcg cagctggtca ttaagttcct gcagaagttt
    31081 cgctgttagc gctgttttga catcacgaat atttttgaaa tggttataca gcgagggagc
    31141 cttgatatcc aagcgtttgg ctaaatgctg aaaggttatt tgctgaacat ccatctcttc
    31201 tgctagttct cgaaagcagt cgatgatttt ctcttgagac agatttcttt ttttcatttc
    31261 catgtttcct ctcataaaat aaatccttca ctctttcagt ttaccacgaa aaagaaagag
    31321 aaaaaagaga ctttcattga taaactacat attgtagttt ataataaaga gctgtagatg
    31381 aaaattttca gaaagaagga gtctaattgg ctgcaagaat aaaggaaaaa ttatttagga
    31441 aaagagaaac ttgggaacaa aacttgtttg tcttatggtt tggaacattt atggctggta
    31501 tcggatttag tttagtcatg ccttttatgt cactttacat agatacgtta ggtaattata
    31561 gtacgtcaca attgaacttt tggagcggat tgactttttc atctactttc ttggtgacta
    31621 cgcttatctc tccttggtgg ggaaaattag ccgaccaaaa aggaagaaaa ttgatgctgt
    31681 tgcgtgcttc attaggaatg gcaatcgtca tttctttgat gggttgcgtg actagtgtgt
    31741 atcaattagt cgcattgcgt ctgcttcaag ggattttttc tggttatatc agtaatgcca
    31801 ctgcccttgt cgcaactggg acgccaaaag aaaaaagtgg tcaagttctt ggaaccttag
    31861 caactggttc tgttacagga acattacttg gaccgcttct tggcgggatc tctgcttcag
    31921 cgtttggcta tcgccctacc tttttcatta cagggatcat cttatttttc gtcttcttgt
    31981 tgagtctgtt tttcgtccat gaaaaatttg tcccagtgaa aaaagaaaac atggtttctg
    32041 ccaaacagat tttcaaagac ttgaaatatc cacacgtcgt catcggtatg ttcgtcacaa
    32101 cgatgatcat ccaagcatcc aacaattcca ttagtccaat catcagtctg tacattcgcc
    32161 aattgctgca tggacatggg aatgttacgt tgatcagcgg aatcattgct tcgatacctg
    32221 ggattgccac tttgatagcc gctccaagat ttggtcgctt aggtgacaag attgggagtg
    32281 aaagaatact tgcgattggt ttgggttttg cgattctggt ttatatccca atggcattcg
    32341 tcacgaatgt ttggcaatta gctgccttac gttttttgat tggtatctct gatgcttgtc
    32401 tgctccctgc tgtccaagca ttgatcacaa aatattcgcc tcatcaagct gctggacgga
    32461 tcttcagtta taaccaatct ttccaagcaa ctggtaacgt agtagggcct atgatcggct
    32521 ccagtgtttc cagtgcattt ggttatcgtg gcgtctttct ttcgacttct cttcttgttt
    32581 tggtcaactt cttgcttgta cgacacaata cacaagagat ccatcagcaa gaaactgcag
    32641 aagaaaaagc ttcaggagtc tccatagctt cagggcactc tccttctcat agacaataag
    32701 cgtaaatgaa aaacactgct tgaacagttg tcacagactg tccttgcagt gtttttgttt
    32761 aggcctgttt tttcaagaac tcagataaga ggaataatcc cttttccaag gtttcttttt
    32821 tacaacagta acctagtcgg gcatgcttcg gtaaatcaaa ggcacttcct ggtactaata
    32881 gtactcctgt ttcttctaat aaattgatac aaaatgtctg gtcatcttct tcgatggcta
    32941 atttaatgaa agaagtagag acgtaattgg gagcgacaag ttcaactttc ggttcattgg
    33001 ctacccattg ttttaaaatc acaaggttct ctgtaatgat ctttttgttt cgcgccaata
    33061 ttttttcttt attcttcaat gcatgtacag caagatcatc tgacaggact ccgccgcaaa
    33121 tcatcgtgta gtctcgatac tttcgaaaaa cttctgccaa ctcaggtcca gtagctgtcc
    33181 agccaatacg aatacctgga atggagtagg ttttggatag ggaattggtt gctatacctt
    33241 tttcatagac atctacgatc gacaagaatt cacctttatc cgttaaaggc gcatatactt
    33301 catctatcaa tacatatgca tctactgtac gagcaatttc tgcgattttt tctaaagttt
    33361 tccgatctag caatgtgcct gtaggattgt ttgcactatt caaacagatg atttttgttt
    33421 caggtttgac taaggcttct agctgttcta aatcaaactg ccaatcttct tcttccttca
    33481 aatgaacaaa gtcaacagcg gcacctaaag atttggggat gtcatataat tgctgataag
    33541 taggaagcat cgaaacaaca tggtctcctg cttccactaa cgcgtataga gctaaaagat
    33601 tagcaccagt cgctccattc gtttgtaaga tgttttccgg atcgacggtt tgataaagag
    33661 aagcgacttc ttgtttgaaa acatctgatc cttcgatcca gccatagtcc aatggttcgt
    33721 ttttgcgttt ttcgaaaaat ttacttacgc tagtcccatc taagccaacg agttcgtcta
    33781 atgtcaatgc ttcgatcgag ctttgtgcaa tatcatatac cgctttcttt tcccatttat
    33841 ttagccattc ttctacaccg aaatttgcga ttttcatgtt tctttccccc ttatatcttg
    33901 atccagccta cagcaagcaa cccgattgaa agcagtgcgg ccacacaaat cagcccaatc
    33961 attacttttt cttttcccgt aatttgatgt ttggtttcac gacaagcgat ccagtagaag
    34021 caaaaaccag gaagataggc aatactacat aaaaggacat aagaaaatcc tgctaaaatg
    34081 atgcccatca attcaaaaac tacagcaatc ccaccgatca ctacttgttt tttgattcct
    34141 tcttttaaag aaaactggac ttgatagatc ccgacaagta gataacaaat caaaattgcc
    34201 gctgtacata aagaataggc aaattgatag gcataatcag taactaagaa agtcaataag
    34261 aatagattag tcaagattgc agtaacaact aacgaataag ttggggcatt ttttttattc
    34321 gtttttcccc aacctttcgg caacagaccg tctcgagcca tcaacatcgt tgtttctgct
    34381 gggagcatcg tccaagacaa ccaagaacca atgatcgaaa taatcaagcc gatattgatc
    34441 agcgctgctc cccaatttcc cacgattcct tttaaaatat ttgccatagc aggttgtgaa
    34501 atagttgcta actcttcttg agataagacg ccgtatggca agatcgaagc aaggatatag
    34561 ataaacaata aacaaagcag accaatgatc gatgcttgct gggcttctga acgtttcttc
    34621 gcgcggttgg ctaaaacact tgcgccttcg atccctacaa agacccacat cataaccatc
    34681 aaacaacctt taatctgcaa ccagatcgtt ccttgctgcc ctgttccatt cactagatta
    34741 tcagatacgt taccccaaaa atcagcagtg aatatattca ctttaaatgc gataaataca
    34801 aatattaaaa ataggaataa tggcactaat ttacagatcg tcacgattgt attgatgacg
    34861 ctcgctcctt caactccatt gtttactaat aaagtcagac cccaaataaa aatacttgca
    34921 aataaaatag aaggaacatt ttgtccacct ttgaatatcg ggaaaaattc tcccatggcg
    34981 ctcatcagca tcgttgcaaa agcaacattt cccagccacg cagacaacca gtaaccccaa
    35041 ccactgatga agccacctaa tttaccaaat gaagcttccg cgtaaccgaa aataccacca
    35101 tctagatctg gacgcttttc tcctaaatga ttcagcgata ataccaaagc taaaatacca
    35161 atcccaacga ttatccatga aaggatcgct ggccctggag ctgcagaact agccagatca
    35221 cttgtgatcc caaacacacc gcttccgatt gacgaactga caaccagagc aaccagtccc
    35281 catttgctaa tacccgtcga tttcttttcc atttccatca ccctcactta taaaattgaa
    35341 caaatcactt gcaaaagatt cccttctatt ttgcaactgc tcttgcttaa caaaaagcaa
    35401 ctttattcat attattggat attatgaact ttttacacaa gtatttcaag tataaaatga
    35461 ataaaaatta attttattgc aaaaaaacag ctatgctatc caaaagaaaa ggtgtttctt
    35521 ttgttagcca tagctgtttt gattcattta atatcgctta aacgtattgt aaaacttttg
    35581 tttcttgata tgcgtggtcg atcaaaccac cacccagaca ttccattcca tcataaaata
    35641 caactgcttg tccaggagtg attgcacgta caggttcgtc gaatacgact tctgcacgat
    35701 tatcatccaa taagcgaact gtcacaggaa cgtcttgctg acgataacgg aatttcgctg
    35761 tacacttgaa ttcttttggc attggttcat tcgtcgtaaa gtggatttca cttgcatcca
    35821 agcttgtggc atacaatgca ggatgatgga acccttgccc cacatataat gtgtttgtcg
    35881 ctagatcttt tcctacaaca aaccagggtt cttgcgaatc tcctccgcca ccgatcccta
    35941 gtccttgacg ttgaccaatc gtataataca tcaagccagc atgttgtcct ttgacttcac
    36001 catcaagagt gaccatattt cctttttttg ctggcagata gttgctcaaa aattgtttga
    36061 agttcttttc accaataaag cagattcccg ttgaatcttt tttcttagca gtcgcaaggc
    36121 ctgcgcgttc agcaattgcc cgtacttctg atttttccat gccgcctaga gggaacatcg
    36181 tttttgaaag ctgttcttgt gataattggc taaggaagta agtttgatct ttattattgt
    36241 ctactccacg aagcatgtgg accacgccgt tttcatcgcg agtgacttgc gcataatgtc
    36301 cagtagctac ataatcggcg ccaagatcca ttgcataatc caagaatgct ttgaatttga
    36361 tttctttgtt gcacatcacg tctgggttag gtgtacgtcc tgcgcggtat tcggctagga
    36421 aatattcaaa tacacgatcc caatattctt tttcaaaatt cacagagtaa taaggaatcc
    36481 cgatttgagc agctactttc gctacatcct tataatcttc cgtggccgtg catacaccgt
    36541 tttcgtccgt gtcatcccaa tttttcatga atattccgat cacgtcgtat ccttgttcct
    36601 ttaacaaaag tgctgttaca gatgagtcga caccgccgct catccctacg acaacgcgtg
    36661 ttttgctgtt gtctgtcatg atatcaccat catttcaatt agattctgaa acatctacga
    36721 gttgaaggtc gcaacttttc agtattttac attatacgtc atatttctga aaatttcatc
    36781 ccttaattat caagtcgtaa aaatactgtt ctactggacc tattcagtac acaaacatga
    36841 tagtcaacca aaaaatcact catcttttga gcattcgata taaaaaattg gcatactcat
    36901 aaaaagggcc agcctttcgg caataaggaa taattttata aattgtgaaa aactattttt
    36961 caaaattgct tcttatcgct taaagctgca cgtctcattt acaacctttt ctttttagat
    37021 gttatgagtt gctcagataa gtgattttgt ttactctaag aaaaaaggga ggcaaaagat
    37081 acatgaatta ctttatcagt gatatgcact ttttccatga acgcttatta ggaaaaaacg
    37141 actttgctcc gcggctcttt gcaaatgtgg atgaaatgaa ccaacagttg atcactagct
    37201 ggaatgcagt aataaaagaa accgaccatg tttatcatct aggcgacctt gctatgcatc
    37261 cgcaatatga aaaagcaaat gcagaaattt tggaactggt cactcaactg caaggaacaa
    37321 tccacttgat caaagggaac catgacagtc gcgctttttt ccgttattta gaaaaaaatg
    37381 atcctaggct tactgacgga agtcccaaat tttattttta tgatgtaggt gccatcgtta
    37441 aattcgacca tcaacaatat tacttgactc attatccgat gttgctcggg cctaacgaca
    37501 ctatccgcaa tcttcatgga catatccatc attacagcgt acctatcgag aacgatgtca
    37561 atgtaggcgt agacgcaccg gaaagagaac tcttaaagga aaaactacca tttggcactc
    37621 cgttatctga gcaacaaatc aaagaaatct atgaagcaaa gaggaagtac gataggtcgt
    37681 gaaaaaactg ccctatatca agtattaaac aaataaagat aattactcat atttttcatt
    37741 tgagtaggct tattgccaca ggaacattag gatcagaaat ggagttgtaa caagacttct
    37801 gtcacaactc cattccatac tattcgtctt tttgctcatg gtgttctttt accagttttt
    37861 tctgtaattc tttatcataa tttcgggcag ctgacgttaa aaataaaacg attccgcata
    37921 ataaaatccc gattccggaa aacaagaaca ttttttctac ccctaaacga tcagctaatg
    37981 gtcctgcaaa taataatcca attggacctg tcaggttcat caaagagttg aagttcccta
    38041 agacacgacc taaaacattc gattcataac tctgctggat cattgccatc agcagtgtat
    38101 tgaaatatgg cgttgcacaa cctgctcctg cattcaagat aagaaagtac aagaaccctt
    38161 gactatcgtt tggaaccaat ccgcttgcac cgatcgtcag gcccataatg atgtaggcta
    38221 taataatagg tttcattcgg tctttccatg ttcctataaa actgatcagt gcaccgccta
    38281 agagcattcc gactgcgtag actacttcca ctgctcccgc ctgtccaacg gaaccaccaa
    38341 agtaatctag cgtcatcaat ggatacagac tgattgctgg catgaacaac agcatcacaa
    38401 atgcaccatt gatcgttatg taccataacc ctttattatc catcaattgc tgcagcccaa
    38461 attttgcatc tttaaaaata gtaagcggaa cttccaatat ttcaggagaa acttttggga
    38521 tcttcacaaa aatcaataag ccgacaccaa aaactgcccc caatacatct aataaaataa
    38581 ggtaattgac aggaacgact gaaaacaaag ccgcacctag agctggcgca atgatgaaat
    38641 tcgctgattg gaccattcct aactgtccat tcgtccttgt tatgtgagaa gatggaacga
    38701 ttgtcggcat aatcgactgg atcgttggca tttggaatgt ttgtgcgacc gaacgcataa
    38761 acaaagacac aaaaactagc caaatcggga aggattctga aatcgttccg actactgcta
    38821 aaataagcgc gaaaatcgct acaataatat ccgtgacaat caaaagagct tttttatccc
    38881 agcgatccac tagcggtcct acaaatgggc ttagtaaaac catgggaatc attcctagta
    38941 aagtagcaaa actcaaaatt gtcgccgaac cggtttctct tgtcaaatac cagatgatcg
    39001 catattggac gaccatacta gtgattcctg acaaaaactg tcccgataaa aagaacaaat
    39061 aatttctctt ccaattcgtt aaaacgaccg actgttcatt ttccataaac atccctcttt
    39121 tctttttcat tttgtatagc gtttgcacaa caatcattat acgggattct tttttcaaaa
    39181 aatacctttt tccctaaata acaatcgttt caatttaaat ttctttactt agaaacaata
    39241 aatcagcaaa atattaaaaa taacaacatg tgttaaattt aaaaataaaa aagacaacta
    39301 atttgcgtcg tccttacaaa gttagtcatc taacaatagg ccgtttcaaa actagttgtc
    39361 tttttatatc cacagatcat tcgacttttt cgaataaccc acggctaatc atcccatcca
    39421 tcagaaaata aaatttatct tggctcattt tctgttttgg atctttgaaa atattgactg
    39481 ctttcaatcc atttgctgta gtgatcgaca ttttcaatgt ttgaatatct tttgatacag
    39541 tgatttttaa tttgaatcct tctttgcttt gcggcgtggc atctaatggt cggatcaatt
    39601 gataattgcc tccgtttgtt tctgtaaatc cattatcgcg caatgtatat ctttttgcat
    39661 caggatgcaa acgatagcta attggtgcgc ccagtaccgt tgctgttgtt tcaaatgcca
    39721 tgattttcac ctcgataaaa ttagtttctc tttattataa aagtttatcc tgatttttac
    39781 taggtttttc agttaagttc cctaaaaaat gaaggcgttc tctcagacat ctgttcatat
    39841 aagaatgact tcactagtta ttttttgaca gcacgaacta attgatcaag gaaatactgg
    39901 atgtcgtctt cggtatttcc caaaccaaag ctgatacgga tggattcttt tagtatcggt
    39961 gtatcttctc catacattgc ttccaatacg tgtgaaggct cgatatttcc tgctgtacaa
    40021 gctgaaccag tagaaatagc gaccccatta agatcaagct tcatcagtag caaatcattt
    40081 gcttcgccaa ttaagcgaag attcaacaca tgagggagtt tattcgttaa atcaccattt
    40141 acttgataag ggatttcgtt tgcttctaat gtatcaagaa tcaattgctg gaatccttta
    40201 tatttgttgt tacgtgcttc acgttcttca ggtgacagca gttcaactgc ttttgccata
    40261 ccccagatcc ctgctaaatt ttctgtacct gcacgccgct tttcttcctg ctccccgcct
    40321 aaaagcaatg cagggatatt tactccgtct cgtttatata agaaaccgac gccttttggc
    40381 ccattgattt tatgagcaga gatacttaat aaatcgattc ctaattcttc tggatggatc
    40441 gtttggtttc cgtacgcttg taccgcatct gtatggaaat aagctggatg atccgctaaa
    40501 agttcaccga tttctttgat tggcatcaaa tttccgattt cattgttccc gtacatcaca
    40561 gagaccaaga tcgtatcttc tcgtaacgct tttttgacct cttcgatcga cagattccct
    40621 ttttcatcta caggcagata tgtaacgtca aacccctttg tctctaaata tttcatggta
    40681 ttaagaacag caggatgttc gatagctgta gtgattagat gtctgccttc tttttgacgc
    40741 gagaacgctg tttctaagat agctgtatta tccccttctg ttcccccact gttaaagatg
    40801 atctcgtgag gttttgcttg cagactgtct gcaatgattt gtcttgctgt ttccaatttt
    40861 tcatgcgcgt ggcgaccaaa tccatgtatg cttgaaggat ttccaaacgt gtcttgcatg
    40921 attgccgtca tcgcttcaat tacttgtgga tgcatggggg tagttgccgc atgatccaga
    40981 taaatagttt tcacttcatt catcccttcc aaattattgc taggcacatt ctagcataaa
    41041 atagcgaaca aaccaaagaa gagctcattt ttgtctagag gacgtttcat gggcatagaa
    41101 aaaaggagct gtgacaaaag tcttgtcgca gctccttgaa gctgctcact tctgtcacaa
    41161 cttctt
//
LOCUS       DAZBTH010000015        39230 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000015 DAZBTH010000000
VERSION     DAZBTH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.015"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..958
                     /locus_tag="ACHGNW_000775"
     CDS             77..958
                     /locus_tag="ACHGNW_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6925114.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            971..2581
                     /locus_tag="ACHGNW_000776"
     CDS             971..2581
                     /locus_tag="ACHGNW_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925115.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2658..3317)
                     /locus_tag="ACHGNW_000777"
     CDS             complement(2658..3317)
                     /locus_tag="ACHGNW_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6925116.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3600..4265)
                     /locus_tag="ACHGNW_000778"
     CDS             complement(3600..4265)
                     /locus_tag="ACHGNW_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN6925117.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4412..5017)
                     /gene="dhaL"
                     /locus_tag="ACHGNW_000779"
     CDS             complement(4412..5017)
                     /gene="dhaL"
                     /locus_tag="ACHGNW_000779"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN6925118.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5045..6055)
                     /gene="dhaK"
                     /locus_tag="ACHGNW_000780"
     CDS             complement(5045..6055)
                     /gene="dhaK"
                     /locus_tag="ACHGNW_000780"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN6925119.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6030..6425)
                     /gene="dhaM"
                     /locus_tag="ACHGNW_000781"
     CDS             complement(6030..6425)
                     /gene="dhaM"
                     /locus_tag="ACHGNW_000781"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN6925120.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6579..6899
                     /locus_tag="ACHGNW_000782"
     CDS             6579..6899
                     /locus_tag="ACHGNW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN6925121.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7011..7868)
                     /locus_tag="ACHGNW_000783"
     CDS             complement(7011..7868)
                     /locus_tag="ACHGNW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6925122.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7976..9279)
                     /locus_tag="ACHGNW_000784"
                     /pseudo
     CDS             complement(7976..9279)
                     /locus_tag="ACHGNW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9276..9785)
                     /locus_tag="ACHGNW_000785"
     CDS             complement(9276..9785)
                     /locus_tag="ACHGNW_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925123.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9960..12095)
                     /locus_tag="ACHGNW_000786"
     CDS             complement(9960..12095)
                     /locus_tag="ACHGNW_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN6925124.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12178..13833)
                     /locus_tag="ACHGNW_000787"
     CDS             complement(12178..13833)
                     /locus_tag="ACHGNW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6925125.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13835..14149)
                     /locus_tag="ACHGNW_000788"
     CDS             complement(13835..14149)
                     /locus_tag="ACHGNW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6925126.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14319..14579
                     /locus_tag="ACHGNW_000789"
     CDS             14319..14579
                     /locus_tag="ACHGNW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN6925127.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14593..14907
                     /locus_tag="ACHGNW_000790"
     CDS             14593..14907
                     /locus_tag="ACHGNW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN6925128.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15637..16191)
                     /locus_tag="ACHGNW_000791"
     CDS             complement(15637..16191)
                     /locus_tag="ACHGNW_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN6925129.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16317..18245)
                     /locus_tag="ACHGNW_000792"
     CDS             complement(16317..18245)
                     /locus_tag="ACHGNW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN6925130.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18551..19267)
                     /locus_tag="ACHGNW_000793"
     CDS             complement(18551..19267)
                     /locus_tag="ACHGNW_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6925131.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19268..21091)
                     /gene="glpO"
                     /locus_tag="ACHGNW_000794"
     CDS             complement(19268..21091)
                     /gene="glpO"
                     /locus_tag="ACHGNW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN6925132.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21105..22601)
                     /gene="glpK"
                     /locus_tag="ACHGNW_000795"
     CDS             complement(21105..22601)
                     /gene="glpK"
                     /locus_tag="ACHGNW_000795"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN6925133.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22737..24197)
                     /locus_tag="ACHGNW_000796"
     CDS             complement(22737..24197)
                     /locus_tag="ACHGNW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925134.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24187..25482)
                     /locus_tag="ACHGNW_000797"
     CDS             complement(24187..25482)
                     /locus_tag="ACHGNW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6925135.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25814..26491)
                     /locus_tag="ACHGNW_000798"
     CDS             complement(25814..26491)
                     /locus_tag="ACHGNW_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN6925136.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26521..27612)
                     /locus_tag="ACHGNW_000799"
     CDS             complement(26521..27612)
                     /locus_tag="ACHGNW_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN6925137.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27713..28147)
                     /locus_tag="ACHGNW_000800"
     CDS             complement(27713..28147)
                     /locus_tag="ACHGNW_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN6925138.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28731..30188)
                     /locus_tag="ACHGNW_000801"
     CDS             complement(28731..30188)
                     /locus_tag="ACHGNW_000801"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6925139.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30185..32080)
                     /locus_tag="ACHGNW_000802"
     CDS             complement(30185..32080)
                     /locus_tag="ACHGNW_000802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6925140.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32138..33913)
                     /locus_tag="ACHGNW_000803"
     CDS             complement(32138..33913)
                     /locus_tag="ACHGNW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6925141.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33963..35459)
                     /locus_tag="ACHGNW_000804"
     CDS             complement(33963..35459)
                     /locus_tag="ACHGNW_000804"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6925142.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35653..37275)
                     /locus_tag="ACHGNW_000805"
     CDS             complement(35653..37275)
                     /locus_tag="ACHGNW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6925143.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37724..39037)
                     /locus_tag="ACHGNW_000806"
     CDS             complement(37724..39037)
                     /locus_tag="ACHGNW_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6925144.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT        11077 a         8387 c         6743 g        13023 t
ORIGIN      
        1 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
       61 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
      121 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
      181 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
      241 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
      301 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
      361 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
      421 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
      481 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
      541 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
      601 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
      661 actagcggat aaagtcgtga tcatacgcga aggaacagtc gtagaagaag gaacactcga
      721 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
      781 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
      841 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
      901 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
      961 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
     1021 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
     1081 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
     1141 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
     1201 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
     1261 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
     1321 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
     1381 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
     1441 agattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
     1501 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
     1561 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
     1621 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
     1681 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
     1741 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
     1801 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
     1861 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
     1921 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
     1981 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct
     2041 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
     2101 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
     2161 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
     2221 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
     2281 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
     2341 gcttcctcga atgggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
     2401 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
     2461 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
     2521 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
     2581 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
     2641 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
     2701 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
     2761 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
     2821 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
     2881 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
     2941 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
     3001 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
     3061 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
     3121 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
     3181 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
     3241 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
     3301 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
     3361 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
     3421 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
     3481 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
     3541 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
     3601 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
     3661 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
     3721 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
     3781 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
     3841 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
     3901 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
     3961 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
     4021 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
     4081 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
     4141 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
     4201 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
     4261 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
     4321 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
     4381 tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg
     4441 ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc
     4501 ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt
     4561 caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat
     4621 ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct
     4681 gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt
     4741 tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg actttcgata
     4801 tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa
     4861 aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa
     4921 tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg
     4981 tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga
     5041 ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg
     5101 tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga
     5161 cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga
     5221 acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt
     5281 ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag
     5341 aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga
     5401 tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa
     5461 gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag
     5521 ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg
     5581 caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga
     5641 tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca
     5701 atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta tccgcttcct
     5761 taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg
     5821 cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac
     5881 cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta
     5941 cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat
     6001 tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt
     6061 gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa
     6121 gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac
     6181 atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa
     6241 ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc
     6301 tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca
     6361 atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt
     6421 ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat
     6481 gaacaattca agtatataat aaatttctaa ttttgggaag atatgtgtag tgcttcttga
     6541 agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca acaacgaaca
     6601 gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag gtcattactg
     6661 ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa
     6721 aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa atggccgaac
     6781 gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat gaagtattag
     6841 gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc acgagataag
     6901 taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca ctcaagaatt
     6961 agatttttca gtctaacttc gaggtactct accttcccat cctctctttt ttatttcttt
     7021 ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa ggaataactg
     7081 acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt ggatggctct
     7141 gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa
     7201 tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt tgcttcaaaa
     7261 tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg
     7321 atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg atttaattgc
     7381 tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac agcttccgct
     7441 tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc cactttattt
     7501 ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt gttttttgca
     7561 gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa atgatcgatt
     7621 tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat cgtttctcct
     7681 gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca
     7741 gaagagctta tagttgttga aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct
     7801 gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg cttcattctt
     7861 tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta taagcaaaaa
     7921 tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc atcttttatt
     7981 tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc gttttgactg
     8041 ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa agcatatata
     8101 gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc agcgaagcta
     8161 gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg aatgtaaaag
     8221 taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat ccagcaaaaa
     8281 gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga tcaatgaaga
     8341 tgatcgttct tccaatatgt ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc
     8401 agacttctgt tccacctttt gcccaacgtt ttctttgtcg atatagcatc ttcaacgtca
     8461 caggtacttc catgaaaaag ataatctcag gagcaaaaac agataaccag tcatttaact
     8521 gatgatccca agcaatactg atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat
     8581 cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa agaccaccga
     8641 aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca accgtttgag
     8701 attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc gcagcaatat
     8761 gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct ggctccggta
     8821 ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa
     8881 acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta gcgtattttt
     8941 tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct gtcactaata
     9001 cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatct tcgatcacaa
     9061 tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc aagggaacat
     9121 ctacccattc tttttgctta tgtttgtata aaaacacata acaccagaca ccaataaacc
     9181 atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa tacataatag acaaacaagt
     9241 atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac cccattttct
     9301 ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac agaataaaaa
     9361 cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc ttcaagtaat
     9421 tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac ttttttgtat
     9481 agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc
     9541 aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa agcgtacaag
     9601 gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat aaaaggaaga
     9661 acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa ccagtaatta
     9721 ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct tgttactttt
     9781 ttcatatcct cacctcacgt ttttttgtta cgacgtagga gtttaccata catttcattt
     9841 cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca tttctcatca
     9901 gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt atctcttaat
     9961 tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc cgtgtacatt
    10021 tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat tacatgatca
    10081 aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa tccatatgaa
    10141 ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt gataactgcc
    10201 gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa ttcttcagag
    10261 gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac gatgtcgatc
    10321 ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct agaacggtaa
    10381 ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca acgcataata
    10441 tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac taaagtaaag
    10501 tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata atcttgtact
    10561 tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag taaatgacga
    10621 atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag
    10681 ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca tgccatttct
    10741 tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc ataagctaca
    10801 atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg cctatcttct
    10861 gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa aagatgattc
    10921 ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga
    10981 gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt ttttccattt
    11041 ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga ttgttttaga
    11101 ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg
    11161 atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg tgaaaccagc
    11221 tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac attcgaaata
    11281 tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt catcgtatct
    11341 cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt accacttcct
    11401 gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt gttttgttcc
    11461 gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc taagacttct
    11521 aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa acgatctctt
    11581 tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg acgaaccgta
    11641 taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga acgccaatca
    11701 taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata aaaggattct
    11761 tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact tttcaataaa
    11821 cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat ctgatcgatt
    11881 tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta actgtcaaaa
    11941 ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat ttttgccgta
    12001 tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac gttcttcaga
    12061 tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt tttttaaagt
    12121 ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta acgagtatca
    12181 tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa atgcaccatc
    12241 aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat agctacgtaa
    12301 tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta atcggtcccg
    12361 aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca attcttctgc
    12421 tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt catgccattg
    12481 tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca tgtttactgg
    12541 atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta aatcgaagat
    12601 cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca caccttttgc
    12661 cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca acttcagtaa
    12721 aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat gaatgacttc
    12781 aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg cggcagtcat
    12841 tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag aaattccgga
    12901 aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag aaataagtgc
    12961 atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat aaatatcagg
    13021 aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta attcgattcc
    13081 agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa ctaaatcagc
    13141 agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac
    13201 agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta taaaagctgc
    13261 catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca tgacttctaa
    13321 acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac caacgatcac
    13381 ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg gtacatttct
    13441 aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa ctggttttgc
    13501 accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct catgtacgac
    13561 ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac gcacatctaa
    13621 acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc gttcactgat
    13681 ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg gtcctttttc
    13741 aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag cagacattcc
    13801 acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact gttcgatcaa
    13861 accattgatt catgccacca cgtacattca ctacatcata accagatttc tttaattctt
    13921 tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc ttttctttgt
    13981 ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt atatgcccgc
    14041 cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa agcttttcct
    14101 ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt ttctgcacct
    14161 catttacatt tttataaata cctcatgggg tatattaaac aggagcttta ttaatatgtc
    14221 aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa taccgataag
    14281 gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat aataaaaata
    14341 tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa atgatcgaag
    14401 acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct agtattgatc
    14461 gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa cctgatagta
    14521 atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt aaaaagtaaa
    14581 ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg gtcagaaaca
    14641 gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt cgtatctgta
    14701 aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt agatttgcaa
    14761 ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc agaaagttct
    14821 ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga actgcaatcc
    14881 caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt gatcagcttc
    14941 aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg
    15001 tgaaaacgct gtcctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
    15061 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
    15121 gttgtgaaat cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca
    15181 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag
    15241 agattgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc
    15301 tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg
    15361 aagaggttgt aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct
    15421 gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac
    15481 caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact
    15541 tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga gtctatttat
    15601 caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg
    15661 gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat
    15721 gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata
    15781 gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga
    15841 tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa
    15901 cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt
    15961 atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat
    16021 gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa
    16081 tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa
    16141 aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc
    16201 ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat
    16261 ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg agataatcac
    16321 ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt
    16381 actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa
    16441 tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc
    16501 agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta
    16561 tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac aaatatccga
    16621 tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt aactgctgtc
    16681 ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct
    16741 tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct
    16801 cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc gaaagccata
    16861 gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc ataatgggtc
    16921 gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat cgctagtgca
    16981 gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa cgtattcctt
    17041 tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt actcaacgca
    17101 ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac agcaagctct
    17161 gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa taaaccgact
    17221 gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa gcctcgatag
    17281 tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg atgtctgcct
    17341 tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt gatttctcgg
    17401 ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat caataaatcg
    17461 atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg atattcttca
    17521 ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat cacagttggt
    17581 tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc tattttattt
    17641 ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat cattgcttct
    17701 tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga ctggatttct
    17761 ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg
    17821 actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa atcaggaaga
    17881 tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa aaactttgtg
    17941 atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg ctgcagaatc
    18001 aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca
    18061 tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg atccaagaca
    18121 gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata gtttccaatt
    18181 gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat catttcgttg
    18241 tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag ggcctagaga
    18301 taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt agaatacaaa
    18361 gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt tgtttattgg
    18421 ctattcgact tactgattag ataccagcta cactcacaaa acatacccca ttcttctagg
    18481 tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa ctttagtatg
    18541 ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct ccgatcattg
    18601 gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta gctattggca
    18661 ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca tatcctgttg
    18721 gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat acgttcgttc
    18781 caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca aatgtaccaa
    18841 ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt gcaaacgttc
    18901 ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa agccaaacaa
    18961 gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg acaagattcc
    19021 aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa tgagctgggc
    19081 ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca aatgcgatga
    19141 caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca cacacaccat
    19201 ctccaagcaa aactaggatc agtgtcccaa taaactctcc catgatttga gtcatatcac
    19261 tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc aatgcttcta
    19321 aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca gcaattgcat
    19381 cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga tgatttgtac
    19441 gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca taacgtaacc
    19501 gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct aattcaaaga
    19561 tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct tcctctaaac
    19621 cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta gttggatcga
    19681 agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct attccgtatt
    19741 cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta cggtaatcgg
    19801 tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa gaactacctc
    19861 tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc aacacgtctt
    19921 ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg acaactttat
    19981 tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa ccagaattac
    20041 caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat gtgatatttg
    20101 cttctggata gcgatgattg atcacatcca atagataatc tacatcttct tgtgtgactt
    20161 tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt
    20221 ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag tatgtgggtt
    20281 gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt ggacgcattt
    20341 taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa ggaccactcg
    20401 tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg tcacgagctt
    20461 tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt ttacttacta
    20521 gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg gcatcattgt
    20581 tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa aacggttctc
    20641 tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct gtcacatttg
    20701 ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag gttgttgctc
    20761 cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt ggggcaatac
    20821 tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca aatgtcttca
    20881 gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat
    20941 cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact gccacaccgg
    21001 caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc attttttgaa
    21061 tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc aaatgcttgg
    21121 gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc ttcctccatt
    21181 tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt atctttccaa
    21241 taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc caagttttct
    21301 gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata ttcattgttt
    21361 gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc ttgcatcgta
    21421 tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat gatatcttct
    21481 tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc ccaataagga
    21541 gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt agagcgtttc
    21601 gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct cagccattga
    21661 atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt tccatttatg
    21721 ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc accggtattc
    21781 atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc gaatgccatt
    21841 tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc ttcgcttcca
    21901 taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac ttttggtaac
    21961 atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag ctgatggata
    22021 ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg tccatcggtc
    22081 agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt ttcagctcgt
    22141 tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga gaaataggca
    22201 tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt ttttaagtta
    22261 tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat tggacgtcct
    22321 gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat cccagcaatc
    22381 tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg tacagaattc
    22441 cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg cgtgaactct
    22501 ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg tgagctagtt
    22561 gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc ctccattata
    22621 tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag aaactgtcaa
    22681 aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt ttttaatcaa
    22741 ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac
    22801 gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc
    22861 aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt
    22921 tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt
    22981 ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg
    23041 atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct
    23101 gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt
    23161 tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg
    23221 ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga
    23281 cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt
    23341 ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca
    23401 aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga
    23461 tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc
    23521 ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg
    23581 agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc
    23641 aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct
    23701 tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat
    23761 aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc
    23821 tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact
    23881 tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac
    23941 aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt
    24001 cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat
    24061 tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt
    24121 cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat
    24181 aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg
    24241 gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttc
    24301 tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc
    24361 tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat
    24421 tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc
    24481 ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg
    24541 ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt
    24601 acggacagcg ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac
    24661 ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac
    24721 ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg
    24781 agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat
    24841 agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg
    24901 atttttcatt tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat
    24961 tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat
    25021 ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg
    25081 ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga
    25141 aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt
    25201 ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta
    25261 aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc
    25321 actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga
    25381 ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc
    25441 atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta
    25501 taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa
    25561 ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc
    25621 tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt
    25681 caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag
    25741 gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac
    25801 acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag
    25861 attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg
    25921 ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt
    25981 cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag
    26041 ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa
    26101 cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg
    26161 cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat
    26221 ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat
    26281 cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta
    26341 catatccgcc tttaggactg acaaaatctg catcgatccc attttttact aatacatcat
    26401 aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt
    26461 ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg
    26521 ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata
    26581 tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc
    26641 ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac
    26701 ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg
    26761 aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat
    26821 tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc
    26881 aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa
    26941 acgataccct acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt
    27001 taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg
    27061 tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc
    27121 gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt
    27181 ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc
    27241 aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc
    27301 accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc
    27361 acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc
    27421 gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt
    27481 ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat
    27541 aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc
    27601 aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct
    27661 atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt
    27721 tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc
    27781 ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa
    27841 attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga
    27901 cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga
    27961 gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa
    28021 aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca
    28081 tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct
    28141 tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa
    28201 aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt
    28261 caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg
    28321 tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt
    28381 tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg
    28441 ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    28501 attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag
    28561 gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    28621 tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt
    28681 aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt
    28741 tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc
    28801 tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt
    28861 tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct
    28921 actccatctt tgatagcttc cttcatctgc tcaatatgct gtttcaaata gtcaatgcgg
    28981 taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca
    29041 gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc
    29101 ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca
    29161 gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac tgcactagac
    29221 atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct
    29281 acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa
    29341 tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt
    29401 tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg
    29461 acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac
    29521 tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact
    29581 cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat
    29641 tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata
    29701 agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg
    29761 gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa
    29821 aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat
    29881 gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta
    29941 aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc
    30001 tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta
    30061 ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc
    30121 catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat
    30181 tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac
    30241 tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat
    30301 atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt
    30361 tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac
    30421 cgatatggat cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg
    30481 gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga
    30541 tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat
    30601 caattatttc acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga
    30661 tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat
    30721 aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc
    30781 ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc
    30841 cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag
    30901 gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt tttactttct
    30961 tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca
    31021 tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta
    31081 caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac
    31141 cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg
    31201 cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa
    31261 tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa
    31321 tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta taccttgcta
    31381 gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg
    31441 taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt
    31501 ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat
    31561 tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga
    31621 gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg
    31681 cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag
    31741 atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat
    31801 ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt
    31861 gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg
    31921 cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg
    31981 ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc
    32041 ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttataa
    32101 tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg
    32161 cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct
    32221 tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt
    32281 ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta
    32341 ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt
    32401 gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg
    32461 tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct agaacactag
    32521 gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat
    32581 catattccgt tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt
    32641 ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa
    32701 ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg
    32761 caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg
    32821 ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa
    32881 aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta
    32941 gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg
    33001 tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca
    33061 aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat
    33121 agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat
    33181 acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag
    33241 aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta
    33301 atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc
    33361 ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt
    33421 cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc
    33481 gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga
    33541 aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa
    33601 tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga
    33661 tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact
    33721 ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt
    33781 tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc
    33841 ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct
    33901 ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa
    33961 aatcatttga agaaagatct atattcaagg tttttaccat tagactggat aatttgttta
    34021 taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta
    34081 tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg
    34141 attggtcccc accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt
    34201 tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca
    34261 tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg
    34321 tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt
    34381 tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg
    34441 cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct
    34501 gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc acagtcttct
    34561 tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa
    34621 aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat
    34681 gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt
    34741 ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa
    34801 atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc
    34861 aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa
    34921 tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg
    34981 attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa
    35041 tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca
    35101 taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc
    35161 catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct
    35221 tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa
    35281 gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc
    35341 ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat
    35401 tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata
    35461 actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca
    35521 agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt
    35581 aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc
    35641 taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt
    35701 gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg
    35761 aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc
    35821 atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc
    35881 ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac
    35941 agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg
    36001 aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt
    36061 cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc
    36121 agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc
    36181 atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt
    36241 catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt
    36301 tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc
    36361 ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa
    36421 gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg
    36481 gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt
    36541 tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat
    36601 ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt
    36661 gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga tccagaaatt
    36721 catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg
    36781 cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc
    36841 actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata
    36901 atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc
    36961 agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc
    37021 aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact
    37081 gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc
    37141 aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc
    37201 atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca
    37261 ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat
    37321 ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta
    37381 tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag
    37441 gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc
    37501 agctgtccct tggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt
    37561 tttctctaat actcaggcca gaacactgat ttcacaacct ctttataaac ggtgttcact
    37621 cagctgtccc tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt
    37681 ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata
    37741 tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc
    37801 ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg
    37861 aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta
    37921 agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg
    37981 ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca
    38041 agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag
    38101 ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag
    38161 ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac
    38221 ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga
    38281 atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa
    38341 ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca
    38401 aacctgtgac cagacctaaa atcgttgtag ctgctatccc gatgaagatg ccacctggta
    38461 cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac
    38521 caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt
    38581 cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat
    38641 ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa
    38701 tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac
    38761 aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac
    38821 gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca
    38881 ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat aagatataag
    38941 ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt
    39001 tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac
    39061 attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat
    39121 actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca
    39181 tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta
//
LOCUS       DAZBTH010000016        37538 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000016 DAZBTH010000000
VERSION     DAZBTH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.016"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..358)
                     /locus_tag="ACHGNW_000807"
     CDS             complement(194..358)
                     /locus_tag="ACHGNW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925145.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            653..1981
                     /locus_tag="ACHGNW_000808"
     CDS             653..1981
                     /locus_tag="ACHGNW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN6925146.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2183..3016
                     /locus_tag="ACHGNW_000809"
     CDS             2183..3016
                     /locus_tag="ACHGNW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN6925147.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3032..3769
                     /locus_tag="ACHGNW_000810"
     CDS             3032..3769
                     /locus_tag="ACHGNW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN6925148.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3924..4892)
                     /locus_tag="ACHGNW_000811"
     CDS             complement(3924..4892)
                     /locus_tag="ACHGNW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN6925149.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5124..5957
                     /locus_tag="ACHGNW_000812"
     CDS             5124..5957
                     /locus_tag="ACHGNW_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN6925150.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5991..6830
                     /locus_tag="ACHGNW_000813"
     CDS             5991..6830
                     /locus_tag="ACHGNW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6925151.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6856..7416
                     /locus_tag="ACHGNW_000814"
     CDS             6856..7416
                     /locus_tag="ACHGNW_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6925152.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7615..9336
                     /locus_tag="ACHGNW_000815"
     CDS             7615..9336
                     /locus_tag="ACHGNW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN6925153.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9501..10643
                     /locus_tag="ACHGNW_000816"
     CDS             9501..10643
                     /locus_tag="ACHGNW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6925154.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10659..11870
                     /gene="thiI"
                     /locus_tag="ACHGNW_000817"
     CDS             10659..11870
                     /gene="thiI"
                     /locus_tag="ACHGNW_000817"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN6925155.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12443..13090
                     /locus_tag="ACHGNW_000818"
     CDS             12443..13090
                     /locus_tag="ACHGNW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6925156.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13219..13435
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13483..16128
                     /locus_tag="ACHGNW_000819"
     CDS             13483..16128
                     /locus_tag="ACHGNW_000819"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN6925157.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16438..17751
                     /locus_tag="ACHGNW_000820"
     CDS             16438..17751
                     /locus_tag="ACHGNW_000820"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN6925158.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17741..18394
                     /locus_tag="ACHGNW_000821"
     CDS             17741..18394
                     /locus_tag="ACHGNW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6925159.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18449..19000
                     /gene="radC"
                     /locus_tag="ACHGNW_000822"
                     /pseudo
     CDS             18449..19000
                     /gene="radC"
                     /locus_tag="ACHGNW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19131..20216
                     /locus_tag="ACHGNW_000823"
     CDS             19131..20216
                     /locus_tag="ACHGNW_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925160.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20213..22105
                     /locus_tag="ACHGNW_000824"
     CDS             20213..22105
                     /locus_tag="ACHGNW_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN6925161.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22112..22489
                     /locus_tag="ACHGNW_000825"
     CDS             22112..22489
                     /locus_tag="ACHGNW_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFN6925162.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22643..23425
                     /locus_tag="ACHGNW_000826"
     CDS             22643..23425
                     /locus_tag="ACHGNW_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFN6925163.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23551..24282)
                     /gene="erm(A)"
                     /locus_tag="ACHGNW_000827"
     CDS             complement(23551..24282)
                     /gene="erm(A)"
                     /locus_tag="ACHGNW_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFN6925164.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24340..24399)
                     /locus_tag="ACHGNW_000828"
     CDS             complement(24340..24399)
                     /locus_tag="ACHGNW_000828"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFN6925165.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            24791..25453
                     /locus_tag="ACHGNW_000829"
     CDS             24791..25453
                     /locus_tag="ACHGNW_000829"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6925166.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25688..25831
                     /locus_tag="ACHGNW_000830"
                     /pseudo
     CDS             25688..25831
                     /locus_tag="ACHGNW_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            25981..26181
                     /locus_tag="ACHGNW_000831"
     CDS             25981..26181
                     /locus_tag="ACHGNW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6925167.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26435..28114)
                     /locus_tag="ACHGNW_000832"
     CDS             complement(26435..28114)
                     /locus_tag="ACHGNW_000832"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6925168.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28116..28328)
                     /locus_tag="ACHGNW_000833"
     CDS             complement(28116..28328)
                     /locus_tag="ACHGNW_000833"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN6925169.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            28721..30451
                     /gene="efrA"
                     /locus_tag="ACHGNW_000834"
     CDS             28721..30451
                     /gene="efrA"
                     /locus_tag="ACHGNW_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN6925170.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30448..32208
                     /gene="efrB"
                     /locus_tag="ACHGNW_000835"
     CDS             30448..32208
                     /gene="efrB"
                     /locus_tag="ACHGNW_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN6925171.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32303..32500
                     /locus_tag="ACHGNW_000836"
     CDS             32303..32500
                     /locus_tag="ACHGNW_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925172.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            32653..33138
                     /locus_tag="ACHGNW_000837"
     CDS             32653..33138
                     /locus_tag="ACHGNW_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN6925173.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33242..33835)
                     /gene="clpP"
                     /locus_tag="ACHGNW_000838"
     CDS             complement(33242..33835)
                     /gene="clpP"
                     /locus_tag="ACHGNW_000838"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN6925174.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            34015..34542
                     /locus_tag="ACHGNW_000839"
     CDS             34015..34542
                     /locus_tag="ACHGNW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925175.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            34633..35370
                     /locus_tag="ACHGNW_000840"
     CDS             34633..35370
                     /locus_tag="ACHGNW_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6925176.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35374..36291
                     /gene="miaA"
                     /locus_tag="ACHGNW_000841"
     CDS             35374..36291
                     /gene="miaA"
                     /locus_tag="ACHGNW_000841"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN6925177.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36293..37522
                     /gene="hflX"
                     /locus_tag="ACHGNW_000842"
     CDS             36293..37522
                     /gene="hflX"
                     /locus_tag="ACHGNW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN6925178.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12590 a         6180 c         7860 g        10908 t
ORIGIN      
        1 gtagtagcgc atgactgtta atcatgatgt cgtaggttcg agtcctactg ccggagtaaa
       61 taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct tttattatta
      121 tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa agagtagagt
      181 tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa atcagcattc
      241 ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca aagttatctg
      301 tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg tttttcattt
      361 tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt ttgaaagcta
      421 tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt ttcgttatct
      481 catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt tttcttcatg
      541 aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat tgtatttcta
      601 aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt ggatgaaagc
      661 tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata tcaaacagca
      721 ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa atgtttcagc
      781 acatgtggag aatacaccgg tattttctat agatttggct acggggaaag tggctaatca
      841 aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc ataagatgct
      901 gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt tctgggataa
      961 atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag aggcaatcga
     1021 tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc aatgggatat
     1081 gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc cggaaagtgc
     1141 gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc gtaaagatgc
     1201 agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg aagaaaagaa
     1261 agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa cacctccaaa
     1321 agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc gaggactgac
     1381 accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg ttagcgtgat
     1441 caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga tgctaggaaa
     1501 tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt tcaaaaaatt
     1561 agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg taggtcaatc
     1621 ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg atctatttga
     1681 tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc tgatgaccca
     1741 tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat ggaaagtcga
     1801 aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg atgcctggat
     1861 ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag agcaattaaa
     1921 tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg ctttagcata
     1981 gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt aagccagctc
     2041 tttttatctt taaagtccat tccctgtttt ttatggtccc tgtgctataa ttctaaataa
     2101 aggataagga ttggaaataa tccttttatc attaaaaata tgatgacgaa gtagaataaa
     2161 attgggagta ggggggaaag tcatggatat ccaaactgtt ttatctgatc tgaaattaaa
     2221 tggtaaagtg attccagttg ttggtggcga tgttaatcag acctatcgta taaaaaccga
     2281 acatcgtgct tattttttaa aaatccatcc gaatgtaaaa aaaggatttt ttgaagcaga
     2341 agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta ccggatacgt atatgctagg
     2401 tgaaacaagt gaaggcgctt atttgttaat ggaatggatc gagccgggaa aaggggatca
     2461 acgagatctt gctgcagctt tagctaatct ccatcagcaa acagcgccac aatttggttt
     2521 tcgtaaagat aactatttgg gtacacttgt tcagaaaaat tcttttgaag aagattggtg
     2581 gacttttttc tttaaagatc ggcttgaatc ccagatttct ttggcagaag aaacgaatcg
     2641 atggaatgta cagcgtcaag aaaagtatct gcgatttaaa gaacgtgtat tgaaatcggt
     2701 tgaaccaaaa aagatcactc cacgcctctt gcacggagac ctgtggagtg gtaatgtgtt
     2761 ctttgaccag caagggcatc cagtttttgt cgacccagct gtttcttatg ggaatcggga
     2821 acaagatatt gcaatgagcc agttattcgg tggtttccga ccggaatttt tagatgctta
     2881 tcaaactata tttccattag aaaagggatg gaaagaccga ctgccgattt accagttata
     2941 ttatcttttg gcacatttga atatgtttgg agaaagttac ggttcccaag tagatcaatt
     3001 actagaaaat ttttaagagg agggcgtgtt tttggcaaaa aaatatacca aaaagcatga
     3061 agtttcatat tatgagtgtg acatcaacca aacgatgaca tttccttcga tgttaggagt
     3121 agttatcaaa acgtctgaag atcaaagcga ccagttggga agaggcagtg aattcgtcaa
     3181 tagctttgga cttacttggg tcatcacgaa ttattccatg gagattaatc gtctccctag
     3241 agtcggtgaa gtaatctcag taacgactca agccatggag tacaataagt ttttctgtta
     3301 tcggaacttc tggatccatg atgaagatgg gaaagaatta gtcaaaatag aatctgtttt
     3361 cgtattgatg gattttgtga atcgaaaaat gagcagtgtt aatgaagaga tcattgctcc
     3421 ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa aaaattgaaa agattgattc
     3481 gggcttcatg ttgccatatc gtgttcgttt ttatgatatc gatagtaatc agcatgttaa
     3541 taatgcgatg tattttaatt ggatcattga cgtattagga tatgactttt tgactacgca
     3601 tgttccagaa aaagtaatta ttcgatttga taaagaagta gagtacggga atgagatcga
     3661 aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa acaaaacatg aaatccgaat
     3721 gcaagaacag ctgtattgtg aagcaaatat tatatggaaa aataaataaa aaagaggttg
     3781 tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt ttctcaaatt
     3841 tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct ctctttttaa
     3901 gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc ttcacccgac
     3961 aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt aatactgtta
     4021 cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc ataaacaggc
     4081 tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg gacaagaagc
     4141 ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc ttcgattacc
     4201 aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat cccagcatat
     4261 gctttaacga tcgggatagt cccatctaca gcggaaatgt ccagtcgctt tgtttctaaa
     4321 agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc caatgtaggt
     4381 gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc agaatggatc
     4441 tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact tgcgacacga
     4501 atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga acgcattgcc
     4561 ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa agcagtttct
     4621 tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc ataagcggac
     4681 aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga gggaagattg
     4741 aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc atctaataat
     4801 gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat ggcgatcgtt
     4861 ccgccggtgt gtagtacaag tatttttttc attttacaga cttccttacg aataattttg
     4921 catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag tgaagaataa
     4981 cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag actcaacctt
     5041 gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc agccaaggaa
     5101 atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt aaagaacatg
     5161 acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt ctacccttag
     5221 tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag aattggataa
     5281 gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag
     5341 gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt ttagatgtta
     5401 tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc ccctcatcat
     5461 tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg tttgagggtt
     5521 atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt cttgaacgta
     5581 caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag ccacagttgc
     5641 tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct ctttttcgat
     5701 tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt aaaaaaagaa
     5761 cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc aaagtagttg
     5821 attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt gatgcggctg
     5881 aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc aatccaaccc
     5941 aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct atgatcaaag
     6001 ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa
     6061 caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta gcaacggggc
     6121 gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg tatgtcgtgt
     6181 ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct tttgaacaaa
     6241 aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc gtttttggtg
     6301 cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt tttatcaagc
     6361 gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg
     6421 tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta aaagaaccaa
     6481 tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa catcgctttc
     6541 ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca aaaggtggtt
     6601 caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg gaagaaatta
     6661 tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga attggagtgg
     6721 ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact caatcaaatg
     6781 aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag tcaatgaaag
     6841 aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac attccacaaa
     6901 acttttgatc caagaagacc agagataccc acggctttta cagaaaatga agctcataca
     6961 cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc acctaataaa
     7021 gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc agaagaaaaa
     7081 atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga tgcactgaca
     7141 gatatccttt actttactta tggttctttt tcattactag gaatagtgcc tgatcgaata
     7201 ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa acctcattat
     7261 gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata tgctccagaa
     7321 cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa gaagaaatcc
     7381 gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta tttttgttat
     7441 ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat gaaaatagta
     7501 tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga tttatgccaa
     7561 gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc ataaatggga
     7621 aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt cgtctttttg
     7681 gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag
     7741 gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg
     7801 gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga catatctact
     7861 tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt
     7921 cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga ccaaatggag
     7981 aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga agaacgcaac
     8041 tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa
     8101 gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa agtgatcgaa
     8161 aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga agcccgcgag
     8221 gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa aaaaattcca
     8281 ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat cagtgatact
     8341 tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc tgaagatatc
     8401 gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa
     8461 acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata tgatatctta
     8521 gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat tgcagagtat
     8581 atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca cacatctcaa
     8641 agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa
     8701 gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga aattccttat
     8761 tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa
     8821 caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa
     8881 aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga aaaacaacgt
     8941 ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga ccgtattgaa
     9001 gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt
     9061 gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt agtagatgca
     9121 gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac acacgaagaa
     9181 gtgagagaag caattgataa aagctactat ttattcaata aagaatatca ctatcaagaa
     9241 gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt caaacgcatc
     9301 gaagattttt attttaatca tccagactta gtataaagca aaaaagttag taaaccagca
     9361 agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa aattgaaaca
     9421 agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata gaaaagaatt
     9481 tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc ccaatctttc
     9541 cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat ccatcaagct
     9601 tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag cagatcgctg
     9661 atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca gaaggagata
     9721 actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat atgatcattt
     9781 caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg
     9841 aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat
     9901 taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa gtaggatcga
     9961 ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt catttccatg
    10021 tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca gaaagagttg
    10081 attttgcaac attttcagct cataaattcc atggcccacg gggaactggt ttcatttatt
    10141 ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa agaaatcagc
    10201 gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta cgtttatcat
    10261 tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct ctaatcaatg
    10321 cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat gcaccgcata
    10381 ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa
    10441 aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg gcaagcagta
    10501 cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga atcagcttag
    10561 atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag cagctttata
    10621 agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt gaaattatgg
    10681 tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc atgcaactag
    10741 cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat gctgatcggg
    10801 accggatgca tcttttattg aatggcgcag acgggcagct agttattcca aaactagcaa
    10861 aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt
    10921 tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa acatttaaaa
    10981 ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg aatcaaacat
    11041 taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt caaccagata
    11101 ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg
    11161 gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt tctgggggga
    11221 ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt gaagctgttc
    11281 attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa gatctaacag
    11341 ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt accgagattc
    11401 aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact cgtcgaatga
    11461 tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg
    11521 aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa
    11581 cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc attgaagtag
    11641 cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc tgtacgattt
    11701 ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa tatgaagagc
    11761 gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg gaagagatac
    11821 aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa gaggttatga
    11881 aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc catgtatttt
    11941 accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca agaagttatg
    12001 gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga tcaagctaca
    12061 gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt gacagaagtg
    12121 ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt ttgtgaaatt
    12181 tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt tgcgaaaaag
    12241 ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata ttttacgttt
    12301 ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt ctcacaccct
    12361 tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta aatatgtgaa
    12421 attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa aagcaactgc
    12481 gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag gtataagtag
    12541 aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga tccgcagaga
    12601 cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc cttttcttat
    12661 cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat tagtaggata
    12721 tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa atctaaatat
    12781 cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg gagaaatgat
    12841 ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag tagcaatctc
    12901 tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag gaaatgtaac
    12961 cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca atgttcaata
    13021 cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt actcagaagt
    13081 tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc gtaattcttt
    13141 caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg actaaaaaaa
    13201 aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca cattcattct
    13261 tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt agcttgggag
    13321 tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac ttgagaggca
    13381 ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc tagaatacga
    13441 aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga agaaaataac
    13501 ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca ttggttagaa
    13561 caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat cgtaatcccg
    13621 cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac cttgcaagat
    13681 atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc tggtatggac
    13741 catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca agggatttct
    13801 cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa agaagagtat
    13861 gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta cagtagggaa
    13921 cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt taccttatac
    13981 gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca agcacgaaca
    14041 gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta tcatatgagc
    14101 tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc agaaacgatg
    14161 ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc gctcatcggc
    14221 aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga tgaatatgta
    14281 gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc aaacgacttt
    14341 gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga tggtacgatg
    14401 aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg
    14461 caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca cagtgtgggt
    14521 cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa
    14581 atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc agtggaattc
    14641 ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca tgattgggta
    14701 atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa agaaacaggt
    14761 gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca agatcctgat
    14821 gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg ctggccagat
    14881 acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac cggttatgac
    14941 atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac tggcgaacgt
    15001 ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg gaagatgagt
    15061 aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg agcagatgct
    15121 ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt cagctatgaa
    15181 aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg tttcgttatc
    15241 atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa atcagtggct
    15301 gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga gctatttgat
    15361 cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga tgatttttgt
    15421 gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt ttcaaaacaa
    15481 accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt gcatccaatc
    15541 atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa ttctctagta
    15601 gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc taaagggatg
    15661 gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt gaatacacca
    15721 ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga tcaattttta
    15781 gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac gatatcaagt
    15841 gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga aatcttcttg
    15901 ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa agaactggaa
    15961 aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct
    16021 aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta tttagagaag
    16081 caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag
    16141 agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg ttacaggaaa
    16201 gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa ttttgtcgca
    16261 tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc atgtatcaag
    16321 agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct ttgtcatact
    16381 tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga attgttgatg
    16441 acgattgaag aagcaataga atggatccat agtcgattac catttggttc acgtcctggc
    16501 cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa agtaaaaacg
    16561 atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag aagcctatta
    16621 gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac gttcaatgaa
    16681 cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct tgtaaaaaaa
    16741 tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat tactgaattc
    16801 gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga cgtagcgatt
    16861 atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc aatgctgacg
    16921 ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat tgaagaaatc
    16981 gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg aaatattgcc
    17041 ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta
    17101 tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg agaagtattt
    17161 gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt agggcaacat
    17221 caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca attggaaaag
    17281 ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg gccaggaaga
    17341 atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa tacgcatgca
    17401 atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat caacattttg
    17461 ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca gcaagtacca
    17521 aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt aagtgaagta
    17581 tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg attagcagat
    17641 atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact ttatttcatt
    17701 tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata atgcagtgat
    17761 ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag ccacacaagt
    17821 agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat tcttaggtgt
    17881 ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg gcaaaacaag
    17941 tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt cgcaaggagc
    18001 ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac atcagattcc
    18061 caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga agaagaacaa
    18121 tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag caaaaccaga
    18181 cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag aaacattgat
    18241 tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc cggtagtcat
    18301 ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg gggttgtttc
    18361 ttctttacat gaaatccctg cattgttaaa ataaaaatcc atctcttttc gtatcttatt
    18421 ttagataaga tagaaaggag atgttttttt gggaaaacga ttgatcaaag aagtacctac
    18481 tagctcttta ccaagggaac gtatggaaat ttatggcgcg gaagctttat cggatcagga
    18541 gcttttggca atcttactgc gtaccggtca acacccttat agtgtgatgt cgattgctgg
    18601 aaatctgtta aaaacgtttg gaggactggc ctctttacga caggctactt tgcatgagtt
    18661 agaagagatt cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt
    18721 aggtcgacga attcagacgg atcatgaaag agcggctcca ccggttcgta ccagctacga
    18781 attggcccag caattgattc tggaaatgaa agatcacaaa caagaacatt tggtttgtat
    18841 ttatttggac acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa
    18901 tcagtcagtc gctcatccta gagaaatttt ccattatgct gtacgttatt gtgcagcaag
    18961 gatcgtttta gcgcataatc atccttctgg taatgtttta accatagtat aataaataat
    19021 agtagttaaa gtagtttaag aggagatatt aaagggatat tttactttaa ataaaaatta
    19081 acaaggttta taatttgtag tagttaaagt atttaaagag gtgggaacat gtgaaggttc
    19141 aaaggataga agtggagaat aagccgtatc cattgtattt attactagat aaagaatacc
    19201 agctaataga accagtaatg aaatttatta aatacttaga taatactggt aagtctccta
    19261 ataccattaa agcatactgc tatcatttaa agttgctgta cgagttcatg gaacagagag
    19321 gtgttattct taatgatatt aactttgagt tgttagcaga cttcgtaggt tggttgagat
    19381 atccttcagc atcaaatgta attgatcttc agtcaaaaaa agccataaga gaagaaacga
    19441 cagtgaatac aattttaaat gtagttatga gttttcttga ttatttaagt agattaggag
    19501 aatttaaatc aattgatgta tttaaacaag caaagggaag aaatttcaaa ggatttttac
    19561 atcatgttaa taagggtaga taccaaaaga atgtcttaaa gttaagggtt aaaaagaaac
    19621 agataagaac attgagatca gaggaagtta agcaaattat tgatgcttgt catacgaaaa
    19681 gagataaatt aattttaatg cttatgtatg agggtggttt aagaatcggt gaagtgttat
    19741 cgcttaggct tgaagatatt gtcacttggg acaatcaaat ccatttaaca cctagagatg
    19801 ttaatgttaa tgaagcttat attaaattaa ggaaggaaag aacaatacat gtgagtaaag
    19861 aacttatgtc actttataca gattacttga tatatgagta tagtgaggaa ttggagcatg
    19921 attatgtttt tatttcctta aaagaaggct attttgggaa accactaaag taccaaagtg
    19981 ttcttgatct agttagaaga atagttaaaa ggactggaat agaatttaca tcacatatgc
    20041 ttcgccacac tcacgcaacg cagctaatta gggaaggatg ggatgttgcg ttcgttcaaa
    20101 agagattagg tcacgcacat gttcagacaa cgttaaatac ctatgttcat ctttcagatc
    20161 aggatatgaa aaatgagttt aataaatacc tcgagagaaa ggagcataag aaatgaatgc
    20221 ttctagtaaa aggaaaatta ttagtcagag tgagattagc aaaaaaatag ctgtaatgaa
    20281 tgaagaaatg caggggtttt gggctaataa tagttgggat ataagaaaat gtccacatcc
    20341 ttctgccata gaattaagta agaatcctgc tttaaggaat cgttgggttc gttttgaacg
    20401 tgttaaaaat ctgtggttaa gaacagaatt gaaatatttt tatttttacc atttaaacaa
    20461 tggaatatgg aatgcaaaaa ctgtctggat tagaaaagga acagtaatta ataaaatgtt
    20521 agatttctta gatttaaagt atcctagcat tacttcaatt actgaagttc ctattgaaaa
    20581 agcaatgacg gagtatagaa cttatttgac aaaacggggt gttagaatta ccactactaa
    20641 ttataagatt actgctaatc aagaaaaaac acctgtaaaa gctaattcct actatgttac
    20701 taatctaaaa caatttatgg agttttatga gaacttttat tttgatggag aggagtggga
    20761 taaagacgtt tgggatagac gtaacttacc tttgccagat gataaggtta acccaacaca
    20821 atatgaatat acaattaact ttaaagggtt tcggaataca tattttaaac aacttgtaaa
    20881 aagatattgt aagttgagat tgaacatgga tagcttttcc tatgtaagtg atattgccca
    20941 aagacttaaa gagttcttta attttctgga catgaaattt aaacaagttc agagagtaca
    21001 ccaattaacg agagtggaaa ttgaagcata tttaagtgaa ctaaacatga tgggaataaa
    21061 acctagtaca ataactggga ggatctctat attggaagga ctatttagta cccttcttag
    21121 gctagaatgg gatgatgttc cttccaaaat attaatttat tctgaggact atccgaaaat
    21181 accaagagca aaaccacgct ttatagatga attcgtccta gagcaattga acagtcatct
    21241 tgataaatta cccgaatata tagctacgat gactatgatt gttcaagaat gtggaatgag
    21301 gataagtgaa ttgtgcacct tgaaaaaagg ctgtctatta gaggacaaag atggagattt
    21361 ctttttaaag tattatcaat ggaaaatgaa aaaggagcat atagttccaa tatctaaaga
    21421 ggtagcttta cttattaaag ttcgggaaga taaagtttca gaggaatttc cagatagtga
    21481 atacctcttt ccaagaaaag atggatcgcc attaaaacaa gaaacattta gaggtgagtt
    21541 aaataaatta gcttatgagc aaaatatagt ggataaatca ggtgagattt atagattcca
    21601 tgcccatgcc tttcgccata cagtaggaac aagaatgatt aacaacggga tgccccagca
    21661 tattgtgcag aaatttttgg ggcatgaaag cccagaaatg acaagcagat acgctcatat
    21721 ctttgatgaa actctaaaaa atgaatttac taaatttcag gaaaaactgg ttaccaataa
    21781 tggagatgtg cttgatctag atgaagataa tgaagtcgat gatgtagagc ttcaatggtt
    21841 caagaaaaat ataaatgcac aagtgcttcc aaatggttat tgtagattgc cagtagtagc
    21901 aggtggttgt ccacatgcga atgcatgctt agattgcact cacttctgta ccagtaagca
    21961 attcttacca cagcacgaag aacagttaga gcgtacagaa gagttattag ccatagctaa
    22021 ggataaacaa tggcaaagac aagtagagac taatagccgt gttaaagagc gtttagaaca
    22081 aatcattgga agtttgacgg ggtaattatc aatggataaa caagttagaa atacaacaga
    22141 aattgtacgt ttggcgaagc agaaatcaaa aaagacaagg gaaaaagtag acaaagcgat
    22201 ttctaaattt tcgattgaag gtaaagttat taattttaat tcaatagcaa aggaagctaa
    22261 tgtttctaaa tcatggcttt ataaggaaca cgatattagg caaagaatcg aatcccttcg
    22321 tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag aaaagttctc gttcggagga
    22381 aatccttatt aaaaccttaa aaagaagagt aatggaatta gaaaaagaaa ataaaaaatt
    22441 acagaaccaa attcaaaaat tatatggaga tctgtataat aaagaataat tattaatctg
    22501 tagacaaatt gtgaaaggat gtacttaaac gctaacggtc agctttattg aacagtcatt
    22561 taggtatatg tccaatctag ggtaagtaaa ttgagtatca atataaactt tatatgaaca
    22621 taatcagtta cgaggtgaaa tcatgagcaa tttgattaac ggaaaaatac caaatcaagc
    22681 gattcaaaca ttaaaaatcg taaaagattt atttggaagt tcaatagttg gagtatatct
    22741 atttggttca gcagtaaatg gtggtttacg cattaacagc gatgtagatg ttctagtcgt
    22801 cgtgaatcat agtttacctc aattaactcg aaaaaaacta acagaaagac taatgactat
    22861 atcaggaaag attggaaata cggattctgt tagaccactt gaagttacgg ttataaatag
    22921 gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa tttatatacg gtgagtggct
    22981 caggggtgaa tttgagaatg gacaaattca ggaaccaagc tatgatcctg atttggctat
    23041 tgttttagca caagcaagaa agaatagtat ttctctattt ggtcctgatt cttcaagtat
    23101 acttgtctcc gtacctttga cagatattcg aagagcaatt aaggattctt tgccagaact
    23161 aattgagggg ataaaaggtg atgagcgtaa tgtaatttta accctagctc gaatgtggca
    23221 aacagtgact actggtgaaa ttacctcgaa agatgtcgct gcagaatggg ctatacctct
    23281 tttacctaaa gagcatgtaa ctttactgga tatagctaga aaaggctatc ggggagagtg
    23341 tgatgataag tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt atatgaaaaa
    23401 ttctatagaa acttctctca attaggctaa ttttattgca ataacaggtg cttactttta
    23461 aaactactga tttattgata aatattgaac aatttttggg aagaataaag cgtcctcttg
    23521 tgaaattaga gaacgcttta ttactttaat ttagtgaaac aatttgtaac tattgaaaat
    23581 agaaagaaat tgttccttcg atagtttatt aatattagtg acatttgcat gcttcaaagc
    23641 ctgtcggaat tggtttttag tgaaaagaac acgatattca cggtttaccc acttataaac
    23701 aaaagatcga tactttttgt agtccttctt tgaaatcaat ggttgatgtc gttcaagaac
    23761 aatcaataca gagtctacac ttggcttagg atgaaaatat agtggtggta cttttttgag
    23821 catttttata tccatctcca ccattaatag taaacccaaa gctcgttgca gattttgcaa
    23881 tcttttcgca aatcccttct caacgataag atagctatat ttagcctgac tttcaaaggt
    23941 aattcttttg acaatatccg tactgatgtt ataaggaata ttaccatata tcttatagtt
    24001 tatatgtttt gggaaggaaa attttagaat atccgtttga atcactttta tattctcaga
    24061 ggggtttacc gcttctttag tcacttgaca taagcctcca tcaatttcta tagcagtaac
    24121 tgatcgactc attttgacta gctctttggt aaaatgtcct tttcctgatc cgatttctat
    24181 tacgttgtct tgtttactga tattcgtgtg attcaatatt tcttttacat gctttttaga
    24241 agtaataaaa ttttgcgtgt ctttagggtt tttctggttc attataacct tctccttact
    24301 ggttataatg aactgacatt aagcgttcat tataaccaat taattttgat ttggttgata
    24361 acgaacttta ttaataacaa atatagaaaa agtacccata cttttatctc ctttccattt
    24421 ttttcattat gaatgagata aagtaatttc tactactgcg atactggtgc acataattga
    24481 aacctcctta tgtaactgaa tatgattgta tcatcttttc attttagaat aaacctttat
    24541 atgtaatttt gcacttgaca cttcagatgg acgttttctt caaaataata tgttttgaaa
    24601 cttgataaga gaagccactg tctttactaa aaagtaaagg ctgcgataaa ggacagtgac
    24661 tttaataaag ttgcactgtt gtattgtctt tggataaaat tgtctaaagg gaagttttat
    24721 ccccaacgaa aaaacatcta aagtggtaag taatgaaaga atgtcgataa agtaggaatg
    24781 ggggatcttt ttgaatacaa tagaatcaca gtttgataag gttgcagaag attacgattt
    24841 cgtgaatgag cttttgaatg attattcatt ctttgtgtct aatatgtctc caaagaaagg
    24901 cagagcatta gatatcggat gtggctcggg tttgttagtg gagaaattag caagttatta
    24961 tgatgaagtg gtagggattg atatttctaa tcaaatgctc gatcttgcca aatctaaacg
    25021 tcaactaaca aatacggtct atctgaatat gaatgcagaa caacttaatt ttaatgagaa
    25081 gtttgatttt attgtaagcc gaacaacctt tcatcatttg gatgatatag ccagcgtaat
    25141 acagcaaatg aaggaactgt tgaatgagga aggaagaata gttattcttg ataatgtatc
    25201 tgaagtcgag acaccgccta cctatgttta taaattaggg gcaatccaag agtttttacc
    25261 gcattgtttc aaatttggaa taaaaaatgc gattagaatt tataatcaca acacatctaa
    25321 atcatggctt gagcatttag cttcagataa gtacctatct gaacaaaatt attacgattt
    25381 atatgaaaag ttgttgcctg gatgccaatt ccataaaatg ggttgggcaa tgggggttgt
    25441 ctggacaaaa taacttaaag ctgtggcttt gaaaagttga atttgtgatt gttttcagag
    25501 ctatcgggtg ctttagtata aataacaatg aataaaaaat cagcctgtat cttatgttac
    25561 tattaagtaa tttaagatac aggttttact atttttgaaa taagtacctt ccacttcaaa
    25621 tacatgtatt caaagtgaaa gtattgattt aaatgattct tttgtaattt actcttgata
    25681 atatgaaatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga
    25741 aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt
    25801 gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa
    25861 gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct
    25921 atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt
    25981 atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt
    26041 gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca
    26101 ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct
    26161 gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt
    26221 tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc
    26281 aggtattttt ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt
    26341 atcaaggaat aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata
    26401 ttcaggacaa ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca
    26461 ttggcaaaat cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag
    26521 tcatcgcttc tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag
    26581 cgtggaataa gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa
    26641 atccacgaga gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg
    26701 cgacagctaa tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac
    26761 caatatcacc gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag
    26821 gacgcgcaga atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt
    26881 ctgcaggaac acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc
    26941 cgtggacagg aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt
    27001 gtccaccgtg accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag
    27061 cttcagacag caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac
    27121 ttgaaaagat cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga
    27181 tgcggctcaa cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat
    27241 tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat
    27301 atcctaaacg ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt
    27361 tacgaccagt tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa
    27421 agctggcaaa aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa
    27481 tcgttttttc tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc
    27541 acaagactcc ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag
    27601 gagtgaaatc gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca
    27661 cgatccctaa tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc
    27721 taaaacggat gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat
    27781 gctcatctaa tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat
    27841 ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca
    27901 ccaaggcttt tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt
    27961 caattccaag taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat
    28021 cttggaattg taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga
    28081 cgcctgtctc gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca
    28141 gcattttctt tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg
    28201 tatggggtat tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa
    28261 agagattttg tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta
    28321 tagatcattg ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt
    28381 tagacaacac aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac
    28441 tggattcgag tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac
    28501 cgtaccttct gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa
    28561 taagtataga aatcaactag attgaaagaa aaaatcaatg tttatcatcc cgctataatc
    28621 ataatgactt ttttatgaga gaagtgttct attaataggc tcgttgtgct atcattttta
    28681 gtaatgaaaa tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa tgtggcatta
    28741 taccatgcgt tataagaaat tcttgttttt caatttcata tgcgtatttg gttttatttt
    28801 gattgaatta ggacttccta cgattttagc acgtatgatc gatgtaggaa tccgcaatga
    28861 tgattttgat tatgtgaagc aacaagggat atttatgatc gttattactg taatcggtat
    28921 tattttgaat atcttgttag gttattttgg ttcgaggatt acgactaata tcgtagcaga
    28981 tattcgagat gatttgttcg aaaagatcca aagttattcg catcaggaat acgaaaatct
    29041 aggcgtatct tcattgatca ctcgtacaac aaacgatgct tatcagatca tgttgttcct
    29101 gcaaaatatt ttacgtatcg gttttatggc accaatgatg ttcattgtca gtttgtacat
    29161 ggtcatgcgt acaagtgtga cactcagttt gtatgtagta ggtgcattac ctttactatt
    29221 attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa
    29281 cttagataaa atcaacagta tcttgcggga aaatctttca ggattgcgtg tgattcgtgc
    29341 attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga attatgcttc
    29401 tagttcaaaa agcttattcc gtttgatggc tgttgcacaa ccgggttttt atttcttatt
    29461 taatatcgta atggtattga tcatctggag cggggcagtg caaatcgatg caggcgagct
    29521 tcgtgtagga gatttgattg cgtttatcga gtatatattc catgccttgt tctcattcat
    29581 gttatttgct agtgtgttca tgatgtatcc aagagcagct gtttcagctg gacgtatcca
    29641 agaagcccta gatgccaaac cttcgattgt agaagatcca aatggtatat ctgatacaaa
    29701 aactaaaggg tatatcgaat ttcaaaatgt aacatttgct tatccaggcc attcgcaaga
    29761 gccagtgata cgaaatgtca gttttacagc ctctccagga gagaccgttg cttttattgg
    29821 aagtaccggt agcggaaaat ctacgttgat ccaattgatc cctcgctttt atgatgtgag
    29881 tgaaggaaag atcttgatcg atggagtaga cgtgagagat tatcggctaa gcaagttgcg
    29941 agataagatt gggtttattc cgcaaaaagc gctgctattt actggaacga ttgcagataa
    30001 tttgcgatac gggaaagaag atgctacgca agaagaaatg gaacgtgcag ctgagattgc
    30061 tcaagcttca gaattcattt ctcgaaaacc agatggattt aatgaacatt tatcagaagg
    30121 tggttccaat ttttcaggtg gacaaaaaca acgattggca atagccagag ccatcatacg
    30181 ccgtccggaa atctatattt ttgatgacag cttttctgca ttagattatc aaacggatgc
    30241 gaagttacgt gctcgtctga aaaaagaaac gacagaatct actgtattga tcgtagcaca
    30301 acgtgtggga acgatcatgc atgcagataa gatcatcgta ttaaatgaag gagaagtcgt
    30361 cggtatgggg acgcatcggg agttgttgga aaaatgcccg atttattatg atattgccgc
    30421 ttctcaatta tcaaaggagg aattagcatg aaaaaaacct tttcttcctt aaaacgttta
    30481 gctcgatata tcagaccgta tcgtgagact tttcttttag tgatcctatt tacagttctg
    30541 acagtggctt tcaacgtggc tttgccatat gttacaggac ttcctactac agaaatcagc
    30601 cgtaatattg ccaatggcga atcaatcaat ttttcttata tattcaaatg tctcatttgg
    30661 atcacagttg ttggaatagg gtattgtatt tcgcaattac tttctggtgt tttgatgaca
    30721 aatgtcgttc aaagtgccat gcatgatttg agaagagaca tagaagaaaa aatcaatcgt
    30781 cttccggttt catatttcga taaaaaccag caaggaaata ttttgtctcg tgtgacgaat
    30841 gatgtagatg cagtcagcgg tgctttacaa caagctttta ttggtatcgt aaatgcgata
    30901 ttgggaatta cgttggctgc agcaatgatg ttttatatcc agccgatgat ggcgctgatc
    30961 tctatgatca tgattccaac atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag
    31021 aaatattttc aaagcatgca gaattctctt ggtgagctaa acggctacgt ccaagaaaat
    31081 atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc
    31141 aaaaaagtta atcacagctt gaaatacttt gggtttcgtg cagcattcat ttctggcttg
    31201 atgatgcctc tagttcaatt aactgcctat gctacttata ttggtatggc tgttctagga
    31261 agcttttatg ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt catccaatat
    31321 atttggcaaa tcagtcagcc aatggggaac gtgacacaat tgtcttcagt tttgcaaagt
    31381 gcatcagcgg caacgaaacg tgtattcgag attttagatg agccagaaga aaaagaaaac
    31441 aaagtagatg cttcactgcc agaaacgatt ttaggagatg tgacattcga tcatgtcgat
    31501 tttgcatatg atccgaagaa accattaatc caagatttga attttgaagt gaaagctggt
    31561 caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga cgacattgat caatctattg
    31621 atgcgttttt atgacgtaga caaaggagca ataaggattg atggaatcga caccaaatca
    31681 atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc atggctttat
    31741 caaggaacga ttgcagataa tatccgcttc ggaaagcttg atgcgacaga ttatgaagta
    31801 gttgatgctg cgaaaacagc aaacgtagat cattttatcc gaacgatgcc agatggatat
    31861 gatatggcca tcaattctga gggagacaat gtgtctttag gacaaaaaca gttgctgacg
    31921 attgcccgag cagtggtctc cgatcctaaa atcttgattt tagatgaagc aacaagttca
    31981 gtagacaccc gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg
    32041 acaagtttcg tcatcgctca ccgtttatcg acaatccgtg atgcagatct gattctagtg
    32101 atggatcaag gtcagatcat tgaaaaagga acgcatgaga gtttattggc acaaggtgga
    32161 ttttatgaaa aactgtataa cagccaattt gctgaggaag cagaataagt catataagaa
    32221 taaaacagtt taaaacttat tttttgttct ctattcttga taatggtaaa gtaaagttat
    32281 caagaaaagt gaggacacgg agatggaaca gtttttaaat gaaaaaatta ctgaagccaa
    32341 aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt cagctgattt
    32401 ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt acgaattaat
    32461 tttaaaaaaa gtaatagaag aaaatataga tgtacaataa aaacaggtaa aacgtcagtt
    32521 accagatact gtaaatagac atctggcctg agcaggtttt acctgttttt tttcttatga
    32581 aataaatata tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg
    32641 agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca
    32701 ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata
    32761 aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca
    32821 cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca
    32881 atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt
    32941 gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat
    33001 gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa
    33061 aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa
    33121 ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt
    33181 tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag
    33241 attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt
    33301 gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc
    33361 cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg
    33421 caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct
    33481 cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca
    33541 ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt
    33601 cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa agatagatat
    33661 ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat
    33721 catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat
    33781 cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag
    33841 agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa
    33901 tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat
    33961 aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata
    34021 gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg
    34081 atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa
    34141 gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa
    34201 cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg
    34261 attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat
    34321 tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat
    34381 gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt
    34441 acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc
    34501 cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg
    34561 ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg
    34621 gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa
    34681 aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat
    34741 gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg
    34801 actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca
    34861 gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta
    34921 ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag
    34981 tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct
    35041 tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa
    35101 ccggagatac agctggactt attgatgagt acttctgata aaaagatcca actagcagca
    35161 gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc
    35221 caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg
    35281 gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt
    35341 cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt
    35401 ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag
    35461 atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc
    35521 aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa
    35581 cgttactcag ccgctgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac
    35641 cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat
    35701 gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg
    35761 ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca
    35821 gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta
    35881 gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac
    35941 ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc
    36001 gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa
    36061 gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa
    36121 acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag
    36181 ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat
    36241 caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga
    36301 aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat
    36361 ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca
    36421 aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt
    36481 tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag
    36541 acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat
    36601 cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc
    36661 tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg
    36721 cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat
    36781 acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg
    36841 caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa
    36901 tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca
    36961 attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat
    37021 tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt
    37081 ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag
    37141 ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat
    37201 gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt
    37261 cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc
    37321 gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt
    37381 gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga
    37441 agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa
    37501 atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DAZBTH010000017        37247 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000017 DAZBTH010000000
VERSION     DAZBTH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.017"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..841
                     /locus_tag="ACHGNW_000843"
     CDS             <1..841
                     /locus_tag="ACHGNW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6925179.1"
                     /translation="AYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGG
                     VVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQA
                     PTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQD
                     ANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAI
                     FLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKK
                     RNI"
     gene            complement(1194..2042)
                     /locus_tag="ACHGNW_000844"
                     /pseudo
     CDS             complement(1194..2042)
                     /locus_tag="ACHGNW_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2195..3307
                     /locus_tag="ACHGNW_000845"
     CDS             2195..3307
                     /locus_tag="ACHGNW_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6925180.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3385..3678)
                     /locus_tag="ACHGNW_000846"
     CDS             complement(3385..3678)
                     /locus_tag="ACHGNW_000846"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN6925181.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3846..4160
                     /locus_tag="ACHGNW_000847"
     CDS             3846..4160
                     /locus_tag="ACHGNW_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN6925182.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4181..6160
                     /locus_tag="ACHGNW_000848"
     CDS             4181..6160
                     /locus_tag="ACHGNW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6925183.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6173..6499
                     /locus_tag="ACHGNW_000849"
     CDS             6173..6499
                     /locus_tag="ACHGNW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN6925184.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6515..7033
                     /locus_tag="ACHGNW_000850"
     CDS             6515..7033
                     /locus_tag="ACHGNW_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925185.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7033..8385
                     /gene="celB"
                     /locus_tag="ACHGNW_000851"
     CDS             7033..8385
                     /gene="celB"
                     /locus_tag="ACHGNW_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6925186.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8469..9974)
                     /locus_tag="ACHGNW_000852"
     CDS             complement(8469..9974)
                     /locus_tag="ACHGNW_000852"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN6925187.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10066..10656)
                     /locus_tag="ACHGNW_000853"
     CDS             complement(10066..10656)
                     /locus_tag="ACHGNW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6925188.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10835..12481
                     /locus_tag="ACHGNW_000854"
     CDS             10835..12481
                     /locus_tag="ACHGNW_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFN6925189.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12530..13249
                     /locus_tag="ACHGNW_000855"
     CDS             12530..13249
                     /locus_tag="ACHGNW_000855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6925190.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13728..14675
                     /locus_tag="ACHGNW_000856"
     CDS             13728..14675
                     /locus_tag="ACHGNW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925191.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14687..15607
                     /locus_tag="ACHGNW_000857"
     CDS             14687..15607
                     /locus_tag="ACHGNW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925192.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15648..17105
                     /locus_tag="ACHGNW_000858"
     CDS             15648..17105
                     /locus_tag="ACHGNW_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6925193.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17450..18064
                     /locus_tag="ACHGNW_000859"
     CDS             17450..18064
                     /locus_tag="ACHGNW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6925194.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18057..19781
                     /locus_tag="ACHGNW_000860"
     CDS             18057..19781
                     /locus_tag="ACHGNW_000860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6925195.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19778..21229
                     /locus_tag="ACHGNW_000861"
     CDS             19778..21229
                     /locus_tag="ACHGNW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6925196.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21702..23855)
                     /locus_tag="ACHGNW_000862"
     CDS             complement(21702..23855)
                     /locus_tag="ACHGNW_000862"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN6925197.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23857..24903)
                     /locus_tag="ACHGNW_000863"
     CDS             complement(23857..24903)
                     /locus_tag="ACHGNW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6925198.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25053..26342
                     /locus_tag="ACHGNW_000864"
     CDS             25053..26342
                     /locus_tag="ACHGNW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN6925199.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26355..29057
                     /locus_tag="ACHGNW_000865"
     CDS             26355..29057
                     /locus_tag="ACHGNW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6925200.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29042..29899
                     /locus_tag="ACHGNW_000866"
     CDS             29042..29899
                     /locus_tag="ACHGNW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6925201.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29970..30827)
                     /locus_tag="ACHGNW_000867"
     CDS             complement(29970..30827)
                     /locus_tag="ACHGNW_000867"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6925202.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31038..33209
                     /locus_tag="ACHGNW_000868"
     CDS             31038..33209
                     /locus_tag="ACHGNW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6925203.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33277..33618
                     /locus_tag="ACHGNW_000869"
     CDS             33277..33618
                     /locus_tag="ACHGNW_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6925204.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33894..35648)
                     /locus_tag="ACHGNW_000870"
     CDS             complement(33894..35648)
                     /locus_tag="ACHGNW_000870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925205.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35659..36495)
                     /locus_tag="ACHGNW_000871"
     CDS             complement(35659..36495)
                     /locus_tag="ACHGNW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6925206.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36497..37180)
                     /locus_tag="ACHGNW_000872"
     CDS             complement(36497..37180)
                     /locus_tag="ACHGNW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN6925207.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12017 a         6551 c         7640 g        11039 t
ORIGIN      
        1 agcatatcag ctggtagaaa ctcaagcgcc aacggggtat gaattagatg cgacaccgat
       61 cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaaccgact
      121 tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaag tcctgcaagg
      181 agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg
      241 agcagatgga aaactggcga ttgacggctt agcaccagga gtgtatcagc tggtagaaac
      301 ccaagcgcca acggggtatg aattggatgc gacaccaatc gagtttgaga tcgaaagaag
      361 tcagacagcg gtggtagaat tgacgaaaga aaatcgactt acaccaggcg gtgtcgtgtt
      421 gacaaagatc gatgaccaaa gcggagaagt cctgcaagga gctgtttttg agttacaaga
      481 tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa aactggcgat
      541 agatggctta gcaccaggag catatcagct ggtagaaact caagcgccaa cggggtatga
      601 attggatgcg acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg cgatcttttt
      661 aacaaaagag aatagaaaag ttgctgatag ctcagatata agaggaaaca atccaacaag
      721 cagtggaaat aagcatcttc caaaaactgg agaaaccatc attagccagt ttatattgtc
      781 aatattgggt gtgctacttg ttttcatcag tataaaattt agaaagaaac gaaatatatg
      841 agctaaaata aaaaattgaa taagcatagt ttttaaaatc aactagtacc catcaattga
      901 atggaaacta ccgattttag aaggggtata caaacaaata ttagtatcgg agtagagaag
      961 gttgaaccca accagtaaat aagtaaagac aattttgtgc tgcgttgaga agtgaaaaat
     1021 aaacctttgt agtaatacta gttaataaaa aatatacaaa aacactgcga aaatcattct
     1081 tgagtttcgc agtgtttttt gcctaatgac tcaagtttat tgggtaatac tttgattttc
     1141 tttctacaaa taatcagcga tcagttgtaa ttttttctct agttttaatg gctctttgtc
     1201 aataaggaga aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa ctcgtgaata
     1261 aaggttacgc ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc accaatttcg
     1321 catttggtag aacatgtttg gtgagctgaa agtaggtggc gttcatgtct gtaactaaga
     1381 attctatttc tttaggatta gtgcagctaa ggaaatagtt tgttaatcga ggaatttttt
     1441 gtgtaggtaa attttctcga ttaatttttc tgtctcgcca tccgcggaaa taaaactcag
     1501 tttatcctca acggaaacgt gagaacgaaa ttcacaaccc tcaacactca agacagaatt
     1561 cttttggaag actttggtaa atagcttctc aactctttca atgtgcgaat aacggttgtc
     1621 aaagatacct aacaactttt cgaaataaaa aataacgaca ctttttcagt cagtaaagaa
     1681 gtgattttat gtctgacatg atttgaaatc gagtgccttg gacggataaa ataactttga
     1741 gcgttccaat ggcttcgaca gtttttacag gtatagcgct gcttttttag gcgtataacc
     1801 attggcatat gattatatta ttcaaagtgg acaatggttt ccttcttctt gtttttcacc
     1861 accattaatt tcctatttcc atctaccaca gcagaaccac aatttctaca gacacatgga
     1921 gcagggaaga gaactgcagc aacgatcaat gtttttttct tctgtaaggt ttcgtaagag
     1981 acctctgtaa tcatcaaatt tttgtctatt gatctcagaa tttttttgat agaaccattc
     2041 ataaagcata tcgttctctc agttgtttat tttgtggtga tttaatcata caagaaaacg
     2101 atatgttttt ttataaaaac atgaatagga aaaatagttg acaagaaaat gacaacgcca
     2161 tatgatgttc catagtattt acaacctgtc atagatgact gtaaaaaata aaagtaaaaa
     2221 gaaaggacga caacaggtgg atttttatca gtctctacaa ctagatccgt ttattttaaa
     2281 acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc atgtatattt gttcattgtt
     2341 tttacgatca ttattcatcg tattgtttgc catttgtttt atcattataa ttacaacatt
     2401 atttgaatcg aagcataaac catacgctgt ggttttattc tgtatgttga tgagtattcg
     2461 ttttgttgac tttggttata agatttccca ttcgatcata ggtttagcaa tcgtcatgtt
     2521 ctcactatta gtagcaccgt atgtccagct gatcaaatgg tcagttatgg gcatgttgat
     2581 tcattttatt ttactaagca gcatattaat ggcaaccgct agtgatccca aaatggggaa
     2641 tgcttctttg tacggctttt catatctctt tattgtttat tcgttaccta aagattcgtt
     2701 gaacaagaat ttctttacac aaacaagtag cttattattt ttgttttttt gttggttttc
     2761 agttctcctt tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat
     2821 ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg catttggcat
     2881 tagtcttttg atcgttgcag gagagtatgt cccttttcag cgattgatgt gggcagggtt
     2941 tgcattttct tctattgtat ctagctatgg attgatgtcg attggtttca aagaacgagc
     3001 agtagaccga atcattggct cattgattgg atgtgtttta tttattggta tcagccaatt
     3061 tattcctttt aactgggtcg gtatattagg tggtctagct ttaggtgttt gttctaccta
     3121 tagatataaa acagttttta attcttttgg tgctttggca atcactgcca gtttattcgg
     3181 ggttccagga gcagtaacga tccgcatttt tgagaatatc cttggggtat gtttaggaat
     3241 catgtatatt ggggtgactg aaatacttat ccgaaaaata agagaaaaac acggtttgaa
     3301 tcattagatg agatcgaata aaaaataagg aaaaagtgaa gatacacatg gatgataaat
     3361 tgctatcatt ccatgtgtat ctttttacgg taaatcagtg aaacgttcga ctttcatttt
     3421 taatttatac tttttacgga gttcgtttat ttgagccatc atttcggatt tgtgatgctt
     3481 ttcgattgct tcttcatctt tccaacggtc gatcagtaaa agagattctg gatcttctag
     3541 tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt
     3601 tctttcgacc atttcttctg caaattttct ggcttgtcca tttgtacttg tatacaaaat
     3661 attcactgtg atactcacta aatcccctcc tgaaaaaatt ctatcactag tatatcacgg
     3721 tgccagaaat tattattcaa gatatgatac acattttttc cgcaatgcta cggaaatata
     3781 tattttgaat agaaaacgct tacaagatat gcttggttta taaaaataaa aggagtgatt
     3841 gatttatgaa aaaagcattg attatttgtg cggcaggaat gtcatcctcg atgatggcct
     3901 caaagacaac ggatttcttt aagcaaaaaa gagaagaggt actcgtagat gcagtttcag
     3961 ctacagaagg cgataatatg attaagaata gtgattttga tttatttctg atcagcccgc
     4021 aaacgacgat gtttttggat aaattcaaaa aattaggaga atctgttgga aagcctgttg
     4081 ttagtatccc tttccaagcg tatgtcccaa tccctacagg catccagaag ttagcagagg
     4141 tgatcgaaga gaacatatga tggaatagga gtggatgata atgaaacaga aggaaaaatt
     4201 gctgttgaga tatttgattg atcacaagaa ggagtatgtc accagtcaac gtttagcttc
     4261 tgaattgtta ttatctgatc ggacaatccg taattacttg cagcggatca aagagttagt
     4321 agaaaaaaac ggaggtaaga tcattgctaa accaggttat ggctaccaac tgcatatttt
     4381 acaaagactg acatttgact tgtttttgag tcggcaagaa atagtacctg gatatgctag
     4441 agaaccgcaa ggattttatg agtccgaaga tcgacaaaag tatatattga accaattgct
     4501 tttggaagat gccattcttt ttatggatga tttggcagaa gagctttata tcagtcgttc
     4561 cagtttgaca aaggacatgc aagaaatcaa ggagcgattg attccatatt cactaaaaat
     4621 cgtttctaag catggtcaag ggacatggat cgaaggggaa gaaagaaatc gacggcattt
     4681 tatcatggat actttttttg gaaaccatta tggaaattca ttgaaagaat atttagggca
     4741 aagccgtttt ttcccagaca tcagttttga agagctagtc attatcattc tcgatgaaac
     4801 aagagaagct aagttaaaag tttcggattt tatcatacag aatctgactt tacacttggc
     4861 tttggggatc aaacggctcc gagaagggtt tgaaatcaag gaattaggca tcgaacaaga
     4921 agtttctaag cgagtagagt atcaagtggc gcagcggatc gttcaacgta ttgaggtaat
     4981 cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg acattacatc tcatggctaa
     5041 gtcgaatcac aaagaaaatc aaaatgatca ggaactatca aaggaattag cgaaagttct
     5101 gaaaagattg tctcaggaat ttcatttttc attagtagag gatctccagc ttttcaatgg
     5161 actactcgat catttgtcac cgatgatgat tcgattgagc cgaggtattc gcattgagaa
     5221 cccattgact gaggagataa agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca
     5281 tttcagcaac atgccggctt tgaaaaacta taccatcaat gaagatgaat gggcctattt
     5341 ggctttgcat ctcatggcgg cattggaaaa agaaagagct gcgcataaac tacatgcatt
     5401 gattatctgt gcaacgggtt acggcagtgc ccaactatta aagaatcgcg tagtcagtga
     5461 attcggtaaa aatattacag tggtaagtgt aaaaggctat tatgagatca atgaaacaac
     5521 tttaaaagga atcgacctga ttatttcttc tattgatctt tcgacaatgt ttttcaaaat
     5581 tccagtttta catgtcagta tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt
     5641 agttggagaa tctataccct atcattcctc agatagaccg ataccagcat tttctctaca
     5701 gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta
     5761 cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa aagctgagca taaatgaaga
     5821 agaaggatat ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat
     5881 catattcagt gatacagtgg cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa
     5941 ggtagccgtt gcattgattc cttctgggat gcagtgggat caatatcaag aaattcattt
     6001 tgttttttta gtttcgcctt cttatgtcga aaatgaagga attacggtcg tgactaaagc
     6061 aatcgttagg ttagttgacc agctaaaagt acagcaggaa atcttagctg aacctatttt
     6121 tgagaatttc agcagtaaat ttatcaaact gatcgaataa ggaggaaaac agatggctga
     6181 aaaaataaac agtgaaacac tacaagtgac ttcttttgat atcattttcc atagcggaaa
     6241 tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt
     6301 agaagagaag ttgcgacaag caaatgaaga gattcttcat gcgcatcagt cacaaactca
     6361 acttctgaaa gaatatgcca gcgggacaaa aatagaaatg gaaattatca tggtccatgc
     6421 acaagatcat ctgatgacga cgatgacatt attggaagtt gcacaggaaa tgaattattt
     6481 gtatcaaaaa aataaataga aaggaaggat cattgtgcag gatacagtaa aaaggaaaat
     6541 cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt
     6601 acgttacctg actgcctttt tctttttcat gaacatgcaa tggatgattc tgcttctatc
     6661 agctaaaagt ttaggaagta gtttaccgat gattctgatt ttagctattt tgcctgcagt
     6721 aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga caaagtggta
     6781 ttttttgttc cagagtgtct ttaatttatt agtgattcca gtgttgttta cctcaggatt
     6841 tacattcttg tatcctttta tggcaaataa caatggagga cagttatttg ttttcattct
     6901 tgttgtaagc ggtatttttg ccagtgcatt tatacagtat cgtttaaaga aaatttcact
     6961 caaccaagac caacagtata tccgtattaa acaatatgaa aaagcgttat atttaggaaa
     7021 ggagaataac taatgtttga ttttttgcag aaatacctga tgggacctat ggggaaaatt
     7081 gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga tggcttctat tcctttcacg
     7141 attgtaggct cgatgttttt ggttttaaat attttgccat taccgttccc atttttagaa
     7201 ggatttttta atgcaacttt tttcaaagtt agtgacttgt acatgatcgt caatacaatg
     7261 acgatgggga ttttatctat ttactttgcc attgtgtttg cttatgaatt aacgtcaatt
     7321 gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg gggcattact atctgttttt
     7381 gcattcttta tgtgtatccc tgaattgatc atgactgatg gtaaaatcag cttgattgct
     7441 tctatcaatg agacagaaac tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt
     7501 ttaggaactt caggaatctt tacagctatt attatgtcga ttattgcggt cgaattgtat
     7561 tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag atgttgttcc gccaggagtt
     7621 tctcgttctt tcactgctct gatcccaaca tttgtcattg cgtttgtggt gatgatcatc
     7681 aatggattat tagttgcact aggaacggat atcttcaaaa tgattgctat cccatttggg
     7741 tttgttacgc agttgacgaa tacatggctt ggtgtcatgg tgatttattt cttgatccat
     7801 gcattgtgga tcgtagggat ccatggagcg aatatcatca cttctttctt gaccccgatc
     7861 gtattagcta atatggcagc taacgcacaa ggcgcaaatt atccgttggc aggagaattc
     7921 aacaattctt atgtaaccgt tggtggatca ggtgcgactt taggattgat catctttatt
     7981 gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa aagcctcact tgtaccagga
     8041 ttattcaata tcaatgaacc aatcattttc gggatgccaa tcgtctacaa tccctattta
     8101 gcggttccgt ttttcttagc tccgatggct tctgctacac tcgcttattt tgccattcgg
     8161 ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc cttcaccagt cgggatcgga
     8221 gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc
     8281 atcgcattca ttatctggtt cccatttatc aaattctatg ataataaatt attaaatgaa
     8341 gaaagagaaa aggcagcaga attagctagt gaaaagttaa aatgatttct aagaaaaaag
     8401 gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc actagtatat
     8461 aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc ataaggttca
     8521 tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac gactgaatct
     8581 tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat ttcataatga
     8641 acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt tttcggatct
     8701 tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt
     8761 ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac aacaattaca
     8821 cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct tagataatta
     8881 tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc cattcggcct
     8941 ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc tgttagtgct
     9001 gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag caaaatatca
     9061 taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa accgtgagta
     9121 cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata agtgatcgtt
     9181 ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact ttcatgattt
     9241 aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata gtcatcaaat
     9301 gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat tccgacatct
     9361 aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc catcactaga
     9421 tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa tgactcaggc
     9481 gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat agtagaaagc
     9541 aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt
     9601 gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg atagtcgtaa
     9661 aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat tcctactgct
     9721 gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat tgctgaatcc
     9781 aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc atcagctttt
     9841 cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag atgccagcct
     9901 cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg gatgtcttct
     9961 aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta ttatagcaca
    10021 ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt caaattttct
    10081 tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg taatagatag
    10141 gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga cgataagact
    10201 attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga aaataatcgg
    10261 cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact
    10321 gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg
    10381 aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa aaaatactga
    10441 ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc aaacagtaac
    10501 tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca ctccggcgga
    10561 agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga aaagacctaa
    10621 aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata aatgttagcc
    10681 ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa
    10741 ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca attaaaatga
    10801 tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa aaccagcatt
    10861 agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga ccgccagtaa
    10921 tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt ggatgggcga
    10981 aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg cattgtttct
    11041 actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac gctataattc
    11101 attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga tgatgggcgg
    11161 gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag ctcatgcttc
    11221 tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg tattggtttt
    11281 tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga tgccttatgc
    11341 gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg ctacattcat
    11401 catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc
    11461 atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga aacatcaagc
    11521 gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa cgaaggaatt
    11581 gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag gggtaacgat
    11641 cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca ccgagtattc
    11701 caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata agttgacgat
    11761 ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga ttacagaagc
    11821 tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt tacagttgtt
    11881 ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc cgctaaatac
    11941 attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa gttttgtagg
    12001 actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt ttgaaaacaa
    12061 ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc aatacccagc
    12121 cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat ttgctgtgtc
    12181 ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa gttttgtctg
    12241 gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt cgtggatcac
    12301 aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc tattgttgat
    12361 tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga aaatccgctt
    12421 ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa aaataaaata
    12481 agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga
    12541 taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa
    12601 aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt
    12661 cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt tttactacat
    12721 tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc aaaccgtaat
    12781 cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc gattagataa
    12841 agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc agttactatc
    12901 acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa tgacagaaga
    12961 agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga cattgccggc
    13021 aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc gtctgatttt
    13081 gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt
    13141 atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga ataaagggca
    13201 atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat tgaatggata
    13261 gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg tccgtctgac
    13321 tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
    13381 cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat tgttccttgt
    13441 gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa acgctgaatt
    13501 cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
    13561 aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa acgctgattt
    13621 cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta ttctcattat
    13681 tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa
    13741 aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc ctgatggctg
    13801 taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg aatgtcgtag
    13861 cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg gaaagtcctt
    13921 gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt ctcatcacta
    13981 aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc tcagtcgtat
    14041 ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat caaacgatgt
    14101 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
    14161 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
    14221 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
    14281 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
    14341 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
    14401 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
    14461 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
    14521 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
    14581 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
    14641 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
    14701 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
    14761 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
    14821 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
    14881 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
    14941 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
    15001 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat
    15061 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
    15121 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
    15181 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
    15241 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
    15301 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
    15361 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
    15421 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
    15481 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
    15541 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
    15601 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
    15661 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
    15721 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
    15781 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
    15841 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
    15901 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
    15961 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
    16021 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
    16081 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
    16141 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
    16201 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
    16261 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
    16321 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
    16381 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
    16441 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
    16501 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
    16561 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
    16621 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
    16681 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
    16741 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
    16801 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
    16861 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
    16921 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
    16981 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
    17041 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
    17101 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
    17161 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
    17221 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
    17281 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
    17341 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
    17401 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
    17461 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
    17521 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
    17581 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
    17641 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
    17701 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
    17761 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
    17821 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
    17881 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
    17941 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
    18001 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
    18061 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
    18121 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
    18181 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
    18241 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
    18301 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
    18361 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
    18421 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
    18481 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
    18541 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
    18601 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
    18661 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
    18721 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
    18781 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
    18841 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
    18901 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
    18961 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
    19021 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
    19081 gggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
    19141 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
    19201 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
    19261 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
    19321 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
    19381 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
    19441 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
    19501 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
    19561 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
    19621 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
    19681 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
    19741 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
    19801 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
    19861 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
    19921 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
    19981 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
    20041 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
    20101 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
    20161 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
    20221 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
    20281 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
    20341 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
    20401 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
    20461 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
    20521 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
    20581 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
    20641 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
    20701 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
    20761 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
    20821 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
    20881 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
    20941 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
    21001 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
    21061 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
    21121 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
    21181 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa agaaaaagcc
    21241 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
    21301 acttgaggca aggcagcttt ttcatgacct ctcgataaac ggagttcaaa agccagtttc
    21361 tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
    21421 atactcgaaa ctaaacagct ttttcacgac ctctccacta aacggtgttc aatcagctga
    21481 ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc
    21541 taatactcag gtcagaacgc tgatttcaca acctctcaat aaacggtgtt caaaagcttg
    21601 catctgcggt aataagacca accaaacgaa aaatatgagg atctattttt gcttggctgt
    21661 tcttattact tgatgcagga cagctttttc acaacctctt gttaattata gtttgataag
    21721 gaaaaaggca aatcttttgc ttgatatttc ttagggtgag ggatgatccc taattggtaa
    21781 gagatcgtgc cgcctttcag caaatcttga tgggtaaaat ataatttcgt atattctctg
    21841 tccatccgtt tgattttatg gataaattgt tgctgcggcc gattaggcgt tgcttcaatc
    21901 gtcaattcct ttccatttga caaaagcaaa acagccttgt caattaatgg cataccgata
    21961 acatattcac cggttccagg cgtgacagga taaaatccca gactattgaa gatataccaa
    22021 gcggccatac ttccattgtc ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc
    22081 tgagtcatca actgtttaag cagtggctgt gccatttcag gttgtcctat ataactgaac
    22141 aagtaaggat agtgaaaact tggctggttt gaaatagcta attgtccaaa ttctatcgcg
    22201 gccatctcac tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc
    22261 tgattgcaca gctcaatgag tttttcttta tatctctcac tgccaccata ttttccgatc
    22321 agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc aagcacttcc ttctgcataa
    22381 tcttgtcccc aacgattagc tgaaaatggt tctctgaact tgccatcttt atcttttgaa
    22441 cgcataaatc ctgactcata gtcaaagata ttttgatagt tcaatgattg ctgctgatat
    22501 ttcttcgtca tttcctcttc ttctagtacc ttggcaactt ggctaataca aaaatcgcta
    22561 taagcatagt ctaatgtgtg attgactgat tcgtggtagt gattaggaac atagccatat
    22621 gtcaagtaat cttctgttcc ttggcgtcca taatttggat gtttgctttg aaccgtagca
    22681 cctttttcca ttgccgttag aaattcaggc atcaagtctc gtcggatatc ttttacagca
    22741 gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc atcaggagac
    22801 agccattttg gcaaatagcc actttctcta tagctgttca aaaaaccttc cagcatttct
    22861 tcgtattttt ctacagcaat caaagaataa agaggataaa ccgttttata cgtatcccaa
    22921 aacccattat ttgtatagag aacaccaggt tttatagatc gactagtcgt atcatagtgg
    22981 attttttgcc cactaggatc aatttcataa aatgtttgtg gaaataaaaa cacccgatat
    23041 aaattatgat aaaacgtccg caattgttgt tgatcatggt gtgtgagctg tatacgatca
    23101 aaataggact gccattgttc ttgactgtga gcaaggtaag tctctttgct ttggttttgt
    23161 tctagttcca ggttataagc tgcttgttcc gagctgataa aagatgtccc aaatcggata
    23221 gtttgttttt ttgtctgttc aaaatacaac gggatcgagt cgctattttt gttggaaaca
    23281 gctaactcat caattgttaa aggatattca aatctcaata taaaataaaa agaaaaatca
    23341 ggatcttctg aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg atctataaca
    23401 aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt gctgtaatca
    23461 atagataaga ttccgccata catgctaggt atcaatgtag aacagatgcc ataacgtaat
    23521 tgacagatcg acagatgagt cgggcaaaat atactttctt ctgggcggta agaactttga
    23581 gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag catatgactg
    23641 aaatcaccca tccaaggact tggttgatgg gtaagacggt agccttgaaa aacgcggtca
    23701 tcaggatgaa accaccagct gccgttttga tccgtagttt gcggtgcaaa ataattcatc
    23761 ccaaaaggaa cgccagtata aggctggcag ttcccattag agaaatttgg ctgattcgct
    23821 gttgcatgtc tagtatctat ttgaccgata ttcattttat ttctcctttg ttgattgtct
    23881 tttgatgaat gaaactggaa atttactatc ggatggtcga tcttttgtct ctaaccaagt
    23941 gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag
    24001 tggcgggtca acaagagctg ctgcttggat atcatcaaat ccgataattg aaaaatcttc
    24061 agggatatga taaccatgct gtctcaagag attcatggtc tggatagcga caacatcgtt
    24121 ttcacaaaca aaagcagtta cttcatactt agtgatataa gaaagcaatt catctaatgt
    24181 cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca gctttgtcca atgcttcaag
    24241 gtaaccaagg taacgctgtc gaacggattg tggatgagtc tgcgtaccaa acaaataagc
    24301 gatttttgaa tgtccctgtt cgatcaaata agaggcagca agcgcaccgc cttggaaatt
    24361 atcggaaaga acggtaggaa aaggtaactc atagattttt ttatctaata tgacgacagg
    24421 gaattctttc aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat caagagcata
    24481 ataaatcaat ccgtcaaatt cattgattag ctcttttgct gttttatttt gtagaaagtc
    24541 aagcgtcgtc atcatgacat caatctgttt atcttgcatc acagggttca atcccttgct
    24601 aaaatcacca acagacaaat cagcgacata tggaagaaga aacagaatcc gtcgtgtttt
    24661 tgtaggagaa gcaagaatct gttccggaga agcggattta acgaaactgc ctttcccgcg
    24721 aacgcgataa ataagtccta attgctctaa ttcagtcaat gcacgtttgg aagtgattct
    24781 gcttacttta taggtacgtg aaagttcttt ttcagttggt agttggctgt ctacaggtaa
    24841 ttcaccagtt aaaatagccg tttgcaaatc attaaatatt gtctggtaca atggttgagt
    24901 catataaaca cctcttaatg atatatcata ttcaaataat accataatgg atgaaaaagt
    24961 aaataaaaag atattttttg aaagcgtaga cataattggc tgtatgaagt ataatcttta
    25021 aagatatatc aaatagaaag aaggttttat tagtggttta taaagatata ccaaaatcgg
    25081 tgcagaaatt tatggaagat atcacacaaa aatgtgggga tatccatgcg gactgggcaa
    25141 agaattttac agcagcattt gccaatactc tgttgacaac agtcaaaaag caaaatgacg
    25201 gaaccacctt tttattgacg ggagacattc cagcgatgtg gttaagagac tctactgcac
    25261 aagttcgtcc ttatttagtg attgcaaaag aagatgagga attagctgac atgatcagcg
    25321 ggttagtcaa aaagcaattt ttttatatca atatcgatcc atatgcgaat gccttcaacg
    25381 aagaagctaa cggtgctggc caccaaacag atcatacaaa aatgaacgat tggatctggg
    25441 aaagaaaata tgaaattgat tctttatgct acccaataca gttggcctat cttctttaca
    25501 aaaatacagg aaaaactgat caattcaatc aatcttttat tgaaggtatc aaaaaaatct
    25561 tggacgtgtt caaaacagag caagatcatg aacactctcc ttatacattt gtacgagaca
    25621 cgacaagaaa agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag
    25681 gaatgacatg gtctggtttc cgtcctagcg atgatgcttg ccgttatgga tatctggtac
    25741 catccaatat gtttgcggtg gtcgtgttag gttatgtgga agaaattttt caaacgatac
    25801 tcgatgataa agaaattgta cagcaaacac agaaattgaa gaaagagatc caagagggga
    25861 ttcagaatca cggacgaacg gttaatcaaa aagggcaaac gatttatgca tatgaagtag
    25921 atggaatggg aaacgccaca gtcatggatg acagtaatgt gcccaatctg atcgccgcac
    25981 cttacttggg atattgttct gaacaagagg aagattatct gcaaacaaga agaacattgt
    26041 tgagtaaaga aaatccatat ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc
    26101 atacgccaga aaaatacgtt tggccgattg cattagctat ggaaggaatg acaacgtcag
    26161 acaaatctga aaaagagcgg atattgaatt tacttgtagc aactgacgca ggaacacacc
    26221 ttatgcacga aggattcgat gtgaacaatc ctgaaaatta tacgagagaa tggttttctt
    26281 gggcaaatat gatgttttgc gaattagtca tggattactt tgatattaga gtagaaaagt
    26341 aggaggtatc gtaaatgaaa aagaaagtat atatcatttc tcatagccac tgggatcgtg
    26401 aatggtacat ggcttacgaa cagcatcaca tgcgactgat tgaattgata gacgatctac
    26461 tagagctatt tgaagttgat ccgtccttca acagttttca tttggatggg caaacgatta
    26521 ttttagatga ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa caagcaatca
    26581 atgaaggaaa attgagaatt ggaccatttt atatcttgca agatgatttt cttatcagct
    26641 cggaatcgaa cgtgagaaat atgctgattg gcatggaaga aagccgtaaa tggggaaccc
    26701 ctgtcatgtt aggctatttt cccgatacat ttggaaatat gggacagacg cctcaattaa
    26761 tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca attggctttg
    26821 ataatcaagt tttggaagca gaaaactatt cttcccaata ctcagagatg tggtggaaag
    26881 gccctgatca aacagcaatc tttggtctgc tatttgccaa ttggtatagc aacgggaatg
    26941 agattccagt agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta gcggatgcag
    27001 aacaatatgc ctctacgaac cacttattga tgatgaatgg tgttgatcat caaccggtac
    27061 aaaaagatat atcaaaagca atccatctcg caaatgaact gtttccagat tacgagttta
    27121 ttcattctaa ttttactgac tatctggaag cagttcaaaa agatgtgcca gaagacttag
    27181 gctcagtaga aggagaattg acaagtcaag aaacggatgg ctggtatact ttagccaata
    27241 cagcatctgc tcgggtttac ttaaaacaat ggaataccaa ggtccagcga cagctagaaa
    27301 atattacaga accacttgcg acgatggctt atgaagttag tggaaactat ccccatgatc
    27361 aattagatta tgcttggaag acattaatgc aaaatcatcc tcacgacagt atttgcggtt
    27421 gttcagtcga ttctgtccat cgggaaatga tacctcgttt tgaaaaagca gatgaagttg
    27481 gaaagtatct tgcacaagat tccttagaac agctgaccgc agcaatcgat acgaccggat
    27541 tcccaaaaga cagctttcca tttgtcattg taaatactgc aggaatggat aaaactggtg
    27601 aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata cctgaacaac
    27661 tatatcaaga gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg
    27721 ccactatacc agctatttta tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag
    27781 accgtttccg tgtgccatat atggcaagaa tgatcaaagt gactttacca ttagaaaaca
    27841 tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa
    27901 aggaaacaat gattcatcag agtggacgaa tcatcgaaaa cggccctcta catttgacga
    27961 ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa caaacgaaag ttgaataatc
    28021 tgcatatttt tgaagatatc ggcgatatag gaaatgagta tatctttaaa caacctttct
    28081 gtgatcagcc aatcctatcc agtaacaaag aaaactctga agtaaaagta ttagtagata
    28141 caccggaaat agcaaaaata agtattctac aggaaatgga aatccctgtt tctgcagacg
    28201 agcgtttaga aaaagaacag cagatggttg tggaatttcg ttatcgaaaa gcagaacgat
    28261 ccaaagaaaa acgaatcctt caaataaaaa caatcatgac aatcagaaaa gatagtaaaa
    28321 aaatcgactt tgaaacgact ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt
    28381 ttcctacaaa gttacatgtg gaacatcacg aagcagatag tatttttgaa gtggtgaagc
    28441 gcccaaacca tgtgtctaaa agttgggaaa accccacgaa tccgcaacat cagcaagcat
    28501 ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg aaactttggt ttgaatgaat
    28561 atgaagtaac agaagacgga caaatcgcag tgacgctact tcgcagtgtg ggagaactag
    28621 gcgattgggg atattttcca acaccagaag cacagtgtct aggtgagcat cggttcaatt
    28681 acagcattga actacatgga ccagaagaaa aattctctac ttatttgcat gcatacgcag
    28741 cacagattcc tttttccact caacaaatca aacatcatga aggaacattg atttcaaaac
    28801 aacaatattt aacgataaaa agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt
    28861 ttagtgataa agtagtagta agaggtttca atatgtctag tcatttagaa aaattggaaa
    28921 taactaaaga caatggaaaa actgtcatat tgaatctgct tgaagaacca acgaagcaag
    28981 cggttgtccc aataatccaa ccttatgaga ttcggacaat cggattcgag gaggaaaacg
    29041 aatgtatggc ggaatagaag caggcggaac aaaatttgtg tgtgcggttt cagatggtgg
    29101 agaaatttta gaaaaattaa gtatccctac cacaacacca gaagaaacat tggatcaggt
    29161 atttgaattt tttgatcgtt atgaattgaa gggaatcggg attggctcat tcggcccgat
    29221 tggtatcgac aaaacaaaag ataattacgg atacgttttg gcaactccca aaaaagggtg
    29281 ggcacaattc gattttcttg gcacaatcaa ggaacgctac aatattcctg ctgcttggac
    29341 aacggatgtc aatgctgctg catatgggga attgaagcaa ggagcagcac aaggaaaaaa
    29401 tagttgtatt taccttaccg tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga
    29461 aattttttca gggatcgcac atcctgaaat gggacatatt tgggtgaaac gccatccaga
    29521 tgaccattat gaaggtactt gtccttatca taaagattgt ttagaaggat tagcagcagg
    29581 accgtctatt gaggcacgca cagggatcaa agggcaagat ctgccgcaag atcatcctgt
    29641 ttgggctatt caggcttatt atattgctca agcactgatc aactatacgt tgacgttagc
    29701 acctgaaaag attattttag gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat
    29761 ccgtcagcaa tttgttgaat tgatgggtgg ttatatggaa acacctcaag taagtgaata
    29821 tatcgttcgt tggggactgc cgaatgaaag tgggattatc ggtagtttgc tgttagcaga
    29881 acacgaacat caaaagtaag cagacaaatt acacaaaaag aacagagtct tatcattatc
    29941 tgctgataag actctgttct tttttatttc tattcccata atccaggtaa agctttaccg
    30001 actatttttt cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg gagcatcagt
    30061 cgttcagctg ttctattttg gtagagaggg tctccaacga tcggatgtcc cttatgagct
    30121 aaatgaactc tgatctggtg tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt
    30181 tcagaaagta ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt tttatcatct
    30241 acgacccttt ttctccgatc atggcgatct cgtccaattt tattggtgat ggtcatatct
    30301 ttggcaattt ctccccatac gatggcttga tatctccgat agatcatttt ttgttccaac
    30361 attcttccaa gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc accgcttgtt
    30421 tctttgtcca agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct
    30481 aaatgattga gtaaggtact gttttctgtc ggttcatttg gatgagtctt cattcctgca
    30541 ggcttatcca ctataatcaa atgctcatct tcatataaaa cacggatgtg cttcgcatct
    30601 cctaattgaa cttcttgata attataatct gtttcttcga ataacaatgt gatctggtca
    30661 ttgcccttta cttcatgatg gaacaaagca ggttgttgat tgatccaaac gtttttacgt
    30721 atacgcaaaa agtgtcgaac ctttctagga actaaccatt ccttttcaag caagtcgcga
    30781 attgttgttt caggatggtt cataggtaaa gtgatcgtga ttttcacaag tgtactcctt
    30841 tcagtgcaat tgcagactaa ttgtaccatt tttaaaacat atttaagaaa aaacttacat
    30901 ttttctgttc attcttatgt tactctactt gcactaacgt aaaaaaacat attgaactac
    30961 aacttaagca ggaagaaaac actctttcac tatggtaaaa taagacgatt gaatcaaata
    31021 gacaggagaa gaagcaaatg gattttcaaa gcattttaga aaagataaaa gctgctttga
    31081 tcagtttttg gcgttggatc agaccatatc tcggacaatt ccatcgatgg cgaaagcgca
    31141 tctggaaaaa atatcatgtg aacaaattga ttttgctgat tggtttgatc tgtgtcttag
    31201 tgacaagtat ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag
    31261 gattaagcca atcgacagtc atttatgata agaaaaacga agaagctggc acactgtacg
    31321 cccaaaaagg aacatatgta gaattaaacg cgatttctcc ctatatccaa gatgcagtaa
    31381 tctcgacaga agataggaat ttttacgagc atcacgggtt tgatatcaaa gggattgccc
    31441 gtgcagcagt ccgaatggtc ttgtctggcg gaacatcagg gggcggagga agtacgatca
    31501 ctcagcagct tgccaaaaat gcgtatctga cattagacca aacgtttgat cgaaaagcga
    31561 aggaattatt tttggcaatc gagatcgaaa agaagtacag caaagaagaa atcttgacca
    31621 tgtatcttaa caatgcctat tttggcaacg gggtatgggg tgtccaagac gcttccagaa
    31681 agtattttgg agtagatgca acgaatgtta cgctgtctga ggctgctact ttggcaggaa
    31741 tgctgaaagg tcctggtatt tataatccga tcgatcaccc aaaaaatgcg aatgatcgga
    31801 aaaacacagt actaagtgta atggaagaaa acggaaaaat cacaaaagaa caagaacaag
    31861 cagaaagcca gacagatatc agcgcgtatc ttaatgacac atacgataat tcagattctg
    31921 gttatcgtta tccttattat tttgatgcag tgatcgatga agcagttaac gagtatggtc
    31981 ttgatgaaga agatgtcatg aataaaggct ataaaatcta tacagcttta gatcaggatt
    32041 atcaaaagca attagaagct acttatcaaa acagtgcatt attcccagca aatgcttcag
    32101 atggtgccat ggtccaatct gcttcagtag ctttagatcc gaatacaggt ggtgtacaag
    32161 cactagtagg acgaagagga gatcatgtct ttagaggatt cagctttgct acacagatgc
    32221 gtcgttcacc aggatcaacg atcaaaccat taagcgtata tacaccagca ctggaagctg
    32281 gatacaagcc ttccagtatc ttgaaagacg aaccgcaaag ttattacgat gcgcataatt
    32341 ttgacgggac ataccaagga gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt
    32401 tgcctgctgt atggcttttg catgagatcg gtttacaaaa aggatatgat aaagcagaag
    32461 aattcggcct gcctttagca aaatctgata agtattatgg cttagccctt ggtggactgg
    32521 aaaaaggagt atctccattg atcatggcgg gtgcttacag tgcatttgcc aatgatggtc
    32581 agatgtatac gccgcatctt atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg
    32641 ataacacaaa caaaaaacca aaacaggtca tatcaaagga aacagccgat gaaatgacca
    32701 gcatgcttct tggtacattt tcaaatggaa cagcggctgc tgcgaatcct gcaggttata
    32761 cgatagcagg gaaaacagga accacagaaa ctaatttcga tgccacaaaa gcaaatgacc
    32821 aatggatgat cggttacacg ccagacgtag tgatttcgac atggatgggt tacgaagaat
    32881 ccagcaagct gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat
    32941 cagcggcaga aggcatcctt ccgtatactt caaaaacagg atttagtgta gcagatgcct
    33001 atgccacagg aggacaagtg gtagctgcag atcaagttgg aaacacagga aattctaatg
    33061 gagagtctgg aaattggcaa gataacttga atcaatatgg tgaacaagca aaagaagggc
    33121 tgaaaaactt cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag
    33181 acctctggcg gaaatatcaa ggagagtagc ccctttgaaa acgaataacc ccatgttaca
    33241 atagagagta actactaaaa gaaaagaggg cataacatgt caaacatcta cgatagcgcg
    33301 aatcaaatcg aacgtgagat ccgtgaatta ccagaattca aagcgttgaa agaagcgtac
    33361 gagaaagtac aagcaaacga agaagcacac aaactgttca aagacttcca agcgatgcaa
    33421 atcgaattac aagaaaaaca aatgagtgga caagagttca gcgacgaaga tgcaatgaaa
    33481 gcacaagaaa tggctgtgaa aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa
    33541 gagcaaggct tcagtacaat catcaatgac ttgaaccgta tcatcatgac accagttcgc
    33601 gacctttaca gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa gagacgttcg
    33661 gccttgagcg atgacaaaat tgcttcttat tgccgatagg ctgtctcttg aacaccattt
    33721 accaagaggt cgtgagccta ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca
    33781 gcttctcatg tttgatgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
    33841 ttattcagaa ataggagcta cgacaaaagt cttgtcatag ctcctatttt ttgtcattca
    33901 tttttcaagc ggtagaaata gcctttttga gctaacagtt tttcgtaagt acctatttca
    33961 acgatttttc cttcatcgat cacaacgatt ttatctgcct ctttgatcgt tgaaagccga
    34021 tgagccacaa taaaagtagt gggtgtttct gtcaggtaat tcagggcttg ctggattttt
    34081 ttctctgact ggttatctaa agctgaggtt gcttcatcta gcaaaatgat ttttggctga
    34141 cgtatcaacg ctcgtgcaat ggagatacat tgcttttggc caccagacag cttgttgcca
    34201 tgttcgccta ctaaggtatc cagaccatca ggaagctggt caatcagtga agaaagttgc
    34261 gcagcttcga tgacttcatc taaacgttct tttgatacgt ttttcaagcc ataagtgatg
    34321 ttctctttga tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc taaataattt
    34381 cgcacacttc gcatatccag ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa
    34441 gtcggctgga taaatccaat caataagtta aggagtgtag attttccaga gccagatggc
    34501 ccaacaaaag caattgtttc tccagctttc acctctagag aaaaatgatc gataatcggt
    34561 ttgagtgagt ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt
    34621 tggatttttt tcttaccgtg ataagtttct acatcgccag caatcaacag ttcgccaata
    34681 gaagtgatag attccatgcc ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg
    34741 gaattgacga tcgtcgtgaa atacgtctga tacatcacga catcgccggg ttggatgcgc
    34801 ccttgtaaag ctaatatccc agtgaaaatc aaacaaatca gctgaaatat ctgaaaacta
    34861 gcccagctga ctgatccgaa aacagattgc aggatatcta agtggaatcc tttttcatag
    34921 atttgagtaa aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc atgagctcga
    34981 gcaatcggtg ctaattcgat cacttcgatc acttttgcag aagtctcttc catttcttgc
    35041 ctgaagtctg agttggactg tctgatcctt ttacggaaaa agagagtaac gaatgcggcg
    35101 agaggaacag tcaataagaa aaacatgaaa acaacaggac ttttataaag cgtgatacct
    35161 aaagctacga ataaattgac agcgatattt aatccgctga caaatacttg agaggacaac
    35221 gtttggatag cttctacatc acggatgatc tttgactgga ttcgtccaga ttgcaattct
    35281 ttttgataag gaatagatag ttcttggatt ttcttgataa gggtcgctct tagtccagct
    35341 tctacttgac ggatcgaagt gctatggtat tttacatgaa aataattcgt caatatattt
    35401 tgaccaatca gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa ctgagaagaa
    35461 tctttcgatg aagctgcatt gattacattg gcaatagcga tcggcattaa ccaagatgga
    35521 agatgtttga taataaaaaa gagtcctgac atggcaaaac gccaataatt tccctgataa
    35581 agatgcagca atgtctgaaa agcatttgca tgatattttt gataactttt ctgtattcgt
    35641 tcctccataa tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt ctattgcttc
    35701 aggaacataa atctcaggag tagcttcaag agtggcgtac attagaggac gctcatattt
    35761 gatataatgc agtaccgggc ggaaatctaa acagcccttg ccaacaggta ccatgcggat
    35821 ctttccattt tccatgatat agtctttgag atgaagcgct gtaatataac catgtaaatt
    35881 gtccaaggca cctcggatca ctttttcctg atcagcatga ttttctttat gcatcaaatt
    35941 cgcacaatct aaaatgattt ttaaatttgg tgaagctact tcatcgatta aacgtttagc
    36001 taactgatag ttatagattg gatgattgat ccctgcttca acagcaacag ttacgccaaa
    36061 attttctgca tgagcgacta attcaatgat agaatttctg gcgatctgat aagcttcttc
    36121 agtaaaattt tcttttgtgt aaccgacaga gacactaccg gtttccgtag caatcatctt
    36181 ggcttgatag gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg cttgtgcacg
    36241 aacttcttga tttttgctgg ataggttgat atagcatcct aaaatggaaa gttcgatatt
    36301 gtttttttgg aagtattcac caaaataggc agcactccca agtgtcatgt cagttaattt
    36361 aagttgttca ggaaatgctt tttgtggtgc aaattggata tgggacaagt gaagctgtga
    36421 tactttttga acgatttctt cacgatttcg aacatctaaa tcgtgggcac ggatgcctaa
    36481 atttaatgga aacatctcaa actccttttt gtaaataatt agcaagaggc agacgggatt
    36541 cttttatcaa ttcaacaatt attcgggcaa ctttatcagc gccttctgga ctaaagtggg
    36601 tattgtcttt tagtccttct gggtaatttt tacaagtatc aggcataaga tgcaggtaga
    36661 atttttttgt ctcttctttt gagaaggtct ggaacaattc ttgtgatctt gagaacacat
    36721 ctaaaagagg aatatggtgt ttctcagcaa atgcacgcat cgcttttgga tagtctccaa
    36781 gtatatctgg attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca gatgtcagta
    36841 agaccatttg gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga ttttttaaat
    36901 agctaccgaa gggaagcgta cctctttctt cttggatttt ctggtcgtta tgtccaaatt
    36961 gtacaaataa gaaatcattc ggctgaaact gtgaagctag acgggctaac cttccttcct
    37021 ggataaatga tttggtgctg cgtccattct ttgcttggtt attgatctgg atctcgggtg
    37081 aaaaataagc agagattttt tctccccaac ctgtctcggg acgcttgttt tcatcttttt
    37141 ctgccgcagt agaatcaccg gcaatatgga tagtaggcat gttatctctt ctttcataaa
    37201 ggtttatagg caatgaagca gaacagcaag agaagaagac ggagcaa
//
LOCUS       DAZBTH010000018        34466 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000018 DAZBTH010000000
VERSION     DAZBTH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.018"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..560
                     /locus_tag="ACHGNW_000873"
     CDS             168..560
                     /locus_tag="ACHGNW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6925208.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            588..1928
                     /gene="glnA"
                     /locus_tag="ACHGNW_000874"
     CDS             588..1928
                     /gene="glnA"
                     /locus_tag="ACHGNW_000874"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN6925209.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2654..2899
                     /locus_tag="ACHGNW_000875"
     CDS             2654..2899
                     /locus_tag="ACHGNW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925210.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3121..4731
                     /locus_tag="ACHGNW_000876"
     CDS             3121..4731
                     /locus_tag="ACHGNW_000876"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN6925211.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4708..4920)
                     /locus_tag="ACHGNW_000877"
     CDS             complement(4708..4920)
                     /locus_tag="ACHGNW_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925212.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5023..5892
                     /locus_tag="ACHGNW_000878"
     CDS             5023..5892
                     /locus_tag="ACHGNW_000878"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN6925213.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6057..7328
                     /locus_tag="ACHGNW_000879"
     CDS             6057..7328
                     /locus_tag="ACHGNW_000879"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6925214.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7325..8620
                     /gene="rho"
                     /locus_tag="ACHGNW_000880"
     CDS             7325..8620
                     /gene="rho"
                     /locus_tag="ACHGNW_000880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN6925215.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8719..8982
                     /locus_tag="ACHGNW_000881"
     CDS             8719..8982
                     /locus_tag="ACHGNW_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN6925216.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9427..9876
                     /locus_tag="ACHGNW_000882"
     CDS             9427..9876
                     /locus_tag="ACHGNW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6925217.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9933..11351
                     /locus_tag="ACHGNW_000883"
     CDS             9933..11351
                     /locus_tag="ACHGNW_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6925218.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11351..12211
                     /locus_tag="ACHGNW_000884"
     CDS             11351..12211
                     /locus_tag="ACHGNW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6925219.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12385..13158
                     /locus_tag="ACHGNW_000885"
     CDS             12385..13158
                     /locus_tag="ACHGNW_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6925220.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13305..13670
                     /locus_tag="ACHGNW_000886"
     CDS             13305..13670
                     /locus_tag="ACHGNW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN6925221.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13818..15026)
                     /locus_tag="ACHGNW_000887"
     CDS             complement(13818..15026)
                     /locus_tag="ACHGNW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925222.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15045..15323)
                     /locus_tag="ACHGNW_000888"
     CDS             complement(15045..15323)
                     /locus_tag="ACHGNW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925223.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15516..16172
                     /locus_tag="ACHGNW_000889"
     CDS             15516..16172
                     /locus_tag="ACHGNW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6925224.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16614..17411)
                     /locus_tag="ACHGNW_000890"
     CDS             complement(16614..17411)
                     /locus_tag="ACHGNW_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN6925225.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17408..18592)
                     /locus_tag="ACHGNW_000891"
     CDS             complement(17408..18592)
                     /locus_tag="ACHGNW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN6925226.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18617..19864)
                     /locus_tag="ACHGNW_000892"
     CDS             complement(18617..19864)
                     /locus_tag="ACHGNW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6925227.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20079..21824
                     /gene="dnaX"
                     /locus_tag="ACHGNW_000893"
     CDS             20079..21824
                     /gene="dnaX"
                     /locus_tag="ACHGNW_000893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN6925228.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21844..22158
                     /locus_tag="ACHGNW_000894"
     CDS             21844..22158
                     /locus_tag="ACHGNW_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN6925229.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22183..22779
                     /gene="recR"
                     /locus_tag="ACHGNW_000895"
     CDS             22183..22779
                     /gene="recR"
                     /locus_tag="ACHGNW_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN6925230.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22843..23487
                     /gene="tmk"
                     /locus_tag="ACHGNW_000896"
     CDS             22843..23487
                     /gene="tmk"
                     /locus_tag="ACHGNW_000896"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN6925231.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23502..23831
                     /locus_tag="ACHGNW_000897"
     CDS             23502..23831
                     /locus_tag="ACHGNW_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN6925232.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23845..24783
                     /gene="holB"
                     /locus_tag="ACHGNW_000898"
     CDS             23845..24783
                     /gene="holB"
                     /locus_tag="ACHGNW_000898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN6925233.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24819..25643
                     /locus_tag="ACHGNW_000899"
     CDS             24819..25643
                     /locus_tag="ACHGNW_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN6925234.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25636..25983
                     /locus_tag="ACHGNW_000900"
     CDS             25636..25983
                     /locus_tag="ACHGNW_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN6925235.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26052..26924
                     /gene="rsmI"
                     /locus_tag="ACHGNW_000901"
     CDS             26052..26924
                     /gene="rsmI"
                     /locus_tag="ACHGNW_000901"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN6925236.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27033..28154
                     /gene="dinB"
                     /locus_tag="ACHGNW_000902"
     CDS             27033..28154
                     /gene="dinB"
                     /locus_tag="ACHGNW_000902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN6925237.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28208..28810)
                     /gene="nrdG"
                     /locus_tag="ACHGNW_000903"
     CDS             complement(28208..28810)
                     /gene="nrdG"
                     /locus_tag="ACHGNW_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN6925238.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29125..31314)
                     /gene="nrdD"
                     /locus_tag="ACHGNW_000904"
     CDS             complement(29125..31314)
                     /gene="nrdD"
                     /locus_tag="ACHGNW_000904"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN6925239.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31640..32407
                     /locus_tag="ACHGNW_000905"
     CDS             31640..32407
                     /locus_tag="ACHGNW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925240.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32397..34439
                     /locus_tag="ACHGNW_000906"
     CDS             32397..34439
                     /locus_tag="ACHGNW_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925241.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11066 a         6117 c         7245 g        10038 t
ORIGIN      
        1 aaggtatctg gtacgaaaat caactagata ccttttttgt gttcttattt ttttgttatt
       61 ttataaaaaa tagagatctc atgttagaaa acctaacata tgcagttgac aatatttttt
      121 ttaattggta cactttagcc aacttataaa acgaaagggg acgtctgatg ggagaaaagg
      181 aattgcgccg atctatgtcc gtatttccaa ttggtacagt tatgaagctg actgatttgt
      241 ctgctcggca aattcgctat tatgaagaac aagatttagt tcatccggaa cgaagcgaag
      301 gaaaccgacg tatgtattcg ctgaatgata ttgatatgtt gcttgagatt aaagactatc
      361 tttcagaggg attgaatatg gctgggatca aacgtgtata cgaaatggca caggcgaaaa
      421 aacaagaagc aaaaaacaag aagccgttga cagatcatga cgttcgacaa atttttcgtg
      481 atgagatttt ggcgcaaggt gggttaagca aaacaggtca ttatcagtcg caaggattgc
      541 aaagacaatt ctttgactga ataaattaaa aaaggaagag ggataccatg gtaaagaaac
      601 agataacagg tgaagaaatc aaacgaatca ttgaggaaga aaacgtacga tttttacgat
      661 tgatgttcac agacatctta ggtacgatca aaaatgtaga agttccagtg agccagatcg
      721 acaaagtatt agaaaataaa atgatgtttg acggttcttc gattgaagga tttgttcgaa
      781 ttgaagaaag cgatatgtat ttgtatccag atctctccac ttggatgatc ttcccgtggg
      841 aaagtgctca tggaaaagtt gctcgtttaa tttgtgatat ctataatccg gatgggacac
      901 catttgccgg cgatcctcgc gggaatttga aacgagcatt gaaagatatg cgcgaccttg
      961 gttttacctc tttcaattta ggacctgaac cagaattctt cttatttaaa ttagacgaag
     1021 acggtgggat cacaacgacc ttgaatgata aaggtggtta ttttgatttt gcaccaacag
     1081 atttaggtga aaattgccgc cgtgacatcg tgttggaatt agaaagtcta ggctttgaag
     1141 tggaagcttc acatcatgaa gtagcgccag gacagcatga aattgacttt aaatatgcag
     1201 atgtggtaga tgcttgtgat aatatccaaa ccttcaagct cgttgtcaaa acgattgcac
     1261 gcaagcatgg acttcacgcg actttcatgc cgaaaccgtt atttgggatc aatggttctg
     1321 gtatgcattg caatatgtct ttgttcaatg atgaaggaaa tgttttctat gataaagacg
     1381 gtgaattgga actaagtgaa acagcttatc atttcttagg tggtttgctg aaacatgctc
     1441 gtgcctacac agccgtttgc aatccaacag tcaattctta taaacgtctc gttccaggtt
     1501 atgaagcgcc tgtttatgtc gcatggagtg gccgtaatcg ttcgccatta gtacgtgtac
     1561 cagaatctcg cggtttatcc acacgtttgg aattgcggtc agttgatcca tctgcaaacc
     1621 catacttggc aatggcagta ttgttgcaag caggactaga cggtatccgt aatcagatca
     1681 caccgccagc agctgttgac cgtaacattt atgtaatgga tgaggaagaa agagaagctg
     1741 cacatatcca ggacttacct tcaactatcc ataatgctat caaagaattg cgtaaggata
     1801 atgtaatgat tgacgcatta ggacaacata tcttctcaaa ttttgttgaa gcaaaacgtc
     1861 tcgaatgggc ggcattcaga caaactgttt ccgagtggga aagagaacaa tatctagaac
     1921 tttattaaaa caggaatacc gtgaattaga ggctgtgaaa tcagcgtttt gacctgagta
     1981 ttagagaaac taacggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     2041 gggtcagctg attgaacacc gtttagtgga gaggttgtga aaaagctgcc ctgcaacaag
     2101 taataagaat agccaagcaa aaacagcttt tcttgttttt gaattggctg aacttattac
     2161 cgcagatgca agcttttgaa caccgtttac caagaggttt tgaaaaagac gtttagtttc
     2221 gagtattaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa tttggcttaa
     2281 taccgagaaa ctggcttttg aataccgttt atccagaggt tgtgagccta ccatttaact
     2341 ccgaatcaca agaaataatt tcaaaaacag cttctcatgt ttgatggata ttgtgacttg
     2401 tgaccgagga gttggcagga gaacaccgtt tatttagaaa taaggaggca cgacttttgt
     2461 cgcatctcct tattttttac ttatgtgatt cgttaccttc aatgaatccc tacaaggcac
     2521 ttttgttgaa aaaaaggtca tctttgaaaa aattagtgga attgttttgt ttttgatatt
     2581 atttaataga aaagatatgc caaaaatccg agaactgtgg actacgtttt ttacgtagag
     2641 gaggtaaata agggtgaaag aaaaagattg tttctacgat aagctatata aactgttgct
     2701 gcaagtagga aaaagtagtt ctttgttagg tgggatatta tctatcataa tgtttgtttt
     2761 atttttttat ctattatttc acttaaaatt tttcagtaac gattttctga tttgcttagg
     2821 tttaacgtta gctatgttta tatcaagctt attatgtggg ttaaattttt tttgcactca
     2881 taaaggactt aagaaataat ttctcaaagg atatcgaaaa agaaaaaatt gtaagcggat
     2941 agtattcatt tgagtacttg actttccctt agtcagcttg tattatacac cttgggcacc
     3001 cttttagagg gtcagaagtt tacagaaatt tcggctccct attcttattg aagaataggg
     3061 agctttgttt attgtctgag acttttcctg cacataaatt aaaaaggagt ttttgaactt
     3121 atgacaaaat atatttttgt gaccggcggt gtcgtttcat caatcggaaa agggattgtt
     3181 gcagcttctt taggacgctt attgaaaaac cgcggcttaa aagtaactat ccaaaaattt
     3241 gatccataca tcaacgtgga tccggggacg atgagcccat atcagcacgg agaagtattc
     3301 gtaacagatg atggtgctga aacagatttg gatctgggcc actacgaacg ttttattgac
     3361 atcaatttga acaaatactc caacgtgacg acaggaaaaa tctattctga agttttgcgt
     3421 aaagaacgta agggcgaata cttgggagca actgttcaag ttattcctca tattacaaat
     3481 gagatcaaag aaaaaatcat gcgagcagct aaaatgactg attcagatgt gatcattaca
     3541 gaagtagggg gaacagtagg ggatatagaa tctttgccat tccttgaagc acttcgtcaa
     3601 atgaaagcag atgtcggtac agataatgtt atgtatatcc atacaacatt gattccttac
     3661 ttgaaagctg ctggagaaat gaaaactaaa ccaacgcagc acagtgtaaa agagctacgt
     3721 ggtttaggta tccaaccgaa tattttggtg gtacgtacag agcaacctgt ttctcaaagc
     3781 gtgaaaaaca aattggcaca attttgtgat gtcgaaccag aagcagttat cgagtcgcca
     3841 gatgtagata cactttactc catcccatta gttttacaag cacaaggaat ggatcaaatt
     3901 gtttgcgacc atttaaaact agatacacca gaagcagaca tgacagaatg gaaagaatta
     3961 gaagaacgtg tcttgaactt gaagaaaaaa gtacggattg cgcttgtcgg taaatatgtc
     4021 gagttaccag atgcatatat ctcagtagtt gaagcattga aacattctgg tttcgcctat
     4081 aattcagaca tcgagatcga ttggatcaaa gcacaagaat taacgagaga aaatgtagaa
     4141 gaacgtttga aagacgcaga tggaatcttg gttcctggtg gatttggtga tcgcggagta
     4201 gaagggaaaa tcgaagcgat tcgttatgct cgcgaaaatg acgttccgtt tttaggtatt
     4261 tgtcttggta tgcagatggc ttgtgtcgaa tttgcacgta acgtcgttgg attagaagat
     4321 gcgtcatcag ctgaaacgaa tccagataca gcaaacaata ttattgattt aatggctgat
     4381 caggaaaaca tcgagaattt gggtggaact ttacgccttg gattataccc atgtaaaatc
     4441 aaaaaaggaa cgaagacggc tgaagcatat gatggagccg atgttgtgca agaacgtcac
     4501 cgtcaccgct atgagttcaa taacaaatat cgtcaattgt ttgaagaaca agggctagtc
     4561 ttttcaggtg tctcaccaga taaccgttta gtagaaatcg tagagctgcc agagaaaaaa
     4621 ttctttgtgg gatgccaatt ccacccagaa ttgatttctc gtccaaatcg cccgcaaaaa
     4681 ttaatcaaag catttgtcgg agcatcatta gcagatcacg aaacaaaata atttttgaac
     4741 ttgatagaaa gagaaaagta gcaagagaag gggataagca attttcttgt cacttttctc
     4801 tttttctgat ttgttattat ttgtaggaaa aaagatagaa agcaaaagac ttttttcgat
     4861 taaagcgcta ttggatattt tattttttgc aaaaaacttc ttgaaaattt tcattttcat
     4921 tgattttagt agaaaactac cgaaatgttt ggtaaaatag ggtcgttggt taagaattgt
     4981 cttaacaaat ttagttttac aaaacttagg aggaatttta ttatgccagt agtatcagga
     5041 gctgaatttt taaaagctgc tcgtaaaggc ggttacgctg tcggcggatt caacacaaac
     5101 aacttggaat ggactcaagc aatccttgaa gcggctgaag caaaacaagc accagtactt
     5161 atccaaacat caatgggtgc agctaaatac atgggtggtt acaaaatctg taaagattta
     5221 atcactgatt tagttgaagc aatgaacatc actgttccag ttgctatcca cttagaccat
     5281 ggtgattacg aagctgcttt ggaatgtatc gaattaggct atacttcagt aatgttcgat
     5341 ggttcacatc taccatttga agaaaacttg aaattagcta aagacgttgt tgaaaaagca
     5401 catgcaaaag gtatctcagt tgaatgtgaa gttggttcaa tcggcggaga agaagacgga
     5461 atcatcggta acggcgaatt agctgatatt gaagaatgca aacaaatggt tgctacaggt
     5521 atcgactact tagcttgcgg tatcggtaac atccatggtc aatatccaga aaactggaaa
     5581 ggattagctt ttgaccactt acaagcaatt gctgatgctg taggtccaga catgccttta
     5641 gtattacatg gcggatcagg tattcctcaa gatcaaatcg aaaaagctat ctcaatgggt
     5701 atctcaaaag taaatgttaa cactgaattc caattatcat ttgctgcagc tactcgtaaa
     5761 tatatcgaag ctggtaaaga taaagaaggt aaaggattcg accctcgtaa attattagct
     5821 ccaggtaaag cagcaatcgt tgaagatgct aaatctcata tcgactggtt cggttcagct
     5881 aacaaagcgt aagcatccac ataacagata cgatatatag gaatggagaa cgacttttga
     5941 agtcgctctc cattcttttt tacttaaaac ggcagaccat gctcgtttct tttttttatc
     6001 tatgataaaa tacaagcata tccatgaaaa aagattaata aacaaggtgg aaacagatga
     6061 aaaaaatcgt aatcaaagga aaccgacctt tagctggtga agtaacgatc agcggtgcaa
     6121 aaaatagtgc agtcgcattg attccagctg caatcttagc tgattcccca gtgatcttgg
     6181 aaggtgtgcc ggacatacaa gatgttcatt cattaattga aattttagag atcatgggcg
     6241 caactgtcca tttttccaac aacatactag aaatcgatcc tcgagaagtc gtatcgattc
     6301 cgatgccgca cggaaaaatc aatagtttgc gtgcttctta ttattttatg ggaacactgc
     6361 ttggtaaatt tggcgaagcg gtagtaggcc tgccaggtgg ctgttattta ggccctagac
     6421 cgattgacct acacgtcaaa ggatttgaag ctctaggtgc tgaagtgaca aatgaacatg
     6481 gtgccatgta tttacggaca gaaaataaag gattgcgtgg caatcggatt ttcatggacg
     6541 tggtttcagt cggagcaacg atcaatgtga tgttagctgc tgtaaaagca aaaggacaga
     6601 cagtaattga aaacgctgca cgagaaccgg aaatcataga tgtggcaact ttactgaata
     6661 atatgggggc aaaagttcga ggagcgggta cagatgtgat ccgtatcgaa ggagtcgaaa
     6721 cattacatgg ctgtcggcat ttcatgattc ctgaccgcat cgaagcagga acctatctag
     6781 cattagcagc tgcagtagga aatggtatca aagtgaaaaa tgtgatcttt gaacatttgg
     6841 aaagctttat agcaaaattg caggaaattg gtgtccatat gaaaatcagc gaagatgaaa
     6901 tcgaagttta cccctcgaaa gaattaaaac ccgcaaacat catgacctat ccttatccag
     6961 gatttgccac agatctgcag cagccactta ctgcgttact attgatgact acaggcactt
     7021 cagaaatcat cgatacgatc tatgcgaaac gtgtgaacca tgtgccggaa ctagcacgaa
     7081 tgggcgctga tattaccgtg gaaggaaata tgatcatcgt gaatggtcca aataaacttc
     7141 acggcacaga agtcgtggct tctgatcttc gggcaggtgc ttgtttagtg attgctggac
     7201 tattggcaga agggacaacg acgatttaca atgtagatta tatcttacgc ggttatgatc
     7261 atattatcga gaaactaaca gccttagggg catcaattga aatgatagag gaagtgggag
     7321 ctgaatgagc gattatttga caatggcaga actagagaac aaaacgttaa aagaagttta
     7381 tagttatgcc aaagaattta aaattcctta ttacagtaag atgaataaaa aggaattatc
     7441 tctagctgtg attcgtgcac aagcagagaa acaaggattt tattatatgg aaggaatatt
     7501 ggatatcgtt ggtcaagatg gttacggttt tttacggcca atcaattatg gaccaagtgc
     7561 agaagatatc tatatttctg cctcacaaat cagacgattc agtttgagaa atggggacaa
     7621 agtggcagga aaagcccgtc cacctaaaga atcggaacgt tattatggtt tgatgcacgt
     7681 ggaaagcgtt aatggaaaag atccggaaga agcaaaagaa cgtccgcatt ttccagcctt
     7741 aacacctctt tatccgcaaa gacagcttca actggaaaca acaccgcaaa gactatctac
     7801 ccgaatgatc gatatctttt ctccaatcgg ttttggtcag cgtggattga tcgtcgcacc
     7861 gccaaaagca gggaaaactt ctgtattaaa agaaatcgca aatggtatca ccgaaaacta
     7921 tccggaagta gaattaatcg ttctactgat tgatgaacga ccggaagaag taacggatct
     7981 agaacgaagc gtgaaaggcg acgtggtatc ttctacattt gatctgcagc cgcaaaacca
     8041 tactcgagtt tctgaattag tattggaacg cgcaatgcgt ttagtcgaag ataaacgaga
     8101 tgtcgttatc ttgatggata gtattactag acttgctcgg gcctataact tagtcgttcc
     8161 accaagcgga cgtacattga gtggcgggat tgacccagcc gcattgtata aacctaagaa
     8221 atttttcggg gcagcaagaa acatcgaaga aggtggcagt ttgacgattt tagcgaccgc
     8281 tttagtcgat acaggaagcc gtatggatga cgtgatctat gaagaattca aaggaactgg
     8341 aaatctagaa ttacaacttt cacgtgaatt atctgaacgt cggatcttcc cagccatcga
     8401 tatcaaaaaa tcaggtaccc gaaaagaaga attattgatg ccaagtgaag aattagaaga
     8461 aatttggaaa cttcgaaaaa atatgactgg agattcatta gaatatacgg aacaactgat
     8521 ccgctttttg cgtaaaacaa aaaacaatca gcaatttttc aaagaatttc aagatgtctc
     8581 ctttggtaaa aaaaatccga ccagaaatct gaaaagataa ttgcaacctt gactgaaaca
     8641 tgttaagatg ttaatgttgt aaatcgacaa atcaactctg gttcagcaaa tggagcaggg
     8701 caaaggagcg aaaaaatcat gaaagaaaac atccatccag attaccaccc agtagtattc
     8761 atggattcaa caactggttt caaattcttg tcaggttcaa caaaaacttc acaagaaaca
     8821 gttgaatggg aagacggtaa tacataccca gtcatccgtg tcgaagtaac ttctgactca
     8881 catccattct acactggacg tcaaaaattc acacaagcag acggacgtgt ggaccgtttc
     8941 aacaaaaaat acggtctcaa agacgcaaac gctgcggaat aataaaaacg ctgataactc
     9001 aacgtttagc agactatctg atagggatag tctgtttttt tatgtttttt gccaatttaa
     9061 aacttcgttt gatgctgaaa aagtcgtagg atcataatgc acaaaacaat gaaaaactag
     9121 tatatagttt taattatcca cttgataaaa acaccttata ttaacgaaat gaagaggggt
     9181 tatttcgaag taaaaaacca tttacctaaa attagaagct aagctttcct ttcaaggaag
     9241 gctttttgtt ttgttataga cttgcgattg tttgtgagct agatcgttca ttttaatgac
     9301 ttatacacgc ggatgatggg tctttttcct ctgttcattc ataatattga acggtacagc
     9361 tagcgatgct atactgacct cagttccaag aaaacgttaa cattttttgt gggggagagg
     9421 ttttttatga ggtttgatca attgatcgca gaagaactga ttgttttcga tccatcgata
     9481 gattcaaaag gatcattatt tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta
     9541 agaaatgttc aaaaagtcgt taaggcacta ttcaaacgag aggaggagat ctccacagga
     9601 atagaagatg gatttgggat tccccatgca aaaagcaaat atgtgacaaa accagcactg
     9661 tgtttttttc atacagccaa attaacggac tatagcggga tagatgaaat gccaattgaa
     9721 tatatttttg ccatcgtagt tcctgaaaaa tcagcagata tgcatctaga gattctcagc
     9781 acactctcca gacgattgat gaatgcagat tttagaaagg gaataaaaga agccactacg
     9841 ccagcagata tcatcagaca gattagtcaa gaatgaaaat agtagagtct ttgtccgagt
     9901 atcgatagtg aagaaaaagg aggaaaataa tcatgaaaat cgttggtgta actgcttgcc
     9961 cgactggaat tgctcatact tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc
    10021 taaaccatca agcaaaattc gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac
    10081 aagaaatcaa agaggcagat gcaatcattc ttgcagtaga taaagaaatc gaattggacc
    10141 gatttgccgg gaaaaaagta aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag
    10201 tagtaataga cgaagcattg agagatattg gcgtattcgt tgtgtctaat gaaaaagaac
    10261 cagctgcaaa tgagaaaaag ccgacaatct ataatcattt catgaatggt gtgaattaca
    10321 tgcttccatt tgtgatcgca ggcggaatca tcatcgcact gagttttgcc tttgggatta
    10381 cagcggctga tcccaactca gcagactata atgtgttagc agctgcattt tcaaggatcg
    10441 gaggagatac agcgtttgcc atgatggttc ctgcgttagg cgcggggatt gctacatcga
    10501 ttgctggaaa agcaggtttt gccccaggaa ttgttgccgg attactggcc tctacaggtg
    10561 gttcaggttt tcttggcgga atgattggtg gcgtgctagc agggtatatc tgtgattttc
    10621 tggcaaataa aattagcgta aaaaaagaat tttctgctat ttatcagctg attgtcgtcc
    10681 cctttttatc gatcttaatt attgggcttt tgatggtatt tgtgattgaa caaccgatca
    10741 catgggtgtt ggatgctttg acaaattggc tgaatagctt gggaaataca tccgggctat
    10801 tgttcggatt acttatcggg atcatgatgg cagctgatat gggtgggccg atcaataaat
    10861 cgacttcgac tttctcgatc ggtttgatgt cagctggagt aaatgcaccg attgctgctt
    10921 gtatggctgc aggaatgaca cctcccctag gtattgcttt agccacttta ttattcaaac
    10981 ataaatttac caaagaagaa cggacttcag gtcaatcatg ctggatttta ggcgcttcgt
    11041 atattacaga aggtgctata ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt
    11101 tgatgacagg ttcagcagtc gctgcaggga tttccatggc agtaggagtt acttcaatgg
    11161 cacctcatgg agggatttgg gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt
    11221 atgtcttggc aattgcatca ggtacagtca ctacagcctt tatgcttgga ctattgaaaa
    11281 aaacagtgaa agagaaaaaa caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg
    11341 tggaagcgta atgttatata caatgaaaga gttattgaaa gtggctaaag agaatcaatt
    11401 tgcagtacca gccttcaata tttgcagcta tgatatgatg aaagcaatca tcgaagaagc
    11461 agagagatta gaagcaccag taatcttaga aattcatcct gatgaaatcg cctatttaag
    11521 tgatgagttt gttgcggcag taagagccta tgcttataca agtaaactgc cgatcgttat
    11581 ccacttggat catggcggta cgatagaaga tgtcttacgg gcgattcgaa acggctattc
    11641 ttccgtgatg atcgatgcgt ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa
    11701 agtggttgcg ttagctcatg cggtaaatgt ctcagtggag gctgagcttg gaacgattgg
    11761 gaataatggt tctgctgaag gaggtgctga aacaatcatt tatacagatc ctgatcaagc
    11821 gaagattttt gtgaaagaaa caggaatcga tacacttgct gtagcaattg gaacagcgca
    11881 tggcttgtat ccgaaaaaca gaactccaaa attaaatatc gacttactga aagaattgaa
    11941 ccaacaaatc gatcttccat ttgttttaca tggcggttca ggtaatccgg acgcagaagt
    12001 cagcgaagca gtaaaatacg gagttgccaa agtgaatttg agctcagatc ttaaaagcgt
    12061 ctttttcaat gctttgagag aaatattgag tgaaaatcca tcgatgtacg agccgaatgc
    12121 aatttatcca gcagctaata aaaaggtaca agaagtggtc aaacataaaa tggaaatttt
    12181 gaacacagta ggcaaggcta gtttatacta aaaataaaaa tgtgtggtca agagggtatg
    12241 acaatagtct tgtcataccc tcttaaccac acacgatgtt cagaagtagt tttttcgaaa
    12301 aatttcttct tatttctcga agccaatcac ttctgtcaca acctcttttt tcctgttact
    12361 atataaagta aatggggtga ttttatgttg aatgaagtgg agaatagaca gcaggagatt
    12421 gtgaattatc taaaaaacaa ccaatttgca agagtcaccg atttgatcga gttagtaaat
    12481 tatagcgaag ccacgattaa acgtgacctt gtttttctgg aaaaaagtgg attgatccgt
    12541 agagttcgcg gtggcgccat gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg
    12601 aagatcacca aattagaaga agaaacagaa aaacgtaaga ttgcagatac agcttcttct
    12661 ttgattcgag atgatatggt cttattcttg gattccagta caacctcttt acacttagtc
    12721 agaaacctag caagatttga tggtttgcag atcattacga atggcatcgt cacagctgct
    12781 atgttgagtg aatatacaac ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa
    12841 cgagcaacaa tcaacggttc aaaagcctat aacgatgcat tgtcatatta tgcggattta
    12901 gcgataatga gttgtcgcgg gctagatttc agccaaggag taacagaaac tcatgaagga
    12961 gaggcaatgg tcaaaaaagc ttttagaaaa caatcagacc ggcttgccgt tttagttaca
    13021 cagcaaaaaa ttgcacataa attcatgcat caagccattg cttttcatga aatcgattat
    13081 ctggtgacaa gtaaagaact gacaaaagaa cagtatgcga aagtaaagga acaccatatc
    13141 gaatgtctgt acggataaat tattacgtga atgttctaag tggcttcctc atttttagat
    13201 aggaccttat ctttgctcgc ttctctcagc cgatggtata ataatgaaaa ggttatcttg
    13261 ttagataatc ttactttttt atccaggaga aaggagtgca gaaaatgaaa catgtgaagg
    13321 cgttggtagt gaaggcaatc atgatctggg cgattctgtg ggtcgtgctg acaggtttat
    13381 acggtgttag ctttatggat tcaacgattg ttggggtaat tattgtagtc atgatctatg
    13441 tgctagggga tctgttgatc ttacgtaaag tagggaacat cgcagcgacg atcgcagatg
    13501 ctggttcagc tgcagttatc ttgtggctct atctatattt tatgaatgat acagtcgata
    13561 tttggatgaa agtattgatt cctgctctat tgatcggtgt ggcagaatgg ttcttccaca
    13621 agtggttgct aagtcaaggc atcgtaccag atgaacggaa aatggaataa atcaacagtt
    13681 aaactattct tatcgataaa tgaaagcttt aaaaataaat agatgtttaa ttaagaataa
    13741 tcataaacta tttttactca aaaaaccgct cagattattt tcagatctga acggtttttt
    13801 ttatctattc atcaagatca aatttcattt ttcaatgatt caatcaattc gccctctgct
    13861 tcatgactat gttttcggcc attaacaaga tagagtgctg tacatatcaa tggaatgact
    13921 ccggcaatca catacaatcc tctaaatgaa gtaagagatt ttagcatccc catcaaataa
    13981 ggaccaacgc ctaagcctag gtctaaacca ataaagtagg tagataaagc aatactgatt
    14041 cgatgttcgc ggacgatttt taaacaaaca gcctgtccat ttgacataaa cgtaccataa
    14101 cctaggccga ctaatgctcc agagataagc aatgtcacac tattcgtcgt tacactcagt
    14161 aaaaataatc ctgctgttaa gaataaatag ctaggataca tcacgtattg ttctcctttg
    14221 acatcgaaaa tccttccagt caatggacgt gtaaatgtga tgaccaaagc atatacgacg
    14281 aagaaaaatg acccagcagc aactaagtgg attacttttg tataagaaga taaaaatgat
    14341 aaaacacttg aataagaaag acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc
    14401 ttttcaataa aactattgat attccatgag cgaagagata ttttttgttc ttcagataat
    14461 ggaatatttt ttacaggaaa gacaaggcag gcaatcgtaa caaagaagac cagtacaatt
    14521 gaaaaccaga taatgaaacg aaaatcagtc atattcagca aaatcattcc taaaaatggc
    14581 ccaacagcgg cagctaaact agtacttaat ccataataat tgatgccttc ccctttttta
    14641 gatgcaggga tatatgcagt aacgatagca tttgtagctg tagaaactgt accgtagccg
    14701 aatccattca agaatcgaac taaatacaat gccccgatag ttggcgtata aagatatgct
    14761 aatgttgaaa ggagatagaa gatcgcgccg cctcttaagg taaacttccg accaaatagt
    14821 tctaactgtt tccccatgaa taagcgtgcc agcaatgttc cgatgatata gatcccagaa
    14881 gcaaaacctg cttggctcaa gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg
    14941 accatcaaca gataataaat caggtagaca ataaaattga tcaatgtgat tccaataaaa
    15001 tatttattaa ataatttctt ttccatgtat gttatacatt ccccttaagc tgaatatgct
    15061 tttaattttt gtatttgaac ttctacatcg tgataattgg atggatagcg atcaacaagt
    15121 tggcgaataa ataaatctct tgtagacata aacagattta aaaattcttc gcattcgttt
    15181 gttggattct catccactgc tttgatcaat tcgatcgagt tttgatacgc atcatttaaa
    15241 aagactaaag aagctttcat atcaaatctt gtttgatatg ctgtttctaa tcgattaatg
    15301 attgtgttca tccagtaatt catgtttttt ccctctattc ttaaaaaatt ttacttctgc
    15361 agtataacga ataaaaaaaa ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca
    15421 aatgttaata tcattagtaa aatacctttg gactttaaaa agttttaaag tactgtatga
    15481 tctttttaga tgaaagtttg gatgggggag catcattgga tagtagagtc ttaaaggaat
    15541 atttggataa aaatgatttt ccgattatac gcaaaggata tcataaatat ttgacattcg
    15601 aagggatgga agattcccat acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta
    15661 tttcaaatga tgggagagaa tttaaccttc gctatatcaa agatcttgaa atcgtctctc
    15721 ttttaaaaga cgaatattca caattcatcg attcgccgta taatattcga gttgaatcag
    15781 aatatgcaga gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc
    15841 caaagctaca acactatgta aaagagtatt accgctatcg tctgctttat tcgatgaaaa
    15901 aaatgcagca aatgctatta aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat
    15961 tatatgattt attcggtgtg cggacagaag aagggatgct gatcgatttt tcgatcacga
    16021 atgaagaaat cgccaaattt tgtggcatct caactgctag cagtgtaaac agaattttaa
    16081 aacaactgaa aaaagatggt attatcaccg ttaaagacca tcatatcgtt attttgaata
    16141 tggacttgtt agaagacaat attatctttt gatgaacaaa gaggttgtga aatcagcgtt
    16201 ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg
    16261 agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgtg agcctgccgt
    16321 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    16381 tgacttgtga ccgaggagtt ggtaggagaa caccgtttac caagaggttg tgaaaaagct
    16441 gttctgcatc aagtaataag aagagccaag taaaaacagt ctcttatgtt tttaacttgg
    16501 ctggacttat caccgtagat gtaagctttt gaacgccgtt tatttgcaac aaaaggggct
    16561 gtgacacgat ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa
    16621 cttctgccgc caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg
    16681 ccgcttccag gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga
    16741 cgatcggttt tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg
    16801 cctgatttca attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga
    16861 gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg
    16921 ccagtttctt cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct
    16981 gatttagaca ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa
    17041 acatcatcta agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa
    17101 gtcgaaatgt agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca
    17161 tttccgactg cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat
    17221 gtagtcgtta cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt
    17281 tccaaagatt ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta
    17341 tctgcatgag tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaac
    17401 tttttcatca tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc
    17461 caagagcggt cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg
    17521 tttgtccatt cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa
    17581 caagccatac cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc
    17641 gcacaccggt caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt
    17701 cttttaattc agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg
    17761 cttggaaccc ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt
    17821 gcacgccttc gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt
    17881 taaaagaaag cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt
    17941 cacctatccg ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag
    18001 catctgtagc ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga
    18061 aaaaagcttt gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt
    18121 cttctttctc gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc
    18181 ccgatagtac gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag
    18241 tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg
    18301 cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt
    18361 cattcatatc ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt
    18421 cttctttgct ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga
    18481 cttcttcttc tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat
    18541 caaaagtcaa cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca
    18601 cacctttcta aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac
    18661 agaatttagg gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt
    18721 gtagcttagg ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc
    18781 cggaattttc tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt
    18841 gttgattttg tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag
    18901 aaagataaga accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc
    18961 ctgcttcatt ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt
    19021 cacgtacgct aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag
    19081 aatcatcggt gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt
    19141 cacgccaaga atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg
    19201 gtacataaga aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg
    19261 ttaggaattc agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt
    19321 tagggattgc gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat
    19381 gtgcccgtaa gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg
    19441 taccgaattt gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg
    19501 gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc
    19561 cattgaacat ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat
    19621 aatcgatgat ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga
    19681 ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt
    19741 tgcggacttt gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga
    19801 cttctagcca ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac
    19861 ccataaaaaa ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata
    19921 ccataggaag atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat
    19981 tgtttaaata cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata
    20041 ataagagata gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc
    20101 gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac
    20161 taaaaaatgc tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag
    20221 gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta
    20281 aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag
    20341 aagatgtgat cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc
    20401 gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg
    20461 tacatatgct ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag
    20521 aaagtgtcat attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt
    20581 cacggactca gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg
    20641 aatttatctt gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc
    20701 gttctgcaga aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta
    20761 gtgaaggaac aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa
    20821 tgatggacag ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat
    20881 tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat
    20941 actgccgtga cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta
    21001 cgatcagaag tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa
    21061 tgataaagat tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt
    21121 atttggaagt cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag
    21181 atgtagcatt tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa
    21241 gaaacgaaat caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg
    21301 tacagcaaac aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat
    21361 ataaagttct gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag
    21421 acttgctgat gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg
    21481 cagctggacc tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag
    21541 caaatgacga agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt
    21601 atgcgcctga tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt
    21661 tgacgcaggc acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag
    21721 aaatcgagtt gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag
    21781 aggaactgtt tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata
    21841 attatgatgc gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa
    21901 aaagaaatgg cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc
    21961 aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat
    22021 gaagaagtcg ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc
    22081 aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa
    22141 ggtttgccag gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca
    22201 attgcgaaac tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca
    22261 agactcgcct tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct
    22321 ttgttaagtg taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa
    22381 gatccctgcg aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa
    22441 gagccgaaag atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt
    22501 ctccatggtg tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct
    22561 ctgttgcagc ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca
    22621 acagagggag aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa
    22681 gtgactcgtc tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt
    22741 actttattga aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt
    22801 cagatacaat ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt
    22861 acaatcgagg ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt
    22921 ttgaaagaag tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt
    22981 gctgaagaga tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg
    23041 gaagcactgc tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct
    23101 ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa
    23161 ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa
    23221 ggtactacgc cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga
    23281 atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac
    23341 cagcgtgtac gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa
    23401 gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa
    23461 cagtatccgc aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga
    23521 ttatccaaga caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg
    23581 ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg
    23641 gtattgaaga tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa
    23701 gaaagcaatt tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt
    23761 atccagtaga agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc
    23821 atcaatttta ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg
    23881 atcgtgtatc agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt
    23941 tttgaagggg agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg
    24001 ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc
    24061 gaaaagaaag aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc
    24121 gatcagatca gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa
    24181 gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa
    24241 ttcttggaag agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga
    24301 attctcccta ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa
    24361 gcactgatcc atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct
    24421 ttcttaacaa acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat
    24481 gctaaggact tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt
    24541 atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga
    24601 ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa
    24661 tatggcaaag tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa
    24721 ctacgcagca atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt
    24781 tagcttgccg caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc
    24841 gatatagaga tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata
    24901 gtgacaaggt attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta
    24961 aaaaagagat tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt
    25021 cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa
    25081 tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca
    25141 catttgatcg aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag
    25201 aattggtaaa agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg
    25261 tccgcgatga agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt
    25321 cttctttttt aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat
    25381 ctttgaatcc aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg
    25441 agagcgaaga atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta
    25501 cacctgatgg taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc
    25561 ggctgactgg acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc
    25621 aagtaggtga gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca
    25681 gagttgagaa atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta
    25741 gaaaaaaata caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg
    25801 aaccaagttg cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg
    25861 aatcttgaaa aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga
    25921 agggacaatg acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga
    25981 tgaccaataa caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga
    26041 gattgtgaca gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat
    26101 atcttgtacc gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc
    26161 tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc
    26221 attttgaaat ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc
    26281 ctcaattgat tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa
    26341 tgccttccat cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt
    26401 ctgtgatctc tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag
    26461 tgcctcaacc atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa
    26521 cattgaaaaa gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga
    26581 tcactgctac attgacgaat tttttagaaa tttttggaaa tcgccaagtc gttttgtgtc
    26641 gggaattgac aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt
    26701 atatcgaaga acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg
    26761 aaatgccagt gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa
    26821 agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc
    26881 tgaaaaaaca agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc
    26941 tataatgaaa gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac
    27001 gaatgttctt gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata
    27061 atgatacatc aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag
    27121 aacgagatca tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca
    27181 ctggtgggcg tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt
    27241 cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg
    27301 accatcataa atacagcgat atttccaaac aagtacggga agtattctat cgttatacag
    27361 atattgtgga accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca
    27421 attgcaagtc agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc
    27481 agctaacatg ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact
    27541 atcaaaaacc tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag
    27601 ctttgccaat tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc
    27661 tagggattta tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt
    27721 ttggaaaaat gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa
    27781 acgttacaag agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa
    27841 cggaagaagc agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga
    27901 gaagagtcca aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta
    27961 ctgtgaccaa aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc
    28021 aagcaagcct tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga
    28081 ttactttgac gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa
    28141 atcaagaaat ataaaaacag ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa
    28201 gctgttttta tatcaagtct tgctttttga tttgttcata ggtttctgtt gcatccgtac
    28261 atttttccca aatcacaact tgattggatt ctaaagattt ttggacgtca atgatccgct
    28321 ggttgctact tccgcgaaat tgtagtttta ggtcacgttt gcttaattca aagcgtccgt
    28381 caacaaggat atcgatttgc gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt
    28441 catcaaaggc atagcctgtc catgaccaga tatcttttgt cgtaccgaac tcttttcgga
    28501 tacgtcgtac gacttgcaga caaacatctg tattcaaaaa aggttcgcca ccaagtaaag
    28561 ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg
    28621 tgaatggttt gccatatcgg aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa
    28681 ataagcagcc actaacgtaa agactgttgc ggacaccttc accatcgaca aaattaaacg
    28741 gtttgtagtc agcaatatag ttttggctgt agtcttttgc taaccattct tttggttttg
    28801 gatttctcat aaaaaaactt ctttcattta aggttcaaag atatagctat tgaagataaa
    28861 atctactcga ccacataaat aagaacaaac tgacctaaat ccattgttca agaaagcttg
    28921 ttcttatttt gatagagggc gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa
    28981 agacttgtca tagcctctta ttctgaataa acggtgttca aaagcttgca tctgcagtaa
    29041 taagcccaac taaacgaaaa atatgaggaa ctatttttgt ttagctattc ttattacttg
    29101 atgcaggaca gctttttcac aacctcattt catgtgtttc acacgtgagg aaatttcttt
    29161 atggcggcca tgtaccatcg gacgagcttg cggatttcct aaatagccac aagtacgttt
    29221 gactacatca catgtttttg gatcatgatt tccacattct ggacattcga accctcgttc
    29281 tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt
    29341 accaagatac ccgattttgt catatgcaaa atcccataca gcttctaaag ctttcggatt
    29401 ttgttggagc atagggtatt cacagtagtg gatgaatcca cctgaacaat attttggata
    29461 atccttctca aattccaatt tttcaaatgg cgttgggttt ttacgcacat catagtggaa
    29521 actgtttgta taatattctt tatctgtgat attttcgatg atgccgaatt tttctgtatc
    29581 caaacggcag aaacggtcag ttagactttc acttggagtt gaatagacac tgaaatgata
    29641 accatattca tccccccatg catctgcatt agcttttaat tctttgacga tatcaagtgt
    29701 gaaagctttt gcttctgggt ttgtttccca ttcgccaccg aagaaagctg aagcaacttc
    29761 atacaatcca atgtagccta acgatacagt agctcgacgg tttttgaata attcattgac
    29821 agaatcggta cgtgaaagac gtttcccgaa agaaccatac atatataaaa ttggcgcatt
    29881 tgctggaatg gcttctttac aacgttctac acgatagaga agcgcatctt tcatgatttc
    29941 taagcggtca gacagtaact gccagaattt attctgatct ccttttgatt cgatcgcgat
    30001 tcttggcaaa ttcaatgtaa caacacctaa attcattcgt ccgacattta cttcttgacc
    30061 attttcgtct ttccaccctt gcaagaatga acgacagccc ataggtactt tgaaacttcc
    30121 agttaattca acgattttat cgtaattcag aatatcagga tacattcttt tcgttgcaca
    30181 ttccaatgct agttgtttga tgtcgtaatt aggatcttca gggtttaagt tcacaccttt
    30241 tttcaaagtg aagatcagtt tagggaagat agctgtccgc ttttcactcc ctaatccatt
    30301 gattcgaatc tgtaaaattg ctttttgaat ttctctttca aaccagtttt ctcctagtcc
    30361 aaatcctaat gaagtgaaag gagtttgtcc atttgaagta aataaggtat tgatttcata
    30421 ttccaaactt tgcatagcat cgaagatatc ttttttggtt tttgcttttg catattcttt
    30481 ttggcgttct ggcgaatcga tccattcttc cgcgtctttt agatgttttt tatagttcaa
    30541 ttctgcaaaa ggagcaagca attcatctgt tcggtcggca gaacagccac catattgact
    30601 tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga tagattttgg
    30661 cgattcgact tcggcattgc cgattttgaa cccattgttc agcatgcctt tgaaatcaat
    30721 caagcagcaa ttcgtcatcg gtgtataagg atgataatcc aaatcatggt aatggatatc
    30781 gcctttttga tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga ttgattttcc
    30841 aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt
    30901 ttcgttcacg acagtttggt ccttgttgat tagcttttca atcgaaaagt tgatatctgt
    30961 tgctttactg cgagcaaaat ctcgtttggt acgataattg atgtactctt ctgctaattc
    31021 atattcgttt ttttctagta atatatgttc cacgatgttt tgaatctcat agattttgac
    31081 attttcagca aaccggcttt tgatttcttg gacaaccaag tctgcaatca tttgaatttt
    31141 ttcataagta agtggtgata atgagccgtg aattttcttt tctgctttga tcagtgcttc
    31201 attgatcttt tgttcatcga aatcaacatg gcggccatcg cgtttgatga ctttcatctg
    31261 ggatgtctct ggctgctgag taacgacagt tttttcttca taatttactt tcattatctt
    31321 caactcctgc taaattgttc ttgtctatca taaaacaaaa tcggggaagg agcaactaaa
    31381 tatcggctat atatagtatt caatgtgctg tataattgga tttctatact atatgtagtg
    31441 tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt ggatttgttt tttgcatatt
    31501 taattttgct gaataaaata attgaataaa attttttatt cagtccaagt aaaagtgaca
    31561 aaaacataag agcaaattat tcgtagatat gctactatgc aaataaatag aaatgaaaaa
    31621 tgaaagagga ggagaagaat tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta
    31681 cggcagcaaa ctttctcccg tcaaagcctt agataatatc tcattcacag tagaagaagg
    31741 cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag acaacgctga tgaatatctt
    31801 gtccacgatc aatttgccta cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa
    31861 aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga
    31921 gttcaatttg atcgataccc taaatgcaaa agacaatatc ctattgcccc tagcagttga
    31981 acggatgact aaagaggaaa tggaaaagag agtaagacat gtagctcaat tgttgaatat
    32041 agaagaactg ctcaaacggt atcccgatga actttctgtc ggacagagac aacgaattgc
    32101 agctgctaga gcacttgttg ttcagccaca agtgattttt gcagatgaac caactggctc
    32161 tttggactcg aagtcggcta cagaattgtt gaactacttg aagaccatga atcaaaaaga
    32221 aaaagcaacg atactgttag tcacccatga tccatataca gcaagctact gcgatagaat
    32281 tttatttatt aaagatggtg tgattttttc tgaagttgtc agaagaggga caagaagaga
    32341 gttctttgag aaagtaatag acatgcaggc gacgattggc ggaggaggaa aaatgaatgc
    32401 tgtataaact agccgttaaa agtttttttc ggcagtcacg aggctatctt gtttattttt
    32461 tcagtttgac tttatccgta atgatctatt attccttcag cgcaatgacc tacgatcaat
    32521 ctttagtcag aagagcaagt caagatactt cgatagatgg tggattgagt tttggtggat
    32581 ttatcattac tgttgttttg ctttttttcg tattcagtgc caaccgattt ttcttgaata
    32641 gaaggcaaaa ggaaataggc atctatcagc tttttggtat gaataaactg cagatatcag
    32701 gtatatatgt gttagaaatc atgataattg gattgttcgc ctgtatttct gggatattac
    32761 tcggtattat tttttccaaa ctcttttcaa tgattttggt tcgaatgatg gatatggatc
    32821 ttactagtcc attttttatc tccattccat cagtagtaga tactttattt gcttttttca
    32881 tcatcctttt agcagtatct gtatattcaa tatggaaaat atggcgctat ccaatgattc
    32941 gaagttttgg agaaaaagaa caagcagata gttcaacaat gcgcttcagg acaaggcacc
    33001 gattactcgc agtcatcggg ttactacttt taacgagcgg atattttgga gctagtcatt
    33061 ttagggaaat cacttcatgg atgatcagtt atcattatct tgatacatta gttttctttt
    33121 ttcctttttt aatttttttc gtttgtgtgg tcggtactta tttgtttttc tgttatacat
    33181 ttcggttatt tttgatcata ttgagtaaat ggaagattta ttactatggt atcaattttt
    33241 tgataatggg taacacgcaa gttcatttgc tcaaaagttg gcgtacaaat tctctgatta
    33301 cagtcattct cggaatttct ttaatgacaa ttggcggcac ggctagtatc tctactattt
    33361 tatcctacca ggagaaagtt tcctccccga tggactatca gtttgatgtt gagacagaga
    33421 aaaaaatcaa gccgattcta gcagaggaag ggcagaagat aactaaagaa atcaccttgc
    33481 attataaagt cattgggagt atctatagtc aaaatctcga agaacctatg tttcaaactg
    33541 ctaatctgat cacggaaaca caatatcaag ccttcagaaa agttaatcca gatttgccga
    33601 acatttcttt gaaatcacct ggtcatactg ttttgcttga tcaaatgcaa actctttttc
    33661 ggaatatttc attatatgga tctagtattg gactgccaga taatcaagaa ttgaagattc
    33721 aacaaatgct tccaagtgta ttaggtgacg aaacaatggt atatctaagt cctgtactga
    33781 ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag
    33841 atgtatcagg tggaaataat gaaaaaatag atgaacgact gagcaatgac ctgtcgataa
    33901 agtggctcaa tgctgtttat tattcttatg atatcgtaaa tggaagtcta caaggagaaa
    33961 ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc acaatttagc caagaaagct
    34021 ctcgtttgaa tttctcttcc agctattcga aattgcgtgc agttaatcgg cgaatcggta
    34081 tcaatatttt tgtcacacta tttgtcggga tgatcgtgat tattgctact ggtagtattt
    34141 tgacagtccg gcaattagca gaagcagagg aagaaaaaga aaattatcac ctattgacta
    34201 agctggggat tccacaaaga aggatcagac ggatgatctt cgagcaaaac gcacttacct
    34261 tctttccacc catgatatta gggatcactc acgcagtatt tgcaatcaat gtcttcactc
    34321 agtatataaa aactgccgat tactggttgg cttatcttgt gagtggctta ttgatcctta
    34381 tctatctttt gctttatgtc attacgagtc agttatattg tcggattatc gaggaatgat
    34441 atacggaata agacaagata ataaat
//
LOCUS       DAZBTH010000019        34175 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000019 DAZBTH010000000
VERSION     DAZBTH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.019"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..922
                     /locus_tag="ACHGNW_000907"
     CDS             47..922
                     /locus_tag="ACHGNW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN6925242.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1067..1219)
                     /locus_tag="ACHGNW_000908"
                     /pseudo
     CDS             complement(1067..1219)
                     /locus_tag="ACHGNW_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1487..2818)
                     /locus_tag="ACHGNW_000909"
     CDS             complement(1487..2818)
                     /locus_tag="ACHGNW_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6925243.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2967..3680)
                     /locus_tag="ACHGNW_000910"
     CDS             complement(2967..3680)
                     /locus_tag="ACHGNW_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6925244.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3855..5279
                     /locus_tag="ACHGNW_000911"
     CDS             3855..5279
                     /locus_tag="ACHGNW_000911"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6925245.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5578..6285
                     /locus_tag="ACHGNW_000912"
     CDS             5578..6285
                     /locus_tag="ACHGNW_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6925246.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6272..6595
                     /locus_tag="ACHGNW_000913"
     CDS             6272..6595
                     /locus_tag="ACHGNW_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6925247.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6684..7721)
                     /locus_tag="ACHGNW_000914"
     CDS             complement(6684..7721)
                     /locus_tag="ACHGNW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6925248.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8042..9025)
                     /locus_tag="ACHGNW_000915"
     CDS             complement(8042..9025)
                     /locus_tag="ACHGNW_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN6925249.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9440..10210)
                     /locus_tag="ACHGNW_000916"
     CDS             complement(9440..10210)
                     /locus_tag="ACHGNW_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925250.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10203..11138)
                     /locus_tag="ACHGNW_000917"
     CDS             complement(10203..11138)
                     /locus_tag="ACHGNW_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925251.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11454..13103)
                     /locus_tag="ACHGNW_000918"
     CDS             complement(11454..13103)
                     /locus_tag="ACHGNW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6925252.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13220..15823)
                     /locus_tag="ACHGNW_000919"
     CDS             complement(13220..15823)
                     /locus_tag="ACHGNW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6925253.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15835..16884)
                     /locus_tag="ACHGNW_000920"
     CDS             complement(15835..16884)
                     /locus_tag="ACHGNW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6925254.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(16910..17224)
                     /locus_tag="ACHGNW_000921"
     CDS             complement(16910..17224)
                     /locus_tag="ACHGNW_000921"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN6925255.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17240..17686)
                     /locus_tag="ACHGNW_000922"
     CDS             complement(17240..17686)
                     /locus_tag="ACHGNW_000922"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN6925256.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17687..19609)
                     /locus_tag="ACHGNW_000923"
     CDS             complement(17687..19609)
                     /locus_tag="ACHGNW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6925257.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19821..20717)
                     /locus_tag="ACHGNW_000924"
     CDS             complement(19821..20717)
                     /locus_tag="ACHGNW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6925258.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20710..21390)
                     /locus_tag="ACHGNW_000925"
     CDS             complement(20710..21390)
                     /locus_tag="ACHGNW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6925259.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21391..22173)
                     /locus_tag="ACHGNW_000926"
     CDS             complement(21391..22173)
                     /locus_tag="ACHGNW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6925260.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22166..23083)
                     /locus_tag="ACHGNW_000927"
     CDS             complement(22166..23083)
                     /locus_tag="ACHGNW_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6925261.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23247..23501)
                     /locus_tag="ACHGNW_000928"
     CDS             complement(23247..23501)
                     /locus_tag="ACHGNW_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925262.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            24358..27585
                     /gene="fss3"
                     /locus_tag="ACHGNW_000929"
     CDS             24358..27585
                     /gene="fss3"
                     /locus_tag="ACHGNW_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN6925263.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27698..28012
                     /locus_tag="ACHGNW_000930"
     CDS             27698..28012
                     /locus_tag="ACHGNW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6925264.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28025..28399
                     /locus_tag="ACHGNW_000931"
     CDS             28025..28399
                     /locus_tag="ACHGNW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6925265.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28409..28744
                     /locus_tag="ACHGNW_000932"
     CDS             28409..28744
                     /locus_tag="ACHGNW_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN6925266.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28827..30167
                     /locus_tag="ACHGNW_000933"
     CDS             28827..30167
                     /locus_tag="ACHGNW_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN6925267.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30245..30919
                     /locus_tag="ACHGNW_000934"
     CDS             30245..30919
                     /locus_tag="ACHGNW_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6925268.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            30912..31076
                     /locus_tag="ACHGNW_000935"
     CDS             30912..31076
                     /locus_tag="ACHGNW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925269.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31152..32294
                     /gene="dcm"
                     /locus_tag="ACHGNW_000936"
                     /pseudo
     CDS             31152..32294
                     /gene="dcm"
                     /locus_tag="ACHGNW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32284..32574
                     /locus_tag="ACHGNW_000937"
     CDS             32284..32574
                     /locus_tag="ACHGNW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925270.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32833..34017
                     /gene="mobT"
                     /locus_tag="ACHGNW_000938"
     CDS             32833..34017
                     /gene="mobT"
                     /locus_tag="ACHGNW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN6925271.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34014..34151
                     /locus_tag="ACHGNW_000939"
     CDS             34014..34151
                     /locus_tag="ACHGNW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN6925272.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10636 a         6586 c         6238 g        10715 t
ORIGIN      
        1 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
       61 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
      121 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
      181 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
      241 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
      301 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
      361 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt
      421 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
      481 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
      541 gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc
      601 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
      661 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
      721 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
      781 tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga
      841 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa
      901 agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa
      961 tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa
     1021 cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac
     1081 aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt
     1141 ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc tgacaacaac
     1201 tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa cgattaataa
     1261 tattaacagt ctttattttt caccaccaaa aacctatctt tgcatatact aaatagacgt
     1321 atatacacag ataggtttgt cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg
     1381 tttttctaca tgttcgtact tatcaaaaaa tatgatcctt attgaaatta ctggctattt
     1441 atattccaac gacttcttgt gttaattttt gttatctcat agtaaattat gtttcatttt
     1501 cacttaattt agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac
     1561 ttgcggctat aattaatatt tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa
     1621 atcccgatat aactggtggc gtagtccaag gaatttgcac tactacatga ttaattaatc
     1681 ccacagcagt tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc acaaagggaa
     1741 taatcagact gatattaaat acaataggca atccaaaaat aataggttcg cttatattga
     1801 atatagcggg agttaccgat aatttagcaa agtccttata atcttttcta cgactgaaga
     1861 taaatattgc aatcaataaa gcaatcgtat ttcccgatcc acccattacc gcaaaggtat
     1921 ctttaaaggc catattgata atatgtggaa tttgctcttt gtttgcataa gctgccatat
     1981 tttcttgcat attctgaatc aaaaacggat ctaacaatga acctgatatt actgcttgat
     2041 ggataccaaa tccaaaaaat agattagcaa tagatgtaat taacaaaaat cctggtaaac
     2101 cagttgtaat tttacttaat ggctctgtaa ctatcgtggt aattagctta tttatatcca
     2161 tattaaacaa ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc gtaataatga
     2221 ttgggattaa tacattaaaa gactttaaaa cagcgggagg aatatttccg gatagctgaa
     2281 tctgtagttt cttattatta gaaagtttaa taaataattc tgtagccgct aatccaacaa
     2341 atatacctac aaacattcct gaagctcctg tataggatat aggtatcaca cccgtcactt
     2401 caacagcttt atccgcattt tcaggagtaa acatcatctg cataggtgtt aatacaacaa
     2461 atgtcgaaat agcaactaat accgctgcaa tatgatttgt ataatttctg cttgcacaca
     2521 tatgataaga tactgctgct acaactaata atgtagttat gcttaatgta ccattggcaa
     2581 ttgaagtacc aatttgttgt aacgtggtca atgtactagt atcgacaatt tcccccatga
     2641 atcctgttgg ttctaagatc actgaattaa taagtgtcac aaatcctgct aacatcataa
     2701 atggcataaa tgtagcaaac gcatctcgca aagttcttaa atggatctga ttccccaact
     2761 tagtcgcaac tataccaaat ttttcaaata caatgtcctt cctgctactt ttgcccatat
     2821 tttttctcct ttatgcttat tgtaatcgat tactttttcc acccaaataa tacatcactt
     2881 atcaaaaaaa gtaaatactt ttaattaaat atatatatct tttttagtat aaaaaaataa
     2941 gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag
     3001 tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga tagagcaaca
     3061 actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt tgcgtcaaat
     3121 tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag acccagttca
     3181 tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc tggtatgact
     3241 gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa acggcttcgt
     3301 acaatattat atacatagtc ttttacactg atacttaatt tatcagctac ttttttcgat
     3361 ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc aaccacgcta
     3421 aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct gacgacttca
     3481 gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc
     3541 tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt aggaagtttc
     3601 tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta aatttccata
     3661 tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc atctatactt
     3721 taacataaat aaaccgataa aatccacatc aaaattatac acacaataaa aaagatatat
     3781 atatttaaac aaaactattt actttaagtt gtaagcggtg ttatcattaa gtggaaacag
     3841 gaggtaaaaa aattatgaca actatgaaaa agctaacttt tccagatgat ttttggtggg
     3901 gttccgcttg gtcagctgag caagcagaag gaaaaggaga aacaggaaaa gcagagacta
     3961 tctgggatcg ctggtttaaa gaacaacctt accggtttta tcaacgaatc gattcctcta
     4021 ttacaaccga tcacatccat caatataaag aagatataga actaatgaaa aaaactgggc
     4081 ataactcctt tcgcgtttct atttcttggg cacgaatgtt tccaaatggc ggtaccggaa
     4141 aagtaaatcc caaagccatt tctttctatc gaaaattatt ttccgaaatg aataataacg
     4201 gaatcaaagt atttgccaac ttatatcatt ttgacatgcc tgctactctt caagataaag
     4261 gaggatggga atcacgtgaa gtagttgatg cttatgttca ttacgctgaa acttgtttta
     4321 aagaatttgg tgacttagtt tgtcattggt ttaccttcaa tgaaccactt gggcctattt
     4381 taggtactta tcttgaagat tttcattatc caaacctcat tgactttaaa cgcggagcgc
     4441 aagcagcatt cttcactata cttgcacacg caaaagcaat acaagtattt aaagaactag
     4501 ctcttcctag taaaataggc gttattctaa atttgagtcc tacttttcct cgtagtcaaa
     4561 atcctgctga tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg agctttttag
     4621 atcctatggt taaagggaat ttttctaaaa agcttatcct gttattacaa gaatataatc
     4681 agcttcctga agattatacg aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa
     4741 ttcttggact aaactattac gagccacgtc gcgtcaaatc tcgttcttct atggtggata
     4801 catctagtcc atttttacca gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa
     4861 tgaacgaata ccggggatgg gaaatttacg aaaaaggaat ttatgatttg tgcatggata
     4921 tcaagaacca ttatggaaac atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg
     4981 atgaagaacg tttcatggat gatacagggc aaataattga tgattatcgg attgagtata
     5041 tcaaagatca tcttgcttac ttatggaagg caataaatga aggatgtaac attaaggggt
     5101 atcatctttg gacatttatt gattgctggt catggatcaa cgcctataaa aaccgatatg
     5161 ggctggtttc actcgacctt actactcaaa aaagaacaat taaaaaaagt ggcgaatttt
     5221 acaagcaatt aagtgataca aatggattta gatacgatgt agacttgtta gttgaataat
     5281 attctaaaat tccacttgaa gtgaacacat tagaaaacaa gaataatgag aaataccaat
     5341 aacaaatact ttttcttcat acttcattta tacttgtcag tataagagac tgtgacaaat
     5401 gtagtgatcc tcagaagtta ggtctggaaa tctaacttga gaagattttt ttaaactacg
     5461 tccagtcttt tttatttgtt taaaattgaa gttgacaagt cttcattaaa aatttagaat
     5521 attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg
     5581 gatgaaaagt tagatataaa aactgcgatc aaagacacgt tacctaccgt ttttggttat
     5641 atcggtattg gacttgcatt tggtatcgtt gggaaagctg ccgggtttca tccgctggtc
     5701 gtcacgttga tgtccctgct gatctatgct ggttctgccc aatttatcac ggtcagcatg
     5761 cttgctagcc atagtccact gctctccatc gttctctcga cctttctagt caattcccga
     5821 atgattctga tgagtatgac gattgctcct tatttcaaga aaaatcggct gcttcaaaat
     5881 ctgctgattg gcacattgct gacagatgaa agtttcgcac tgggaatgaa taaattgaac
     5941 tatactggac aaaagttaaa ctttcgttgg atgaatacag ctaattggat ttcttatttg
     6001 acgtgggtgg gttcttctct tgttggcgca cttttaggga attttattac tgatccgaaa
     6061 aagtttggtt tggatttcgc catcgtcgct atgtttatcg ggttacttta tcttcaagtc
     6121 atttctgata gaaacacctc taaacgatta cagttgatcc tgattggtct cactttgatt
     6181 ctggtatatg tcggactgat ttttatccca agtaacttag tgattttagt tgtaacactg
     6241 attgggtgcg gattgggggt gtggatcaaa catgccttct tttgagttta cattgctgac
     6301 tattttaggt tgtactttag caacttggct ttctaggatc ctaccatttg tcttactgaa
     6361 aaaattcgat ttgccgcaac cgttgcttga gtatttaagt tttgtcccga tcgtcattat
     6421 gtctgcttta tggttcagca gtctatttac ccaaaatatc ggacatctgc cgcagatcaa
     6481 tctcgaaaat gctttggctt cagttcctac acttttagct gctattcttt caaaaagctt
     6541 attggtgatc gtcttggtag gaattctttc cctttcgttg attcgcttat tataaataaa
     6601 aaaacaagag cttatggcaa attactgtca tgcgctcttg tttttatcaa agtcaaataa
     6661 gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac cactaacaac gacttttcct
     6721 aatatatgtc ctgtttctac gtctttggtt gcttgtttca agttcttcgc attcatccca
     6781 tctgaataga ccttgctcaa tgtactgcgg ataatccctt catttgctaa agcagcaatg
     6841 tgcgctaaag cttctccttg cgtttcgatt tcatagttat agtctgtttt agcgaacata
     6901 tactcccagt cgaagctgat agattggttt ttccaaacac ctatatctaa cggctctttg
     6961 atccctacga tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag
     7021 tagcgggtaa tatcgaataa tacagcgaca tactctactt gatcgattcc taattcacga
     7081 aaacttttac ttaagtcgtg atgataatcg attggatagt ctacacctgt ttttttcagc
     7141 cattcaaaat tcttcggact tgcagtcgcg tagacttcta ggccagccca atgcgctagt
     7201 tgattcaaaa tcgaaccgac gcccccagct ccgttgatca ctaagatttt tttcccgcgg
     7261 ttggcatttt cttctggagt cagaccaaat ttttcaaata acaattcata agcggtcaat
     7321 gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat tttttggtgc aagagcgaca
     7381 atacgttcgt caaccagttg gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg
     7441 taaaaaacgc gatcgccgac agaaaatttt tgcacttcat cacctacagc aacaactttc
     7501 ccgactccat caaaccctaa gatacgtaaa gcatttctta tcccgttatc ttggcgaagt
     7561 ttcgtatcga caggattaac agatactgcg ttgactttta ccagaagatc ccgtgaactg
     7621 ggtacaggta tctgttcttt ttcatcaata aagctttttg gatcatcgat cgacaaccct
     7681 tcataaaaac cgactacctt cattaattcc ttttctgaca tacgaattcc tccttagctt
     7741 catcaagttg gagcatccat acagttttca tttcaatata tatagtatgc tatttgttcc
     7801 acttttattc atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta
     7861 aaaaagaggt tgcgacaaga cttttgtcac aacctctttt aaacggtgtt caatcaactg
     7921 accctcggta ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt ttggctgttc
     7981 ttattacttg atgcaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa
     8041 atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag atctctcgta
     8101 cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc
     8161 ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg
     8221 aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat
     8281 gttccctcgg ttctttttca aatgcataag gattcttgaa aatcccccgg ccaatcatga
     8341 tgccatctac gccatatttt tcggccaatt cttctcctgt ctggcgatct gggatatctc
     8401 cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg taaagcaatg attttaggga
     8461 tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa
     8521 gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg
     8581 tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagcc gcttcaatca
     8641 attgtgccgc tacttctggg cgaaggatca agccactgcc ttttccacgt tctgctacat
     8701 tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa
     8761 tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt
     8821 catcttctgt aaaggtcaat cttcctcgta cactgtcttt tccatctgga tgacagaaac
     8881 tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat
     8941 ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca
     9001 gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag
     9061 tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga
     9121 acagcttaaa agatgtttct gattgagcaa aaaaagactg tgacaaaaat cttatcacaa
     9181 ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
     9241 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     9301 ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca
     9361 aacgacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc
     9421 tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag
     9481 taacggcgta caaaacaacg atgaatagca caagtacgat catcgatgtt gctactggca
     9541 catcgattgt tccaaggaat ccgtaacgga aaccggagat catataaacg atcggattga
     9601 ctttcgaaac agcttgccaa aatggcggca acatagagat ggcatagaaa acgccaccta
     9661 gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcgtca taagactgag
     9721 caaaaatccc gttgagcagt cctgctaacg aaaacaagat tgccgtcatc aataatgtaa
     9781 tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta
     9841 cgagagagcc gacgagaata ctgcgaccca ctccgccaat cacaaagccc caaatgatca
     9901 catgcgtagg aacaggcgct accaagattt cttcgatgtt tttctggaat ttttgagaga
     9961 agaaggacga agaaacgttg gcatacgaac tagtgatagc agacatcata atcaaaccag
    10021 gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgacca ccgatcattt
    10081 ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg cggaaccagt gtttggaccc
    10141 agatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa
    10201 gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa aaacagttct
    10261 tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct tgtcagctga
    10321 tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgttcatct
    10381 tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct catatggcgc taaatcaaag
    10441 ataaatgttt caaattgcag tttggataga agctctttca tactagtatt ctcgatcagt
    10501 tcacctgatt ggatgatccc gatgttgcga caaagcattt cagcttcttc taaataatgc
    10561 gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc ggagaaactc ccacatctcg
    10621 cgtctcaatt caatatcgac tcctgctgtt ggttcatcca agatcagcaa acgtggttca
    10681 tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca
    10741 cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc
    10801 gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt
    10861 tcaaagggat tgaaattgaa ctcttgcggg actaatccaa tctgttgctt tgctcggact
    10921 agatccgtat ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa
    10981 gaagtaatga ttccgatcgt tgtagacttc ccagctccat ttggtcctaa tagcgcatag
    11041 aaatctcctt ctttcacgga aagatcgatt cctcgcaatg cttcgacccc acttccataa
    11101 acctttttca aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga
    11161 catgattctt tattgtatca tggaatacag cagatgaaaa aacaacttgg ttccagcgaa
    11221 aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca
    11281 gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt actattcata tgtgcaacaa
    11341 aaggagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    11401 gcggaagcaa aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc
    11461 aatacatgcc actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact
    11521 aaagtctctg ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt
    11581 ccttctttta atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag
    11641 tggacattct cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca
    11701 aatgctaaag atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt
    11761 tcgatcgtat ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa
    11821 gccacttggt ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct
    11881 tcaaataaat catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac
    11941 tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg
    12001 acatacgtag attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg
    12061 accggattca ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg
    12121 ccctcagtaa actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca
    12181 gcggctactt ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt
    12241 tctaatttag tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca
    12301 tattcaaaaa tcaactctgc ttcactccca ctcttcatac gaaatgaaaa acctaattta
    12361 gggatgtctc ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt
    12421 tttgctagct gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct
    12481 gtttctagtg tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt
    12541 gccaaataga ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc
    12601 aagccaagaa aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga
    12661 tattgttttg caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt
    12721 tctctcagtt gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca
    12781 ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta
    12841 ctgttgattc cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt
    12901 tcgctatccc atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct
    12961 ttatcaaatc tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat
    13021 ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa
    13081 acgataaatt ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata
    13141 gcactgttgc gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt
    13201 tttcttgtat attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag
    13261 gaattgttgt taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga
    13321 tagcctcagg tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt
    13381 gttcatgaaa ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca
    13441 atgaaaattt acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc
    13501 tgtgaataaa tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt
    13561 ctgcctgttt tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag
    13621 tgactttata ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat
    13681 ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc
    13741 ctgttgttga aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac
    13801 catctgcata aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata
    13861 tttctaaatt aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag
    13921 gggtattttc aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa
    13981 cattaattgc taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt
    14041 caacgatttg actttctccc agactgaacg atatttcata agatatttct tccatttcgg
    14101 gtttttcaga taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta
    14161 atgtttcttg taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg
    14221 tatctccttc taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat
    14281 gaacgaaatc aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag
    14341 aaagtttata tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt
    14401 tttcattatt tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta
    14461 tttttatgtc aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca
    14521 tccgataagc atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac
    14581 tacctttgaa gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat
    14641 aattctcagt cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag
    14701 ccatccgttt tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc
    14761 gatggaaaat atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct
    14821 ggttttgcaa aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat
    14881 ttctttctgc aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa
    14941 aattttctat tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa
    15001 ccggctgacg aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta
    15061 cataagaagg ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta
    15121 cgacttgatc aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat
    15181 ctttgttttt tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa
    15241 attcaggaat atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa
    15301 aattcatggc ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa
    15361 ccatcgtttc aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca
    15421 aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg
    15481 gatctccata tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt
    15541 cagcatccac taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg
    15601 ttttcatcag tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga
    15661 cgattgaagt ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta
    15721 gaacttcagt aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt
    15781 ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt
    15841 tccttcttct actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt
    15901 gaccattaac gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt
    15961 cgtaacaaaa ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa
    16021 tccatttaga ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg
    16081 atctttcacg gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc
    16141 tgtctttgca ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa
    16201 aaacattcca aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct
    16261 tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat
    16321 atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat
    16381 gccttctagc caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata
    16441 ccataatatg attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag
    16501 actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc
    16561 taatccagca accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt
    16621 gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat
    16681 catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt
    16741 atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc
    16801 tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc
    16861 tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa
    16921 ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta
    16981 ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa
    17041 atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag
    17101 ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag
    17161 cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg
    17221 tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg
    17281 aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa
    17341 tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa
    17401 ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg
    17461 ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg
    17521 tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa
    17581 acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat
    17641 ttaaatctat cactatatta tttatattga ttttttcact gatcatttat aagccttctt
    17701 tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat
    17761 gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt
    17821 cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg
    17881 gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag
    17941 caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc
    18001 cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt
    18061 gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag
    18121 catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta
    18181 acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta
    18241 taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta
    18301 tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat
    18361 atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg
    18421 attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct
    18481 cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga
    18541 ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat
    18601 tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa
    18661 ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta
    18721 agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt
    18781 catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat
    18841 tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc
    18901 tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca
    18961 attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat
    19021 ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac
    19081 tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt
    19141 tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat
    19201 gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt
    19261 ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa
    19321 acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata
    19381 aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca
    19441 tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg
    19501 ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa
    19561 tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct
    19621 cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg
    19681 attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata
    19741 tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt tttctactag
    19801 ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc
    19861 ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa
    19921 agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg
    19981 aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga
    20041 atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa
    20101 ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta
    20161 tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa
    20221 gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt
    20281 tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca
    20341 acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc
    20401 agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg
    20461 acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt
    20521 tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg
    20581 actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat
    20641 ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc
    20701 aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg
    20761 tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc
    20821 aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg
    20881 atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc
    20941 attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg
    21001 cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa
    21061 aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga
    21121 aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg
    21181 tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat
    21241 caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag
    21301 aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc
    21361 gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa
    21421 cattcctaga gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc
    21481 ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca
    21541 ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc
    21601 catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa
    21661 agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag
    21721 aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta
    21781 agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac
    21841 gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat
    21901 cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag
    21961 aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc
    22021 cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata
    22081 aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac
    22141 ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga
    22201 aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta
    22261 ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc
    22321 tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg
    22381 tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata
    22441 attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt
    22501 catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga
    22561 tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa
    22621 tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta
    22681 aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt
    22741 cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg
    22801 cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg
    22861 cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca
    22921 taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca
    22981 ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg
    23041 ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt
    23101 attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa
    23161 gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt
    23221 atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc
    23281 gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat
    23341 tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa aattagactg
    23401 tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca
    23461 agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc
    23521 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
    23581 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
    23641 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
    23701 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
    23761 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
    23821 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
    23881 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
    23941 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
    24001 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
    24061 gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt
    24121 ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa tgtttgtgtt
    24181 ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa tttttaggct
    24241 cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac tttataaaca
    24301 aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga acatttcatg
    24361 tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc tgttcaagca
    24421 attgttggag caattgctcc tacgattact tttgctgatg aaattaccca tccacaaacg
    24481 gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt tagtgatggt
    24541 cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt taaacaagat
    24601 attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg ctatgagaaa
    24661 aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg gaaaaaagta
    24721 ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga agttaatggg
    24781 tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc agccattgag
    24841 gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca taatagtacg
    24901 gctaaaacag tattagggca atcgactact gtgacggata cgaataatct gaatttgtca
    24961 gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa tggtaatcag
    25021 ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct taagaaatct
    25081 gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt aatggccggg
    25141 gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga gggttcttta
    25201 aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt tttccattta
    25261 gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg cattgctaca
    25321 ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt accggcgcct
    25381 tatacgattg ataccacccc catgactacc accattaaag caggcgaaac catttacgtg
    25441 acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg agttgaaaca
    25501 gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc cattcgcaaa
    25561 gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg tcatgcggaa
    25621 acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac cgaaactaaa
    25681 gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa gtatgccaat
    25741 caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt cactggcgaa
    25801 accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa ggcagtcttt
    25861 gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa atggagtgag
    25921 gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt cactttgact
    25981 ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta ttattggcaa
    26041 gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt atccatcaaa
    26101 aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc aaaagaacaa
    26161 gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg cactaccgaa
    26221 actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa tgaaatcaca
    26281 ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga tggttatggc
    26341 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc cccagaaggt
    26401 tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa agaagacttt
    26461 gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc aattaaaacg
    26521 gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt gaaccgttta
    26581 atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac ttggaaagac
    26641 ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt gagttacaat
    26701 ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggatgcgaca
    26761 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga aacaagcgca
    26821 acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt aaccgccgaa
    26881 caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa ggaagctttt
    26941 gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc ccaaactgtc
    27001 agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga aaacggttct
    27061 cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat tactcatgat
    27121 gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt accggatggt
    27181 acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga taaagaattc
    27241 accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg aaccaagttt
    27301 acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca caatgaagat
    27361 ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact aagtacgccg
    27421 aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc cacagtgaag
    27481 accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg ttttactttg
    27541 atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga tgagatgata
    27601 caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt cgcgctattt
    27661 ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat ttgtcgtgcc
    27721 agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa ttataacaga
    27781 aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt ccagtaagca
    27841 acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg attttgatca
    27901 agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa
    27961 tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat aggaggaaaa
    28021 gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg ggaagttgcg
    28081 tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa ttggtaatga
    28141 ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga ttcaagttag
    28201 tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag agttggtcaa
    28261 tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg ttaattggtt
    28321 cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc ctacgaacca
    28381 aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata ttttagattg
    28441 tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag cattaacctc
    28501 tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata gcgacagtga
    28561 agaagactct gatgtactag actggttagg agaagaagag tctttcttta tagttgtcga
    28621 agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg gtgtggagat
    28681 gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac tcccctcctc
    28741 gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct attgggcttt
    28801 ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc
    28861 agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga
    28921 gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca ctttctacta
    28981 ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc gctttttcag
    29041 gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc ttgtggcatc
    29101 gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa caaactcaaa
    29161 gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc agttacttcc
    29221 ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa atttcacttg
    29281 ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga ctgtattgtg
    29341 agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg tatgatatga
    29401 tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg cgattgatga
    29461 aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt ggcacgggtg
    29521 gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca gatgcagaat
    29581 tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg atgcctcatg
    29641 tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa cgtatgatgg
    29701 ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat tatgcctatc
    29761 ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg gagatgttaa
    29821 cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttgggtcgtc
    29881 aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat ttaggagacg
    29941 gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa ctcggatatt
    30001 ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa ggtcgaggtt
    30061 atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta ccaaaagggt
    30121 atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg atcttcaata
    30181 ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa aggggataat
    30241 taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat
    30301 ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg
    30361 gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca
    30421 tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaactc
    30481 tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat atgtcttaga
    30541 tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat
    30601 ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc aagaccctaa
    30661 aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa
    30721 ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca
    30781 tccagatgta ttgcttcata tcataataaa agattatacg aataatattt ccgaagttca
    30841 ttatgctacg gataatctag aattattttt accactaggg tatagtagga aagatttgga
    30901 ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa tacaataaga
    30961 gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg attaaatatg
    31021 tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa aaatagtagg
    31081 acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct ttgcaatgaa
    31141 tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt cgtttaggta
    31201 tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt gctagacgta
    31261 gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc acaagtgtat
    31321 cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga tttccgtgcc
    31381 aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt actttattct
    31441 ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt gagaacgtcc
    31501 gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga acgctggatg
    31561 gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt ccccaaagcc
    31621 gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa gtatttccta
    31681 tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat atcaatcctt
    31741 ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca accttaacag
    31801 gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta gcaggactat
    31861 taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc gcccccacct
    31921 tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca gaatatcaag
    31981 aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt cgccgaggtc
    32041 gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg atcgtcacgg
    32101 atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag ggctttcccg
    32161 attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat aagcaagccg
    32221 gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa gagttggatg
    32281 tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa gattcagtca
    32341 gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa gaatggtgtc
    32401 aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat tgaaataaat
    32461 caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga tattcgtcaa
    32521 tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga gtaacctaaa
    32581 ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga aatcctccgt
    32641 ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc ccgtatctaa
    32701 caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct tgttgtatca
    32761 aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca ccttgaattt
    32821 ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga ccgacgcaac
    32881 gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg gccgtactta
    32941 aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa aatttttgac
    33001 caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta tgtaaggatt
    33061 cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt gaaatttgag
    33121 tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt catgggtgat
    33181 attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt aaaggggcga
    33241 ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta cgactttttc
    33301 gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat caatgatcga
    33361 gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga atgtatctcc
    33421 ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga tgaaaaggac
    33481 ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt ctgtttgtat
    33541 gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac tgagacgaaa
    33601 aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca agatttgtta
    33661 aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt gcgatttgcg
    33721 gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg ggggcgtttt
    33781 attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta taccattgaa
    33841 cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc taaggagttg
    33901 gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg cttatcagac
    33961 cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat tatatgaaaa
    34021 gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc agtatgcttg
    34081 gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca atggaaaaga
    34141 aaggaaagtg acaagatgaa tttcggacaa aattt
//
LOCUS       DAZBTH010000020        30392 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000020 DAZBTH010000000
VERSION     DAZBTH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.020"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..229)
                     /locus_tag="ACHGNW_000940"
     CDS             complement(32..229)
                     /locus_tag="ACHGNW_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6925273.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(623..2350)
                     /locus_tag="ACHGNW_000941"
     CDS             complement(623..2350)
                     /locus_tag="ACHGNW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN6925274.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2343..3536)
                     /locus_tag="ACHGNW_000942"
     CDS             complement(2343..3536)
                     /locus_tag="ACHGNW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6925275.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3723..4367)
                     /locus_tag="ACHGNW_000943"
     CDS             complement(3723..4367)
                     /locus_tag="ACHGNW_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6925276.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4461..4595)
                     /locus_tag="ACHGNW_000944"
     CDS             complement(4461..4595)
                     /locus_tag="ACHGNW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN6925277.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4597..6729)
                     /gene="feoB"
                     /locus_tag="ACHGNW_000945"
     CDS             complement(4597..6729)
                     /gene="feoB"
                     /locus_tag="ACHGNW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN6925278.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6726..7199)
                     /locus_tag="ACHGNW_000946"
     CDS             complement(6726..7199)
                     /locus_tag="ACHGNW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN6925279.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7600..7824
                     /gene="nrdH"
                     /locus_tag="ACHGNW_000947"
     CDS             7600..7824
                     /gene="nrdH"
                     /locus_tag="ACHGNW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN6925280.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7983..10142
                     /gene="nrdE"
                     /locus_tag="ACHGNW_000948"
     CDS             7983..10142
                     /gene="nrdE"
                     /locus_tag="ACHGNW_000948"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN6925281.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10192..11157
                     /gene="nrdF"
                     /locus_tag="ACHGNW_000949"
     CDS             10192..11157
                     /gene="nrdF"
                     /locus_tag="ACHGNW_000949"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN6925282.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11246..12385)
                     /locus_tag="ACHGNW_000950"
     CDS             complement(11246..12385)
                     /locus_tag="ACHGNW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6925283.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12694..13407)
                     /locus_tag="ACHGNW_000951"
     CDS             complement(12694..13407)
                     /locus_tag="ACHGNW_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN6925284.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13661..14362)
                     /gene="nagB"
                     /locus_tag="ACHGNW_000952"
     CDS             complement(13661..14362)
                     /gene="nagB"
                     /locus_tag="ACHGNW_000952"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN6925285.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14596..16128
                     /locus_tag="ACHGNW_000953"
     CDS             14596..16128
                     /locus_tag="ACHGNW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6925286.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16319..17521)
                     /locus_tag="ACHGNW_000954"
     CDS             complement(16319..17521)
                     /locus_tag="ACHGNW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925287.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17603..18292)
                     /locus_tag="ACHGNW_000955"
     CDS             complement(17603..18292)
                     /locus_tag="ACHGNW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6925288.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18398..18766)
                     /gene="rplL"
                     /locus_tag="ACHGNW_000956"
     CDS             complement(18398..18766)
                     /gene="rplL"
                     /locus_tag="ACHGNW_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN6925289.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18819..19322)
                     /gene="rplJ"
                     /locus_tag="ACHGNW_000957"
     CDS             complement(18819..19322)
                     /gene="rplJ"
                     /locus_tag="ACHGNW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN6925290.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19526..19653)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19732..20421)
                     /gene="rplA"
                     /locus_tag="ACHGNW_000958"
     CDS             complement(19732..20421)
                     /gene="rplA"
                     /locus_tag="ACHGNW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN6925291.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20525..20947)
                     /gene="rplK"
                     /locus_tag="ACHGNW_000959"
     CDS             complement(20525..20947)
                     /gene="rplK"
                     /locus_tag="ACHGNW_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN6925292.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21290..21958
                     /gene="sdaAB"
                     /locus_tag="ACHGNW_000960"
     CDS             21290..21958
                     /gene="sdaAB"
                     /locus_tag="ACHGNW_000960"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN6925293.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21971..22843
                     /gene="sdaAA"
                     /locus_tag="ACHGNW_000961"
     CDS             21971..22843
                     /gene="sdaAA"
                     /locus_tag="ACHGNW_000961"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN6925294.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22934..24034)
                     /locus_tag="ACHGNW_000962"
                     /pseudo
     CDS             complement(22934..24034)
                     /locus_tag="ACHGNW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24126..25013)
                     /locus_tag="ACHGNW_000963"
     CDS             complement(24126..25013)
                     /locus_tag="ACHGNW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6925295.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25090..25635)
                     /gene="nusG"
                     /locus_tag="ACHGNW_000964"
     CDS             complement(25090..25635)
                     /gene="nusG"
                     /locus_tag="ACHGNW_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN6925296.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25736..25906)
                     /gene="secE"
                     /locus_tag="ACHGNW_000965"
     CDS             complement(25736..25906)
                     /gene="secE"
                     /locus_tag="ACHGNW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN6925297.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25930..26082)
                     /gene="rpmG"
                     /locus_tag="ACHGNW_000966"
     CDS             complement(25930..26082)
                     /gene="rpmG"
                     /locus_tag="ACHGNW_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6925298.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26244..26888
                     /gene="cbpA"
                     /locus_tag="ACHGNW_000967"
     CDS             26244..26888
                     /gene="cbpA"
                     /locus_tag="ACHGNW_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN6925299.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26944..27447)
                     /locus_tag="ACHGNW_000968"
     CDS             complement(26944..27447)
                     /locus_tag="ACHGNW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6925300.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27460..27506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28044..29009
                     /locus_tag="ACHGNW_000969"
     CDS             28044..29009
                     /locus_tag="ACHGNW_000969"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6925301.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29108..30280)
                     /locus_tag="ACHGNW_000970"
     CDS             complement(29108..30280)
                     /locus_tag="ACHGNW_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925302.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30267..>30392)
                     /locus_tag="ACHGNW_000971"
     CDS             complement(30267..>30392)
                     /locus_tag="ACHGNW_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925303.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9266 a         6252 c         5394 g         9480 t
ORIGIN      
        1 atgaacaatt tgcttagcat tttttttcat tttagcactt ccctggaatt gctccaccta
       61 aaaaaccacc tatagacatt cctgcaatac aagtagttgc cttggcccaa tcgaccgtac
      121 atttattttt agtgcaatac actccatttc cataatattt tccactatga gtggtacccc
      181 catagataag ttgtgtctct ttaatagaca aaatttttaa atgtttcata atctaatcac
      241 ccctaaatta atgtttttag actattattc taacattcat taaatttcaa aaaacaattc
      301 cttcttgaaa cgtagcaaaa taattcctga ataagaattt aatcactttt aagtatataa
      361 actatgtata ttctttaata ttttttgaga atgcacttcg tacataataa actttaatca
      421 cattagtctt tcatacctac gatcgatctc tttgactcgc acccataaat tcataatagt
      481 ttgtcggcgt ctttggacgt ttcgaagatg agctacgcat tctctttttt tatagacaaa
      541 aaagatagag acgaacatgg agcttccaaa tttggatatt tgatccaatt ttggaagctc
      601 catttatttc gtgtctgtct ttttattttt tccagctttc gactaattct tggttctctt
      661 tgatccagtc ttttgctgct tgctgtggat cttttccatt gttgatttcc aacataactt
      721 tttccatatc tttttctgac caatggaaat tgtctaatac tttgtaagct tctggctggt
      781 cttctttcaa tccttttcga accatcgtat ggatctgctc ttcgcttccc attccgtttt
      841 caggatcttc taagtatttc agatcatatt tggcaaacat ccaatgaggt gtccatcctg
      901 taactacaat tggttcgtgt tttttgatcg cttgacctag tgcaacggtc attgctcctg
      961 aagaagatgt ctctactttc cagtctttca agttgtcata tttttggatc gtgttttcag
     1021 cagccgtaac tacaccagct cctggctcga ttcctatgat tttcttacct gcctgatcgg
     1081 ttaactcatc gatcgaattt acatccatgt aggctggcac tgctaaacca acttttgctc
     1141 ctgtcaaatt ggcacctagg tcttcgactt gatctttata ttgtgcatat tgtgaaccat
     1201 gagttttagg tagccaagct gaaaccatgg catctgactc gccatttgaa acagatttcc
     1261 acatgattga attatctaat ggcgtcatcg ttacatcata gcccatttgt ttcaatactt
     1321 caccgactac atttgttgaa gcaacttctg tatcccattc aacataagat aagttgatac
     1381 gttttgtttc tttggcagat gaaaaactcc ctataccaat cgcacctaag atcactacta
     1441 ctacagcacc aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag
     1501 cttttttatt gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga
     1561 taactaatgc aaccccgttg acaaatccat tcccgacttg tgctctttgc aatgctgata
     1621 atacgccgcg tcctaagccg ggtgcaccga tcatagaggc aataacgacc attgaaagtg
     1681 acagcattgt tgtttgattg atccctgcca tgatcgtact tttagctaaa ggcaattcta
     1741 gtttgaatag tttttgacga cttgtgctcc cgaatgaatc agaagcttct actaattctt
     1801 taggaacttg tcgaatccct aaattcgtaa aacgaacggt tggtggcaga gcaaagatga
     1861 ctgaggcaaa tacccctgga accatcccga taccgaaaaa tgcaactgct ggaatcaaat
     1921 aaacgaatcc tggcattgtc tgcataaagt caaggatcgg tttgataata ctttgggctt
     1981 tactgctttt tgccatcaag atccctaatg gtaccccaat gatgatcgag atcacactag
     2041 ataaaagaac aagtgtgaca gtattcatca aatcattcca tagaccttgg ttataaataa
     2101 accatagacc gattaatgtg aacaacgtaa gtcctggacg tttcttagaa ataaagaaag
     2161 ctgctaccgt caataacaag ataaacaaag gagctggaat cactaacagt aaatttgtaa
     2221 tccccgacat cactgcctgt ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg
     2281 tcgtaaacca ttctgtgata ttttctaccc agtttgccac tggtaattga tagttcaata
     2341 gattattcat gttcactcac ctcgcttgtt tctgctaatg cttcaatcac actacctcgg
     2401 atgacgacac cttttaattt tccatcctct acaactgcaa gtggagtcgg tgagtcataa
     2461 ataatattga aaatatcact taccaccatg tctttcccaa tcaccgtcac attttcatct
     2521 aggaagtcga tcaatggacg tttttgcttc ctagcttcta acgcttgatc tgcagtgatg
     2581 atccctttca ggtgtctctt tctgtcaaca gccatcagca tgctgacttc ttcattacgc
     2641 atccttgtca atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc
     2701 atgatgtttt gagccgtaag cactttggaa cgatcgactt cttccacaaa ttcacgtaca
     2761 taatcatttg ctggattggt caatatttct tcacctgttc cgatctgcat gatttcgccg
     2821 tctttcatca aggcgattct gtccccaatc cgtaaagctt cattcaaatc atgggtaatg
     2881 aaaataatcg ttttttgaac attggcttgc aggtccagta attcgtcttg catttctcgc
     2941 cggatcagcg gatcaagagc cgagaacgct tcgtccatca gcaagatctc aggatcattt
     3001 gctaaagctc tggctaggcc aacccgttgt tgcatcccac cagataactg actaggatat
     3061 tggtctttaa atgaaagcaa actagaattt tctaatgctt tttctgcttt ttcttgtcgt
     3121 tcttctttag gtacaccacg gacttctaac ccgtattctg tattttccaa gatcgttcga
     3181 tgtggaaaca gaccgaagtt ttggaaaacc atattgattt tatgacgacg gacttctcgc
     3241 aattcttctt tgcttaactt cgatacatct tctccatcaa tatagatact gcccgaagtt
     3301 ggctcaatca gacgattcaa tagacggatc aatgtggatt tcccactccc ggaaagcccc
     3361 atgatcacga agatttctcc ttcttttacg tcaaaattca cgtcatacac accaaccgtt
     3421 gcaccggttt tctttaaaat ctccgttttc ggctggtgat cattcatcat atctaaagcg
     3481 gcttgtgtct ttttaccaaa tatctttgtt aaatgtttta cttctacttt taccaaacaa
     3541 attctccttt tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac actatgttaa
     3601 catcatgtag tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact
     3661 tggctaaatc tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac
     3721 aatcattctg caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat
     3781 tcatttccaa taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa
     3841 acaagcagtt cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata
     3901 ttcaactcgc taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac
     3961 ggaagtaaag gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat
     4021 tgagaaaaat gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt
     4081 gtatcttgga tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc
     4141 gaagcaacaa atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca
     4201 ttgatagcct tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg
     4261 gcatggatct tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca
     4321 gcaatctgct ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt
     4381 tcttttttta ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa
     4441 tagcctcttg caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt
     4501 tgacagtcat cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt
     4561 ccaaagatta acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa
     4621 tatagtataa aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat
     4681 catcagtaaa atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc
     4741 caactgataa atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat
     4801 ccaagtccat ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg
     4861 cgcacaaagt aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg
     4921 tagcacttgc cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc
     4981 aaatgtgcca ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg
     5041 ccagtttccc caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa
     5101 gatactttgg tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat
     5161 cacgatacta gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc
     5221 ataggtttgt cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat
     5281 aaaaggagct gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat
     5341 aattgcagtc ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa
     5401 ggcccctgct actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac
     5461 catgatcgtc aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg
     5521 cactccacat cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt
     5581 cctgaaaagt cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa
     5641 gctcaagcat aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc
     5701 tgcgatgatg ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa
     5761 caatctgcca acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt
     5821 ccccattgtt cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa
     5881 gatcggcaag gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa
     5941 ttggaaagaa tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg
     6001 ttcattgacc agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag
     6061 ttgctcgatc tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc
     6121 tcgttcaaaa tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc
     6181 ttcaatttca tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga
     6241 aggccgcttt tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt
     6301 caatacgctg attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat
     6361 tttttttcct tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc
     6421 taaatccaat aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat
     6481 gttgatgatc agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg
     6541 tgtataagga gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt
     6601 tttcaacgat ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt
     6661 cgaaccagtc aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc
     6721 gatattcatt ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac
     6781 gtcagttcat accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg
     6841 ataactattt ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct
     6901 tgtccgtgga ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc
     6961 cagctttcag aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt
     7021 actgccactc gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg
     7081 acgattttac ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct
     7141 gtttcgattc gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata
     7201 ttgattcctc tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt
     7261 tcttacgtgt tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga
     7321 gaatgtcaat tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga
     7381 gaaaaaaata tttcttcata gttttccaca attttttctc tattaaattt gctattttcc
     7441 cacacgatac ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt
     7501 ccacaacaca atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct
     7561 attatagata ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct
     7621 aaaaataatt gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct
     7681 tttgaagaag tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga
     7741 ttccaaagtg ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat
     7801 cagttgcgta aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt
     7861 gaagggcttg agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta
     7921 aaaacaatgc aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac
     7981 gtatgagtct taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc
     8041 ggccagtcaa tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg
     8101 aggaaaatgt caaaccaaat acgatgactt ttctatctat tatggaaaaa atcaattact
     8161 tgatcgatca agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg
     8221 aaaagctata tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt
     8281 acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt
     8341 atgaagatcg agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac
     8401 ttgctcttgc agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga
     8461 atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg
     8521 atatgaactc tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg
     8581 gcgtagggat tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg
     8641 gtgctgcaag tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta
     8701 atcaattagg tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca
     8761 ttattgcttt cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc
     8821 tttctcttgg cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata
     8881 tgtatttatt tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa
     8941 atattacagc tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca
     9001 aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg
     9061 taatcaatat tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta
     9121 atttatgttc agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat
     9181 atgaagtatt aggaacggat atctcatgta acttaggctc gacaaatatc gtgaatttga
     9241 tgcaaagccc taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta
     9301 cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata
     9361 cgattggatt aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg
     9421 gctcagagga ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac
     9481 ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat
     9541 cagcttatgc tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat
     9601 cagaaaaagt caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg
     9661 ctttgaaaca agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac
     9721 caaatggctc aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca
     9781 tgatcgaaga acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat
     9841 ccaatgagac gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg
     9901 atgtttatgc aatggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc
     9961 gttctgaaat cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg
    10021 atttgaatat tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac
    10081 gtacctttac tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct
    10141 aatatcatag atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa
    10201 acatattatg aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg
    10261 gagaagctga cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat
    10321 gattggcgta cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg
    10381 acactgctag atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc
    10441 actccccatg aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa
    10501 agttattctt ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac
    10561 tggacaaata cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa
    10621 cacggtacac cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat
    10681 tcaggttttt atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag
    10741 atcatcaaac taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc
    10801 caattaggat tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac
    10861 aatcttcttt atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca
    10921 atcggctgga cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg
    10981 aacctaggga tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg
    11041 aacgggattt caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat
    11101 ttgttaggtc atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga
    11161 tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa
    11221 atgtacactg aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt
    11281 ctaacttttc cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata
    11341 ttatcgtctt aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga
    11401 cacttcctgc tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc
    11461 caaataacaa cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg
    11521 ccatatacaa agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc
    11581 caataaaagg gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca
    11641 agctataagg ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag
    11701 catctaagat cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt
    11761 ggcgtaactc tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt
    11821 gcccatcctt gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt
    11881 gcatcataaa tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga
    11941 acaaattact aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa
    12001 actcttcccg ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca
    12061 agctctgggt caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta
    12121 aaacaactaa aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc
    12181 gatgcttttc gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa
    12241 gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt
    12301 gtaaaataaa gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt
    12361 ttgattgttt aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct
    12421 atcatttaac aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat
    12481 aaacatattt taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa
    12541 aaattatact gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa
    12601 taagcccagc caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg
    12661 atgtaggaca tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa
    12721 ctttgtgata tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt
    12781 tggaagatca atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc
    12841 aaaattcaca tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt
    12901 ggtttctttt ctatggataa attggatttc taacttttta gtcgtaggta catggatcac
    12961 gcgtttcact actgtgagtg caatagcaat cccgataaaa ctgatgatcg caatggaata
    13021 gtaacccatg ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt
    13081 aagtcccgtc accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc
    13141 actcaccact tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg
    13201 atactctaaa gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat
    13261 acatgctccc atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg
    13321 tcgttcaaaa ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc
    13381 tggaattgtc aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg
    13441 aaaggagcag acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa
    13501 tttttgtcat agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac
    13561 tcaacaaacg gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca
    13621 aaacgctgat ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt
    13681 cttcatcgat gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat
    13741 tcgttacagg tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca
    13801 tcaagacgat ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg
    13861 gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt
    13921 caactactga agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat
    13981 tttgcccgat tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact
    14041 ttttgcatgc tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg
    14101 gttttttatc aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc
    14161 ctagtccaac gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg
    14221 aagtcatttc tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg
    14281 cacctttgtt cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt
    14341 tttttacacg aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat
    14401 agcattcttt ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt
    14461 cttgacaata aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat
    14521 gttgcatgaa aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat
    14581 gaaaaaggag ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac
    14641 aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt
    14701 ggacaaaacg gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca
    14761 gatgacggac agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg
    14821 gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac
    14881 gaaaaggaag aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc
    14941 ttgctggaaa aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc
    15001 gacaccttag ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca
    15061 ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta
    15121 gaaaacccag atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc
    15181 caatggctga cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac
    15241 caagaatttc ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact
    15301 aagtatactg gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat
    15361 ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa
    15421 tacaaagccg gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc
    15481 gaacggctga cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct
    15541 attgtcacga cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta
    15601 cttgcaccca tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac
    15661 gggattggaa aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc
    15721 gatttccact tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa
    15781 aatccattag aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa
    15841 gaattacgtc gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc
    15901 actacattaa gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa
    15961 ggtaatttac tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc
    16021 ttacaagacg ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa
    16081 ttttatcaag atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga
    16141 atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat
    16201 ggtggccaat tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat
    16261 ttttatttgg ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct
    16321 attttttatt ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac
    16381 atccttgaaa aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg
    16441 tcgtccctag caatgaacca gccgcacctc caataaagta cataaacata aatacagtat
    16501 tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac
    16561 caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc
    16621 cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt
    16681 ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca
    16741 aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga
    16801 tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga
    16861 ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat
    16921 caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca
    16981 gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa
    17041 gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa
    17101 gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa
    17161 tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg
    17221 taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct gccattcgaa
    17281 tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg
    17341 catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac
    17401 tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac
    17461 aagtgattga taatagtaaa ataagcgaag ttgttaaagg ttttttttca ttactcttca
    17521 ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa
    17581 atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat
    17641 ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg
    17701 cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag
    17761 ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg
    17821 cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta
    17881 tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat
    17941 ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca
    18001 aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta
    18061 ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg
    18121 atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat
    18181 ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta
    18241 tcagaatgat tcccaacgag atcaatatca atcggattat tttctttttc atatttcctc
    18301 ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa
    18361 tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc
    18421 caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa
    18481 ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt
    18541 cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga
    18601 attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag
    18661 cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag
    18721 ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg
    18781 tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct
    18841 ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat
    18901 agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc
    18961 aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta
    19021 gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct
    19081 gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa
    19141 agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa
    19201 cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct
    19261 gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc
    19321 atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt
    19381 cacttttata gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag
    19441 tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc
    19501 atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca
    19561 tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc
    19621 acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag
    19681 ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga
    19741 agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta
    19801 ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt
    19861 ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc
    19921 tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc
    19981 cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc
    20041 tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca
    20101 tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc
    20161 agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc
    20221 gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa
    20281 acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc
    20341 tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat
    20401 ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct
    20461 cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta
    20521 tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg
    20581 cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat
    20641 cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag
    20701 ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt
    20761 cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt
    20821 tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg
    20881 gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct
    20941 ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc
    21001 ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac
    21061 tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt
    21121 tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc
    21181 ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt
    21241 tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt
    21301 aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca
    21361 cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga
    21421 ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga
    21481 tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc
    21541 tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc
    21601 cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac
    21661 tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc
    21721 tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc
    21781 taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg
    21841 ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga
    21901 aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg
    21961 agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag
    22021 tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat
    22081 catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga
    22141 aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt
    22201 aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc
    22261 tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc
    22321 gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca
    22381 gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc
    22441 tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc
    22501 agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat
    22561 tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc
    22621 gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt
    22681 agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg
    22741 acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac
    22801 tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc
    22861 gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc
    22921 tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc
    22981 ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc
    23041 cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag
    23101 caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca
    23161 ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac
    23221 ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga
    23281 tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga
    23341 tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt
    23401 ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt
    23461 taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga
    23521 gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa
    23581 tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg
    23641 gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg
    23701 atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt
    23761 gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat
    23821 gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag
    23881 aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag
    23941 ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga
    24001 ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga
    24061 ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt
    24121 tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga
    24181 agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt
    24241 tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag
    24301 tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg
    24361 atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca
    24421 tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt
    24481 aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca
    24541 ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc
    24601 agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct
    24661 tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt
    24721 atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc
    24781 aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt
    24841 gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa
    24901 acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg
    24961 actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc
    25021 ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa
    25081 gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg
    25141 tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg
    25201 tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc
    25261 tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg
    25321 aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg
    25381 tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc
    25441 tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc
    25501 ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc
    25561 gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt
    25621 ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac
    25681 ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt
    25741 ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga
    25801 aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc
    25861 aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct
    25921 tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt
    25981 attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga
    26041 cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc
    26101 tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga
    26161 aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa
    26221 atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc
    26281 tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg
    26341 gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca
    26401 aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc
    26461 ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta
    26521 cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt
    26581 tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct
    26641 atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca
    26701 tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca
    26761 ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg
    26821 tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag
    26881 aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta
    26941 taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga
    27001 aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg
    27061 gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt
    27121 gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc
    27181 taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa
    27241 ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac
    27301 taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac
    27361 taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa
    27421 ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg
    27481 cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat
    27541 ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt
    27601 gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa
    27661 agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag
    27721 gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta
    27781 agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta
    27841 ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat
    27901 aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa
    27961 atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt
    28021 ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg
    28081 caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc
    28141 atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa
    28201 ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt
    28261 tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct
    28321 atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga gatgattttg
    28381 ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc
    28441 aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa
    28501 tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt
    28561 cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta
    28621 ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac
    28681 aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag
    28741 gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg
    28801 aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat
    28861 tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac
    28921 cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa
    28981 ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg
    29041 aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac
    29101 tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa
    29161 taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa
    29221 aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa
    29281 caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg
    29341 ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac
    29401 cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca
    29461 ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga
    29521 tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca
    29581 aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt
    29641 tactgatctc tttgcgatta aatgctagat tgatgacgct gaaaccgcta aaaatggata
    29701 gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct
    29761 gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt
    29821 ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg
    29881 tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga
    29941 tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa
    30001 tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg
    30061 taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt
    30121 cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt
    30181 gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat
    30241 ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc
    30301 caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa
    30361 aaactagcca aatcagtgta gaaataaagt tt
//
LOCUS       DAZBTH010000021        29099 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000021 DAZBTH010000000
VERSION     DAZBTH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.021"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACHGNW_000972"
     CDS             complement(<1..209)
                     /locus_tag="ACHGNW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925304.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGK"
     gene            complement(229..651)
                     /locus_tag="ACHGNW_000973"
     CDS             complement(229..651)
                     /locus_tag="ACHGNW_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6925305.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(795..1505)
                     /locus_tag="ACHGNW_000974"
     CDS             complement(795..1505)
                     /locus_tag="ACHGNW_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN6925306.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1523..2020)
                     /locus_tag="ACHGNW_000975"
     CDS             complement(1523..2020)
                     /locus_tag="ACHGNW_000975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925307.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2057..2509)
                     /locus_tag="ACHGNW_000976"
     CDS             complement(2057..2509)
                     /locus_tag="ACHGNW_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6925308.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2644..3249)
                     /locus_tag="ACHGNW_000977"
     CDS             complement(2644..3249)
                     /locus_tag="ACHGNW_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6925309.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3699..4319)
                     /locus_tag="ACHGNW_000978"
     CDS             complement(3699..4319)
                     /locus_tag="ACHGNW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6925310.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4321..5190)
                     /locus_tag="ACHGNW_000979"
     CDS             complement(4321..5190)
                     /locus_tag="ACHGNW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6925311.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5213..5620)
                     /locus_tag="ACHGNW_000980"
     CDS             complement(5213..5620)
                     /locus_tag="ACHGNW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6925312.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5639..6928)
                     /locus_tag="ACHGNW_000981"
     CDS             complement(5639..6928)
                     /locus_tag="ACHGNW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6925313.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6955..7257)
                     /locus_tag="ACHGNW_000982"
     CDS             complement(6955..7257)
                     /locus_tag="ACHGNW_000982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6925314.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7282..9375)
                     /locus_tag="ACHGNW_000983"
     CDS             complement(7282..9375)
                     /locus_tag="ACHGNW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6925315.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9864..10628)
                     /locus_tag="ACHGNW_000984"
     CDS             complement(9864..10628)
                     /locus_tag="ACHGNW_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN6925316.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10739..11608)
                     /gene="mdcB"
                     /locus_tag="ACHGNW_000985"
     CDS             complement(10739..11608)
                     /gene="mdcB"
                     /locus_tag="ACHGNW_000985"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN6925317.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(11633..12709)
                     /locus_tag="ACHGNW_000986"
     CDS             complement(11633..12709)
                     /locus_tag="ACHGNW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6925318.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(12733..13326)
                     /locus_tag="ACHGNW_000987"
     CDS             complement(12733..13326)
                     /locus_tag="ACHGNW_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN6925319.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13330..15006)
                     /gene="mdcD"
                     /locus_tag="ACHGNW_000988"
     CDS             complement(13330..15006)
                     /gene="mdcD"
                     /locus_tag="ACHGNW_000988"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN6925320.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14996..15295)
                     /locus_tag="ACHGNW_000989"
     CDS             complement(14996..15295)
                     /locus_tag="ACHGNW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN6925321.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15397..17043)
                     /gene="mdcA"
                     /locus_tag="ACHGNW_000990"
     CDS             complement(15397..17043)
                     /gene="mdcA"
                     /locus_tag="ACHGNW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFN6925322.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17050..17961)
                     /locus_tag="ACHGNW_000991"
     CDS             complement(17050..17961)
                     /locus_tag="ACHGNW_000991"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6925323.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18081..18938
                     /locus_tag="ACHGNW_000992"
     CDS             18081..18938
                     /locus_tag="ACHGNW_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6925324.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(19125..19487)
                     /locus_tag="ACHGNW_000993"
     CDS             complement(19125..19487)
                     /locus_tag="ACHGNW_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN6925325.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(19676..19858)
                     /locus_tag="ACHGNW_000994"
     CDS             complement(19676..19858)
                     /locus_tag="ACHGNW_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925326.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            19833..21272
                     /locus_tag="ACHGNW_000995"
     CDS             19833..21272
                     /locus_tag="ACHGNW_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925327.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(21279..23831)
                     /locus_tag="ACHGNW_000996"
     CDS             complement(21279..23831)
                     /locus_tag="ACHGNW_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN6925328.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(23945..25276)
                     /locus_tag="ACHGNW_000997"
     CDS             complement(23945..25276)
                     /locus_tag="ACHGNW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6925329.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(25242..26720)
                     /locus_tag="ACHGNW_000998"
     CDS             complement(25242..26720)
                     /locus_tag="ACHGNW_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6925330.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(27025..28860)
                     /gene="tdc"
                     /locus_tag="ACHGNW_000999"
     CDS             complement(27025..28860)
                     /gene="tdc"
                     /locus_tag="ACHGNW_000999"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6925331.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         8358 a         6191 c         4952 g         9598 t
ORIGIN      
        1 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
       61 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
      121 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
      181 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
      241 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
      301 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
      361 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
      421 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
      481 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
      541 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
      601 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
      661 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
      721 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
      781 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
      841 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
      901 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
      961 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
     1021 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
     1081 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
     1141 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
     1201 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
     1261 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
     1321 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
     1381 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
     1441 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
     1501 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
     1561 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
     1621 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
     1681 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
     1741 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
     1801 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
     1861 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
     1921 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
     1981 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
     2041 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
     2101 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
     2161 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
     2221 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
     2281 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
     2341 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
     2401 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
     2461 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
     2521 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
     2581 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
     2641 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
     2701 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
     2761 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
     2821 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
     2881 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
     2941 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
     3001 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
     3061 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
     3121 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
     3181 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
     3241 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
     3301 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
     3361 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
     3421 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
     3481 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
     3541 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
     3601 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
     3661 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
     3721 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
     3781 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
     3841 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
     3901 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
     3961 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
     4021 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
     4081 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
     4141 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
     4201 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
     4261 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
     4321 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
     4381 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
     4441 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
     4501 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
     4561 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
     4621 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
     4681 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
     4741 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
     4801 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
     4861 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
     4921 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
     4981 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
     5041 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
     5101 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
     5161 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
     5221 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
     5281 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
     5341 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
     5401 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
     5461 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
     5521 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
     5581 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
     5641 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
     5701 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
     5761 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
     5821 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
     5881 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
     5941 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
     6001 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
     6061 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
     6121 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
     6181 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
     6241 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
     6301 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
     6361 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
     6421 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
     6481 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
     6541 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
     6601 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
     6661 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
     6721 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
     6781 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
     6841 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
     6901 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
     6961 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
     7021 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
     7081 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
     7141 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
     7201 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
     7261 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
     7321 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
     7381 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
     7441 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
     7501 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
     7561 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
     7621 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
     7681 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
     7741 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
     7801 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
     7861 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
     7921 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
     7981 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
     8041 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
     8101 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
     8161 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
     8221 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
     8281 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
     8341 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
     8401 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
     8461 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
     8521 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
     8581 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
     8641 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
     8701 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
     8761 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
     8821 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
     8881 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
     8941 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
     9001 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
     9061 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
     9121 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
     9181 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
     9241 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
     9301 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
     9361 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
     9421 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
     9481 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
     9541 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
     9601 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
     9661 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
     9721 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
     9781 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
     9841 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
     9901 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
     9961 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
    10021 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
    10081 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
    10141 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
    10201 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
    10261 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
    10321 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
    10381 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
    10441 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
    10501 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
    10561 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
    10621 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
    10681 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
    10741 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
    10801 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
    10861 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
    10921 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
    10981 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
    11041 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
    11101 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
    11161 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
    11221 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
    11281 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
    11341 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
    11401 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
    11461 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
    11521 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
    11581 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
    11641 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
    11701 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
    11761 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
    11821 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
    11881 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
    11941 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
    12001 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
    12061 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
    12121 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
    12181 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
    12241 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
    12301 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
    12361 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
    12421 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
    12481 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
    12541 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
    12601 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
    12661 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
    12721 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
    12781 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
    12841 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
    12901 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
    12961 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
    13021 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
    13081 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
    13141 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
    13201 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
    13261 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
    13321 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
    13381 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
    13441 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
    13501 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
    13561 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
    13621 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
    13681 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
    13741 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
    13801 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
    13861 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
    13921 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
    13981 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
    14041 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
    14101 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
    14161 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
    14221 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
    14281 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
    14341 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
    14401 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
    14461 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
    14521 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
    14581 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
    14641 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
    14701 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
    14761 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
    14821 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
    14881 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
    14941 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
    15001 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
    15061 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
    15121 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
    15181 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
    15241 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
    15301 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
    15361 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
    15421 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
    15481 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
    15541 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
    15601 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
    15661 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
    15721 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
    15781 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
    15841 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
    15901 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
    15961 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
    16021 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
    16081 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
    16141 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
    16201 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
    16261 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
    16321 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
    16381 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
    16441 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
    16501 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
    16561 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
    16621 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
    16681 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
    16741 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
    16801 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
    16861 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
    16921 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
    16981 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
    17041 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
    17101 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
    17161 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
    17221 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
    17281 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
    17341 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
    17401 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
    17461 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
    17521 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
    17581 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
    17641 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
    17701 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
    17761 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
    17821 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
    17881 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
    17941 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
    18001 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
    18061 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
    18121 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
    18181 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
    18241 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
    18301 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
    18361 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
    18421 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
    18481 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
    18541 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
    18601 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
    18661 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
    18721 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
    18781 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
    18841 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
    18901 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
    18961 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
    19021 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    19081 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
    19141 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
    19201 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
    19261 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
    19321 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
    19381 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
    19441 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
    19501 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
    19561 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
    19621 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
    19681 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
    19741 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
    19801 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
    19861 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
    19921 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
    19981 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
    20041 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
    20101 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
    20161 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
    20221 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
    20281 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
    20341 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
    20401 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
    20461 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
    20521 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
    20581 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
    20641 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
    20701 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
    20761 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
    20821 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
    20881 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
    20941 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
    21001 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
    21061 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
    21121 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
    21181 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
    21241 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
    21301 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
    21361 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
    21421 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
    21481 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
    21541 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
    21601 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
    21661 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
    21721 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
    21781 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
    21841 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
    21901 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
    21961 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
    22021 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
    22081 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
    22141 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
    22201 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
    22261 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
    22321 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
    22381 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
    22441 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
    22501 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
    22561 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
    22621 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
    22681 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
    22741 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
    22801 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
    22861 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
    22921 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
    22981 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
    23041 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
    23101 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
    23161 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
    23221 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
    23281 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
    23341 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
    23401 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
    23461 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
    23521 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
    23581 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
    23641 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
    23701 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
    23761 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
    23821 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
    23881 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
    23941 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
    24001 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
    24061 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
    24121 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
    24181 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
    24241 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
    24301 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
    24361 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
    24421 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
    24481 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
    24541 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
    24601 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
    24661 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
    24721 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
    24781 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
    24841 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
    24901 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
    24961 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
    25021 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
    25081 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
    25141 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
    25201 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
    25261 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
    25321 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
    25381 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
    25441 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
    25501 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
    25561 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
    25621 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
    25681 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
    25741 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
    25801 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
    25861 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
    25921 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
    25981 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
    26041 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
    26101 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
    26161 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
    26221 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
    26281 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
    26341 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
    26401 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
    26461 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
    26521 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
    26581 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
    26641 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
    26701 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
    26761 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
    26821 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
    26881 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
    26941 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
    27001 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
    27061 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
    27121 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
    27181 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
    27241 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
    27301 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
    27361 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
    27421 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
    27481 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
    27541 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
    27601 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
    27661 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
    27721 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
    27781 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
    27841 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
    27901 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
    27961 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
    28021 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
    28081 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
    28141 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
    28201 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
    28261 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
    28321 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
    28381 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
    28441 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
    28501 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
    28561 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
    28621 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
    28681 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
    28741 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
    28801 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
    28861 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
    28921 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
    28981 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
    29041 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaaca
//
LOCUS       DAZBTH010000022        28532 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000022 DAZBTH010000000
VERSION     DAZBTH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.022"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1659
                     /locus_tag="ACHGNW_001000"
     CDS             22..1659
                     /locus_tag="ACHGNW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925332.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1804..2544)
                     /gene="truA"
                     /locus_tag="ACHGNW_001001"
     CDS             complement(1804..2544)
                     /gene="truA"
                     /locus_tag="ACHGNW_001001"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6925333.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2735..3370)
                     /locus_tag="ACHGNW_001002"
     CDS             complement(2735..3370)
                     /locus_tag="ACHGNW_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN6925334.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3517..4737)
                     /gene="sstT"
                     /locus_tag="ACHGNW_001003"
     CDS             complement(3517..4737)
                     /gene="sstT"
                     /locus_tag="ACHGNW_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN6925335.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4956..5954
                     /locus_tag="ACHGNW_001004"
     CDS             4956..5954
                     /locus_tag="ACHGNW_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925336.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6150..7985
                     /locus_tag="ACHGNW_001005"
     CDS             6150..7985
                     /locus_tag="ACHGNW_001005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6925337.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8045..8761)
                     /locus_tag="ACHGNW_001006"
     CDS             complement(8045..8761)
                     /locus_tag="ACHGNW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN6925338.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8988..9530)
                     /locus_tag="ACHGNW_001007"
     CDS             complement(8988..9530)
                     /locus_tag="ACHGNW_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN6925339.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9535..9762)
                     /locus_tag="ACHGNW_001008"
     CDS             complement(9535..9762)
                     /locus_tag="ACHGNW_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN6925340.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9841..11721)
                     /locus_tag="ACHGNW_001009"
     CDS             complement(9841..11721)
                     /locus_tag="ACHGNW_001009"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6925341.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11910..12524)
                     /locus_tag="ACHGNW_001010"
     CDS             complement(11910..12524)
                     /locus_tag="ACHGNW_001010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925342.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12666..13226
                     /locus_tag="ACHGNW_001011"
     CDS             12666..13226
                     /locus_tag="ACHGNW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6925343.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13531..13845)
                     /locus_tag="ACHGNW_001012"
                     /pseudo
     CDS             complement(13531..13845)
                     /locus_tag="ACHGNW_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14099..15734)
                     /locus_tag="ACHGNW_001013"
                     /pseudo
     CDS             complement(14099..15734)
                     /locus_tag="ACHGNW_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16289..16852)
                     /locus_tag="ACHGNW_001014"
     CDS             complement(16289..16852)
                     /locus_tag="ACHGNW_001014"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6925344.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16867..17910)
                     /locus_tag="ACHGNW_001015"
     CDS             complement(16867..17910)
                     /locus_tag="ACHGNW_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925345.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18043..18447)
                     /locus_tag="ACHGNW_001016"
     CDS             complement(18043..18447)
                     /locus_tag="ACHGNW_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6925346.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18680..19315)
                     /locus_tag="ACHGNW_001017"
     CDS             complement(18680..19315)
                     /locus_tag="ACHGNW_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN6925347.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19581..20633)
                     /locus_tag="ACHGNW_001018"
     CDS             complement(19581..20633)
                     /locus_tag="ACHGNW_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6925348.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20646..21584)
                     /locus_tag="ACHGNW_001019"
     CDS             complement(20646..21584)
                     /locus_tag="ACHGNW_001019"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6925349.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22035..22685)
                     /locus_tag="ACHGNW_001020"
     CDS             complement(22035..22685)
                     /locus_tag="ACHGNW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925350.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22785..23294)
                     /locus_tag="ACHGNW_001021"
     CDS             complement(22785..23294)
                     /locus_tag="ACHGNW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFN6925351.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23495..24364)
                     /gene="scrK"
                     /locus_tag="ACHGNW_001022"
     CDS             complement(23495..24364)
                     /gene="scrK"
                     /locus_tag="ACHGNW_001022"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6925352.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24384..25505)
                     /locus_tag="ACHGNW_001023"
     CDS             complement(24384..25505)
                     /locus_tag="ACHGNW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6925353.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25507..25902)
                     /locus_tag="ACHGNW_001024"
     CDS             complement(25507..25902)
                     /locus_tag="ACHGNW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6925354.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25921..26718)
                     /locus_tag="ACHGNW_001025"
     CDS             complement(25921..26718)
                     /locus_tag="ACHGNW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6925355.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26720..27508)
                     /locus_tag="ACHGNW_001026"
     CDS             complement(26720..27508)
                     /locus_tag="ACHGNW_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6925356.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27522..27998)
                     /locus_tag="ACHGNW_001027"
     CDS             complement(27522..27998)
                     /locus_tag="ACHGNW_001027"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6925357.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28070..>28532)
                     /locus_tag="ACHGNW_001028"
     CDS             complement(28070..>28532)
                     /locus_tag="ACHGNW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6925358.1"
                     /translation="VEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSL
                     TNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNT
                     LEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         8987 a         5722 c         4387 g         9436 t
ORIGIN      
        1 acttttaaat aggagaaaaa aatgaaaaaa aacaaaaacc aaataataga tatcattttt
       61 atgttatttc taagttttgc ttacgtcatc cctctattca tgtctaaggg aatctatcat
      121 agtgtcaatc aggatactta ctttcattta tctcgaatca taggactaga taatgtatgg
      181 agttctccag taaacttcaa taacttcgat catcatggaa caatgatgaa tattttttat
      241 ccatggctta ctctttatcc agctttttta ttttataaaa tgatgggaaa tcttgtttta
      301 agttataata tctactattt ttttatcact tttttaacaa tggtcgtttc ttatttttct
      361 atgaaacaaa taaaaaacaa tcgttatatt tcgctgttat tttcgattat ttatacattt
      421 tccgcctatc gtgccattga catattccat cgtgcttcat tgggagaagc tgtcgcttta
      481 acatttttgc ctttgatact aatggggtgt tacgaaattt atattagaga ttaccaaaaa
      541 tggtactggt tgtctattgg catgacgcta gtagtgtata ctcatttact ttctgtcgct
      601 atggtttctg tatttattgg aggaacgttg tttctttctt tttatttttg ggaccaaaaa
      661 atcgctagac tcttaagtct tttaaaagct accgtcctca cctttttttt aagtgcggga
      721 ttcttaattc cttttattca acaatctaga gcacaagaat tgaaagtccc actgggaaaa
      781 gaattaagtg gtatggctcc tagtgatatg ctgacacata ttttaaataa taactataac
      841 aactatacta ttggattatt cttatttctc ggattgattg gtgcttttat ttttataaaa
      901 aaattaactg ctgatgattt atttattttc tttcttggtg tatttgttct tttttgttca
      961 accaatttat ttccttggca actttttaac catacaccag ttaagagtct acaatttgtc
     1021 tggcggttaa atggtttttc ttctttattt atagcctata cgatgagtat agtgatctat
     1081 tatatttttt ctactaaaaa taattcatgg aaaataatgg tctttacgtt tcttgcacta
     1141 atattgcatc tctcaggagt aagcaattct atccatgcta acaaagattc attaacactt
     1201 atagcacctg aagacgctgt tgctatagct gaaaattaca atcatactga ttatgcaaac
     1261 aaagagtcaa tctatcatcc tgatatgatt aataatgatc aatatttact aggtaaccag
     1321 gaaataaatc caacaactca ttttatgagt aacaaactta caattgaatt agataattcg
     1381 aataataaaa atactgtctt gacgacacct atttatcgtt ataaagggca agtggcttct
     1441 attaatggca aactagtaca aacaaaatta tctaagtttg gcacaactga attaactatt
     1501 cctccaggta taaataaagt cgttattact tatcagtaca caaaactagc aattgcttca
     1561 agatatttat ctatcgttac tttgatttta tttttacttt atcgatttac tttttctaaa
     1621 caaaaacaac cgagaagaga agtacaacat tctcactaga tttttattta agaaagactt
     1681 tttagctgta cacataaaca ggctaaaaag tcttttttta tatttcttta atttgcttca
     1741 tacattattt aacatcgata tttatactgt gaaacatgcg tttcatccat tatcaaagta
     1801 tttttatcga taataaactt catccaaaaa cagaccttgt gcagggacgg tttctcctgc
     1861 atgttgccgc actttgcttt caaatatttc atcgattgtt tctatcggca tggcacctgt
     1921 gccgatttct agtaatgtcc ccatgataat ccgcaccatt ttatataaga atccgtctcc
     1981 gacaaaagta aaatgaagca tttctccctt tcgttcgata gataattcat caatcgtacg
     2041 cactgtggat ttttttgatt ttttcaatgc agaaaaacca atgaaatcat gtgtacccac
     2101 taatttttcg caagctgcat tcagcttgtc catatccaac tcttgaggga aataaaagct
     2161 gtggtttcgc tcaaatgctg aaggaatcac gttgttccaa acataatagc tgtatttttt
     2221 gcccaccgca ttatatcttg aatggaagcg ctcaggcatt tcttcgattc ttttgatgac
     2281 aatatcccgc ggaaggtaac gattcataaa gtcgatcatt tctgccagtc caatttctgt
     2341 gtttgtttta aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga
     2401 tccaataatt tctatctctt catttgtcat ctgatgcaat actttttcga tttttccttg
     2461 aatggtttta tcgtcatctc caagtctttg ccatcctgaa tatctttttc cgtcatattc
     2521 gattgtcaac ttgatgtttc tcatatttta gtggctcctc tgtcttccat ttggtcttct
     2581 ctctaccttt ttaagattgt agcacaaaat atagctcctt gcttctctaa gacagaagct
     2641 atacaagaaa actgtggaaa tatatatttc agaaaaacaa aaaacagcca ccggatgaag
     2701 tatccgctga ctgttcaatt tgacggctca agttttactg tttgagatgc aagatactca
     2761 acctggcaat gggcacataa ttaaagaaaa aactgattct ttggcaaaaa cctattttat
     2821 tcagtaatgg ccaagaattt atagaaggaa ttcttgccag tccataaatc gccgcaaaaa
     2881 gcaaactgat cacagacagt ccaaggtata agtggcttac gtccatttgc ccattttttc
     2941 gatagatcaa gaaaagaaga actggaaaca ataaaaaacc actgtaacct aaacctgatc
     3001 cgatattatg gacccatgtc gagacattcc acgaagattc atgggtattg atactgaaca
     3061 aacctgtaaa gatacaatca cctataccat agaacgcgat ggctgctgta actagggtag
     3121 ctaacacacg agatgtacta acaaatgcag catacaatgc aggcaatgac aggatgaaaa
     3181 agatccctga aaccacagac caaataagaa acgcttttct tacaggactg gaaacctctc
     3241 caaatacact gatcacgctc ttcatttgat ctaattcagg ataatacaat cctaatacgt
     3301 acggagtcaa aaaatcactc agcacgccta ataataaaaa gaaaaaacca aatttacgta
     3361 aaaaatccat gatacgcctc caataacaat tacctatgct tttaattgtt attataaacg
     3421 actttatctt gatttaacag aaacctctat ttacacgaac atctatataa ctcattaaaa
     3481 actcgaatca ttttaaaaat actaaatatc taacgttcaa cgttcactta atccatctaa
     3541 acaatctttc aatttgattt tttccccact tcttttacgc gcgcctagct ctcctattgc
     3601 agtaaacaac agatcacttg acgaatttaa ggcagtttcc attgaatcct ggataacacc
     3661 gataataaat ccaataccga cgatttgcat cgcgacatca ttagagatac caaacaaact
     3721 acatgctaat ggaatcaata acagtgaacc gcccgcaaca cctgaagctc cacaagcagc
     3781 taaagcagac aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga
     3841 atgtacagca gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt
     3901 agcacctaac gggatcgata tggaataaga ctcttttgtc aatttcaatc tttcagccaa
     3961 catcatgttt atcgggatat ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct
     4021 ttctttcaaa caaaagaaaa tcaatggata gggattttct ttaataaaca aaaacgtgag
     4081 gaatgggtac acaactaatg cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg
     4141 cagatactct gccaatccag cgattccagt cgtagcaatt gattgataaa ccaatcctaa
     4201 aatccctatc ggagcaaatg caataatcac ttgtacgact tgtgacaacg ctatagatat
     4261 ctgatccacc aactcttttg tcgattctga acgtgctcga aaagctagtc ccaacaatac
     4321 agcccagaat aagacgccaa ggtaattcgc atcaataaga gattgaacag gattttgtgt
     4381 gacattcgtc aacatagtag ataaaacact ttccaaactt tgcggtgctt tttcagatac
     4441 tgtttcttgt aaagtaatag gaacagtaaa aattgaactt acaataactg ctacaagtgc
     4501 agaaagcaat gttcccacta aatataagat caaaataggc ttcacgaatg tcttattccc
     4561 aattttatgc ttggcaattg acgccatcgt caagaaaaaa accagaatag gtgcgattgc
     4621 cttcaagcaa ctgacaaaca acgttcccaa tattgaaata aatgtccatg tggggaaaaa
     4681 gataccaaac aatattccga ctaaaatacc aacaacaata cgttgaacta acgacatatc
     4741 caaaaacttc tctaacaaat gaccaccctt tcacaatttt aacaatcatt gtatcatttt
     4801 atgcaaagaa actaaaggta taattttcta aaaattctaa caatacaaac aaccatttct
     4861 tgctaatttt gtactttttc tgtctacacg ttgttttttg agatattcta agttaggatg
     4921 ttattaatgg gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga
     4981 ggtgtttatt cttgaaaaaa ataaagaagg agagttatgg cttcctgaca atctttgtcg
     5041 cttgtataat gatggtttta cagcgtatat tgatttcagc agtcactatt cctacatatg
     5101 caacaaaccc tggtcgattc tacatctata catttttaca aacttccgtt gttattggtg
     5161 caaatttgat cccactttac atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt
     5221 tacattattt aagtcgtttt gcctttatat tcctaatagc cacactagtt tgcaatatct
     5281 ttttttacgt acaagctggc agcttgaata ttcgtgatta ttggcttatc ctaacaccta
     5341 tcagtcagaa ttactttact tatgctgttt cttgtacttt gcttttttgt agtattcctt
     5401 atacagttgg atttttaaat aaattatcga aaacaacaat taaatatcta ctattatttt
     5461 taacaattgg attagttggc tgctctactc tatttaataa agacccgttt gcattacaga
     5521 atggacagag tattttatgg attttttatt tatttttaat gggttatggt ttgaaagaat
     5581 ggaactggaa agaaaaggtc cgctataagc ttcctcaagt atttatcagc ttgattgttt
     5641 tattcggttt gattattttg atgacacaaa tttcactcat catcagagga aatacagata
     5701 cagcgctgag gttttctaaa ccatattctc tcttcgcaat gtattatact ttctcttcgt
     5761 ttttattttt agaaatgcta tctgataaat taggtataaa aggaagagga aattttcttg
     5821 ctgtattttt gattgtcact caagttatat gtaatttccc tttagtttct tatcgcgtat
     5881 ctacatttat gaaacgtgaa tttccaaact cagggaaggc atggctgata aatatcgggc
     5941 agttttttat ttgatattta ttagcagtgt ccctcctagt tatcttttta ttttatatac
     6001 aaaaaatccg tttcattcat cgtttcatcg aatacttcgc ttttgattcg attgaacaat
     6061 taactcagcg ttggaaaaaa agaattcatt ggctttttgt tcataaaagt tattttttag
     6121 ttgcagcatt tttttactgc tttaccttcg tgcagatttt cgttttagaa ccaaaagaag
     6181 gttggaaaga aactatccag gttgccttaa aaatatttac tcagcgacaa gcacccatga
     6241 tcctgacaat catcattatt atgggatttt tcttcttact attgttgatt tccaatcgtt
     6301 tttggtacgc cttgactgct accttgatca ttaacttact gctcactatt tcgacagtaa
     6361 tcaaaatgga aatgcgggaa gagcctgtct ttccttcaga tttaaaaatg ctgaccggat
     6421 tgtcagaact attatcgatg gttagcccag tcttattaat cgtgggcgga ttgatccttc
     6481 tcctattatt gattactagt attattgtac aacgtagact acaaaaacag tattccttga
     6541 agattcattg gaaacgtcga tttatcagta tcgctgtatt attaggatgt ttttcgggtg
     6601 tctttttcat taatcacaaa aattcgcctt ctttcttgct gtttaattta tttaaagtaa
     6661 acaagacttt ctttaaccag caaggcgccg taaaagataa cggaccagtc attcaattcc
     6721 taaacaatat cgacattaaa atcatggaag aacctgctgg ctattcgaaa gaaaaaatta
     6781 atggaataat gaaaaagtac aacacaaagg cagatgaaat caaccaaaca agaagtgact
     6841 ggttaaacaa tcagacgatc gttctcaatt taagtgaaag cttcagtgat cctgcacgtg
     6901 tccctaatct gacagtacct acagacccta ttccttacat caaaagcatt ataacagaag
     6961 gaacaggagg attgatgtta tccgtcggtt atggtggcgg aacagctaac atggaatggg
     7021 aaggactgac aggcttgagt atcagtaacc tgtctgcttc gttagttaca ccttataccc
     7081 agctagtgga gcgtcagaaa ataagtccaa atattaccga tctattcgat gaaaaaatag
     7141 ccatccatcc ttttacagct gctctttatc gcagaaaaca agtgtttgat aaatttggtt
     7201 ttgacaggtt ctattattta gacagtccgg ataaattaac ttatacggat aaaatagaac
     7261 aaagcccacg catatcggat gattcagcat acaaagaaac cttgaaagct ttacaaagca
     7321 atacggaaac gtcacaattc atccaattat ccactatgca aaaccatatg ccttatgatg
     7381 attactatag tgaattgaac tacactgctg aaggaactgc tgtcattcct aatagaagaa
     7441 aagaattaca aacttatatg caaggccttc actataccga tacagcagta aaaaaattca
     7501 tccaagaaat cgacaaaatc gataaaccga ttacctttgt tttttatggt gaccatttac
     7561 ccgcaatcta tagtgggaat gattcgaaaa aatatggatt agaacagcac cagacagatt
     7621 atttcattta taacaacaag tatagtcgtg aacaagcagc tacgttaaat aaagaagttg
     7681 ttgctcctaa taactttggt gctatggctc ttgagcatgc gaatatcaaa gtaactcctt
     7741 actatgcgtt gttgacagaa gtatctaatc atctacctgc attaacaatt gatcctacag
     7801 aaagcttgtc taatcgtttt aatggaaaac aggtctttgt tacagaggat agtcaaatta
     7861 tccaagaaaa aaatttaact gagaaacaaa aagaaattct agaagattat cgtctgatac
     7921 aatatgatct tgttgcaggt aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag
     7981 actaacaaat aaactgagca aggggttgtg ataaaagaca tgtcacaacc ccttgtttat
     8041 ctcattaatt tgcttgtcca taaacgattt gattaaagcc atttttcaat tcattattcg
     8101 ctgtctcgtt gaagtaaaga taattatcgg gattcccctg attttgttgt gtcaccaata
     8161 cttttggctg attttgctga aacccgaata gatagacgtg ctttttcaga tatggttctg
     8221 tctctgcatc ggaatagacg gctgtgattt gatagtctgc ttgacctgtc ccactttctg
     8281 accattctgc agaaacaggc actccaccaa tagccatctt ccactccccg ttcaaaatag
     8341 cttgagggac tttaagacca tataagtcca cactcatttg attcgtataa cttttgtatt
     8401 gttgtcccat tgtttggccc catttggtca taaatgcctc taattgtgcg gatttgtctg
     8461 cattccacag tgtttgttca acttgttcgc tgctttctga agaggaagat gaagcctcta
     8521 cagaagaact tgaagtagat gtttgagttg tttcagtcgt tttggaagac tctgacgaag
     8581 tagaggtatc tgctgtttct gtacttgctg cagttgactt gctctctgtg ctagttgttg
     8641 tagtagttgc aactgttgct ttcgctgttt cttttgttga ttcttttttc gattcattcc
     8701 cgttggtaca accagctaac aaaaaagcta gaccaattgt tactaaaatc ctttttttca
     8761 attaaaaaac tcctttcgat tactcccact tctttattct atcaaataag caatgtgatt
     8821 ttattgattt ttcgtatttt ctcatcaatc tttcaaatct tctaaaaact gagaagcacc
     8881 cgctgatgtt gtctgtaata catcagcggg tgcttctttt attttaatct tcgtgatttt
     8941 ccaatttacc tcacgcttta tatttgtatt tgaagaaaca tagactttta gtcatcatcg
     9001 tcttcctcat ccaaatcttg tcggacagtt tttccttgga gtctttgtac ttggtagatg
     9061 atttctttat tgaaacttaa aagttcaaca agcaattgtt gcaagccata tttttcttct
     9121 ttttcagcta atttgattgc ccgtgtgata aagacatttt ggaaatcaaa atcttttgca
     9181 aagttttcca acattgttga ggctggtaaa tatttgtttt gaccatgttc gtaaattttt
     9241 ccataacgca tgacttcttc taaagtagac ggaacgattt cattttcaat cactaactca
     9301 tgagagattt tattgaacca tctcgcttct tgatcagcat attctgaaaa aagggtactt
     9361 aattcttgcg aagatgtacc caaaacatac aattgtgctt ggatcaattt catttcttct
     9421 aatttaaaat ttgctaatac atgccccacc atggcgccag cagttggttt atgatggtcg
     9481 atatctgctt gttttgtttc ttgaacaaat ttttcttcca ctatattcat tgatttacgc
     9541 ctcttttact tttgctgatt ggacttcata acctaatttt tccacaactg cagctaattg
     9601 ttcagggtgg acttcatctg gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac
     9661 tttcgtattg cttaccccag gctgttgaga aaccgcatgt tcgatttttt gcatacagct
     9721 tggacaagct agtgtatcta attgcataat aatttttttc atcgtagttc ttccttcctt
     9781 tttcttgata gtcttattct agattacagc ttccataaaa aaattgattc ttatcaagtt
     9841 ttacgatttt tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat
     9901 aacgatcaag atactagctt cgtgaacgaa catcccacta gccatgtaaa taaacccggc
     9961 aaataagccg ttgagtaaaa ataagacgac accaatggca atcactacat tttcacgggt
    10021 atttaaaaca gttttcttag ccaagcggta ggcatgaacc aacgattcaa aattagattg
    10081 catcaagaca acgtcactcg tttcgatagc aacatccgtt cctgatccca tagcaatccc
    10141 gatatcagcc gcagccaaag agggagaatc attgattcca tctccaataa aagcaacgcg
    10201 caatccttgt tcttggtatt ttttcacaaa ttgaaccttt tcgtctggta ataattcagc
    10261 atggacttca tcaatgccta acatatcagc tacataatca gcagtcatct gattatcacc
    10321 tgtcagcatc actaactgtt ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac
    10381 ttctggtcga atcacatctg acacacctat gatttgagtg atttttttgt cgattgctac
    10441 aataatgacc gagctgcctg tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt
    10501 gagagaaagt ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg tatgaccatc
    10561 aatctcagct gtgattcctt gtcccttgat tgtctgatta tctttgactg ctaattgttg
    10621 aaaatcaatg tctttctcct gagcataatg gatcacggcg cgccccagag gatgatcgga
    10681 taacgtttct aatcgagcca ctaaagctaa gctgtcccct tcatcctctg tatagttctt
    10741 gatagtggat acagcagtag ttcctttggt caatgtacca gttttatcaa agacaaaagt
    10801 atcgattttg gaaaaagtat ccattgcttc accgcctttg actaagatgc cccgtttcgc
    10861 tccattaccg attcctgcta cattcgaaac aggcgcacca atgactaaag cgccaggaca
    10921 accaagaacc aaaacagtga ttgctaaacg aaaatcacga ctaatcagac caacaaccaa
    10981 cgcaataagt aaaacggttg gagtataata ctgagaaaat cgatcaatga atttttccgc
    11041 atgggatttg gtatcttgtg cttcttctac taattcgata attttggcaa aggtcgtatc
    11101 atctccgacc ttagttgctt caatcgtcac gtagccatct gctaaaatcg ttccagagaa
    11161 gacttcatct ccactgattt tgacgacttg tcgtgattca ccagtcactg cggcttcatc
    11221 cgcataccct ttcccgtcaa tgactcgtcc gtccacagga atagctccac cagtttttac
    11281 taagacgata tcgccttctt ccacgtcatc gatttccact tcttcagtcg ttccatcttc
    11341 ttggacaaca ctagcagtag atggggccat ctcggtcaaa gccttgattg actcccgcgt
    11401 tttggtcaat gttttttgtt ccagatagct accaaacaaa aacaagaatg taacgatagc
    11461 tgactccttg tattctccga tcataaaggc accaagcaca gcaatagtaa cgagtaaatc
    11521 aattgagata actttgactt tcactgcctg atacgcatgg atcataatag gtatgaccgc
    11581 aataatagaa gctatggcta gagcaatgtt atagaacaag acaaaaccag taaattttcc
    11641 gataaaacca aaaataatca acacagctga taaggcggtg atatgatttc gatgctcttg
    11701 gatccacttt tgtaatttca tctttctccc tcttttcctt tcattcttac gagactaagt
    11761 atagatactt atccatcata aaaaattgac cactatcaag tttagtcaaa aaggcggttt
    11821 tcttttggta gaatacacta caaaaaatag ttagcaagaa gactcactaa aagtgatctt
    11881 gccaactatt gtcgtttcta tcgaaatttt cattgaatga ttttcctgta cagcatacat
    11941 cggagatcta tttctttatc taagattttt atcttgctaa ttttttcctt tagtctttca
    12001 aatccacgtc gttcatagaa ttgatacgta cagccactat ctgtaaaaag gaatatttct
    12061 tttcctttct ctcttttttc aaattctgtt agcaacttgg taccgatgcc ttttgttttc
    12121 aaatcaggat ttgcagctaa aaaaatgatc tctccatctg gcgaatgctt ctttaaatat
    12181 gctgcaagca tttgttcatt tgctgtctcg taagctcctt tactttcttt agcaaatata
    12241 ttttgtaaaa aatcgaatat cttaacatat atttgctgtc caaaagaacg atgtttcttt
    12301 ttttctcctt ttatctccgc cagcaaaaca cctgccaact cgtcctctgc atacaaagca
    12361 agaatttgag ttgctcgatt caactccata tacaaaaaat atcttccgta aagattcagc
    12421 agaagttcat tttccatata tctctcaaag ttcattcctt caacagcaaa atagatagct
    12481 tttttgtaat ctttcttttc taagggttta aaagttagtt tcatcatttc gcctcctcaa
    12541 cttcaatgat tgtgttatac ttacattata atagacacag tgtctactat ctataatcaa
    12601 tcatctataa gtttgtcgat taagcgacag ctttgctttg atatgtcgga taaggagata
    12661 ggtaaatgaa aaagacagat agacgcgtca aaaaaacaga aaaagctttg gctgaaacac
    12721 tatccacatt attagtaaat aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa
    12781 cggcagatgt acatcgttcg actttttata cacattataa ggatatctat gatctatatg
    12841 atcaattaga aagcaacttt tttcgggatc taaatgaaat tctctcttat gatccagccc
    12901 atagctatga ggaactatac actcgcttga ttgactatgt ttacgcaaat acttttttat
    12961 atcgcttatt tatcctcaac catggtgata tttcgtttca gaagaaagtt aaacaagtta
    13021 ttgaagaaaa ctatctgaac atatggctat atgaagacaa gaaaacgatt atcacagaag
    13081 aaatgagatt ctttactacg taccacgtcc aaggttgtct atccgttctc aatcggtggg
    13141 tagaaagcaa ttacaagtac tccaaaaaag aattattaga actcttacag aaactgaatg
    13201 atcacataga gaaaatcatg ccttgaataa aaacatacta agcttagtta agaagaaccg
    13261 taaccttctc gtataacaac tgaatcttat ttagccaaat tgtttactga agattttttg
    13321 agtaaaattt ctataattcc tacttattga aaagtagttt gtagtgctgc ttctccctct
    13381 ctaagtgata tatcttgttg taccaatcaa attcacctca tcattaataa attactttta
    13441 ggctaattta ttacaactta taggttgaag ctgtaaccaa tttatagtca tattatattt
    13501 ttatcatcca gtttattgaa ttcttctcgt ttacctcaat tcattttcaa ggggaatcag
    13561 ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt taaatggttt
    13621 cgcttctgga tgtttggctt tatatccttc gtaatcctta ctgttgataa ttgctttcat
    13681 attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta attttttctc
    13741 agaaacaaac cactcattgg aaaagacatg aatcaaatca tcacttactt gtgtaaaata
    13801 gcgacgttta atgacaaaga tatcttcatt gctatctagt gtaccttcat ttctttgtgc
    13861 ttaaattcaa agataatttt ctcaataaag atttttgatt ccagtaagat tcctttactc
    13921 cttttacaac atagcgttgt cctttgtttt gaaacatctt cttcccctct ttgccattaa
    13981 aaatagccca attctctata aagaagatta agcttcatta ttataatata aagtttaaat
    14041 ttggtttatt acttactttt tcttattctc tagtaagcta atattgaaaa aaatgtaatc
    14101 agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa agaaagaggt
    14161 aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg gaagaaactt
    14221 caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc aatctctctt
    14281 ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag cgttattttt
    14341 tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg ataaggcata
    14401 gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt gtattgtaat
    14461 gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta acgaataaga
    14521 ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc atagtttgct
    14581 tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt aactactttt
    14641 ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga gccaaacagt
    14701 acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt attttgtaat
    14761 aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact aaggtaaatc
    14821 actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa agaaataata
    14881 ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat caaactatcc
    14941 gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat cgattgaacc
    15001 atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt cattcctact
    15061 gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc acttactaca
    15121 aagccgccta agactagtaa gccaacggct attacactca taaaagtgga aagaacatgt
    15181 gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt tttctcataa
    15241 atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat cgcttcagca
    15301 atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata taaacagcta
    15361 aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa catcagcatg
    15421 atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa attattactt
    15481 atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata attcactccg
    15541 ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga agataaccct
    15601 ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg aacatttcta
    15661 aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa tattattttt
    15721 ttctttactt gcattactta attatatact cctttcatgg attgctttat ctagtataac
    15781 ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca ctaaaattat
    15841 aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat tgcttaacta
    15901 atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt gcgatctctt
    15961 tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg gatatgacta
    16021 tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag gcgctgcgta
    16081 tcatgagtga atgatattag gaaaacttca taaaataaat cttttataca tttccacttc
    16141 tagatggtca ttcatgttag aagaatcaaa ctattttttt actgtcgtaa taatattaaa
    16201 acactagaac aaatcaatta aattccataa aatataaata atagacctag catcatctgg
    16261 tataaaatgg atgctaggtc cgttatattt actcaaaatt gattctacga atagatcttc
    16321 ttatcatttc tattttagta gtcatgcgtt gttgttcttc taaaggtggt aatggtataa
    16381 gtagtttaga taaatttgta ccatttacat ttggttgccc agttccgtaa gaaatttttt
    16441 ctaataactt ccaatataat ggcgagtcta agaagcagtc tacgtattct gataataatt
    16501 tctcaactaa cctaattcga atcaagtaag aagcaaaaac agattcctca cttatttcct
    16561 taaccaagaa actttttccc atagttcctc ctgtccttgc tatcaaaatg tcattttctt
    16621 ccaatcttaa atcaactagt ttagaattac taatatcaca gtaaggcaca ctggaccaat
    16681 ttacacgtcc ctcttgtata tctgtgattc tcaaaaactt aacattgcct tgttttttcg
    16741 ccgaatctgt atagccatat tgaatcttag tagaaatact tcctaaataa acccatagtc
    16801 gaacatcgct agatgatttt tcttgtcgtt ctttatgaac aacagtgatc atcttgagtt
    16861 gtccgactat tttttagttg cagcaatctt tttgggacgc ttatttgcat gaagcaacgt
    16921 tgctaagtct agtaataaaa cattggctaa cacaggattg gctttctcgc gatacgctgc
    16981 ggcgccactt tcaaacaata ctgctagacg atcttctaca acaacgattt gttccaaata
    17041 cggtggaaat ttgattcgtt ttaggatccg gctaggagag tcccgctcgg acactaagag
    17101 ttccgaggta taaggaccat aagaacgact ggctaagaga taatggccat aaaaagccac
    17161 accttgaatc ttatcagatg tttttctgct cattgtcacg gttggttgga ttttgtcttt
    17221 gcggcgaatt gctgttttca tttcagctat cgtcggcaat agatagaaag tcaactgtcc
    17281 ttcacctttt agtgtgaagt tgccagatac cagttgatca ttgttttgag tcaaatacga
    17341 tgcttgtgga atctctgaga gatcagttgt cagccaataa gcgattggct gtcccgtctc
    17401 ttcagatgta tctgctaaga tatcgatcaa acgtaaagcg gctactcgtc cagtagcaga
    17461 tcctgtgatt gtcaaccata aaagttcccg ttttgtatca tatactaagc cccctacatg
    17521 tggccgttta ggtaaaatca gcagtttgat cggctgtcct gtttctctat ccagtacatg
    17581 aataatagaa tgatgctgat ggtcatagca gtaggcagaa ataaacacaa aatcttgaga
    17641 aaaagccaac ccttgcggcg tcatccattg acaaacctca ttttcgccgg tttccggcac
    17701 caccattctt gtttctgtta atccaggaat gttatattgt tttgcttcag ccttttccca
    17761 agctgcttga acaataggat accgttttaa ctttttttca atatcctgat gtatccgttg
    17821 ctgcttccaa tgcttaaact ttaggacata gtaaacaaga aggccaatga ccagcaacac
    17881 taaaatcatt attgctaccc gctgtgtcat ttttttgcct ccttgttcta ttcgatcaat
    17941 cgtccatata ccgctattaa ttaccgtaac tacatgtttc ttttactagc gtttctttat
    18001 aggatctttc ataatctcat ttatagtaac attaagaatc ccttattcta ctatagtcac
    18061 ttccacatca atattgcctc ttgttgcttt agagtatggg caaaatgcgt gggcttcttc
    18121 tgcgattttc tctgcttggt ccttctcaag tcctggcaaa gaaacttcta aaacgacccc
    18181 gatttggtag cccccatctt ctaattgaaa taaagatacc cgtgctttga cttcagaatc
    18241 agactcaaga ttattttctt ccagcatgtg ttgaaccgcg ccgttgaagc aagatgcgta
    18301 tccagccgca aaaagttgtt ccggatttgt cccttcacta tggattcctg ggcggtcgat
    18361 cttcatgtgc atacttccgt ttggtgcctc aacttcgcca tcacggcctc cacgattgat
    18421 cattgctgtt tcgtacattt ttttcattga gatcatcctt tctttcgtcg ttctatatta
    18481 agtgtaacga gtatttttta atctgacaaa ggtaatgctt gcattcggcg acatcagtta
    18541 gacaacatcg atgatcagtg atagaagcag tttatctata caaaaaaaga agggttctat
    18601 gatatttggt gtccttactc gccttgagta acaacaccaa atatcataga accacttaat
    18661 cagcttcaca ctgctttttc tactttcgta gttgaaaaaa acttaaacaa atcatcggca
    18721 aatagttaaa gaaaaaactt aatcgttgaa agaagcccaa ttgcttaaag aaggcaaact
    18781 gactaatcga cggaatccgt gctaatccgt aaagacctgc aattagtaaa ctaataactg
    18841 ttaaaacaag ataaaaatgg ctaagcgtac cttgaccact ttgacgataa agcagaacaa
    18901 gtaacaatgg gaaaagtaga aagcctgcat agcctagccc agaacctata ttatggatcc
    18961 aagtcgatag attacaagag ctttcattcg tattgatact gaataagcca gtaaaaatac
    19021 aatcacctat cccatataaa ccaactgttg caacaacaag tattgcaagt gtttttgatg
    19081 ttccaacaaa taggtgatac aacgcaggca acgaaagaac aaaaaaaagt ccagatacaa
    19141 ctgaccagac caagaaagca cgtctgactg gactatctac gtcaccaaac acactaatta
    19201 cggtagtcat ttgattcagt ttgggataaa aaaggccaag gatatacggt gtcgataaat
    19261 cactcatcac acctaataat aaaaaataaa aaccatattt ttgcaaaaaa atcattttct
    19321 tcccctactt tttagtgatt attcgataca tccaacaatc aaattttttc tcattttaac
    19381 acatattttt attctagttg tcttttgaac aatcaataac ataaaggtga gtttcttcta
    19441 cattttaaaa aatcgaatgg cactgaagag ttcagttgtc agcatcaatc tcctactcaa
    19501 aaaggcaaaa aaacgtaagt cagatgttgt tgacatctca cttacgcatc cattgattga
    19561 aaatttctta gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg atcatacagt
    19621 ttaaatactt tctcgcataa aaattgagta attagtgccg caaaaacagg gatgactaac
    19681 caacaaagaa caacaagaaa gccatttagc cctgcatcta aagaagccaa cggaccaaca
    19741 agtccgacca cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa tagagcgaca
    19801 ggaatagctg taatcactgc actaactaaa caaggaataa gaatcacagg ttttctaaat
    19861 agattaggca tcatcatctt catagcgcct aaagcaatcg caattgtcac acctgactta
    19921 ttaaccttcc aagagttcac tactaaaaca aaagtggttg cagctacgcc catcgcagcc
    19981 gcaccagcag aaacaccatc tagttggatt gccattccga ttgctactgt tgaaatagga
    20041 gagataatca aaaaggcaaa ggaacaacca atcaagatag acattaacag cggttgtaag
    20101 gtagtgaagt tattaatcaa tgatccaatt gcagaagtaa ttgctgttac aaaagggaat
    20161 aaataaaaac caagtaaacc tgcacctaca cctacaacaa tcggtgttaa cacaatcgac
    20221 actgacccaa aactatttcc aatcagtaaa atcagcccga cagcaatcga tgctgtcagc
    20281 atcgtattaa taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc acccaaatca
    20341 ggtgtgaatt tgactacacc agaaccaaca aaagaagctc cggctacaac catcattctc
    20401 atcggatcaa atccaaattg taaggcaatc aaaccaccaa tgatcaaagg tgttgctagt
    20461 tgaaaaataa ctgctaactg tgcgatcgtt accgcaaagg gatacgcact aaaatatttt
    20521 aaaatggcac ctaatactgc attaggaatc aatccaataa tgattcctgt tgctgtacca
    20581 gcgaggactt tattcagaaa aattttcggt gatatttttt gtgaagtttc cattcaatct
    20641 ctcctttatt ttacgttcaa actatcttct tttgcatgga caagctcagt caagaaatca
    20701 caataaggcg tcgcaacacc atatttcttc ccttttctag aaattgcgcc attgatataa
    20761 tcgatttctg ttaatcgatg gttcttaatc aagtcttgat acattgaagg ataatgtaac
    20821 ccaattgttt ccgggtcaaa acaagattca caatgtgcaa tgacttctgg gacatctagt
    20881 tcaatcttct ctactgctgc tactttggca aactcattga caatcgtcgc caccatttta
    20941 tgagcagtcg atgtttttcc tagctcagcc atattaacgt ctaagattgt gcatagtccg
    21001 ttcattgttc cgttcacaca tgctttgcga taaatagaat agtgaatatt gtcagaaaaa
    21061 tgagcattta atcctgattc agacaatttg tctgctaatt tttttgcagc tgcttctttt
    21121 ccatcaccta aattttgtaa ttctactgaa ccacttccaa ataatttgac ctgaccagga
    21181 ccttcaagac ctgccgtcca catggtattt ccaatataga tattttccat tggtacaaat
    21241 ttctcaataa tatcttcatg cccgatacca tttaatagac ataagacctc tgtatctttt
    21301 ttgattaatg attggatatc ctgcagcatt ttttccagct gcatcgcttt ggtaaataga
    21361 ataatcagat caacttgatc ttctttttct acttcggatt gaagaacgat tggtagtttt
    21421 gcttctacct ctttaccatt aaaatttgct tgtaatccat gttctttgat ttgttggaca
    21481 tgttctgccc agccatcgat taataatact tcgttcccac tttggtgaag catcaaccca
    21541 aagcgacttc ccattgcgcc agcgcctgca attgctattt tcattgattc ttcctccttt
    21601 ttctttctat tatactcatt tgagaatctt ttcacaacct cgttttgtta ttcttctctt
    21661 atttatcatg tttatatcca ccattcaagt tattttatca gcaaatcaat aaaaactgga
    21721 atagaatccc aatttcatcg taagagaaac tagactagca ataagaaaat agaaacattc
    21781 atagaaaaaa tctcactcct ctgattataa aagagggggc attaacctcg tgtatactaa
    21841 aaccttccat tcattcgatg gatgattttc tttttgtgaa aaaatattca ttgtagtacg
    21901 atgttgcgca acacagacgg atggtattgc ttattattta ttaaaatcga acaatacact
    21961 tatttaaaaa cttctttccc agattttctt ccatttttac agttataact agtattatca
    22021 tgaaaaatag ccttttattg tctatgggaa taataattta aaattggtac ttttttatta
    22081 attagttcat cgcccaataa tctctccagt ttcttagcat ctcctgactc aaccggaatt
    22141 atccaagccc agtctaaata attttttaaa atctcatcat attttgcaaa tatttcattg
    22201 ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttat attgagttga
    22261 taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac agtaatgacc
    22321 attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc acctaatgaa
    22381 tcaattattt cttgcccttt agctctaata tattgatctc tattacctat ctcactctta
    22441 tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt aaattcgctt
    22501 ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa ctctgattta
    22561 ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc aaaattgagc
    22621 ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt atattcatca
    22681 ttcataaaac caatccagct cctcgattaa gataatattt taatctcata caaaaactag
    22741 ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac ttttctccga
    22801 ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca aggcttaaaa
    22861 tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg atagatttct
    22921 gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat ttttaatttt
    22981 tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt gtctactatc
    23041 ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa tgcgtcaaaa
    23101 acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt gttcttatcc
    23161 tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc gcctagctca
    23221 aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg caataaccta
    23281 tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac gtactttttg
    23341 tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata aggcgctggg
    23401 tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag ttgatcggac
    23461 ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta acatcttttg
    23521 ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg acaatgtatt
    23581 cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt ctttgtactt
    23641 tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata tctggtgaaa
    23701 gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt tcccagaacg
    23761 gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata gcgggtccag
    23821 atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa gtatctcctg
    23881 aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca acaaaaatcc
    23941 catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca acactggata
    24001 aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca tcggttgtcc
    24061 aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa tttttccagg
    24121 cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg atatcaatcg
    24181 gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta aagaaatcaa
    24241 ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt tctaaaacct
    24301 ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct attgagccat
    24361 acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc taatagtttt
    24421 tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat atcgtcttga
    24481 cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg taaattttct
    24541 ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg taaagcatca
    24601 tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata atcccgggtg
    24661 tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt ttgagcatat
    24721 gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata atcttctgct
    24781 cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt aaattctcct
    24841 tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac ataataccca
    24901 gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat gggttcacgg
    24961 ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc ataattaaat
    25021 gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat cttagctttt
    25081 tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac tacgattggt
    25141 aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc taatggactt
    25201 tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc acgtaagacg
    25261 aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa aattccaggg
    25321 ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga aggtgaccct
    25381 tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc accattttta
    25441 ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt atttaaacaa
    25501 ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat agatgatttg
    25561 ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta actgcaaagc
    25621 gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt cagaaatata
    25681 ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa ttatttcgtc
    25741 tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt cgacatatgc
    25801 acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct ctttaaaacc
    25861 ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa tccttttcct
    25921 ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga gtagaatcaa
    25981 aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag taataattac
    26041 tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac taacttcacc
    26101 aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg atccaactac
    26161 tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac cgtattcagt
    26221 aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga aaaagaagat
    26281 taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta aagccatata
    26341 agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt ccccaatacc
    26401 agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg cttctaatcc
    26461 ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc aaggctgtgt
    26521 attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat ctttgtataa
    26581 ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg tttgataatt
    26641 aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct ttttcgttac
    26701 ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca gaagctcccg
    26761 cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat gctaaagcga
    26821 atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag aaaaatccaa
    26881 caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc gcaataccaa
    26941 cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta ggtaaaacat
    27001 ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact ggaattgctg
    27061 cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc ttaaattctc
    27121 gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt ttgacaatta
    27181 catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg gcttctactc
    27241 caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc ggtacagatg
    27301 agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca actatcaaac
    27361 ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact gcgtaattta
    27421 caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat atagtcagca
    27481 taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa ttttgattga
    27541 atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc agaatccaac
    27601 aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga tagctgtaat
    27661 gcattatctt taacaacagt accgccaaca attagtcggt caattctaat accgttatct
    27721 actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc ctgatcgtat
    27781 ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac agccattcca
    27841 gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac agaatcatcg
    27901 atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat aatcccatgc
    27961 aataaacggt tatcaactct cgacataaca atactcataa atacttcctc ctttatcttt
    28021 cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat caagctcttt
    28081 ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta taataatgtt
    28141 caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc tcactcgttt
    28201 cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca attaagcaac
    28261 taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct agatcatttt
    28321 gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcggattt aatatggtca
    28381 attgctccat taaattcgtt agactaaaat tcttcagaat attattgttc aaaactttca
    28441 gttcattatc attaaagtgt ctattcaatt gattggagaa tttacttata ttcattccta
    28501 aaatcatatc ttcaattgaa aagaattcta ca
//
LOCUS       DAZBTH010000023        28383 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000023 DAZBTH010000000
VERSION     DAZBTH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.023"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..1020)
                     /locus_tag="ACHGNW_001029"
     CDS             complement(325..1020)
                     /locus_tag="ACHGNW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN6925359.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1110..2210)
                     /gene="ychF"
                     /locus_tag="ACHGNW_001030"
     CDS             complement(1110..2210)
                     /gene="ychF"
                     /locus_tag="ACHGNW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN6925360.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2219..2410)
                     /locus_tag="ACHGNW_001031"
     CDS             complement(2219..2410)
                     /locus_tag="ACHGNW_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN6925361.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2431..3321)
                     /locus_tag="ACHGNW_001032"
     CDS             complement(2431..3321)
                     /locus_tag="ACHGNW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN6925362.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3308..4075)
                     /locus_tag="ACHGNW_001033"
     CDS             complement(3308..4075)
                     /locus_tag="ACHGNW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6925363.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4087..4800)
                     /gene="rsmG"
                     /locus_tag="ACHGNW_001034"
     CDS             complement(4087..4800)
                     /gene="rsmG"
                     /locus_tag="ACHGNW_001034"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN6925364.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5032..6933)
                     /gene="mnmG"
                     /locus_tag="ACHGNW_001035"
     CDS             complement(5032..6933)
                     /gene="mnmG"
                     /locus_tag="ACHGNW_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN6925365.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6950..8347)
                     /gene="mnmE"
                     /locus_tag="ACHGNW_001036"
     CDS             complement(6950..8347)
                     /gene="mnmE"
                     /locus_tag="ACHGNW_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN6925366.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8646..9029
                     /locus_tag="ACHGNW_001037"
     CDS             8646..9029
                     /locus_tag="ACHGNW_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925367.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9197..9955)
                     /gene="jag"
                     /locus_tag="ACHGNW_001038"
     CDS             complement(9197..9955)
                     /gene="jag"
                     /locus_tag="ACHGNW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN6925368.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9973..10791)
                     /locus_tag="ACHGNW_001039"
     CDS             complement(9973..10791)
                     /locus_tag="ACHGNW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN6925369.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10788..11144)
                     /gene="rnpA"
                     /locus_tag="ACHGNW_001040"
     CDS             complement(10788..11144)
                     /gene="rnpA"
                     /locus_tag="ACHGNW_001040"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN6925370.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11262..11396)
                     /gene="rpmH"
                     /locus_tag="ACHGNW_001041"
     CDS             complement(11262..11396)
                     /gene="rpmH"
                     /locus_tag="ACHGNW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN6925371.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11919..13253
                     /gene="dnaA"
                     /locus_tag="ACHGNW_001042"
     CDS             11919..13253
                     /gene="dnaA"
                     /locus_tag="ACHGNW_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN6925372.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13451..14581
                     /gene="dnaN"
                     /locus_tag="ACHGNW_001043"
     CDS             13451..14581
                     /gene="dnaN"
                     /locus_tag="ACHGNW_001043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN6925373.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14810..15052
                     /gene="yaaA"
                     /locus_tag="ACHGNW_001044"
     CDS             14810..15052
                     /gene="yaaA"
                     /locus_tag="ACHGNW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN6925374.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            15039..16163
                     /gene="recF"
                     /locus_tag="ACHGNW_001045"
     CDS             15039..16163
                     /gene="recF"
                     /locus_tag="ACHGNW_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN6925375.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            16160..18106
                     /gene="gyrB"
                     /locus_tag="ACHGNW_001046"
     CDS             16160..18106
                     /gene="gyrB"
                     /locus_tag="ACHGNW_001046"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN6925376.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            18126..20597
                     /gene="gyrA"
                     /locus_tag="ACHGNW_001047"
     CDS             18126..20597
                     /gene="gyrA"
                     /locus_tag="ACHGNW_001047"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN6925377.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20833..21132
                     /gene="rpsF"
                     /locus_tag="ACHGNW_001048"
     CDS             20833..21132
                     /gene="rpsF"
                     /locus_tag="ACHGNW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN6925378.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21182..21694
                     /gene="ssb"
                     /locus_tag="ACHGNW_001049"
     CDS             21182..21694
                     /gene="ssb"
                     /locus_tag="ACHGNW_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6925379.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21720..21956
                     /gene="rpsR"
                     /locus_tag="ACHGNW_001050"
     CDS             21720..21956
                     /gene="rpsR"
                     /locus_tag="ACHGNW_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN6925380.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            22099..24072
                     /locus_tag="ACHGNW_001051"
     CDS             22099..24072
                     /locus_tag="ACHGNW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN6925381.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            24078..24530
                     /gene="rplI"
                     /locus_tag="ACHGNW_001052"
     CDS             24078..24530
                     /gene="rplI"
                     /locus_tag="ACHGNW_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN6925382.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24804..26171
                     /gene="dnaB"
                     /locus_tag="ACHGNW_001053"
     CDS             24804..26171
                     /gene="dnaB"
                     /locus_tag="ACHGNW_001053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN6925383.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26951..28243
                     /locus_tag="ACHGNW_001054"
     CDS             26951..28243
                     /locus_tag="ACHGNW_001054"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN6925384.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8648 a         5556 c         5641 g         8538 t
ORIGIN      
        1 caatcaaaaa cagcttctca tgtttttgaa ttggttgggt ttattaccgc agatgcaaac
       61 ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc ttagtttcga gtagtaagaa
      121 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
      181 ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca agacttttgt cacagtccct
      241 tactattatt taactatgga taaacaaaat gaggttcttt actaccccta gatatctagc
      301 aaaatagctg atctttttgc tagtttatct tcttgggacg gataaacttc caataatccc
      361 gtattttttc ttcaagtagt aacggattgc taaagcagtt gccccgataa tgataactac
      421 tactggatct aatactggat tgataactgg tggcaatagt gttgtcgcag aaaacagtac
      481 gacccaaaga agtgtcgttc cacctaggat aagcaatgtc ttacccattc ctggtttctt
      541 gcttcgatct gctccgggat attcatattg atagatgtat ttgtacatca tataaaagac
      601 ccagccaccg attgcagaac ctagaacgag tgtagaaaag ccataagctg gcgttgttcc
      661 acgtgaaaac aatccggtaa tcgcaatcat gatacttaat agaccaagca acagcaacgt
      721 attatctaac cacatcaata aaggggtgga tgaatttttt acttctgctg gcttattgat
      781 gattgcttcc gttctttcag agactgtccc aaacaattga cgggctgttc ttccgccttt
      841 ttgttctgca actaaagtag gcagaatgtc atgaagggct tttacttttt cttcttcaga
      901 caaattcgct gcttctaaag attttttcaa atcaaaaatg tactgttgat ttcttttggt
      961 taattttgtt tctaaactat tgttttcttt tacgattgct cgtaattgtt ctggttccat
     1021 tatctaatag tcctccttag ccgttccttt tctcaacggt cgtcacaaaa gacattctac
     1081 catattctac agctagtttg atagcccttt tacacattaa agcggaataa cattacgtcg
     1141 ccgtcttgta cgacataatc ttttccttct aatcgaacac gtccagcttc ttttgctgca
     1201 tgcatgtttc cgtaatgatc caagtcttca aaagagacag tttctgcacg gataaatccg
     1261 cgttcgaaat ctgaatggat gattcctgct gcttgtggtg ctttgattcc ttttttgaat
     1321 gtccaagcac gtacttcttg ttccccagca gtaaagtaag tagctaagcc taacaaatca
     1381 taagcagcac ggatcaactg atccaaacct gattcttcga ttcctaaagc ttctaaaaat
     1441 tccgctttgt cttcttcatc taattctgcg atttcttctt ctgcacgagc actgaccacg
     1501 atcacttctg cattttcttc tgcagcaaag ttgcgcactt cttgtacata atgattcgcg
     1561 tctgcatctg ctacgtcatc ttctgagaca ttggctacat aaagaattgg ttttgtcgtc
     1621 aataaaaata gtgatttaac gattttttgt tcttcttcag taaattcgat cgttcgggca
     1681 gattttcctt cttccagtac aggtttgatt ttatccagta cagctagttc tgccactgca
     1741 tctttatctt tggttttagc aacttttgct acacgagtat agcgtttgtt gatagattct
     1801 aagtctgcta acactagttc caagttgatc gtatcaatat cttctagtgg atcaacacgt
     1861 ccttctacgt gagtaatatt atcatcatca aaacagcgaa caacgtgaca aatagcgtct
     1921 acttggcgga tatggcttaa gaattgattt cccaaacctt ctcctttact tgcgcctttt
     1981 acgattccag caatatctgt aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca
     2041 agttctgtta agcgttgcaa acgccagtca ggtacttcta ccatccctac gttggggtcg
     2101 atcgtcgcaa agggatagtt tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa
     2161 gttgattttc ctacgttggg caacccaaca attccagcag tcaatgccat aaatttattc
     2221 actctttctc tgcatttttt tcaattactt tttttaattt cttttcaaaa tctcgacggg
     2281 tcatcatgac gatatgtccg caattcatac atctgatttt gatatctgcg cccattcgaa
     2341 tgacttccca gcgattcgtc tggcaagcgt gaggtttttt catttcaacg atatcgtgca
     2401 agtcgtacat aaaaatagcc tcctttttgt ttattcttca tcaaattgaa tattcaagat
     2461 atcaagaatt cgagtcaaat ctttttgaga aagatattca atctcgatct tacctttccc
     2521 atttttttct tggatagcta cacttgtacc aaatttatcc atcaaacgat cttcgctttc
     2581 tcggatataa tatggttttt cctttgctag ccttgggatt tttttctttt cttttccatc
     2641 attttgattt aactcagaaa caagctgttc cagttggcgg acagttaaat tttcagttac
     2701 tgcacgattg gctaatttca agatcaatgt tttatttttt aaacctaaca gcgtacgggc
     2761 ttgtcccata gataaacgct gatcttgtac catttccttg acaagatccg gtaaagaaag
     2821 caagcgcaaa taattggcga tataaggtct gctctttcct aaacgactag ctacttctgc
     2881 ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct tctgcttctt ctaacggatt
     2941 cagatcttct cgctgcaagt tttccagcac tgctacttgc atcatcgctt cttcgtcaaa
     3001 gtctctaacg atagcaggga tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg
     3061 ctcccctgca ataatctcat atccttttac tgcggaacgg cgtacgatga tgggttgaaa
     3121 gacacctgat tgttcaatag aattagctag ttcctgcaaa gatacttctt caaatgtttt
     3181 acggggctga tacggatttg gtcgcagctc attcaatggt agctggacaa cggtatcatc
     3241 ttttacatcc accgtctcta agttttctaa atcttgaaac agtgcatcga ttcctcggcc
     3301 taatccttta cttttcttca cgacttacca cttcctttgc caatgcttgg tagacttcag
     3361 ctcctcgaga acgtggatcg tagtcgatga tcggttttcc atgactaggt gcttctgata
     3421 aacgaatatt tcttggaatg atcgtttcgt aaactttttc gcggaagtat ttacgtactt
     3481 cttctacgac ttcatttccc agatttgttc gtgcatcata cattgtcagt aagactccct
     3541 cgatctctaa ttctggatta aagtgttttt ggactaaacg aaccgtattc agtaattggc
     3601 ttaatccttc caatgcgtaa tactcacact gaacaggtat caaaatcgaa tcacttgctg
     3661 taaatgaatt gattgtcaga tggccaagag aaggcgggca atcgatcaaa atataatcat
     3721 actgactgct tacttctgct aaagatcctt ttaatcttga ttctcttgcc atcattgacg
     3781 tcaattcgat ctctgctcct gctaattgga gagtcgcagg cacaatactc aaattttcgt
     3841 gttcagtgat caaagttgcc tcatcaattg gcaattcatt gactagaaca tcatagatgt
     3901 ctcttgtcac atcaggtttt ctgatcccga caccactggt agcatttcct tgtgcatcca
     3961 tatccactag caatactttt ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg
     4021 tcgttgtttt gccgacgcca cctttttggt tcgcaacaga tattatttgt gccatctctt
     4081 ctcctcctat ttgattggct gcttattagg cagtccgggc tttcttgggt atttctttgg
     4141 ggttggtttc tttttttgaa taacgatgat atgccgttga tcagagacat tcggcaaggt
     4201 aaccgcctct tctttgagga atttaccacc taataaagca atcgcttgtt ttgcttctgt
     4261 caattcctct tcactttttg ctgctttcaa tgcataaaaa tagccttctt gtttagccaa
     4321 gggcaaacac aattccgcta acacattcaa acgagcaacc gcacgagcag tgacaaggtc
     4381 aaatgatgca cgaaagtgag gattttgccc aaatgtttct gcccggtcat gatataagga
     4441 agcatcgatt cctaataatc gggttaactc agataagaag gttattcgtt tattcagaga
     4501 atcaacgatc gttatctgca gttctggaaa ggcaattttt aaaggtatgc taggaaaccc
     4561 tgcccccgaa ccaacatcgc ataaagataa aggttgcgta aagtctactt caaatgctaa
     4621 tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt tcatccgtaa tagctgtaag
     4681 gttcatcttt tcgttccatt cgactaatgt ccgataataa atagcgaatt gattcatttg
     4741 atgatcggta agctctatcc ctttttctgc caaggattgg cgaaattctt ctggtatcat
     4801 aaatattcct ttcattgtga aacagcaatt gtttcacaga ttctctccct actttaacgc
     4861 aaaatctcta gacatgcaat ccttccatat ggattcttaa aaatttgaaa agtaagaaaa
     4921 gccgatagac tttttctttc tagtatgact cataaaatat acacagaaga gagaactagc
     4981 tttgagcaat ttctgcaaaa actagttctc tcttctattt attattacgt attacgctgg
     5041 ttcactgact ttcgcaatct tcccttgttc gatatagacc attaggatac ttaggtctgc
     5101 agggtttacg ccactgattc ggctagcttg ggcaattgtt tctggttgga ttttctgcaa
     5161 tttttgtttt gcttctgttg ctaatccgtt gattgctgcg tagtcgatgt tttctggaat
     5221 ctttttcgct tccatgcgtt ttaatttttc tactttttcc atggcttttt tgatgtagcc
     5281 ttcatatttg atttggattt ctacttgttc gatttctttt cgtgtcaatt cttcattttc
     5341 tggaataaac cgtagaagtt ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc
     5401 tagtacacca tcttttaatg gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc
     5461 tgttggtttg attcgtacat tcatcaaacg tttgatttct tgttcaactg cttctttttt
     5521 cttcaaataa ttcttgtatt gatcttcttt tactaagcct acacggtgac ccatttctgt
     5581 taatcgcaaa tctgcattat catgacgtaa aagcagacgg tattcagcac gtgaagtcag
     5641 caaacgataa ggttcgttcg ttccttttgt taccaaatca tcgatcatga caccgatgta
     5701 accgtcactt cgtttgatca ctaaaggttc ttttccctga actttcaaag ctgcattgat
     5761 accggcaatc agcccttgtc cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc
     5821 agctgtataa agattttcaa cgactttagt ttctaatgtc ggacggagct ggtgtggctc
     5881 tactacgtca tattcgatag catatcctgt acgcatcatt tctacttttt ctaatccagc
     5941 aatagaatga agcatgttga cttgaacatc ctctggtaaa gaagtagata aaccttgaac
     6001 atagacttct tccgtattta agccttctgg ttccaaaaat aattgatgac gtggtttgtc
     6061 tgcaaaacgc acgatcttat cttcgatgga tggacaatag cgagcgccta ccccttcaac
     6121 gatccctgta aacattgggg cacgatgtaa gttgtctcga ataatttggt gagtcgtctc
     6181 acctgtatag gtcagccagc aagattcctg atctagttta taggcactat ctggtgtgct
     6241 gaagctaaaa tgattcggct ctttgtcacc aggctgctct tctgtaacag aatagtcgat
     6301 cgtacttgat tttactcgag gcggtgttcc tgttttgaac cgttcgatag caaagccaag
     6361 ttcttttaga tgattcgcta gtccaataga aggttgagag ttattcggac ctgatgaata
     6421 tttcaattca ccgataatga tttctccgcg aagtgctgtt ccggctgtaa taacaactgc
     6481 tttggcccgg tagctcgctc ctgtggacgt cactactcca tgacaaacac catcttcgac
     6541 gatcagttct tcgacgattc cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg
     6601 tttcatttct gttgaataag catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg
     6661 gccttttcct gtgttcaaca tacgcatttg gatataggtt ttatcgatat tcttacccat
     6721 ttctccgcct aacgcatcga tttctcgaac gacaacgcct ttagctggtc cgcctacaga
     6781 aggattacat ggcatgaatg ctaccatgtc caaatttatt gtcagtaata atgtttttga
     6841 tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat
     6901 cacgtcatac gcatctgctt cataatgttc caatcgtttg ttcctccttt tattttccta
     6961 aacaaaattg actgaataat tgagtgatta gctcatcttg tacactgtcg ccgacaactt
     7021 ctcctagata gtcccagcaa cgcgtcatat ctatttgaac gagatccaca ggcattcctg
     7081 aatcaatacc tgcaatgact tcttcaagtg agagggaagc tttttctaac aacgcgatat
     7141 ggcgggtatt agaaacataa gtagcgtctc tttctcctgt ttctccgctg aagaataaat
     7201 cagaaatagc tgattccaaa cgatctagtc cgtcgttttt agctacggaa attgaaaaaa
     7261 ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg
     7321 ttttatttag aagaatgatc cgtttcaatc cacttgtcgc ttccaataat tgttcatctt
     7381 ctgctgtcag tggttcgctt tggtttagaa caagtaagat caagtctgct tctgctaatg
     7441 cttttcggct tctttcgacc ccgatccgtt ccaccacatc ttctgtttcc cgaattccag
     7501 ctgtatcgat tagcttgagc ggtacccccc gaacattgac atactcttcg attacatctc
     7561 gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc tcttaacaaa tggtttaaca
     7621 aacttgattt gccgacattt ggccggccga tgattgccgt acttaaccct tcccgcagaa
     7681 ccttcccttg cttggatgta gcaagtagcg cttggattct ttgttggatc atctgcgctt
     7741 tttcaagcaa taacttcgta gtcaattctt cgacatcatc atattctgga taatcaatat
     7801 ttacttctac ttgtgctaat gtctctaaaa tctcttgtct caatgagcgg atcaaagcag
     7861 acagattgcc gtctaactga ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa
     7921 tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt
     7981 ttgtgaattc gcctggctca gctaatcttg caccttctct taacaatagc tgcaaaattt
     8041 gattgactac aacgatccca ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg
     8101 tctttggtgc tctcatcacg gagaccatga cttcatcgac aagctgattg ctcttgggat
     8161 caacgatatg tccataatgg atcgtatgac ttggtacttc tgataatcgt ttgtttccag
     8221 attgataaac tttatctgct agttgcacag cttgatcgcc acttaaacga acgatactga
     8281 ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt atcaaattct aaagtaattt
     8341 cagccatttt ttcacttcct ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt
     8401 tatgcacaat aaacggtgaa cttcgcactt tgactgcttg ttcttgatta aattctggtc
     8461 atagattatc acacttgcta taaggaatca agtgtttaaa acaattagat gaagttgagc
     8521 ctgttctgct tccattacct tatatttaat tgtccttgga tgtctctttg cgttctttgg
     8581 tttattcgaa gagaagatac tgtataaaca aggctttatc tttcttaaaa aaaacggtac
     8641 actaaatggt agcgctatct aaactataca cttttgaaag gagaaaatat gtgagcccgt
     8701 tcagatctgc tgcattggaa gaagaaagtg accctcggat caacgatgtc gttttatcta
     8761 cagggaatat caaccagaaa tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag
     8821 aagctgattt gtttgaccca tcttttccca aagatgacct tttcttaaca acgaagagga
     8881 aattaaagac gaaggtactt gaatatggtg cgaatgcagt aatcaactgt cattttgagt
     8941 atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact ttttgcttat ggaacagtga
     9001 tccaatttat ccaaacaacg attggttaaa tgaaacagac aaaaaaagcg gatcaaaact
     9061 gaagacagtt tcgatccgct tttttctatc aaagttagtg actacttcat cgtatactct
     9121 gataagaatg atttctagta aaatatactt ggtttgaaaa actttgtcaa cgtgatttat
     9181 tcaaaaagta atggaatcaa taatattttt tagcaggttc tactactaaa taacgataag
     9241 gttcctcacc ttccgaatgt gtttttacat gttcatcttt gcttaatgca gcatggattt
     9301 gtttacgttc aaatgcaggc attggttcta agaaaacagg ctgccctgtc cgttttactt
     9361 tttcagccgt gcgatcagct aaacgctgaa tgatctcttg gcgcttttca cggtaattgc
     9421 ctacattgac tactacagac agcttatttc ttgctaaacg atggatgaag acttgtgcca
     9481 gatattgcaa tgcattcagt gtttttccat gtttcccaat cagtatgcct tgcttttgtg
     9541 tttctaaatg gaaaacaacc atattatttg attctctttc cattttgaca agagctggag
     9601 catcaagctg ttttgaaatt tcagtaagat aaactgccaa ttccgttaaa gctgcttcat
     9661 cacctagctc acttacttct tctgtttttt cttcaagaat cgtttcgttg actacctcga
     9721 tctcagctgt tggttcttct tccaaaattt cttctacagt ctgttctact gtttcagaaa
     9781 ctttttcgct gatacttggc tggatcgaaa gccgtgcatt ctttcgaccc attcctagga
     9841 aacctttttt tccttcttcc agtatttcga tttcgacttg gtctttggcc aaacccaaat
     9901 cagccagacc tagttggaca gcttcgtcta ttgttgcagc ttcataaatc ggcattagca
     9961 ttacctcccc tattatttcc gttttttctt aggattcatt gctttcttaa gagcacgttc
    10021 tctttcgcgg attctccgtg cttcttcttc acgctctttt cgaattttga aagggttgtt
    10081 gatcagcagc gtttgtacaa cttggaaggc attggaaact acccagtata atgaaagacc
    10141 gctggctaaa ttgacaccca tcactaaaat cattacaggc atcacgaaat tcatgatctt
    10201 cattgatgca ttcgattcaa tttgactcat gctagataaa tacgtacttg caaaagtaaa
    10261 gattgctgct aaaatcggca aaataaatgt tggatccgcg ttccctaagt tcaaccataa
    10321 gaaatgccct tgtttcaact ctggtacacg tgaaatagat tgccataacg ccatcaagat
    10381 tggcatttgt actaataaag gaagacaacc agcatatgga ttgacgccat tttctgcata
    10441 taaacgctgc tgctcttctc ttaacttact ttgtgtttcc ggatctcttg aactatattg
    10501 ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg
    10561 gaaatgcatc aacggcaata ggatcacgcg gatgatcagt gtaaataaaa tgatccctat
    10621 ccctgcatta ccaaatgaca acgccttgat cgcctcagca aagtaataaa caatataacg
    10681 atcccaaatt cctgtactct gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat
    10741 gaccaaacta acgataccgg ccatcaaaag taaacgtttg tatttcttca cttactcttt
    10801 tcctctctgt cgattatttt tgcaagtttt aagacgtgga ctaaattgga gcgaacctca
    10861 tcataagtca accccttcac acttggtcta gcaatcacaa taaaatccaa ctccggatca
    10921 atttcttctt ttatagaata aactgctgct cggatttttc gtttgacttc attgcggttt
    10981 accgcgttgc cgaccttttt tcctacagat aaaccaactc gaaaatgagg ctgtcccggt
    11041 ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag aatctcccgt atggaacacg
    11101 gcttgaaact ctcgttcttt ttttactcga tacgcttttc tcattagttt tctttccttt
    11161 tctatctctc acttacgctc ataatcatac cataattacg attgtccttt catcatttcc
    11221 ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg cctaagctga aagaactttt
    11281 cttcctttgc gacgacggct agctaacaca cgacgtccat ttttagtact catacgttta
    11341 cggaatccgt gaactttttg acgtttacgt ttatttggtt ggtatgttct tttcatatat
    11401 tccacctcca aaatgttctt atctatttta taaacacaga catactttga tagtataacg
    11461 acaaagtagt gattttgtca atataaaata gaagatcgat tgttaaaact tatccacaag
    11521 attatttttt tctatgggaa tcctatgtgg aaaaagttat gcacagggat tatccacaga
    11581 taataaaagg ctggttttac acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg
    11641 cgatttcctg tgtattccgt ctcctaacta ttggtatatc tgtatttttt atccacagac
    11701 gcaagaggaa atctattggt ttttacgagt tttccacctg tgcattagtg gtgtggaaaa
    11761 ctttttttga caactgtgtt tttctctttt ttctttttgt cgattgtgtg gataattgta
    11821 attaaaaaat ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt
    11881 actagttatt cactttttaa tgaaggagga ggccttatat ggtatccctc gatgctttat
    11941 ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc tgctagctat aatacatgga
    12001 tcgaaacagc gaaccctcga acccttgatc aaaaccagct cgttgttgag gttcctagca
    12061 agattcataa agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata
    12121 tgttatcagg aaatgagatt atccctcgct ttattactgg cgaagaagca gagcaagaag
    12181 aagttataga agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa aaagccatgc
    12241 tgaaccctaa atataccttt gatacgtttg tcattggtaa aggaaaccag atggctcatg
    12301 cagcagcgct cgttgtagca gaagatccag gttctattta taacccgctc ttcttttatg
    12361 gaggcgtggg acttggtaag actcacttga tgcatgcaat tggtcatcaa atgctgcaaa
    12421 gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat gatttcatca
    12481 attcgatcca aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat gttgatttac
    12541 tgctagtgga tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc
    12601 atactttcaa tgccttgtac aatgaaggga aacaaatcgt actaaccagt gatcggttgc
    12661 caaacgagat tccgaaactg caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg
    12721 ttgatattac accgccggat cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg
    12781 ctgaacgtct agagatccca gatgacacac taagctacat cgcaggacaa attgattcta
    12841 acattcgtga attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg
    12901 aagatatttc gactagtcta gctgcagacg cactgaaaac gttaaaatca ggaaaaggac
    12961 atcctcaatt gtcaatcctg cagatccaag aagaagtggc aaagtattat cacatccaat
    13021 tgaaagatct caaaggaaag aaacgcgtca aatctattgt tgttcctaga caaatcgcta
    13081 tgtatttggc acgtgagctc acagataatt ctttaccgaa aatcggagct gaattcggtg
    13141 gcaaggatca tacaacggtt atccatgctc atgaaaaaat ccagcagctg atggattcta
    13201 gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat taatttgtcg
    13261 attctatcaa ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa gttatacaca
    13321 ctttttccac agctgttttt tcgtttctct cttacgtttt ttgagttttc cacagaatca
    13381 acatgcccta ttacttttat tattcttttt aactaataat aaataaacaa acattcaaag
    13441 gagatacatc atgaaagtta ctttaaaccg agctagcttt atgcaggaat tgcaaactgt
    13501 tcaacgagct atttcaagca aaaccacgat ccctattttg acaggtgtaa aaatcacact
    13561 gacacaagaa ggtttgactt tgacggggag caacgctgat atatcaattg aaactttttt
    13621 gtctgttgaa aacgaaaaag caaatatgca aatcgaatct actggttcca ttgttttaca
    13681 agcacgtttc tttagcgaaa tcattcggag acttcctgaa gaaacattta ctttagaagt
    13741 tttagaaaat aaacaagtag cgatcacttc tggaaaagcg aattttatcg taaatggatt
    13801 agatgcagat aactatcctc atcttcctgt tgtcgaaagc cataaccaga tgaaattacc
    13861 tgtacacgta ttgactaaac taatcaacga aacagttttt gctgtctcac aacatgagag
    13921 tcgtccaatc ttgacaggtg tgcatttcat tttatctgat aattctttat tagctgtagc
    13981 tactgattct caccgtctaa gtcaacgcgt gattccagta gaacaagcgg ctgatcattt
    14041 tgatattgtt attcctggaa aaagtttgat cgaattatct cgctcattaa caaatgaaga
    14101 agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta
    14161 tttctattcc cgtttgctag aaggaaacta tccagatacc aatcgtttga ttccttcaag
    14221 ttttaataca gaggttgaat tttctgttcc tagcttttta gcggcgatcg aacgggcttc
    14281 tttactttct catgaaggcc gtaacaacat cgttcgttta tctattcgtc cagatgcggt
    14341 tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga gttatacagc
    14401 aagcagtggc gatccattag atatttcttt caatccggat tatatgaaag cagcgttgcg
    14461 tgcatttgga gatatgagca tcaaagtgaa atttatctct gctattcgtc catttacatt
    14521 ggaaccaacg gaagatggcg ttcaatttat tcagctgatt actcctgtac gtacaaatta
    14581 acaatgattt tcaatgttct tgaaaattta attgaaaaga aatagaaaaa ataaaatttt
    14641 aaacagaagg ccctaaaatc tcttttaagg ctttctgttt atttttgttt aaatcacctt
    14701 ttttagatta aatcgtctta tatggaaaaa tatatggata aatttgtttt attgcgaaaa
    14761 aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat tgaaacaaca
    14821 agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat
    14881 cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga
    14941 accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt
    15001 aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt
    15061 taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct
    15121 ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta
    15181 tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg
    15241 cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa atcttattat
    15301 ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta agtacttacg
    15361 tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc
    15421 cgcagaccag gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt
    15481 atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg aagcaattga
    15541 atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt
    15601 ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta
    15661 attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg
    15721 cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt
    15781 tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg
    15841 acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc
    15901 aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacag
    15961 gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat aatcgtcaac
    16021 tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg
    16081 accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac
    16141 taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta
    16201 tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat
    16261 gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa
    16321 ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga
    16381 caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac
    16441 tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg
    16501 tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg ttaacgcgct
    16561 ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg
    16621 tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac
    16681 ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa
    16741 attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga
    16801 agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggattaaaag
    16861 ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga
    16921 aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc
    16981 caatatcatg agttttgcca acaatatcca tacgtatgaa ggcggaacac atgaatcagg
    17041 attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa
    17101 agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc
    17161 tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga
    17221 agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa
    17281 cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc
    17341 agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc
    17401 aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga
    17461 aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt
    17521 gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa
    17581 tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt
    17641 atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca
    17701 tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg
    17761 atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta
    17821 tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc
    17881 ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact
    17941 atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc
    18001 tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc
    18061 attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa
    18121 aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga
    18181 aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca
    18241 gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa
    18301 caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc
    18361 atcctcatgg agactatgcg atctatgagt caatggtacg gatggcacag ccattcagct
    18421 accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg
    18481 ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata
    18541 tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg
    18601 tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta
    18661 tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga
    18721 tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc
    18781 caacaggtgg tttggttatg ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg
    18841 gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga
    18901 tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg
    18961 aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg
    19021 aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt atcttgaata
    19081 acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa
    19141 aaggtgtgcc gaaagttttg agcttgaaac aaattcttga aaactatatc gaacatcagc
    19201 gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa agcagaagca agggcacaca
    19261 ttttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg
    19321 ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga acggttcgat ttatccgatc
    19381 gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa
    19441 aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg
    19501 caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat
    19561 ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag
    19621 atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt
    19681 tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta
    19741 tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat
    19801 tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc
    19861 gcacagccaa aggaatacca attatcaatt tactaggtat cgattcgagt gaaagcatcc
    19921 aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc
    19981 gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc
    20041 tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc
    20101 aatctaatat cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag
    20161 tccgcgatat gggacgtaca gcttccgggg ttagaggaat acgcctgcgt gaggaagatt
    20221 atgtcgttgg ggcagcctta atgaaggaag atcaagaagt attagtgatc acagaaaaag
    20281 gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga
    20341 tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg
    20401 gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga
    20461 ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata
    20521 cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac
    20581 aaggatcaga agaatagcca tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa
    20641 aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa
    20701 actgttcatt ctgtattgct tttgaccaaa atagagtgta taatcagagc ttgtgagtag
    20761 tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg
    20821 aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt
    20881 gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga
    20941 gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt
    21001 taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc
    21061 aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa
    21121 gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat
    21181 tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac
    21241 agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca
    21301 aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac
    21361 tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg
    21421 ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt
    21481 ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag
    21541 ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc
    21601 atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac
    21661 gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa
    21721 tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc
    21781 atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg
    21841 gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg
    21901 ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa
    21961 tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa
    22021 aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc
    22081 atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc
    22141 cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt
    22201 agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa
    22261 cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc
    22321 aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc
    22381 caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca
    22441 gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat
    22501 ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat
    22561 gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt
    22621 attttatcta ttgataacta tgcagatgcc attgataaat tggatgacaa agagatttct
    22681 tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt
    22741 ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag
    22801 atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc
    22861 tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt
    22921 ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg
    22981 aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc
    23041 aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa
    23101 acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct
    23161 tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaacgag
    23221 aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta
    23281 gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc
    23341 gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata tgagcaattt
    23401 gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg
    23461 ctgacttata tcgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa
    23521 taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg
    23581 atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc
    23641 agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat
    23701 ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat
    23761 attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca
    23821 gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca
    23881 gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg
    23941 gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca
    24001 gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat
    24061 gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa
    24121 aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact
    24181 tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag
    24241 aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag
    24301 aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga
    24361 tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac
    24421 gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc
    24481 ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga
    24541 tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg
    24601 agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt
    24661 tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt
    24721 ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca
    24781 attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa
    24841 atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg
    24901 atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg
    24961 agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc
    25021 ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg
    25081 cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat
    25141 cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag
    25201 gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa
    25261 aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata
    25321 tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc
    25381 tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag
    25441 cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc
    25501 gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt
    25561 gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat
    25621 ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata
    25681 caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag
    25741 gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga
    25801 accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga
    25861 aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac
    25921 gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag
    25981 ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg
    26041 agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag
    26101 gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag
    26161 aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat
    26221 gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt
    26281 gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc
    26341 ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa
    26401 tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa
    26461 ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc
    26521 ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct
    26581 tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct
    26641 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa
    26701 gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca
    26761 ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc
    26821 taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat
    26881 tttatttgaa atatgtatct attattgata caatggttca ggattaaata atatagaacg
    26941 aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg
    27001 aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga
    27061 caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc
    27121 tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa
    27181 atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg
    27241 tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga
    27301 agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat
    27361 ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga
    27421 agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc
    27481 agaaccactt gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa
    27541 acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt
    27601 gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt
    27661 tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa
    27721 aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt
    27781 tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg
    27841 tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc
    27901 taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt
    27961 ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct accactatcc
    28021 agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc
    28081 agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt
    28141 acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag
    28201 aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact
    28261 gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt
    28321 gaacactgtt tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc
    28381 gaa
//
LOCUS       DAZBTH010000024        27903 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000024 DAZBTH010000000
VERSION     DAZBTH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.024"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(409..1353)
                     /locus_tag="ACHGNW_001055"
     CDS             complement(409..1353)
                     /locus_tag="ACHGNW_001055"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6925385.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1649..2593)
                     /locus_tag="ACHGNW_001056"
     CDS             complement(1649..2593)
                     /locus_tag="ACHGNW_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6925386.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2813..4027
                     /locus_tag="ACHGNW_001057"
     CDS             2813..4027
                     /locus_tag="ACHGNW_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925387.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4049..6064
                     /locus_tag="ACHGNW_001058"
     CDS             4049..6064
                     /locus_tag="ACHGNW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN6925388.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6147..7520)
                     /locus_tag="ACHGNW_001059"
     CDS             complement(6147..7520)
                     /locus_tag="ACHGNW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6925389.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7546..7830)
                     /locus_tag="ACHGNW_001060"
     CDS             complement(7546..7830)
                     /locus_tag="ACHGNW_001060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6925390.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7832..9847)
                     /locus_tag="ACHGNW_001061"
     CDS             complement(7832..9847)
                     /locus_tag="ACHGNW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6925391.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10026..10331)
                     /locus_tag="ACHGNW_001062"
     CDS             complement(10026..10331)
                     /locus_tag="ACHGNW_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN6925392.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10420..11451)
                     /locus_tag="ACHGNW_001063"
     CDS             complement(10420..11451)
                     /locus_tag="ACHGNW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6925393.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11658..12533
                     /locus_tag="ACHGNW_001064"
     CDS             11658..12533
                     /locus_tag="ACHGNW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6925394.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12685..13527)
                     /locus_tag="ACHGNW_001065"
     CDS             complement(12685..13527)
                     /locus_tag="ACHGNW_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6925395.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13619..15496)
                     /locus_tag="ACHGNW_001066"
     CDS             complement(13619..15496)
                     /locus_tag="ACHGNW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6925396.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15493..16914)
                     /locus_tag="ACHGNW_001067"
     CDS             complement(15493..16914)
                     /locus_tag="ACHGNW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6925397.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16917..20306)
                     /locus_tag="ACHGNW_001068"
     CDS             complement(16917..20306)
                     /locus_tag="ACHGNW_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6925398.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20563..21954
                     /locus_tag="ACHGNW_001069"
     CDS             20563..21954
                     /locus_tag="ACHGNW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925399.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22027..22674)
                     /locus_tag="ACHGNW_001070"
     CDS             complement(22027..22674)
                     /locus_tag="ACHGNW_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6925400.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22682..23368)
                     /locus_tag="ACHGNW_001071"
     CDS             complement(22682..23368)
                     /locus_tag="ACHGNW_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925401.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23374..24447)
                     /locus_tag="ACHGNW_001072"
     CDS             complement(23374..24447)
                     /locus_tag="ACHGNW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925402.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24669..25343
                     /locus_tag="ACHGNW_001073"
     CDS             24669..25343
                     /locus_tag="ACHGNW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6925403.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25348..26688
                     /locus_tag="ACHGNW_001074"
     CDS             25348..26688
                     /locus_tag="ACHGNW_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6925404.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26837..27823)
                     /locus_tag="ACHGNW_001075"
     CDS             complement(26837..27823)
                     /locus_tag="ACHGNW_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN6925405.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8133 a         5269 c         5057 g         9444 t
ORIGIN      
        1 ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa
       61 ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac
      121 cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc
      181 gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta
      241 atcaacagat taaatttcat atgttcatat gtacggatac attttcggac cgattaattt
      301 taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat
      361 ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt
      421 gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt
      481 gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt
      541 aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg
      601 ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt
      661 tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc
      721 taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc
      781 gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc
      841 gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt
      901 atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc
      961 gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga
     1021 tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa
     1081 acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt
     1141 acattcttca tagccacctg accatacagc cacattttca tcgccccaag ccataccatc
     1201 taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc
     1261 attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc
     1321 gacgattact actttacgac ttgttttttt catgaataaa cagctccttt tcataactat
     1381 ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa
     1441 tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga
     1501 caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat
     1561 ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct
     1621 tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac
     1681 atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat
     1741 tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt
     1801 ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa
     1861 gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat
     1921 gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg
     1981 aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat
     2041 ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca
     2101 aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt
     2161 tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat
     2221 tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt
     2281 gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt
     2341 ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac atataattga
     2401 tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat
     2461 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
     2521 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
     2581 cttcgttacg caaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta
     2641 agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg
     2701 gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga
     2761 ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat
     2821 gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg
     2881 tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc
     2941 tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag
     3001 ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc
     3061 gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc
     3121 tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt
     3181 ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg
     3241 tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt
     3301 tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc
     3361 tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa tcaaaacgaa
     3421 tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt
     3481 atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc
     3541 gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct
     3601 tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt
     3661 tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg
     3721 aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa
     3781 gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat
     3841 tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt
     3901 tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca
     3961 caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac
     4021 aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct
     4081 ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga
     4141 acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt
     4201 taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg
     4261 gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg
     4321 taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga
     4381 gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc
     4441 agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca
     4501 aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt
     4561 tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat
     4621 cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa
     4681 gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat
     4741 accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc
     4801 gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga
     4861 agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg
     4921 gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga
     4981 ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat
     5041 gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat
     5101 gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata
     5161 cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt
     5221 tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact
     5281 gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa
     5341 cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt
     5401 tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag
     5461 aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca
     5521 acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc
     5581 atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta
     5641 ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga
     5701 agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg
     5761 aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat
     5821 cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc
     5881 atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac
     5941 aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa
     6001 aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc
     6061 ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc
     6121 caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt
     6181 tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact
     6241 gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa
     6301 tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat
     6361 aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta
     6421 ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc
     6481 ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca
     6541 aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga
     6601 acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga
     6661 acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat
     6721 gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca
     6781 acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg
     6841 atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta
     6901 gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt
     6961 ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa
     7021 agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac
     7081 aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc
     7141 attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc
     7201 acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc
     7261 gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc
     7321 gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat
     7381 ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg
     7441 agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac
     7501 aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa
     7561 gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag
     7621 cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc
     7681 agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc
     7741 tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa
     7801 tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta
     7861 atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta
     7921 gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga
     7981 tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca
     8041 gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt
     8101 gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat
     8161 aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt
     8221 gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca
     8281 taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat
     8341 agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca
     8401 attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga
     8461 tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct
     8521 tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct
     8581 cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa
     8641 gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta
     8701 tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga
     8761 aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg
     8821 cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat
     8881 taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat
     8941 tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga
     9001 tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa
     9061 cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc
     9121 caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag
     9181 catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat
     9241 aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat
     9301 ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga
     9361 gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc
     9421 ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac
     9481 aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag
     9541 aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca
     9601 tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat
     9661 ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat
     9721 ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga
     9781 tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt
     9841 agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag
     9901 gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc
     9961 gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg
    10021 tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca
    10081 tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc
    10141 tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt
    10201 gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc
    10261 acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca
    10321 taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag
    10381 tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc
    10441 ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt
    10501 gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta
    10561 atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt
    10621 caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa
    10681 cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc
    10741 gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt
    10801 catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag
    10861 ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac
    10921 tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc
    10981 tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt
    11041 tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta
    11101 acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga
    11161 cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc
    11221 atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc
    11281 ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc
    11341 agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa
    11401 atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc
    11461 ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct
    11521 attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt
    11581 caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag
    11641 aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca
    11701 cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga
    11761 cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca
    11821 aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc
    11881 aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata
    11941 gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac
    12001 aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc
    12061 gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa
    12121 tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg
    12181 cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc
    12241 attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg
    12301 gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg
    12361 aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa
    12421 atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa
    12481 acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga
    12541 tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa
    12601 acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag
    12661 atatattata gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa
    12721 aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa
    12781 tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac
    12841 ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag
    12901 tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt
    12961 cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc
    13021 acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct
    13081 ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga
    13141 ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat
    13201 ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc
    13261 aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg
    13321 ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac
    13381 cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct
    13441 aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc
    13501 tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata
    13561 aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact
    13621 aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc
    13681 ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt
    13741 tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga
    13801 tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca
    13861 gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa
    13921 aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg
    13981 ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg
    14041 tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat
    14101 cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa
    14161 atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac
    14221 cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt
    14281 gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat aaggcataag
    14341 catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt
    14401 tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga
    14461 cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg
    14521 ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat
    14581 ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag
    14641 cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt
    14701 tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga
    14761 aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga
    14821 tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta
    14881 gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg
    14941 tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat
    15001 ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca
    15061 ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag
    15121 gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag
    15181 gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg
    15241 cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt
    15301 gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca
    15361 ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc
    15421 cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt
    15481 ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa
    15541 gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg
    15601 aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt
    15661 atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc
    15721 agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa
    15781 agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta
    15841 tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc
    15901 agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata
    15961 tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat
    16021 ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt
    16081 tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt
    16141 gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg
    16201 taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat
    16261 atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt
    16321 tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt
    16381 aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac
    16441 taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata
    16501 cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc
    16561 gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt
    16621 tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga
    16681 atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa
    16741 agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa
    16801 tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc
    16861 tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag
    16921 ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc
    16981 cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc
    17041 tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca
    17101 tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt aactggctct
    17161 tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt
    17221 aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt
    17281 tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg
    17341 tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt
    17401 gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca
    17461 taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta
    17521 ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt
    17581 ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt
    17641 atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa
    17701 ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg
    17761 gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc
    17821 ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt
    17881 tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct
    17941 gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt
    18001 ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga
    18061 gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga
    18121 ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat
    18181 ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga
    18241 atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta
    18301 acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga
    18361 agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca
    18421 atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc
    18481 tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa
    18541 tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta
    18601 ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg
    18661 attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct
    18721 aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca
    18781 tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc
    18841 ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc
    18901 atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg
    18961 tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta
    19021 gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag
    19081 gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg
    19141 gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt
    19201 gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca
    19261 ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct
    19321 gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg
    19381 atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc
    19441 gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta
    19501 cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat
    19561 tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat accatcccat
    19621 tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc
    19681 caactggctt taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga
    19741 ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa
    19801 ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct
    19861 gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc
    19921 ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt
    19981 ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca
    20041 tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct
    20101 atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac
    20161 tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct
    20221 gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact
    20281 ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac
    20341 gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta ttttaatatg
    20401 ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc
    20461 atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga
    20521 gaaaaacata tgatcaggtt gttggaagga aactatagaa agatggaaaa aatcattcga
    20581 attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt
    20641 tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta
    20701 acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat
    20761 ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt
    20821 attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct
    20881 tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg
    20941 atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt
    21001 gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa
    21061 aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa
    21121 agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc
    21181 caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat
    21241 aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata
    21301 aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat
    21361 gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc
    21421 aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt
    21481 attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg
    21541 caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact
    21601 ctactgaatc agaatctgat ttattattta tctagctata tcttttggat gcttatcggt
    21661 aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt
    21721 cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt
    21781 cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat
    21841 gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat
    21901 gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc
    21961 aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt
    22021 ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa
    22081 ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat
    22141 ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct
    22201 taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt
    22261 tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg
    22321 atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt
    22381 ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata
    22441 caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat
    22501 cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag
    22561 taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg
    22621 catccacatc tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga
    22681 ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat
    22741 cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat
    22801 gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg
    22861 ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc
    22921 tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta
    22981 aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa
    23041 ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa
    23101 atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt
    23161 gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga
    23221 aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat
    23281 aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa
    23341 cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc
    23401 tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat
    23461 cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat
    23521 cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa
    23581 ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac
    23641 tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc
    23701 aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc
    23761 aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc
    23821 tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga
    23881 agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac
    23941 gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta
    24001 actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat
    24061 gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc
    24121 ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct
    24181 gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa
    24241 ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca
    24301 atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa
    24361 tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata
    24421 gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga
    24481 caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt
    24541 ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa
    24601 aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag
    24661 gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa
    24721 aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt
    24781 tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga
    24841 atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt
    24901 tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt
    24961 atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac
    25021 gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa
    25081 aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat
    25141 tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc
    25201 ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact
    25261 tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg
    25321 gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt
    25381 cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt
    25441 actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata
    25501 aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt
    25561 ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca
    25621 tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat
    25681 aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat
    25741 ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc
    25801 cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta
    25861 gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt
    25921 atgattgttt tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg
    25981 cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa
    26041 ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat
    26101 catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc
    26161 actggattga cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt
    26221 tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag
    26281 atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca
    26341 attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat
    26401 acgaatccag aagataagat cattttggat tcatctgtta accataaaga atggctgatt
    26461 cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga
    26521 ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca
    26581 attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca
    26641 caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta
    26701 aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc
    26761 cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa
    26821 cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg
    26881 tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt
    26941 cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct
    27001 cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag
    27061 attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta
    27121 ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat
    27181 gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt
    27241 tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca
    27301 tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc
    27361 cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg
    27421 ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc
    27481 cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat
    27541 cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt
    27601 tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta
    27661 taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga
    27721 tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc
    27781 tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca
    27841 aatagattta tgattggtat attgttactt ataaatttat tctataggag acgattgttt
    27901 tct
//
LOCUS       DAZBTH010000025        27724 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000025 DAZBTH010000000
VERSION     DAZBTH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.025"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..2060
                     /locus_tag="ACHGNW_001076"
     CDS             294..2060
                     /locus_tag="ACHGNW_001076"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN6925406.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2162..3421
                     /locus_tag="ACHGNW_001077"
     CDS             2162..3421
                     /locus_tag="ACHGNW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6925407.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3568..4869
                     /locus_tag="ACHGNW_001078"
     CDS             3568..4869
                     /locus_tag="ACHGNW_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925408.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4872..5723
                     /locus_tag="ACHGNW_001079"
     CDS             4872..5723
                     /locus_tag="ACHGNW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN6925409.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5736..6551
                     /locus_tag="ACHGNW_001080"
     CDS             5736..6551
                     /locus_tag="ACHGNW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN6925410.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6702..7868
                     /locus_tag="ACHGNW_001081"
     CDS             6702..7868
                     /locus_tag="ACHGNW_001081"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6925411.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8098..8745
                     /locus_tag="ACHGNW_001082"
     CDS             8098..8745
                     /locus_tag="ACHGNW_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6925412.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8829..9773
                     /locus_tag="ACHGNW_001083"
     CDS             8829..9773
                     /locus_tag="ACHGNW_001083"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN6925413.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9908..11359
                     /locus_tag="ACHGNW_001084"
     CDS             9908..11359
                     /locus_tag="ACHGNW_001084"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN6925414.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11380..12216
                     /locus_tag="ACHGNW_001085"
     CDS             11380..12216
                     /locus_tag="ACHGNW_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6925415.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12216..12875
                     /locus_tag="ACHGNW_001086"
     CDS             12216..12875
                     /locus_tag="ACHGNW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6925416.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            13001..13690
                     /locus_tag="ACHGNW_001087"
     CDS             13001..13690
                     /locus_tag="ACHGNW_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925417.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13696..14652
                     /locus_tag="ACHGNW_001088"
     CDS             13696..14652
                     /locus_tag="ACHGNW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN6925418.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14696..15208
                     /locus_tag="ACHGNW_001089"
     CDS             14696..15208
                     /locus_tag="ACHGNW_001089"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925419.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15464..16012)
                     /locus_tag="ACHGNW_001090"
     CDS             complement(15464..16012)
                     /locus_tag="ACHGNW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6925420.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16101..17282)
                     /locus_tag="ACHGNW_001091"
     CDS             complement(16101..17282)
                     /locus_tag="ACHGNW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN6925421.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17518..19026)
                     /locus_tag="ACHGNW_001092"
     CDS             complement(17518..19026)
                     /locus_tag="ACHGNW_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN6925422.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19185..19376
                     /locus_tag="ACHGNW_001093"
     CDS             19185..19376
                     /locus_tag="ACHGNW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925423.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19579..20496
                     /locus_tag="ACHGNW_001094"
     CDS             19579..20496
                     /locus_tag="ACHGNW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN6925424.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20489..21562
                     /locus_tag="ACHGNW_001095"
     CDS             20489..21562
                     /locus_tag="ACHGNW_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925425.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21710..22228
                     /locus_tag="ACHGNW_001096"
     CDS             21710..22228
                     /locus_tag="ACHGNW_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925426.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22209..22601
                     /locus_tag="ACHGNW_001097"
     CDS             22209..22601
                     /locus_tag="ACHGNW_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN6925427.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22677..23612
                     /locus_tag="ACHGNW_001098"
     CDS             22677..23612
                     /locus_tag="ACHGNW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6925428.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23745..24524
                     /locus_tag="ACHGNW_001099"
     CDS             23745..24524
                     /locus_tag="ACHGNW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6925429.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24517..24834
                     /locus_tag="ACHGNW_001100"
     CDS             24517..24834
                     /locus_tag="ACHGNW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6925430.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25078..26688
                     /locus_tag="ACHGNW_001101"
     CDS             25078..26688
                     /locus_tag="ACHGNW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6925431.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26845..27693
                     /locus_tag="ACHGNW_001102"
     CDS             26845..27693
                     /locus_tag="ACHGNW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6925432.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8984 a         4902 c         5553 g         8285 t
ORIGIN      
        1 tctaaattat tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa
       61 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
      121 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
      181 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
      241 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
      301 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
      361 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
      421 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
      481 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
      541 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
      601 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
      661 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
      721 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
      781 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
      841 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
      901 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
      961 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
     1021 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
     1081 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
     1141 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
     1201 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
     1261 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
     1321 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
     1381 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
     1441 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
     1501 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
     1561 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
     1621 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
     1681 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
     1741 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
     1801 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
     1861 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
     1921 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
     1981 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
     2041 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
     2101 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
     2161 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
     2221 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
     2281 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
     2341 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
     2401 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
     2461 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
     2521 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
     2581 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
     2641 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
     2701 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
     2761 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
     2821 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
     2881 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
     2941 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
     3001 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
     3061 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
     3121 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
     3181 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
     3241 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
     3301 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
     3361 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
     3421 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
     3481 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
     3541 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
     3601 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
     3661 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
     3721 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
     3781 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
     3841 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
     3901 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
     3961 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
     4021 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
     4081 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
     4141 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
     4201 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
     4261 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
     4321 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
     4381 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
     4441 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
     4501 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
     4561 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
     4621 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
     4681 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
     4741 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
     4801 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
     4861 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
     4921 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
     4981 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
     5041 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
     5101 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
     5161 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
     5221 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
     5281 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
     5341 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
     5401 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
     5461 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
     5521 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
     5581 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
     5641 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
     5701 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
     5761 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
     5821 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
     5881 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
     5941 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
     6001 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
     6061 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
     6121 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
     6181 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
     6241 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
     6301 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
     6361 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
     6421 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
     6481 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
     6541 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
     6601 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
     6661 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
     6721 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
     6781 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
     6841 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
     6901 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
     6961 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
     7021 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
     7081 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
     7141 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
     7201 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
     7261 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
     7321 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
     7381 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
     7441 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
     7501 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
     7561 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
     7621 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
     7681 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
     7741 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
     7801 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
     7861 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
     7921 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct
     7981 ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat
     8041 tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg
     8101 aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca
     8161 aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag
     8221 gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt
     8281 gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa
     8341 gataaatacc cagatactgc aattactagt ttggaattag atagtgattg gggatcttac
     8401 ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa
     8461 aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa
     8521 aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa
     8581 atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta
     8641 ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc
     8701 aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa
     8761 acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag
     8821 cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga
     8881 atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag
     8941 caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac
     9001 gctattttga tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt
     9061 cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt
     9121 tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag
     9181 aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa
     9241 aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca
     9301 tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc
     9361 agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg
     9421 gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt
     9481 tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt
     9541 atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag
     9601 atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata
     9661 aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa
     9721 aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag
     9781 acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg
     9841 tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga
     9901 ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt
     9961 tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc
    10021 gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga
    10081 aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg
    10141 atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa
    10201 ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga
    10261 gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa
    10321 ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat
    10381 cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg
    10441 cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag
    10501 aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc
    10561 tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga
    10621 gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat
    10681 ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg
    10741 aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac
    10801 gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg
    10861 catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata
    10921 tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc
    10981 aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt
    11041 taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca
    11101 catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag
    11161 cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag caatcagctt
    11221 gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg
    11281 aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt
    11341 accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt
    11401 gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag
    11461 gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc
    11521 acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat
    11581 ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg
    11641 gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt
    11701 atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac
    11761 agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg
    11821 aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct
    11881 aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt
    11941 cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca
    12001 gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta
    12061 ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa
    12121 gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa
    12181 ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttccccta attgattttt
    12241 ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg
    12301 gcttcgtgtc gtttctttta ggaaatttgg tagggttgct tttgacagtt ttaggtcttt
    12361 tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc
    12421 ccgcactagt cctgctgttt ctgttatatt tcgggttacc ttatcaatta agtgccttaa
    12481 cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag
    12541 gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt
    12601 tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac
    12661 tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc
    12721 cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct
    12781 atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt
    12841 atttagaaaa acgttctatc catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc
    12901 tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca
    12961 tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat
    13021 cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga
    13081 agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga
    13141 tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac
    13201 agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga
    13261 agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt
    13321 tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga
    13381 gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt
    13441 aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaaaagatc aattgtacaa
    13501 agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg
    13561 gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct
    13621 tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc
    13681 attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca
    13741 ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca
    13801 gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca
    13861 atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac
    13921 agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat
    13981 ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca
    14041 aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat
    14101 actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa
    14161 acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat
    14221 ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt
    14281 tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct
    14341 tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa
    14401 gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat
    14461 caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc
    14521 cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac
    14581 ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa
    14641 gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta
    14701 tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc
    14761 ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga
    14821 aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat
    14881 tgctggaaca gctgcattag tctcaggcgt ggatcccgtt tacacaaaaa tagatggtag
    14941 ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac
    15001 tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa
    15061 aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat
    15121 ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag
    15181 aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat
    15241 taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc
    15301 ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt
    15361 attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt
    15421 gtcacgatct ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt
    15481 tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg
    15541 ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg
    15601 aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga
    15661 tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat
    15721 ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac
    15781 tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca
    15841 tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc
    15901 ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga
    15961 gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt
    16021 ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag
    16081 taactttgct tttttgtatt ttataccagc aagccagtca caatagcact taaaaagctg
    16141 actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct
    16201 tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa
    16261 acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct
    16321 aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca
    16381 tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct
    16441 aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc
    16501 aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga
    16561 atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca
    16621 tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata accgccaaat
    16681 aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg
    16741 acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca
    16801 gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca
    16861 gaagctactc cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga
    16921 ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca
    16981 gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct
    17041 acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt
    17101 tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga
    17161 acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg
    17221 ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc
    17281 aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta
    17341 atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa
    17401 ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa
    17461 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
    17521 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
    17581 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
    17641 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
    17701 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
    17761 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
    17821 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
    17881 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
    17941 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
    18001 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
    18061 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
    18121 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
    18181 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
    18241 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
    18301 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
    18361 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
    18421 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagc aagtagcgag
    18481 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
    18541 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
    18601 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
    18661 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
    18721 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
    18781 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
    18841 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
    18901 atgcttgtcg tcaactaact tcccaccaaa ttctaaatat aatttgtcat agtggttcac
    18961 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
    19021 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
    19081 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
    19141 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
    19201 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
    19261 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
    19321 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
    19381 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
    19441 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
    19501 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
    19561 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
    19621 ctagaggaga cgaacgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
    19681 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
    19741 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
    19801 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
    19861 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
    19921 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
    19981 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
    20041 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
    20101 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
    20161 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
    20221 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
    20281 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
    20341 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
    20401 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
    20461 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
    20521 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
    20581 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacatgaaaa
    20641 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
    20701 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
    20761 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
    20821 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
    20881 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
    20941 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
    21001 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
    21061 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
    21121 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
    21181 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
    21241 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
    21301 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
    21361 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
    21421 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
    21481 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
    21541 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
    21601 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
    21661 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
    21721 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
    21781 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
    21841 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
    21901 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
    21961 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
    22021 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
    22081 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
    22141 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
    22201 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
    22261 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
    22321 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
    22381 tggtcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
    22441 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
    22501 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
    22561 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
    22621 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
    22681 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
    22741 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
    22801 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
    22861 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
    22921 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
    22981 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
    23041 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
    23101 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
    23161 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
    23221 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
    23281 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
    23341 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
    23401 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
    23461 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
    23521 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
    23581 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
    23641 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
    23701 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
    23761 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
    23821 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
    23881 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
    23941 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
    24001 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
    24061 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
    24121 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
    24181 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
    24241 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
    24301 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
    24361 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
    24421 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
    24481 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
    24541 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
    24601 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
    24661 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
    24721 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
    24781 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
    24841 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
    24901 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
    24961 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
    25021 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
    25081 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
    25141 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
    25201 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
    25261 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
    25321 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
    25381 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
    25441 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
    25501 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
    25561 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
    25621 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
    25681 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
    25741 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
    25801 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
    25861 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
    25921 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
    25981 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
    26041 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
    26101 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
    26161 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
    26221 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
    26281 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
    26341 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
    26401 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
    26461 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
    26521 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
    26581 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
    26641 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
    26701 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
    26761 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
    26821 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
    26881 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
    26941 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
    27001 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
    27061 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
    27121 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
    27181 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
    27241 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
    27301 gataatacct atgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
    27361 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
    27421 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    27481 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    27541 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    27601 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    27661 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    27721 aata
//
LOCUS       DAZBTH010000026        27547 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000026 DAZBTH010000000
VERSION     DAZBTH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.026"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHGNW_001103"
     CDS             complement(407..766)
                     /locus_tag="ACHGNW_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN6925433.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHGNW_001104"
     CDS             complement(796..1137)
                     /locus_tag="ACHGNW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925434.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHGNW_001105"
     CDS             complement(1134..1808)
                     /locus_tag="ACHGNW_001105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6925435.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHGNW_001106"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHGNW_001106"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN6925436.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHGNW_001107"
     CDS             complement(4322..5512)
                     /locus_tag="ACHGNW_001107"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6925437.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHGNW_001108"
     CDS             complement(5533..6060)
                     /locus_tag="ACHGNW_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN6925438.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHGNW_001109"
     CDS             complement(6107..8896)
                     /locus_tag="ACHGNW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN6925439.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHGNW_001110"
     CDS             complement(9043..9243)
                     /locus_tag="ACHGNW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6925440.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHGNW_001111"
     CDS             complement(9696..10016)
                     /locus_tag="ACHGNW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925441.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACHGNW_001112"
     CDS             complement(10519..11637)
                     /locus_tag="ACHGNW_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925442.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHGNW_001113"
     CDS             11870..11992
                     /locus_tag="ACHGNW_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925443.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHGNW_001114"
     CDS             12108..13415
                     /locus_tag="ACHGNW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN6925444.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHGNW_001115"
     CDS             13536..14735
                     /locus_tag="ACHGNW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6925445.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHGNW_001116"
     CDS             complement(14762..16030)
                     /locus_tag="ACHGNW_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN6925446.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHGNW_001117"
     CDS             complement(16200..16841)
                     /locus_tag="ACHGNW_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN6925447.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHGNW_001118"
     CDS             complement(17250..17753)
                     /locus_tag="ACHGNW_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6925448.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHGNW_001119"
     CDS             complement(17876..18640)
                     /locus_tag="ACHGNW_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN6925449.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHGNW_001120"
     CDS             18748..19023
                     /locus_tag="ACHGNW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN6925450.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACHGNW_001121"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACHGNW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN6925451.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACHGNW_001122"
     CDS             complement(20579..20980)
                     /locus_tag="ACHGNW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6925452.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACHGNW_001123"
     CDS             complement(20994..21803)
                     /locus_tag="ACHGNW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6925453.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACHGNW_001124"
     CDS             complement(21790..22680)
                     /locus_tag="ACHGNW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6925454.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACHGNW_001125"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACHGNW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACHGNW_001126"
     CDS             complement(23348..24289)
                     /locus_tag="ACHGNW_001126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN6925455.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHGNW_001127"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHGNW_001127"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN6925456.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACHGNW_001128"
     CDS             complement(25274..26470)
                     /locus_tag="ACHGNW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6925457.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACHGNW_001129"
     CDS             complement(26488..27216)
                     /locus_tag="ACHGNW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6925458.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         7998 a         5889 c         4779 g         8881 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattatt
//
LOCUS       DAZBTH010000027        26312 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000027 DAZBTH010000000
VERSION     DAZBTH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.027"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..554)
                     /locus_tag="ACHGNW_001130"
     CDS             complement(186..554)
                     /locus_tag="ACHGNW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN6925459.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            704..1186
                     /locus_tag="ACHGNW_001131"
     CDS             704..1186
                     /locus_tag="ACHGNW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN6925460.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1223..2233
                     /locus_tag="ACHGNW_001132"
     CDS             1223..2233
                     /locus_tag="ACHGNW_001132"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN6925461.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2348..3061)
                     /locus_tag="ACHGNW_001133"
     CDS             complement(2348..3061)
                     /locus_tag="ACHGNW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925462.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3234..3841)
                     /locus_tag="ACHGNW_001134"
                     /pseudo
     CDS             complement(3234..3841)
                     /locus_tag="ACHGNW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4325..4552
                     /locus_tag="ACHGNW_001135"
     CDS             4325..4552
                     /locus_tag="ACHGNW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN6925463.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4568..5734
                     /gene="hemW"
                     /locus_tag="ACHGNW_001136"
     CDS             4568..5734
                     /gene="hemW"
                     /locus_tag="ACHGNW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN6925464.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5968..7011
                     /gene="hrcA"
                     /locus_tag="ACHGNW_001137"
     CDS             5968..7011
                     /gene="hrcA"
                     /locus_tag="ACHGNW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN6925465.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7105..7668
                     /gene="grpE"
                     /locus_tag="ACHGNW_001138"
     CDS             7105..7668
                     /gene="grpE"
                     /locus_tag="ACHGNW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN6925466.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7719..9548
                     /gene="dnaK"
                     /locus_tag="ACHGNW_001139"
     CDS             7719..9548
                     /gene="dnaK"
                     /locus_tag="ACHGNW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN6925467.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9699..10865
                     /gene="dnaJ"
                     /locus_tag="ACHGNW_001140"
     CDS             9699..10865
                     /gene="dnaJ"
                     /locus_tag="ACHGNW_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN6925468.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10947..11576)
                     /locus_tag="ACHGNW_001141"
     CDS             complement(10947..11576)
                     /locus_tag="ACHGNW_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925469.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11588..11761
                     /locus_tag="ACHGNW_001142"
     CDS             11588..11761
                     /locus_tag="ACHGNW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925470.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11806..12543)
                     /locus_tag="ACHGNW_001143"
     CDS             complement(11806..12543)
                     /locus_tag="ACHGNW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6925471.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12545..14047)
                     /locus_tag="ACHGNW_001144"
     CDS             complement(12545..14047)
                     /locus_tag="ACHGNW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN6925472.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14306..16300
                     /gene="uvrB"
                     /locus_tag="ACHGNW_001145"
     CDS             14306..16300
                     /gene="uvrB"
                     /locus_tag="ACHGNW_001145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN6925473.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16310..19126
                     /gene="uvrA"
                     /locus_tag="ACHGNW_001146"
     CDS             16310..19126
                     /gene="uvrA"
                     /locus_tag="ACHGNW_001146"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN6925474.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19389..20654
                     /locus_tag="ACHGNW_001147"
     CDS             19389..20654
                     /locus_tag="ACHGNW_001147"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN6925475.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20658..21389
                     /locus_tag="ACHGNW_001148"
     CDS             20658..21389
                     /locus_tag="ACHGNW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN6925476.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21524..22408
                     /gene="rapZ"
                     /locus_tag="ACHGNW_001149"
     CDS             21524..22408
                     /gene="rapZ"
                     /locus_tag="ACHGNW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN6925477.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22405..23403
                     /gene="yvcK"
                     /locus_tag="ACHGNW_001150"
     CDS             22405..23403
                     /gene="yvcK"
                     /locus_tag="ACHGNW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN6925478.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23437..24372
                     /gene="whiA"
                     /locus_tag="ACHGNW_001151"
     CDS             23437..24372
                     /gene="whiA"
                     /locus_tag="ACHGNW_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN6925479.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24915..25565
                     /locus_tag="ACHGNW_001152"
     CDS             24915..25565
                     /locus_tag="ACHGNW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925480.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25752..>26312
                     /locus_tag="ACHGNW_001153"
     CDS             25752..>26312
                     /locus_tag="ACHGNW_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFN6925481.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8688 a         4443 c         5594 g         7587 t
ORIGIN      
        1 gtagaaaatt caaagccatt caatcagtga tttttttcag cacaagaaag actccttata
       61 aaaatagcag atactttgtc aattaagtgt ttaaccaggg atgataaaaa ggataggatt
      121 gaacaaaacc tgtataaggt gatgaatagt ttcctcgcca gtaaaaggtt tattcgtata
      181 aagctttatc gccaaaattt atcatgacct tttttatctt caatattaaa tgaaatcaat
      241 tgttcaatca attcccagtt gattacactt ttccaaggaa ctttgatgac gttatccgta
      301 tatacgtagc ctgcttcttg gatatttttt tcaagggcac gaatcgctgc tttttccggt
      361 gcgatagaaa aatgattttt agcaaaacta aatgcaataa taaaggtacc atggtcagta
      421 aacataggtt ggttccattt tacagttgtg tctagctggg gatacgtttc tgttatctta
      481 gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt tgatttgatt gatgatcggc
      541 tgaaattttt ccattctaaa acgcctccat taagtggttt gatttatttt accagaaaaa
      601 caatagttat gtataaaaaa cgaacaaaac acaagtaatg ttgtaaccgc tatatttata
      661 tgataagatg tctcaaataa taaaatagaa aagagacata aaaatgattg acttggcaaa
      721 gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca tttatacaaa
      781 tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga ccaaaagctt
      841 attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta cgaaacaaac
      901 ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt catcagaacg
      961 catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt atgaaaaaca
     1021 gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt ttgcaccagg
     1081 tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca tccagctact
     1141 tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa gtaaaatagt
     1201 gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa atgcaacgat
     1261 tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac tcgaatcagc
     1321 tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc tttggacgca
     1381 agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc aaatgatgca
     1441 agatgaaaat atccaaatga tctttccgac ttggggagga gaattattga ttgagattct
     1501 cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt cagatattag
     1561 tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg gaactaacct
     1621 tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa gtgtactgaa
     1681 aacaagagaa ggagaatcga ttctacagca atcttctgaa aagtatcaaa aagtgtggcc
     1741 ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt ggcaatcagt
     1801 cagcggagat gcagtaagga tgaagggacg attattaggt ggttgtattg atacgatcag
     1861 gcatttaatc ggtactcctt atggagatgt ccaatctttt aaaagaaagt ataatgatgg
     1921 aaagccgatc ttgtggtttc tagaaaactg tgagctatcg tcagttgatt taagaagatc
     1981 acttgttcag atgaaactgg caggctggtt tgatggcagc tcggggatca tgttcggtag
     2041 aagtgcggca gaggatttgt tagattataa aatgaaggat gtatacgaag atctgtcaaa
     2101 ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc cacaactgac
     2161 attgattaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga ctgttttgca
     2221 aacgtttatt taaaaaattt tttcctgcct tggagtctat caaagaccaa taaatctaaa
     2281 tagaaaagaa agtgaaagaa gctgacaaaa tgctgtcagc ttctttttgt ataacatatt
     2341 agaaagtcta attatctata actgtcacat cgattaactg tttcacgtca ttgggatttc
     2401 ctgtaggaat agctactaca gatgcattat caatgatttc ttgagttgtt tctctaatga
     2461 acgctggata agatgtttga tctgcaggca tttgccaatg tgaaggaact ggataactag
     2521 tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg tatttcccag
     2581 tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg gcgataggtg
     2641 ttcctgcagg ataatgaata acatgtaatt cgaaatcacc cgatgtatct ctaaaagtat
     2701 tacctctaat agctagccaa acacgtgcgt actcaatttg tagatcagaa tatcctgcta
     2761 acggatctat tgactgagaa gactgtgagg aggttgtgtc tattgttgaa gaagaaatat
     2821 ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact ttactgcttg
     2881 ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt tcagctgaac
     2941 tggtttgtct gattgaactt ttgtctgtgg tcgcagatgg atcactattt tctttactag
     3001 gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct aactttttca
     3061 tataaaatac ctcattattt ttttaatacc agtatagcat aatcctgaat ttttgaatcc
     3121 gtaaaacctt gtctatgaat ataaatagag tgtatcttct ttgataaatg tcagaaaaag
     3181 tgtgacaaat aatcatcagt aaaattactt gtcacactat tgataaggag tatttaattg
     3241 aagagaatga ttaggagaaa ggatctccgt actttttata gtaatcatag aatccaagtt
     3301 tgtatagacg aatcgttttt tctcttattt cttctgtttc aaaatggact gcttgaagaa
     3361 tcaagtctgt gaattctata ggagctgttc cattcgcagt aatcaaatta cgatctttca
     3421 cagcttgttg ttctataaaa tcttctttat tctgataatt ttccgattct aaccaaggaa
     3481 attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc gctctgagct aaataatcaa
     3541 ctgccccaca aatagcagct actggtacgt ctgtttttaa caatttatcg attatttgat
     3601 ataaatgatg attttttatg tcccatgaat tgccaccgat cataataaaa aggtcaactt
     3661 tttcaggtat ttgttcgatt aagtaatcga tttttgtttt taatcctcca atggaagtga
     3721 cctctgattg gatagaagca gttttgtttt gccatttctc attttggttt agtgtgctag
     3781 taatataagc accttcccaa tcggcatatt cctctaatag aaacgtaatc gcagttttca
     3841 ttttcaagct ctcctttact atctataaca aggacaacag ttttaaccta cagacattca
     3901 ttaaaattgg gtagcgacag cgaaaacaac aattcttaag ttttagacag agtatacaga
     3961 tagtcaactt aattaaatac gaactaaata aaaatgacag gtaatcattt ttgtttacca
     4021 gcattataac attttgcata catggatgac aaaagaacca attcccactt gcttgagtac
     4081 aatattaaaa gaaactggaa ggcaaaaagt aattatagaa acgaaaaata aatcatatcg
     4141 ggcactcaga catgaaaaca accctagaga tatataacta ggtaaccagt ttaatgaaag
     4201 aaaaaatcat ccaagaagtc gattcttgga ttttttagtg gatttgttcg ccaaatacgt
     4261 ttagtatgcg tatatgagag gttgtaagca aagcgttgtg gtctgagcac ttaaggagga
     4321 atctgtgaaa atagcttttc atatttttgc agattttgac ttaatgccga aggtcagctt
     4381 tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt agctccgatg acaataagca
     4441 agcattttta gagctaggtt tccttgattc taaatatgcg gcttatttca atgagttggt
     4501 gcttgaacgc gattttaaga aggtcgaaaa caggcacgac ttttcgacgt aaggagaaag
     4561 aatttcaatg atagaaacaa taaatcccgc agaaaacaag gtttctgcgt acattcatat
     4621 cccattttgc gagcatatct gctattactg tgattttaat aaagtttttt tagaagggca
     4681 gccagtagat gagtatgtgg aaatgctgct gaatgaaatg aaaataatgc tagaaagtca
     4741 tccgacagcg aatttagaaa cactttacgt agggggcggg acaccaactt ctttatctgc
     4801 taaacaactt gatcgactgt tatcaggtgc tagagagatc ctgccttttc aaaatggtaa
     4861 tgagtttact gtagaagcga atccaggtga tttgacccgt gaaaaacttc aagtaatgaa
     4921 aaactatggc gtcaatcgtt tatccatggg agtgcagaca tttgataatc gcttattgaa
     4981 aaaaattgga cgtaaacaca ccgcagaaga tgtgtatcaa actatgcaat ttttagaagc
     5041 agaaaatttt accaatgtaa gcatcgattt gatttatgca ttaccgggac aaacactaga
     5101 aggatatctg gatactttgg aaaaagcttt ggctttggat ctgcctcact attctttgta
     5161 ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag gaagactcga
     5221 actgcctgat caggaaacgg aaaccagaat gtttgaagag acgattgaag caatggaaaa
     5281 gcatggacgt caccaatatg aaataagtaa ctttgggctc tccggacatg aatctcaaca
     5341 taacctcatg tactggaaca atgaccacta ttttgggttt ggagctggtg ctagcggtta
     5401 tcttggagat attcgttacc gcaacaaagg accaatccaa cactatttac gcccattacg
     5461 agcaaatgaa cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc aaatagaaga
     5521 agaaatgttc ttaggactaa gaaaaaaaac agggattact aaaaaacatt tctatgatcg
     5581 ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta agggaattag agcaagaagg
     5641 gttacttgtt aatgaagaag accgtgttta tttgacacca agagggacct ttttggggaa
     5701 caatgtgttt gagcgctttt tgtttgaaaa atagtttaaa gaatatttgt tttttatctg
     5761 aatttaaata aaaaaagttc aaaaatacaa taaaggacta ttgaaacgtt gatataactg
     5821 gatttacatc aattatttag aattagcact cttgtgttta gagtgctaaa aaatggagtc
     5881 tttctcttga caggagaagg gcttcttgct atatttataa gtggattagc acttagaata
     5941 tatgagtgct aatgagaggt gaggaagatg ttaacaaagc gacaaaacga cattttacgt
     6001 ctgatcatcc aacactatac gagtagtggc gtgcccgttg gttccaaaac attgatgtct
     6061 gagggagtca aggccagctc agcaacgatt cgcaatgata tgaaagcatt agaagatgaa
     6121 ggattgctgt tgaagactca ctcttcttct gggcggattc catctgcttt aggctatcgt
     6181 tattacgtgg atcatttgtt aaaacctgct cgcgtagcag aagatgattt gctgctgatt
     6241 cgtcagtctc taggcagaga atttcatgaa atcaatgaga ttattaagca atctgctgag
     6301 atattatctg agctgacaag ctatacgact ttttcattgg gacctgaaat aaaagaccgc
     6361 cgactgactg gctttcgtat cgtacccttg aataatcgac agattattgc tatcgtcgta
     6421 acagatatgg ggaatgtaga aagccaagtg ttcactcttc ccgaatatat gggaagtcag
     6481 gaactagaaa aaatggtaca gattgtaaac gaccgtctag tcggagatcc tctgatgacg
     6541 gtgtaccata aacttcgtac ggagattccg atgattcttc ataaatattt ccaaacgaca
     6601 gaagggatgt ctcagttatt tgacaccgtt cttggtcatg tttttgaaga taaggtttat
     6661 gtcagtggac aaatgaatct cttggattat gagccccgtc aagatcttga tcagttcaaa
     6721 tcgatgtttt cttttatgaa agactcagac ggacttacac aaatgatcac tccgatggat
     6781 agcaatattc atatcaagat tggtgctgaa ttaggcaatg atttattgca gaatatgagt
     6841 atgattcaag caagttacga gatagatggt catggaagag gtacaatagc cttgttaggg
     6901 cctactagta tgccttattc tcggatgttc ggtctagtcg atgtttatcg aagagaacta
     6961 gcagctaaac ttgctgatta ctaccgttcg cttgacttgt caaactcgta atgtgaataa
     7021 cgaagctatt tgcagctgat cgttctctct ttcttagtaa gagcaatcaa acagctgact
     7081 gaataatgaa aggaagactg tatcgtgaaa gaaaatcaag aagaactaga taaagaaatg
     7141 acagatgctc agccagaacc ggaaatcgat gtcgaagctg cagaagattc gggtatttca
     7201 gaagctgaag cggaagaatt cgaaacagcg aaattaaaag ctgagttaga agaaatggaa
     7261 gataaatact tgcgggctcg tgctgaaatc gcaaatatgg caaatcgagg aaaaaatgag
     7321 cgtgagcaat tacaaaaata ccgttcacaa gatttagcca aaaaattact tccatcaatt
     7381 gacaacctgg aacgtgcatt agccacagaa gtgtcagatg atcaaggggc aggtttgaaa
     7441 aaaggcgtgg aaatggtctt ggaaagtttg cgtaacgcac tggaagaaga aggaattgaa
     7501 aaaatccctg ctaaaggaga agcctttgat cctaatcttc atcaagcagt acaaactgtt
     7561 ccagcgactg aagatacacc agccgatacg attgtagaag tactacaaga aggctacaaa
     7621 ttacatgatc gcgtgttaag accaaccatg gtcattgttg cacaataatt gtgcggatat
     7681 aaaacttaca ctttcaatta aagaaggaga cgttaattat gagtaaaatt atcggtattg
     7741 acttaggtac gactaactct gcagtagcag tattggaagg aaacgaagca aaaatcatcg
     7801 ctaacccaga aggaaaccgt acaacacctt ctgttgtatc attcaaaaat ggagaaatcc
     7861 aagtagggga agttgcaaaa cgtcaagctg tgacaaaccc aaatacgatt tcatcaatca
     7921 aacgccatat cggtgaagca ggttacaaag tagatgtaga aggtaaatca tatacaccac
     7981 aagaaatctc agcaatgatc cttcaatata tcaaaggatt cgcagaagac tatttaggtg
     8041 aaaaagtcga aaaagcagtt atcactgtgc cagcttactt taatgatgca caacgtcaag
     8101 caacaaaaga tgctggtaag atcgctggtc tagaagtaga acgtatcgtc aatgaaccaa
     8161 ctgcagctgc tcttgcctat ggtttagata aaacagatcg cgacgaaaaa atcttagtat
     8221 tcgaccttgg tggcggtact tttgacgtat ctatccttga actaggagac ggtgtctttg
     8281 atgtattgtc aacagctggc gacaaccatc taggtgggga cgactttgat aacaaaatca
     8341 tcgactacat ggtagcagaa ttcaaaaaag aaaacagcat tgacttgtct caagacaaaa
     8401 tggctttaca acgcttgaaa gacgctgctg aaaaagcgaa aaaagattta tctggcgtat
     8461 caagcacaca aatcagcttg ccatttatca cagcaggaga atctggacca ttgcatttgg
     8521 aaatgacatt gacacgtgca aaatttgatg aattaacagc tgacttagta gaacgtacaa
     8581 aagtaccagt acgtcaagcg ctaaaagatg caggtttatc acaatcagaa atcgacgaag
     8641 ttattttagt cggtggatct actcgtatcc ctgcagttgt agaagcagta cgtaaagaaa
     8701 caggaaaaga accaaacaaa tcagttaacc ctgatgaagt agttgctatg ggtgctgcta
     8761 tccaaggtgg cgtgatcact ggtgatgtga aagatgttgt tttacttgac gttacaccat
     8821 tgtcattagg tatcgaaaca atgggtggag tgttcactaa attgattgac cgcaatacaa
     8881 cgattccaac aagtaaatca caagtattct caactgcggc agataatcaa ccagcagtag
     8941 acatccatgt attacaaggt gaacgcccaa tggctgctga caacaaaact ctaggaagat
     9001 tccaattgac agatattcca gcagctcctc gtggtattcc acaaatcgaa gtaacattcg
     9061 atattgataa aaacgggatt gtaaatgtat ctgcgaaaga tctaggtaca caaaaagaac
     9121 aaaaaatcac gatcaaatct tcttcaggtc taacagacga agaaatcgaa cgtatggtaa
     9181 aagatgcaga agcaaatgca gaagcagata aagctcgtaa agaggaagtt gacctacgta
     9241 atgatgtaga tgcattgcta ttctctgtgg acaaaacatt gaaagaatta gaaggcaaag
     9301 tggacgaaga agaagtgaaa aaagcagaaa cagctcgcga tgaattgaaa gccgctgttg
     9361 aagcaaacaa catcgaagaa atgaaaacta aacgcgatgc attgaacgaa atcgttcaaa
     9421 acttgactgt taaactatac gaacaagcgg cacaacaaca agctcaagaa aacccagaag
     9481 cagctcaagg cggagcagat gatgtcgttg atgctgattt tgaagaagtc gatggcgacg
     9541 ataaataatc agttcactga atttcgagag aaggccaaag ttgctacttt ggctttttct
     9601 tttgaaaaat agtttgaagt atcggttttt gtttcaaaca aaatatgata tgataacaag
     9661 cgacgtgaaa atgatgagaa ccaataggag gaaagcctat ggcaacaaaa cgtgattatt
     9721 atgaagtctt agggctctca aaaggagcat cagaagatga aataaagaaa gcttatcgta
     9781 agctttcgaa aaaataccat cctgacatca ataaagaacc tgatgcggaa gaaaaattca
     9841 aagaagtatc agaagcatat gagattttaa gtgacccgca aaaacgcgct gcatatgacc
     9901 aatacgggca tgcaggcaca gatccaaatt acggtgctgg tggagctggt ggcggatttg
     9961 gtggattcgg cggcttctct ggtggcggct tcggtggttt tgaagatatc ttcgactctt
    10021 tctttggagg aggcggtcgg tcagtcgatc caaatgcgcc tcgtcaaggt gctgatttgc
    10081 aatatacggt cgacttgtct tttgaagaag cgatttttgg tgtagaaaaa gaaatcaagt
    10141 ataatcggga agaaatctgt catacttgtg gtgggaacgg tgcaaaacca ggcacacaac
    10201 caactacttg tcataaatgt catggtgcag gtacgattaa tgtagaacgc caaacaccac
    10261 ttggacgagt gatgagccgt caaacttgtg atgtttgtca tgggacaggg aaagaaatca
    10321 aagaaccttg tccaacctgt catggctcag gacatgagaa gaaagcacat tcagttaaag
    10381 tgaatgtacc agctggtgta gaagaaggac aacaaatgcg tttagctggt caaggagaag
    10441 ctggagaaaa cggcggacca tttggtgatt tgtatgtggt cttccgtgta gaagaaagtg
    10501 atatctttga tcgtgaaggt tctgaaattt actatgaatt gcctttaagt tttgtacaag
    10561 cagctttggg ggatgaagtc caagtaccaa ctgtccatgg agatgtgaaa ttgaagatcc
    10621 ctgctggaac acagacaggt acgaacttcc gtttacgtgg aaaaggcgct ccgaagttac
    10681 gtggtggtgc aacaggcgac caacatgtca aagtaaaact gatcacaccg aaaaatctaa
    10741 atgaacaaca aagagaagca ttacgtgcat tcgctgaagc tggcggcatc aaagttgaag
    10801 aacagcaaga agacggtttc ttcgataagt ttaaagatgc ttttggcggg aaaaagcgga
    10861 agtagttttt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac gaacaatcat
    10921 atgttcgcct ggtttttttg tagttgtcat gtttgtacca acaaaaaagc cagctttaga
    10981 atatgataat cgtcatagtt tctaggggta taaccagtga gagaatggaa cttgttcaat
    11041 cgatattgca aggtgttttt atgaataaat aattcttctg agcaattttt gatgcttccg
    11101 ttatttttac tgaagcatgc caaagtcttc ttgaacaaat gtacttcttc ttctgtcagg
    11161 cttttcaaaa tacgttttac atataatgtt cgttgatctg acgggattgc tggtaaaacg
    11221 atcccgaggt ctaaatcttc aaaaaagcgg atctcgttca cagattgatg gaaagctatc
    11281 cattcgcaaa cttgtttggc atgaaggaaa gattgcggtg tctcttgctc tgtataacaa
    11341 agcgacccaa ttccaaagtt tagctgtaac tgatacattt tattaaactt tgtctggatt
    11401 ttatcaagca atgatagcaa tgaggctctg gaaatccgat tgactaggat aatgatttgt
    11461 tgtctatgta cagtgagcaa agcctgtccg taataagaga agaccctttc gagcaactcc
    11521 aataaaggat tgatccgttc ttgacacaat tgctgattgt cagaaagttg tcccacaact
    11581 acataatatg gagaagaaaa atcaagctca attaagtgag ggtggtatcg actatgtact
    11641 tgatatcatc aatgaaccga tggtactttc ttatgcgatg gaacaggtag agcgattgct
    11701 tgtttgtgca ggaaaacaag cgatgcggct gatacgatta ggtgagaaaa taagaagata
    11761 aaaatacacg gtcccttcat ggcgaccgtg catttttggt ttttattaaa tatttaatac
    11821 tttattcaag aaatcttttg tccgttcatt ttgcggattt tcaaagattt gttgaggcgt
    11881 tccatcttct aaaaagtttc cgtcgtcaat gaacatgacg cgattggcca cttctttagc
    11941 gaaccccatt tcgtgcgtaa cgataaccat cgtcatacct tgttctgcta actttttcat
    12001 gacgttcaag acatcaccga ccatttcagg atcgagtgca gaagtaggct catcaaaaag
    12061 catgatgtca gggttcatcg ctagagcacg agcaatagcc acacgctgtt tttgtccacc
    12121 agaaagcatt tcaggataca tatctttttt atcttctaga ccaactgtct gcagtaattt
    12181 gattgctttt ttctctgctt cttctctaga ttctttcttc aggtccatcg gtgctaaaat
    12241 gatgttttcc aaaatagaaa gatgagggaa taggttaaag tgttggaata ccattccgat
    12301 atgttgtctc accaagttga tatctgtgct tttatccatc aagtgggcgc cgtcaataat
    12361 aatttcaccg tcagaaggtt cttctaaacg attcaagcaa cgcaagaatg tactttttcc
    12421 tgaacctgaa gggccaatga tacagacgac atctccttcg ttgatggata cattgatgtc
    12481 gtttaatact gtgttatcgc cgtatttttt gactaaatgc tcaactaaga ttttttctgc
    12541 catcttactt caccttcttt tctaaaactt ttgctaattt tgtcaatgct gtgatcaaaa
    12601 tcaagtacat gatcgcaatg atcaagtaaa catacgtact ttgtgttgta cgtgcaacaa
    12661 tgatcttacc agtttgcagc aattcgacta caccgattgc ggaaatgatc gttgtgtctt
    12721 ttaatgagat aacgaactga ttgacgaatg atgggatcat gatgcgtatc gcttgcggca
    12781 aaatgatttt ttgcattgtc cgattataag aaagacctaa acttctggaa gcttccattt
    12841 gtccgacagg aacggcgttg atcccgcctc gaacaatctc tgcgatataa gcacttgcgt
    12901 taagagtcaa ggtgataaca ccagcaagga agtcaggaat cgtaaatcca acgataccag
    12961 gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg aattcctcgg atgatatcga
    13021 cataaatact agcaattgtg cgaagagcgc gtattggtgc tacactgaat aatccaaaaa
    13081 tcacaccgat tactatagcc aaagcaaagg agatcaaagt taggagaatt gttctccaaa
    13141 gtcctttcaa caatgcttga tagttgtttt ggataagtcc aagaaatgtt gattcatcag
    13201 cagtggaaga ctcactggaa gtttctgaac catccgctac atattcttta ataatcttat
    13261 cgtattcgcc tgttcttttt aattctttca atccttcatt gaacatatta agaagctcag
    13321 gattttgtcc ttttttaacg gcaaatgcat attttccgcc agattctttt ttgatcggtg
    13381 tttccaattc ttgtccttgt ttgatcgcat aaccgattac cggataatca tccatcattg
    13441 catcgattgc gccaactttt aacccgtcgt acaattgatc agcagcatca aaatatttga
    13501 tagaatagcc atattcagat tcatggtcgc ttaaaaattc agcactttcg gtaccaactt
    13561 ttgctccgac tgtttttcct ttcaaatctt cataagactt gatcgaagta tctccttttt
    13621 tgacagctaa ttggatcccg ctatcaaagt aagaatcaga aaaatcatag cttttctttc
    13681 gttcatctgt tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat tccaaagctt
    13741 gcaaagcagc agagaaacca cggtagtcca ttcggatgtt aaatccttgc agtttggcga
    13801 tgttttccat caattttaca tcgatgcctt cgtactcgcc ctttgcgttt tgaaattcaa
    13861 atggagcaaa agcagagtca ctggcaatta cgtatacgtc ttttttgggc tctatttttt
    13921 gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt gcaacatgac
    13981 caaacgcaaa tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat aaatgctttc
    14041 ttttcataat caacctccta aatttccaat attttattat tataggcgaa aagtgctgaa
    14101 tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac attttatgtt
    14161 ataaaacata acatgtataa taagataaaa aagtggcgaa agtatgttcg gtgtggtata
    14221 attttttagt aaggaatgcc tttgctttcc ttttcttttt attggtcgta aaatgaaaag
    14281 gaatgaatga aaaggaggac gaagaatgat tgagagagat acctcccgac actttgagct
    14341 tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg attaaccaat tagtggatgg
    14401 cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg actggaacag ggaaaacata
    14461 tacgatttcc aatcttattg agaaagtgaa taaaccaaca ctgattattg cccataacaa
    14521 aacattggct ggtcagctgt acggggaatt caaggaattt ttccctaaca atgctgttga
    14581 atattttgtt tcttattatg attattatca gcctgaagct tatgtgcctt caagcgatac
    14641 gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac aaattacgac attcggctac
    14701 tagttcatta ttagaaagaa atgatgtgat cgtcattgca tccgtttcat gtatttttgg
    14761 attaggctcg ccttttgagt accaaaaaca agtagtttcg attcgacagg gagcagaact
    14821 agatcgcaat cagttgattc gtgacttagt cagtatccaa ttcgaaagaa atgatataga
    14881 ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc cggcttcaag
    14941 agatgaacgt gccttacgtg tggagttttt tggagatgag gtcgaacgta tccgagaagt
    15001 caatgcactg acaggtgaag ttttaggaga aacggaacac gtagcaatct tcccggcgac
    15061 tcactttgtg acgaatgatg aacatatgga gcatgccgta gcgaatatca aagcggaatt
    15121 agaacagcgt ttaactgtct tgcgaaatga aaacaaactt ctggaagctc aacgtttaga
    15181 acagcgaacc aattacgata ttgaaatgat gttagaaatg ggctacacgt cgggaattga
    15241 aaattattct cggcatatgg acggacgtaa agaaggtgaa ccgccttata cgctattgga
    15301 tttctttcct gaagattttt tgatcgtggc ggacgaatcg cacgtaacca tgcctcaaat
    15361 cagaggaatg tataatggtg accgagcgag aaaacagatg ctagtagatt acggtttccg
    15421 tttgccatct gctttagaca atcggccact tcggttagaa gagtttgaaa aacatgtcaa
    15481 tcagatcatt tatgtctctg cgacgccagg accatatgaa catgaacaaa cagataccgt
    15541 tatccaacaa atcatccgtc cgacagggct gcttgaccca gtgatcgaag ttcgtccaat
    15601 catgggacaa atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa aagaccagcg
    15661 ggtgtttgta acgacattga cgaaaaaaat ggcagaagac ctcacagatt atttcaaaga
    15721 attagggatc aaagtcaaat atttgcatag tgatatcaaa acgctggaac gaacagaaat
    15781 cattcgagat cttcgattag gtgaatttga tgtgttagtt ggaatcaatt tgcttcgtga
    15841 aggattagac gtacctgaag tttcccttgt tgctatcttg gatgcggata aagaaggctt
    15901 tttacgtagt gaacgttcct tagtccaaac gattggacga gctgctcgga atgaagaagg
    15961 taaagtaatc atgtacgcag acaaagtaac agattctatg cgtctagcga tggatgaaac
    16021 gtcaagacgt cgtacgattc agcagaagta caatgaagaa catgggatcg ttccaaaaac
    16081 gatcattaaa gaaatccgag atttgatttc tattacgaaa gaatcggaag atgatacaaa
    16141 agaagcggta caagtctcgt acgaagaaat gacgaaggaa gaaaaagaca cgttattgat
    16201 gaaattagaa aaagaaatga aagatgcggc aaaagctttg gattttgaaa cagccgcgaa
    16261 tgtgcgagat atgattttag aattaaaagc ttcaaagtag gaggacaaca tggctaacga
    16321 taaaattacg attcatggtg cacgtgccca taacttgaaa aatatagatg taacgattcc
    16381 acgagataaa ttcgtggtcg ttacaggttt gtccggttct ggaaaaagtt ccctagcatt
    16441 tgatacgctg tacgctgaag ggcagcggag atacgtagag agcctttctg cttatgctcg
    16501 tcagttttta ggacaaatgg ataagccgga cgtagatagc atcgacggat tgagtccggc
    16561 gatttctatt gatcagaaaa caacaagtaa aaatcctcgg tcgacagtag gtactgtgac
    16621 ggaaatcaat gactatttac gcttgttgtt tgcacgtgta ggacatccga tttgtcccaa
    16681 cgatcatatc gagattacca gtcaatcagt agatcaaatg gcagatcaag taatggcttt
    16741 gcctgagcgc acaaagatcc aagtgttggc accgatcgtg gtaaagaaaa aaggacaaca
    16801 taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc cgcgttcgag tcgatggtga
    16861 gatctatgat ttaagtgaag tgcctgagct ggagaaaaac aaaaaacatg atatcgcgat
    16921 cgtcatcgac cggatcattg tgaaagaagg gattcgttct cgcctatttg attctttaga
    16981 ggcggccctg cgattagcag acggctatgc tctggttgac gtgatcggtg aagaagaaat
    17041 gttgtttagc gaacactatg cctgtcctta ttgcggattt actgttggcg aactagaacc
    17101 aagactgttc tcattcaatg cgccttttgg agcttgtcca gactgtgatg gtttaggtgt
    17161 caaattagaa gtcgatcaag atttagtgat tccagataga acgaagacac ttagtcaagg
    17221 tgctatcgtt ccatggaatc caatcagctc tcagtattat cctcaaatgt tagcgcaggc
    17281 ttgtgaaagt ttcggtatcg atttagatac accattcgaa gaattaccgg aagagcatca
    17341 ggagatcatc ttgaatggga cagatgatct tttccatttc cattatgaaa atgattttgg
    17401 tggcgttcgc gatgtagaaa cgactttcga aggggtattg aagaatatcg agcgtcgtta
    17461 tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt ctttatatga cagaattaac
    17521 ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct ctatctgttc aaatcaatgg
    17581 aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa gcagtagatt tcttctcaaa
    17641 cttaacgtta tctgaacaag agaaagtaat tgccaaaccg attttaaaag aagtaaatga
    17701 cagactttca ttcttggaaa atgtgggact agactatcta actttgagtc gcgcttccgg
    17761 aacattatct gggggagaag cgcagcgaat cagattggct acacaaattg gatcgaatct
    17821 ttctggagtt ttgtatattt tagatgaacc atcgatcgga ttgcatcaac gcgacaacga
    17881 tcgactgatt ggctcattaa agaaaatgcg tgatcttggc aacaccctcg ttgtagtcga
    17941 acatgatgaa gatacgatgt gggcagcaga ttatttgatt gacgtcggac ctggggccgg
    18001 agaacaaggc ggacaaatcg tggctgcagg cactcctgaa gaagtagcta atgatgaaaa
    18061 ttctttgaca gggaaatatc tttcagggaa aaaatcaatc cctgtaccaa aagaacgccg
    18121 taaaggtaat gggaaagcaa tcaagatcac tggtgcttca gaaaacaatc tgaaaaatat
    18181 tagtgttgaa tttccattag gagaatttgt agcagtcaca ggtgtgtctg gttctggaaa
    18241 gagtacattg gtcaatagta tcttaaagaa atcgttagcg caaaaattaa ataagaattc
    18301 tgctaagcca ggtaaattca agacaatttc cggctacgaa agtatcgaaa agatcatcga
    18361 tatcgatcaa agcccaatcg gccggacgcc gagaagtaat ccagcgactt atacaagtgt
    18421 atttgatgat atccgtgggt tatttgctca aacgaacgag gcaaaaatgc ggggttataa
    18481 gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt gaagcttgtc gcggggatgg
    18541 aattattaag atcgaaatgc actttttgcc tgatgtctat gttccttgtg aagtatgtca
    18601 tggtaaacga tataattctg aaacattaga agtgcattac aaaggaaaaa gcattgctga
    18661 tattttggaa atgacagtag aggatgctgt agaattcttc aagcacattc caaagattca
    18721 tcgcaaactg caaacgattg ttgatgttgg cttaggttat gtgacgatgg ggcaaccagc
    18781 aacgacattg tccggtggtg aggcacaacg gatgaaactt gccagtgaat tgcacaaaat
    18841 ctctaatgga aagaatttct atatactaga tgaaccaacg acaggacttc atagcgatga
    18901 catcgcccgc ttgttgcatg tattacaaag attagtagat gctggtaaca cagttttagt
    18961 gattgaacac aatctagatg taatcaaaac agcagattat atcattgatt taggaccaga
    19021 aggtggagaa ggtggaggaa cgatccttgc gactggaaca ccagaagaaa tcattaacgt
    19081 aaaagaaagt tatacaggtc actatttgaa aaaaataatg gtataaagag gatagatagc
    19141 tgagatagtt aaaaaaacat aaaaaaatga atttttgctt gttttattaa tatcagtgtt
    19201 ctagaatcat tacatggtag ttctttctac actgattggt atagataaaa acttcctata
    19261 agttcaatcc gttcaaaaaa gaattgagca atcggaggac tttcctttga ttgctttttt
    19321 ctttatggta tgatattcct tgtgtagttg gaaaagaaaa acaagagatt atgagtaagt
    19381 gagagataat gatgaacagt attgaaaatg aagaatttat ccctatatta ttaggaagcg
    19441 acatgaacgt ttatggtatg gcacgttctt ttaatgaagc atacggcaaa atctgccaag
    19501 catatgcttc ggatcagtta gcccctaccc gctacagtaa aattgtcaat gtagaagtta
    19561 ttccaggatt tgataaagac cctgtattta tcgagacgat gcttcgtttg gcaaaagaaa
    19621 gatattctga taagagtaaa aaatatcttt tgattgcttg tggtgatgga tatgcagaat
    19681 tgatctccca acataaacaa gagctgtcag aatattttat ctgtccatat attgattatt
    19741 ctttatttga aaggctgatc aataaagtaa gcttttatga agtttgtgaa gaatatgatc
    19801 ttccgtatcc taaaacattg atcgtcagag aagaaatgtt agtaaatgga catttggaac
    19861 aggaattgcc ttttgaattc ccagttgctt tgaaacctgc aaacagtgtg gagtatcttt
    19921 ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga gactagagaa gagttcgatt
    19981 taattttagg ccggatatac gaagccggtt ataaaagtga aatgattgta caagatttta
    20041 tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta tgtagatgaa gatcatcaag
    20101 ttcggatgat gtgccttggt catccactat tagaagatcc tacaccagca tcgattggta
    20161 attacgttgt gatcatgcca gattataacg aaaaaatcta tcaaaccatt aaggcatttt
    20221 tggaaaaaat cgaatataca ggctttgcca atttcgatat gaagtatgat cctagagatg
    20281 gagaatataa attatttgaa atcaatttga gacaaggtcg tagcagtttc tttgtgacat
    20341 tgaacggctt gaatctggct cgttttgtca cagaagatcg agtgttcaac aaaccctttg
    20401 ttgaaacgac ctatggaacg aatcaatcag ataaagcaag attatggatg ggtgtgccca
    20461 aaaaaatctt tttagaatat gctcgggaaa atgaagacaa aaaactagct gagcaaatga
    20521 tcaaagaaaa tcgttacggt actactgtat tttatgaaaa agaccgatca atcaagcgct
    20581 ggttgttgat gaaatatatg ttccataact atattccaag attcaaaaag tattttcatg
    20641 tgaaagaagg ctagttcatg gagaattttt tcagtatttt aggcggaatg ggcacgatgg
    20701 cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa agctacaaaa gatcaagaat
    20761 atttgaatta tgtcttattc aatcatgcaa cagttcctga tcgtacagct tatattttag
    20821 atcgatcaga agaaaatcca atgccgtttc tgctggatga tattgagaaa caaaatttat
    20881 tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca ctactttttt gaagagttac
    20941 aagcggcaac ggatattcct atcttgcata tgccaagaga agcagcaaat gaacttgtcc
    21001 gtcagcatac gacagggaga gtagcgattc taggaacaga aggaagtatg aaagctggaa
    21061 tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt gattcctgat actgcgcttc
    21121 aagaaaaaat caattacctg atctatcatg agatcaagga atcggattat ttgaatcaag
    21181 agctctatta cgaaatctta gaggaagcgg tagagcgcct aaactgtgaa aaagtcatcc
    21241 taggttgcac agaattatct ttgatgcatg aatttgctga agataatcat tacccagtga
    21301 tcgatgctca atctatttta gcggatcgaa cgatcgaacg tgcattagct gaaagaagcg
    21361 aggcattgga tacagcatcg gaaaagtaaa aaaattggat ttggggcaaa tatcctaaaa
    21421 gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac tggtaatgat acgcccggga
    21481 tgatataatt ttattatgca caatgtgaga ggggttcgta aatatgacag ataatttgca
    21541 attagtcatc ataacaggta tgagtggtgc agggaaaaca gtagctatcc aaagttttga
    21601 agatatgggc tatttttgta tcgataatat gccgcctcga ttgattccaa aattctggga
    21661 attgatcaaa gaatcaggta aagtcacaaa aatggcttta ggagtggatt tgcgttctcg
    21721 ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc gaaaatacga acttcatcga
    21781 cacttctatc ttatttttag atgcttcaga cgaagaacta gtggctcgct acaaagaaac
    21841 aagacgagca catccaatgg cgatggacgg tttgatcact gaagggatac gtaaagaacg
    21901 ggcgatacta gacgacttga aagcccaagc ttccgtgatc attgatacga caacattgac
    21961 accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa tcaaagggag accaaggatt
    22021 tcggatcgaa tttgtatcat ttggttttaa atacgggttg ccgatcgatt cggatattgt
    22081 gatggatgtg cgttttttgc ccaaccctca ttatatcccg gaactaagac cattgaccgg
    22141 aaaagatcag ccagtctatg attatgtgat gtcctttgat gaaacgcaat cattctacca
    22201 taaatttttg gatcttctgg aaactgtatt gcctggatac attaaagaag gaaaaagcag
    22261 cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt tctgtggcat tgactgaacg
    22321 tttggcaaat gctttaggga aaaactataa agtcaatgtg acacatcggg acaaggataa
    22381 aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct accggataag aaaaccaaag
    22441 atcgtagtgg tcggcggtgg cactggtctg ccagttatcc tgaaaagttt acgaaatcaa
    22501 ggagcagata tcacagcagt agtgactgtt gcagatgatg gtggaagcag cggtgcaatc
    22561 agagaatcga tcgctatggc tcctcctggt gacttgagaa atgtcttagt ggcgttatcg
    22621 gatatgccac aattctatga agatattttc caataccgtt tcaaaaagga agatcagttt
    22681 ttagccaatc atacgatcgg taatctgatc attgccgcag tttccgagat gcggggaagc
    22741 acttatgagg cgattcaatt attagcaaaa atgatgcatg tagatggaca tgtttatcct
    22801 tcttcagaaa ctcctttgac cttgcatgct gtttttaaag acggaacgac tgcagtagga
    22861 gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg tgtatgtctg caacacaaat
    22921 gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag caattatgga cgccgacatg
    22981 gtcgttttgg gacccggaag tttattcacc agtatccttc caaatttagt gataccagaa
    23041 atcggcgaag caatgctcaa gacaactgct gaaaccgtct atatctgtaa tatcatgacc
    23101 caaaaagggg aaacggaaca tttcacagat gccgatcata tacgtgtttt acacaagcat
    23161 ttgaatcagc cttttatcga tacagtattg gtcaatacgg aaaaagtgcc tgaagattat
    23221 atggacccag agatctatga tgagtatcta gtacaagtcg cacacgactt caacggattg
    23281 agaaatgaag gatgtcgagt gatttccacc gactttttag aattgaaaga tggtggtgtc
    23341 ttccacgatg gagacaaagt agtagaagaa ttgttccgtt tagttttcgg ttcaaaatat
    23401 taagcaattt attgttggag aaaggaggag aataggatgt catttgctgc agatgtgaag
    23461 aaagaactaa ctggtttgga agttcatcga gaacatgcga aagcggaatt agctgctttg
    23521 ctgcgaatga acggctcttt aggtattgtg aaccagcagt ttattttgaa cgtccaaaca
    23581 gaaaatgctg caattgccag acgtatctat tctcttttga aagatcatta tcaggtacga
    23641 agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga acaatgtcta tatcgtccgg
    23701 ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata tcatggatgg tatgatgttc
    23761 aatggacacg tttcggacga gattatggga aattcgcaaa aaatgcgttc ttacttacgt
    23821 ggtgctttta tggcctcagg ttcagtcaat aatccggaaa cgagtcgtta tcatttggaa
    23881 atattttcta tttatgaaca gcataaccaa gacatttgcg atatgttaaa ttattacggt
    23941 ctacatgctc gaacgcttga acggcgcaat ggctatattt cgtatttaaa aggagcagaa
    24001 aaaattgctg atttcttaac attgattggt gcaaccaact caatgctccg atttgaagat
    24061 gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc tagtcaattg tgagacagca
    24121 aatctgaaca agacgattga tgcggcttct aaacaaatcg aaaacatcga attgatcgaa
    24181 gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg aaatcgcaga acttcggctt
    24241 caacatccag aggtcagctt gaaagaatta ggggaaatga ttccctcagg tgcaatatca
    24301 aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat ttgcggaaaa actgcgtgaa
    24361 gaagcagttt aatccaaaga aaaaactgat acatgaagca ggagatgcga atgtatcagt
    24421 tttttttaaa agctatactt gcttttcttc cgtcaaacta taattccaac tttactggag
    24481 taacataatg ttgaaaggct tctattgttt tttaatacaa tcgtataaaa agcggggaga
    24541 caactgaatg aagattttag ttttatacat aaacttttgc ttaatataga agtcctgata
    24601 tagataggat caagtattta tctataactg gagattaatt aaaaactgga aggaactgct
    24661 cgatcaacct tgagaactgt ttttcttact tctggtcaag attctcgtgg ttggtggata
    24721 aggatggttg ataagttcat tggattagct agtcgaaaac aaccaggtgt aattaatgta
    24781 gaagttttaa caaggggatg gagtgattta ttcagtgata atcataagtt cataaatagt
    24841 aattgggatg tatggttggt atacgttata cctgaacttg aactatcaag atttggttta
    24901 gaatttgaga atcgatgttt ataaagggag ggcaaaaaat gtgctttttt acaactttta
    24961 ctatcattat gattgcagta ctttttattt ttctagatat agcaaagcga aatacagcat
    25021 ttttattata tcgggtttta cttcgagcag gtctcataac atttatatcg atcgttggat
    25081 tttttctatt cacagttatt gtatttattt ggagaactac agcaccacca ttgccagaga
    25141 ttacatatgg agaatttccg tttagtcttg aatatgaaat agatgaggaa agaattttga
    25201 ttgaagatac ggtgattgca gaatttatag gaagttttag agggaatgtg acaactcaac
    25261 ctagacgaag ttggagtata aaacttttaa ataatagtgg agagttgaaa gcatttcatt
    25321 atgaattctt aataatagaa agtgaagatg taacaatcac tttaagtagt ggctctgctg
    25381 gatattttat gaatgatggt ggaagcaagc cacagctcac agtccgtgat cgtattgcag
    25441 gttttatgca tttttctcac tatgacttag aagaagctcg taaaatactt gcagaatatg
    25501 ggatcacatt aatcaattgg gaatatgcgc caccgataag aaattttttc aatgattatt
    25561 attagtctta taaaaaacag aatgtaaaat ttatgtgcgc gagtatttag ttttccattt
    25621 tttgtttgat agatatatag cattgttgaa gcatgggata gtggtgccaa ttttatcaat
    25681 gaggttggtc aggcagttaa cataattggt gatgctataa atccatttaa ttggaattgg
    25741 aggaattagg tgtggaaaca gtgagattat ggatattcat gaaaaatttt cgttcggctt
    25801 tgagatatgt tattctggaa tcaaaatcag gttattatct gattgacaca gatcactata
    25861 ctttttgggg aggctacctt tttccgattt ttaattggat aatcccccat aaggttatga
    25921 aaattaactt gcctgcagaa gaaataaata gattaatact caacaaagat ttacaaagga
    25981 aaagagagaa agaacattct aatgttgctt ggataggtcc agcatttgca gtagtgactt
    26041 cttcaattgt cggtcctatg ctagagagta tttatagtga tttttctatt ggaatacgtt
    26101 atttatttgt tactttaagt atagctgttc ttttgtgcat aaagggttat gttagcaaga
    26161 agaataaaga tattttaaca ctaatcggtg taaacaattt atctagtact aaaattatac
    26221 tgtttcctga atctataaaa gaaggatcaa ggttgttgat aatttttata atgcttatac
    26281 tgttttctgt tttatttggg gtgatgttta ta
//
LOCUS       DAZBTH010000028        25820 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000028 DAZBTH010000000
VERSION     DAZBTH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.028"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..2220
                     /locus_tag="ACHGNW_001154"
     CDS             160..2220
                     /locus_tag="ACHGNW_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN6925482.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2303..2872
                     /locus_tag="ACHGNW_001155"
     CDS             2303..2872
                     /locus_tag="ACHGNW_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925483.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3047..3853)
                     /locus_tag="ACHGNW_001156"
     CDS             complement(3047..3853)
                     /locus_tag="ACHGNW_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925484.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3991..5505
                     /locus_tag="ACHGNW_001157"
     CDS             3991..5505
                     /locus_tag="ACHGNW_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN6925485.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5908..6891)
                     /locus_tag="ACHGNW_001158"
     CDS             complement(5908..6891)
                     /locus_tag="ACHGNW_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN6925486.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6896..7543)
                     /locus_tag="ACHGNW_001159"
     CDS             complement(6896..7543)
                     /locus_tag="ACHGNW_001159"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6925487.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7628..8260)
                     /locus_tag="ACHGNW_001160"
     CDS             complement(7628..8260)
                     /locus_tag="ACHGNW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6925488.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8276..8827)
                     /gene="coaC"
                     /locus_tag="ACHGNW_001161"
     CDS             complement(8276..8827)
                     /gene="coaC"
                     /locus_tag="ACHGNW_001161"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN6925489.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8824..9561)
                     /locus_tag="ACHGNW_001162"
     CDS             complement(8824..9561)
                     /locus_tag="ACHGNW_001162"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN6925490.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9558..10547)
                     /locus_tag="ACHGNW_001163"
     CDS             complement(9558..10547)
                     /locus_tag="ACHGNW_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN6925491.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10805..11347
                     /locus_tag="ACHGNW_001164"
     CDS             10805..11347
                     /locus_tag="ACHGNW_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN6925492.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11364..12449
                     /locus_tag="ACHGNW_001165"
     CDS             11364..12449
                     /locus_tag="ACHGNW_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925493.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12461..13267
                     /locus_tag="ACHGNW_001166"
     CDS             12461..13267
                     /locus_tag="ACHGNW_001166"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925494.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13268..14095
                     /locus_tag="ACHGNW_001167"
     CDS             13268..14095
                     /locus_tag="ACHGNW_001167"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925495.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14092..15165
                     /locus_tag="ACHGNW_001168"
     CDS             14092..15165
                     /locus_tag="ACHGNW_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6925496.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15593..18289
                     /locus_tag="ACHGNW_001169"
     CDS             15593..18289
                     /locus_tag="ACHGNW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6925497.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18354..18854
                     /locus_tag="ACHGNW_001170"
     CDS             18354..18854
                     /locus_tag="ACHGNW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6925498.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18866..19360
                     /locus_tag="ACHGNW_001171"
     CDS             18866..19360
                     /locus_tag="ACHGNW_001171"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6925499.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19381..20190
                     /locus_tag="ACHGNW_001172"
     CDS             19381..20190
                     /locus_tag="ACHGNW_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6925500.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20187..21008
                     /locus_tag="ACHGNW_001173"
     CDS             20187..21008
                     /locus_tag="ACHGNW_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6925501.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21059..23158
                     /locus_tag="ACHGNW_001174"
     CDS             21059..23158
                     /locus_tag="ACHGNW_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN6925502.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23155..24273
                     /locus_tag="ACHGNW_001175"
     CDS             23155..24273
                     /locus_tag="ACHGNW_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6925503.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24312..25190
                     /locus_tag="ACHGNW_001176"
     CDS             24312..25190
                     /locus_tag="ACHGNW_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6925504.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25336..25533
                     /locus_tag="ACHGNW_001177"
     CDS             25336..25533
                     /locus_tag="ACHGNW_001177"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6925505.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25517..25747
                     /locus_tag="ACHGNW_001178"
     CDS             25517..25747
                     /locus_tag="ACHGNW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925506.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8167 a         4479 c         5441 g         7733 t
ORIGIN      
        1 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
       61 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
      121 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
      181 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
      241 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
      301 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
      361 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
      421 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
      481 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
      541 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
      601 catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa
      661 gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata
      721 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
      781 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
      841 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
      901 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
      961 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     1021 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     1081 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     1141 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     1201 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     1261 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     1321 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     1381 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     1441 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     1501 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     1561 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     1621 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     1681 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     1741 aaagaacaaa aaagacgaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     1801 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     1861 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     1921 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     1981 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     2041 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     2101 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     2161 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     2221 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     2281 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     2341 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     2401 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     2461 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     2521 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     2581 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     2641 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     2701 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     2761 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     2821 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     2881 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     2941 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     3001 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     3061 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     3121 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     3181 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     3241 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     3301 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     3361 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     3421 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     3481 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     3541 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     3601 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     3661 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     3721 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     3781 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     3841 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     3901 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     3961 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     4021 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     4081 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     4141 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     4201 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     4261 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     4321 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     4381 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     4441 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     4501 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     4561 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     4621 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     4681 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     4741 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     4801 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     4861 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     4921 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     4981 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     5041 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     5101 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     5161 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     5221 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     5281 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     5341 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     5401 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     5461 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     5521 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     5581 tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa
     5641 aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaac
     5701 accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     5761 aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa
     5821 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
     5881 ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat
     5941 gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg
     6001 gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt
     6061 ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg
     6121 gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat
     6181 accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga
     6241 agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc
     6301 ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa
     6361 ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc
     6421 taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc
     6481 ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat
     6541 tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat
     6601 cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt
     6661 aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat
     6721 agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc
     6781 tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat
     6841 ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt
     6901 cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta
     6961 gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag
     7021 agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt
     7081 cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca
     7141 gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg
     7201 ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag
     7261 ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag
     7321 agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg
     7381 accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag
     7441 caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt
     7501 gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga
     7561 cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg
     7621 atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg
     7681 ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta
     7741 gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa tcttgctgga
     7801 ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg gaaagtccag
     7861 ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga cctttataaa
     7921 cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc aaggctttcc
     7981 agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa gacaaaggtg
     8041 tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca agaaaagcac
     8101 ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg gttgttgcat
     8161 tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc aaaatagcta
     8221 aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct tttttctaag
     8281 catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata tcagctaagg
     8341 caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt tcctgatagc
     8401 cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac atattcgtgt
     8461 tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg actgttgaaa
     8521 gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta gcaggcgcga
     8581 tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc ggatttgatt
     8641 cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga
     8701 taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat ttgacgaatt
     8761 gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct agtaagatct
     8821 ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa tctcagattt
     8881 actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt gtgtctcgcc
     8941 atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta tcagactatt
     9001 ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga aaccaatgat
     9061 ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt tcttcaaaac
     9121 gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg tctgttcatt
     9181 ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt tttttgccag
     9241 ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac tgtggataat
     9301 cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt ccagtgcaga
     9361 ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt gtttcgtagt
     9421 cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg cttgccctaa
     9481 tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag tcgttcctcc
     9541 agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct tccttggtgt
     9601 gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat ctcttggacg
     9661 accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag acgttgatct
     9721 attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat aaaaggcaag
     9781 acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc accgttataa
     9841 gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg
     9901 atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg tcgtacgact
     9961 tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga aaaggcaatg
    10021 attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac accgccagaa
    10081 gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa agcgttcaca
    10141 cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag cgtcatattt
    10201 tttggtagag gaactaacca gtctcctggt actttttgat attggctata accgcctgga
    10261 tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga aaaacgtgga
    10321 ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg atatgttcgt
    10381 atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc agaataggca
    10441 atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact gattgctgta
    10501 tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg tgagacactc
    10561 cttttaaaat tttcttttat cataccataa agcctactct tttattcttg ttcttccgta
    10621 aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt attccaaaag
    10681 ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa taaatttcgt
    10741 atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg attcggagga
    10801 aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt tgacacaaga
    10861 agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg aacgagattt
    10921 aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg tgactccaga
    10981 agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag aagatcatac
    11041 ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg attcgaatga
    11101 aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata agacgtttga
    11161 accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt taagcttagg
    11221 agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga caaaacaaca
    11281 tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa cgcaatcata
    11341 tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat caagtagtga
    11401 aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat
    11461 tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt attatagctg
    11521 gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg aatgatttgc
    11581 cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt cctcatatga
    11641 atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa tcagatatcg
    11701 aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga
    11761 ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca attgtaaatg
    11821 aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag
    11881 atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt atttttgtta
    11941 ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg aataaaggga
    12001 aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat catttcgttg
    12061 ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat aatctagttg
    12121 agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg
    12181 aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga aaactagtcg
    12241 taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt tatgatgaac
    12301 agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc aaagtaggat
    12361 tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg
    12421 atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat
    12481 ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg tattaatgat
    12541 catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt atctatctta
    12601 ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg catttttgat
    12661 cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa aactgaagca
    12721 caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc tgcttaaagc
    12781 ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct ttgattttat
    12841 cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg tggcaacgta
    12901 tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga tgaatccttc
    12961 tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc gacgagtgat
    13021 ttttccgtta ttattaaatg gtgtgaaaag tggtgtccaa gcagtattta ttccttctct
    13081 ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt taggaacggc
    13141 tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat
    13201 tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg
    13261 ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct ttttattctt
    13321 atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa cgatgaacag
    13381 ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac gtttgatcat
    13441 tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca cgatcatcgg
    13501 gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga cagcgttgtt
    13561 gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta gtttcttgat
    13621 tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt cccatattgc
    13681 tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc
    13741 aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca aaaatatcat
    13801 tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta cctattctct
    13861 tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc tctcagtaga
    13921 gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt
    13981 tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag aaaaccgtcc
    14041 aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa
    14101 ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct tggtgttcgt
    14161 caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta taactgggga
    14221 gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata caaagtgaat
    14281 tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac
    14341 tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga aaaactagta
    14401 cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg atttttagac
    14461 ttggattttg atccagataa tcagtattcg attccatatt tttggggaac tttagggatc
    14521 gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga tttgtggaag
    14581 ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat ggggctttct
    14641 ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagttgac agaagcagca
    14701 aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga gatcaaaatg
    14761 tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc tgccgatatg
    14821 atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa tctttggttt
    14881 gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga tttcatcaac
    14941 tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta ttctacacca
    15001 aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca attctatcca
    15061 tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa atacctaggc
    15121 atctataatg acctattctt agaattcaag atgtatagga gataatggca aaactcagta
    15181 agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct atctgagttt
    15241 tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt tattttttct
    15301 accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg cccgagacgg
    15361 gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa actatgcaaa
    15421 tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag ttcaatattt
    15481 gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta gataaattat
    15541 tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa aattggaata
    15601 caaatataga atagacatat tgcagccgat agtttattat gcttttagac acattaaact
    15661 ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac
    15721 agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca
    15781 gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg
    15841 aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat
    15901 aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg tcattcaaaa
    15961 atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac
    16021 gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc atgagcaaat
    16081 tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg acaatcccga
    16141 actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg ctgaaaagga
    16201 taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg aggtccatcg
    16261 cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa actatcgtcc
    16321 agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg ccacgacgga
    16381 aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta gtgttatgtt
    16441 gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat tattttttca
    16501 aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct tgtctttaat
    16561 gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg
    16621 tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa atgagatctt
    16681 gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat
    16741 aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt
    16801 ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt cttcgataaa
    16861 gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag
    16921 aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg
    16981 attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag
    17041 ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac
    17101 ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg acgagaactt
    17161 tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa
    17221 gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca
    17281 attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga
    17341 gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct
    17401 gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg atttaaaagg
    17461 ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg gcttgaagat
    17521 gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa
    17581 tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct gtatgtcggg
    17641 tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg atgaatccat
    17701 cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa atgatgaaac
    17761 ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat
    17821 tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt tggagcatat
    17881 tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc tagtaaaatt
    17941 cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga tcgtgatgag
    18001 tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt taaatggccg
    18061 tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat tcttggctcg
    18121 gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg aattttatta
    18181 tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta
    18241 tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa
    18301 aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt aacgtgatta
    18361 aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag
    18421 gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct tcaggctttc
    18481 ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt aatgcttatg
    18541 tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg aacgagcagg
    18601 atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag attaccaaaa
    18661 cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg ccaattctgc
    18721 tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa ctggtaaata
    18781 aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta aatgaagagg
    18841 aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg aacgattaat
    18901 tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt tagtagtagc
    18961 aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg ctgcaccatc
    19021 gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa aagatccgcg
    19081 ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg taaaagttgc
    19141 gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga aaatgacacc
    19201 gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca
    19261 agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta caccggcgat
    19321 ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag gagaaaaatt
    19381 atgtatgatg catttgtagt cgctttggcc gtttttattg gaattgctgg acatgaaatg
    19441 ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg agcgttgatg
    19501 ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt tatgggtgtc
    19561 gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct ggctacagcg
    19621 ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt tccactagca
    19681 gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc gatggctgct
    19741 aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct gcacttcttt
    19801 atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt tttatttgga
    19861 gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa cggattaaca
    19921 gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat gctttggagt
    19981 aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct cgagttacca
    20041 ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca aagagatcta
    20101 caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt
    20161 gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct aaagatgaga
    20221 aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg gtgaatccgg
    20281 tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat catttctaca
    20341 aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt tcgacgacca
    20401 ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa aattctgaaa
    20461 aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg ggaccattgg
    20521 ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg ggactagcga
    20581 tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt atgttcaata
    20641 tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta ggctctaaat
    20701 atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct gctagtatct
    20761 tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat accgttttga
    20821 gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat agtattttta
    20881 aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat aaaaaagtcg
    20941 gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca ttacttggaa
    21001 tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga gggaaaagat
    21061 gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg agttaaacga
    21121 cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg atgtaaaggg
    21181 attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt gcgggacgat
    21241 tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag gtgtttgggt
    21301 caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa acgggattgt
    21361 ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag actgtttgcc
    21421 tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg ctcccatact
    21481 ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg aaacacacga
    21541 aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca
    21601 aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg aagtggcaag
    21661 cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc
    21721 tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat ttgggcagct
    21781 gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc aaagcttttc
    21841 tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca gctatcctgc
    21901 tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca ccatgtttga
    21961 accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta agtttcctgg
    22021 ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca gtgcgttact
    22081 ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt gtccgcaggt
    22141 tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt caggggaacc
    22201 aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt atcataccgg
    22261 cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg aagccctaga
    22321 acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg aatcaatttg
    22381 taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa tcggtgatga
    22441 ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc aagggttaat
    22501 ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg atggagaaga
    22561 atcagacact acgttgatgc ctttttatca atttttagat aataaagatc atttatatca
    22621 gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg aaatcaaagg
    22681 catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg ttttaatgag
    22741 cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt gtttggcaga
    22801 tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag gagaagtcgt
    22861 gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca gcctaatgat
    22921 cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc ctgttgataa
    22981 tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac
    23041 gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa
    23101 aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa aaagatgagt
    23161 atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac tcccgaaaat
    23221 ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc aagccgcaaa
    23281 ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga tattactgga
    23341 acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt tggcagtttt
    23401 gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc tgctgttgaa
    23461 agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt gaactcgttg
    23521 ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc tttccaaaca
    23581 tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt tttttatacg
    23641 ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca cgcaaattgg
    23701 catcgggaac gcattaccaa attacaggag gattataagt taattgatcg attaaagggt
    23761 caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta ttggtggggc
    23821 gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt aacctcaaca
    23881 ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga gttcggacgt
    23941 ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc aaatagagat
    24001 gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac
    24061 gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg ttttaccgaa
    24121 gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa
    24181 gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa tactaaacgt
    24241 catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat attcgaaggg
    24301 aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt gtctgtgcag
    24361 tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca actccagttg
    24421 aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg gcaggcatcg
    24481 gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc ggtcatatca
    24541 catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta aagcaatatt
    24601 tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt gaatatgttg
    24661 ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca ggtatcggag
    24721 gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa atggggcata
    24781 tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc caccaagatt
    24841 gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga
    24901 atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat
    24961 acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt gtaatgaaac
    25021 aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat ggatatgttg
    25081 aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat gccggaataa
    25141 tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa ttaatgaatg
    25201 tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca aatgaagttg
    25261 gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt gttttcatta
    25321 aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc gctgtatacc
    25381 ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta ctttatgtct
    25441 gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt tgacttcata
    25501 atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt ataaaataaa
    25561 tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt
    25621 ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag aaattctata
    25681 tttaccttta actctcgctt ctcttgcaaa tgtagttagc attttaatcg ataataaact
    25741 caggtagtta tattaccttg aaatgattga taattttgtc acttctattt tttttgcaat
    25801 aaatttttta ttttagtgaa
//
LOCUS       DAZBTH010000029        25500 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000029 DAZBTH010000000
VERSION     DAZBTH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.029"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..705
                     /locus_tag="ACHGNW_001179"
     CDS             151..705
                     /locus_tag="ACHGNW_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925507.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(878..1315)
                     /locus_tag="ACHGNW_001180"
     CDS             complement(878..1315)
                     /locus_tag="ACHGNW_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925508.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1363..2310
                     /locus_tag="ACHGNW_001181"
                     /pseudo
     CDS             1363..2310
                     /locus_tag="ACHGNW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2581..3016
                     /locus_tag="ACHGNW_001182"
                     /pseudo
     CDS             2581..3016
                     /locus_tag="ACHGNW_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3032..3865
                     /locus_tag="ACHGNW_001183"
     CDS             3032..3865
                     /locus_tag="ACHGNW_001183"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6925509.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4110..5135
                     /locus_tag="ACHGNW_001184"
     CDS             4110..5135
                     /locus_tag="ACHGNW_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6925510.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5273..5398
                     /locus_tag="ACHGNW_001185"
     CDS             5273..5398
                     /locus_tag="ACHGNW_001185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925511.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5423..6538
                     /locus_tag="ACHGNW_001186"
                     /pseudo
     CDS             5423..6538
                     /locus_tag="ACHGNW_001186"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6621..7517
                     /locus_tag="ACHGNW_001187"
                     /pseudo
     CDS             6621..7517
                     /locus_tag="ACHGNW_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7533..8357
                     /locus_tag="ACHGNW_001188"
     CDS             7533..8357
                     /locus_tag="ACHGNW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925512.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8389..9699
                     /locus_tag="ACHGNW_001189"
     CDS             8389..9699
                     /locus_tag="ACHGNW_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN6925513.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9847..11106
                     /locus_tag="ACHGNW_001190"
     CDS             9847..11106
                     /locus_tag="ACHGNW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6925514.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11116..11988
                     /locus_tag="ACHGNW_001191"
     CDS             11116..11988
                     /locus_tag="ACHGNW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925515.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12000..12830
                     /locus_tag="ACHGNW_001192"
     CDS             12000..12830
                     /locus_tag="ACHGNW_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925516.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12870..15053
                     /locus_tag="ACHGNW_001193"
     CDS             12870..15053
                     /locus_tag="ACHGNW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6925517.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15114..16334
                     /locus_tag="ACHGNW_001194"
     CDS             15114..16334
                     /locus_tag="ACHGNW_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6925518.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16331..17791
                     /gene="gtfA"
                     /locus_tag="ACHGNW_001195"
     CDS             16331..17791
                     /gene="gtfA"
                     /locus_tag="ACHGNW_001195"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN6925519.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17868..18974
                     /locus_tag="ACHGNW_001196"
                     /pseudo
     CDS             17868..18974
                     /locus_tag="ACHGNW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19296..19505
                     /locus_tag="ACHGNW_001197"
     CDS             19296..19505
                     /locus_tag="ACHGNW_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6925520.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19498..19647
                     /locus_tag="ACHGNW_001198"
     CDS             19498..19647
                     /locus_tag="ACHGNW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN6925521.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19801..20466
                     /locus_tag="ACHGNW_001199"
     CDS             19801..20466
                     /locus_tag="ACHGNW_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925522.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20750..21835)
                     /gene="xerS"
                     /locus_tag="ACHGNW_001200"
     CDS             complement(20750..21835)
                     /gene="xerS"
                     /locus_tag="ACHGNW_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN6925523.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22169..22402
                     /locus_tag="ACHGNW_001201"
     CDS             22169..22402
                     /locus_tag="ACHGNW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN6925524.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22399..22806
                     /locus_tag="ACHGNW_001202"
     CDS             22399..22806
                     /locus_tag="ACHGNW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6925525.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINPIKTHTQPI"
     gene            complement(23943..24461)
                     /locus_tag="ACHGNW_001203"
     CDS             complement(23943..24461)
                     /locus_tag="ACHGNW_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN6925526.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24828..25076
                     /locus_tag="ACHGNW_001204"
     CDS             24828..25076
                     /locus_tag="ACHGNW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925527.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8679 a         3897 c         4756 g         8168 t
ORIGIN      
        1 gaatattctg agttactgaa atgatataaa atcataaact aaatcccttg attttattga
       61 ctttcaaaag tttatatata taagttaatg attatggtaa aatatataca taatcattaa
      121 ctcagaatat ttttaagaag ggaaacaata gtgagctata atgtacaacc gttaaggaca
      181 aatgaagaga tacaagattt tcagttttgg ctaagaagaa caagtaatcc ggaaagagat
      241 agttttttat ttttgtttgg aatcaataat gggttacgta tgtcagatat tataggatta
      301 aaagttggag atatacgagg caaaagtaag cctattattg ttgaacataa aacaggtaag
      361 cgaaaaccga tttttataga taatttgaga gaagaaattt tattatatac tgaaggtaaa
      421 gaagaaaatg attggttatt tccgagtcgg caacaaggtc gacatataac aagggatcgt
      481 gtctatcaga tatatgcaga tatagcagaa aaattaggac gagatgatat aggaacacat
      541 acattaagaa aaacttttgg gtatcactac tacaagaaaa ctagagacat tgctacctta
      601 atgtttattt ttaatcatag ctcgcaagca attacaaaaa ggtacattgg tataactgaa
      661 gatgaaatag gagcatcact tcgtggattt aaactaggtg tataatttaa aaatttttat
      721 aaacagtaaa gacaaagatt aaaagctatt tttatctaga acgattttat ttcataaagt
      781 tcgcggaata aaagctgggg ttactggtac ttggtatagg ttcgtgttat ataaactaag
      841 taattaaata acatttttag gttggaaaag gatgaaatta aaaaaagaga atcaatacgg
      901 tctcaaaaac gcaaacgctg cggaataaga aaaatactga taactcaacg tttagcagac
      961 tatctaatag ggatagtctg cttttttcgt ttttgaccat acttttgacc ttctgcaaac
     1021 ttccttgctt ttcctctgta gaactcatga aatctgcaaa acgaattgcc gtttcctctg
     1081 tctgcttttt cgcgatatgg gtgtatatat catccatagt ggttttaata tctgtatgtc
     1141 caagtcgaac atacacttct ttgacagacg caccagattc aaagagcaga ctacaatggg
     1201 tatgacgaaa accatgaagt gtgatatgtt ttaagttatt ctctttaatg atgattttca
     1261 aattgtgatt tacatagtct aaataaaggg gcttattttc ttctgatgaa aacaaaagtt
     1321 gttcaccatc ttttataggt ttgattctca tttttagata ttatgcccgc acgtattcat
     1381 cacatttcta ttgtgaatcg ctttattcga ccgacatttg acttttatca taatatactc
     1441 ggtttaaaat tactaatgaa aacaattaat caagacgatc ataccatgta tcacctgttt
     1501 ttttcagata atcaccaccg tgtgggaact gaactgactt tttttgaagt acaagaaggg
     1561 aacaaccagt tttttggaac caatacgatt gaacgaacga tactaaaagt tccttccgaa
     1621 gcatcattgc atttttggga aatatatttt gaaacacaag gcgtttgcca ttatggaatc
     1681 gaatcatttt ctggacgacc tatcttacgt tttgaagggc cagatgcaac acgattagct
     1741 ttagttccct tacgcgaatt tgaagatatt gatgattatt tcccgtatgt cacagaccag
     1801 atccctacag agcatgccat tttaggaatt gatgccattc aactacgcgt acaatattcc
     1861 gatgcgactg aacgagagtt attgtcggta ggctggtagc acaaagaata cgtcagtttc
     1921 ttttcgggtc atcaacgagt gacaatctta gaaaatcaag acactcagtt ctatcaagaa
     1981 gttcagatta ttcaagatcg tgagaatcca gttgccattg aagggattgg tagcgtccat
     2041 catgttgcgt ttggcgtaga aaataaatct gatttacaac gcatcgacaa acaattacaa
     2101 gagcgcaatt ttataaattc aggaataaaa gatcgtgaat tttttgtatc actttattat
     2161 cgtgacccaa atcaattatt gattgaaatt gctacagggg aaggaaacct agatgcaaaa
     2221 gcgtatgaaa atcaaagtcc acgctttgaa gaaattcctt tatttttacc gcagtactta
     2281 aatttcattc gtgaacaagt ggagcaataa ttagctaatc aaatccatga cgaagagtaa
     2341 gaggttgact cataagtcaa aaaataaaga aaaacgcatc aaatccattc taaagaatgt
     2401 tgatttgatg cggttcctat ttttataaac tatcattttc gactaataaa actacttatg
     2461 agtcagcctc tctttttttc ttgattttta tacctctttc taggtaaact aaacatctgc
     2521 caaaaatacc gtgtctcatt gaaacattct caactatttc aaaataaagg ggagtaccaa
     2581 atgaacgagt atattgaaat tatccacgca agagaaacaa cttaaaaatg tcagtctcaa
     2641 aattcccaaa aataaaatta gtatttttac cagcgtatct ggttcaggaa aatcatcgat
     2701 tgtttttgat actattaccc aagaagctgg tcgtcaactg aatgacacct atgatagttt
     2761 tacccgttta tttttaccta aatacagtcg tccagaggtt gatgagattg ataatctttc
     2821 tacggcgatt gtggtcaatc aaaagaagat tggtggcagt gcgcgctcaa cgttagggac
     2881 gattactaac atcaatgcgt tatttcgtgt gttgttttca tgttttgcaa cacctagttt
     2941 aggatatgca aatgcttatt catttaatga tcctagtgga atgtgttctg tctgtcaggg
     3001 aatcggtaag acgattgcat ttttaatagg attgctttat ttttcggcta ttgataatgc
     3061 tgattttgta ttttctgatc catttaccgc agaagttttg tctggaattg aggaacaggc
     3121 aaagaatcat ggttattttg tattggtgca taatgttaat tcagcagaag atgttaaaac
     3181 tattcagaga aattggcgtt ttgaggggtt cattgttgtt ggtgtacgtg taaaagaatt
     3241 tttggaatta aataacgttt ttgatggtcc tgttgtttat attgatacgc atctttcaaa
     3301 agagacatta agagaacttg aacaacaaat gaatcgttta tttgtaaata caaatgatta
     3361 ccaagctagt acattggcag tggaacattt gattcattat ggtcatgaga aaatagcgtt
     3421 tctttctttt gactacattg taggtgaacc aagtgttatt caagaacgac atcaagctta
     3481 tatcgataca ttaaagaaat atcatttaac ctttaaatcg cattggatat ataacaactc
     3541 tgaatttgag cgaatttatc aagaattagc tgaatttact gcaattgttg ttactggaga
     3601 ttatttagcg gctaaattag taaaattttt aaaaaataag aataaacgag tgatggaaca
     3661 attatcgatt attggatttg atgatattac ttttgcagaa ttaatggatc ctgcgttgac
     3721 aacaatacga ctaaatccag gtaacaaggg ggcgattgct tttcaccaac tgattggact
     3781 ggccaaacaa gaagaaataa aaaatcaagt actactatta gagggagaat tgatttctcg
     3841 agactctgtt cacaaaatca aataaaaaag ctctgtgaaa taagtatagt taacaataaa
     3901 atatccgaac gatagagaga atgaactgtg atctagcagt tcctcgtcgc aaattcacta
     3961 gaattagcat tatttactct aaattgttcg gatatttttt cttgttttta attatgatta
     4021 cagtgcctat ttttaacgaa atttgcttat tttcaaaatg tatgattata tgttagtaaa
     4081 cactttcaaa tatgaaaaga ggagctatta tggtaggaat tagggatgtt gcaaaacggg
     4141 cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga ggatccgaca ctttcagtaa
     4201 ccgatgaaac caaacgaaag attgcagaat cggttaattt ttatcattat gtcaaaaaaa
     4261 gtcccgttgc aaaaaattta acaaatattg cattaataac gacagtttct gaagtagatg
     4321 agttggaaga tccctatttt cgaacgattc gcagagggat tcaagtcgaa gcagaacaaa
     4381 gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac accgttggag caagacgatt
     4441 tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct atcttctgtg attgaagaag
     4501 tatcagcaat taattcgaat gtcgtagtga ttgatgaccc tagtattgaa gctgaagtag
     4561 atgcagttta tactgattta acaaaagcaa cgaatcagca tttagatcga ttgtataaaa
     4621 aaggacaccg aaacattgcg tttatcggag gaggacgtgt tcgtttagat agccaaggaa
     4681 ataaacacgt gtcagatgat gattatcgaa aaatagccta tgagacgtgg atgaaacaaa
     4741 aaggattaga aaaatatagt caaatttatt tagaaggctg gaccacactt gacggaatgc
     4801 aggcagcaga tttacttttg aaaaaacatg ctaaagataa gttgccgact gctatcatgg
     4861 taggaaatga tcctattgct gtgggggttt atcgttcact tcaaaaaaat ggtctacata
     4921 ttccagaaga tatttcgatt gtgagttttg ataatattga agttgctgaa tttttaacac
     4981 ctgcattaaa tacggtgaat gtgaatacag aagaaattgg aaaactagca gttcgcatgg
     5041 ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa agtgattgtt tctaataata
     5101 ttattgttag agaaagtgaa aaaaatatcg attaaaaaag taaacatcca aaaaagggtg
     5161 tttacttttt tgtttactaa tatttttagt aaaaaatctc gacaaaacat taagtaaaca
     5221 gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca ggaggaatta agatgaaaca
     5281 tttcaaaaaa atagctttaa cagggatagc tgttttaact gggacattac tcgcagcatg
     5341 tggtggggga gatagtaatt ctaaagacgg gcaagtaact ttaagctatg cgagttgaga
     5401 tactgaccaa gctccaggac taagaaaaat gttagatgaa tttgaaaaac aaaatcctga
     5461 tatcaaagta gaaatggaga caacgccttg ggatcaatat tgggttaaat tagaagctgc
     5521 aacaacggga ggtaatatgc cagatgtagt aaccatgcac tctagcgaat cctataaata
     5581 tatgtcacag aatgctttga tgggacttga tgatgttatc aaagaaaatg atattgacat
     5641 gggaaacttt actgaaggaa ttgctgattt ctatacgctt gaagacgaac tatacgcaat
     5701 ccctaaagat gcttctgtag tggggctatg gtacaacaaa gaattatttg ataatgcagg
     5761 tgtcgattat ccagatgaaa catggacatg ggaaacactc agacaagctg caattgattt
     5821 aacagatgaa tcaaaaggca tctatggttt tgcatcacat attggaacag agactggcta
     5881 ctggccattt atttatcaaa atgggggaga agtatttgcc gatgacaata caaaatcagg
     5941 tttgaatacc ccagaagcac aagaagcttt agctttttat cgagatattg cacttgttga
     6001 tcatgcttca ccaacaatta cagaattaca agatcctgat aagttaagtc gttttcaatc
     6061 aggtagatta gcaatgatga ttgaaggaaa ctggtttgca ccgggatttt tgaaaaatga
     6121 ctatctagtt gaaaatgttg cttctgccgt gttacctaag ggaaaagtta atgcaacaat
     6181 tacaaatgga cttggttggg ctgccagtgc gagtacaaaa tacccagaag aagtgaaaaa
     6241 actaatggca tttttagcta gtgaggacgc caataaaatc caagcggaaa cgggggcatc
     6301 aatcccagca ctaaaaggct ttgaagacga gtgggctgct tcttcagata caattgattt
     6361 gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt ccatacaata aagatggatt
     6421 acgagctgaa caagcagaag ttcaaattat taaccgtatt ctgtctggtg aagtaactgt
     6481 tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg atattgagcg gaaattaaat
     6541 tagttaatta tacaatatac aggcgaaagg attctatttc gtctgtatat taatggatga
     6601 ataccaaaaa gggggatatt atgaaaaatc gaatttttgg aaaatatcgt aaagatcaag
     6661 tgatatgggg ctatattttt atttttccaa cggcattagg attaatttta ttaaatatat
     6721 ggccagcaat tcaaacagca attttaagtt ttcaaaaaac ggtagggttc ggtgatactg
     6781 aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga cggtgaagtt tttcagtctt
     6841 tattaaacac attaatttat gcagtggttt cgatacctag tattgttgta ttatccttgt
     6901 tggtggcagt tttaatgaat agaaaagtta aaggattgtc gatttatcgg acaatttatt
     6961 ttctaccagt cgttgctgca ccatccgcag tcgcaatgat ttggcgctgg atgttcaata
     7021 atgattatgg actgattaat aacatgttgg ctaaaatagg tctcgatgga ccagcatggt
     7081 taacggatcc atcgattgcg atttattcta ttatcattgt aggagtttgg agtgctattg
     7141 gttataacat ggtgttatta ctagccggct tataagaaat tcctaaagat tattatgaag
     7201 cagcggaaat tgatggtgct agtccaatcc agcagttttt caacattacg ttaccattag
     7261 ttacaccaac attatttttt gtagttgtaa caacagttat caatgctttc caagtatttg
     7321 atgttatttt tatgatgatt actcctaata gtaccgccat gtttaaatca caatcattag
     7381 cctatttatt ctataaacat tcatttattt taaatgacaa gggatatggt tcggcaatcg
     7441 ttatgttcct tttagtgatt atattaatta ttactgcaat tcaaatgaag ttgcagaaaa
     7501 agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa taaatttttt tcaaatgcgc
     7561 caatccatat catgcttatt ttaggtggta ttgcaatggc attacctttt atttggatga
     7621 ttttaacttc agggaaaacg ttggttgaat ctacccaaat tccacctaca attttccctg
     7681 agaaatttca gctaagtaac tatcaacaag tacttgattt attaccattc atggcttttt
     7741 atatcaatac atttttaatg gttgttggac gagtgattgg ctcggtttta ttttcagcaa
     7801 tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa aaatatcctt ttcagcttag
     7861 tacttattca aatgatgatt cccggttcca tttttattac tccacagtat ttattagcac
     7921 aaaaattggg tattttaaat acaattccag cattaatatt accaggggta gttagtgcgt
     7981 ttggtacgtt tttattaaga caatttttct taggtttacc tgatgaattg gaggaagcgg
     8041 cctatttaga aggtgccaat atttggacga tctttttcaa aattatgttg ccgcttgcta
     8101 aatcaggatt ggttgcactt ggaatattca cagcgttgtt tgcatttaaa gatttaatgt
     8161 ggccattgat tgtcaatact tcaattgata agatgccttt agctccggga ttagcaaact
     8221 tacaaggaca atatgccacg aacttcccac aattaatggc aggttcgtta ttagcaattt
     8281 ggccaatagt tgtgatattc atcgttttcc aaagaaaatt cattgaagga attgctacaa
     8341 caggtagtaa attataaaac tattaattag tgattggaga gtaagtacat gacaaaaatt
     8401 acatttattg gtgcaggaag tactattttt gcaaaaaacg tattaggcga tgcgatgtta
     8461 actccaagtc tacaagatgc acatattgca ttgtacgata ttgatgaaaa acgactaaaa
     8521 gaatcaaatt taatgcttaa aaatattaat aaaaattcga atgaaaatcg tgctgatatt
     8581 gtttcttatt atgatcgcaa agaagcgtta agagaggctg attttgttat caatgctatt
     8641 caagtgggtg gttataaacc ttcaacagtg attgattttg atattcctaa aaaatatggg
     8701 ttgcgacaaa ctattggtga caccatgggg attggtggga tttttagaac cctacggaca
     8761 ctaccagtaa tgttagactt tgctaaagaa atggaagaag tttgtccaaa tgcttggtta
     8821 ttgaactata caaatccgat ggcgatgtta accatgggtg ttttaaaagc gacgagtatt
     8881 aagactgttg gtttatgtca tagcgttcaa gtatgcgtac cagaattgtt tgagcattta
     8941 ggaatagccg atcaatatga cttaaatgac tttcaatgga aaattgcagg cattaatcat
     9001 atggcattct tgttggaaat tacaaaaaat ggggaagatt tctatccaac aattcgtcag
     9061 ttagctgcag aaaaaccaaa tccacatcca gattctgtac gttttgaatt gttgaaacgt
     9121 tttggttact atattacaga atcaagtgaa cacaatgcag aataccatcc atactttatt
     9181 aaatcaaaat acccagaatt gattgacgaa ttagggattc cgttggatga atacttacgt
     9241 cgttgtgaac aacaaattaa tgattgggaa agcatgcgag aggacattgt aaataatggg
     9301 gagttaacac atacgagaag ccgtgaatat gcttcatata tcatggattc tattacgaca
     9361 ggaactccgt cagtgattgc aggaaatgtc ttaaataaag gcttaatcac aaacttaccg
     9421 acagatgttt gtgtggaagt accttgttta gtcgataaaa atggcgtgca accaacctat
     9481 gtcggtgaat tgccaacaca attagctgct ttaaatcgga cgaatatcaa tgttcaagaa
     9541 ctgacagtag aagccgcttt aacactagat aaatcaaaaa tttatcaagc agcgtattta
     9601 gatccacatc tatcatctga attgtcttta acagatatca aaaatttagt ggatgattta
     9661 atcgaagcac atggggacta tttacctgaa tataattaaa aagaagctga aagagtaagg
     9721 aattttcaat tccttactct tttcttagtt aaatttagta aaaatattga caaaacactt
     9781 agtaaacagt tacattagtg ttaacaaaaa gaaaacgcat tcaaattgta taaggaggag
     9841 cataaaatga agtggtctaa aaaaattatt ttagcttgta tagcactagg agcaacgatc
     9901 tctttgactg cttgtggaaa aaatcaggaa gatgcaagcg gtaaggtaga aatagagttt
     9961 tttaatcaaa aaaaagaaat gactcaaacg attcaggaga ttgctaaaga ttttgaagca
    10021 aaaaaccctg atattcatgt aaaagttgtg gatgtaccga atgctggtga agtaattaaa
    10081 acacgtatgc ttgcaggcga tgtaccagat gtcattaatc tttatcctca aagtatagaa
    10141 ttgaaagaat gggcgaaagc tggttattta gaggacttaa ccgaagaacc gtatttagag
    10201 aatatcaaaa atggatatgc acaacgtttt gctattgagg atcgtgtata tagcatacca
    10261 ttaaccgcta atgtctatgg tttttactat aacaaaactg cttttgagga aatggggatt
    10321 caagcaccag aaacttgggc agaatttgaa aaaatagtag ctgagattaa agatcaaaac
    10381 aaagtaccgt ttgcgattgc aggagcagaa ggctggactt taaatggtta tcaccaacta
    10441 gctatagcga ctgctgctgg tggtgaagag gaagcgaaca atgtttggcg tttctcagaa
    10501 gttaatggaa ttaatgctga aagcaaagaa atgcaaaaag attttaatcg attagatttg
    10561 ttaagagaac caaaagcttt gcaaaacaac tggcaaggtg ccggttataa cgatacagta
    10621 gttactttta caaaaggtga tgcgttgatt atgccgaatg gttcatgggc aatgccgatg
    10681 attcattcac aaaatccaga ttttgaagta ggaacttttc cttttccagc agatgaagca
    10741 ggtcaaagtc taacgattgg cgcaggagac ctagccttat cagtttctgc aacttctcaa
    10801 cataaagaag aagccaaacg ttttgtagaa tatatgacaa cacctgaagc gatgcagaaa
    10861 tattatgatg tagatggtgc accgtgtgct gttgagggcg ttatcgagaa tactcaggat
    10921 tcaccattaa gtggtttaac tgaacttgct tttactgatc gtcatttagt ttggttagcc
    10981 aaagattgga atagtgaaaa tgatttttat accttaacaa caaattattt acacaatggc
    11041 aatcaaaaag ccatgattaa cgcattaaat gctttcttta atccaatgaa agctgacgga
    11101 aggtaggaaa ataaaatgaa aataaaagaa attttaaatc gttactgggc aatcattttt
    11161 gtagcaattc caattatttt gcagatgatt tttttctact ttccaatggt tcaaggtgct
    11221 ttttacagtt taactaattg gactggtctg acttataatt ttgattttgt gggtttgaac
    11281 aattataaga ttcttttttc tgatccgaag tttatcaaat cgattggctt cactttaatt
    11341 gtgacgcttt gcatgattgc tggacaaatt ggttttggta ttttgattgc gcgggcgttg
    11401 aacgcaaata ttaaagggaa aacattcttt agagcatggt tcttttttcc agcggtctta
    11461 tcgggcgtgg ttgtttcttt agtatttaaa caaattttta actatggttt accaaaaatt
    11521 ggtagtatgt taggcattga atttttacaa accagtcttt taggaacaac atggggagcg
    11581 atttttgcag taatttttgt tttactttgg caaggaattg ccatgccagt catcattttc
    11641 ttagcgggtt ttcaaagtat tccacaagaa attattgaag ctgctagaat ggatggggcg
    11701 aatgataagc aaattttttg gaatattgaa atcacctact tattaccaag tatttcaatg
    11761 gtatttatta tggcattaaa aggtggctta acagcatttg atcaaatttt tgctttaact
    11821 ggtggtggac caagcgatgc cacaacatct ttggggttac ttgtttataa ttatgctttt
    11881 aaaaacaatc agtttggtta tgcgaatgct attgctttag tcttatttat cttaattggg
    11941 attgtttcac tggcacaatt aaaattatcg aaaaaatttg aaatctagga ggtttcccaa
    12001 tgaaaacatc aaaagaaaaa aatatttcta aatacttgct tttattatta gggggcttca
    12061 tgattttagt tcccttagtt tcaacgctat tcggttcatt taaaacaaca cccgatatta
    12121 tgaataattt ctttcgtttt cctaatccag cgactttgga aaatttccga agattgttag
    12181 cggatggaat tggtcattac ttttggaatt cggcaatcat cacaatcatt gcagtagttg
    12241 ctgtgacagc aattattcct atggctgctt attcactagc aagaaaaatg actcgtggga
    12301 aagcatacgg attgatgtat ttatttttaa ttttaggaat ttttgttcct ttccaagtaa
    12361 ttatgattcc gattaccgtt atgatgagta aaattggttt agcaaatatg tggggattaa
    12421 tcttactcta tctcacttat gccgtaccac agacactatt tttatatgtt ggttttatta
    12481 aactaaatgt gcctatcagt ctggatgagg ctgctgaaat tgatggggct agtcacttta
    12541 caacttattt caaaattgtt ttcccaatgt taaaaccaat gcatgcgacg actattattc
    12601 ttaatgctct atggttttgg aatgatttca tgctaccatt attaatttta aatcgtgatt
    12661 ctaatatgtg gacactacca ttgtttcaat ataattatac gggacaatat tttaacgact
    12721 atggtccaag ttttgcttcc tatacagtag gaattgtcac gattacacta gtttatcttg
    12781 ctttccaaag aagtattata gctggaatga gcaatggagc agtgaaataa ccaataaatt
    12841 attctcatta taaaggagtt cataaactta tgattatatt tgacaatcac acacaaacat
    12901 ttcatttaca aaataaaaaa attagctatc tattaggaat cgaagaaaat aattatttaa
    12961 cgcatcttta ttttggaaaa aagattacgc actattctgg tgggtatcgc tacccccgaa
    13021 cagatcgttc cttctcaccc aacaatgcaa atgcgcaaga tcgtttatta tctttagaca
    13081 cattaccatt agaatttcca ggatatggac atggtgattt tagagaaccg gcgcataata
    13141 ttaaattagg aaacggtagt cgtgtaaatg attttcgtta tgatagctac gaaattcaaa
    13201 aaggaaagcc cagcttagcg ggattgccag ccttatatac ggaagatgat gatgaagctg
    13261 aaacgctgat tattcaattg aaagacgcga ttagtggatt gactttacag ttgttctaca
    13321 caatttatca aaatcacagt gtaattattc gttctacgca attagtaaat aatagtgatg
    13381 aaacagtgga gattaaccgc ttagctagtc aatcaattga ttttccaaat cgtccactcg
    13441 agttaattca tttaaatggt acctgggcaa gagaacgtca aatgactaga gaaaaaattc
    13501 atacagggat aaaagtatta gatagtaaac gcggatcaag cagccatcaa caaaatccgt
    13561 ttgttgcttt agtagatgcc aaaacagatg aatttcaagg agaggcatat ggatttaatt
    13621 tagtttatag cggtaatcac gaaacggtga ttcaaaaaga tccatttgac caaacacggg
    13681 tcattatggg aattaattca tttaattttg gttggcaact aaaaactggc gactcatttc
    13741 aaacgccaga agtcgtgatg gtttatagta atgaaggtct gaatacgtta tcacagacgt
    13801 atcatgaatt gtataatcac cgtttaattc gtggaaacta tcgtttaaaa gaacgtccca
    13861 cgttaatcaa caactgggaa gcaacgtatt ttgattttaa cgaagaaaaa atcatgaata
    13921 ttgtcgatga tgcgaagaac ttgggcattg aaatgtttgt tttagatgat ggttggtttg
    13981 gcggtcgtga aaatgacttc acttcattag gtgattgggt tgaaacagaa ggcaaacttg
    14041 cttgtggttt ggaaaattta gccaagcaag tgcatgataa cgggatgaaa tttgggattt
    14101 ggtttgaacc ggaaatgatt tctgaagata gtgaactatt tcgagcacat ccagattggg
    14161 cattgggtat tccaggtcgt gaccgctcaa ttagtcgaaa tcaatatgtt cttgattttt
    14221 caagacaaga tgttcgtgat aatatctatc aacaaatgac ggcgattttt gatcgtgtac
    14281 cgattgatta tgtgaagtgg gatatgaatc gaaatatgac agaagtctat tctgtcttgt
    14341 tggactcaac gatgcaaggt gaagtggctc atcgttatat gttagggtta tatgaatttt
    14401 tagaaaagtt aacaacgaat tatccaacta ttttatttga aagttgttcg ggtggaggcg
    14461 gtcgttttga tgcaggtttc ttgtattaca tgccacaaac ttggacgagc gacaataccg
    14521 atgccgtggc tcgtctgaaa attcaatatg gaaccagctt agtgtatcca atttcaagta
    14581 tgggtgcgca tgtttcagca gtcccaaatc atcaaacagg aagagaaacg agtttaacta
    14641 ttcgtggcaa tgctgcgatg gctggtgttt ttggttatga gctagatcta acgcaattaa
    14701 cttctgaaga aaaagcagaa attaaagaac aagttgcctt ttataaaaag catcgccaac
    14761 ttttgcaata tggaactttt tatcgcttgg aaagtccgtt tgaaggcaat gatactgcat
    14821 ggatgtttgt ctcaaaagac caaacagaag ccatggtctt ttatttccga gtattagcag
    14881 aagctgcgca tccattagtc acattgaaat tagcaggatt agcagaagaa gcaacttatc
    14941 aaatggaaga tgtaacattt gctggcgatg aattgatgaa tctcggtttt tatattgact
    15001 cagaactaca tggtgattat gcgacacaac gcttttattt gaaacagata tagtagcttg
    15061 aatttacaat ataagtgcaa caccaaaaga gactcattcc aaacgaagaa aatatgtttt
    15121 atttacacgc atttactaaa aagcagcctg atttgaattg ggaaaatgaa gaagtacgtg
    15181 aagaaatcta tgcaatgatt aactattggt tagataaagg actaggcgga tttagaatcg
    15241 atgcgatatt aaacatcaaa aaaaaactag agtatggcat ctttgaatct gatggagaag
    15301 atgggttagc cttcattggg ccctggattc ttaatcagtc gggcattgaa gtgtggttaa
    15361 aagaaatgag agagcggaca tttaaaccac ataatagtat gacggtagca gaagccgatg
    15421 ttcctaatga acgtttagat gaatatattg gtgaagatgg ctattattcg atggtttttg
    15481 attttagtta tacggatatt gatgtacctg aaactggtga atggtttaaa gatagccaat
    15541 ggacatggac ggatatgcgt accaatattt ttacgaatca attagttacg caagataaag
    15601 gttggggcgc cttatattta gaaaaccacg atcaaccacg ttcaattaat aaatatattc
    15661 cagaagaatg gatcaatgat tatagtaaaa aaatgttggc gacgcttttt atgttactaa
    15721 gaggtacccc ttttatttat caaggacaag aactaggaat gaccaacata gaaatggcat
    15781 cattagaaga ttttgatgat gtagctacgc attctcagta taaacgggca ttagaatatg
    15841 gattggcacc agagcaagca ttgaaagctg tcaataaacg aagtcgggat aattctcgca
    15901 cacctatgca atggaatacg caaaaaaatg ccagattctc aacttctgat aatgtatggt
    15961 taaaagtaaa tcctaattat ccagaactca atgcagaagc tcagttaacc aatcaacagt
    16021 cggtattcaa ttattatcat caattaatta aattaagaaa aaatagtgta tacaaagacg
    16081 tgctaatcta cggacaattt ctaccagtga aagataaaaa tgaacatatt ttgctttatg
    16141 aacgccaatt agaagataaa atcgtattag ttatcttaaa cgttacagca acagaacaag
    16201 aatattatgt agatgaacag taccaacaag tattaatcaa taattatgaa gatgttttat
    16261 tagatcaaag tcgaaaattg cgtttacgac cgtttgaaag tattgtatta gctaattatg
    16321 gaggacaatt atgaaaatta aaaacgaagc aatgctcatc acctatgcag atagtttagg
    16381 aaaaaacatg ggcgaattaa aacaagtctt agacaaacat ttacaaggtg ttattggtgg
    16441 tgtacatctg ttgccttttt ttccatcgac aggtgatcga ggctttgcgc cgagtgatta
    16501 tacgacagtt gatccagctc ttggaacatg ggaagatgtg gaagcattag gtgaagatta
    16561 ttatttgatg tttgatttta tgattaatca catttctcgt gaatcaaaat ttttccaaga
    16621 tttcaaaaaa aaccatgagc agtcaccata caaagaaatg tttatccgta ttcatgaatt
    16681 tttcccagaa aatcgaccaa cacaagaaga tattgattta atctacaagc gtaaagacaa
    16741 agcgccgttt caaacggtag aatttgcgga tggtacgaca gaagaggttt ggaatacctt
    16801 tggggaagaa caaattgatt tagatgtgac caaagaagtt gtgaaagaat ttattcgaga
    16861 aaccatcaaa gatatggcga accatggctg ttctttaatt cgattagatg cgtttgctta
    16921 tgcgattaaa aaattagata ccaatgattt cttcgtggaa ccagatattt gggaactttt
    16981 agacgaagtg cgtcaagaag cagcgaaata tgacgtcgaa ttattaccag aaatccatga
    17041 gcattattcc attcaaatga aaattgccaa tcatgattat tatatctatg acttcgcctt
    17101 gccaatggtc actctgtatt cactttatag tggaaaatca gaacgtttag ccaattggtt
    17161 gaaaatgagt ccgatgaagc agtttacaac attggatacg catgatggaa ttggtgttgt
    17221 ggacgctcgt gatttattaa cagatgaaga attggattac acctctgagg aattgtataa
    17281 agtcggggcg aatgtgaaga aggtctattc aagtgccaat tacaataatt tagatattta
    17341 tcaaattaac agcacctatt atagtgcatt aggcgataat gaccgtagct atctgttagc
    17401 acgtgcgatt caagtatttg cgccagggat tccacaaatt tattacgttg ggttattagc
    17461 aggaaaaaat gatattgaac tgttggaagc aacgaaagaa gggcgtaata ttaatcgtca
    17521 ttactatgat ttagccgaaa ttgaagaaga agtgcaacgg ccagtgattc aacaattatt
    17581 tgcactttta aaatttagaa atgaatctgc ggcgtttgat ttggatggaa cgattgaagt
    17641 agcaacgcca tcagaatcag aaatagtgat tacacgtaaa aataaaaacg gtagtgatag
    17701 tgcaacatta acggctaact tggatacgaa agaatttatc attgagtcta aagatataaa
    17761 aattttcact agtgttgcat taaaatttta acctattcca tagaacaaaa aatcctttat
    17821 actagcactt ggatgactta tccaagacca ataaaaagga ctcatacatg gataagtata
    17881 aaacaaatac agcatttaat aaatggtttt ctgccataaa attaggaaaa ctttctgtca
    17941 actcccaaaa atgtattttt gattacaatt cgtatcgtaa aaaactgtct tttgaagctg
    18001 tgctcaagct ctttctctac gcagttaatg aagaaaaaga aagtttgcgt gatttgagta
    18061 cagctcttat cgaccgatcg ctccaactag aaaccgatgt gtcatcgatt agtcataccc
    18121 aattgtctcg agcatttaat gcacttgaac ctaaagtgtt agaagaaatt tttcaacaac
    18181 tgttagaaaa agttcaacat caaatcaaac caaccaagcg aaatcatctg tatctgattg
    18241 attccagtac gttttcgctt agtttaaaac gtcataagtg ggcgactttc cgtcaaacaa
    18301 aatcaggtgt caagttacac ttaaactatt gttatatgga cgatactacg atgtatccaa
    18361 ctgattttac cttaacaaat gcccatgaac atgatctcaa tcaactacca gtattggtga
    18421 atcaaccaga ggcaacttac gtgtttgatc gtgggtatta ggactttgaa aagatggatc
    18481 agatgcattg ggatggctat ttcttcgtca ctcggattaa aaaaaacacc aaagttcacg
    18541 tgatagatac tctggaaaca tcgccagaaa cagaaattct acgagacgag ctggttcgtt
    18601 taggatctaa aacctatcta accgccaatt tccgactagt cactgttcaa gataagaacg
    18661 gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc ttcaaaagaa atctctgata
    18721 tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca catcaaacaa cacatgacca
    18781 ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa tcaattgatt ttaacgatga
    18841 tttccgctct cctcacgttt ctcatcaaat tggagacgaa aacggaaaaa tcagtgttcc
    18901 aaatcaaacg attctttcgt tatttactgt ttcagccctt tgaatgttgt ctagaaaaat
    18961 taattcccac atgagaaaaa agaaatttgg ctcgttactt ttggttttca gttgaataac
    19021 agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag cacagttttt tttgacttat
    19081 tttcagagga aatagttttc agaatattct aaaatgtttt atgcaacact agtgatattt
    19141 tttagaatta gatttctaat gtgcattttt tgagtattaa ggagcaagta tacaaagatt
    19201 tataaagctg attaaatttg aataaaaata tgcaggaacg ctactatttt tagtagtgtt
    19261 cttttttata tttgaataaa aaatgtggtg gttaaatggt ggttataaca aaaaatgaat
    19321 taatgaaatt aggttatggg aagtatcagt ctgagagaat catacgacag gcaaaagcaa
    19381 ttatggtttc aaaaagctat acctattacg aaaatagaag attaggacta gtacctgttg
    19441 aatctgtaga agaagtttta ggtatgaaga tagaggtaga ggaggatttt cagaatcatg
    19501 gttaatttta aaaaagataa aaatgggaaa tatcatttta ctgctagttt gggattcgac
    19561 aaacgaacag ggaaaagagt acaaaaaaac gtagaggggt taaaacagtt aaagaagaaa
    19621 aaaagcgtat accgaacttg tttctaatta tggaaaagaa gcagaaactt ctcaaagtac
    19681 tatgttgttt gaggagtttt tttataaatt attcctacct tattatcaag ggaaagtaga
    19741 agaaagaaca tataaaaatc ggttatctac aatcaaaaaa acttttctta tttctttaaa
    19801 atggagttaa gagaaatacg tccaattgac attcaaaagt ggcaaaataa tttattgagc
    19861 aaatataaaa attcttatgt aagaaacatg tatgggattt ttcagatgtc attagatcga
    19921 gcagttgttt tgggactact tgaaaaaaat cctgcaaaag ttattggaaa tgtgaagaaa
    19981 aggaaaacta cagttgattt ttggacaaaa gaagaattta caaaagttat ttcaactttt
    20041 tatattgatg atttgtacca acattttggt tttatttgta tgtggttact ttttatgacc
    20101 gttatgcgtt tgggagaagc cactgcatta acttggaaag atattgattt tgaaaataaa
    20161 aacttgcgtg tgaataaaac actctattat aagagagcca aaacctatga gtttgtagaa
    20221 cccgaaacgg cttctagtat tcgtacaatc gaactagacg acaatacaat taccttttta
    20281 aaagcttgga aggagcgtca agtttcaata ggaggcatgt cgtttgtact ttcttataat
    20341 ggaacaccta ctcagaaata tacaatccgt catattatga gaagacatgc aacgatcgtt
    20401 ggtgttcata taatccgcat acacggatta aggcatagtc atgcttcttt gcttatatca
    20461 caataaaaag atgcacttcg aattaaagaa aggttaaaac atgctgatgt acaaacgact
    20521 ttaggaactt atggtcattt gtatctaaat tcattgagag agataacaaa tgatcccaca
    20581 gattatatcc aaactaattt ttctaaaatc aaaccgacac ctatttacga taataaaaaa
    20641 taaatgataa acaaaagtag gatcacacca aaagaaacac actagaaaaa ctataataca
    20701 ggcttttcta gcgtgttgtt tcttatttcc gcctatttat acattatact cataaagcat
    20761 ttaatgcatc gatcgtagcc gcattgtcta tatgggtata taaagttgtt gtattggtac
    20821 ttgaatgtcc caattgttgg gcagttaata ccaatgaatt tgtctgttgg tagagtctag
    20881 tagctaatgt atgacgcagt ttgtgagggc tgacttgaat tctaaagcct tttgaatatt
    20941 ttgctaccat ccgttcgatt gcaataccgg atatacgctt ggcttctcct tggtaaattg
    21001 ctagaaataa agctttttcg ttttctgtta ctttatatcg ttctttccga atagctagat
    21061 aattagctaa atattccatt gcgaatgctg ctatattgac agcatctctt ttatttcctt
    21121 ttctagttac taccacatta ttcttatcta aagataggtc atctaaatta atatttataa
    21181 cctcggagag tctcaatcca gatcctaaaa tcagtgcatt gatcgccagg tctctttcta
    21241 catctcgttg ataatagatc aaagctcgtt tactcaaagt atttttatat tcattggcaa
    21301 tataatctag ataattttgt gtatccttat ctagaaataa ttttttctca atggcattgg
    21361 cacgtgcagc taacgtagtt ttatctgtga tggttgctac ttgatccata ggattattgt
    21421 ctaagtatgg ttttcctaga ggattattgg aacttttcga aagatattta aatagaacct
    21481 ttaatgccgt gatatttcta ttgatagtag aagtagatag acctaagtta ttattatctt
    21541 tgagcttagt ttttgttgtc tcttttttta cattttcttt tgtgcgacgt ttcaaatgag
    21601 atttgtacaa gtttaggtca taggcagtta actgtcttat cgaggtgata gaagtatcag
    21661 gaacgataga ctcactcaga agccattcat aaaagagctt ataatttttt agatattcta
    21721 aagtggttcg aggtgattga tctttatcct cacgatcttg aacaaaatct tttatataat
    21781 cgggcatatg aagtatgatt gtatctattt tttgatatat ttctgtattt ttcatgtcat
    21841 tcatcctttc aataagtagg ggagaagagt atctctataa ttggactata ccaattatag
    21901 aatgttttgt caattggtat gctctaaaat ttattaattt gtttcaaaaa aagcatataa
    21961 acaggtatac gtttaataat ttaaaataaa tggtatacat aaatagggaa ggaaatagaa
    22021 gggagcaatc taaatttttt gaattttctt aattaattat atagaaagaa caatctttct
    22081 atagagttag acatgagagg gactatgctt gatttaaaat caacaagcgt atatactgta
    22141 tatacaaaga aaaaggaggg agaagactat ggcaattaaa gaaagaaaat ttagggaagt
    22201 ggggaactca gtagtattaa ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc
    22261 cggagatacg gtttttttag atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc
    22321 cttatctgat gaaattgatt tagcaatatc tcaagcatta actgaatatg atactacttt
    22381 tagagagctt gtcgataaat gagtattcat tacctagaag ccaaagatat tataaaaatg
    22441 aatgtaatcc aaataaaaaa atactctcct aatgaacccc ttggtgtaaa ggatgcgaat
    22501 gctttagaaa tgtgtgtagg gcaacttcga gcctcagctt ttggtgaaga ggtttatcct
    22561 tcgatttatg aaaaagcagc tatcctactt atacaattga taaagaaaca tccttttcac
    22621 aatgcaaata aacgtactgc tttcttagct acttttgtct ttttgaaaat caatggacag
    22681 cttttagcta ttgatcaaaa agatgctgta aatttagcgg tatatattgc tacgtatgat
    22741 aaggatttcg atcaactaaa atatgaagtg atcaacccta taaagacaca tacgcagccc
    22801 atttaaggag ttactgtatg tgtctttata gggttattag ctgctatagc gaaataattg
    22861 aagtaaatta caaatagcta taattcaaat ccaactatat agaaaacgta aattttctat
    22921 atagttggat taagataacg actatttatt ttatcatatt tttactatac cctatacttt
    22981 tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt tgtaagttag
    23041 attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt
    23101 gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag tttgttgacg
    23161 attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa acttatgaaa
    23221 tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc aataaaaact
    23281 aatgttcccc tcattctttg aacttctact atttgattta tttattctga taaatgacga
    23341 tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag
    23401 taataggata ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct
    23461 gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata aaccataagc
    23521 gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc
    23581 ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt
    23641 actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct
    23701 aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt
    23761 atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg
    23821 gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta
    23881 tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga
    23941 atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca
    24001 actgtattta catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct
    24061 gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg
    24121 ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca
    24181 taggtatttg taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa
    24241 ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat taaattcgtc
    24301 caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta
    24361 tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt
    24421 atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt
    24481 cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca
    24541 atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc
    24601 aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt
    24661 cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg
    24721 ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt
    24781 agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca
    24841 aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat
    24901 attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac
    24961 aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac
    25021 gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa
    25081 ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata
    25141 ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat
    25201 ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat
    25261 caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta
    25321 tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat
    25381 tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac
    25441 tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta
//
LOCUS       DAZBTH010000030        25361 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000030 DAZBTH010000000
VERSION     DAZBTH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.030"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHGNW_001205"
     tRNA            12..84
                     /locus_tag="ACHGNW_001205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHGNW_001206"
     tRNA            88..160
                     /locus_tag="ACHGNW_001206"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHGNW_001207"
     tRNA            168..249
                     /locus_tag="ACHGNW_001207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHGNW_001208"
     tRNA            267..339
                     /locus_tag="ACHGNW_001208"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHGNW_001209"
     tRNA            351..422
                     /locus_tag="ACHGNW_001209"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHGNW_001210"
     tRNA            439..524
                     /locus_tag="ACHGNW_001210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHGNW_001211"
     tRNA            535..608
                     /locus_tag="ACHGNW_001211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHGNW_001212"
     tRNA            618..691
                     /locus_tag="ACHGNW_001212"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHGNW_001213"
     tRNA            711..783
                     /locus_tag="ACHGNW_001213"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHGNW_001214"
     tRNA            811..884
                     /locus_tag="ACHGNW_001214"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHGNW_001215"
     tRNA            898..971
                     /locus_tag="ACHGNW_001215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHGNW_001216"
     tRNA            995..1084
                     /locus_tag="ACHGNW_001216"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHGNW_001217"
     tRNA            1097..1170
                     /locus_tag="ACHGNW_001217"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHGNW_001218"
     tRNA            1189..1264
                     /locus_tag="ACHGNW_001218"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHGNW_001219"
     tRNA            1266..1336
                     /locus_tag="ACHGNW_001219"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHGNW_001220"
     tRNA            1350..1423
                     /locus_tag="ACHGNW_001220"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHGNW_001221"
     tRNA            1448..1535
                     /locus_tag="ACHGNW_001221"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHGNW_001222"
     tRNA            1557..1628
                     /locus_tag="ACHGNW_001222"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHGNW_001223"
     tRNA            1651..1740
                     /locus_tag="ACHGNW_001223"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHGNW_001224"
     tRNA            1753..1826
                     /locus_tag="ACHGNW_001224"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHGNW_001225"
     tRNA            1845..1917
                     /locus_tag="ACHGNW_001225"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHGNW_001226"
     tRNA            1921..1993
                     /locus_tag="ACHGNW_001226"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHGNW_001227"
     tRNA            1998..2068
                     /locus_tag="ACHGNW_001227"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHGNW_001228"
     tRNA            2081..2154
                     /locus_tag="ACHGNW_001228"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHGNW_001229"
     tRNA            2166..2237
                     /locus_tag="ACHGNW_001229"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHGNW_001230"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHGNW_001230"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN6925528.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHGNW_001231"
     CDS             3826..5298
                     /locus_tag="ACHGNW_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6925529.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHGNW_001232"
     CDS             5381..6169
                     /locus_tag="ACHGNW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6925530.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHGNW_001233"
     CDS             complement(6236..6904)
                     /locus_tag="ACHGNW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN6925531.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHGNW_001234"
     CDS             7019..8731
                     /locus_tag="ACHGNW_001234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925532.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACHGNW_001235"
     CDS             8734..10503
                     /locus_tag="ACHGNW_001235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925533.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACHGNW_001236"
     CDS             complement(10708..11274)
                     /locus_tag="ACHGNW_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6925534.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACHGNW_001237"
     CDS             complement(11274..12230)
                     /locus_tag="ACHGNW_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN6925535.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACHGNW_001238"
     CDS             complement(12230..12886)
                     /locus_tag="ACHGNW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN6925536.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACHGNW_001239"
     CDS             13256..15274
                     /locus_tag="ACHGNW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6925537.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACHGNW_001240"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACHGNW_001240"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN6925538.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ACHGNW_001241"
     CDS             18463..20016
                     /locus_tag="ACHGNW_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN6925539.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ACHGNW_001242"
     CDS             complement(20397..22181)
                     /locus_tag="ACHGNW_001242"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925540.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ACHGNW_001243"
     CDS             complement(22196..23917)
                     /locus_tag="ACHGNW_001243"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925541.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ACHGNW_001244"
     CDS             24017..24778
                     /locus_tag="ACHGNW_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6925542.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7847 a         4654 c         5381 g         7479 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca aaggttattg ttatttttat atattctttt t
//
LOCUS       DAZBTH010000031        24512 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000031 DAZBTH010000000
VERSION     DAZBTH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.031"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..977)
                     /locus_tag="ACHGNW_001245"
     CDS             complement(129..977)
                     /locus_tag="ACHGNW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6925543.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="ACHGNW_001246"
     CDS             complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="ACHGNW_001246"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN6925544.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3478..3657)
                     /locus_tag="ACHGNW_001247"
     CDS             complement(3478..3657)
                     /locus_tag="ACHGNW_001247"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN6925545.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3704..5527)
                     /locus_tag="ACHGNW_001248"
     CDS             complement(3704..5527)
                     /locus_tag="ACHGNW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6925546.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5805..8084
                     /gene="helD"
                     /locus_tag="ACHGNW_001249"
     CDS             5805..8084
                     /gene="helD"
                     /locus_tag="ACHGNW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN6925547.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8177..8824)
                     /locus_tag="ACHGNW_001250"
     CDS             complement(8177..8824)
                     /locus_tag="ACHGNW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925548.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9003..9722)
                     /locus_tag="ACHGNW_001251"
     CDS             complement(9003..9722)
                     /locus_tag="ACHGNW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6925549.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9853..11004)
                     /locus_tag="ACHGNW_001252"
     CDS             complement(9853..11004)
                     /locus_tag="ACHGNW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN6925550.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11221..11961)
                     /locus_tag="ACHGNW_001253"
     CDS             complement(11221..11961)
                     /locus_tag="ACHGNW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6925551.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="ACHGNW_001254"
     CDS             complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="ACHGNW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN6925552.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="ACHGNW_001255"
     CDS             complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="ACHGNW_001255"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN6925553.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12922..13572)
                     /locus_tag="ACHGNW_001256"
     CDS             complement(12922..13572)
                     /locus_tag="ACHGNW_001256"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN6925554.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="ACHGNW_001257"
     CDS             complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="ACHGNW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN6925555.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="ACHGNW_001258"
     CDS             complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="ACHGNW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN6925556.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15015..15542)
                     /locus_tag="ACHGNW_001259"
     CDS             complement(15015..15542)
                     /locus_tag="ACHGNW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN6925557.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15722..16507)
                     /locus_tag="ACHGNW_001260"
     CDS             complement(15722..16507)
                     /locus_tag="ACHGNW_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN6925558.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16500..17369)
                     /gene="accD"
                     /locus_tag="ACHGNW_001261"
     CDS             complement(16500..17369)
                     /gene="accD"
                     /locus_tag="ACHGNW_001261"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN6925559.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17370..18749)
                     /locus_tag="ACHGNW_001262"
     CDS             complement(17370..18749)
                     /locus_tag="ACHGNW_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN6925560.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="ACHGNW_001263"
     CDS             complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="ACHGNW_001263"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6925561.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19201..19677)
                     /gene="accB"
                     /locus_tag="ACHGNW_001264"
     CDS             complement(19201..19677)
                     /gene="accB"
                     /locus_tag="ACHGNW_001264"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN6925562.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="ACHGNW_001265"
     CDS             complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="ACHGNW_001265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN6925563.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="ACHGNW_001266"
     CDS             complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="ACHGNW_001266"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN6925564.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="ACHGNW_001267"
     CDS             complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="ACHGNW_001267"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6925565.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22707..22931)
                     /locus_tag="ACHGNW_001268"
     CDS             complement(22707..22931)
                     /locus_tag="ACHGNW_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6925566.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22984..23946)
                     /locus_tag="ACHGNW_001269"
     CDS             complement(22984..23946)
                     /locus_tag="ACHGNW_001269"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN6925567.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23956..24396)
                     /locus_tag="ACHGNW_001270"
     CDS             complement(23956..24396)
                     /locus_tag="ACHGNW_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6925568.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6714 a         5443 c         4262 g         8093 t
ORIGIN      
        1 cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa acactttgat
       61 ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata taaaaaatag
      121 taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact
      181 ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca
      241 aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat
      301 gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca
      361 atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta
      421 cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc
      481 aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga
      541 atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat
      601 tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt
      661 atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct
      721 tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg
      781 aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc
      841 aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg
      901 ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat actttcaata
      961 cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca
     1021 tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac
     1081 agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata
     1141 cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta
     1201 ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa
     1261 atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa
     1321 ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt
     1381 atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg
     1441 ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc
     1501 ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac
     1561 aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc
     1621 cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat
     1681 agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc
     1741 gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc
     1801 tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt
     1861 gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac
     1921 tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa
     1981 tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga
     2041 tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga
     2101 atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag
     2161 catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg
     2221 tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc
     2281 tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt
     2341 tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc
     2401 ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt
     2461 caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga
     2521 gtttccacgc caataagcac ctgccactga taataattta aatacttgta tacggccagt
     2581 ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat
     2641 cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat
     2701 ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat
     2761 tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt
     2821 atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg
     2881 atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag
     2941 tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt
     3001 caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga
     3061 aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc
     3121 tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca
     3181 tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt
     3241 tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac
     3301 cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt
     3361 ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa
     3421 tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta
     3481 ttttttccat tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg
     3541 gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat
     3601 attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat
     3661 gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct
     3721 tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag
     3781 agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac
     3841 ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta
     3901 ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca
     3961 agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac
     4021 cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag
     4081 gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg
     4141 catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc
     4201 cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg
     4261 cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta
     4321 catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa
     4381 tctttacttc atgattattg actactgctt ggattccttg accggtcact tctttcagtt
     4441 gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat
     4501 ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat
     4561 tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa
     4621 aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc
     4681 cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg
     4741 gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc
     4801 aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta
     4861 aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat
     4921 actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag
     4981 accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag
     5041 aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc
     5101 caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt
     5161 ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt
     5221 tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata
     5281 tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta
     5341 atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga
     5401 aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa
     5461 caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat
     5521 gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc
     5581 tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat
     5641 tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt
     5701 cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc
     5761 tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac
     5821 tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg
     5881 atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc
     5941 acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac
     6001 atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa
     6061 cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg
     6121 aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg
     6181 ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat
     6241 tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa
     6301 tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt
     6361 tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac
     6421 aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa
     6481 tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata
     6541 atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg
     6601 attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca
     6661 aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa
     6721 gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag
     6781 tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata
     6841 tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa
     6901 caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa
     6961 aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag
     7021 aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc
     7081 gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc
     7141 gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa
     7201 ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt
     7261 tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat
     7321 tctttttact gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct
     7381 ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc
     7441 caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga
     7501 tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga
     7561 caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac
     7621 atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag
     7681 atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt
     7741 attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac
     7801 tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc
     7861 gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct
     7921 tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca
     7981 tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt
     8041 taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc
     8101 tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc
     8161 aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt
     8221 tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata
     8281 ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa
     8341 tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg
     8401 atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct
     8461 catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa
     8521 agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa
     8581 aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac
     8641 actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa
     8701 cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact
     8761 cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt
     8821 ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct
     8881 ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat
     8941 ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt
     9001 gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag atcttccagt
     9061 gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga
     9121 gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct
     9181 tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt
     9241 acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct
     9301 cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc
     9361 atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg
     9421 ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat
     9481 tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt
     9541 tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc
     9601 ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc
     9661 cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc
     9721 atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt
     9781 atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt
     9841 ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc
     9901 tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt
     9961 tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc
    10021 ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt
    10081 gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt
    10141 ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg
    10201 cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa
    10261 caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag
    10321 atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg
    10381 gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc
    10441 acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc
    10501 ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag
    10561 actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg
    10621 aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc
    10681 aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat
    10741 ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc
    10801 cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat
    10861 agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc
    10921 tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc
    10981 tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa
    11041 tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct
    11101 cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc
    11161 ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg
    11221 ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt
    11281 aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact
    11341 ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc
    11401 ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg
    11461 ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa
    11521 aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa
    11581 cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag
    11641 cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt
    11701 gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa
    11761 catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc
    11821 taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg
    11881 tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa
    11941 agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc
    12001 atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac
    12061 gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt
    12121 tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg
    12181 acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac
    12241 gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa
    12301 ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc
    12361 cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg
    12421 gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct
    12481 cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg
    12541 attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga
    12601 atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc
    12661 cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt
    12721 ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct
    12781 ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc
    12841 acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta
    12901 gaataagcag tatattcatt actatagtcc atattcatac aacccttcat tttgaatata
    12961 atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg
    13021 tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt
    13081 atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc
    13141 gattttatac cattttggca aatattctac catatcccca ccgataataa agtaataatc
    13201 tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc
    13261 acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa
    13321 catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata
    13381 agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac
    13441 taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt
    13501 ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg
    13561 cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt
    13621 ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca
    13681 atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag
    13741 taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc
    13801 tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg
    13861 taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc
    13921 tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt
    13981 tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc
    14041 aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc
    14101 acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac
    14161 atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc
    14221 aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc
    14281 ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg
    14341 atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt
    14401 atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt
    14461 tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac
    14521 atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt
    14581 cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt
    14641 catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc
    14701 aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt
    14761 gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta attcattcag
    14821 taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa
    14881 tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg
    14941 cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca
    15001 ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat
    15061 agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt
    15121 gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca
    15181 gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt
    15241 gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat
    15301 ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt
    15361 cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc
    15421 catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat
    15481 tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac
    15541 atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat
    15601 tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat
    15661 ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca
    15721 actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa
    15781 caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg
    15841 gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta
    15901 gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc
    15961 acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc
    16021 agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga
    16081 tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc
    16141 cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa
    16201 tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt
    16261 caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa
    16321 taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt
    16381 catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa
    16441 aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct
    16501 tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt
    16561 ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc
    16621 ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca
    16681 aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca
    16741 gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta
    16801 tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc
    16861 atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt
    16921 tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta
    16981 ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact
    17041 gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat
    17101 gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata
    17161 atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact
    17221 ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac
    17281 atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga
    17341 atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc
    17401 caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca
    17461 ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat
    17521 tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt
    17581 atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg
    17641 tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa
    17701 ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg
    17761 aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt
    17821 tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta
    17881 ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca
    17941 ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt
    18001 gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa
    18061 gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact
    18121 tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc
    18181 gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact
    18241 ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt
    18301 ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg
    18361 actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg
    18421 atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat
    18481 cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt
    18541 gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg
    18601 cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc
    18661 gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg
    18721 cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc
    18781 cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc
    18841 tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg
    18901 gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga
    18961 aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac
    19021 aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc
    19081 aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg
    19141 atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc
    19201 ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac
    19261 aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc
    19321 ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg
    19381 tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc
    19441 atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg
    19501 tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc
    19561 tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc
    19621 cgttagcgag gattgatcaa attgactgac taattctctg atttcattga tatccaaaat
    19681 tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta
    19741 ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc
    19801 tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc
    19861 aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc
    19921 gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct
    19981 tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc
    20041 ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct
    20101 ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca
    20161 tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct
    20221 ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga
    20281 tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc
    20341 gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat
    20401 gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct
    20461 tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg
    20521 aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt
    20581 gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt
    20641 tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa
    20701 aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg
    20761 acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg
    20821 ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa
    20881 gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg
    20941 tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct
    21001 aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc
    21061 ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat
    21121 cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt
    21181 gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc
    21241 aacccagata cactagagag attaatgatg gccccttctc gctgcttcat catttttttc
    21301 aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa
    21361 tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac
    21421 acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa
    21481 tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc
    21541 ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa
    21601 gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct
    21661 tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat
    21721 tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg cttaatactt
    21781 ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct
    21841 catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct
    21901 tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct
    21961 gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc
    22021 cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt
    22081 cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag
    22141 gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg
    22201 cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc
    22261 ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg
    22321 ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca
    22381 ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat
    22441 ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg
    22501 cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat
    22561 attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca
    22621 gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt
    22681 cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa
    22741 tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt
    22801 tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta
    22861 gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt
    22921 tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt ataatttaac
    22981 agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat
    23041 tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat
    23101 agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg
    23161 cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag
    23221 cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac
    23281 ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc
    23341 tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc
    23401 gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc
    23461 acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga
    23521 tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc
    23581 catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg
    23641 agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga
    23701 cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa
    23761 tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc
    23821 acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc
    23881 attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg
    23941 ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc
    24001 cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg
    24061 atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac
    24121 aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt
    24181 aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt
    24241 ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt
    24301 gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac
    24361 aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt
    24421 gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaaccttaa
    24481 aagatcattt ttttactata tgagcttttt tt
//
LOCUS       DAZBTH010000032        23832 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000032 DAZBTH010000000
VERSION     DAZBTH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.032"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..790)
                     /locus_tag="ACHGNW_001271"
     CDS             complement(56..790)
                     /locus_tag="ACHGNW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6925569.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="ACHGNW_001272"
     CDS             1117..1527
                     /locus_tag="ACHGNW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN6925570.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="ACHGNW_001273"
     CDS             1520..2221
                     /locus_tag="ACHGNW_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN6925571.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="ACHGNW_001274"
     CDS             2221..3807
                     /locus_tag="ACHGNW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925572.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="ACHGNW_001275"
     CDS             3826..4047
                     /locus_tag="ACHGNW_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN6925573.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="ACHGNW_001276"
     CDS             4044..4805
                     /locus_tag="ACHGNW_001276"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN6925574.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="ACHGNW_001277"
     CDS             5185..5694
                     /locus_tag="ACHGNW_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6925575.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="ACHGNW_001278"
     CDS             complement(5769..6308)
                     /locus_tag="ACHGNW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN6925576.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACHGNW_001279"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACHGNW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN6925577.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="ACHGNW_001280"
     CDS             7321..8172
                     /locus_tag="ACHGNW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN6925578.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="ACHGNW_001281"
     CDS             8200..8835
                     /locus_tag="ACHGNW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN6925579.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="ACHGNW_001282"
     CDS             8855..9628
                     /locus_tag="ACHGNW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6925580.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="ACHGNW_001283"
     CDS             10036..10833
                     /locus_tag="ACHGNW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN6925581.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="ACHGNW_001284"
     CDS             10811..11224
                     /locus_tag="ACHGNW_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6925582.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="ACHGNW_001285"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="ACHGNW_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6925583.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="ACHGNW_001286"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="ACHGNW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN6925584.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="ACHGNW_001287"
     CDS             16001..16561
                     /locus_tag="ACHGNW_001287"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN6925585.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACHGNW_001288"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACHGNW_001288"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN6925586.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="ACHGNW_001289"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="ACHGNW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN6925587.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="ACHGNW_001290"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="ACHGNW_001290"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN6925588.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="ACHGNW_001291"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="ACHGNW_001291"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN6925589.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="ACHGNW_001292"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="ACHGNW_001292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN6925590.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="ACHGNW_001293"
     CDS             21908..22486
                     /locus_tag="ACHGNW_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6925591.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="ACHGNW_001294"
     CDS             22538..23020
                     /locus_tag="ACHGNW_001294"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN6925592.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="ACHGNW_001295"
     CDS             23137..23511
                     /locus_tag="ACHGNW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925593.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4285 c         5114 g         6649 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tt
//
LOCUS       DAZBTH010000033        23055 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000033 DAZBTH010000000
VERSION     DAZBTH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.033"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACHGNW_001296"
     CDS             complement(111..566)
                     /locus_tag="ACHGNW_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925594.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACHGNW_001297"
     CDS             complement(720..1928)
                     /locus_tag="ACHGNW_001297"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN6925595.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACHGNW_001298"
     CDS             complement(2040..2372)
                     /locus_tag="ACHGNW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6925596.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACHGNW_001299"
     CDS             2520..3395
                     /locus_tag="ACHGNW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6925597.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACHGNW_001300"
     CDS             complement(3477..4058)
                     /locus_tag="ACHGNW_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN6925598.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACHGNW_001301"
     CDS             complement(4064..5323)
                     /locus_tag="ACHGNW_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6925599.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACHGNW_001302"
     CDS             complement(5325..6371)
                     /locus_tag="ACHGNW_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925600.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACHGNW_001303"
     CDS             complement(6606..6881)
                     /locus_tag="ACHGNW_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN6925601.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHGNW_001304"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHGNW_001304"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN6925602.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHGNW_001305"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHGNW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN6925603.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHGNW_001306"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHGNW_001306"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN6925604.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACHGNW_001307"
     CDS             complement(10709..11344)
                     /locus_tag="ACHGNW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN6925605.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACHGNW_001308"
     CDS             complement(11410..12813)
                     /locus_tag="ACHGNW_001308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN6925606.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACHGNW_001309"
     CDS             complement(12800..13831)
                     /locus_tag="ACHGNW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925607.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACHGNW_001310"
     CDS             13862..14083
                     /locus_tag="ACHGNW_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN6925608.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACHGNW_001311"
     CDS             complement(14248..14985)
                     /locus_tag="ACHGNW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN6925609.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACHGNW_001312"
     CDS             complement(15138..16478)
                     /locus_tag="ACHGNW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6925610.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACHGNW_001313"
     CDS             complement(16715..18238)
                     /locus_tag="ACHGNW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN6925611.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="ACHGNW_001314"
     CDS             complement(18616..19227)
                     /locus_tag="ACHGNW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6925612.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="ACHGNW_001315"
     CDS             complement(19557..20273)
                     /locus_tag="ACHGNW_001315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6925613.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACHGNW_001316"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACHGNW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN6925614.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21602)
                     /locus_tag="ACHGNW_001317"
     CDS             complement(20832..21602)
                     /locus_tag="ACHGNW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN6925615.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="ACHGNW_001318"
     CDS             complement(21599..21970)
                     /locus_tag="ACHGNW_001318"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925616.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACHGNW_001319"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACHGNW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN6925617.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6463 a         4926 c         3908 g         7758 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc atttt
//
LOCUS       DAZBTH010000034        22680 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000034 DAZBTH010000000
VERSION     DAZBTH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.034"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..851
                     /locus_tag="ACHGNW_001320"
     CDS             39..851
                     /locus_tag="ACHGNW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN6925618.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            848..1648
                     /locus_tag="ACHGNW_001321"
     CDS             848..1648
                     /locus_tag="ACHGNW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN6925619.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1809..3077
                     /gene="rseP"
                     /locus_tag="ACHGNW_001322"
     CDS             1809..3077
                     /gene="rseP"
                     /locus_tag="ACHGNW_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN6925620.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3145..4854
                     /locus_tag="ACHGNW_001323"
     CDS             3145..4854
                     /locus_tag="ACHGNW_001323"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN6925621.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5066..9424
                     /locus_tag="ACHGNW_001324"
     CDS             5066..9424
                     /locus_tag="ACHGNW_001324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN6925622.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9569..10042
                     /gene="rimP"
                     /locus_tag="ACHGNW_001325"
     CDS             9569..10042
                     /gene="rimP"
                     /locus_tag="ACHGNW_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN6925623.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10065..11240
                     /gene="nusA"
                     /locus_tag="ACHGNW_001326"
     CDS             10065..11240
                     /gene="nusA"
                     /locus_tag="ACHGNW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN6925624.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11262..11555
                     /locus_tag="ACHGNW_001327"
     CDS             11262..11555
                     /locus_tag="ACHGNW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN6925625.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11552..11863
                     /locus_tag="ACHGNW_001328"
     CDS             11552..11863
                     /locus_tag="ACHGNW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN6925626.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11876..14182
                     /gene="infB"
                     /locus_tag="ACHGNW_001329"
     CDS             11876..14182
                     /gene="infB"
                     /locus_tag="ACHGNW_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN6925627.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14209..14556
                     /gene="rbfA"
                     /locus_tag="ACHGNW_001330"
     CDS             14209..14556
                     /gene="rbfA"
                     /locus_tag="ACHGNW_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN6925628.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14713..15169)
                     /locus_tag="ACHGNW_001331"
                     /pseudo
     CDS             complement(14713..15169)
                     /locus_tag="ACHGNW_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15334..17696
                     /locus_tag="ACHGNW_001332"
                     /pseudo
     CDS             15334..17696
                     /locus_tag="ACHGNW_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18064..18987
                     /gene="truB"
                     /locus_tag="ACHGNW_001333"
     CDS             18064..18987
                     /gene="truB"
                     /locus_tag="ACHGNW_001333"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN6925629.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18991..19932
                     /gene="ribF"
                     /locus_tag="ACHGNW_001334"
     CDS             18991..19932
                     /gene="ribF"
                     /locus_tag="ACHGNW_001334"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN6925630.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20284..21069
                     /locus_tag="ACHGNW_001335"
     CDS             20284..21069
                     /locus_tag="ACHGNW_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6925631.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21082..21933
                     /locus_tag="ACHGNW_001336"
     CDS             21082..21933
                     /locus_tag="ACHGNW_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN6925632.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22057..22509)
                     /locus_tag="ACHGNW_001337"
     CDS             complement(22057..22509)
                     /locus_tag="ACHGNW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925633.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7696 a         3959 c         4958 g         6067 t
ORIGIN      
        1 aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa
       61 aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc
      121 agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac
      181 gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa
      241 ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa
      301 aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag
      361 aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac
      421 atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat
      481 tgaattttgc tttgaattat ggaagcagag cagagatcgt gactgctgtc caagaaattg
      541 ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta
      601 agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta
      661 caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt
      721 attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct
      781 cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg
      841 atcagtcatg aaacaacgtg tggtcacagc agccgtggcg ctagtcttgt ttattccgat
      901 tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcag taataggagt
      961 ttacgagctg tttcggatga aaggattgac actcttaagt tttgaaggag ttttatccgc
     1021 cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa
     1081 tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat
     1141 ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt
     1201 gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt
     1261 tggtctattt attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg
     1321 tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg
     1381 tattggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact
     1441 atttaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca
     1501 atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa
     1561 tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc
     1621 tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat
     1681 aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat
     1741 ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga
     1801 aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc
     1861 atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta
     1921 tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc
     1981 gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa
     2041 tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa
     2101 atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt
     2161 tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct
     2221 accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc
     2281 caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg
     2341 gacccatgaa taactttatt ttggcaatcc tgttattcat cattctggca tttatgcaag
     2401 gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg
     2461 cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt
     2521 catggaatga tctgacaaca gtcattacga aaaatccgga taaaacatta gactttaaga
     2581 tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg
     2641 gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga
     2701 tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat
     2761 cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt
     2821 cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa
     2881 tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct
     2941 tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga
     3001 cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc
     3061 aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt
     3121 tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc
     3181 gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc
     3241 cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa
     3301 ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct
     3361 gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac
     3421 ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa
     3481 acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt
     3541 tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga
     3601 agagagttta tcatgaaaga tgcgtattct ttccatgcaa cggaagaaag tttggacgag
     3661 acatataaag attacgaaaa agcctacacc gaagtattca agcgttgtgg cttagaattt
     3721 cgctcgatta tcggagacgg tggcgcaatg ggcgggaaag actcaaaaga attcatggcg
     3781 atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac
     3841 ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg
     3901 gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt
     3961 gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt
     4021 ttggtacgcg gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac
     4081 tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct
     4141 gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat
     4201 ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca
     4261 gaacgcgatt tcactgtact ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct
     4321 tcaccagaca acaatggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatcttc
     4381 aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga
     4441 gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc
     4501 gtagaacaac attcagatga acaaggaatc aactggccaa aaggaattgc gccatttgat
     4561 ttgcatatcg tccaaatgaa tctaaaagac gaataccaaa caaacctgac gaatgaacta
     4621 gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca
     4681 ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa
     4741 aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta
     4801 cgtaaagaag aattggtgaa tactttacca atcctcttga accaaggcga ataataagcg
     4861 ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct
     4921 tttaaaattg gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa
     4981 aaacgagtat actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt
     5041 ttctcatcag aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt
     5101 ggaacaaatc caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat
     5161 ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa tttacgctgc atttttccaa
     5221 aattttgcct attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga
     5281 tattgcacaa gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt
     5341 gcaagattac tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca
     5401 agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag
     5461 tacaggagct atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt
     5521 cagttacgga ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg
     5581 agtattgaaa ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc
     5641 agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc
     5701 tcttgaagga cccatccaca tccagctagg acgaaatatt ccagccgatg aaccaacgac
     5761 accaatgatc agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga
     5821 caaagaagtc agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta
     5881 tacttcatca tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga
     5941 tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt
     6001 ccgtgatttg gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga
     6061 ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat
     6121 ggatgcaaca aacagcatct ctgatcttgt ggcacaagca gggaaatggg gacaccgagc
     6181 tatcgccatt acagaccacg gtggtgcgca agcctttccg gaagcacata gtgctggaaa
     6241 aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc
     6301 aattgcttat aatgatgcac atgaagcatt gagtgaagcc acatatgtcg tatttgacgt
     6361 ggaaacgaca ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat
     6421 gtataaagga aatgtgatcg aaagctttga tgaatttatc gatccaggac atcctttgtc
     6481 acgaacaacg gtagatctga ctggtatcac ggatggaatg gttcgaggat caaagtcgga
     6541 agaagaagta ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa
     6601 cgcggctttt gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc
     6661 ggccaatcca gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg
     6721 tttcggatta ggtgtgttat ctaaaaaatt tggtgtcagc ttggaacagc atcaccgagc
     6781 aatctatgat gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga
     6841 caatcataac atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta
     6901 caaaagggct cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga
     6961 tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc
     7021 acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg
     7081 agaaatattc gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt
     7141 ctacgattat attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt
     7201 ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag
     7261 tttggataaa atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat
     7321 ttaccgaaaa attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt
     7381 gccggatgtc catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga
     7441 agaaacagcg aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga
     7501 agtgattcca gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat
     7561 atcagaattg agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat
     7621 tcaaaaacga ttaaaaaaag agttgaattc tattaatggt aatggcttct cggttattta
     7681 tctgatcgcc caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg
     7741 tggctctgtt ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccgtt
     7801 agcccctcat tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta
     7861 tggttctgga tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa
     7921 agacggtcat gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga
     7981 tattgatttg aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt
     8041 gtttggcgaa gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc
     8101 atatggctat gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt
     8161 cgacagatta gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg
     8221 gatcatcgtt attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc
     8281 agacgatcaa aattcggaat ggaaaacgac tcattttgat tttcactcga ttcacgataa
     8341 tgtattgaaa ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga
     8401 tttatcaggg atcgatccac aaacgatccc tacggatgac ccggaagtga tgcggatatt
     8461 tgccggtcct gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt
     8521 aggtattcca gaattcggca ctcgttttgt tcgagggatg ctagaagaaa cgcatcctac
     8581 aacttttgca gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg
     8641 aaatgcggaa gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg
     8701 tgatgatatt atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat
     8761 catggaaact gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta
     8821 tctatcggcg atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat
     8881 caaatatatg tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc
     8941 ttatttcaag gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga
     9001 cgactttgat ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga
     9061 aatcacggac aaaggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga
     9121 gttagccaat gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc
     9181 ggatgccgtt aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc
     9241 aagtttggga accaacgtgg caaaacaaat cgttgaagca agaaaagacg gtccattttt
     9301 atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa
     9361 cgacaatggc gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct
     9421 ataagcatct gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata
     9481 ggtaatgata ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg
     9541 cacttaattt tttaaggagg cgaaactttt gagtagcgtc gttgaaacag tcacagaaat
     9601 ggtgacaccg attttagatg aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga
     9661 aggaaaaagc tggttcttac gagtatttat tgacaaagaa ggcggtatcg atatcgaaga
     9721 atgcgccttt gtcagtgaga aactgagtga aaaacttgat gcagcagatc cagatccgat
     9781 tcctcaagct tatttcctgg aggtatcctc cccgggagca gagcggccat tgaaaaaaga
     9841 aagcgactat gaaaaggcac ttggtgatta tgtccatgtc tccttatacc aaccagtaga
     9901 tggcgaaaaa caatatgaag gatttcttca atcatttgat gctgaacagc ttacattgaa
     9961 aatacgcata aaaacacgag aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc
    10021 tcgcttagct atccaatttt aatcacggag aggaatcaga aaaaatgagt aaagaaatgt
    10081 taaatgcgtt agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg
    10141 ctttagaagc agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg
    10201 aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg
    10261 aagaagtgat ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag
    10321 catatgagat cggtgatacg atccgttttg aagtaacacc aaaagatttt gggcggatcg
    10381 ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta
    10441 tttataacga attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg
    10501 ataaacgtta tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc
    10561 aaatgccaaa tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag
    10621 aaaatacgtc taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgcc
    10681 gcttatttga gcaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg
    10741 cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg
    10801 cagtcggtac atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa
    10861 aaggcgaaaa catggatatc gtcgaatgga atgaagatcc agctgtattc attgcaaatg
    10921 cattgaatcc tgcacaagtc atggatgtga tcttcgatga gacaaatcca aaggcctgca
    10981 cagttgtcgt tccagactac caattatcgc tagcaatcgg taaacgcgga caaaacgcac
    11041 gtttagctgc taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg
    11101 aattttatga aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg
    11161 ttcagtcaga tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg
    11221 aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc
    11281 ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca
    11341 cccgttcaaa agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag
    11401 cgtatgtctc acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc
    11461 gagtactcga aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc
    11521 accaaaaagc acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat
    11581 gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat
    11641 tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagcgagaa
    11701 tacaagaaaa aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt
    11761 ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga
    11821 tgccggattt gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg
    11881 caacaaaaga atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga
    11941 aaaagcacaa caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcaatga
    12001 tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca
    12061 agcaagcaga aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa
    12121 caatcaacaa aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca
    12181 agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca
    12241 aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc
    12301 aaacaataac aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg
    12361 taaaaaaggc aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga
    12421 attgccagat gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat
    12481 tcatcgtgag ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca
    12541 aaaccaagca ctggataaag atacgattga actgttagcg acggactatg gcatggaacc
    12601 acaagaaaaa gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt
    12661 cgatccagat aaattagtct cacgtccgcc agtcgtaacg attatgggac acgtagacca
    12721 tgggaaaaca actttattgg atacattacg ccattcacgt gtaacaagcg gcgaagccgg
    12781 cggaatcaca cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt
    12841 cttggataca ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac
    12901 agatatcacg atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc
    12961 aatcaaccat gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa
    13021 accaggtgca aaccctcaac atgttatgca agaattaagt gagtatgagt taattcctga
    13081 agcatggggt ggagaaacga tctttgttga gatctcagcg aaattcggcc aaaacatcga
    13141 agaattgctg gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac
    13201 acaacgcgca atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc
    13261 aaccttactt gtccaagaag gaacacttca tgttggtgac ccaatcgttg tcggtaatac
    13321 ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc
    13381 tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt
    13441 agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcga aacgtgcatt
    13501 attagaaaac cgtgctgcca caagccgtgt gactttggat aacttatttg aaagcctgaa
    13561 agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga
    13621 agcactggct gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca
    13681 ttcagctgtc ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat
    13741 tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga
    13801 agtcgatatt cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat
    13861 gaaagggatg ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga
    13921 aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat
    13981 tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact
    14041 tgcaagtctg aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg
    14101 tgcaatgatt gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat
    14161 ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac
    14221 cgtgaccgcc gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag
    14281 gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta
    14341 cagcaagcaa cgatctatta cagcatttta tctgacttag catcagataa gaaaacagca
    14401 caagatggat tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt
    14461 atttataaga cacctgaatt gttctttgaa cttgatgaat cagttgtcta tggcaaccat
    14521 atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa aaactcccgc aaatcctgtg
    14581 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
    14641 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
    14701 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
    14761 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
    14821 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
    14881 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
    14941 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
    15001 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
    15061 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
    15121 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
    15181 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
    15241 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
    15301 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
    15361 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
    15421 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
    15481 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
    15541 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
    15601 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
    15661 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
    15721 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
    15781 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
    15841 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
    15901 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
    15961 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
    16021 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
    16081 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
    16141 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
    16201 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
    16261 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
    16321 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
    16381 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
    16441 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
    16501 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
    16561 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
    16621 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
    16681 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
    16741 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
    16801 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
    16861 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
    16921 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
    16981 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
    17041 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
    17101 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
    17161 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
    17221 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
    17281 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
    17341 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
    17401 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
    17461 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
    17521 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
    17581 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
    17641 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
    17701 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
    17761 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
    17821 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
    17881 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
    17941 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
    18001 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
    18061 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
    18121 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
    18181 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
    18241 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
    18301 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
    18361 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
    18421 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
    18481 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
    18541 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
    18601 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
    18661 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
    18721 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
    18781 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
    18841 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
    18901 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
    18961 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
    19021 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
    19081 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
    19141 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
    19201 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
    19261 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
    19321 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
    19381 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
    19441 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
    19501 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
    19561 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
    19621 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
    19681 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
    19741 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
    19801 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
    19861 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
    19921 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
    19981 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
    20041 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
    20101 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
    20161 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
    20221 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
    20281 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
    20341 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
    20401 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
    20461 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
    20521 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
    20581 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
    20641 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
    20701 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
    20761 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
    20821 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
    20881 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
    20941 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
    21001 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
    21061 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
    21121 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
    21181 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
    21241 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
    21301 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
    21361 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
    21421 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
    21481 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
    21541 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
    21601 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
    21661 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
    21721 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
    21781 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
    21841 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
    21901 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
    21961 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
    22021 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
    22081 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
    22141 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
    22201 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
    22261 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
    22321 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
    22381 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
    22441 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
    22501 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
    22561 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
    22621 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
//
LOCUS       DAZBTH010000035        22031 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000035 DAZBTH010000000
VERSION     DAZBTH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.035"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHGNW_001338"
     CDS             367..555
                     /locus_tag="ACHGNW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925634.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHGNW_001339"
     CDS             545..805
                     /locus_tag="ACHGNW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFN6925635.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHGNW_001340"
     CDS             846..1073
                     /locus_tag="ACHGNW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925636.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHGNW_001341"
     CDS             1138..1710
                     /locus_tag="ACHGNW_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925637.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHGNW_001342"
     CDS             1711..2022
                     /locus_tag="ACHGNW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6925638.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHGNW_001343"
     CDS             2013..2273
                     /locus_tag="ACHGNW_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925639.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHGNW_001344"
     CDS             2434..2793
                     /locus_tag="ACHGNW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925640.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHGNW_001345"
     CDS             2790..4433
                     /locus_tag="ACHGNW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6925641.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHGNW_001346"
     CDS             4468..5550
                     /locus_tag="ACHGNW_001346"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFN6925642.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHGNW_001347"
     CDS             5594..6766
                     /locus_tag="ACHGNW_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6925643.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHGNW_001348"
     CDS             6753..7457
                     /locus_tag="ACHGNW_001348"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6925644.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHGNW_001349"
     CDS             7462..8622
                     /locus_tag="ACHGNW_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6925645.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHGNW_001350"
     CDS             8698..8976
                     /locus_tag="ACHGNW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFN6925646.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHGNW_001351"
     CDS             8957..9328
                     /locus_tag="ACHGNW_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFN6925647.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHGNW_001352"
     CDS             9344..9748
                     /locus_tag="ACHGNW_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925648.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHGNW_001353"
     CDS             9745..10155
                     /locus_tag="ACHGNW_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925649.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHGNW_001354"
     CDS             10216..10803
                     /locus_tag="ACHGNW_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6925650.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHGNW_001355"
     CDS             10876..11343
                     /locus_tag="ACHGNW_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFN6925651.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHGNW_001356"
     CDS             11389..11697
                     /locus_tag="ACHGNW_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925652.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHGNW_001357"
     CDS             11892..18626
                     /locus_tag="ACHGNW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN6925653.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACHGNW_001358"
     CDS             18657..19502
                     /locus_tag="ACHGNW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN6925654.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACHGNW_001359"
     CDS             19499..20548
                     /locus_tag="ACHGNW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925655.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHGNW_001360"
     CDS             20552..21376
                     /locus_tag="ACHGNW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925656.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHGNW_001361"
     CDS             21394..>22031
                     /locus_tag="ACHGNW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN6925657.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAZBTH010000036        22028 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000036 DAZBTH010000000
VERSION     DAZBTH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.036"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACHGNW_001362"
     CDS             complement(61..1371)
                     /locus_tag="ACHGNW_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN6925658.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACHGNW_001363"
     CDS             complement(1383..2801)
                     /locus_tag="ACHGNW_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925659.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACHGNW_001364"
     CDS             complement(3041..4609)
                     /locus_tag="ACHGNW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN6925660.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACHGNW_001365"
     CDS             complement(4613..4894)
                     /locus_tag="ACHGNW_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN6925661.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACHGNW_001366"
     CDS             complement(4891..5814)
                     /locus_tag="ACHGNW_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN6925662.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACHGNW_001367"
     CDS             complement(5811..6689)
                     /locus_tag="ACHGNW_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN6925663.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACHGNW_001368"
     CDS             complement(6676..8013)
                     /locus_tag="ACHGNW_001368"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN6925664.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACHGNW_001369"
     CDS             complement(8016..9119)
                     /locus_tag="ACHGNW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6925665.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACHGNW_001370"
     CDS             complement(9193..10701)
                     /locus_tag="ACHGNW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6925666.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACHGNW_001371"
     CDS             complement(10667..11359)
                     /locus_tag="ACHGNW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN6925667.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACHGNW_001372"
     CDS             complement(11381..12355)
                     /locus_tag="ACHGNW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925668.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACHGNW_001373"
     CDS             12655..13527
                     /locus_tag="ACHGNW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925669.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACHGNW_001374"
     CDS             complement(14109..16307)
                     /locus_tag="ACHGNW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925670.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ACHGNW_001375"
     CDS             16539..17531
                     /locus_tag="ACHGNW_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6925671.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ACHGNW_001376"
     CDS             17591..19012
                     /locus_tag="ACHGNW_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN6925672.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ACHGNW_001377"
     CDS             19005..20504
                     /locus_tag="ACHGNW_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN6925673.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHGNW_001378"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHGNW_001378"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6925674.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAZBTH010000037        21988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000037 DAZBTH010000000
VERSION     DAZBTH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.037"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHGNW_001379"
     CDS             <1..395
                     /locus_tag="ACHGNW_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN6925675.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHGNW_001380"
     CDS             398..1147
                     /locus_tag="ACHGNW_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925676.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHGNW_001381"
     CDS             1159..1428
                     /locus_tag="ACHGNW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925677.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLKDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHGNW_001382"
     CDS             1433..1597
                     /locus_tag="ACHGNW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925678.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHGNW_001383"
     CDS             1590..1892
                     /locus_tag="ACHGNW_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925679.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHGNW_001384"
     CDS             1889..2050
                     /locus_tag="ACHGNW_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6925680.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHGNW_001385"
     CDS             2047..2352
                     /locus_tag="ACHGNW_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925681.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHGNW_001386"
     CDS             2352..2708
                     /locus_tag="ACHGNW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFN6925682.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHGNW_001387"
     CDS             2668..2913
                     /locus_tag="ACHGNW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925683.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHGNW_001388"
     CDS             2910..3329
                     /locus_tag="ACHGNW_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN6925684.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHGNW_001389"
     CDS             3326..3883
                     /locus_tag="ACHGNW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFN6925685.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHGNW_001390"
     CDS             3880..4176
                     /locus_tag="ACHGNW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925686.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHGNW_001391"
     CDS             4253..4666
                     /locus_tag="ACHGNW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6925687.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHGNW_001392"
     CDS             5012..5386
                     /locus_tag="ACHGNW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925688.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHGNW_001393"
     CDS             5423..5590
                     /locus_tag="ACHGNW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925689.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHGNW_001394"
     CDS             5616..5960
                     /locus_tag="ACHGNW_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN6925690.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHGNW_001395"
     CDS             5965..6246
                     /locus_tag="ACHGNW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925691.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHGNW_001396"
     CDS             6349..6663
                     /locus_tag="ACHGNW_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN6925692.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHGNW_001397"
     CDS             6641..8335
                     /locus_tag="ACHGNW_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6925693.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHGNW_001398"
     CDS             8355..9533
                     /locus_tag="ACHGNW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6925694.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHGNW_001399"
     CDS             9496..10182
                     /locus_tag="ACHGNW_001399"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6925695.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHGNW_001400"
     CDS             10182..11342
                     /locus_tag="ACHGNW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6925696.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHGNW_001401"
     CDS             11352..12227
                     /locus_tag="ACHGNW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925697.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHGNW_001402"
     CDS             12224..12535
                     /locus_tag="ACHGNW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925698.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHGNW_001403"
     CDS             12525..12878
                     /locus_tag="ACHGNW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6925699.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHGNW_001404"
     CDS             12868..13269
                     /locus_tag="ACHGNW_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925700.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHGNW_001405"
     CDS             13262..13666
                     /locus_tag="ACHGNW_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925701.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHGNW_001406"
     CDS             13678..14286
                     /locus_tag="ACHGNW_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6925702.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHGNW_001407"
     CDS             14306..14668
                     /locus_tag="ACHGNW_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925703.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHGNW_001408"
     CDS             14671..14853
                     /locus_tag="ACHGNW_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925704.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHGNW_001409"
     CDS             14870..18289
                     /locus_tag="ACHGNW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN6925705.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHGNW_001410"
     CDS             18340..19077
                     /locus_tag="ACHGNW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6925706.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHGNW_001411"
     CDS             19087..21378
                     /locus_tag="ACHGNW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6925707.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACHGNW_001412"
     CDS             21402..>21988
                     /locus_tag="ACHGNW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN6925708.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7934 a         3245 c         4704 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg aaagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZBTH010000038        21912 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000038 DAZBTH010000000
VERSION     DAZBTH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.038"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHGNW_001413"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHGNW_001413"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN6925709.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHGNW_001414"
     CDS             complement(1172..1867)
                     /locus_tag="ACHGNW_001414"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN6925710.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHGNW_001415"
     CDS             complement(1880..2596)
                     /locus_tag="ACHGNW_001415"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN6925711.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHGNW_001416"
     CDS             2926..3534
                     /locus_tag="ACHGNW_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN6925712.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHGNW_001417"
     CDS             3859..4182
                     /locus_tag="ACHGNW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN6925713.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHGNW_001418"
     CDS             4364..5734
                     /locus_tag="ACHGNW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN6925714.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHGNW_001419"
     CDS             complement(5864..7165)
                     /locus_tag="ACHGNW_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6925715.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHGNW_001420"
     CDS             complement(7519..9510)
                     /locus_tag="ACHGNW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN6925716.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHGNW_001421"
     CDS             complement(9654..9920)
                     /locus_tag="ACHGNW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6925717.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHGNW_001422"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHGNW_001422"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN6925718.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHGNW_001423"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHGNW_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN6925719.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHGNW_001424"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHGNW_001424"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6925720.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHGNW_001425"
     CDS             complement(14080..14601)
                     /locus_tag="ACHGNW_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN6925721.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHGNW_001426"
     CDS             complement(14604..15446)
                     /locus_tag="ACHGNW_001426"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN6925722.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHGNW_001427"
     CDS             complement(15439..16164)
                     /locus_tag="ACHGNW_001427"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN6925723.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHGNW_001428"
     CDS             16340..16636
                     /locus_tag="ACHGNW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925724.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHGNW_001429"
     CDS             17204..18796
                     /locus_tag="ACHGNW_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6925725.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHGNW_001430"
     CDS             18809..19549
                     /locus_tag="ACHGNW_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6925726.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHGNW_001431"
     CDS             19717..21066
                     /locus_tag="ACHGNW_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6925727.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHGNW_001432"
     CDS             complement(21194..21784)
                     /locus_tag="ACHGNW_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6925728.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAZBTH010000039        21882 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000039 DAZBTH010000000
VERSION     DAZBTH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.039"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACHGNW_001433"
     CDS             complement(205..1104)
                     /locus_tag="ACHGNW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6925729.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACHGNW_001434"
     CDS             complement(1114..1470)
                     /locus_tag="ACHGNW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6925730.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACHGNW_001435"
     CDS             1825..2121
                     /locus_tag="ACHGNW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN6925731.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACHGNW_001436"
     CDS             complement(2765..3718)
                     /locus_tag="ACHGNW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6925732.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACHGNW_001437"
     CDS             complement(3741..4496)
                     /locus_tag="ACHGNW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925733.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACHGNW_001438"
     CDS             complement(4493..5452)
                     /locus_tag="ACHGNW_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6925734.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACHGNW_001439"
     CDS             complement(5445..6395)
                     /locus_tag="ACHGNW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6925735.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACHGNW_001440"
     CDS             6545..6997
                     /locus_tag="ACHGNW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN6925736.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHGNW_001441"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHGNW_001441"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN6925737.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHGNW_001442"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHGNW_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN6925738.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACHGNW_001443"
     CDS             complement(10549..11358)
                     /locus_tag="ACHGNW_001443"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6925739.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHGNW_001444"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHGNW_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN6925740.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHGNW_001445"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHGNW_001445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN6925741.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACHGNW_001446"
     CDS             complement(16002..17783)
                     /locus_tag="ACHGNW_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6925742.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACHGNW_001447"
     CDS             complement(17809..18723)
                     /locus_tag="ACHGNW_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925743.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHGNW_001448"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHGNW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6925744.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACHGNW_001449"
     CDS             complement(19712..20656)
                     /locus_tag="ACHGNW_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925745.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACHGNW_001450"
     CDS             complement(20657..21661)
                     /locus_tag="ACHGNW_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6925746.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6131 a         4656 c         3866 g         7229 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg at
//
LOCUS       DAZBTH010000040        20586 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000040 DAZBTH010000000
VERSION     DAZBTH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.040"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACHGNW_001451"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACHGNW_001451"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN6925747.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACHGNW_001452"
     CDS             1011..2258
                     /locus_tag="ACHGNW_001452"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN6925748.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACHGNW_001453"
     CDS             2334..2678
                     /locus_tag="ACHGNW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925749.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACHGNW_001454"
     CDS             complement(2795..3121)
                     /locus_tag="ACHGNW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925750.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ACHGNW_001455"
     CDS             complement(3736..4938)
                     /locus_tag="ACHGNW_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925751.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ACHGNW_001456"
     CDS             5055..5570
                     /locus_tag="ACHGNW_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6925752.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ACHGNW_001457"
     CDS             complement(5769..6215)
                     /locus_tag="ACHGNW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6925753.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACHGNW_001458"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACHGNW_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6925754.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ACHGNW_001459"
     CDS             complement(8094..8723)
                     /locus_tag="ACHGNW_001459"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6925755.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="ACHGNW_001460"
     CDS             complement(8707..9171)
                     /locus_tag="ACHGNW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6925756.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="ACHGNW_001461"
     CDS             complement(9658..10926)
                     /locus_tag="ACHGNW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6925757.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="ACHGNW_001462"
     CDS             complement(11157..11894)
                     /locus_tag="ACHGNW_001462"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6925758.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="ACHGNW_001463"
     CDS             complement(11961..12530)
                     /locus_tag="ACHGNW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN6925759.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="ACHGNW_001464"
     CDS             12658..13530
                     /locus_tag="ACHGNW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6925760.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="ACHGNW_001465"
     CDS             13872..14744
                     /locus_tag="ACHGNW_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6925761.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /gene="nagE"
                     /locus_tag="ACHGNW_001466"
     CDS             14741..16699
                     /gene="nagE"
                     /locus_tag="ACHGNW_001466"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN6925762.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="ACHGNW_001467"
     CDS             complement(17031..17882)
                     /locus_tag="ACHGNW_001467"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6925763.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="ACHGNW_001468"
     CDS             18005..18472
                     /locus_tag="ACHGNW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN6925764.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="ACHGNW_001469"
     CDS             complement(18735..19217)
                     /locus_tag="ACHGNW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6925765.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="ACHGNW_001470"
     CDS             complement(19317..20180)
                     /locus_tag="ACHGNW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6925766.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6197 a         4227 c         3724 g         6438 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaa
//
LOCUS       DAZBTH010000041        20475 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000041 DAZBTH010000000
VERSION     DAZBTH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.041"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACHGNW_001471"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACHGNW_001471"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN6925767.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACHGNW_001472"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACHGNW_001472"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN6925768.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACHGNW_001473"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACHGNW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN6925769.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACHGNW_001474"
     CDS             2857..3732
                     /locus_tag="ACHGNW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN6925770.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHGNW_001475"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHGNW_001475"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN6925771.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACHGNW_001476"
     CDS             4629..5063
                     /locus_tag="ACHGNW_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN6925772.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACHGNW_001477"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACHGNW_001477"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN6925773.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACHGNW_001478"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACHGNW_001478"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN6925774.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACHGNW_001479"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACHGNW_001479"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN6925775.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACHGNW_001480"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACHGNW_001480"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN6925776.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACHGNW_001481"
     CDS             13334..14260
                     /locus_tag="ACHGNW_001481"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN6925777.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACHGNW_001482"
     CDS             14442..15335
                     /locus_tag="ACHGNW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN6925778.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACHGNW_001483"
     CDS             15469..15594
                     /locus_tag="ACHGNW_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN6925779.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACHGNW_001484"
     CDS             complement(15728..16360)
                     /locus_tag="ACHGNW_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925780.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ACHGNW_001485"
     CDS             complement(16508..17050)
                     /locus_tag="ACHGNW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN6925781.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ACHGNW_001486"
     CDS             17185..17574
                     /locus_tag="ACHGNW_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925782.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ACHGNW_001487"
     CDS             17578..18342
                     /locus_tag="ACHGNW_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6925783.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ACHGNW_001488"
     CDS             18471..19418
                     /locus_tag="ACHGNW_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6925784.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ACHGNW_001489"
     CDS             19548..20288
                     /locus_tag="ACHGNW_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925785.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7187 a         3481 c         4315 g         5492 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgc
//
LOCUS       DAZBTH010000042        20144 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000042 DAZBTH010000000
VERSION     DAZBTH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.042"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHGNW_001490"
     CDS             <1..152
                     /locus_tag="ACHGNW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925786.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACHGNW_001491"
     CDS             complement(230..763)
                     /locus_tag="ACHGNW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN6925787.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHGNW_001492"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHGNW_001492"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN6925788.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHGNW_001493"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHGNW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN6925789.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHGNW_001494"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHGNW_001494"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6925790.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACHGNW_001495"
     CDS             complement(4488..5066)
                     /locus_tag="ACHGNW_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN6925791.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACHGNW_001496"
     CDS             5388..5858
                     /locus_tag="ACHGNW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN6925792.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACHGNW_001497"
     CDS             complement(5966..7192)
                     /locus_tag="ACHGNW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6925793.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACHGNW_001498"
     CDS             complement(7946..8146)
                     /locus_tag="ACHGNW_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6925794.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACHGNW_001499"
     CDS             complement(8280..8888)
                     /locus_tag="ACHGNW_001499"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN6925795.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACHGNW_001500"
     CDS             complement(8973..9443)
                     /locus_tag="ACHGNW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN6925796.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACHGNW_001501"
     CDS             complement(9491..10405)
                     /locus_tag="ACHGNW_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6925797.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACHGNW_001502"
     CDS             10551..11354
                     /locus_tag="ACHGNW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN6925798.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACHGNW_001503"
     CDS             11532..12479
                     /locus_tag="ACHGNW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN6925799.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACHGNW_001504"
     CDS             complement(12547..12840)
                     /locus_tag="ACHGNW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN6925800.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACHGNW_001505"
     CDS             complement(12868..13533)
                     /locus_tag="ACHGNW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN6925801.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACHGNW_001506"
     CDS             complement(13673..14305)
                     /locus_tag="ACHGNW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6925802.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHGNW_001507"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHGNW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN6925803.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHGNW_001508"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHGNW_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN6925804.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHGNW_001509"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHGNW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN6925805.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHGNW_001510"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHGNW_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN6925806.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ACHGNW_001511"
     CDS             18270..19439
                     /locus_tag="ACHGNW_001511"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6925807.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20144)
                     /locus_tag="ACHGNW_001512"
     CDS             complement(19706..>20144)
                     /locus_tag="ACHGNW_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925808.1"
                     /translation="GTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI
                     YSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSES
                     GINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6094 a         4018 c         3207 g         6825 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tcct
//
LOCUS       DAZBTH010000043        19126 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000043 DAZBTH010000000
VERSION     DAZBTH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.043"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHGNW_001513"
     CDS             complement(<1..703)
                     /locus_tag="ACHGNW_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6925809.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHGNW_001514"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHGNW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHGNW_001515"
     CDS             1758..2837
                     /locus_tag="ACHGNW_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6925810.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHGNW_001516"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHGNW_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHGNW_001517"
     CDS             complement(3572..3799)
                     /locus_tag="ACHGNW_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6925811.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHGNW_001518"
     CDS             complement(3902..6490)
                     /locus_tag="ACHGNW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN6925812.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHGNW_001519"
     CDS             complement(6510..7169)
                     /locus_tag="ACHGNW_001519"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6925813.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHGNW_001520"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHGNW_001520"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN6925814.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHGNW_001521"
     CDS             complement(7942..8808)
                     /locus_tag="ACHGNW_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6925815.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHGNW_001522"
     CDS             8917..9558
                     /locus_tag="ACHGNW_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN6925816.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHGNW_001523"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHGNW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN6925817.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHGNW_001524"
     CDS             complement(10999..11898)
                     /locus_tag="ACHGNW_001524"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6925818.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHGNW_001525"
     CDS             complement(11900..12697)
                     /locus_tag="ACHGNW_001525"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN6925819.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHGNW_001526"
     CDS             complement(12675..13379)
                     /locus_tag="ACHGNW_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN6925820.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHGNW_001527"
     CDS             13510..14085
                     /locus_tag="ACHGNW_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN6925821.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHGNW_001528"
     CDS             14203..14880
                     /locus_tag="ACHGNW_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN6925822.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHGNW_001529"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHGNW_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN6925823.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHGNW_001530"
     CDS             complement(16943..17941)
                     /locus_tag="ACHGNW_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN6925824.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHGNW_001531"
     CDS             complement(18167..18820)
                     /locus_tag="ACHGNW_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN6925825.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAZBTH010000044        18989 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000044 DAZBTH010000000
VERSION     DAZBTH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.044"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACHGNW_001532"
     CDS             complement(17..538)
                     /locus_tag="ACHGNW_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN6925826.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACHGNW_001533"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACHGNW_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN6925827.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHGNW_001534"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHGNW_001534"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN6925828.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACHGNW_001535"
     CDS             5502..6671
                     /locus_tag="ACHGNW_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN6925829.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /gene="glmM"
                     /locus_tag="ACHGNW_001536"
     CDS             6695..8050
                     /gene="glmM"
                     /locus_tag="ACHGNW_001536"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN6925830.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHGNW_001537"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHGNW_001537"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN6925831.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="ACHGNW_001538"
     CDS             10336..10626
                     /locus_tag="ACHGNW_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925832.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="ACHGNW_001539"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="ACHGNW_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN6925833.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="ACHGNW_001540"
     CDS             11667..12326
                     /locus_tag="ACHGNW_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN6925834.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="ACHGNW_001541"
     CDS             12483..13415
                     /locus_tag="ACHGNW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925835.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /gene="tig"
                     /locus_tag="ACHGNW_001542"
     CDS             13569..14858
                     /gene="tig"
                     /locus_tag="ACHGNW_001542"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN6925836.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="ACHGNW_001543"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="ACHGNW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN6925837.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /gene="yihA"
                     /locus_tag="ACHGNW_001544"
     CDS             16438..17025
                     /gene="yihA"
                     /locus_tag="ACHGNW_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN6925838.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="ACHGNW_001545"
     CDS             complement(17099..17254)
                     /locus_tag="ACHGNW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN6925839.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18718
                     /locus_tag="ACHGNW_001546"
     CDS             17390..18718
                     /locus_tag="ACHGNW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925840.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18729..18938
                     /locus_tag="ACHGNW_001547"
     CDS             18729..18938
                     /locus_tag="ACHGNW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925841.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6219 a         3380 c         4086 g         5304 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattata ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    18901 atatgaaaaa aacaagaagc gaaaataaaa aacgataaaa cggcttgatg atagacaaaa
    18961 aaataaaaaa ccagtctttc tgactggtt
//
LOCUS       DAZBTH010000045        18741 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000045 DAZBTH010000000
VERSION     DAZBTH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.045"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1774
                     /locus_tag="ACHGNW_001548"
     CDS             89..1774
                     /locus_tag="ACHGNW_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6925842.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1788..3071
                     /locus_tag="ACHGNW_001549"
     CDS             1788..3071
                     /locus_tag="ACHGNW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6925843.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3141..4010
                     /locus_tag="ACHGNW_001550"
     CDS             3141..4010
                     /locus_tag="ACHGNW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925844.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4007..4843
                     /locus_tag="ACHGNW_001551"
     CDS             4007..4843
                     /locus_tag="ACHGNW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925845.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4869..5936
                     /locus_tag="ACHGNW_001552"
     CDS             4869..5936
                     /locus_tag="ACHGNW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN6925846.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5933..6760
                     /locus_tag="ACHGNW_001553"
     CDS             5933..6760
                     /locus_tag="ACHGNW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6925847.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6805..7830
                     /locus_tag="ACHGNW_001554"
     CDS             6805..7830
                     /locus_tag="ACHGNW_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6925848.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7827..10010
                     /locus_tag="ACHGNW_001555"
     CDS             7827..10010
                     /locus_tag="ACHGNW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN6925849.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10010..11140
                     /locus_tag="ACHGNW_001556"
     CDS             10010..11140
                     /locus_tag="ACHGNW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6925850.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11137..11814
                     /gene="pgmB"
                     /locus_tag="ACHGNW_001557"
     CDS             11137..11814
                     /gene="pgmB"
                     /locus_tag="ACHGNW_001557"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6925851.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11898..12425
                     /gene="msrA"
                     /locus_tag="ACHGNW_001558"
     CDS             11898..12425
                     /gene="msrA"
                     /locus_tag="ACHGNW_001558"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6925852.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12626..13312)
                     /locus_tag="ACHGNW_001559"
     CDS             complement(12626..13312)
                     /locus_tag="ACHGNW_001559"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN6925853.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13392..14930
                     /locus_tag="ACHGNW_001560"
     CDS             13392..14930
                     /locus_tag="ACHGNW_001560"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN6925854.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15050..15517
                     /locus_tag="ACHGNW_001561"
     CDS             15050..15517
                     /locus_tag="ACHGNW_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN6925855.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16051..16479
                     /gene="rplM"
                     /locus_tag="ACHGNW_001562"
     CDS             16051..16479
                     /gene="rplM"
                     /locus_tag="ACHGNW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN6925856.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16495..16887
                     /gene="rpsI"
                     /locus_tag="ACHGNW_001563"
     CDS             16495..16887
                     /gene="rpsI"
                     /locus_tag="ACHGNW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6925857.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16975..18201)
                     /locus_tag="ACHGNW_001564"
     CDS             complement(16975..18201)
                     /locus_tag="ACHGNW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925858.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(18273..18575)
                     /locus_tag="ACHGNW_001565"
     CDS             complement(18273..18575)
                     /locus_tag="ACHGNW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925859.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6066 a         3415 c         3948 g         5312 t
ORIGIN      
        1 ttatttaaaa aaatgctatt gacagtaagc gttttccata atatgatcaa atcgttggga
       61 acgctcccaa aaaagaaagg ggaatccgat gtatacaaaa cttttacaag cgatttatcg
      121 cgactcagat ttgtctgaga aaaccgcctc tatgattgaa caaaaagtca gtgtttatca
      181 gcagaagaaa ggccaggcag gcaaactgca ttgggacgaa gaagatattt tcttgattac
      241 ttatggggat caattttttg aagagcagca accaactttg cgtacattta agaaatttta
      301 tcaaaagtat ttggcagata catttagagt tgtacatttc ttgccatttt tcccatatac
      361 atctgatgat ggtttctccg ttgtaaatta tcaggcaatc aacgaccgtt tgggcacatg
      421 ggaagatata gaagaaatga gaagcgaaac acggttaatg ttcgactttg tctgcaatca
      481 tatgtctgcc gaaagtactt ggtttcagga atatttaaac ttgaatgata cgtacaaaga
      541 tttttttatt gatttgccac tggatacaga tacaagcagc gtgactagac caaggaccag
      601 cccgttactt acaacgttca ttgacaaaaa cgggaaagaa aaacacgtat ggacgacttt
      661 tagcgaagat caagtagact tgaattttaa aaatccagaa gttttattga aaatgatcga
      721 tattcttttg ttttacttag aaaaaggcgc ttcatttatt cgcttggacg ctgtgggatt
      781 tttatggaaa gaactaggaa cttcttgtat acacctgcca gaaacgcata aaatcatcca
      841 attattccgt gcaatcgctg atgaagcagc tccaggaacg gtattgatca ctgagacaaa
      901 tgtgccacac aaagataata tcagttattt tgggaatggc gagaatgaag cacagatggt
      961 ctatcagttt tcgctaccac ctttgaccct tcaagcgatt cgttcgggta atacaagtaa
     1021 gttgaaagaa tggttaaaaa ccatcgattt cgcatcgaaa aaaacaactt tttttaattt
     1081 tttagcctcc catgacggta tcggattgaa tccggtacga ggtattctgc cggaaaaaac
     1141 gatccttgaa ttagtggaac agctgaagaa agagggtgcc cgagtaaact acaagaaaaa
     1201 cccagatgga acgaccagtc catatgagat aaacgctacg tatctagatg cgttaagttc
     1261 tccaacagat acgacggaat ggagaagcaa acgcttttta gctgctcatt catttttggt
     1321 aacgttacca ggcgtgccgg caatttatat tcaaagtgtg ctgggaagtc gaaatgatta
     1381 caaaggcgta gaaaaaacag gagagaatcg aaaaatcaat cggcaaaaat ttttgttagc
     1441 agatattgag aaagagttgc agacagatcc attaagaaca gcagtatatg gtgaattgac
     1501 ccagatgcta tctattcgaa aaaaagaaaa agccttccat ccgaatagtt tgatttactt
     1561 actggactca gcagattctt ttatcagttt tattcgcgaa gcgaaggaag agaaagtctt
     1621 agttatccat aatttttcca atcaatcagc aggttatctt ttaccaaaag gagattacct
     1681 agacctgttt tctggacaaa ttatccattg cagtcaagaa cgaaagttga tattccagcc
     1741 gtatgaatat atgtggctga aaataataaa ataagaggtg gaaaaaaatg aaaaaaatta
     1801 ttgttactac ctgtctgcta gctactggat ttcttcttac tgcttgtggt gaaagtcaaa
     1861 gctctaacga atcacaacag acaacgattc aattcatgca ctcttctgtc gaacaggaac
     1921 gattaagtgt gatcaatgaa ctggttgctg attttgaaaa agaaaatcca gacatcaaaa
     1981 tcaagcaagt gccggtagaa gaatcagcgt tcaatacaaa aatcgttacg ttagctcgtt
     2041 ccggtaaatt accagaagta atggaagtca gtcaggattt tgctaaagtg atggataaag
     2101 atgaactgat tgatcagacg gcagtccaaa acgttatcga ggaagctgga gaagataatt
     2161 attatgacgg agccaaaaac cttgttcgtt cagaagatgg aaaaagctat attgctgcac
     2221 caattagcgg ctgggttcaa ggtatttggt atgacaaaga aaaattatca gaagctggtt
     2281 tttcagaacc agaaaattgg gatgatattt taaaaattgc tcaacatttc acagataaag
     2341 agaataaaca atatggaatt gctatgccta ctgctgaagg aacaatgagt gagcaagcgt
     2401 tctctcaatt tgctttgtca aatggagcaa atgttttgga tgatgaaggc gaggtaacga
     2461 tcgatacgga aaagatgaga gaagcattgt cgttttatca aaacttatcc caatacacga
     2521 tgccaggctc taatgatgtg acagaaatca aagatgcttt catgaatgga acagcaccaa
     2581 tggcaatcta ttcaacttac attttgcctt ctgtttatga agcggggaac tcagaaaata
     2641 tcggttttgc tatcccaaca caaaaagatc aagctgtcta tggcacagta tctggattga
     2701 cgatttcttc tggtctagac gagaaacaaa aagaggctgc tgaagcattc acagctttct
     2761 tatctcaacc gaaaaacatg gaaaagtggg tcttgatgtc tcctggtggt gctcaaccag
     2821 tgaataaaca agtagtcgaa ctggatggtt acaaagaaaa tgaagtcatc aagtcttttg
     2881 gcgagctgcc atctgagatt gctagtgctt ttgatgaagt acaagtcttt ggactagtcg
     2941 gggaaaagaa tttcacgaaa atgggagata tcacaagctc aggtgtgatc ggaaaagcag
     3001 tgaaccaagt aactgtcgga aacgaagatg tcgatcaagc tttagctgac gcacaaaaaa
     3061 atacgaaata acaagaagtt ctgccaatgg tgttcgaaaa aagtagatag ctattggcag
     3121 attatcaggg ggaggtttca atgaatcgtc caaggaaaca ttctgactta aaattagcca
     3181 tgggactttt agcaccaagc atgcttttat tagctgtttt agtgctgatt ccaatgcttt
     3241 ccaatattca gatcagtttt ttcgatatgc cgctgaatcc taactcagac gccgttttta
     3301 tcggattaag caattatctt gaaattttaa gtgatagtga attttatcgt tctttaggtt
     3361 taacagttgc ttacacattc ttagttgtag caggcagtac agctttagga ttagccgtag
     3421 ctattttgct taaccgcaag ttcagattta ggaaaacggt gcgttcactg attttattaa
     3481 gttatgttac gccatctatt tctttgattt ttgcttggaa atatatgttc aataatagtt
     3541 atggaatcgt caatgatctg ttagtagata ggttgcagct tttcaacgaa gcaccgctgt
     3601 ggttcgacca gccagttgct agcttcttta tggtcgtttt gtttgctatc tggagatact
     3661 atccgtatgc gtttatttca tttttggcta ttttgcaaac agtcgacaaa acgctttatg
     3721 aagcagctga aatggacggt gcaaatgctt ggcagaagtt caagatcgtg acactgccgg
     3781 caatcaaacc agtactagca actgtgattg ttttacgatc tatctggatg ttctatatgt
     3841 ttacagatgt ctatctactg acgaataaag tcaatatctt aggcgtttat ttgtatcaga
     3901 cagcctttgc gttcaatgat cttggaaaag cagcagctat atcgattcta ttgttcatca
     3961 ttattgcagc ggtcgtcttg ctctcacgaa agaaggtgga cttgaaatga aaaataaaaa
     4021 gagcaggaaa atcttatttt acgcgacgat tttgttgcta ttagctgtca atttgtttcc
     4081 atttttgatc atgctgatga catcattcaa ggattcacaa gaagcaattg caacgacaca
     4141 aactattttt ccgaaagaat ggacttttga acattatcag gatattttca atgctgagat
     4201 ttttccattc atcatgtatt tcaaaaatag tttagtgatc tccttgatag cctccgtaac
     4261 tgcagtagtg attgggatca gcggggcata cgctttgtca aaactacgct tcaaagctcg
     4321 ctcgaaaatc aatgctagct tttacaccgt ttatatgttt tccggtattt tgatcgttgt
     4381 tccattattt aaaatcattt ccggtctagg tttgtatgat accaatacag cactgattat
     4441 cacgatggtc gttcaaacac ttcctactgc gatattcatg ctaaaaagtt attttgacac
     4501 gattccgact gatattgaag aagcagcaat gatcgatggt cttagccgga tccagatcat
     4561 tattcggatc attataccat tggctatttc tggaatcatt tctgtcttcg tttattgctt
     4621 tatggtttcg tggaatgatt atttatttgc ttcgattttc ctgtcgacct ccgaaaaatt
     4681 tactttacca atcggtttga acacattatt cagtacacca gactacattt ggggacgaat
     4741 gatggccgct tcattggtga ccgctttacc agtcatcatt atgtatgcgt tatctgaacg
     4801 atttattaaa ggtgatttag tcgaaggcgg cgtaaaagga taaaaagaaa agaaagagga
     4861 aagaaaagat gaaaaaatta gttgcattag aaccaagaat agccggattc aaagattacg
     4921 aagaccgtga gatcgaaaag aacgaagtac gtatccgagt aattttcgct actccaaaac
     4981 caggaacaga aattgtcgac tttagaggat taagtccatt tatcgatgaa gaattttcag
     5041 aagaatggcg cctattcttg ccaagagaag aaggaagcca acgcggtgtg gtatttggcg
     5101 aatttcaatt aggaaatatg attgtcggta cgatcgaaga aacgggcagt ttggtgacag
     5161 agtatcaaaa aggagagtta gtctgcactt atggacccat tcgtgaaacg ctgattacaa
     5221 acggtgttga taattataaa ctacgtaaac ttgatcggtt ggatcaatgg aaaaatgcag
     5281 tatgttacga tcctgctcaa ttcgctttag gtggtatccg ggaagcacat gttcgagcag
     5341 gtgatgctgt agcaatcttc ggtttaggcg ctatcgggca aattgcgatt caattagcta
     5401 aaaaagcagg tgctactacc atcgttggga tcgatcccat agcgcagcgt agagaattag
     5461 cagaaaaaca cggtgccgat tacacgctag atcctatgaa agcagatgtc ggttttcgtc
     5521 taaaggaatt gacagataaa cgtggagtag attcagttat tgaaacgagt ggcagtgctg
     5581 ccgcattaca agcttcttta atagggatcg gctatggcgg gatcatttct tatgttgcct
     5641 ttgctaaacc ttttccagct gggtttaatc ttggtcgcga agcacatttc aataatgcaa
     5701 aaatcgtctt tgctcgagca tctagtgaac caaacccaga ttacccaaga tggaaccgta
     5761 aacgaatcga ggacacatgt tgggaattat tgatgaacgg ttacttggat tgtgaagaaa
     5821 tcattgatcc agttgtgcca tttgcagaaa gcgcagaagc ttacatgcgt tatgtagacc
     5881 agcacccaga agaaagtatc aaaatgggcg ttcaagtccg aggaggagaa caatgaaatt
     5941 cggtacgcaa gatcaagcat ttttcccaag agacatttta gaaaaattta gatttatcaa
     6001 agactgtgga tttgaagaat acgaaatcga cggtaagttg ttaatagaaa atatcgacac
     6061 agtcaaacag gcaatcaaag aaacagggat cccagtagtg acagcttgta acggctacga
     6121 tgggtggatc ggtgacttta tcgaagaacg gcgtcttaat ggtgtacgtc aaattgctga
     6181 gattttgcgt gcactaagcg aggttggagg caaaggaatc gttatcccag cagcttgggg
     6241 aatgttcact tatcgattac cgccgatggt ttcaccccga agcaaagaag gagactataa
     6301 agcagtcagc gaatcacttc gttatttaga taaagtagcc gaagaaacag gtacggttat
     6361 ttatttggag cctttgaacc gataccaaga ccatatgatc aatttgttag cggatgcgcg
     6421 tcattacatt gactctaatc aattgaaaca tgtgcagatc atcgctgatt tttatcatat
     6481 gaatattgaa gaagatgata tttcaaaagc attgactacg caaaaagagt atgtgggtca
     6541 tatccattta gcagataatc atcgctacca accaggatct ggcgcaattg attttactcg
     6601 ccattttcaa acgctacttc aaaatgagta tgaaggttat atggtatatg aatgtcgttt
     6661 gcgaggagaa aatgaagtga aaatttatca ggaatcattg caatacttga aaaactgtct
     6721 ggaaacagcg cgtcatttaa atgatttaga gaaagaatag agaaagaata acaataagta
     6781 aataagcaaa ggagagagaa ataaatgaag aaagtagcaa tcataggcgc tggacaagta
     6841 gcagaaaaag tacatgctgc ttattatcaa aaaagatcag atgtcttaga attggccgct
     6901 gtggttgatc cagtcagcca gcgagcagaa gacttttgcc gacgtacagg attttctcat
     6961 gcgtatacat cagttacaca aatgctgaat gagatttctc ccgatatcgt gagtgtctgt
     7021 acacccaatc gctttcactt cgaacatgta ttggctgccc tcgaagctaa tgcttcagtg
     7081 atgtgcgaga aaccaccagc tatgacagct gatcaagcaa gaaagatggc agagacagcc
     7141 gataaaaacg ggcagatttt ggcttatgga ttccagcatc gtttttctga cgaagcaaca
     7201 atgatcaaga atcatcttga tgttctcggg gatatttatt acgtagaagc gaatgcgctt
     7261 cgtcggtctg gtgttccagg ctggggaacg tttatcgaca aagaacttca aggaggcggg
     7321 cctttgatcg attatggcat tcacatgctt gatacagcga tgtatattac tggatttcct
     7381 aaagtaaaaa gcgttcgggc gtacagcttc caaaaaattg gtacgaaaaa aaattcaggg
     7441 aaattcggga aatgggatcc aagcaaattc acagtagaag atgcaatttt tggcacactg
     7501 gaattagaaa acggcaagat catccaactg aatacttcgt ttgctttgaa tatcagagaa
     7561 gacaaactga atgtggcttt ttgtggcgat caggcaggtg ctaatctgta tccaggcgaa
     7621 ctattcgaag atgtagatgg aaagctggtt tccttacaaa agaccacgcc agttaatgac
     7681 ccacatgaac taagtatggc tcattttgtg aatcgggcgc tcggtgacca gactcagatg
     7741 attgcagatg gctggcaagg atatgtcatc cagaatctgg tggaaaattt atatcgttcg
     7801 gcagaaacag gagaagttat taccttatga aagtagaaga aaaacagttt gatttagagt
     7861 atatcaataa gtatgcgagt cagatgacca ttggaaatgg attttttggt atcagaggaa
     7921 gccaggagga agactatcgg aatcaagtaa ggggaatgtt tgcagctggt gtatataacc
     7981 gacctttagg agcagaatca gccgagttag tgaatctccc agatgtcatc agatgggaaa
     8041 taaaaatcga cggtgaacat ttttctcttg agacgatgaa agttcttgca tacgagcggg
     8101 cactagattt agaaatggga gagcttaaac gctatgcgct cattgaaact gctcaaggaa
     8161 aaaaagtgga agtcattact tgtcgtgttg ctgatcaaaa taatctccat caggcagccc
     8221 tgaaagtggt catcaagccg ttgaccgaag actgccaagt agatgtgaaa acgggtatcg
     8281 atggacagca aaccaatttt ggtacacagc agttggttga aaaagaactg cgagtatttg
     8341 gtgacgattt gatcagtgca tcctaccaga caacagaaag taaaattgat attgggattg
     8401 cagctaaatt caataaagca ggtgtattcc aagcaaaaaa tcgccggatc accgcttcct
     8461 tttcagaaaa gatcagaaaa aatacgtcct ttacgctgga gaagatcatc gtgctctcta
     8521 cctctcttca gcaaacagac ccgtttaaga aaagtattgc ccaagcccag aaaagcgtta
     8581 gttatacaga aattttagag cagtcgatgt cttattggac agatttttgg catcagcatc
     8641 ggataacgat ccaaggcgat tcttctttcg atcagttagc gatggacttc gctgcttacc
     8701 atttacagat tatgactccc cgcaatgatc caagtgttag tgtaggggcg aagggactaa
     8761 ctggcgaagg ttataaaggg catgttttct gggatacgga aatttttatc ctgcctttct
     8821 ttttatatca gtttcctgaa gtagcaaaga atttgctgct gtaccgttat gatcgattag
     8881 aaggcgcacg gcaaaaagca agagattatc actatcaagg tgcacttttc ccatgggaaa
     8941 gcgcacacac tggtcaggaa gagacaccca aatttgctgc attgaatatc aaaaccggaa
     9001 caagacaaga agttgcttca gctgccgcag aacatcatat cagtgcagac cttgcttatg
     9061 ccgttcaaga gtatgtccaa gcaacagagg atcaatcgtt tttagaaaca aagggaaaaa
     9121 cgctcattca tgaaacagca gctttttggc tgagcagagc tgtaaaagtc gatgatcatt
     9181 tagagataca cagagtcatt ggtccagatg aatacacaga atatatcgac aataatgcat
     9241 atacgaacta tctggcgcat tataatgtgg ccttagctgt taaatatcaa gctggcgatc
     9301 cgcttttttt gaaagaatgt caaaattttt tagataagtt atatttgcca gtgcctaaca
     9361 aagaacagct gattccgcaa gatgacacgt atctcagtaa gccagttatt gatttgacca
     9421 cttacaaaga acgtcctggc agccagtcta ttttacttga ttatgcccgt agcgaagtaa
     9481 atgaactcca agtcagcaag caagcagatc ttgtcatgct gttatactta ttacctgagt
     9541 atttttctca accaatcgtt tctaagaatt tgcattacta tgaaaacaaa acaatccacg
     9601 actcatcgtt gagtaaagcg attcacgcca tagaggcttg ccgtatccaa gaaatggaat
     9661 gggcgtatca tttattccaa gaagcgtgcc tgattgattt aggaccagat ccgcattcat
     9721 cagatgacgg aattcatgct gcttcattag gagcactatg gctagcagtt atatttggtt
     9781 tttcaggtat tacagtaacg aatggaactc tttctatttc tccaagactt ccttcagcat
     9841 gggaaaaaat ttcttttcct tttatttgga aaaatcacag gttgctattt gttttgacaa
     9901 aaaaacaaat agtaataaaa aaagagacga aagaaacatt acctattttt gtcaatggag
     9961 accaatacca gctggaaaaa gaactgatct tggaacggag gggaaaataa tgcgtattct
    10021 gattgcgcca gattctttca aagaaagtct gacagcctta gaggcagcac aagccattca
    10081 atcaggtttt caagaagttt ttgttcaggc tgaatatgat ctgctaccta taggcgatgg
    10141 aggagaagga acggtttcat cattagcaga cagtttaagg ttgaagcggc aaacagcagt
    10201 cgttactaat gcatttggca aaaacaaaga aatccagtac gcttcagacg gtcaaactgc
    10261 gatttttgaa atggcagaaa tcgtcggatt gaaagatgtc ccactaaacc aacaacagcc
    10321 acttaggtta tccacgcgag gagtaggaga actgatacga caattgttgc aacaaggtgt
    10381 tcgacacatc atcatcggcg ttggcggaag tagtaccaac gatggcggca tcggcatggc
    10441 agaaggtcta ggttatgctt tttacgatca agataatcaa aaagtagaag cactgggaga
    10501 aaatctaggt aaaattgcta cagttgataa aacgaacgtg cctattgagc tgattaaagc
    10561 caaaatcact gtcattactg atgttactaa tcttttatgc ggaaaagaag gcgcttcttt
    10621 tgttttcgga ccacaaaaag gcttgaaaga aaatgaatta gcagtggctg accaagccat
    10681 gcatgatttt tatatgagat ttgcccctca agtaatcgag gcatcgggca gtagtgctgg
    10741 tggtggaatg ggtgcaggac tgttagcatt tgcttcaggg aaaatagtct ctggcatcga
    10801 ctacgtattg gatttactgg agttcgataa acgggtacag caagcagacc tcgtaatcgt
    10861 aggcgaagga cgtctagatg gacaaagtct aaaaggaaaa gcgccagttg ggattgctag
    10921 aagagtacca gagggcgttc ctgtgatagc tatttgcgga agtgttggta aaggaagcga
    10981 aaaagcagca gatgttggca tttcggccgt ttttccgatc cttcctgcag ctgtgccttt
    11041 acaagaagct ttacagcaag ccactgataa tcttcatcgc acagctaaaa acatcgcatc
    11101 tttacttcaa gtcgttcatt ttcaaggagg taatcgatga aaatcaaagc agcactattc
    11161 gatttagatg gcgttcttgt agacacagct cgttatcatt acgaagcgtg gctggtttta
    11221 gctaaccagc tgtccattcc attcactgag aaagaaaacg agcaactgaa aggaatatcc
    11281 agaaccgagt cactagagcg attgctgtca tttggaaaaa tggaacagaa attctcagaa
    11341 aaagagaaat cagcctttgc tgagcaaaaa aacaatctat atcttcaagc gattcaaaaa
    11401 atggatgaaa catccgtttt gcctggtgca atagctgttc ttgagtactt gaaaaaaaca
    11461 aacatcaaga tcggattggg atcagcaagc aaaaatgcac gcctcatttt agaaaaaacg
    11521 aatttgacaa gttattttga tgtccttatt gacggcacgc aagtatcaaa agcaaaacct
    11581 gatccagaag tttttctcaa aggagcacag caattgaatg taccgccaaa tgcatgcttg
    11641 gtcattgaag attcagaagc tggttgtcaa gcagctttgg ctggtaacat gcatgtattg
    11701 ggaataggtg aaaacataaa tcttccttct gctgaatatg tgattcctga tttaaccgtt
    11761 tttgatcaag tacgttcgtt ttggcattta tcagaagccg tatcacgtat ataatagttt
    11821 ccgttttttc tgtcacaagc aaaacgttat cctccttttg gaaatcaagg tatgatgaga
    11881 aaaaaggggg attttatatg acaaaaaaag cagttttcgc aggcggatgc ttttggtgta
    11941 tggtcaaacc atttgaagag cagccaggga tcatttctgt gacatccggt tatactggcg
    12001 ggcatgtacc aaatccaact tatgaacaag taacaacagg cacaacaggg catacagaag
    12061 cagtagagat cgaatatgat ccagaagtta tttcttatga tcagttggtg gaaatctatt
    12121 ggcagcaaac cgatccgact gacgcattag gacagttcgc tgatcgcggt gattcttatc
    12181 gtccagtgat ctactataat gatgaagaag aacgaaatat tgcagagcgt tcaaaacaag
    12241 cattgcaaga cagcgggcga ttcgataagc caatcgtgac aaaaatcgaa ccaagaagcg
    12301 aattttatcc tgcagaagat taccatcagg attattacaa gaaaaacaag ctccattaca
    12361 atctttatcg agaaggttct ggacgtgcag gtttccttcg tcgacattgg ggcgtcaaga
    12421 aataagaagc cgaaaaagct acttatggaa caccgtatat cagaggttgt aaaaccagca
    12481 ttttgagcta atactgaggg tcagctgatt gaacaccatt tagtggagag gttgtgaacc
    12541 tgtcgcctag ctccaagtca caaggatttg gtaagagaac actatatatc agaggttgtg
    12601 acaagtcgtt tgtcacaacc ttttttcaat acctaagatg aaggacaatc agcaacgcac
    12661 tgctcaaaaa aggaatgaaa ggcagctttt ccaacttttt tttatgacag ataaagtaaa
    12721 cagaaaaaac ggatattcca aacagactag caagaaacaa gatctgaagc aactcttcta
    12781 gagacaagcc accactccaa gaaagcatca acagagtatc gccagctccg agataggatt
    12841 gaccatagcg aaggcacaca ccgcctacga taaacgttaa cagcacactt tgccattgga
    12901 agacgccggc ctgcaaccaa aaaatccata aaattgccca agaacagaat agcgtgtgag
    12961 aatggaccat gaagtaaaat aaatccatca acgaaaatag caaagccgaa agcatccata
    13021 tgaacgtcga aaaatttctt gcggaagaaa aagtaaagaa ccaggcaaaa agaccaccac
    13081 ataaggcttc tgccaacaaa tagtagaagg gcaaccgaca cctgcaatag cggcagcgaa
    13141 atcgttggac gatgatcgag agaatcggaa tcaattcata ccaagaaagc gaacgacagc
    13201 atttgacaca atgagatcta ggataaataa tagagtgacc taacggaatc cgctgggcgg
    13261 ttaaacataa aaatgatccg atacagcaac caatgataaa ataaagtaac acgccatctc
    13321 ccccttttca aaaccaagat acgcaagctt aaactaaatt tttgtacata cataaaattt
    13381 gaggtgaaaa aatgaacgtt tcaatcggat tagatatcgg aaccacacaa accaaagcag
    13441 ttgctttcac agatgaagcc caagaagcag catccgctta ttttcgctac cctttgatcc
    13501 aagaaacaca agggatggca gaacaagatc aggaattgat ctttcaaggt gtctgcaccg
    13561 ttataaaaga agtaattaaa cagttgcctt cagacacaat cattcaattt gtctccttct
    13621 cctctgcgat gcatggcctg atattgttgg atgaagagaa aacaccgtta tctcgaatga
    13681 tcacttgggc agataaccga gcagcggacg aagcagaaaa actgaaatat accgtgcttg
    13741 gcaaagaatt ttatcggcag actgggacgc cgattcatcc aatgacacca ttgaacaaaa
    13801 tcaagtggct gcaaaacagc caacctgaat tattcaaaaa aactgcttat tttgtcggta
    13861 tcaaagacta tatcttttat agattgtttg gcgaactgat cagcgactac ggaacagctt
    13921 caggaactgg atattttgat atccatgaat tccaatgggc agaagaagtg cttgactact
    13981 tgaacattcg aatagagcaa ttgccgcaag tctttccttc cacctatcaa gtaacaggtc
    14041 taccatcaaa aacagcagca gaactaggtt tgccaaatga tatatcattt gttttgggag
    14101 gggcagacgg gccgctttct aatctagggt taggtgcttt tggtgatacg atcgccgctt
    14161 tgaccgtagg aacgagcgga gcacttcgtt ttattactac tcagcctcag ttccatccag
    14221 aaatggagac gttttgttat gttctggatc agaaacattg ggtagtggga ggagcgacta
    14281 gcaatggtgc tggcatcttt gattgggcag ggcatacatt tttacaggaa gtcgttcaat
    14341 ctgctaaaga aaaaggagaa aatccatatg atgcactgct ttcggctatc gcttttgtgc
    14401 cagcgggtgc caatggtctg atttttcatc cctatttact tggtgagcga gcaccgcttt
    14461 gggacgcaga tgcgtttggc agttttatcg gtttacagcg gcaacataca gaaaaagaaa
    14521 tgatgaaggc gattctagaa gggatctgtc tcaaccttta tcggatttta aaaggagttt
    14581 gccaggaaaa tcagctcaca gagatccgtg ctacaggcgg gtttgctcaa tctccgattt
    14641 ttcgtcagat gatggctgat gtactgggtt acccgcttgt tttccctact gtcacagaag
    14701 catccgcatt aggagctgtg attttaggct ggagaagcat cggaaagatc caaagtatca
    14761 gtgaagcaca ggaattgata gaattgaaag agcaagttgg agtacaagaa actgatcata
    14821 aaaaatatca gaaaatctat ccattatttg tttcgacaca aaagcattta gcacagactt
    14881 atcagcaatt tgctcaactg cgagaagaat tagctgattc tgaaaaatga ttggctttac
    14941 ggataaaaaa gcgaactgaa aatcgttctc aggtaaatga ttttgttgta cttttcattg
    15001 aaagtctact attattttaa gtgtaagaaa atgttaggag gcaagaaaga tgaaattcca
    15061 acaaaccaaa gaagtattaa atcaattagt tgcagatcta agccaaatgt ctgttgttgt
    15121 tcaccaaaca cattggtaca tgagaggacc aggtttcctt acattgcacc caatgatgga
    15181 tgagttcatg gatgatttga acgctcagtt agatgaagta tccgaacgct tgatcacatt
    15241 agacggttca ccttattcta cattgcgtga atttgcagat aacacaaaaa tccctgatga
    15301 aattggccgc tgggatcgta ctattccaga acgtttggaa acacttgttg caggctatcg
    15361 ttatctagca gatctttacc aaaaaggaat cgaagtttct ggagaagaag gcgacgatcc
    15421 aacacaagat atctttatcg aattcaaaac agccacagaa aaacgtatct ggatgatcca
    15481 agctcatttg ggcaaagcgc cagagatcga cgcgtaaaaa ctgttgttga acttgaccga
    15541 aaaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac gtcaaaatag
    15601 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
    15661 accaagaggt tgtaagcctg ccgttcagct ccgagtcaca agaaacaatt ttaaaaaaca
    15721 gcttcttatg ttttatggat attgtgactt gtgaccgagg agttggcagg agaacaccgt
    15781 ttattcaaaa taaggggttg tgacaagact tttgtcacag cctctttttg tttttcattt
    15841 tgacaggaaa cgaccctttt atataaaaga aagaaaagaa gtaaagtttc atataaattt
    15901 ttcaatcaag cagaaaaacg ttgacattga ctagattagc gggtaatatg ttaaatggta
    15961 ttgtttgccc cacaaagagc cccggaaact cgaagtgtat gtcaaacatc agaacttaaa
    16021 ttggaggaaa ataacgtgcg tacaacatat atggccaaac caggcgaagt agaacgtaaa
    16081 tggtatgtag tagacgcaac tgatgttcca ttgggacgtc tttcaacagt tgttgcatct
    16141 gtactacgtg gtaaaaataa accaactttc acacctcatt tggatactgg cgatttcgtc
    16201 atcgtcatca atgcagacaa agtgaaatta acaggtaaaa aagcgactga caaaatttat
    16261 taccgtcaca gcaactaccc aggaggattg aaatcaatca ctgctggcga attgcgtgct
    16321 aaaaattctc gtcgtttgat cgaaacttct gtaaaaggta tgcttcctaa aaacacttta
    16381 ggccgcaaac aatttacaaa attgaacgta tacggtggag cagaacatcc acatgcagca
    16441 caacaaccag aagttttaga catcacaaac ttaatctaat aggagggaat ttcattggca
    16501 caagttcaat atatcggcac aggccgtcgt aaaaacgcag ttgctcgcgt acgtctagta
    16561 ccaggaactg gcaaaattac tgtaaataaa aaagatgttg aagaatacat tccacatgct
    16621 gacttgcgtg aagtaatcaa tcaacctttc gctgttactg aaacaaaagg cgcatatgac
    16681 gtatttgtta acgtaaacgg cggaggatac gctggacaat caggagctat ccgtcatggt
    16741 atcgctcgtg cattgctaga agttgatcct gacttccgtt tagctcttaa acgtgctggc
    16801 ttacttactc gtgacgcacg tatggtcgaa cgtaaaaaac caggtcttaa gaaagcacgt
    16861 aaagcttcac aattctcaaa acgttaatat cctttatata tcaacgtttc tggcactttt
    16921 caattatttg gaaagtgctt cttttgtttt taagaacaca tttaagaaca catatcaatc
    16981 acacttattc aatcctctct gaaaagcttc aaaaacagcc ttatcaacta tatctggtgt
    17041 gttcacatat atctttgttg tagaaatatc tgagtgtgtt aaagcatttg aaatttgatt
    17101 catatctgct ccaccttgtc tagctatagt tgcataagta tgacggagtt tgtgtggaga
    17161 taaatgaact aagtcaggat gccttctttt aattgaattg attcgatagt ttaggtaatc
    17221 ggcatgaaca ggacaattta cttctccaga tggtttagta taagtaaaga gatactgatc
    17281 taacgtttgc ttaattttta atttcaacaa ttgatcagct tgtaccgatt tccattcact
    17341 tagcaatgtc attacaactt ctggtacttg aatgacggta tcttttcgac cttttgtaaa
    17401 cttttttctt tgatatttat ctaaagtatg tttaagacgg accgtttgtt tttcaaaatc
    17461 aatatatttc cattgcaaag cgtaagtctc actttttcta tcaccaagat acagggtaat
    17521 cataaaaagt gtgtaatcat gaaaagtaag taaatgatta ttaaaatcat catttactgc
    17581 ttctatccaa ttagtaagtt cttttgatga tagtgcttgc ttaccttctt gtattctttt
    17641 ttcttctaaa gcattttttt taggtgcagt gatactttga ataattttag tagttcgatt
    17701 aaattcgata taatttagta tttctgcaat atcgaacatg gaacgaacat atccttttat
    17761 gatttttata tttgcatatt ctttcgattt tttggttaat tcatctgata ttatctcagt
    17821 attgatattt agataattca ttgcgtattc accaaacata ggtaaaatat gcaaccgaaa
    17881 tatatctttt gtattactaa tggttatatc actaggaggc ctgtttgatc taattgtttg
    17941 acctaattca tatctaggaa gccatttaga ttcgtaaaat tttttaaacg ttatttttcc
    18001 ttttaattct aatgagtttg cattctcctc tctcaaaact ttttctattt tcttcttcat
    18061 atcattttcg gctttaatcg ccaattgcct atttggaaat gttttttgat acttttctga
    18121 tgcaattcct gttatttctt taaacctttt tggatatctc agattagttt tatatttccc
    18181 atttgaaagc tttgtaggca aaatcctcac tccttaaaaa aagtaaggca gtatatttca
    18241 ctaattttag tatatgcggc gagaagaaaa tttcaagtta ttttaaatgt attcataaag
    18301 tctttaatat cttctacgtc aaagcgaact acaccaccaa ctcttattac tgggaaatta
    18361 tatatacgaa tccacttatc tagagtatta ttatcgattc ctagaaatgc acaggtttgt
    18421 tttttattca ggtagactct ggattctata aaatcatctc ctaaaatagt agttatgaaa
    18481 tttttgaatt gctttgggta agcacatagg aatttcacct ctccccagtt tacggcgagc
    18541 cttacggaag tatcattatt tgctgctatt gtcatggcat tttgttttcc cagaacaaaa
    18601 agaaatctct aagtatttgt cgctaatcag gtcatggcgt acttgagttt ctagctcgaa
    18661 tagcagggat cgttattcaa aaattgtatt taattgtcaa tgatcaaaaa tagttttttt
    18721 cctattcact attaataacg a
//
LOCUS       DAZBTH010000046        18740 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000046 DAZBTH010000000
VERSION     DAZBTH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.046"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHGNW_001566"
     CDS             256..531
                     /locus_tag="ACHGNW_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925860.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACHGNW_001567"
     CDS             544..2616
                     /locus_tag="ACHGNW_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6925861.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACHGNW_001568"
     CDS             2784..4667
                     /locus_tag="ACHGNW_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6925862.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACHGNW_001569"
     CDS             4679..5626
                     /locus_tag="ACHGNW_001569"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN6925863.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACHGNW_001570"
     CDS             5646..6173
                     /locus_tag="ACHGNW_001570"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6925864.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACHGNW_001571"
     CDS             complement(6236..6889)
                     /locus_tag="ACHGNW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN6925865.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACHGNW_001572"
     CDS             7021..7863
                     /locus_tag="ACHGNW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6925866.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACHGNW_001573"
     CDS             8021..8917
                     /locus_tag="ACHGNW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6925867.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACHGNW_001574"
     CDS             8920..9633
                     /locus_tag="ACHGNW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN6925868.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACHGNW_001575"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACHGNW_001575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6925869.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACHGNW_001576"
     CDS             10168..10395
                     /locus_tag="ACHGNW_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN6925870.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACHGNW_001577"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACHGNW_001577"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6925871.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACHGNW_001578"
     CDS             complement(11181..11723)
                     /locus_tag="ACHGNW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6925872.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACHGNW_001579"
     CDS             complement(11960..12358)
                     /locus_tag="ACHGNW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6925873.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHGNW_001580"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHGNW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN6925874.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACHGNW_001581"
     CDS             13471..14238
                     /locus_tag="ACHGNW_001581"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN6925875.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACHGNW_001582"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACHGNW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN6925876.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACHGNW_001583"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACHGNW_001583"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN6925877.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHGNW_001584"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHGNW_001584"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN6925878.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAZBTH010000047        18736 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000047 DAZBTH010000000
VERSION     DAZBTH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.047"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1529
                     /locus_tag="ACHGNW_001585"
     CDS             102..1529
                     /locus_tag="ACHGNW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925879.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1621..1986)
                     /locus_tag="ACHGNW_001586"
     CDS             complement(1621..1986)
                     /locus_tag="ACHGNW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6925880.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1983..2348)
                     /locus_tag="ACHGNW_001587"
     CDS             complement(1983..2348)
                     /locus_tag="ACHGNW_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6925881.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2540..2788)
                     /locus_tag="ACHGNW_001588"
     CDS             complement(2540..2788)
                     /locus_tag="ACHGNW_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925882.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2787..2915
                     /locus_tag="ACHGNW_001589"
     CDS             2787..2915
                     /locus_tag="ACHGNW_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN6925883.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2972..4666
                     /locus_tag="ACHGNW_001590"
     CDS             2972..4666
                     /locus_tag="ACHGNW_001590"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN6925884.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4733..4939
                     /locus_tag="ACHGNW_001591"
     CDS             4733..4939
                     /locus_tag="ACHGNW_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925885.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5317..6450
                     /locus_tag="ACHGNW_001592"
     CDS             5317..6450
                     /locus_tag="ACHGNW_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN6925886.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6481..6801
                     /locus_tag="ACHGNW_001593"
     CDS             6481..6801
                     /locus_tag="ACHGNW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN6925887.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6830..7294)
                     /locus_tag="ACHGNW_001594"
     CDS             complement(6830..7294)
                     /locus_tag="ACHGNW_001594"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925888.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7426..8679)
                     /locus_tag="ACHGNW_001595"
     CDS             complement(7426..8679)
                     /locus_tag="ACHGNW_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6925889.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8708..9025)
                     /locus_tag="ACHGNW_001596"
     CDS             complement(8708..9025)
                     /locus_tag="ACHGNW_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6925890.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9051..9371)
                     /locus_tag="ACHGNW_001597"
     CDS             complement(9051..9371)
                     /locus_tag="ACHGNW_001597"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6925891.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9835..11250)
                     /locus_tag="ACHGNW_001598"
     CDS             complement(9835..11250)
                     /locus_tag="ACHGNW_001598"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6925892.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11267..12583)
                     /locus_tag="ACHGNW_001599"
     CDS             complement(11267..12583)
                     /locus_tag="ACHGNW_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6925893.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12733..13431)
                     /locus_tag="ACHGNW_001600"
     CDS             complement(12733..13431)
                     /locus_tag="ACHGNW_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN6925894.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13720..14709)
                     /locus_tag="ACHGNW_001601"
     CDS             complement(13720..14709)
                     /locus_tag="ACHGNW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6925895.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="ACHGNW_001602"
     CDS             complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="ACHGNW_001602"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6925896.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15797..17431)
                     /locus_tag="ACHGNW_001603"
     CDS             complement(15797..17431)
                     /locus_tag="ACHGNW_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN6925897.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17418..>18736)
                     /locus_tag="ACHGNW_001604"
     CDS             complement(17418..>18736)
                     /locus_tag="ACHGNW_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925898.1"
                     /translation="FSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNR
                     EKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAV
                     FLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLL
                     GLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILG
                     IQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIV
                     INEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYT
                     VASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPT
                     ALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         5916 a         3694 c         3138 g         5988 t
ORIGIN      
        1 catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat
       61 ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg
      121 agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca
      181 atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca
      241 ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt
      301 acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa
      361 attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc
      421 aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat
      481 tagcttactt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa
      541 tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg
      601 agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata
      661 gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt
      721 tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa
      781 aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt
      841 ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc
      901 attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata
      961 ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg
     1021 gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc
     1081 aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag
     1141 aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc
     1201 ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac
     1261 atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct
     1321 tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg
     1381 atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata
     1441 caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag
     1501 ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc
     1561 ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc
     1621 tcacttattt tggacatcta ggggtccgat ctctttattt aaaaaataag aaatgatggt
     1681 tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga
     1741 ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat
     1801 ctttttaata gcattgttct cgcttactcg gtcgctttgc acttttgtat ggacaaataa
     1861 atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa
     1921 gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt
     1981 cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa
     2041 taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc
     2101 ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata
     2161 taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc
     2221 ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata
     2281 ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag
     2341 cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa
     2401 agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc
     2461 tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg
     2521 tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg
     2581 ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct
     2641 tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc
     2701 ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag
     2761 aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt
     2821 taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg
     2881 acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg
     2941 gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg
     3001 tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg
     3061 ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg caccgaccga
     3121 cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat
     3181 tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag
     3241 tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga gagattttga
     3301 tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga
     3361 tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat
     3421 gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga
     3481 tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac
     3541 acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca
     3601 aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct
     3661 tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac
     3721 tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat
     3781 taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat
     3841 tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt
     3901 agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta
     3961 caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt
     4021 taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga
     4081 accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa
     4141 agatgtcaat atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa
     4201 tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa
     4261 acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa
     4321 agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat
     4381 aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag
     4441 cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt
     4501 cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc
     4561 tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa
     4621 agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga
     4681 aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt
     4741 gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4801 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
     4861 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4921 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
     4981 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
     5041 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
     5101 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
     5161 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
     5221 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
     5281 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
     5341 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
     5401 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
     5461 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
     5521 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
     5581 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
     5641 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
     5701 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
     5761 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     5821 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     5881 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     5941 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     6001 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     6061 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     6121 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     6181 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     6241 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     6301 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     6361 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     6421 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     6481 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     6541 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     6601 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     6661 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     6721 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     6781 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     6841 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     6901 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     6961 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     7021 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     7081 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     7141 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     7201 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     7261 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     7321 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     7381 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc
     7441 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     7501 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     7561 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     7621 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     7681 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     7741 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     7801 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     7861 aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt
     7921 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     7981 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     8041 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     8101 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     8161 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     8221 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     8281 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     8341 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     8401 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     8461 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     8521 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     8581 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     8641 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     8701 ctgtagatta cgcgttcaat cgacgataca gattaatgat ctcttttgct aaatctgtaa
     8761 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     8821 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     8881 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     8941 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     9001 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     9061 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     9121 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     9181 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     9241 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     9301 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     9361 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     9421 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     9481 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     9541 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     9601 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
     9661 tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc acaacctctc
     9721 aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc
     9781 tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt
     9841 atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc
     9901 agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg
     9961 attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc
    10021 gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc
    10081 gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt catattctct
    10141 tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat
    10201 ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt
    10261 cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca
    10321 ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac
    10381 tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt
    10441 ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac
    10501 tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg
    10561 agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg
    10621 gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca tcatccactt
    10681 ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac
    10741 gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat
    10801 ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc
    10861 caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc
    10921 gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt
    10981 ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat
    11041 ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg
    11101 gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc
    11161 ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag
    11221 aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat
    11281 tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac
    11341 aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga
    11401 tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg
    11461 caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg
    11521 gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag
    11581 caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa
    11641 tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca
    11701 tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg
    11761 ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca
    11821 cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac
    11881 cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt
    11941 ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag
    12001 cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct
    12061 tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa
    12121 ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat
    12181 tttcattcac taattcataa acagttggat taacagataa ataagacatc actgcgatga
    12241 taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa
    12301 cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta
    12361 attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca
    12421 ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg
    12481 cgttttttat caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa
    12541 cattggctaa tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt
    12601 gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta
    12661 ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca
    12721 tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt
    12781 tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc
    12841 ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt
    12901 tttattaaac aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt
    12961 tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt
    13021 gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt
    13081 cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc
    13141 aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt
    13201 ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt
    13261 cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc
    13321 ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata
    13381 tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac
    13441 ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga
    13501 ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac
    13561 cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac
    13621 aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt
    13681 ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat
    13741 tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa
    13801 ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca
    13861 tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt
    13921 gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg
    13981 tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag
    14041 cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc
    14101 tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt
    14161 tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat
    14221 atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg
    14281 tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg
    14341 gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc
    14401 ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa
    14461 atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt
    14521 attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg
    14581 attatggata ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa
    14641 tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc
    14701 aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt
    14761 tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt
    14821 gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt
    14881 tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg
    14941 taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt
    15001 tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac
    15061 gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc
    15121 aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag
    15181 cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc
    15241 cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca
    15301 tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg
    15361 catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa
    15421 agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta
    15481 gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca
    15541 atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa
    15601 tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact
    15661 ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt
    15721 ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta
    15781 taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg
    15841 cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa
    15901 tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct
    15961 ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga
    16021 ttacttggct cttctcatta gttactctat atatgataga actaccagtt gttttataag
    16081 gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct
    16141 ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg
    16201 cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga
    16261 aatttttttt actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa
    16321 atagttctct tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag
    16381 aagagaatat tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg
    16441 tattttgcag aagataccat ggcaccaaac tagttgtagc tagccaagtc aaacacgtac
    16501 cataataaat ctttttttct atgtttgtta attttttaaa taaaatcagg ggaatacata
    16561 aagataacag accaactagt cccattgagt aagtagttaa gtcgttgttc atagcttcag
    16621 tcagactatc gaacacatta gatgcatgag catataaatt tgtcacgcta ggtttgttga
    16681 ttttttggta gagcatttgt tcaaacatcg gataccaaaa agcactcgtc agaaccaatg
    16741 tcattcctgc cgcactaaat aattttttaa atatagcaat aaaatctgca ttcttgaaac
    16801 ctttagaaac caaacgaata aaaataaaaa tgataattaa tgctgtactc attaatgttg
    16861 atagtacatg gctgtacact agcaaactca tactcaatgc tagctttaca gacggatact
    16921 tttcttcttt tccagagatg atttgataag cataaaataa tacagggata acaagtgtta
    16981 ttgcgatagc ttcggcaatt gcggaacgat agtagatgtc tactgtccga taagcagaga
    17041 aaatatagag acaactaaat aaaaatgccg cttttttttg ttttaaaaac cgctcgccat
    17101 aatgataagc tacgagtgtt gtacacaagt tcaataacca aacgtaaata atatagctta
    17161 caatcaaatt ttttgtcatc caatataaca ttacagctgg aaaaaaagtc agaaagggat
    17221 aaaaataatt tacaccatat ccagtataat tgaatgtctc ataattaatc ggactggtaa
    17281 aaatagtcga taaccccttg attcgagata agtgaaaaga aagatctcca gtggtataaa
    17341 tactctgtgt atttttgaaa aagatgaaag aatatatact actaactata attaatccac
    17401 tcaatattac tactttttta tttattttca ttcttcttgt ttatctcctt tttagtcaca
    17461 tatcgagaat ctttcaactt attaggtgca aatcgtttaa aacttgaata caccactaaa
    17521 caataagtca gaatactgat ccataaagcc atgagagcag ttggagtata ttttgttgta
    17581 atttctatct tattttctcc agcatttaca tccacattca ctaagccatg ttttttataa
    17641 gagtcattta cgactttacc gtttactttt acctgtgtac ctaaataagc atatactggt
    17701 actgtcactt tttgcttttt gttgcttgcc actgtgtaag taatgttatt atctgcagct
    17761 ttatacttcc ctttttccca cgattctcct acttttactt catgtttttc taagtgaggc
    17821 ctttctttta tcgcttctgt tggggcgtaa tcgtataatc ctcccatatc tgatgttgta
    17881 taacgtggga gtgtttcttc attgataaca atatgaccgt gttttgattg gataactttt
    17941 gcgtaatttt cttttgctgc tatagacgtg acgagtagaa acaccacagt agcgccaatg
    18001 attaaccatg aatatttttt attcatagtc tttttcataa aaacaagcgt tatgatcatt
    18061 gaagaaaaaa agatttggat acctaagata cgccaaggaa actggagtaa gcttatcggt
    18121 gttttttgaa acaaaaacca tggaaataaa ttagttacgc atacccatgt agaaactacg
    18181 ccaacataga tgattttttc ttttttctcc atttctttca gtatgaataa aggaaaaatc
    18241 aacgcaacca atcctaataa ccccatagtg tatgttgtaa tatcgttatt taacgcttga
    18301 atgatactat ctgaaataga aagtgcttct agctgcaaat ctgttttgaa gggactgtgt
    18361 atttcttgat aattcatctg ctgcaacata ggataccaaa aatagctagt taatgcacaa
    18421 gtcaaaaaaa cagcctttac taaattcacg accacaaatt ttatatcgtt ttttatttgc
    18481 ttgcttaaaa acaaacgaac tataatcata caagcaatca atgcactaca caatagtgta
    18541 gttaagacat gagtatacac aagtagactt atactgacag ataaagcgat gacttttttt
    18601 tctctattga atatcaatcc ataagcataa tataaaacag ctgggataat cgtaagagcg
    18661 attgcttctg caattgcgga tcgataatag atatcaactg ttcgataagt tgcaaaagta
    18721 tataaacaac taaata
//
LOCUS       DAZBTH010000048        17845 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000048 DAZBTH010000000
VERSION     DAZBTH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.048"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1246)
                     /locus_tag="ACHGNW_001605"
     CDS             complement(167..1246)
                     /locus_tag="ACHGNW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6925899.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2805
                     /locus_tag="ACHGNW_001606"
     CDS             1354..2805
                     /locus_tag="ACHGNW_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6925900.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3154..3786
                     /locus_tag="ACHGNW_001607"
     CDS             3154..3786
                     /locus_tag="ACHGNW_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN6925901.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4374..5813
                     /locus_tag="ACHGNW_001608"
     CDS             4374..5813
                     /locus_tag="ACHGNW_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6925902.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6108..6326
                     /locus_tag="ACHGNW_001609"
                     /pseudo
     CDS             6108..6326
                     /locus_tag="ACHGNW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6575..6790
                     /locus_tag="ACHGNW_001610"
     CDS             6575..6790
                     /locus_tag="ACHGNW_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925903.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7007..7263)
                     /locus_tag="ACHGNW_001611"
                     /pseudo
     CDS             complement(7007..7263)
                     /locus_tag="ACHGNW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7450..8361)
                     /locus_tag="ACHGNW_001612"
     CDS             complement(7450..8361)
                     /locus_tag="ACHGNW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6925904.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8380..9384)
                     /locus_tag="ACHGNW_001613"
     CDS             complement(8380..9384)
                     /locus_tag="ACHGNW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN6925905.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9381..11504)
                     /locus_tag="ACHGNW_001614"
     CDS             complement(9381..11504)
                     /locus_tag="ACHGNW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6925906.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11509..13956)
                     /locus_tag="ACHGNW_001615"
     CDS             complement(11509..13956)
                     /locus_tag="ACHGNW_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6925907.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13943..14332)
                     /locus_tag="ACHGNW_001616"
     CDS             complement(13943..14332)
                     /locus_tag="ACHGNW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6925908.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14392..14895)
                     /locus_tag="ACHGNW_001617"
     CDS             complement(14392..14895)
                     /locus_tag="ACHGNW_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN6925909.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14908..15132)
                     /locus_tag="ACHGNW_001618"
     CDS             complement(14908..15132)
                     /locus_tag="ACHGNW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925910.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15236..17146)
                     /gene="ltrA"
                     /locus_tag="ACHGNW_001619"
     CDS             complement(15236..17146)
                     /gene="ltrA"
                     /locus_tag="ACHGNW_001619"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN6925911.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         5217 a         3593 c         3256 g         5779 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     1681 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     1741 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     1801 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     1861 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     1921 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     1981 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     2041 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     2101 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     2161 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     2221 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     2281 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     2341 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     2401 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     2461 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     2521 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     2581 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     2641 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     2701 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     2761 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     2821 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     2881 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     2941 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     3001 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     3061 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     3121 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     3181 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     3241 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     3301 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     3361 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     3421 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     3481 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     3541 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     3601 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     3661 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     3721 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     3781 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     3841 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     3901 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     3961 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     4021 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     4081 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     4141 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     4201 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     4261 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     4321 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     4381 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     4441 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     4501 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     4561 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     4621 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     4681 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     4741 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     4801 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     4861 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     4921 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     4981 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     5041 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     5101 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     5161 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     5221 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     5281 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     5341 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     5401 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     5461 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     5521 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     5581 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     5641 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     5701 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     5761 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     5821 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     5881 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     5941 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     6001 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     6061 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     6121 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     6181 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     6241 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     6301 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     6361 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     6421 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     6481 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     6541 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     6601 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     6661 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     6721 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     6781 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     6841 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     6901 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     6961 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     7021 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     7081 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     7141 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     7201 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     7261 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     7321 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     7381 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     7441 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     7501 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     7561 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     7621 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     7681 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     7741 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     7801 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     7861 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     7921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     7981 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     8041 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     8101 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     8161 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     8221 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     8281 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     8341 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     8401 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     8461 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     8521 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     8581 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     8641 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     8701 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     8761 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     8821 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     8881 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     8941 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     9001 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     9061 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     9121 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     9181 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     9241 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     9301 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     9361 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     9421 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     9481 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     9541 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     9601 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     9661 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     9721 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     9781 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     9841 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     9901 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     9961 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    10021 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    10081 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    10141 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    10201 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    10261 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    10321 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    10381 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    10441 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    10501 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    10561 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    10621 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    10681 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    10741 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    10801 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    10861 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    10921 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    10981 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    11041 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    11101 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    11161 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    11221 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    11281 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    11341 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    11401 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    11461 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    11521 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    11581 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    11641 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    11701 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    11761 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    11821 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    11881 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    11941 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    12001 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    12061 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    12121 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    12181 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    12241 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    12301 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    12361 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    12421 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    12481 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    12541 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    12601 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    12661 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    12721 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    12781 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    12841 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    12901 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    12961 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    13021 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    13081 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    13141 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    13201 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    13261 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    13321 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    13381 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    13441 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    13501 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    13561 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    13621 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    13681 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    13741 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    13801 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    13861 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    13921 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    13981 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    14041 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    14101 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    14161 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    14221 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    14281 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    14341 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    14401 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    14461 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    14521 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    14581 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    14641 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    14701 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    14761 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    14821 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    14881 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    14941 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    15001 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    15061 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    15121 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    15181 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    15241 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    15301 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    15361 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    15421 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    15481 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    15541 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    15601 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    15661 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    15721 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    15781 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    15841 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    15901 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    15961 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    16021 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    16081 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    16141 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    16201 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    16261 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    16321 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    16381 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    16441 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    16501 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    16561 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    16621 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    16681 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    16741 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    16801 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    16861 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    16921 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    16981 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    17041 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    17101 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    17161 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    17221 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    17281 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    17341 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    17401 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    17461 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    17521 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    17581 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    17641 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    17701 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    17761 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    17821 ttcttttatt agtgccttag ctttt
//
LOCUS       DAZBTH010000049        17225 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000049 DAZBTH010000000
VERSION     DAZBTH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.049"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHGNW_001620"
     CDS             1..369
                     /locus_tag="ACHGNW_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN6925912.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHGNW_001621"
     CDS             549..1490
                     /locus_tag="ACHGNW_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN6925913.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHGNW_001622"
     CDS             1559..2290
                     /locus_tag="ACHGNW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6925914.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHGNW_001623"
     CDS             2434..4614
                     /locus_tag="ACHGNW_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN6925915.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHGNW_001624"
     CDS             4634..5116
                     /locus_tag="ACHGNW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN6925916.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHGNW_001625"
     CDS             5132..5893
                     /locus_tag="ACHGNW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6925917.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHGNW_001626"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHGNW_001626"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN6925918.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHGNW_001627"
     CDS             7665..8150
                     /locus_tag="ACHGNW_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN6925919.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHGNW_001628"
     CDS             8143..8826
                     /locus_tag="ACHGNW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN6925920.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHGNW_001629"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHGNW_001629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN6925921.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHGNW_001630"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHGNW_001630"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN6925922.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHGNW_001631"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHGNW_001631"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN6925923.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHGNW_001632"
     CDS             13123..13455
                     /locus_tag="ACHGNW_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6925924.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHGNW_001633"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHGNW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN6925925.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHGNW_001634"
     CDS             14281..15654
                     /locus_tag="ACHGNW_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6925926.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHGNW_001635"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHGNW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN6925927.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHGNW_001636"
     CDS             16709..17149
                     /locus_tag="ACHGNW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN6925928.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3009 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggga
//
LOCUS       DAZBTH010000050        16647 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000050 DAZBTH010000000
VERSION     DAZBTH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.051"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHGNW_001637"
     CDS             complement(29..727)
                     /locus_tag="ACHGNW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN6925929.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHGNW_001638"
     CDS             complement(720..2072)
                     /locus_tag="ACHGNW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN6925930.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHGNW_001639"
     CDS             complement(2295..3695)
                     /locus_tag="ACHGNW_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6925931.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHGNW_001640"
     CDS             3856..4593
                     /locus_tag="ACHGNW_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN6925932.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHGNW_001641"
     CDS             4718..5197
                     /locus_tag="ACHGNW_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN6925933.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHGNW_001642"
     CDS             5194..5961
                     /locus_tag="ACHGNW_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN6925934.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHGNW_001643"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHGNW_001643"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6925935.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHGNW_001644"
     CDS             complement(6864..7466)
                     /locus_tag="ACHGNW_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6925936.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHGNW_001645"
     CDS             7572..7802
                     /locus_tag="ACHGNW_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925937.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHGNW_001646"
     CDS             7793..8041
                     /locus_tag="ACHGNW_001646"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6925938.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHGNW_001647"
     CDS             8147..8731
                     /locus_tag="ACHGNW_001647"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6925939.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHGNW_001648"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHGNW_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN6925940.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHGNW_001649"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHGNW_001649"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN6925941.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHGNW_001650"
     CDS             10632..10811
                     /locus_tag="ACHGNW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925942.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHGNW_001651"
     CDS             10833..11858
                     /locus_tag="ACHGNW_001651"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN6925943.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHGNW_001652"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHGNW_001652"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN6925944.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHGNW_001653"
     CDS             13430..14377
                     /locus_tag="ACHGNW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN6925945.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHGNW_001654"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHGNW_001654"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN6925946.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHGNW_001655"
     CDS             complement(15279..15521)
                     /locus_tag="ACHGNW_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925947.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHGNW_001656"
     tRNA            15642..15714
                     /locus_tag="ACHGNW_001656"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHGNW_001657"
     CDS             complement(15833..16471)
                     /locus_tag="ACHGNW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN6925948.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5674 a         2962 c         3338 g         4673 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactg
//
LOCUS       DAZBTH010000051        16549 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000051 DAZBTH010000000
VERSION     DAZBTH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.052"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..257)
                     /locus_tag="ACHGNW_001658"
     CDS             complement(<1..257)
                     /locus_tag="ACHGNW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925949.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="ACHGNW_001659"
     CDS             complement(273..923)
                     /locus_tag="ACHGNW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925950.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="ACHGNW_001660"
     CDS             complement(934..2106)
                     /locus_tag="ACHGNW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN6925951.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="ACHGNW_001661"
     CDS             complement(2125..2997)
                     /locus_tag="ACHGNW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6925952.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="ACHGNW_001662"
     CDS             complement(3019..3939)
                     /locus_tag="ACHGNW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925953.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="ACHGNW_001663"
     CDS             complement(3939..4208)
                     /locus_tag="ACHGNW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925954.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="ACHGNW_001664"
     CDS             complement(4205..4585)
                     /locus_tag="ACHGNW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFN6925955.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="ACHGNW_001665"
     CDS             complement(5052..6515)
                     /locus_tag="ACHGNW_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6925956.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="ACHGNW_001666"
     CDS             complement(6579..9596)
                     /locus_tag="ACHGNW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6925957.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="ACHGNW_001667"
     CDS             complement(9747..10586)
                     /locus_tag="ACHGNW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFN6925958.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="ACHGNW_001668"
     CDS             10731..10976
                     /locus_tag="ACHGNW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925959.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="ACHGNW_001669"
     CDS             11039..11461
                     /locus_tag="ACHGNW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925960.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="ACHGNW_001670"
     CDS             complement(11555..11872)
                     /locus_tag="ACHGNW_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925961.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="ACHGNW_001671"
     CDS             complement(11899..12105)
                     /locus_tag="ACHGNW_001671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925962.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="ACHGNW_001672"
     CDS             complement(12086..12268)
                     /locus_tag="ACHGNW_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925963.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="ACHGNW_001673"
     CDS             complement(12282..12632)
                     /locus_tag="ACHGNW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925964.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="ACHGNW_001674"
     CDS             complement(12658..12960)
                     /locus_tag="ACHGNW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925965.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="ACHGNW_001675"
     CDS             complement(12963..13196)
                     /locus_tag="ACHGNW_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925966.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14100..14630)
                     /locus_tag="ACHGNW_001676"
     CDS             complement(14100..14630)
                     /locus_tag="ACHGNW_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925967.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14627..15742)
                     /locus_tag="ACHGNW_001677"
     CDS             complement(14627..15742)
                     /locus_tag="ACHGNW_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6925968.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="ACHGNW_001678"
     CDS             complement(15748..16146)
                     /locus_tag="ACHGNW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6925969.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4392 a         3450 c         2577 g         6130 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatt
//
LOCUS       DAZBTH010000052        16340 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000052 DAZBTH010000000
VERSION     DAZBTH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.053"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACHGNW_001679"
     CDS             135..2606
                     /locus_tag="ACHGNW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6925970.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACHGNW_001680"
     CDS             complement(2709..4445)
                     /locus_tag="ACHGNW_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6925971.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACHGNW_001681"
     CDS             complement(4442..5146)
                     /locus_tag="ACHGNW_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6925972.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACHGNW_001682"
     CDS             5277..5780
                     /locus_tag="ACHGNW_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN6925973.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACHGNW_001683"
     CDS             5740..6078
                     /locus_tag="ACHGNW_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN6925974.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACHGNW_001684"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACHGNW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN6925975.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACHGNW_001685"
     CDS             7629..8207
                     /locus_tag="ACHGNW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN6925976.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACHGNW_001686"
     CDS             8211..8732
                     /locus_tag="ACHGNW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN6925977.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACHGNW_001687"
     CDS             complement(8811..9365)
                     /locus_tag="ACHGNW_001687"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN6925978.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHGNW_001688"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHGNW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN6925979.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACHGNW_001689"
     CDS             9905..10156
                     /locus_tag="ACHGNW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN6925980.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACHGNW_001690"
     CDS             10281..11072
                     /locus_tag="ACHGNW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6925981.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACHGNW_001691"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACHGNW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN6925982.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACHGNW_001692"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACHGNW_001692"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN6925983.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACHGNW_001693"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACHGNW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN6925984.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="ACHGNW_001694"
     CDS             complement(13129..14265)
                     /locus_tag="ACHGNW_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6925985.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="ACHGNW_001695"
     CDS             complement(14381..15295)
                     /locus_tag="ACHGNW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN6925986.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="ACHGNW_001696"
     CDS             complement(15386..15814)
                     /locus_tag="ACHGNW_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6925987.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="ACHGNW_001697"
     CDS             complement(15819..16193)
                     /locus_tag="ACHGNW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6925988.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5270 a         2946 c         3213 g         4911 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa
//
LOCUS       DAZBTH010000053        16314 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000053 DAZBTH010000000
VERSION     DAZBTH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.054"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHGNW_001698"
     CDS             complement(152..550)
                     /locus_tag="ACHGNW_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN6925989.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHGNW_001699"
     CDS             complement(547..1320)
                     /locus_tag="ACHGNW_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6925990.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHGNW_001700"
     CDS             complement(1317..2000)
                     /locus_tag="ACHGNW_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6925991.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHGNW_001701"
     CDS             complement(2167..3054)
                     /locus_tag="ACHGNW_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925992.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHGNW_001702"
     CDS             complement(3051..3920)
                     /locus_tag="ACHGNW_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6925993.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHGNW_001703"
     CDS             complement(4057..5343)
                     /locus_tag="ACHGNW_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6925994.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHGNW_001704"
     CDS             5616..6194
                     /locus_tag="ACHGNW_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6925995.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHGNW_001705"
     CDS             6194..7123
                     /locus_tag="ACHGNW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6925996.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHGNW_001706"
     CDS             7120..8856
                     /locus_tag="ACHGNW_001706"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6925997.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHGNW_001707"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHGNW_001707"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN6925998.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHGNW_001708"
     CDS             complement(9752..10261)
                     /locus_tag="ACHGNW_001708"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN6925999.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHGNW_001709"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHGNW_001709"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN6926000.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHGNW_001710"
     CDS             complement(11562..12653)
                     /locus_tag="ACHGNW_001710"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN6926001.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHGNW_001711"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHGNW_001711"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN6926002.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHGNW_001712"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHGNW_001712"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN6926003.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHGNW_001713"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHGNW_001713"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN6926004.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4703 a         3612 c         2903 g         5096 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagc
//
LOCUS       DAZBTH010000054        16225 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000054 DAZBTH010000000
VERSION     DAZBTH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.055"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACHGNW_001714"
     CDS             138..1475
                     /locus_tag="ACHGNW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN6926005.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACHGNW_001715"
     CDS             1523..3652
                     /locus_tag="ACHGNW_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN6926006.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACHGNW_001716"
     CDS             3627..4316
                     /locus_tag="ACHGNW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926007.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACHGNW_001717"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACHGNW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACHGNW_001718"
     CDS             complement(5546..5740)
                     /locus_tag="ACHGNW_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926008.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACHGNW_001719"
     CDS             complement(5790..6230)
                     /locus_tag="ACHGNW_001719"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN6926009.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACHGNW_001720"
     CDS             complement(6234..7079)
                     /locus_tag="ACHGNW_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN6926010.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACHGNW_001721"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACHGNW_001721"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN6926011.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQECATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACHGNW_001722"
     CDS             complement(8703..9650)
                     /locus_tag="ACHGNW_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6926012.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACHGNW_001723"
     CDS             complement(9873..10223)
                     /locus_tag="ACHGNW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6926013.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACHGNW_001724"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACHGNW_001724"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN6926014.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACHGNW_001725"
     CDS             11646..11966
                     /locus_tag="ACHGNW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6926015.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACHGNW_001726"
     CDS             11988..12452
                     /locus_tag="ACHGNW_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6926016.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHGNW_001727"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHGNW_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN6926017.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACHGNW_001728"
     CDS             complement(13301..14590)
                     /locus_tag="ACHGNW_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN6926018.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHGNW_001729"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHGNW_001729"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN6926019.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACHGNW_001730"
     CDS             15700..15825
                     /locus_tag="ACHGNW_001730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926020.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..>16225
                     /locus_tag="ACHGNW_001731"
     CDS             15822..>16225
                     /locus_tag="ACHGNW_001731"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN6926021.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
BASE COUNT         5134 a         2971 c         3296 g         4824 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aatgcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DAZBTH010000055        15951 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000055 DAZBTH010000000
VERSION     DAZBTH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.056"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..893
                     /locus_tag="ACHGNW_001732"
                     /pseudo
     CDS             226..893
                     /locus_tag="ACHGNW_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            complement(1093..1479)
                     /locus_tag="ACHGNW_001733"
     CDS             complement(1093..1479)
                     /locus_tag="ACHGNW_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926022.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHGNW_001734"
     CDS             complement(1451..2833)
                     /locus_tag="ACHGNW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6926023.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHGNW_001735"
     CDS             complement(2989..4176)
                     /locus_tag="ACHGNW_001735"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN6926024.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHGNW_001736"
     CDS             complement(4183..5214)
                     /locus_tag="ACHGNW_001736"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6926025.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACHGNW_001737"
     CDS             complement(5230..6684)
                     /locus_tag="ACHGNW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN6926026.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="ACHGNW_001738"
     CDS             6859..7764
                     /locus_tag="ACHGNW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN6926027.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHGNW_001739"
     CDS             7855..8532
                     /locus_tag="ACHGNW_001739"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6926028.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHGNW_001740"
     CDS             8529..8699
                     /locus_tag="ACHGNW_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926029.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHGNW_001741"
     CDS             8813..9709
                     /locus_tag="ACHGNW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6926030.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHGNW_001742"
     CDS             complement(9764..11134)
                     /locus_tag="ACHGNW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6926031.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHGNW_001743"
     CDS             11372..12118
                     /locus_tag="ACHGNW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6926032.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHGNW_001744"
     CDS             12115..12954
                     /locus_tag="ACHGNW_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6926033.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHGNW_001745"
     CDS             12955..13905
                     /locus_tag="ACHGNW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN6926034.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHGNW_001746"
     CDS             14046..14798
                     /locus_tag="ACHGNW_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926035.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15951
                     /gene="queG"
                     /locus_tag="ACHGNW_001747"
     CDS             14817..>15951
                     /gene="queG"
                     /locus_tag="ACHGNW_001747"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN6926036.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLL"
BASE COUNT         5168 a         3000 c         3215 g         4568 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaatgaa aaggtcaatc
      481 gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg cgtcatcaac atgacagagt
      541 tgacaaataa ccatatcgag atggagcagt tgcgagcaat cttaagacag caagaatgct
      601 tttctcttga aaatgccaaa catgttatcc tagagaatgc tggtcaaatg agtgtattga
      661 aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt agatgaaggg caaattcagc
      721 ataaagtgct tcagtccaat caattgactg aagcatggct tatggagaac ttgaagaaag
      781 aaggctatgc agatgtaaaa cagctgattt atgttgaatg gtcagaagaa aaagggctct
      841 acatactaaa taaagaagac gtcatgcatg aatcttaccg aatagatgga tagatagaca
      901 gaggttgtga aatcagcgcc ttggcttgtt actcaaaact aagcgacttt ttcacaacct
      961 ctgatatcta atatacgttg ttccagaagt tgcttcttcg aaaaataagc caaaattcca
     1021 caaaaattta agaagcaatt ttcgaaaaat cctttcttat tttttgaagt tgatcacttc
     1081 tgtcacaacc tctcatgtca caaaccattt ttttgtttcc tttaactcaa tataccgtac
     1141 gtcttcccac aatatcggct gatggtcaat cataaagtaa tcttcatagg cttcaccaat
     1201 gaagtgccct tcgacctcat ccaagagtct tccaaattca tcttttaaat tgagctgaat
     1261 gattactgct ttacgagata aaaaggcttc tgtcagtata ctatcgattt cttgcaatgt
     1321 catttgttgt ttaggaagag gctgtttgcg tgcttcttct tcattttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaaca actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgtcactg tttcaaatac tgatccagct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatacctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca attcctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agccggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa gtatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg c
//
LOCUS       DAZBTH010000056        15940 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000056 DAZBTH010000000
VERSION     DAZBTH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.057"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(376..798)
                     /locus_tag="ACHGNW_001748"
     CDS             complement(376..798)
                     /locus_tag="ACHGNW_001748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN6926037.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(812..2218)
                     /gene="atpD"
                     /locus_tag="ACHGNW_001749"
     CDS             complement(812..2218)
                     /gene="atpD"
                     /locus_tag="ACHGNW_001749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN6926038.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2241..3143)
                     /locus_tag="ACHGNW_001750"
     CDS             complement(2241..3143)
                     /locus_tag="ACHGNW_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN6926039.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3157..4713)
                     /gene="atpA"
                     /locus_tag="ACHGNW_001751"
     CDS             complement(3157..4713)
                     /gene="atpA"
                     /locus_tag="ACHGNW_001751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN6926040.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4736..5278)
                     /locus_tag="ACHGNW_001752"
     CDS             complement(4736..5278)
                     /locus_tag="ACHGNW_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN6926041.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5265..5789)
                     /gene="atpF"
                     /locus_tag="ACHGNW_001753"
     CDS             complement(5265..5789)
                     /gene="atpF"
                     /locus_tag="ACHGNW_001753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN6926042.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5880..6095)
                     /gene="atpE"
                     /locus_tag="ACHGNW_001754"
     CDS             complement(5880..6095)
                     /gene="atpE"
                     /locus_tag="ACHGNW_001754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN6926043.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6148..6867)
                     /gene="atpB"
                     /locus_tag="ACHGNW_001755"
     CDS             complement(6148..6867)
                     /gene="atpB"
                     /locus_tag="ACHGNW_001755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN6926044.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7169..7633)
                     /gene="smpB"
                     /locus_tag="ACHGNW_001756"
     CDS             complement(7169..7633)
                     /gene="smpB"
                     /locus_tag="ACHGNW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN6926045.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7655..10012)
                     /gene="rnr"
                     /locus_tag="ACHGNW_001757"
     CDS             complement(7655..10012)
                     /gene="rnr"
                     /locus_tag="ACHGNW_001757"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN6926046.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10015..10770)
                     /locus_tag="ACHGNW_001758"
     CDS             complement(10015..10770)
                     /locus_tag="ACHGNW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6926047.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10873..11109)
                     /gene="secG"
                     /locus_tag="ACHGNW_001759"
     CDS             complement(10873..11109)
                     /gene="secG"
                     /locus_tag="ACHGNW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN6926048.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11227..11505)
                     /locus_tag="ACHGNW_001760"
     CDS             complement(11227..11505)
                     /locus_tag="ACHGNW_001760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926049.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11618..12586)
                     /locus_tag="ACHGNW_001761"
     CDS             complement(11618..12586)
                     /locus_tag="ACHGNW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926050.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12801..12845
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12869..13843
                     /locus_tag="ACHGNW_001762"
     CDS             12869..13843
                     /locus_tag="ACHGNW_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFN6926051.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13848..14423
                     /locus_tag="ACHGNW_001763"
     CDS             13848..14423
                     /locus_tag="ACHGNW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN6926052.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14490..15629)
                     /locus_tag="ACHGNW_001764"
     CDS             complement(14490..15629)
                     /locus_tag="ACHGNW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN6926053.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4353 a         3440 c         2853 g         5294 t
ORIGIN      
        1 tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta ttgacaaaga
       61 gcccaaaagt cttgtcgcag tcccttattc caaataaacg gtgttcaatc atctgaccct
      121 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttccatttgt ttctctaata
      181 ctcaggacaa aacgctgatt tcacaacctc cgatatacgg tgttccagaa gtagcttctt
      241 cgaaaaataa gctgagattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
      301 tgtcttgaag ctgaccactt ctgtcataac ctctttttac tgttcttaaa cacggataaa
      361 catccgcatc tcattttatg agtgcttaga tactttgata cgattgattg ctcggtgcaa
      421 cgcaacagtt gcccgtttca attcgttcgt atcttgtttt gcttttgctt cttcgatttg
      481 gcgttctgcg cgttgttttg cacgttccgc acgagataca tcgatatcac gttcacgttc
      541 cgcactatca gcaacgattg aaacaacatt atcacgaact tccatgattc cgccattaac
      601 agcgatccaa tcgacgtggg tatctgaatc tgttcgtttt acacgaactt catcaatcgt
      661 caatggaacg atgattggtg catgtttagg caaaatccca agctcaccag ctgtcgtacg
      721 tgcaacgacg atagctgcat ggtgatcata gaccaaaccg gcaggagtta ccacatttac
      781 tgttaaatat tgatccatgg ggcacccctt tctagtagcc tagtgttttc gctttttcta
      841 cgacgtcttc gattcggcca acacttcgga aagcttcttc aggaagatca tcgtatttgc
      901 cttccaagat ttcgcggaat cctttcactg tttcttctac agggacatat gaacctggaa
      961 gaccagtaaa ttgttcagcc acgttaaagt tttgtgacaa gaagaattgg actctgcggg
     1021 cacgagaaac taacactttt tcttgatcgg ataattcatc catacctaag atagcaatga
     1081 tatcttgtaa ttcacgatat cgttgcaata cgtgttggac ttctgttgct actttataat
     1141 gttcttctcc cacgatttca ggtgctaaag cacttgaaga agaagccaat ggatctaccg
     1201 ctggataaat cccttgttct gttaatttac gttccaagtt ggttgtcgca tccaaatggg
     1261 cgaatgctgt tgccggcgcc gggtcagtgt agtcatcggc tggcacatag attgcttgga
     1321 tcgatgtgat cgaacctttt ttcgtagatg tgatacgttc ttgcaattga cccatttctg
     1381 ttgccaatgt tggttgataa ccaaccgcag aaggcatccg tcctaacaag gctgatactt
     1441 cagatccagc ttgagtgaaa cggaaaatgt tgtcgataaa caacagtaca tcttgacctt
     1501 ctacatcacg gaaatattcc gcaatcgtca aaccagtcaa agcaacacgc atacgtgcac
     1561 ctggtggttc gttcatttgt ccgaacacca tggctgtttt ttcgataact cctgaatctt
     1621 tcatttcata atacaagtca ttaccttcac gagtacgttc acctacaccg gtaaatacag
     1681 aaataccacc atgttcttgg gcgatattat ggatcagttc ttggatcagt acggttttac
     1741 caacaccggc accaccgaat agtccgactt tcccaccttt taaatatggg gcaagcaaat
     1801 cgataacttt gatccctgtt tccaaaattt ctgtacttgt acttaattcg tcaaaggctg
     1861 gtgctttttt atgaatttcg cttctttccg catcttctgg gaaaggtgtt tctaaatcga
     1921 tcgtatctcc tagtacgttg aatacgcgac ctaatgtttc tttacctaca ggaacagaga
     1981 ttgctttgcc tgtatcaatg acttccattc ctctttgtaa accatctgtt gattccatcg
     2041 caatggtccg gatcactccg tcccctaatt ctaacgctgc ttcaagaaca acttttgatt
     2101 tattttcgtc atttttataa acaactaatg cgttgttgat atctggtaag gattggtcta
     2161 aagaaaattc cacgtcgaca acgggaccga ttacttgaac aatcttgcct gaactcatct
     2221 tttttcctcc aatctcaata ctattctagt gccgaagctc cggcaacaat ttcggtaatt
     2281 tcttgggtaa tagccccttg tcttgcacgg ttgtaagaaa ccgtcagctc atcaatgata
     2341 ttagcagcat tatcagttgc tgttttcatt gctgtcatac cagcagcatg ttctgctgtt
     2401 ttggcgtcaa cgatcgcacc gtagatcaaa ctttcagcat actgaggcaa taactgagac
     2461 aagatttctt cttttgaagg ttcaaaaata tattcttgtt cgaaagtagt tgcttcttct
     2521 ggatctaagt ctgagattgg cagcattttt tctacacgaa actgactagt tagcgaattg
     2581 atatgatggt tataacatac gtataattca tcgaatactt cgttttggta catagttgtt
     2641 gccatattta cgattttgcg gacttcatca aagcttggct gatcagatag attgcgcagc
     2701 tcatatgcta gattgatacc gcgtgcttta aagaagtctg caccagttcc gccaattgcg
     2761 atcatcacgt aatcatcggg tgattggtgg tcttcttcta aaatagacat cgtttgtttc
     2821 aatatcgagc tgttatatcc accgactaaa ccgccatctg cagtgatgac gatataaccc
     2881 gttttcttaa caggacggct gagcaacatg ctgttgtaat tgatatcccc tctaggattt
     2941 tctgaggcaa tatcatttaa ctgagtagca gtcaaatgcg taacgatttc tcttactttt
     3001 gttgcataaa cctgaaactt ctgtgaagaa gcttcagatt ttgtcaactt agaagcagac
     3061 accatctgca ttgcccgcgt gatttggctc gtttttttcg ttgaagcgat acgtgtcttg
     3121 atttcattta atgaagcacc cattcatctc acctcactat gcattttgaa tggatttcaa
     3181 tttatcttct gtagaggaat tatttccttt tgtagctaag aacatgtctt tatattcctg
     3241 aatcgcactg ttcaacgctt cttcttctgg aagatctttt gttgtacgga ttgtttcaaa
     3301 gatatctgaa tgattggtat ccagatattc gaataattca tgttcgaagt ctaaaatact
     3361 gtctacaggg atactatcta agaatccatg tgtcaaagca tacaaaatca ctacttgttt
     3421 ttcaacagct agcggtgcat gcaatttttg tttcaagatt tctactgtac ggcgtccacg
     3481 atttagtttt gcttgtgttg ctgcatccaa gtcagaaccg aattgtgtaa atgcttctaa
     3541 ttcacggtaa cttgctaagt ctagacgtaa agtcccagca acttttttca ttgctttgat
     3601 ttgcgcagaa ccccctacac gagatacaga tagtccggca tcaaccgctg gacgagtacc
     3661 tgcgtagaac aagtcgcttt ctaagaagat ttgtccatca gtgatcgaaa taacgtttgt
     3721 tgggatataa gcagagatat ctccagcttg tgtttcaaca aatggtaagg cagtcattga
     3781 accgccacct aaatcatcac ttaattttgc tgcacgttcc aataagcgtg agtgtaagta
     3841 gaaaacatcc cctggataag cttcacgacc tggtggacgg cgaagcagca aggaaagttc
     3901 acgataagca acagcttgtt ttgaaagatc atcaaaaatg atcaagacat gtttcccgtt
     3961 gtacatgaac tcttcaccca ttgcagtacc agcatatggt gcgatataaa gcaacggcgc
     4021 tggttgagac gcaccggcat tcacaacgat tgtgtaatcc attgcgccat attttttcaa
     4081 tgtttcaact tgtgtacgaa ctgtagaatc tttttgtccg attgctacat agatacagat
     4141 catatcttga cctttttggt tgatgatcgt atcgatcgca atggaagttt tccctgtttt
     4201 acggtcaccg ataactaatt cacgttggcc gcgtccgatt ggtacaagag cgtcgattgc
     4261 ttttagacct gtttgcattg gttcgttaac agatttacgt tgcataacgc ctggtgccat
     4321 tgcttctact ggacgagctt tgtcagtaac gatttcacct agtccgtcaa ttggctgacc
     4381 tagcggatta actacccgtc caatcaaggc ctctccaact ggaacttcca tgatctttcc
     4441 tgttcgtttt actttatctc cttcacgaat ggactcgaaa tcgccaagga taatgatacc
     4501 tacatcattt gattctaagt tttgcgccat tccgtatgag ccatttgaaa attcaagcaa
     4561 ctcaccactc atcgcgtttt ctaaaccatg tgcacgagcg atcccgtcac ctacataagt
     4621 tacggtaccg atttcttcga ctgaaagcac gttttgataa ttttcaattt gttctttaat
     4681 caaggcacta atttcttctg ctttgatggc cattcgattc acctacctct ttgtactatc
     4741 tatttaattg attacgcata ttttctaatt gcgtgcgaat gcttccgtcg attactcgat
     4801 ggttagcttc aacaatcgcg ccaccaataa tagaagaatc aacgatttgt tttaattcga
     4861 ctgattgata gttcattgtt tttgcaacat tcttttctaa ttgtattcgt tgttcgtctg
     4921 aaagtggaac tgctgttgtg acacttccca acagcaaacc ttgatgttca tcatatcgac
     4981 gttcatattc ttcgatcatg aacaaaagat catccattcg gttatacgca taaacaactt
     5041 ctaaaaagtt tttaacgatt ccatcatagc cactaacaag cttgtccatg atttctcgtt
     5101 tttcatgagg ttctagacga acatcactta acatattacc taatccaggt acttgttcat
     5161 agatttgacg taagcttacg agatcttgat aaatttcttc tgctttattt gaatcgatcg
     5221 ccagttcaaa taaagctttc ccgtaacgtc ttcctactgt atatttatct agtttcattt
     5281 gaagaaccta gaccttcgat atattgattg attaatgatt cgtgcatttc tggagataat
     5341 tctttgttca agattttttc cgcgatttga agagatagtt ctgcaacatc gtcttttaca
     5401 gagttcagcg ctgaatcacg ttctacagtg atatcagctt gggctttact ttttagacga
     5461 gctgcttctt cttgagcatc cttcaaaata ttttggcggc ttaattctcc actttctttc
     5521 gcatttttga tgatatcagc tgcatcagaa cgagaggcta acaattgttg ttcgcgttct
     5581 tgttccattt tcgctgagtt gatgcgagat tgttctgcag aatccaaatc attggcgatc
     5641 ttgtcttcac gttttttcaa aatatcgctg attggtcccc aagcaaaatg cttcaagaga
     5701 gccagtagta tcaaaaatga gccgctgaca acgatgacat ttcctaacat cgcattgcca
     5761 acttctgcaa ttgccaattg attcagcata ggcgagcctt cctttctttg aagtacttct
     5821 agttttcatg taaaaaagca gtaacaaacc aaattgtttc tgccttaata ctgattcaat
     5881 taaacaccaa aaactagaat caaggcgaca actaccccta agataggaac agcttcgact
     5941 agagctaccc cgataaacat tgttgtacgg aattgaccag acatttcagg ttggcgagcc
     6001 attgattcga ttgttttaga gataacttga ccgttaccat aaccggctcc gattgctgct
     6061 cccatgattg cgattgctgc tgcgatataa ttcataattg ttttcctcct aaattagata
     6121 aatagtagtt tgtttttaaa acgaatatta ttcttccact tcaatcttat ggctcatata
     6181 aaccatagat aaagtaacga agacaaatgc ttggatgctt ccgataaaca aggagaatgc
     6241 gatccagacc atctctaatg gaattgctaa cggcaatgag aaccagccta agcttattac
     6301 catttttgca atcaaggtta gcaaaacttc ccccgcataa atattcccat aaagacgtaa
     6361 agcaagtgtt aataagtttg taaattcttc aatcagtttc atcggaaaca taaaactata
     6421 tggtttcaag aacgaattta cgaaatatcc tttgaagcct aactttttca ccccaaataa
     6481 gtgagtaagt aagatcatta taaatgccaa agttaaagta acaaacgggt ctgctgtcgg
     6541 gcttttccaa agtgttgttt cgttaggtaa aacgattttt gttactaaac cgatgttgtt
     6601 tgcaacaaaa acaaataaaa acaatgtaaa tgccaacaaa tggaaattgc tcaattcttt
     6661 tctaggcaga ttatctgtta cgattcctcg actaaaatca atgacccatt ctatcgcatt
     6721 ttgtttgcct gttggtttca acttaagatt tcttgtaaaa aagtaagcta agaagaaaac
     6781 aatcaaacag gtcagtaaca ccattataca aactgtacca tcaaaccaaa ttggtccaat
     6841 gtggaacgtt agcgagcgtt catccaatcc atttcacctc ttttccccga aattcacata
     6901 aaatcaattc atgtgcgtat ctggtttttt ccaatcggtt tctaaccaca cggtatcata
     6961 ccaccatgaa acagaaaatt caaaacattt tcatgaaaat tagacacttt tttatttcag
     7021 tcaaaaacga agcgaatttt tctttataca cggtgctcct gatctaagaa aaaacctatg
     7081 ataatcatgt tttatcagat taaatgctaa caaattttat cattaagtaa aagcaaaagc
     7141 accgaatatg cgaaaaagga taatgaaatt atctagagaa attttttaat gttcgatcaa
     7201 tttgtcgatc gatatctttt cgtttcaaat cttcccgttt atcataagat tttttccctt
     7261 tcgccagacc aatcaatacc ttggcatagc cgttacgaat atagactttt aaaggaacaa
     7321 cagtgattcc agtatttttc aattcatttt caagccggat gatttgtttt ttgtgtaata
     7381 ataactttct cgtgcgcaac ggatcatgat taaaaatatt tccttgttca taaggactga
     7441 tatgaacatt atgcaagaaa gcttctccgt tgcggacgcg aataaatcca tcttttagat
     7501 tgatccggct gtttcgtatc gacttgatct cggttccttg caagaccatc cctgcttcca
     7561 tcgtgtcgat gatcgaataa tcatggcgag ctttcttgtt ttgtgcaatt aattttccct
     7621 cgccttttgg catagctgtt tcctccaact cattttattt tttctttttc ttctttttaa
     7681 cacctttgta aaatggcttc ttcccttttt tcttaccgaa cgtttgctta ctatcgctat
     7741 atcgtttgtt cttcttgcca tttctttctg ggcgttttcc tgcttgtttt ccttcgcgtt
     7801 ttcctgattt tccatttcgt gtttttggcc gttccaacgc tgtaacctct tctgcggaaa
     7861 caagttcaaa atctactgct cgttcatctg gatcagcttt gactactttt actcgcactt
     7921 tctgtccgat tttaaaagtc agaccagttc tttcacctac aagagctaaa tgattttcaa
     7981 caaaatggaa gtagtcctgt ttcaattcat tgatatggat caatccctct actgtattcg
     8041 gcagttcgat aaacaggccg aatttcacaa cagagctgat gatcccatcg aattcttccc
     8101 ccactttgtc catcatgaat tctgctttct tcatggaatc tacttcacgt tctgtttcta
     8161 ctgcacgacg ttccatctta gaactatgat ctgcaatttc aggaagttcc gtagcccacg
     8221 aactttgatt gctttcactt ttatcttcac tgtaagaacg gatcagacga tggacgatta
     8281 gatcgggata gcgtcgaata ggagatgtaa agtgtgtgta gtaatctgcc gctaagccat
     8341 aatgcccaaa attgtcttct gaatagcgtg cttgttgcat gcttcttagc aacatagtat
     8401 tgataactgc agcttctggt ttgtcttcta ctccttcgat cacatgctgt aaatcttttg
     8461 gtgtgatcgt tcctttcgtt cctcggacca gaatgcccaa agctgaagca aaatcaaaga
     8521 agcgctgcat tttttcttct tttggctgtt catggattcg gtaaataaac ggcagattta
     8581 atttattata atgttcagct accgtttcat tggccgcaag catgaaagat tcgatcaagc
     8641 gttcaccaat tcctctgctt cttagctgga tatcatttgg gtgtccttca gcatcaacaa
     8701 ggatttttgc ttcgcgatcc tcaaaagaaa tcgcgccacg tttgattcgc atcgtttcaa
     8761 ggattcgatg caattcaccc atttcttgga acataggtac gagctgagca tagcgttgca
     8821 tcgtttcttc gtcttgatct tctaagattt gattgacagc tgtgtaggtc atccgttctg
     8881 ttgtttggat cacacttggg aaaatatcat gagagactac ttgtccattt ggatcaatct
     8941 ccatttcgca actcatggtc agccttggaa catggggatt cagtgaacag ataccattcg
     9001 ataaacgttg cgggatcatc ggaatcactc gatcagtcaa atatacactt gtccctcttt
     9061 catatgcttc tctgtccaat tgactgcctt gtgtcacata gtaagaaaca tctgcaatgt
     9121 gtactccaag aaaataatta ccgttatcta attttttaac cgtaactgca tcatctagat
     9181 cttttgcatc ttctccatca atcgtaacaa tgagctgatt tcttaaatct ctacgtccag
     9241 ttatatcctt ttcgctgatt tgatctggaa cttcatctgc ttcagacaat acatcatcag
     9301 gaaatttcgt agggatacca ttagccacga caattgagag gatatccatg ccagggtcat
     9361 tcttatgacc aatcacttgc ttgattatcc cttctaaact agtcgcatac cctttttcag
     9421 ggtaatgggt cagttcgatc agcacaatac ttccgtcaac tggacgaact ccctctgctg
     9481 ctgcatatac tttaaattgg ttgagctttc tatctttcgg gataacaacg ccgtaaagat
     9541 ccgtttcttg tatttcttct tctgaataag caacgaattc ccctgctaac tgagtagtcg
     9601 cccggtctcg aatctcgact actttcccct ctgctccgcg atccatatct gtatttgctc
     9661 gcttcatgat atcgattgct acggtgtctc catccattgc aaaaccagtc gcttcttttg
     9721 ggatatagat gtcatcttct tcgggatcaa tcgttacaaa tccaaatcct cgttcatttg
     9781 ctcgaaatgt tccttcgatc aagattggtt ttaaaggaag tttcactttt ccttttttat
     9841 tgaaaactat actctgttct ctttccattt gagcaatcgt ttgtactagt aatttaaaat
     9901 catcgctttt ttggaaccct aaaccttcag cgatttcttc catagaaaaa ctgcgtttgc
     9961 gactgctttc catgaaaaag agaattttct cttttatcgt ttcttttgtc atatttattc
    10021 ctcattccaa ggtaattttt ctaaaaattc caagacgtct ttttctaatt cgcgtcttgc
    10081 tgtaccaact gtaatcacat gaccactttc aggataccat cgaagtgtca ctcgctgacg
    10141 gctcaacttg cgtgcagttt caaatacgcc attagggtcg atcatttcat ctttcccagc
    10201 ttgagctaaa aatatcggct gttggatatc tgacaaccta ctttctacga ttgctgcctg
    10261 atcttgtata tccattaatt gttgttcagc taacgagcgg taagcaactg ctttttcatt
    10321 gatttctttc tctgatttac ctgctgtctt cagcacttga cgtacgtatt tttcaaagtt
    10381 ttcaggcacg tttgttttta caggagacaa aggcgaacag aaaaaaccgc cgccaatcac
    10441 tgattcttct tctagtgctc gtacagcaaa gatgccgccc attgataatc ctaatacggc
    10501 aatatcagaa aactgctcag actgtaaaaa atggatggct tttttagtgt ctgcccacca
    10561 gctttctgct ttttcttgca aaatatcata tggctccatt gttccgtgtc ctttaaaaag
    10621 cggcgcataa actgtataat ctgctttttc caaaaatcgt gcgagcattc gaacatcgtt
    10681 tggacttcct gagtaagcgt gaagcagcaa aaccgctcgc tttccatgtt ttgcataaag
    10741 gggttttggt aattgtctca ctcgtctcac ctctttcgcc tcattttacc atagtttctt
    10801 tcataacttc ttcttaagcg ttctctttgc aataaaaaaa gccgcccgcc gttttgcgag
    10861 aggcttattc aactacttcg aagataaata tgctaggaca aataataaga tcatccagat
    10921 agctcccata actgcagttg atcgctgcat gacagcttca aagccgcgag ctttttgttt
    10981 gccgaatagt tgatccgcac cgccggtaaa tgcactcgct gcactatttt gcttgcttgg
    11041 ctgcatcata atcgcaatga ccataacaat cgataaaacg atcacgattc ccaaaattaa
    11101 attatacata tcgcagcctc cttcccaaaa gatatcgtct ctttaacttt atcatagata
    11161 aaagtccgtg acaactctat ataaatggaa taacagctga aagttcaaaa ctttcagctg
    11221 ttattcttat gactgacgta agacgtcttg atattctggt ttcttttcaa actctttttt
    11281 tgcataaggg cataaaggaa tgatcttttt cccgtctcgt cttgctaatt ctactccagt
    11341 atacactagc ttttcagcta acttttgacc acgatatgct ggatctacaa atgtatggtc
    11401 aatgatcatg atgtctgggc ccgcatctga ccatgtcatt tctccgatct cttgttgttc
    11461 atcattgaat aaagcaagac gatttttttc ttctttgatt tccatatgcc gcactcctca
    11521 aataactgat atttttttat tcctacctta ttttagcagt ctcctgatct ataaacaatt
    11581 atttatcctc gaattactta caaaaaagaa gagaaaatta attcatcgtt attctttgtt
    11641 caataaatga aaaaatcact gtccaatagg ttttcggatc tttcatcgat gcttccaagt
    11701 gttctgcttc tggaacaagc agcagtgctt tatcccctgc cgtagcattt tgaatcgtgt
    11761 acgctgcttc tgtagggaca aaatgatctc tttcaccatg aatgaccaat aaaggcagat
    11821 ggttgctgcc aagctgtctc gtagctgatg cttgctttaa ggagtaaccg gcgtatttct
    11881 gggcataatg attcgcatat ctcatgacca tttttttagg aaagacagtg attttactta
    11941 acatatatcg gaattctgca taaacagaaa catatccgct gtctaaaata aatcctttga
    12001 ccgctgatgg aagtttttca ccacttgcca tcatgatcgt agaagctccc attgatccgc
    12061 cgtgtaaaat aatggacgca ttcggctgtt catctaagat gagttggatc caggcaatta
    12121 aatctagccg atctagccat cccattccga tgatttctcc ttcactttct ccgtgcgcac
    12181 gtaaatcagg tacaagaacg ttgaatcctt tctctgcata tcttttacct atatgagaca
    12241 tgtctctttt ccctgtgctt cggtaatcat gtacgcaaat gacccattta ttctgcttat
    12301 ttggatggat gaacatctga ccagctagtt tcaacccgtc ataacttttg attgttttgg
    12361 gtgttccctg ctcccaaaag ctttcacact cttcttctaa tttttgtcgt tttttttcta
    12421 ctgacgtgac tgcagcaggg tccatcaaac gatgaccagt gtcttcatac cagtcatatc
    12481 ctctctgtac tcccgctctt actaatttct ttgcataaaa ataagaaaga aaatggtaac
    12541 tgatccctat aattatcaga ataagaatga aataaaaaat aaccattgta cacccgccgc
    12601 tttctgccaa actataatct aactattatc caatgtaccg atccatagga ttctttattt
    12661 tcggcacacg taaattccat ttctttttca ttgtatcatt tacttaaagt tctcctcaat
    12721 agatttactt gtaagagagc atttacattc gcaataaaag ccagtatact aatttcgtgc
    12781 attataatca agacgctcgt cttggactgg ttcggaaact tcccagaata aaaaactaag
    12841 tatctctaaa gaaaggagat tggaaataat gcgaaaagta attattgatt gcgatccagg
    12901 gatcgacgac actttagctt tatcattagc tgtcaaatcc cctgatatcg aagtagttgc
    12961 tattaccgta gtttgtggga atgtccctgc agatataggt acgcagaatg tgctgaaatg
    13021 cctggaacgc tgtgatcgtt tagacattcc tgtttatcaa ggaatggaaa aacctttgaa
    13081 gcaacctttc atcagtgcac aagacactca tggtatggat ggtttagggg aaacaaattt
    13141 cccgatgatc ttgagaaaac aggcagaacc tctccatgcc gttgattttt tggctgatta
    13201 tttcaaagaa aagacaaata catcagtcat tgctttgggt cctttgacca atatcgcttt
    13261 tgctttgaaa gtcaacccaa atattgggaa acatatggat cgttttgtct cgatgggtgg
    13321 aacttacaaa agccatggta actgttcgcc agttgctgaa tacaattact ggtgtgatcc
    13381 tgaagcagct tcttatgtat tcgagaatct caaacaaacg atagaaatgg ttgggttaga
    13441 tgtgacaaga gaaatcgtct tgacaccgac tatcctcgaa tactgctgtc aaatgaatcc
    13501 tgaagaggga gagtatctaa aagcgatcac tcgtttttat tttgacttcc actggaaaca
    13561 agaaagaatt ttaggatgtg tcatcaatga tccgttagct gttgcttatt ttatcgaggg
    13621 ggccatctgt gaaggtttca aaagcttcac tgctgtggaa actcaaggaa taagtcgtgg
    13681 acaaacattg gttgatcgtt atgaattttg gcagaaacct gctaacagta aaattatgac
    13741 aaaagtagat actcgtcttt ttttccgaaa gtttttaacc gttcttttaa atgcacaaga
    13801 agaaacgatt atgaaagatt tggaaaggtt aaagatgggc taacaaaatg aataaaaaca
    13861 aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat tgggatcaat tatattggag
    13921 gaacgattgc tttatggctt cgtttgccaa tctatctcga ttctatcgga acgatttttg
    13981 ctggtgcatt gcttgggcct gtacctggaa tgctgacagg attgtccagc ggtattttaa
    14041 gtggagtgac tacagatatt ttttctctgt attattcgcc aatccaaatt ctcacaggct
    14101 tgcttgctgg attgattttg ccaagatatc tcggcaaaga taaaaacagc ttgaaactgt
    14161 tattactcgc tttgattttg tcggtacctg gaacgatcgt ttcttcgatc attaccgttc
    14221 agttatttgg cgggatcacc tcctcaggat ccagtatgat cgttcagcta ttacatgggt
    14281 taggtttaag tcagacaaca agtgtgatct tggtacaggc aggtacagac taccttgatc
    14341 gtttgttgtc tatcgtcgtt gtttcattag tcatcactat gttgccacaa cgcttgatat
    14401 attcaaaaag aaaaataggc tgaataaaca gagctggatt agcaaaataa tgctaatctg
    14461 gctctgtttt ttcatttatt aaaaaaatct tacgctaagt aggataaaat aattccttga
    14521 tatttttcaa tcatatccaa ataattatcg atttcaacat actcatctac ttgatgaggc
    14581 aaagtaacga caccaggccc aaagacgaca aagtcgaagg aatggttagc tttcgtaaat
    14641 tcggctgcat ccgttgttgc tgccgctcca actaaaggta atggctggct gaattgttgc
    14701 tgaatgcaat gaatcaaagg agaatctgga tcagctttga caggtatttt gttgtaatcg
    14761 atcatcaatt ccaaatgata gtctgtctct tgattcaatt cattgacgat tgattgcagc
    14821 aatgcaatga ttttatcatt tgggtattct ggaatcgatc gaatgttccc ttggagctgc
    14881 gcatggcttg ggatgctgtt gacttgattc ccgccactga tcaatgtaac attatggatt
    14941 gtttttccta ggacaggatt ctcgattgtt tcagcgaggt gattcatttc agcatttgct
    15001 ttcgtaatga attcatttag gtgattgatt gcattataac cttggtctgg catagagcta
    15061 tgcgcttctt ttccgtgcga ggtgactgta tagttgattg atcccatgtg cgtatacatc
    15121 aaactgtaat tagttggctc tccgatgatc aaggcatcta agtcatctac atagcctgct
    15181 ttcgtgagct gttccccacc tagttcaccc acttcttctc cgacagtggc taataatttg
    15241 actgttccgt taaatggttt tcctgactct ttcagttcga tcatggcaat caccattgct
    15301 gccaagccac tcttcatatc tgtagcacca cgtccgtaca aacggttccc atggatttct
    15361 gctgcaaagg gtgcataggt ccaagacgat tcatcaccag ccgccacgac atccatatgc
    15421 ccagataggc ctaacacttt tcctgattgg cccttttggt aacgagcaat caaattatct
    15481 ctaccatctc tgtaagaaac aatctctcct gttatgtcat gctttgccaa aagcttattc
    15541 aaataggctg ctacttcacc ttcgtttcca ttcactgatt taattcggat gatctcttgt
    15601 aaaatcgcga ttttttcttc ttttttcatc taatcctctc ccttgccatt tctttttatt
    15661 gtatttgagt gtgctcgaat tttcaaatag gagcaaccaa agtaaaaatg aagaaagaga
    15721 gccactttat gaaaaatatg ctgctacttc cctcctattt tatttatacg agaggacttg
    15781 ctcatagatt cctattctac tgttctatcc ttaaaagctc ttatatgatg ctagttattg
    15841 ttagctttcc tttttcaagg atgatctttt cttgtaccat tctttgtaaa catcatatac
    15901 agaagctagt tgcaaaaaaa actctctacc tgctgatatt
//
LOCUS       DAZBTH010000057        15917 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000057 DAZBTH010000000
VERSION     DAZBTH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.058"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..796
                     /locus_tag="ACHGNW_001765"
     CDS             <1..796
                     /locus_tag="ACHGNW_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926054.1"
                     /translation="FYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVR
                     KPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLN
                     KKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILL
                     GDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDS
                     GQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1370..1939
                     /locus_tag="ACHGNW_001766"
     CDS             1370..1939
                     /locus_tag="ACHGNW_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN6926055.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1926..2786
                     /locus_tag="ACHGNW_001767"
     CDS             1926..2786
                     /locus_tag="ACHGNW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6926056.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2976..3680
                     /gene="yycF"
                     /locus_tag="ACHGNW_001768"
     CDS             2976..3680
                     /gene="yycF"
                     /locus_tag="ACHGNW_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN6926057.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3685..5520
                     /gene="walK"
                     /locus_tag="ACHGNW_001769"
     CDS             3685..5520
                     /gene="walK"
                     /locus_tag="ACHGNW_001769"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN6926058.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5517..6833
                     /gene="yycH"
                     /locus_tag="ACHGNW_001770"
     CDS             5517..6833
                     /gene="yycH"
                     /locus_tag="ACHGNW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN6926059.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6834..7703
                     /locus_tag="ACHGNW_001771"
     CDS             6834..7703
                     /locus_tag="ACHGNW_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN6926060.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7758..8567
                     /locus_tag="ACHGNW_001772"
     CDS             7758..8567
                     /locus_tag="ACHGNW_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6926061.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8670..9299)
                     /locus_tag="ACHGNW_001773"
     CDS             complement(8670..9299)
                     /locus_tag="ACHGNW_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6926062.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9469..10761
                     /locus_tag="ACHGNW_001774"
     CDS             9469..10761
                     /locus_tag="ACHGNW_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6926063.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10837..11499)
                     /locus_tag="ACHGNW_001775"
     CDS             complement(10837..11499)
                     /locus_tag="ACHGNW_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6926064.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11724..12008
                     /gene="groES"
                     /locus_tag="ACHGNW_001776"
     CDS             11724..12008
                     /gene="groES"
                     /locus_tag="ACHGNW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN6926065.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12059..13684
                     /gene="groL"
                     /locus_tag="ACHGNW_001777"
     CDS             12059..13684
                     /gene="groL"
                     /locus_tag="ACHGNW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN6926066.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13773..14912)
                     /locus_tag="ACHGNW_001778"
     CDS             complement(13773..14912)
                     /locus_tag="ACHGNW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926067.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15023..15607)
                     /locus_tag="ACHGNW_001779"
     CDS             complement(15023..15607)
                     /locus_tag="ACHGNW_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN6926068.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15734..>15917)
                     /locus_tag="ACHGNW_001780"
     CDS             complement(15734..>15917)
                     /locus_tag="ACHGNW_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFN6926069.1"
                     /translation="KIAILILLNIYIENELTDESQFNLEKFMEYYSIQNKLRYTCYAV
                     CQCYFKKKYSYARQYV"
BASE COUNT         5213 a         2705 c         3280 g         4719 t
ORIGIN      
        1 gttttatcaa tatatgttaa atgataactg tacagcaaat acggttattc attatcacgc
       61 atttatacgc aaagctcttc aagaggctgt tattacagaa ttgataagta caaatgtagc
      121 agataaagta agaaagccaa aaaagaaaca atttgtttct caagtttata atcaacaaga
      181 gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc tatttggtag ttcttttaac
      241 agcattttat ggattgcgaa gaagtgaagt tttaggatta aaatggtctg ctattgattt
      301 tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga atatgtcaca
      361 gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac ctttggttga
      421 aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc gtattctttt
      481 aggagatgat tatattttag aagataatga gtacgtattt actatggaag atggacggtt
      541 gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa atggattgaa
      601 aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag atagtggcca
      661 gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa ctgctaattt
      721 atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag aaaacgcatt
      781 tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt cttgttttta
      841 gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt cacaaactaa
      901 aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg gttaagagtc
      961 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
     1021 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
     1081 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
     1141 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
     1201 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
     1261 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
     1321 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
     1381 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
     1441 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
     1501 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
     1561 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
     1621 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
     1681 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
     1741 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
     1801 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1861 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1921 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gttccagaag
     1981 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     2041 tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg ccagaggaaa
     2101 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     2161 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     2221 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     2281 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     2341 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     2401 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     2461 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     2521 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     2581 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     2641 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     2701 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     2761 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     2821 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2881 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2941 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     3001 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgacgttt
     3061 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     3121 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     3181 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     3241 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     3301 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     3361 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     3421 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     3481 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     3541 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     3601 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     3661 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     3721 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     3781 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3841 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3901 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3961 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     4021 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     4081 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     4141 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     4201 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     4261 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     4321 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     4381 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     4441 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     4501 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     4561 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     4621 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     4681 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     4741 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     4801 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4861 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4921 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4981 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     5041 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     5101 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     5161 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     5221 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt gattttaagt
     5281 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     5341 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     5401 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     5461 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     5521 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     5581 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     5641 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     5701 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     5761 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     5821 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5881 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5941 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     6001 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     6061 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     6121 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aagaagtata
     6181 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     6241 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     6301 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     6361 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     6421 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     6481 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     6541 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     6601 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     6661 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     6721 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     6781 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6841 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6901 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6961 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     7021 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     7081 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     7141 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     7201 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     7261 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     7321 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     7381 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     7441 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     7501 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     7561 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     7621 aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     7681 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     7741 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     7801 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7861 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7921 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7981 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     8041 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     8101 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     8161 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     8221 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     8281 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     8341 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     8401 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     8461 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     8521 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     8581 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     8641 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     8701 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     8761 gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     8821 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8881 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8941 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     9001 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     9061 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     9121 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     9181 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     9241 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     9301 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     9361 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     9421 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     9481 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     9541 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     9601 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     9661 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     9721 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     9781 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9841 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9901 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9961 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
    10021 gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
    10081 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
    10141 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
    10201 gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca agagttgacg
    10261 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
    10321 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
    10381 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
    10441 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
    10501 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
    10561 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
    10621 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
    10681 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
    10741 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
    10801 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10861 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10921 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10981 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    11041 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    11101 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    11161 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    11221 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    11281 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    11341 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    11401 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    11461 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    11521 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    11581 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    11641 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    11701 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    11761 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    11821 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11881 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11941 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    12001 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    12061 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    12121 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    12181 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    12241 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    12301 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    12361 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    12421 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    12481 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    12541 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    12601 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    12661 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    12721 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    12781 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12841 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12901 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12961 cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt
    13021 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    13081 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    13141 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    13201 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    13261 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    13321 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    13381 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    13441 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    13501 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    13561 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    13621 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    13681 gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt tgttttattt
    13741 taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt atcaattact
    13801 tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt tgcatcagaa
    13861 tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa tagtgaaacg
    13921 tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc ttcctctact
    13981 ttatggagta tagcgttaaa tgagtgcagc gcaagcggag tgccacttgt agatataaat
    14041 atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat cacactttca
    14101 atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt attttttgga
    14161 gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc attaatggaa
    14221 attttcccat cctcatagtc cttcatttgc aaagctagca attccccata tctcaaacca
    14281 gttaaatata gaaactcagc tattatgccg tgtagttttc tgcgaggatt ggagtatagc
    14341 tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttattcat agaaagcctt
    14401 cttttttctt cttccacttt tttaggatgg attttaactg ctagcgcagg gtttctttga
    14461 atgtattttc tatcgattgc atagtttagc ataacagata gagttgtttt tgtttgtttt
    14521 gtgtagttca gtgagaggtc accaaacgta tacatatctt caattatctt attaatgagt
    14581 gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt gcttagaaac atgtttcatc
    14641 atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata ttcataccat
    14701 tctttataca gctcaccaaa agtgatatct gatttattgt aatcttcgag tgcttcttta
    14761 atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt acttgtaagt
    14821 gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc aataaacttg
    14881 aattggccat ctttggtctg ttcaacccac acttttaaca tctcctatca tttgctataa
    14941 taggcataac aaatagacct atataggttt gttttctaaa agcacgctct tactctggac
    15001 ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg
    15061 atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag
    15121 ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct
    15181 ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat
    15241 cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa
    15301 attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt
    15361 ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag
    15421 aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc
    15481 caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata
    15541 cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt
    15601 ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt
    15661 atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    15721 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    15781 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt
    15841 tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
    15901 ttagaatagc tatttta
//
LOCUS       DAZBTH010000058        15803 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000058 DAZBTH010000000
VERSION     DAZBTH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.059"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..783
                     /locus_tag="ACHGNW_001781"
     CDS             31..783
                     /locus_tag="ACHGNW_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6926070.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            780..1694
                     /gene="pfkB"
                     /locus_tag="ACHGNW_001782"
     CDS             780..1694
                     /gene="pfkB"
                     /locus_tag="ACHGNW_001782"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN6926071.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1712..3643
                     /locus_tag="ACHGNW_001783"
     CDS             1712..3643
                     /locus_tag="ACHGNW_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN6926072.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3754..5313)
                     /locus_tag="ACHGNW_001784"
     CDS             complement(3754..5313)
                     /locus_tag="ACHGNW_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN6926073.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5468..7702
                     /gene="pcrA"
                     /locus_tag="ACHGNW_001785"
     CDS             5468..7702
                     /gene="pcrA"
                     /locus_tag="ACHGNW_001785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN6926074.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7719..9755
                     /gene="ligA"
                     /locus_tag="ACHGNW_001786"
     CDS             7719..9755
                     /gene="ligA"
                     /locus_tag="ACHGNW_001786"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN6926075.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9861..10166
                     /gene="gatC"
                     /locus_tag="ACHGNW_001787"
     CDS             9861..10166
                     /gene="gatC"
                     /locus_tag="ACHGNW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN6926076.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10166..11638
                     /gene="gatA"
                     /locus_tag="ACHGNW_001788"
     CDS             10166..11638
                     /gene="gatA"
                     /locus_tag="ACHGNW_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN6926077.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11635..13065
                     /gene="gatB"
                     /locus_tag="ACHGNW_001789"
     CDS             11635..13065
                     /gene="gatB"
                     /locus_tag="ACHGNW_001789"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN6926078.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13075..14130
                     /locus_tag="ACHGNW_001790"
     CDS             13075..14130
                     /locus_tag="ACHGNW_001790"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6926079.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14241..15614
                     /gene="rlmD"
                     /locus_tag="ACHGNW_001791"
     CDS             14241..15614
                     /gene="rlmD"
                     /locus_tag="ACHGNW_001791"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6926080.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15617..15745
                     /locus_tag="ACHGNW_001792"
     CDS             15617..15745
                     /locus_tag="ACHGNW_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6926081.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5372 a         2900 c         3474 g         4057 t
ORIGIN      
        1 gctattattt gtattgaggt gatcgtgaaa atgcttacag aggaacgcag acaaaaaatc
       61 ctagacttgc ttgaacagca gaaaatcgtg aaatcacaag atcttgtaaa ccttttgaat
      121 gcttctgaat caacgataag acgtgattta caagagctgg agaatagtgg gtttctagaa
      181 agagttcatg gcggagctaa gaaagagcaa ttattgaatt ttgagcagaa tatgagtgaa
      241 aaatcactca aaaacgttca cgaaaaacag caaatcgcca aatcggcagc tgaaaccatt
      301 aatgacgaag atgtcattta tttagacgct ggctctacta cgttagaaat gattcctttt
      361 ctgcaaggaa aacagatcac agtagtgaca aattccgttc aacatgcggc aaatttagtt
      421 gatttggagc ttgctaccat tattttaggt ggaacgataa agctggcaac taatgctgtt
      481 ttaggatcta atgcgataga acaactaaaa aattttcatt ttaataaagc atttatggga
      541 atgaacggtg cccatcttga acaaggattc acgactccgg atccagaaga ggcagctgtt
      601 aaacgtttag ccatgaaaaa ttccgaagag acatttgtat tgattgatca taccaaattc
      661 aaccagttgt ccttcacctc tgttgcacct ttggaagcag ccacgatcat aaccgagcgt
      721 tgtccattag aattcgttaa tgaattccgt gaaaagacaa caattaagga ggcaatacaa
      781 tgatttacac agtaacactt aatccatcaa ttgattatat cgtacgcgta gatgggttaa
      841 aactaggcga tttgaaccga atgaaggaag acttcaagtt acctggagga aaagggatca
      901 atgtttcccg tattttgaaa cggatcgatt ctcaatctac agcactaggg tttttaggag
      961 ggttcactgg cgaatttatt tcgaagtggc ttgaaaaaga acaaatccag tctttgttca
     1021 caaccgtaaa agaagataca agaatcaata tcaaactaaa atcagacact gaaactgaga
     1081 tcaatggctt aggcccatct atttcagatg aagaaatcga tgagttgaag caagcattag
     1141 gaacagtagg acaaggagat atcgtcgtat tgtcaggaag cgcgccagct agcctgcgaa
     1201 aaggatttta tcaagaactg attgatatca ttcgagaaaa aggtgcagat ttcgtgattg
     1261 atacaacagg tgatgaccta aaaaatgcac ttgttaagaa accgttgttg atcaaaccaa
     1321 acaatcacga attagctgag ctttacaaca caaccttcca atctgtagaa gaaatcattc
     1381 cgtatgggaa aaaattacta gctgaagggg cacagaatgt gctgatctca atggctggag
     1441 acggtgcttt gctcttcacg gaacaaggaa cttataaaac gaatgtatta gttcgcccat
     1501 tgaaaaattc agtaggtgca ggggattcaa tgattgctgg ctttattggg gcttacagcc
     1561 gtacgaaaga tccaatcgaa gcctttaaat ggggagttgc ttgcggaagc gcaacagctt
     1621 tttctgatga cttggcagct cgcgaattga ttgatgagtt gctgccagaa gttgaaatca
     1681 caaaaattga ttagtttcaa ggaggataat catgaatatc aaggatttaa tggtcaaaga
     1741 agcgatgatc atggatttgc aagcgactga taaaaaaggc gcaatcgatg aaatggtcca
     1801 aaaaatgttt gatgccggcc ggatttccga tatcgaaaca tataaagaag gaattctagc
     1861 ccgcgaagca caaacttcta caggtcttgg tgatggaatc gccatgcctc acgcgaaaaa
     1921 cagtgcagtt aaagaagcaa cagttttatt cgcaaaaagc caaaaaggag tagattatga
     1981 agccttagac ggacagccaa cttatctgtt tttcatgatc gcagctccag aaggcgcaaa
     2041 cgacacacat ttgcaagcat tagcagcact atctcgattg ctgattgatg cagatttcgt
     2101 aggaaaatta aaagaagcaa atacaccaga agaagtccaa gacttgttcc aaacagctga
     2161 agagcaaaaa gaagcagaag aaaaagcaga acaagaaaca caagctgcag aagcttctgc
     2221 tcatccagaa agaaaattcg ttgttgccgt tacagcttgt ccaacaggga tcgctcacac
     2281 ttacatggca gaagatgctt tgaagaaaaa agcgaaagaa atgggtgtgg agatcaaagt
     2341 cgaaacgaac ggttcagaag gaatcaaaaa ccgattgact gctgaagaca tcgcccgtgc
     2401 agaaggtgta atcgttgcag cagacaaaaa agtagagatg aatcgctttg acggaaaaga
     2461 actgatcaat cgtcctgtca gtgatggtat ccgtaaacca gaagagttga tcaatctagc
     2521 ccttagtggt actgcaccag tattccatgg tgatggaaaa gaaagcagtt cagaagaagg
     2581 aaatgcggat ggtactatcg gacaaagaat ttacaaagat ttgatgaacg gcgtatcaca
     2641 catgttacct ttcgttattg gtggaggaat cgctattgcg ctttctttca tgatcgacca
     2701 atttattggc gtgccacatg atcaattatc aaatctaggg aattacaatc aagctgctag
     2761 ctggttcaac caaatcggac aagctgcatt tggtttcatg ctgccggtat tagcaggatt
     2821 tattgcttca agtatcggtg accgccctgg tttgatcgtt ggatttgctg ctggtgcatt
     2881 agctaataca ggcggtgcag gattcttagg tgcactgatt ggtggattct tagctggtta
     2941 tgtcatcgta tttttacgta aattattcaa gaacttacca aaatcattgg atggaattaa
     3001 aacgatcctc ttttatccag tgtttggctt actgattact ggtttcttga tgttgctggt
     3061 caatgtccct atgaaagcaa tcaatgatgc attgaacggt ttcttgacaa gtatgagtgg
     3121 ttcgaatgct gctttacttg gtgcattatt agctggaatg atggcggcag atttaggcgg
     3181 tccaatcaac aaagccgctt atgtgtttgg tacagcaact ttagctacga ctgttgcaac
     3241 tggcggaagt gtcgtgatgg cttctgttat ggctggagga atggtacctc cattggcaat
     3301 tttcgttgct acacgtttgt ttaagaataa attcagcaaa acagatcaag atgctggttt
     3361 gacgaacatc gtgatgggac tatcgtttgt cacggaagga tcgattccat ttgcagcagc
     3421 tgacccgatt cgtgccattc caagttttat tattggttct gctttaactg gcggattagt
     3481 cggagctttc ggaatcaaat tactggctcc tcacggcggg attttcgttg tattcttact
     3541 gagtcacccg gtcatgtatt tggtgtttat tgctatcgga gcagtcgtat ctggtgtgat
     3601 ttatggttta ttgagaaaag ctcctgcagt agcagaagca tagaaataaa aaagaagagg
     3661 ctgggttttc ccagcctctt ctttttttat agaatcatat agaatcaagt agtgtagatg
     3721 aatagttgtt gtaaaatagt ttttccatgt tcgttaaaat gattgcttca aatttgtgat
     3781 agaagattta gcctcgattt cctgcttgat tcttggcaag tttgcttcat cagtcaagat
     3841 aaccaaaata tctcctacat tcatgatcgt atcaccgtga gtcaagattt cctgatttcc
     3901 tctgcgtata gagattagca gcatattttt aggccaattg aagtctcgta ccattaatcc
     3961 gtctaaagaa ctctctgccg ttactggaaa atcaaaagtt gtccttgcac cttttagtga
     4021 ggcggtctga gtatttccaa gtagacgttc caataatgat tcgtaaattg gattaccgcc
     4081 gagaaggtca ttgacgatat aagcaacaag agcagccaat cctaaaggca tcagatgatt
     4141 aagagagcca accatttctg tcactaaaat aattgctgtc agcggtgctt ttccaatcgc
     4201 tgtgaaataa ccagccatgg caataacgac aaaatccttg atgtagattg gatccatccc
     4261 gaaatcaacc ataatgtttc cataaaagct accgattaaa gcccccaatg tcaatatcgg
     4321 aaggaaaatc ccccctggaa gacccccacc gtaagaaatc atggaaaaaa cgaatcgaag
     4381 aacaaacaaa ccgataatca taatgattgg caaggaagca tgtccaaaag ctaaaacgat
     4441 ttgattgcct ccgcctagag tactaggcca taaataacca attggcaaaa tcagcaagaa
     4501 aggaacgaac ccgtgaaaat aagctggtaa aggcaatcgg ttgtaaaatt ttggcaaagc
     4561 cagcaagaca ttctgataaa taaaacctaa gatgcctaga agtactccta agatcactaa
     4621 ggaaccataa tatttcaacg gcaaagaagg aaggtcacct agatataaaa caggtctcaa
     4681 gccgaaaaag taaagagaga cgaagttagc agtgattgct gaagcaaatg atgtcagcca
     4741 aacaagtgga gaaaaggagt gatggatttc ttctaaaaca aagagtaatc ctgcgatagg
     4801 agcattgaat gctgctgcaa gtccgctact tgcaccgcta gaaattaaaa tcttttcttc
     4861 tgaagatgga ctccgaaaca attgtccagc tccttgtcca atggatgcgc caagctgtat
     4921 agaaggtcct tctctaccta gaaaaaggcc cgcaccaact gaaagtacac cgccaatgaa
     4981 ctttttccat aaaacagaga accagttcat tgataattcc ccgcgaagct gtccctctac
     5041 ttgtggaatc ccgcttccct tgatatccgg ttcatttttg accagataac ccacgaaaaa
     5101 tgctgcaagg atgcagcaaa tagctaggcc aattaagtaa atcggctgtt cccgactcat
     5161 ttcatagata cttcgaacta aatcgcttag cttttcgata gataatcgaa acaggctaac
     5221 gacgattcct gtcagcagtc caacaaatat tccttttaga ataaaatata tctgagtctt
     5281 atctaatttc ttgatttttc cttttttttc cattcaaacg cctcgctttc ttttaattta
     5341 gcgcgaaatc aacgaattga caaggaaaaa tccataaaaa gaaacaaatg attttgaact
     5401 agctagttta atgaaagcca agaaaatgct ataattaaca agattgataa aagaaaggat
     5461 gttgttaatg tcatccaaag ctgaattatt aaacgggatg aacccccgtc agaaagaagc
     5521 tgttcttcac actgacggac cattactgct aatggcaggt gcaggaagtg gaaagacgag
     5581 agtgctgaca caccgcattg cctatttgat tgaggaaaaa gaagtcaatc cttggaacat
     5641 cctagcgatt acttttacca ataaggctgc aaaagaaatg aaagaacgtg tcaatgcgat
     5701 tctcgcatct ggaggcgaag atgtctgggt atcgactttt cactcgatgt gtgtgcgtat
     5761 tttaagaaga gatgttgatt ttatcgggta taaccggaac tttacaatca tcgattcttc
     5821 tgaacagctg accttgatga aacggatact aaaagaactg aacattgatc cgaaaaaata
     5881 tgatcctcga agtatattag gaacaatctc tcaagcaaaa aacagcctgc agacccctca
     5941 ggattttgca aaaatgcaag gcagttatta cgaagagatt gcggcaaaat gttatgctgc
     6001 ttaccaaaaa gagcttcaat ataatcaatg tatggacttt gacgacctga tcatgaatac
     6061 gattcgatta tttgaagaac atcctgattc actcacgtat tatcaaaaca aattccatta
     6121 tattcatgta gatgagtacc aagatacaaa tcacgcacag tatacgctag tcaatttatt
     6181 ggcaggtaga tttaggaacc tgtgcgtcgt gggagatgca gatcaaagta tttacggctg
     6241 gcgcggtgca gatatgcaga acatcttgga ttttgaaaaa gattatccag atgctgcagt
     6301 aattttgcta gaacaaaact atcgctccac gaaaaacatc cttagcgcag caaaccaagt
     6361 cattgaaaac aatagcaatc gaaaaccaaa aaacttgtgg acagaaaaca aagaaggaaa
     6421 caagatcact tactaccgtg cagataatga acgagacgaa acccgtttta tcgtagaccg
     6481 gatgcaagaa gagattcgaa gcaatcatcg gaattacggc gactttgcca tcttgtaccg
     6541 gacgaacgca caatctcgtg taatggaaga aactctgttg aaagcaaata ttccttataa
     6601 aatggttgga gggcacaagt tctacgatcg taaagaaatc aaagatattt tagcgtattt
     6661 gaatgtgctg gtaaatcctc aagattcaat cagttttgag cgtatcgtta attcgcccaa
     6721 acgaggcatc ggaccaggat ctatcgaaaa actacgcagt tttgcttctc ttcatgaatg
     6781 gccactgctt gaagcagcgc aaaatgtgga tctggcaaat atcggtggaa aagcaggaca
     6841 gcaattaggt gcatttggag agatgatcca agaagtcact cagatgatcc catacttgac
     6901 agtgacagaa ttgacgaaag aggttttgga tcgtagcgga tatctagaag acttaaaaat
     6961 ccaaaataca ttagaagcac aagcacgcat cgaaaacttg gaggaatttt taacggtcac
     7021 acaagaattc gacaaacaat tcgaacagca aaatgaagaa gacgctgatg caccagaaga
     7081 aaaactaacg gtcttcttaa atgatctggc acttgtttcc gacattgaca atttagaaga
     7141 agatgcttcc caagtgacat tgatgacttt gcatgcagcc aaaggattag aatttccagt
     7201 tgtattcctg attggtctag aagagggggt ttttccttta tctcgagcat tgatggaaga
     7261 aagcgaactg gaggaagaac gccgtctcgc atatgtgggg atcacgcgag cagaagaagc
     7321 gttgtattta acaaatgctt tctccagaac attatatgga cggacgcaat ataaccgacc
     7381 ttctcgtttt gtagaagaaa tcgatcagga gttactggaa atcgaaggca tgcgtcctac
     7441 gcctaaaaaa acgcctgtat tcgctaaaaa aacagcatat agctataaac agccagagac
     7501 agcggttgtt ccctctaaaa gtgctactgg aggggaaaat aacagctgga aacctggaga
     7561 taaagtcaaa cacaaaaaat ggggtctagg aacagtcgtc cgagtaagcg ggacgtcgaa
     7621 ggatctagaa ctagatgtgg ctttcccaag tcaaggagta aaacgtcttt tggctgcctt
     7681 tgcgccaatt gaaaaagctt agtagtggag ggtaaatgat ggaacatgaa atgacgattg
     7741 acgaagctgc taaacgagca gaagaacttc gtacaagact gaaccaatgg tctcgcgaat
     7801 actacgtaga agataaacca acagtggaag actatgttta tgataaagaa tacgcagaat
     7861 tagtagcaat cgaggaacaa tatcctgatt tgatcacgtc tgattctccc actcaacgag
     7921 taggcggaaa agtattagaa ggttttgaaa aagtcactca tgatattccg ctttacagtt
     7981 tgaacgacgt tttcagcaaa gaagagctga tagcttttga tcaacgtgta caaaaagcag
     8041 tgggacgtgt agttgactat tgctgtgaac taaaaataga tggactttcc gtttctttgc
     8101 gttatgaaga tggaaatttc gtgagaggtg cgactcgcgg ggatggaact gtaggagaaa
     8161 acatcactga aaacctaaaa actgtccggt cggtcccaat caaattaaaa gaaccaatga
     8221 acatcgaagt aagaggcgaa tgtttcatgc caaaacggtc atttgtccaa ttgaaccaag
     8281 acagagaagc agagggaaaa gatatctttg cgaatcctcg gaacgccgca gctggttctt
     8341 tgcgtcagtt agattctaaa atcacagcga aaagaaatct ggacactttt ttatacacag
     8401 tagctgattt cggtcctatg caagcaaaaa cccagtatga tgcgctagaa gagctagaaa
     8461 aaattggatt ccacacgaat cgggaaaaac gtctatgtca ttcgattgat gaagtgtggt
     8521 catacattga agaatatcat gacaaacgtg tggatttgcc ctatgaaatc gatggaatcg
     8581 tcatcaaagt gaatgaattc tcgctacaag atcaacttgg ttttactatc aaagcaccga
     8641 gatgggcagc agcttacaag tttccgccag aagaagttga aacgctaatc gaaaacatcg
     8701 aatggacagt cggtcgaaca ggggtagtaa cgccaacagc tatcatgaca ccagtccgag
     8761 tagcaggaac gacagtaagc cgtgccagcc tgcataacgg tgattatatt aagttgaagg
     8821 atatccgctt gaaagataca gttctgattt ataaagcggg agacatcatc ccagaagttt
     8881 cgcaagtcgt gttagataaa cgacctaaag acagcgaaga ataccaattg cctacacatt
     8941 gtccagtttg tggaagtgaa cttgtccatt tggatgaaga agtagcctta cggtgcatca
     9001 atcctaaatg ccctgctcaa atgaaagaag gtttgaacca ctttgtttca agaaatgcga
     9061 tgaatatcga tgggttgggg ccgcgcgtac tagaacagat gtatgataaa aaactagtag
     9121 cagatgttgc tgatctttac aaattgacag aagaagagtt attgacgttg gacaaaataa
     9181 aagaaaaatc agcaaataat attttgacag caatcgacaa cagcaaagat aattcggtcg
     9241 agcggttgat tttcgggtta ggaatccgac atgtcggagc gaaagcagca aaaattttag
     9301 cagaacattt cggtgatcta gaaacattga gtaaaagtga ctatgagtcg attattgcgc
     9361 ttgatacgat cggtgatatc attgccgaca gtgtagtcac ttatttcagt aacgaagaag
     9421 tccatgaatt gatgaatgaa ttaaaacaag caggcgtcaa tttcgaatac aaagggcttc
     9481 gaaatgccca attgcaagaa gtcgagtctc cttttaaaga aaaaacagta gtccttactg
     9541 gtaaactaac acgatttact cgtgaagaag caaaagagac gatcgaaaat cttggcggga
     9601 aagtgactgg tagcgtatcg aaaaaaacgg atatcgtggt agcaggagaa gatgctggca
     9661 gcaagctgac caaagcacaa gaattaggaa tcgaagtttg gacagaagat caaatggcag
     9721 atgcattggc taaaagtcgc tcagtagaag aatgaaaaat gagttttttc agacaaattt
     9781 caataaacaa tttcagacac ggcactcttg tgttaaaata gagagcaaag tttttaactc
     9841 gtttagaaag aaggggtagt atggcgatta gtgaagaaca agtgaaacac gttgccaaat
     9901 tagcaaaact tgcgttcgca gatgaagaac ttgcaagttt taccgaccaa ttaggtaaaa
     9961 tcatcgatat ggttgaaatg ctggaagaag tagatacgga aggtgttcca tttacgtcga
    10021 atgtggttga aacagtaaac gtgatgcgac aagatcgggc gcaagctgga tggaaccgcg
    10081 atgaattatt gaaaaatgtg ccggaaacag aagacggctt tatcaaagta cccgcaatca
    10141 tcgataatgg ggaggctggc gcataatgac agaattacac aatcgttctc tagaagaact
    10201 gcatcatatg cttgtatcga aagaaatcac tgtacaagat ttgacaaaaa caacatttga
    10261 acgaatcaaa gaaacagagc ctgaggtcga ggcattcatt acattgaacg aagaaaaagc
    10321 attggaacag gcaaaagctt tagatgaaaa agggattgat gaaagcaacg tccttgctgg
    10381 tattccaatt ggtatcaagg ataatatcgt aacaaaagat atcttgacaa ccgctgcatc
    10441 taagatcctt tacaattttg aaccaattta tgatgcaacg gtcatggata aaatctatca
    10501 agcagatatg atccctgttg gtaagctaaa catggatgaa tttgctatgg gtggaagtac
    10561 agaaacttct tatttcaaga aaacaaaaaa tgcttgggac ccaacaaaag tacctggtgg
    10621 ctcttctggt ggttctgcag cagcagttgc agctggacaa attccagttt ctttaggtag
    10681 tgatactggg ggaagtatcc gccaaccagc agcttttaac ggcatcgtag ggatgaaacc
    10741 tacttatggc cgtgtctcac gttttggttt gattgccttt gcttcatctc ttgaccaaat
    10801 cggaccaatg acccgtacgg tgaaagataa tgcattagca ttgacagcta tcagcggtta
    10861 tgacgaaaaa gacggtactt cttcaggcgt ttcagttcct gattttgcag agggactgac
    10921 aggcgacatc aaaggaatga agattgcttt acctaaagaa tacttaggcg aaggtgtggc
    10981 ccctggcgta aaagaagctg tattgaaagc agcagaaaca ttccgttcat taggtgctgt
    11041 tgtggaagaa gtcagcttgc ctcattcaaa atacggtgtg gctgtttatt atatcattgc
    11101 ctcatcagaa gcaagttcca atttgcaacg attcgatggt atccgctacg gttatcgctc
    11161 tgaaaatgtg aaatcattag atgatgtata tgtcaactca cgttcagaag gattcggtga
    11221 agaagtaaaa cgccgtatca tgttaggtac attctcctta agcgcgggtt attatgacgc
    11281 ttacttcaaa aaagcaggac aagtccgtac attgatcaaa cgagattttg aaaaagtctt
    11341 tgcagattat gacttgatca tcgggccatc ttcaccaaca gttgcattcg gaatcggtga
    11401 gaacattaac gatccaatca caatgtacat gagtgacatc ttaacgatcc ctgtcaactt
    11461 ggctggactt ccaggtatgt ctgtaccggc aggtttatca gaaggattac cagttggatt
    11521 gcagatcatc ggtaattatt ttgatgaaaa aacgatgtat caagcggcat atgcgtttga
    11581 acaagcaaca gaattccata aacaacaacc agctatttta ggagggaaag acgaatgaat
    11641 tttgaaacag tcattggact agaagtccac gtcgagttaa aaaccaactc aaaaattttc
    11701 tctccagcgc cagctcattt tggtgcagaa ccaaacagca atacaaacgt gatcgactgg
    11761 ggttatccag gcgtgctgcc agtaatgaat aaagctgcgc tggaattcgg gatgaaagca
    11821 gctttggcat taaactgtga aatctctaaa gatactcatt ttgaccggaa aaattacttt
    11881 tacccagata atccaaaagc atatcaaatt tcccaatttg atcaaccaat cggtcatgac
    11941 ggatggatcg aaatcgaagt agaaggtaaa aagaaaaaaa tccggatcga acgtgtccat
    12001 ttagaagaag atgctggaaa aaacatgcat ggtattggcg gttattctta cgttgatttg
    12061 aaccgacaag gcacgccttt gatcgaaatc gtttctgaag ctgatatgcg ttcaccagaa
    12121 gaagcatacg cttatctaga agcattgcga tcaattatcc aatttacaga agtcagcgat
    12181 gtgaaaatgg aagaaggctc tatgcgctgt gatgccaata tttctcttcg cccttatgga
    12241 caagaagagt tcggtacgaa agcagaattg aaaaacttga actctttatc tttcgtgaaa
    12301 aaaggtttag cttttgaaga aaaacgtcaa gcaaaagttc ttctttcagg aggagaaatc
    12361 caacaagaaa cacgccgttt tgacgaaaca acgaataaaa cagttttgat gcgtgtcaaa
    12421 gaagggtcta gtgattatcg ttacttccca gagcctgata tcccgaaatt cgtgatcgat
    12481 gacgaatgga ttcaaaaagt aaaagctagt ttgcctgaga tgccagcttc tcgtcgcgaa
    12541 cgctatatcc gtgagtttgg acttccagag tatgacgcaa tggtattgac acttacaaaa
    12601 gaaatgtctg atttttttga agctgttttg gaaaatggtg cagatgccaa gcaagcatct
    12661 aactggttga tgggtgaagt ttctgcttac ttgaacagtg agaaactgga attagctgag
    12721 acaaaattga cacctgagaa cttagcagga atgattcgtt tgatcgccga tggaacgatc
    12781 agctcaaaaa tcgccaaaaa agtcttccgt gaattgatca caaatggcgg agaagcccaa
    12841 gaagtagttg aaaaaaatgg ttgggtgcaa ttatctgatc cgaacaaact actgccaatc
    12901 attaatgaaa ttttggataa caaccaacaa tctgtcgatg atttcaaaaa tggaaaagat
    12961 cgtgcggttg gcttcctagt cggtcaaatc atgaaagcaa ctaaaggaca agcaaatcct
    13021 ggtgtcgtga ataagctact taaagatgaa ttagcgaaac gatagaggct gaaaatgaaa
    13081 aaagcacgcg taatttataa ccccacctcg ggaaaagaat tattgaaaaa aaatttggcg
    13141 gatatcctac aggcattaga agaagcagga tttgaagcca gcgcctatgc aactacacca
    13201 gaaccagatt cagccaagaa tgaagcatgt agagtcgcag aactaggttt tgatctagta
    13261 gtggcagctg gtggcgatgg cacaatcaac gaagtggtta atggaatcgc tccgcttgaa
    13321 caccgtccta aaatggccat catcccagct ggtacaacga acgactatgc tcgagcatta
    13381 aaaattccta gagataatat taaagcagca gcagaagtga taaaaaaaca ccaaaccgta
    13441 aaaatggaca tcggcctttc tgataacagt tacttcatca atatcgctgc aggtggctat
    13501 ttgacagaat tgacttatga agttccatca gaactaaaga gtatttttgg ttatcttgct
    13561 tatcttgcga aaggagcaga gatgctccct cgagtaaagc caatcaaaat gcgcttgaag
    13621 tatgatgaag gggaatatat cggaaatgct tcgatgtttt tcctagggct gacgaactct
    13681 gttggaggat tcgaacagat cgcgccagat gccaaattgg atgatgggaa gttttcttta
    13741 atcattgtga agacagcaaa tatctttgag atcttgcata ttgcagcact catgctgaat
    13801 ggcggtcgac atgtcgatga tccaagaata atctatacca aaacaagcag tttgttcgtt
    13861 gaaacagaag atgatatcaa tcgaccagta atgatcaact tagatggtga atacggcggg
    13921 gaagcaccga tgcatttcca aaatcttcat caacacattg agttctttgc caataccgat
    13981 cagattcctg atgaagcaat cacaggtaca gatgaagaag aattagaggt tgtcagcaaa
    14041 gaattcgtaa aagaagtcga acgtttaacg gatgaagata tcgacggtga cggaaaaata
    14101 gcagacgaaa cacctaatca aaagaattaa tcaaaagccg gtgctgaacg tagtaaggtc
    14161 agtgccggtt ttagttgtgt aattagctga tttatagtag agttcagtag gattgaaaaa
    14221 taggcttaaa ggagcaaaat atgacagaac agcaaatagt aaagaaaaac gaacgatata
    14281 cagtcgaaat cgtcgacttg agttatgaag gaatgggggt agcaaaaatc gatggttatc
    14341 cattatttat cgaaaatgca ctccctggag aagaagtaga aatactagta gtaaaagcag
    14401 gcaataagtt tggatatgga aaagtcgaaa catttattac acaatcacct gatcgtcagc
    14461 ctgtagaaaa caatgcatta cttcgcatag gtatcgcacc tttagcccat ttgaagtatg
    14521 aacaacagct tgtcttcaaa caaaaacaag tagagcatgt aatggcgaag attgctaaga
    14581 tgccagaagt tcctgtactt ccaacggtcg gaatggcaga accattcggc taccgtaata
    14641 aagcacagat ccctgttcgc cgtatcgatg gacagttagc caccggtttt tacaaaaaga
    14701 atagccatga tctagtccct atcgaagatt tttatatcca agatcctgtc atcgatcaag
    14761 caatcaaagt agtcagagat atcttgcaac ggttccaagt acgaggatat aatgaagaaa
    14821 aaaatgaagg acagcttcgt cacatcatta tccgtcgtgg tcattattcc catgaaatga
    14881 tggtcgtttt agttactcgt aaagaaaaat tctttaaatc caaggaaatt gctgaagcta
    14941 ttcatgaaga attgccagaa gtggtttcgg tcatccaaaa tatcaatgaa gaaaaaacaa
    15001 atgtgatttt aggagacaaa gaaaaagttt tatatggcag agactatatc gaagatcagc
    15061 tattaggaaa aacctatcga atctcagcaa agtcatttta tcaagtaaat actcaacaag
    15121 cagagtatct gtaccaaaca gccatcgagt ttgccgagtt agataaaaaa gacattgtga
    15181 tcgacgctta ttcaggaata ggtacgattg gcttgtcttt agcagacaga gtggataagg
    15241 tgtatgggat ggaagttatt ccagaagcaa ttgaagatgc acagttcaat gctttaaata
    15301 ataaaatcga aaatgttcat tatgaagtcg gaaaagcaga aaaagttatg aaaaaatggc
    15361 aagaaaaagg gatcaaacca tctgtgatcg tagttgaccc tccacgaaaa ggattagatc
    15421 atcattttat tgaatcagca atcgaaatgt caccagaacg aatcgtctat atctcatgta
    15481 atccggcaac ttttgcaaga gatgctaagt tatttgcaga agcaggttat gccgtgaaga
    15541 aagttcagcc tgtagatctt ttcccgcaaa cccatcatat cgaattagtc gcttcattcg
    15601 ttaaaggaga ataaaaatgg accaaaaaga aaaaatgaaa gaattgatca aaaagaaaca
    15661 aggtggaggc cgatccaaac aaatggaaac acctaagaat gatcgtaaaa acatgagaaa
    15721 aggccctaaa atctttaata aatagacgtg ctaaagaaag actaagaagt gttaactttt
    15781 gttattgttt cttagtcttt tta
//
LOCUS       DAZBTH010000059        15730 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000059 DAZBTH010000000
VERSION     DAZBTH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.060"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACHGNW_001793"
     CDS             <1..243
                     /locus_tag="ACHGNW_001793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926082.1"
                     /translation="LKNLFEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPE
                     YVLGISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
     gene            complement(289..655)
                     /gene="ssrA"
                     /locus_tag="ACHGNW_001794"
     tmRNA           complement(289..655)
                     /gene="ssrA"
                     /locus_tag="ACHGNW_001794"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(826..1599)
                     /locus_tag="ACHGNW_001795"
     CDS             complement(826..1599)
                     /locus_tag="ACHGNW_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6926083.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1743..2093
                     /locus_tag="ACHGNW_001796"
     CDS             1743..2093
                     /locus_tag="ACHGNW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6926084.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2074..2790
                     /locus_tag="ACHGNW_001797"
     CDS             2074..2790
                     /locus_tag="ACHGNW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN6926085.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2790..4238
                     /locus_tag="ACHGNW_001798"
     CDS             2790..4238
                     /locus_tag="ACHGNW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6926086.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4308..4817)
                     /locus_tag="ACHGNW_001799"
     CDS             complement(4308..4817)
                     /locus_tag="ACHGNW_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926087.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4807..5532)
                     /locus_tag="ACHGNW_001800"
     CDS             complement(4807..5532)
                     /locus_tag="ACHGNW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926088.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5755..7875)
                     /locus_tag="ACHGNW_001801"
     CDS             complement(5755..7875)
                     /locus_tag="ACHGNW_001801"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6926089.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8105..9283
                     /locus_tag="ACHGNW_001802"
     CDS             8105..9283
                     /locus_tag="ACHGNW_001802"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN6926090.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9299..10057
                     /gene="aroD"
                     /locus_tag="ACHGNW_001803"
     CDS             9299..10057
                     /gene="aroD"
                     /locus_tag="ACHGNW_001803"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN6926091.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10080..10565
                     /gene="ybaK"
                     /locus_tag="ACHGNW_001804"
     CDS             10080..10565
                     /gene="ybaK"
                     /locus_tag="ACHGNW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN6926092.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10636..11889)
                     /locus_tag="ACHGNW_001805"
     CDS             complement(10636..11889)
                     /locus_tag="ACHGNW_001805"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6926093.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12007..13356
                     /gene="gdhA"
                     /locus_tag="ACHGNW_001806"
     CDS             12007..13356
                     /gene="gdhA"
                     /locus_tag="ACHGNW_001806"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN6926094.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13468..14814
                     /locus_tag="ACHGNW_001807"
     CDS             13468..14814
                     /locus_tag="ACHGNW_001807"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN6926095.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15122..15508)
                     /locus_tag="ACHGNW_001808"
     CDS             complement(15122..15508)
                     /locus_tag="ACHGNW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6926096.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15557..>15730)
                     /locus_tag="ACHGNW_001809"
     CDS             complement(15557..>15730)
                     /locus_tag="ACHGNW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN6926097.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5139 a         2814 c         3163 g         4614 t
ORIGIN      
        1 ttaaaaaact tatttgaagg aacatgctta aaccataatt gtgtttttcc ttccatcagc
       61 ataaaagttc acctcaaaaa gagtgtacga acaaaagttc tttttgtaaa ccttaaaata
      121 gattttcctg aatacgtatt ggggatttct aaatgtttga atgttttttt gaatgcaaaa
      181 tcaatgcaaa aaattttgta tttctctact tttctctcct tgaccaaaat aaaaaaacgt
      241 tgatttaaca acgtttttca tacatgttta tctgtctcaa ccatacatat atggagacgg
      301 cgggagtcga acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact
      361 catttaagga ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat
      421 gttctctttt tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct
      481 tactcgagca catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag
      541 cgtaagagtt gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt
      601 aacctacgaa acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag
      661 ctaacaaggg ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt
      721 caataaaaaa gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa
      781 aataagcgat tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa
      841 atacttcaat gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag
      901 ttcttttaat ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa
      961 ggcaaaatca ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc
     1021 tttttgcaga cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga
     1081 gtaaggacca ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc
     1141 tgattgagat aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat
     1201 ttccggaaca tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt
     1261 acaccagatt ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc
     1321 atacattttt tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac
     1381 tcgaatgaaa gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga
     1441 gtggatttct gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa
     1501 tgcgttcata gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc
     1561 atcaagatca aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt
     1621 ggaataagtt tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg
     1681 aacatattgg ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa
     1741 gaatgatttt tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc
     1801 tgccctcaaa aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag
     1861 acgctcattg gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac
     1921 tgatggcatt gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg
     1981 aaatgcttct gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac
     2041 agaaatatga cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat
     2101 caatctgttt ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc
     2161 aagtatgatg gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg
     2221 attacactag acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc
     2281 aaagtttcaa gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag
     2341 gtgatcggct tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg
     2401 cttattgctc gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa
     2461 gcagcagttt cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa
     2521 acacgagaag cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat
     2581 ctattgttta ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa
     2641 ttagtctggg attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa
     2701 ttacgacaaa aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc
     2761 tacaaattcg atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt
     2821 tttgcggaat ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa
     2881 aacaaacgat tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa
     2941 agacaacgca aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa
     3001 atgcattgct gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg
     3061 ataaaaacga acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag
     3121 ctgatcaatg gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata
     3181 ttttaggaca agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat
     3241 tctatggagc acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa
     3301 ttacactgaa cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct
     3361 atgcttttgc agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag
     3421 tgacgaatgg taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt
     3481 tagcagatga tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag
     3541 aacaagaaaa tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc
     3601 ttacgacaat caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc
     3661 gagaaaacgc gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg
     3721 aaaacttgga ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca
     3781 atggaacaga aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag
     3841 gagatacatt gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat
     3901 ttgtccaaat catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa
     3961 tcacgattac tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa
     4021 ttggaatgtc tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt
     4081 caagaaaaaa tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg
     4141 tacgcttgca taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg
     4201 ctgttcgttt gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc
     4261 atgaatcgat cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc
     4321 tctaaacgat caatataagg actttgagca atagtagcat catgagtaaa gagtaagata
     4381 ccttgatttt cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat
     4441 acactaagtt catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca
     4501 tgaagtaatt gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc
     4561 aaggaaggag agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt
     4621 tccggtacat ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt
     4681 ttttggatga tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc
     4741 aatattttat gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc
     4801 aaatattcag gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt
     4861 gaaagggaat agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa
     4921 tcccttaagt atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg
     4981 ttgatccatc ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag
     5041 ttcagtagtc tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg
     5101 gaagcttacg atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg
     5161 ccagaaaata aaaaggaata atttggtttt agaaagacca gtcaggcgcc aattgatcaa
     5221 ttcctgcttg ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag
     5281 aaaaaataaa ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag
     5341 aagctctgta gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa
     5401 agccgattgc aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga
     5461 aaataaacaa tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg
     5521 gattattttc atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat
     5581 gaaattcctt tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg
     5641 ttgtgaagaa gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc
     5701 gaaaagagga gcagcgacaa taattttgtc gctgctcctt ttaaatttat tttgtcattc
     5761 ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac
     5821 agttgattgt ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc
     5881 agcttctttt aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc
     5941 taatccacta ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc
     6001 taactgttta tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg
     6061 ttcagtaata gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt
     6121 tggtgtaaca aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag
     6181 atcttgtcct aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg
     6241 caaggcatca acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat
     6301 atggatcata taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact
     6361 tgtttttcca actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc
     6421 accgtaaagg acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa
     6481 ttcttgtacg gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc
     6541 atttgtattg gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg
     6601 gttagataca gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac
     6661 ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac
     6721 atcataataa gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg
     6781 attccagaat gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact
     6841 taaaatatca ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc
     6901 ttgattcaag ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc
     6961 ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt
     7021 gatgaaaggt gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag
     7081 gaattgctgg aagctttcta aatggatata aatcacattt tttcctttag cgagcccgaa
     7141 caaatcgtca tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc
     7201 attgggactt gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac
     7261 catgaaagca ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag
     7321 caattctgga cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa
     7381 tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt
     7441 tgcaatcagc agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg
     7501 aaacagtcga atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt
     7561 gttgatcgta atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa
     7621 tgtcaaaaga aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata
     7681 aagagcaatt ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta
     7741 ttgaatgcca ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa
     7801 ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt
     7861 ttttatacat ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa
     7921 accatgttac acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa
     7981 ttgaaaccag aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga
     8041 gcagtaagac aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga
     8101 aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact
     8161 gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt
     8221 tgattcgcaa aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg
     8281 ttgggtattg agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt
     8341 gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat
     8401 attcaatgga gaaggagacg gaatcggcgg tctgattgtt gattattatg caggttatgc
     8461 tgttttttct tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt
     8521 tcgtgaaatc tacccggaaa tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa
     8581 tctgcctgag agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga
     8641 aaacggagta aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga
     8701 ccaaaaagaa gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa
     8761 catgtttagt tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac
     8821 aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa
     8881 tggtttggat gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata
     8941 cgccaaaaga aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag
     9001 aaataagaaa aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct
     9061 ggatatttta gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat
     9121 agaacaattc caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca
     9181 acttgcttta tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa
     9241 ttatctgaaa gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat
     9301 ggtttgggta aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat
     9361 cggggccgtc cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga
     9421 tttagtcgaa tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga
     9481 aacattggtt cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac
     9541 cttagctgaa ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca
     9601 tgtgatacac acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct
     9661 aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca
     9721 tgattttaaa gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca
     9781 ttttggagcc gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg
     9841 gttgatggag ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc
     9901 catgggaaat cttggtaaaa tttctcgtct ggctggcgga acgacgggtt ccgttatgac
     9961 atttggttcc ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt
    10021 gaagcaaatt attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt
    10081 tggcaaagaa aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc
    10141 cctatacaga gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg
    10201 aacagcttcc agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga
    10261 cggggcctct tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa
    10321 aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg
    10381 gatacatacg tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag
    10441 atcaaattgc agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga
    10501 tggagttagc acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac
    10561 aataaatcat acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc
    10621 tttctttttt atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc
    10681 gaaagccgct gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc
    10741 aaaatacaga ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga
    10801 aacccctccg ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt
    10861 aaactctttg catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt
    10921 cagatgatcc atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc
    10981 atgccaagca ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat
    11041 aagctcttgt ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt
    11101 tttctctaat tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc
    11161 gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa
    11221 aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg
    11281 ttcatccgat atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg
    11341 cttcacgatc ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt
    11401 tgttaagata atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt
    11461 atgttctttg tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc
    11521 tttcagacga ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat
    11581 ccgtccatga tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc
    11641 gaaattattc cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc
    11701 aatcaaatca tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag
    11761 tagttttttt cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc
    11821 tgcactgatc gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag
    11881 tttttccatt ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa
    11941 aaatgttttg agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt
    12001 ggaacgatga caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag
    12061 gatcaagggc aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct
    12121 tttttggaca ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca
    12181 gaaagagtta ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat
    12241 agagggtacc gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc
    12301 catcctagtg tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat
    12361 agtttaaccg gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga
    12421 aaatctgata atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat
    12481 atcggcccat cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc
    12541 tatttgttcg gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa
    12601 cctttggatt tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat
    12661 tatgtcaaac atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt
    12721 tccggaagcg ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa
    12781 gttgttacat gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa
    12841 ctattgaaag aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt
    12901 ccttctgcta tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc
    12961 gctttgccat gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa
    13021 aatgggatca aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct
    13081 gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta
    13141 gcagtatctg ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa
    13201 gtggatgagc agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca
    13261 gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa
    13321 gttgccaaag caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat
    13381 tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt
    13441 agagtaaacc tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta
    13501 gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac
    13561 aacgaattac gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca
    13621 acgaactatg acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct
    13681 gattctgaag tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg
    13741 atcgacttct tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca
    13801 caaatcttct ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta
    13861 atcggtgatc gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca
    13921 gcgattgcgt tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca
    13981 aacaaacgta tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat
    14041 gcagaaggtt gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta
    14101 actgctgttg gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa
    14161 ggtgccgctg atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac
    14221 caatacgcag caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc
    14281 aactatgaac caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct
    14341 gaaggaaaag accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac
    14401 tctttaggac aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt
    14461 gaaaaacctc gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc
    14521 tacttgcaag gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt
    14581 gctcatacag acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat
    14641 acattgggtt atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat
    14701 ggaatcaacc cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt
    14761 ctaggcaaac caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag
    14821 ggtttttcgc tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact
    14881 ggtacccatt taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca
    14941 agacgtttgt cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt
    15001 tgttttagga ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt
    15061 tctagaatca gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag
    15121 cttaagatag gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt
    15181 ttttctctat gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag
    15241 cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac
    15301 cgttttcagc ataagcatct acgttgtatt taaaacctct tccaattact tccccattat
    15361 ctgctttgat atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac
    15421 ctacataagt agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga
    15481 taacgatcaa ccctaaaatt tttttcatga aagatctcct tttttatttt tattataccc
    15541 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
    15601 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
    15661 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaaaaaacag
    15721 tctcaactgt
//
LOCUS       DAZBTH010000060        15329 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000060 DAZBTH010000000
VERSION     DAZBTH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.061"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..438
                     /locus_tag="ACHGNW_001810"
     CDS             88..438
                     /locus_tag="ACHGNW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN6926098.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            537..1067
                     /locus_tag="ACHGNW_001811"
     CDS             537..1067
                     /locus_tag="ACHGNW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6926099.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1221..2372
                     /locus_tag="ACHGNW_001812"
     CDS             1221..2372
                     /locus_tag="ACHGNW_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6926100.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2481..4520)
                     /locus_tag="ACHGNW_001813"
     CDS             complement(2481..4520)
                     /locus_tag="ACHGNW_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6926101.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4578..5135)
                     /locus_tag="ACHGNW_001814"
                     /pseudo
     CDS             complement(4578..5135)
                     /locus_tag="ACHGNW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5283..5449)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5674..5880)
                     /locus_tag="ACHGNW_001815"
     CDS             complement(5674..5880)
                     /locus_tag="ACHGNW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926102.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5896..6510)
                     /locus_tag="ACHGNW_001816"
     CDS             complement(5896..6510)
                     /locus_tag="ACHGNW_001816"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN6926103.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6657..7340)
                     /locus_tag="ACHGNW_001817"
     CDS             complement(6657..7340)
                     /locus_tag="ACHGNW_001817"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6926104.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7449..7907)
                     /locus_tag="ACHGNW_001818"
     CDS             complement(7449..7907)
                     /locus_tag="ACHGNW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN6926105.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7991..9754)
                     /locus_tag="ACHGNW_001819"
     CDS             complement(7991..9754)
                     /locus_tag="ACHGNW_001819"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN6926106.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9975..11435)
                     /locus_tag="ACHGNW_001820"
     CDS             complement(9975..11435)
                     /locus_tag="ACHGNW_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926107.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="ACHGNW_001821"
     CDS             complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="ACHGNW_001821"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN6926108.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12592..14019)
                     /locus_tag="ACHGNW_001822"
     CDS             complement(12592..14019)
                     /locus_tag="ACHGNW_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6926109.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14050..15264)
                     /locus_tag="ACHGNW_001823"
     CDS             complement(14050..15264)
                     /locus_tag="ACHGNW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN6926110.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4373 a         3074 c         2644 g         5238 t
ORIGIN      
        1 ttgttttttt gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata
       61 atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt
      121 gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa
      181 gtagatgaaa tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta
      241 caaaaacaag tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt
      301 tccttgatta caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg
      361 aaagaaattc gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa
      421 aagatcctca gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat
      481 ttaatttggt aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt
      541 ttgagacgat taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta
      601 tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga
      661 tcaccgccta tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa
      721 ggaaaacagg ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc
      781 tgatcgtcta tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag
      841 ctgttatcat caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt
      901 ggagtgttta tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat
      961 tcaaaagttt gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc
     1021 aggagattat caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta
     1081 ttaaaataga ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt
     1141 tgtgagtata ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt
     1201 tatacaaagg aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa
     1261 caacgattgg ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac
     1321 ttgttggtgt tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc
     1381 atgacttttc ggagattggt gtgatcttat taatgttttt agctggtctg gaaagtgatt
     1441 tatctctttt gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct
     1501 tcccggttgt ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga
     1561 ttttctttgg gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag
     1621 aactaaacgt agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg
     1681 acattttagt cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta
     1741 gcaatgcttc tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct
     1801 tattagtcaa atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt
     1861 cttctattat catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg
     1921 ttgggcttag ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg
     1981 ttaaagaaga agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat
     2041 tttttgtcag tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta
     2101 tttctatatt gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc
     2161 agattgccgg tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg
     2221 gagaaatggc tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt
     2281 actattcacc tttagttatc gtgattttat taagtacact tatttctcca cttgttctca
     2341 aatattttac aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga
     2401 catcgtgaaa accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac
     2461 tgtacttaaa aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat
     2521 gctcgtttgg ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa
     2581 ccttggttat caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt
     2641 ttttctttga tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga
     2701 gaatgtgctg taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt
     2761 actgctgttt cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta
     2821 ggatagacaa gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga
     2881 gtaattagaa gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag
     2941 ctatcttcat tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca
     3001 aaaatgaatt tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt
     3061 gccgcatata tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca
     3121 ctactgacac gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg
     3181 ttgatcgtca acacttcatt tggatcgata gtgccgttgt cgagaccgat tgctgctgta
     3241 atcattttaa acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt
     3301 tcaggatttt cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt
     3361 ggatcatagc ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca
     3421 acagttgatg cagcttttcc tcctagactg tcaaaagcta ttttttgtgc cttcgcatcg
     3481 attgtcaatt tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct
     3541 acaccgtctg catctgtaat gcttaatttc ccacctgttg tcccacgaag atccttatca
     3601 aaagccatct ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta
     3661 tcaatatctt ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc
     3721 aaaggataat atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt
     3781 gctccatcaa tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc
     3841 gcctgattga tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct
     3901 gttttttcgc ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct
     3961 gttgttgcta atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg
     4021 gtcaggctta ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg
     4081 atcgtggttt gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca
     4141 cctaatgagg tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta
     4201 tctactttta gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg
     4261 tatttatcta agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc
     4321 atttctgcag ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc
     4381 ttttctccag cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca
     4441 cccgcagctg ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg
     4501 tgcttgtcac ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact
     4561 atttatacct gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc
     4621 acactttctg gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc
     4681 ttgaatattg aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc
     4741 cttctgatcc tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa
     4801 gtattaccat ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat
     4861 ttttctgcat cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct
     4921 tgaaatgtga tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga
     4981 tttaataaat caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct
     5041 ggaatatcga catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg
     5101 ttgtattgtg caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg
     5161 catctcctcc tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat
     5221 aagtcagtca caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc
     5281 tagcatcatc tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct
     5341 attggaaaaa gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc
     5401 tgggcaacag cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat
     5461 ttacgagttc atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt
     5521 gaaatttaac aatctttaat attttttaga aaaaaataac aaataagttg aaagaatccc
     5581 gttgcaaatt ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt
     5641 tttatatgtt ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc
     5701 gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac
     5761 aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt
     5821 ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat
     5881 aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata
     5941 atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt
     6001 atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc
     6061 tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac
     6121 ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt
     6181 caggtacagt tcgctttcct cattaggcag attggctgct gttttattta cttcttctac
     6241 gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt
     6301 gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact
     6361 gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt
     6421 tactactgct actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc
     6481 tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta
     6541 aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc
     6601 tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag
     6661 ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata
     6721 atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga
     6781 gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat
     6841 aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta
     6901 gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct
     6961 gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct
     7021 gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt
     7081 tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg
     7141 ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta
     7201 tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct ccccataata
     7261 aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg
     7321 atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca
     7381 tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac
     7441 ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta
     7501 aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc
     7561 caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca
     7621 ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc attttttcca
     7681 ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga
     7741 tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct
     7801 gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga
     7861 tatttttctt ggaacttttt gacatattct tctacttgct gatacataac atccacccct
     7921 ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga
     7981 gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg
     8041 ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg
     8101 caccaaaatt taatacgttc attgaagaat caccataata acgactgata ttttcgccaa
     8161 atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt
     8221 tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga
     8281 ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag
     8341 ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat
     8401 ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg
     8461 ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa
     8521 gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc
     8581 ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg
     8641 ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga
     8701 ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga
     8761 tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc
     8821 ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca
     8881 aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg
     8941 tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat
     9001 aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt
     9061 cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa
     9121 atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta
     9181 agtttttata taaactgcca tgattctcat caatagacat ctcattatcg tttactaata
     9241 cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac
     9301 ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg
     9361 ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt
     9421 catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa
     9481 aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa
     9541 cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac
     9601 ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca
     9661 tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat
     9721 tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct
     9781 tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga gttgactcta
     9841 agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac
     9901 gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc
     9961 taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga
    10021 tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct
    10081 aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat
    10141 tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct
    10201 tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca
    10261 acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg
    10321 acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac
    10381 tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag
    10441 atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag
    10501 gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa
    10561 atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct
    10621 tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata
    10681 taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag
    10741 actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg
    10801 ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct
    10861 tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct
    10921 tcttctacat acggattttt attttcatag ccttcccaat tgacataacg tactaaagct
    10981 agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca
    11041 ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata
    11101 aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac
    11161 tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt
    11221 ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca atattctaga
    11281 cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata
    11341 gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt
    11401 ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc
    11461 ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa
    11521 gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt
    11581 cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat
    11641 ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca
    11701 gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt
    11761 ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg
    11821 gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta
    11881 gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc
    11941 acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa
    12001 gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca
    12061 agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg
    12121 atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct
    12181 gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc
    12241 attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt
    12301 ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac
    12361 aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca
    12421 atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca
    12481 gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca
    12541 atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc
    12601 tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag
    12661 ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg
    12721 tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat
    12781 tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa
    12841 aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt
    12901 caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc
    12961 tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt
    13021 gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt
    13081 tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc
    13141 aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga
    13201 ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa
    13261 tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata
    13321 atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata
    13381 tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa
    13441 tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa
    13501 gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa
    13561 tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag
    13621 gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa
    13681 tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa
    13741 aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc
    13801 gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
    13861 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
    13921 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
    13981 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
    14041 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
    14101 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
    14161 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
    14221 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
    14281 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
    14341 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
    14401 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
    14461 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
    14521 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
    14581 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
    14641 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
    14701 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
    14761 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
    14821 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
    14881 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
    14941 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
    15001 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
    15061 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
    15121 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
    15181 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
    15241 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
    15301 gaaggtgtag ttgttttttt cttctgttt
//
LOCUS       DAZBTH010000061        15301 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000061 DAZBTH010000000
VERSION     DAZBTH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.062"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1152)
                     /locus_tag="ACHGNW_001824"
     CDS             complement(<1..1152)
                     /locus_tag="ACHGNW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFN6926111.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEG"
     gene            complement(2024..3655)
                     /locus_tag="ACHGNW_001825"
     CDS             complement(2024..3655)
                     /locus_tag="ACHGNW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN6926112.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3669..5342)
                     /locus_tag="ACHGNW_001826"
     CDS             complement(3669..5342)
                     /locus_tag="ACHGNW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN6926113.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5355..5672)
                     /locus_tag="ACHGNW_001827"
     CDS             complement(5355..5672)
                     /locus_tag="ACHGNW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6926114.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5697..7097)
                     /gene="lacG"
                     /locus_tag="ACHGNW_001828"
     CDS             complement(5697..7097)
                     /gene="lacG"
                     /locus_tag="ACHGNW_001828"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN6926115.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7334..8119
                     /locus_tag="ACHGNW_001829"
     CDS             7334..8119
                     /locus_tag="ACHGNW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6926116.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8122..8814
                     /locus_tag="ACHGNW_001830"
     CDS             8122..8814
                     /locus_tag="ACHGNW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6926117.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9088..9459)
                     /locus_tag="ACHGNW_001831"
     CDS             complement(9088..9459)
                     /locus_tag="ACHGNW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN6926118.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(9859..10089)
                     /locus_tag="ACHGNW_001832"
     CDS             complement(9859..10089)
                     /locus_tag="ACHGNW_001832"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN6926119.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(10183..10521)
                     /locus_tag="ACHGNW_001833"
     CDS             complement(10183..10521)
                     /locus_tag="ACHGNW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926120.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(11449..11565)
                     /locus_tag="ACHGNW_001834"
     CDS             complement(11449..11565)
                     /locus_tag="ACHGNW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6926121.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(12041..12919)
                     /locus_tag="ACHGNW_001835"
     CDS             complement(12041..12919)
                     /locus_tag="ACHGNW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFN6926122.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(13242..14267)
                     /locus_tag="ACHGNW_001836"
     CDS             complement(13242..14267)
                     /locus_tag="ACHGNW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6926123.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            14626..15129
                     /locus_tag="ACHGNW_001837"
     CDS             14626..15129
                     /locus_tag="ACHGNW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6926124.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT         4782 a         3152 c         2540 g         4827 t
ORIGIN      
        1 gccttcggcc tgttggaaac ctttatgact gcgacctagg aatttttggt tgtaagtatc
       61 aaaagacgag actaagaaac gttccatcga ctcttcattt tggaactgtt ccttgcggtg
      121 gctgtatttc ttgatttgtt tattaaagga ttcaatcaag ttcgtagaat aaatactctt
      181 gcgtatggcc aaggggaaat catagaaagt gagcaagtga tcgttcgaaa gaatcgattc
      241 aaccactttt ggatagctgg ttttccattt ttccgcaaaa gtaccacgtg cttccaacgc
      301 cacctcttta gatgaggctt gatacaccat tttaaaatca tcacagactt ccttacgatc
      361 atcgacacgc actttatgac tgatattacg ggaaacgtgt acacaacaat gttgaaaacg
      421 agctttggga tagaaccgac taatcgctcc taccatcccc tttaagccat ccgtgatgaa
      481 caggaggaca tttttcaaac cgcgctcttg aaggtccaat aaaatttcct cccaaatcgt
      541 gatggattca gtcggtgcaa tcgcatagct caatacttcc tttgatccgt ccgggcgaat
      601 gccaactgcg atatgaatag cttccttggc gacggttttc cgctttaacg gaatatacgt
      661 tgcgtccata taaatagcag cataacggtc atgaagctcc cgcccattaa acgccgttac
      721 ctcttctgta aatgattttg ttatattgga catggtttgg ggcgtgtagt aatgcccata
      781 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
      841 gacggtctcc tctaacgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa
      901 ctcaccattg cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt
      961 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat catacttttc
     1021 gtaatctagg aaagccgtta actccgtttt gagaagcgta ttgactgctt tctccaaatg
     1081 gacacggaat aattcattca aatcgccttt gttcgctaga gtctttagaa tttctgtagt
     1141 aaaatcgttc atagggaagt cctctcttct gtgaatgtgt tgtggtaact ctattctaca
     1201 gaagggactt ccttttttct atttacacaa aatattttac actctccatt ataacaattg
     1261 ggttaaaaag aaaatagata gtattctacc tcatatttat ttgaaagtaa tatataaaag
     1321 acgttcaaat tcgaacgcct ctcgttaacc aaatattgga ttcagaatct agttttcata
     1381 tataaacaga gtatgattag gtgcaaaaat tgcactttta ttgcactttt atttttattt
     1441 atagtgtttt actgttctgt atattgtttt aactcccttg atagtatcgc attctgtttt
     1501 atacacttta cgttgaaaac agagtgggaa taaagagcca acataatatg gagggggaat
     1561 ccctataaat caacattctt caatagctaa aatgcttgat tttcattaaa aataagatgg
     1621 ttccctacca aaagagacta taatataagt taatttgttt cattcttatc ataaaaagaa
     1681 gttctcgaaa aacgaactgt tttccgagaa cttttttcta tccatctaag tgctcaaatc
     1741 atattcacga cggaattcta aggttaccct ttcttaaaca aactttatgt tatctgcaca
     1801 actttttgca attttttcat tgcttcttac gaaatcgttg aactctcttt agcataatac
     1861 aaagtgtaga tgaagtctgg tgctttactc gttttggtaa aaatgactgg aaatcgtttc
     1921 tggttatttt tggcaaaggc gaggtgcagg ttcgattcct gccttctacg ctaacgatgt
     1981 ggctgaaggg aacctccgat cttgaatcga aacgatccgc agtctattgt ttcaccaatg
     2041 aaacaaaatc aacgttcgtc caagcatttc ctgaagcctt gctataaacc ccaatttcac
     2101 aaacacctgt tgtaattgga atgtctttga ttgtaatttt tgtccattca cttgttttaa
     2161 caaccgcagt atttagttcc gctccaccat aatttttgat atataactga ttagtatcaa
     2221 actctccccc agacattaca taggcactca atgtgtaagt tcctttgctt atgccactaa
     2281 cattttgata agctgatgcc tcgtatgctt gatcctgcca aaaagttagt ttgtagtccc
     2341 caaaaaaagt gcccgcttcc gttttgatcg tacccttagc ggttccttgt tgaaaccagg
     2401 tttgccaatt ggaaggactg gtcgttggtc catcgctttc gaaacttgca tttttaacta
     2461 gatttatttg cgcccctgct tgtttaaacg cacgcaagct tggcaaggcg tttccagcaa
     2521 aatcaaacat cgcttggttg tcccattcgt ttcccatagt ggattgaaca ctgagataat
     2581 tcatacctgc ttgtgttgcc caactcacat ttccgttgta ccaaagaggt tcccaataga
     2641 agaagcccgc cccgcgatta ttcggtactg cctgaatccg atcggcaaga tccagcaaat
     2701 actctgcttg tcctgtcggt gttggttcat atccccccgc agctgcttcg gcttcaccaa
     2761 aagcatttgg catagtatcg gcattcgctg ttgtatgccc ataagctgtt tcaactacga
     2821 tcacttcttt accataccgt tggctgatcg cattcatatt ggtttgaagt tcattcattg
     2881 aaccgtgcca atacggataa tacgacactc caattaaatc aaatgacaca cctcgactcg
     2941 tgatttcatc aaaccaccaa gtaaatgctc ctgtatcgcc accatcagct aagtggagca
     3001 ttactggagt tgttttaggt tgagtgtctc gaacagcttg gataccagac ttcaaaaaag
     3061 cagccaatcg atcaaattct cccccgcctt ctccccaaga cttgccataa ggccagagca
     3121 ttccgctatt cagttcattg ccgatctgaa ccatatcagg ataaacgtcc agctgcttca
     3181 tttccgacag aacgttcttt gtatagtcgt acaatgccgt attcatttct tcaaaggtca
     3241 gaccttgcca tgctttagga agattctgtt tcccaggatc aacccaaaaa tcactgtagt
     3301 gaaaatccag cagcacctta agtcccagat tttttgctcg ttttgcaagg tcaatcgtcg
     3361 tttccagatc atttgtccct gctccataac tttctccatt actattataa ggatcctgcc
     3421 acaaacgcag tcgaacatag ttgacaccat tattttttaa aatctctaag gcatcttttg
     3481 aaatatcgtc ttcataatat tttgcaccag atttttccat atctgacagg atcgaaatat
     3541 ctgctccgcg aataaattcg ctggcagatg cttcaacagt ttttgaaaca gcccccaaag
     3601 cccctaacac agctgcaaat aaaacagctt tacgcaacca aattcccatt ttcattatgg
     3661 ctcctccttt attgaaattg gttcagaata aactgaacag ctttttcagg atctctcgtt
     3721 aatccgatat attctgctcc ttttgtcgca accagcttga ttcctaagcg atcggtgtct
     3781 tctttgatat catcatagta agaattgact tgcggtgcta agaccaccat atcaaaatcc
     3841 tgcagcattt cgtaatgaga accataagca cccgccattg cactgatcgg tgctttcgat
     3901 tcagatgcac ctttttttaa agcattagct aacatcgcac ttgtccctgc gccagcacaa
     3961 agtaccaaca cattgatttc tttatttgca tcgctgataa cttgactcgc tgccacaggt
     4021 acttcctcac gtacatcggc agaagcacct tcctcggttg cgatcacctc tgctttttgt
     4081 gcttctttct ctactaagac agcatcgtat gctttgcaaa atggtagata gatcaggaag
     4141 tcaacgacga ttagaaccgc tgctagaaca aaagctaaga tacccatgcc cgttcctaga
     4201 accaaaccga tcggtgctgg agttgcccaa ggcaaaacgt acataaagct gttcatgccc
     4261 aatacatcga caaaaaactt gaacaggcaa acgtttacaa caggtgttaa caaaaatgga
     4321 ataaaaaaat aagggttcaa aatcatgggt gccgcaaata gcaatggttc gttgacagcg
     4381 aaacttactg gaataaagga agctttccca actgccttca actgcttaga cttagcaaag
     4441 agtaggaaga taaaaggaac aaccaatgtt gccccagttc ctcccatagt tcctacaaaa
     4501 ttccctaaac ccaccgtcag tactttagaa gcttgttcac cggaattgaa caattgcaga
     4561 ttggtttcca cattggcata gataattgcc gcaattgccg gctccacgat cgacggtcca
     4621 tgaacaccga caaaccaaaa cagcgccatc gctccccaga taatagcgat tcctaagtac
     4681 ccgtcagctg ctgtaaatag cggctgcagc accgtaatga ttgcttctgc aaatgagatt
     4741 tggaagattt gtcgaatgcc taaatcaatt aaaacggccg caaatactga aaaagagaaa
     4801 ggaaaaatat ctcggaaggt ttgcgaaatc gttccaggaa cttctttagg cattttaatg
     4861 gtaatatctt tcagcacaca aaacttatat aaattaactg ttaaaaatgc agcgacaaag
     4921 gaagccagta gtccctttgt ccccatatat tccgttgaaa atccaccttc aatcggagag
     4981 acgcttaaca acaggaaact taccacagag gccagcatca cagaggtggt gttgattact
     5041 ttgtttttcg gtaagcgacg attcatcgaa tcagccaagc agcgagcagt ggttgctgaa
     5101 acgagtaacc ctacaacacc catcgaataa ttatagactt tccatagcca tgtggagaca
     5161 gattcaggcc attggtaacc aaaaatctca ggaaccgcag caatcaaaat gaaaatgctt
     5221 gaaaataaaa tagctggcat cgctgttaaa aatccgtcac gaacggctcc taaataacta
     5281 ttacgtgaga ttttttcaaa aaatggtttc cctttttcga tttgtttaat gattccatcc
     5341 atagcgttct tctcctatcc ttgattctgt ctgtacagta caataaagtc ttgaatcaga
     5401 tctctcagca ataaagtggt cattaaatga tcctgcccat ggataaaaat gaatcccatc
     5461 tccatctctt tcccagatgc ttcttccgct aaaatttttg tttgcgaatt atgtgcaagg
     5521 gttaaatttt cgtcggcttc tttcaacgcc gaatcaacat gttcaaagtt tccttgtctt
     5581 acttctttca ataatttcaa taatgtactg cgagcgtcgc ctgaatatgc aacgatctca
     5641 aaaccaagca tttgtaattc ctctttattc atcttttctt cctccattat cacttattaa
     5701 ataatcgttt tggtttcact gaccaacttg taccagtaag ctgattcttt tgggtaacgc
     5761 ttctgggttt caaaatcaac ataaaacaag ccataccttt tgttataccc atttgtccat
     5821 gagaaaagat ccataagcga ccacaaaaaa taccccttca catttacccc ggctgttatc
     5881 gccttgctta aggattccaa atagactctc aaatagtcga ttctcggctg atccataata
     5941 atgccatctt caaactgatc tttatacccc atcccgttct cagtgatata gattttgttg
     6001 taatgtgggt aatcgctttt aattcgcagc aacaagtcat atagaccttc tggataaatc
     6061 agccagtccc aatcagttcg aggaattcct tctttataaa tacgttctcc gattcccttc
     6121 actttataaa cagaggtccc tttttcaccc gtaccgttat gatggattgc attttctcca
     6181 tcgtaagctt tgacaaaatg gcattggtag tgattgatcc ctaagtagtc attacgcgtg
     6241 gatgcttttt tcaattcaac gaaatcttct tcaggaaaat gatagctggc ctgatttgct
     6301 tcacatatct catcaagagc agtcaaggtt tcagttgaat aatagcctaa ataggtagca
     6361 tctaataaga aacggatcga caatgcatcg tctaaaaagg cagcatgctt atcttccggc
     6421 gcgtctgttg ccgcatattt cgtctctaat gagtgaacta caccaatttc acccggaagt
     6481 tcgttttctt tgaaatagtt cactacacgg gcatgagcaa ccatcatatt atgaagacag
     6541 gcgacgatct ttgtaaaatc atatttgatt cctggtggga aaacacctaa caaatattga
     6601 ttggttgcaa ctggatagat ttcattaaag gtactccaca ccttgacttc tttgaattca
     6661 tgaaaacaaa aaatggcata ggaaacaaat gcttctattg tttctcgatt caaaaaatct
     6721 ccatggtcaa acaaacgttt aggggtatca aaatgatgca acgtcacaaa cggcgttacg
     6781 tttcttttct tacattcttc gaacacacga tgataaaaag cgattccttc tggatttggt
     6841 ttcccagctc catcaggaaa aatgcggctc caagcaatgg ataatcttag accatttaca
     6901 ccaaaacgtt cacacaattc aatgtccttt gcatattgat gataaaaatc acttgccgga
     6961 tcgggactga atcgcccttg ttcctctaaa aagtcatccc aagcaacagc gcctttgcca
     7021 ccttccttag tggctccttc aacctgatag gctgctgtgg ctccgccaaa aataaaatct
     7081 tctggtaatt taagcatgta catctctcct caaatctata atcagttttc gctctttcta
     7141 actgattgca ttgtaaacgc atccaaaaca tttgtcaaca aaaacaaaca caaataatca
     7201 taaaaaccag aaaacagcaa taaaatctat tgtaacaaac ataaaaacca ccctaaatgt
     7261 ttacttttgt tcgaattaga ttgactgttg tccgtttata tgatagatta tccttaaatt
     7321 agaggtgaag acaatgttga aaaaagaaag attgcttact atcgtagaaa tggtcaataa
     7381 aaaaggaatc cttaccgtta atgaaatcat caatcaatta gatgtttctg atatgaccgt
     7441 tcgcagagat ttagatgaat tagaaaaatc gggcaagctg ctgcgtgtcc atggaggggc
     7501 tcaaagtctt tcctacactt tagatcaaga actttctcac actgaaaaag ccactctgca
     7561 gattcaagaa aaaaaagaaa ttgctgccac agcagcctcc ttgattcacg agggagaaac
     7621 gattttctta ggaccaggta caacaatcga actattggcg acgcatttac tgaacaagaa
     7681 aattcggatc attacaaata attatccagt ttttgatatt cttgctcaag cagatggacc
     7741 agaaattcta ctgattggcg gtgatttccg gaaaaatacc ggcgcatttg tcggaccgat
     7801 caccaacgac aatttaagaa gactaaagtt tacaaaagca tttatcagtt caaatggcgt
     7861 tcacaacgaa gcgatctcta cttacagtac cgaagaagga gaagcacagc agatcgctct
     7921 aaataattcc cgtaagaaat atctactcgt tgataataaa aaattcaatc gagatgattt
     7981 ttatgtgttt tataatttgc atgatttcga tctgctcatt acggatcgcc agatctcgcg
     8041 ggaggttcaa gagcactatg cacaatatgt cgatctgcaa attgctgatt caaccgaaaa
     8101 taccactata ggagcgtaac tatgaaaggg attatatttg atttcaacgg aacaatgttt
     8161 ttggactctc atctgcatga agctgcttgg ctgcatatga tacatgatca tacaaaagat
     8221 ggtttatcag acgaggatat tttaaaaaat attcatggtc ggacgaatac ggagattttg
     8281 aaacatttca tttcagagca tttgaccgcc tctgagatcg ccgtattgtc agaagaaaaa
     8341 gaaagctatt atcgtatgct ttgcttgcaa aatgaaaacg agcttcagtt gacaaaaggg
     8401 cttgaagcga ctcttgacca gctgactgac caacagattc cattaacgat tgccacggct
     8461 actgtcaaag agaatgttgc tttttatttt gatattttcg atttagcccg ctggtttgac
     8521 tgggacaaga ttgtctatga cgacggctct ttccctggga aaccgcaacc ggatattttt
     8581 cttaaagcgg cagacaaact gagcttagct ccggctgatt gtcttgttat cgaagatgct
     8641 tactcaggat tacttgctgc caaacgtgcg gggatcggaa caattattgc aattgatcca
     8701 ttcggtaaaa acaaaacagt atttgaacag gcacagctaa ataaagatgg aaccatcaaa
     8761 gattttactg gtttttggac agagcacctg tctgccacat ccgttaattc ttaaacgatt
     8821 caactaacta ccaaagaaaa gcaactgaaa tgcacacctc cttttataaa aaactgccag
     8881 tgaaacgaca tgaaagatac actgctatat ccttagtgaa atatagcagt gtatctttga
     8941 ctttttcatt cttttaacca tatgacgcgt tcatctctat ctataattaa cattaaaaat
     9001 agtgttttca cattatttct caaaccaatc ttaaaaatca tcaggagtac ttttttgata
     9061 tacttttatt aatgctgggc gtaatggtta aaagatttcc actagttcct ctccaccaag
     9121 ctgagttgaa aaatgcttat ctgtaaacaa catagacaaa gaggaaataa acgtttccgt
     9181 atctttctct gtcccaagga tattaattaa ttttcctact aggtaagtat cattaaactt
     9241 aagatctgcc ttcacatcaa aagttttgat aaaagctttt atcatgcgta caattttgtc
     9301 tttatcgcca taattgattt caccactcat atcaatatag tcactaatcc ctgaagaaat
     9361 aatggcaact gcttcttctt tcgccttatc agataattca tttaatgact gggattgatt
     9421 gatgacttct tgagttttat ctgttacgta atctctcatt atcctatcgg catactccgc
     9481 aaagtaatct tgaaaaaatt cttctacagg gtaaccaact tcgttttccc aaccaggaga
     9541 aatttcatta tcagtactta ttaaatctat taaaaaccaa cccgttctct gagtgttgat
     9601 tctataatat ttttaatttc ctatataaat caaaaatatt atagaatcaa taagctaatt
     9661 aagaaacaaa ctttagaaac tctagaagaa cttctgaatt tatatagaag tgtgtagcta
     9721 cttactaaat atacaaaagt taaaataaaa tactctggat actctttata ctgcattaaa
     9781 gttagtaata ttaacagcaa agatatcgaa aaaacaataa cataatatgt gtcaaaattt
     9841 ttcataataa gaactccctt actgttgcat actaacataa tccaaaaact cttctacttc
     9901 agctaaagac attccacttt ctttagtcca aaaaacagtt ttttctatcg atccttctct
     9961 gtaaactgta aatgtcggag tcgagttaat tgagtagcga ctcttgaatt ctttccactc
    10021 attttcatct tctctaactt ttgctacatt cacgtaataa atattttctt ttcccttttc
    10081 taccagcata tcgaatagct tcctgctttt gatgatcgat ttcttgtgct acatctagta
    10141 aattttcatc aatttctata tcataaagta tttcactagt gtctacatcc acaatatata
    10201 ctttttatca gtagcaattt ttgagtaatc cgttaaaatg atctcagtct cgtcactttc
    10261 atcagtataa atatacgtta acaatgaact caccttttca atcggtgtta gctctaagcc
    10321 tactgtattt tttactattg agtcattatt taccaccata acttcgtact gtatatacca
    10381 tcctatttgg tttccatcaa tatcagtaat tggctcacta gctaatggag tctccggaac
    10441 aatattttcc tgatatttct taaactcttc tgtagcacga aaaaggttat catgttcctc
    10501 acttgaaact ggttgagtca tagaaaatgt tacttggcta aacagaggga aaataaacac
    10561 tacaatcaac aaagtataaa aaaaagccct tctttttcat tttttatctt ccaatcacaa
    10621 attgaaaaac ttattcctgc aactttgagt ctagtatcac aatcgactga ctacaactat
    10681 tcatttacat tctactttaa gttcctcctt aaccattaat tttattaaag atacttgtaa
    10741 tagtgattct cacaaaattt catgaaaaat actttattac tgtatctaaa attccaatac
    10801 acaacttatc gactattatc tgaaaaccaa aacttattat tccttgatgt tattctagca
    10861 atagtaactg cttttttatt tttttgcata ttttttcggc gttacttttt aaagttaact
    10921 ttttcgacaa atagaaagag atacgaatca ctgatttttt tgaagaaaag aaatggagtg
    10981 agctgaacgt tagagatatt gggagctttc gtaactgctt attaaacgat aatggttatt
    11041 tgcaaccgta ttgctcgctt ctacatgaaa tatttcatga aatatttcgc aaaacctttg
    11101 attatgtagt tacgtttcca ttcttgacta aaaatgtttc aaaaacgaac aaaggtgtta
    11161 aaaggtaaag tgatcaatac tactggtcaa aataagaggt tgaaagggaa ttgaaaaata
    11221 actacagata atacttttat aaacgtacgt gctttgtggt tctgtagtta actatataac
    11281 tggaattagg gaatggtatc aatttgaaaa gaaagattct accaatttat catatgttaa
    11341 aaatggaaaa tcaacagcag ttctttcgga aaccttacac aaaaaccgat tattgaaaat
    11401 agccgaaaaa cgtttttcgg ctattttttt aattaagttt ccttcttctt accttttccc
    11461 actattccgt ttcacatctt tgatgatctt cagcgcacgt tttcgtgttt tgatatttgg
    11521 gcttttcaag cggcggtatg cacttgagat atctgttttt tccatatgga cgacccactt
    11581 tcttctttga ttgtagtcat gctaaaaaac acaaatcgaa acaattacga tttgtgtttt
    11641 aaatggtatc aaaagttaaa atgaaagtca acttgttaaa acagtcgatc agactagctg
    11701 attgactcct atctctggaa ataaattttc attattacgt tttagctacc taaaaaaggc
    11761 cctcttgtcc gttaacttta atatctattg ttatagctag tttacctatt gaacgtactg
    11821 tgtgttcgat agaacactaa aaaagaggct gggacagaag cgtttaactc caagaaataa
    11881 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga
    11941 gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
    12001 tttgtctcac gctcttttaa actatttcga agtactaaac ttatgaagta agaataccta
    12061 agccagagcc aatgtgctcg cctgcaagca attgctcttt tgcttgtcgc tgtaaactcg
    12121 tactttctgg ataatcatgt atttgggcat ctactcgtcc aaagatccaa gggactccac
    12181 tatcgtcctg ttcatcaact gataataatc gataatcggc tgcaaaaaga cagatttttg
    12241 actctttttt caaccattta gtaagattgg gtgatagtaa ccaagtatct gtgtacatca
    12301 cttggtaatg gtaatcagga aaataaagtt caaaaaaacg aatggcactt tcgtaactat
    12361 ctcttatctt attctgagat aaatctgcat cggaaggaat atgaagagaa aggcactgct
    12421 ttccttctaa tcttagatcc gtgtaccctt tcggaaaata tgtcatttcg tactccaatt
    12481 caccaatccg aaagatcaag ccactagtgt aatgccaaat ccaaaaacca cggtcaaagc
    12541 gatagcttcc tgttacttgg aaatgttcat gaataaatct agtaaaagca ttcattgttg
    12601 ccaaatagat agttcttgga atcttcaatg gttgataaat ttttttccaa ctttgagaag
    12661 ctgcatatag ataaatagct aatgctgcaa aaggttcctc ttcatagact ttgtctattt
    12721 tctttgcagt cgcctttgct ttattaggat ccattagttc atgactatat tgctcaaaca
    12781 cctcatcaag gatgtttttt gcttgtacaa tcatttgctc tataacgact ttgggaagag
    12841 ttaaagtttc tgccagaatt tggatttcat tgcttatttt cttcatcaaa tttccccttt
    12901 tcatatcttt gaacctcatt gtttaaatga ctgattatcc ggacaccata tggtataaac
    12961 atacttttat tgtacactaa ctataaaact taaactttct atgaagaaag aatatctgta
    13021 agactttcaa ggaaagcatt gaccatctca ggaaagttta ttcagttacc atcataaaca
    13081 aaattttttg atagaataaa tataaaattt ttttgaaagt tcctttccac atttttgtgt
    13141 gaatttgact taatgctgag aatctggctt ttgaacacct tgaattagag gttgtgacaa
    13201 aaatcttgtc ataacctctt ttggcacata tatttgtcct tctattctaa tttttgacga
    13261 acaaccgtta aataatgttg caaaccaacc gtcattaccc acgcaacacc taaaccgatg
    13321 aaacttccta tgacgatccc taaaaaacga tattcgatca aagaaatcaa gtatgctcca
    13381 cgaataagtg tagatagcat caacgccatc ggattaagaa aaatattagc aactgcataa
    13441 tttctagcaa ctagaaattg tgcgattcca tatagaaaag tgatcagaaa aatggtctgc
    13501 aactgcgtaa acggcacaaa tgacaagaat gccgacacac ataacccacc catcgatcca
    13561 atcagatatt ggacttgtcg atgcttcatc gcatccaagt tttctgctag caaaatcgat
    13621 gcacatgaca aggtcaacca ataaggatta tgcagattca agccgtgact gacataaacg
    13681 gaaagaaata aggcagcgct gtagaaaaca gaatcaatga tcactaaagg ctcttcgtga
    13741 aatctttctt ttaaagttgc tttttcaatt gtctgctcag gccgtgaatc cagtaatttt
    13801 gtaatcactg ccgcaatcag ggcaaaaacg acacccataa aaaaataaag tgagacgatc
    13861 ggtaattgag cgattggcag ctgcatgctc gttcccatgg cagaaagcat cgcaaaaaac
    13921 aagccacctg gtttagaaat atggaataat cgcaacacga atctgctgga aaaagcaacc
    13981 aaagctacgg caaatggttc aagccataaa acataagtcg ataagatccc caaaacgaaa
    14041 gcaaccaaca gtccgcaacc gatcagaaac atacgtttca gcaaatattt catcggtatg
    14101 ttttgataat agaaaaaagt aaagatccct aatgatgaaa aacttgaaat caaaaaatca
    14161 tgatttatgt aacctaacca taatacaata aagataagta gcatcagatt catgatacga
    14221 aaaggattat cttgtatttt attgaaacga aaataagtct tgaacaatct atccctcctt
    14281 cctagtaatt ggaaaaaaag ctgaaccaag atgaaatgcc aatcccgttt gtcagcatta
    14341 cctagtataa caactatttc ttgatttttc ataaacttag tatcaaaaag taaacaactt
    14401 ggagaaatta gaacaaaaaa cgaacattcc gagtcgtttt gacagaaaaa agatagcaac
    14461 ttttccaagc aactcgctat cttagctttg ttaaagaaac cggattccca acaaaaaaaa
    14521 gcttctaatt ttcgtacgat aactacgttg gtccttaaac ttcatacttt tttatgattt
    14581 ttcagcggtt ttcgatggta tactttttaa atggaggaga tgtctatgcg gtatgtatca
    14641 aaatttttat tagggattta tttattcttt ctattatggt tgattttatt caagctatca
    14701 tttaatcttc cacaatttct cacttattct tacagcaatg tcaatctcgt ccctttttct
    14761 actttttctt ttgaaaatac gacggtatta cgtgagacca cttataactt gattgtcttt
    14821 tttccttttg gggtgctttt gaatgtcaat ttcaagagac tttctttttc taaaaaatta
    14881 gggattattt tccttgtaag cttcttggca gaattgattc aattcctatt cggtattggc
    14941 gtagcggaca tcactgattt gataactaat acaacaggag gactgatagg tttatgggcc
    15001 tatcaacttc taaacaaaca tctatcgaca aataagttag accgtctagc cattattttg
    15061 ggatacattt tattcttatt tctgatcgta gcttttttat ttttgacccg aactgtccgt
    15121 cttcaataaa atcgttcgta aaaaaagacc gtaatagaca cattccaatg tgtatctatg
    15181 acggtctttt tattcagtca ttggtttaaa aaaatctatt ttcatctcta atctactata
    15241 ttattgcagc aggacgactt gcagtttgaa actgacaggc tatattcccc ttatttaaca
    15301 a
//
LOCUS       DAZBTH010000062        14859 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000062 DAZBTH010000000
VERSION     DAZBTH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.063"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(340..1068)
                     /locus_tag="ACHGNW_001838"
     CDS             complement(340..1068)
                     /locus_tag="ACHGNW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926125.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1046..2620)
                     /locus_tag="ACHGNW_001839"
     CDS             complement(1046..2620)
                     /locus_tag="ACHGNW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6926126.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2849..3787
                     /locus_tag="ACHGNW_001840"
     CDS             2849..3787
                     /locus_tag="ACHGNW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926127.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3799..4710
                     /locus_tag="ACHGNW_001841"
     CDS             3799..4710
                     /locus_tag="ACHGNW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6926128.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4734..6206
                     /locus_tag="ACHGNW_001842"
     CDS             4734..6206
                     /locus_tag="ACHGNW_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6926129.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6257..6868
                     /locus_tag="ACHGNW_001843"
     CDS             6257..6868
                     /locus_tag="ACHGNW_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6926130.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6898..8811
                     /locus_tag="ACHGNW_001844"
     CDS             6898..8811
                     /locus_tag="ACHGNW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN6926131.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8939..11920
                     /locus_tag="ACHGNW_001845"
     CDS             8939..11920
                     /locus_tag="ACHGNW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN6926132.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11917..13578
                     /locus_tag="ACHGNW_001846"
     CDS             11917..13578
                     /locus_tag="ACHGNW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN6926133.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13618..14550
                     /locus_tag="ACHGNW_001847"
     CDS             13618..14550
                     /locus_tag="ACHGNW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN6926134.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4828 a         2343 c         2984 g         4704 t
ORIGIN      
        1 tttattcaaa tttgtcttca ccaacacgat ttgacgaaga gcctatttaa ataactacat
       61 ccatttattc tatcttctaa aatagccttt ttcatttcag ttaaatagtt cagcgccatt
      121 tttgcactcg acttgcatag tagttttttc catattcctc aacctcagcg ggggttgaag
      181 acataaggaa aatcctatgc gttatgctca actggatctg attaaatatt ctaatgtatt
      241 tttctctacc atcattaaat ggttaatagc caaaaatcac cacttttcaa tggctccttg
      301 ttgtataagt ataactagaa gatgaacacg tctgagaaat tatagaagct ttttacggta
      361 attactgggg ctgtgtccag tagttttttt gaagagaaca gaaaaatagc tcacgtgatc
      421 tattcctact ttttgactaa tttcatcaat agataagaat gtgtctttca gtagagcact
      481 tgctcttttg attcgaaagt ctagcaagta atcatgaaag gaataacctg tttctttttt
      541 gaaggcccgt cctaaataag cgggatggta atggaattgt gtagctacat ctttgagcgt
      601 aagcttttca ctataattgt tttgaacgta atcaatcact tgttgaattg actcattaaa
      661 agaatcatca gttagagagt tttgattagc tgctgattta cgcatttttt cttcttgtac
      721 taacttaact aaataaggaa aatcaatggg tttcgttatg taacttttaa tagataaatc
      781 aatccccttt tttgcatact gaaaattttg gtgggcacta atgacaatac tagtaaagta
      841 gcgattggat gtttgttgca aagattcgat taattcgagt ccagaaattt tccccatcgt
      901 aatatctgtg ataagtaaat caaagcgctg gttttgcagg tttttaaatg cttcgtctcc
      961 gtctgtgtag cagtgatcga taatgatgtc ggggactttc ttcaaaagat aatgccttat
     1021 gccttctaca attgtacaat catcgtcaac aattgcaatt ctcaacatgc atattccccc
     1081 ctttttgtat tttgatagta atcgttgttc cgacgtgtag ttggctttct ataacaaaac
     1141 gtgtatctcc aggatagaga tgctttatac gtcgaaaaga atttgagatg ccgatattct
     1201 cactataagg aatatcatca ttaagttttt gttgaatatg ctccagcttg tcttggtcca
     1261 ttcctttccc attttcaacg atttttaaca cataatggtt ggttgtttca gttgcataga
     1321 cagtcaaacg attttccttt tttgatgaat cgaagccgtg tttaatataa ttttcaacaa
     1381 atggctgtaa agaaagtttt ggtaacatta attgatcaat atgatcaatc agctgatagt
     1441 caaagttatg tcgataacga cgttgatgta gttgcaagga gaggtcgata ttaattaatt
     1501 cctctttcaa atcaattatg tcgggctgat atgtattatt gcgatacaac ttgctcaata
     1561 aatatatact gtcgctgatt tcttcgtcat cattcaatat ggcagtcatt cgaataaatt
     1621 ccaaattatt aaatagaaaa tgaggctcga tttgatattg aagtgctgca tactttgctt
     1681 cttgcttcaa ccaattattt tcataaagtg ttttttgata tgtagcatta tctaaataaa
     1741 actgattgat atgcatagcg atttctttca aatcaccgac tttgttttcg atattgattg
     1801 acttgtagtc tcctgtagat gaatgattta aggaagtaat gatttcctga tactgctgga
     1861 tatagttacg aaaacttcta tttaataatt gatgaatagc aagaataaac aaactcatta
     1921 ataagaaaat catgattgcc agacctaata aggtttgtga cgaataattt ggattttcat
     1981 atacttgtaa tgtgtggtta ccgatagcta tctgttgcgt ttttttccat ttagaaggcg
     2041 agatgtcttc agataaattg acggaatcaa taccattaga caaagaataa ttgtaatgtg
     2101 ttggaaagag actctttttt agcgcgttga tcgaaacgtc tagaatcaat gtagcatcgg
     2161 gcatgtcctc actaatgaaa cttaatgaac gaataaaaga aattttttcg ttttttgtag
     2221 gtgcgtaggc acttaagtat ttttcgtaat ctaatgagat agatatattt ttaacttcat
     2281 catcttgcac aagttttttt aagttatcta ctaaataggt aggggagcga tcctttgaca
     2341 aaatatcttc catgtattca gcaggtgatg agaggtagta gtcattaagg ttttcttgga
     2401 aatcataatt ttgatagatc tctgtcacga taccggagag aatacgatta tatgtttcca
     2461 tatagtgggt gatttgctga atgttttgca ggttttgatt tttttctttt tcaatcacat
     2521 tgatatagga aaagaacaat gtgaaacagg caagtaaagt aataaaaaaa atcaatatgt
     2581 atgtaaaacg cataataact tttgataaat cgtttttcat tcaaagtcct cattttgata
     2641 atctctaatt ttatgaatca ttatagcaca tattacaagt gatgtaaacg ctttgcaaac
     2701 agggctactt atgattgtga atacagtttc cgtttaaaaa ctattctatt gtagaggttt
     2761 aataaactaa aaataatgta taaaaaatta atgtaagcga ttaaccaagt gggtataatc
     2821 aaggaaacgc tgagagagga gagacaaaat gaagctaaag aaagggttat taggaaatct
     2881 aaaaaaaaac cgagcattgt ttattatgct tttaccaggt gcgttatggt tatttatatt
     2941 ttgctatata ccaatgtttg gagttgtaat cgcttttaaa gatttcagaa tttcatcaag
     3001 gggattcttt acaagtttgt tcaacagtcc ttgggttggc tgggaaaatt tcaaatatct
     3061 ctttgcttcg caagatgctt ggatcattac tcgcaatacg gttgtttata acgttgtagg
     3121 tattttgcta agtacttttt gtacagtgtc attagcaatc attctgagcc aaatggcgaa
     3181 caaacggtta ttgaaaaaga tgcagacatt tatgattttt cctaattttt tatcatgggt
     3241 agtcatcagt tactttgttt acgcattttt gagtacggat aaaggattaa ttaatatttt
     3301 actaagcaat tttggtgtgg aacccataaa ttggtatagt gaaccgaagt actggccttt
     3361 tattattgtt tttacgagtt tatggaaagg cgtaggctat ggatccatca tttattacgc
     3421 gtcaattatg gggatcgata ctagttatta tgaagctgct ttagttgatg gtgccagtaa
     3481 atggcaacag ataaggaatg tagtcttgcc acatttaaga tcgattattg tcattatgtt
     3541 gattttgtct gttggtagta tttttcgagc tgattttgga ttgttttatc aattgccaag
     3601 aaattcgggg gctctttatg atgtgacgaa tgtattagat acttatgtct accgtggatt
     3661 aatgaatagc ggagatatcg gaatgtctac ggcagcggga ttatatcagt catcagttgg
     3721 ctttgtcttg attttgatta ctaataagat agtcaacaag ttagatccta gttcagcact
     3781 attttaggag gaatcaaaat gatatcaaaa ttaaaacggc agatgtactt ttcgctgcca
     3841 gtcgaacggt ttttcacaat attactaatc ttatttactc ttagctgtat ttttcccttt
     3901 attttcgtaa tcgtcatttc attaacagat gaaaaagcat tacaactcaa cggttacgaa
     3961 ttattgccgg ctcaatggag cactgatgca tatcaatact taatacaaga tggcggacag
     4021 ttacttcggt cattaggcgt tacaattatg attacagtga ttggaacgtt gattactgtt
     4081 tttatgacgg gaacctatgc ttatgtttta tcaagagcct ctttccctta ccggaagttc
     4141 tttacttttt atcttttttt taccatgtta tttgcgggag ggatggttcc ttcttactta
     4201 gtgatgacga agatgcttgg cttaaaaaat acaatttggg ctttgattct acctcttgct
     4261 tttagcccat acaatgtcat tattttaagg acattcttta agaagtcgat tccagaatcg
     4321 attatcgaat ccgctaaaat ggatggctgt agtgagtttc gagttttttt ccaaatcgtt
     4381 ttgcctttag cgatcccagg ggttgcaaca ataggtttat tttcaagctt aggttattgg
     4441 aatgactggt tcaacgcatt gctgtatatt gacaccaaca aattgattcc attgcaatat
     4501 ttgttaatga agattcaaaa ttcaatggaa ttcttagcaa ataataatga cattacatta
     4561 gcgcagcaac aagcaattca aaatagcctt cctcaagaat caacacgaat ggcaatggtc
     4621 gtagtagcta ctttaccgat tgcaattgtt tatccgttct tccaaaaata ctttgttcaa
     4681 ggattgacga tcggtggagt gaaagaataa aaaagaatag gagatgttaa aagatgaaaa
     4741 agaacatgca gaaatatttg attggtggtg cttctgtact aacccttggc ctattaggtg
     4801 cttgtggtag cagtaatggc gaagcaaata atgaaacagg aaccacaaaa gttacttggt
     4861 atcaggttgg agaagaaccc aaagattttg atgaggtcat gacaaaagtc aacgaaaaat
     4921 taaaggaaga aatcaatgta gagttggata tgcggctgat tccggacggg gattatcagc
     4981 aaaagcttgg tgtgatgatc aattcaggag aagaatatga tatttgtttc gtcaatggca
     5041 cagattatgt gaattatggg aacaaaggtg ctttcattag tctcaacgat catttggata
     5101 aagagttgaa agattatgca gctgaattga atgaaggatt tattgaaggt ggggcaatta
     5161 atggcgaaac ttatggattg ccagtcaata acgatattgc cacaacggaa tattggacct
     5221 ttaataaaga tttggtagat caataccaat tggatgtaga gaatgttgaa ggtgtagaaa
     5281 cattggctcc agtattagaa aagtttgcag aaaaaaatac tgatacgaat attacgccaa
     5341 tcggtatgac ccgtaccagt gtctgtattc cttatgacaa tattatcggt ggtggtgtac
     5401 cgctagcggt tgcatttgaa ggcgatacac aaacagttgt taatacttat gagactccag
     5461 aaatgatgac ttatttgaag gctatgcatg agttttataa aaaaggatat attttgaaag
     5521 atgcagccac tgttacgaca gatccatttc gaattgatag taaaaactgg tttttaggtc
     5581 gtaactcaag tggttatcaa ccattagcac aaaaaaaatt agaagctcta gcgggacatg
     5641 gattaatttt aaaaccagct ggaaaagctt ttaaaacgac tggatttgca agagtggcaa
     5701 tgcatgcggt atctgcttca agtaaacatc cggaagcagc cttgaaattg ttgaattatg
     5761 tgaataccaa tcaagaagta gcggatttaa tggtttatgg aattgaagga aaacactatg
     5821 atcgcaatga agatggttcg attactttaa caaactctga tgattattat caaggcgctt
     5881 gggcattggt aaatacttat ctagtttcaa aaacttcacc gattacagaa gaaagtaaaa
     5941 aactagaaga agaaagtaaa aagtttcaaa atgaagcacc gatgtctcct attttaggtt
     6001 tctcttttga cgcttctgat tttcgaacag aaattgcaag tgtacaaaat atcgtgtcac
     6061 aatatgaagt acttttaagc tcgggaacag ttgaccctga acaatatgta ccagagttgt
     6121 tagaaaaact agaaaaagct ggattggata aaattcaggc atctgctcaa gaacaatacg
     6181 atgaatggcg aaaatctaac aaataatttt ttactaaatg aaaagaatag actaagcagt
     6241 tagtgggggg attcaaatga acgaaaacaa atggaccaaa ggatttaatt gggtttttca
     6301 attgttgtta ctaaacttta cttattttat actaatttgt ttaggatttg gaattttagg
     6361 ttttttccct gctacgatag ctttgattgc tggcgttgtg aatcgaaaaa aatatggctt
     6421 gcaagagtct gtgaagttca tgagtaaaat ctataagaaa aattttgtta gatataatct
     6481 atattgctgg tcagcaactt tgttgttggc cggcaatatt actacgatct ggctttgttg
     6541 gcagcaaagc gcaccattgt tttatttatt aagtacatta ttatcggtaa ttacactgtt
     6601 cctagtcttt gcaatgttga attgggtcgt gatcaaaagt cttgggttgt ggatgatggg
     6661 agaccgactg attgactatt tgcttttcac agcaacgatg ttgccaacgg tagtcatgca
     6721 aggcttggtg cttggaattt atttccgaat attgatgatt ttgcctgtga tttttctgtt
     6781 tggcggaaca gctgtaacgg taatattgat tagtaaatta actgaaagag cgttagcaaa
     6841 gttgccttgt cagatacaat cgcactaatt ttttcacaaa aaggagtatt tctttctatg
     6901 aagttaactg ataaacaagt agctaatata gcctttatcc agcaacttga gattccattg
     6961 acacaaaaag atgaagaaca aatagacttg atttttactg acacaaaggc taatgtgcga
     7021 caaatcaaga ttactaaaaa tgagcagcaa cgaatttgct tgtcttattc acatgaagac
     7081 ttgctgtatc gaggattgtt tatctgcctt ccacgttttt tagctcaagg aggagagatg
     7141 ccatacattg aagaaacctc gatcattgag accagttatt cattagatgc ttctcgtaat
     7201 ggcgtgatga ataaagagac tctaaaaaaa ttcatttctt acttagcgtt gatggggtac
     7261 aacacattgt atatttacac agaagatacc tatgaagtag aagaccttcc atattttggt
     7321 taccttcgcg gagcgtatac aaaagaagaa gtgaatgaaa ttgttgaatt tgcttctggt
     7381 tttggcatag aagtagttcc atcgatccag acccttgctc acttgaccca atttttgaag
     7441 tggtatgctc aacaagaact gatggatgat caaaatactc tactgatcgg tgaagaaaag
     7501 gtttatgcgg caatcgaaaa gatgattatt gcctgcaaag aaatgtatca gacagagcgt
     7561 attcatttgg gaatggatga agcctatcaa gcaggtctgg gacgatattt aagcctgtat
     7621 gggtattgtg atcggacaac tttaatgatt caacatatgg accgtgtgtt atcaattgtt
     7681 gataaatata agatgaaacc aatgatttgg agtgatttca tttataagct attagatgaa
     7741 accggtaaag ataggtatta caatcctgat tttaaactca aagcattagc agctaaagga
     7801 ttaccagcag gattaaccta tgttcattgg gactatggtt cagatgaagt ttcctcctat
     7861 gagaaagtaa ttgataacca tttgaaattt tgtgacattg atcattacgt tttagctagt
     7921 ggtgcccata tctgggggag actagccccg aatcatggga aaacagaagc aattgtttct
     7981 gcagctgtaa aagcttctaa aaataaaggt gtacgttctg tgatgttaac tacttgggga
     8041 gatgaagggc aggaaacaga gcattggcat tccttaattg gggcattaaa attagcagat
     8101 tatgtctatt ctgaagaaga gtggaatgat tttgaggaaa tctacgatcg tttattagga
     8161 acaggtagtt atcaattaat gtccgaatta cgatatttcg atgaagtgga taccattaca
     8221 aataacaact tacaaatggc ttccgtgagt aaactgctac tttggcaaga tccgttagtt
     8281 ggtatatatg actttcatgt agccgctcat aacaaaattg ccactagaac aattggtact
     8341 tattatcatg agttagctca gaaattagat atgaataaaa attggaatcc aaaacaacca
     8401 attttaacaa ctattcgtca acgatatata cttttagctg aaattttagc acaaaaggca
     8461 gaactaggca tctatttaca acactgctgt ctggtagaag accttgcagg attaatggaa
     8521 ttagcgaatc ggtgtgaagc actagccaat cgagtaaatg aattggcatc tattcatgaa
     8581 ataatttggt atcaaacata caaaacctat ggttgggaag tattagaaca acgatatgct
     8641 gcattagctc atcgtctgaa aaccgttgcg cgtcgtatta aaaatcatct tcaggggcaa
     8701 gatgccctag aggagctttt ggaagaacga ttaccgtttt gtcaatatca agtgccgctt
     8761 gaaatttctg gctttaatta tcggcaaagc tcggtgtcag gctacaatta acgaaggaat
     8821 cttcccgttg aacggaccac ttgctcatct aatgggaaag tggttcgttt tctattcata
     8881 agtagcttac cactaactct tttattaaga agagaaaaaa agtaggaagt gagaaaatat
     8941 gttatcggac tattatcaaa atctgtcagt tataagtgtc aatacagaac cattacgtaa
     9001 ttattatatt ccttatgcaa gtaaagaaga agcaaaacaa gagttagaaa gggaaaaaac
     9061 gagccagttt atcgatctca atggtacatg ggattttcag tattatcctt ctgtccaaca
     9121 gttaccggaa aatttttttc aagaggcctt tactgaaagc tatgatagaa tttctgttcc
     9181 tggagtttgg caaacagagg gatttgactt acctcagtat atcaatgtat cttatccaat
     9241 tccttttgat ccgccttatg tacctaatga taccccttgt ggtgtatatc ggcgtcagtt
     9301 tcaaattgag caaacagatg cacgctttta cttaaatcta gaaggagcag atagtaattg
     9361 ttttgtttgg ataaatagaa actttgtagg ctatgaccaa gtgagtcata gtacatctga
     9421 atttgatgtg accaagtatt tacaggttgg ggttaatgaa attcaaattc tagtgatgaa
     9481 gtggtgtgat ggtacctact ttgaagatca ggataagtgg cgaatgagcg gtctatttcg
     9541 tccagtgtat cttcttcaac gaccaactat gcatctgaca agttatcaac tacggacgtt
     9601 gactgatcct aaaaaaggaa gcggaacatt aatggtttct tttcaacgat ttactcctat
     9661 tgaagtatcg ttacctattt cttacgaatt attccatcct gatggacgtc tcatagcgaa
     9721 aggggagtgt tactcggaac aacaaataaa tttggatgtt gaagattgcc ttttttggac
     9781 agcagaaacg cctaaattat atacattact tttaacatgt aatggtgaat attttaaaga
     9841 aaaagttggc tttcgagcac tgacgatcaa gaatcgtaag ttattgctga acggaacacc
     9901 tattaaatta tatggagtca actatcatga cgtgaatcag caaggcacgc cagtgatgac
     9961 ccgagagcag ttacgtgcag atttgttatt aatgaaaaaa ttaaatgtga atactgtacg
    10021 aacggcacat taccctaaat caccagattt ttatgaattg gcggatgaga ttggtctgta
    10081 tgtaatcaat gaagcagatt tggagtgtca tggtgtggtt aatctatatg atactgaagc
    10141 gaattatcaa ctaattgcta atgatccgac attcgaaaag ccgattatgg accgagtgat
    10201 ccgttctatt cttccttatc aaaatcgaag ctctatcatt atgtggtcta tggggaatga
    10261 atctggttat ggagtaaatt ttgaaaaagc tcaagcttta gcacggaaat tggatgattc
    10321 acggttgatt cataatgaac gctcgattga accagttatt gaccaagtga atgattttag
    10381 taacttggat gttattagtc aaatgtatcc cccactggaa gatgttcatc gtcattgtca
    10441 aagaagtgat actgaaaaac ctttttttat gtgtgaatat agccacgcta tgggcaacgg
    10501 tcctggagat cttaaaacgt acttcgattt gattcaacag tatgattcct ttcttggcgg
    10561 ttgtgtctgg gagtggactg atcatgcaat caatagagga actttagaaa cgccatctta
    10621 tttatacggt ggtgattttg gtgaaaaata tcacgacggc aatttttgcg tagatggttt
    10681 attgacccca gatcggcaat taacagtagg tgcattggag taccgtaata ttcattcgcc
    10741 tattcaactg attgctgtag atgaggagct gcaagaagta accttgagca atataactgc
    10801 ttttacagcc ggttcagcat ttaacctttc tcttgaatgg tatatagatg gaaaaaaaac
    10861 agtttatgaa gtaatgaatc ctttcatact tgagcctggc aaaagttgtt cattgcccat
    10921 cacagtacct acaacgattc atggtgctca tacggttgct gtttcgctat ctgtttataa
    10981 aaatgatgag ctttatggac agcaacagct ctttcttaaa aaagaaaaaa tgagattgcc
    11041 aagaactgct catcataatg atgcaccaca aatttcagta aaaggcacag agctgataat
    11101 caaaggagaa tcctttattt atatatatca attagcaaaa gctgcgttca cttctgtaaa
    11161 gatcaatcaa aaggaacttt tggaacgtcc tactcaattt tcaatttggc gtgcaccaac
    11221 ggataatgat cgtaacttag taaacaaatg gaaagcagct ggatatgctg aaatgtcttt
    11281 agaaatcaga aaaaaagact ggaaattcgt agatgatcat ctcctgatcg aaacgtctta
    11341 tcgaggagtt attccaggat tacagccaat ttttacaatg cacatacagt tgaaaataaa
    11401 tggtgcaggc gtaatcaacc aaaaagtatc agtggagaaa aatccgattt ttccagattt
    11461 ccctcgcttt gggatggact ggccgttgaa tgatactttt tcgaaggttt cttattgtgg
    11521 ctatgggccg tatgaaagtt atcctgataa gcactatgct gcgatgtttg gtatttatga
    11581 gatggagatt gaggaatttg atcagccata tatcaaacca caagaatatg gtaaccgctg
    11641 gcaatgtgat tgggtatcaa tcaaaaatca tcaaggatct accatgacgg tttatggaaa
    11701 ctctaatttt tctgctctgc cttatagtca agctcaatta accgaaaagt cacaccatta
    11761 tgaattaata aaagaaaata gaacttggtt caatttaagt tataaacaaa atggtatcgg
    11821 ctcacacagt tgcggtcctt atttagaaga tcgtttccgt tttatggaag atacttttga
    11881 ctggaatata gtgattactt tcaaaggaga aaatagatga aagaaacaaa gatcaatcaa
    11941 gtcaaaaaaa aattatctcg ttatcaagaa gttgtttatc gaaaccattg cttaaaatgg
    12001 tcaaaaaata agctgaatgt tcgttttaat ggattatttt atcgtttcga acagttagaa
    12061 gaagtctgga aaccggtaac gaatttccaa tcatgtcctg tacagctcat aaatgatcca
    12121 aaagaaacat acgatttatg tataacacac ctttataacc agcatttgaa aaatgacgga
    12181 ttttatttaa aggttactaa tgacaatcaa cttacgattg aatcagcaaa ccttcgaggg
    12241 tttcaatatg ctatggaagc cttattagag agtttttttt cggtaggaga tcaattatta
    12301 ttaccggaat tgattctgaa acatgaacca tcagtgaaaa ttcgaggaat cattgaaggg
    12361 ttttatggta tcccctggac acatgaaatg cgcctctctc ttttttcata tatgaaagat
    12421 aaccaattga atacttttat gtatgcccct aaagatgatg aattattacg taaaaaatgg
    12481 cgtgaactct acgatgcgca agaattagac aaatttaagg agcttttatc tgctgctgag
    12541 gctagcaata tcgatttttg gtacttaatt agtcctggaa atgatattga tattacgtgt
    12601 gaagaagaca ttcaagttct tctgaaaaag ttagaacaac tgattgagct tggcgtattt
    12661 caatttggtc tattaatgga tgatattgac tatcaattaa aaggtgctgc taaacgccgt
    12721 tttcgagagc cagcattcgc tcatgcctat ttagtgaatc gagtagagga atatcttagc
    12781 actcggttgt ccaatcatga attagtcatt tgtcctacag aatatgataa tcgtcatggt
    12841 tctcgctatt tagcaacgtt aagtcaagaa ataccagaac aattaccgtt cttttggact
    12901 ggcccaagca cgttagcggc gtcaatttct acagaagagc tgcaggaaat ggcttctgtc
    12961 tatcaacggc cgatgctgat ttgggataat attccggtaa atgattattt agaggataaa
    13021 gaactattgt tcatgagtcc atatgagaat cgaacaccga atctttctaa ggagagatac
    13081 caggttacag gagtagtttc gaatccaatg gctcaattag aagcttcaaa gtttacgatc
    13141 aatagtatgg ctaattactt atggaactgt gagcgttttg atcctttgga aacttggacg
    13201 tcagtcgttg aaaaagtagt tggaagtaaa ctacaaccag cttatcttac cttaaccaat
    13261 gcttttccaa atcattacac aagcagtctg ctttcagatg aagaattact gctagccaaa
    13321 gacccagaat gggtcacaaa agaaatggct gctttagtcc aagcagtaaa atggatccag
    13381 caagaacaac atacatctag attgagaaca gaattggagc cttggttaca agcaatcaca
    13441 gaaagttgga ctttctggca agaatggcaa ttaaaaaaag atccaaaaga agcagaagaa
    13501 tggttggcta agaaaaaaac acaccgcata ggacgtgatt tagtaactgc ccatttagag
    13561 caaattatca aaagttagat atcttaaaaa ataaaaagca agaaaaagga gaacaaaatg
    13621 gtcaagagat taattagtgc aaactacagt gatgtcaaga aaatgacgtc aaaagagttg
    13681 aagcaatcca tcaaagcgag tgaaggacgg gtgattgttt gtgaaacggt tgctaatact
    13741 cttccacaat ctggagaaat tacgaatgca gaagttgcgt cagcatttgg tgcagatttg
    13801 attcttttaa atggtttcga ttgttatcgt cctgcagttt ttggactacc aaatgtgacg
    13861 attgagaagg tttttgatct acagcaaacg attgaaaacc cgattcctaa attaaaagaa
    13921 ctagtcggtc gtcctgtggg aattaattta gaacccattg cccctaaaac aaatatgatg
    13981 agtgagaaag tagaaatcag ccaaggaaga gcaagctctg ttgaaacgat acaaaaagca
    14041 gaagaactag gagtcgattt tatttgcttt accggtaatc caggcactgg agtaaccaat
    14101 ttagaaatta ctaaatccgt aaaaatggca aaaaagtatt tttcgggatt gattattgct
    14161 gggaaaatgc atagcgccgg ctcagaagag ccagttgtga ataaagaggc gattatttct
    14221 tatgcggaag caggcgcaga tattttacta gttccttcag tagggtctat cccaggcttt
    14281 ttagagcaag acctaattga agcggttcgg ttggcaaaaa aatatgattt gttaacgatg
    14341 tctgcaatcg gtactagtca ggaaagtgcc cgaccagaaa cattgcggca attagccatt
    14401 atgaataaaa gttgtggtgt tgatttacaa catattggag atgctggata cgggggattg
    14461 gcaccagcag aaaatatttt cgaaacctcg gttgcaatta gaggaatgcg acataccatc
    14521 aactgcgtta gccgctcgat caatcgctaa caaaattaaa taaaaaatgg ttaaattgat
    14581 tatttaagtg ggaacatact tgagatatca gctaacttct gtcactttaa aaaagggact
    14641 gtgacagaag tgaaaataga caaaaaatat ccgaacaaga gactaggata gcagaaaaac
    14701 tatcgttttg caacgaggac caactggacc gtaggagcaa ttctcttatt gttcggatat
    14761 ttcgcttgtt caaggactta tgtctgtagt gccctttttt ggtaggaaaa atggtaggga
    14821 accatatcat ttttaatgaa aatcaagcat ttcaaaagt
//
LOCUS       DAZBTH010000063        14344 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000063 DAZBTH010000000
VERSION     DAZBTH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.064"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACHGNW_001848"
     CDS             <1..83
                     /locus_tag="ACHGNW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFN6926135.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            110..445
                     /locus_tag="ACHGNW_001849"
     CDS             110..445
                     /locus_tag="ACHGNW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926136.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            622..1572
                     /locus_tag="ACHGNW_001850"
     CDS             622..1572
                     /locus_tag="ACHGNW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6926137.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2500
                     /locus_tag="ACHGNW_001851"
                     /pseudo
     CDS             2090..2500
                     /locus_tag="ACHGNW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2943..3755
                     /locus_tag="ACHGNW_001852"
                     /pseudo
     CDS             2943..3755
                     /locus_tag="ACHGNW_001852"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3750..3914
                     /locus_tag="ACHGNW_001853"
     CDS             3750..3914
                     /locus_tag="ACHGNW_001853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926138.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3854..3976
                     /locus_tag="ACHGNW_001854"
                     /pseudo
     CDS             3854..3976
                     /locus_tag="ACHGNW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4538..5044)
                     /locus_tag="ACHGNW_001855"
     CDS             complement(4538..5044)
                     /locus_tag="ACHGNW_001855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6926139.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5049..5816)
                     /locus_tag="ACHGNW_001856"
     CDS             complement(5049..5816)
                     /locus_tag="ACHGNW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6926140.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="ACHGNW_001857"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="ACHGNW_001857"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFN6926141.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="ACHGNW_001858"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="ACHGNW_001858"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFN6926142.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="ACHGNW_001859"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="ACHGNW_001859"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFN6926143.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="ACHGNW_001860"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="ACHGNW_001860"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN6926144.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="ACHGNW_001861"
     CDS             9217..9534
                     /locus_tag="ACHGNW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6926145.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="ACHGNW_001862"
     CDS             9534..11240
                     /locus_tag="ACHGNW_001862"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN6926146.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="ACHGNW_001863"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="ACHGNW_001863"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN6926147.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="ACHGNW_001864"
     CDS             12811..13083
                     /locus_tag="ACHGNW_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFN6926148.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="ACHGNW_001865"
     CDS             13104..14003
                     /locus_tag="ACHGNW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN6926149.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DAZBTH010000064        14337 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000064 DAZBTH010000000
VERSION     DAZBTH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.065"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..3860)
                     /gene="rpoC"
                     /locus_tag="ACHGNW_001866"
     CDS             complement(207..3860)
                     /gene="rpoC"
                     /locus_tag="ACHGNW_001866"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN6926150.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3939..7556)
                     /gene="rpoB"
                     /locus_tag="ACHGNW_001867"
     CDS             complement(3939..7556)
                     /gene="rpoB"
                     /locus_tag="ACHGNW_001867"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6926151.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8284..9252
                     /locus_tag="ACHGNW_001868"
     CDS             8284..9252
                     /locus_tag="ACHGNW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6926152.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9270..10175
                     /locus_tag="ACHGNW_001869"
     CDS             9270..10175
                     /locus_tag="ACHGNW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926153.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10172..10978
                     /locus_tag="ACHGNW_001870"
     CDS             10172..10978
                     /locus_tag="ACHGNW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926154.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11135..12103
                     /locus_tag="ACHGNW_001871"
     CDS             11135..12103
                     /locus_tag="ACHGNW_001871"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN6926155.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12420..12944)
                     /locus_tag="ACHGNW_001872"
     CDS             complement(12420..12944)
                     /locus_tag="ACHGNW_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN6926156.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13231..>14337
                     /gene="scm"
                     /locus_tag="ACHGNW_001873"
     CDS             13231..>14337
                     /gene="scm"
                     /locus_tag="ACHGNW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6926157.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSD"
BASE COUNT         4065 a         3089 c         2817 g         4366 t
ORIGIN      
        1 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
       61 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
      121 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
      181 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
      241 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
      301 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
      361 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
      421 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
      481 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
      541 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
      601 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
      661 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
      721 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
      781 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
      841 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
      901 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
      961 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
     1021 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
     1081 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
     1141 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
     1201 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
     1261 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
     1321 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
     1381 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
     1441 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
     1501 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
     1561 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
     1621 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
     1681 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
     1741 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
     1801 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
     1861 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
     1921 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
     1981 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
     2041 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
     2101 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
     2161 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
     2221 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
     2281 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
     2341 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
     2401 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
     2461 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
     2521 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
     2581 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
     2641 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
     2701 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
     2761 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
     2821 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
     2881 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
     2941 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
     3001 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
     3061 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
     3121 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
     3181 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
     3241 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
     3301 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
     3361 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
     3421 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
     3481 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
     3541 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
     3601 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
     3661 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
     3721 aacaaccctt cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
     3781 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
     3841 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
     3901 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
     3961 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
     4021 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
     4081 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
     4141 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
     4201 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
     4261 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
     4321 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
     4381 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
     4441 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
     4501 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
     4561 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
     4621 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
     4681 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
     4741 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
     4801 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
     4861 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
     4921 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
     4981 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
     5041 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
     5101 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
     5161 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
     5221 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
     5281 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
     5341 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
     5401 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
     5461 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
     5521 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
     5581 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
     5641 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
     5701 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
     5761 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
     5821 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
     5881 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
     5941 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
     6001 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
     6061 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
     6121 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
     6181 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
     6241 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
     6301 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
     6361 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
     6421 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
     6481 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
     6541 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
     6601 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
     6661 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
     6721 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
     6781 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
     6841 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
     6901 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
     6961 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
     7021 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
     7081 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
     7141 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
     7201 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
     7261 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
     7321 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
     7381 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
     7441 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
     7501 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
     7561 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
     7621 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
     7681 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
     7741 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
     7801 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
     7861 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
     7921 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
     7981 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
     8041 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
     8101 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
     8161 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
     8221 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
     8281 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
     8341 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
     8401 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
     8461 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
     8521 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
     8581 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
     8641 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
     8701 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
     8761 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
     8821 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
     8881 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
     8941 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
     9001 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
     9061 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
     9121 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
     9181 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
     9241 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
     9301 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
     9361 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
     9421 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
     9481 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
     9541 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
     9601 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
     9661 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
     9721 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
     9781 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
     9841 ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt
     9901 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
     9961 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    10021 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    10081 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    10141 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    10201 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    10261 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    10321 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    10381 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    10441 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    10501 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    10561 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    10621 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    10681 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    10741 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    10801 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    10861 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    10921 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    10981 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    11041 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    11101 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    11161 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    11221 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    11281 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    11341 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    11401 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    11461 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    11521 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    11581 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    11641 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    11701 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    11761 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    11821 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    11881 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    11941 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    12001 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    12061 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    12121 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    12181 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    12241 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    12301 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    12361 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    12421 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    12481 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    12541 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    12601 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    12661 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    12721 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    12781 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    12841 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    12901 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    12961 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    13021 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    13081 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    13141 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    13201 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    13261 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    13321 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    13381 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    13441 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    13501 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    13561 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    13621 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    13681 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    13741 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    13801 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    13861 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    13921 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    13981 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    14041 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    14101 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    14161 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    14221 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    14281 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgat
//
LOCUS       DAZBTH010000065        14043 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000065 DAZBTH010000000
VERSION     DAZBTH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.066"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHGNW_001874"
     CDS             <1..106
                     /locus_tag="ACHGNW_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6926158.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            255..593
                     /locus_tag="ACHGNW_001875"
     CDS             255..593
                     /locus_tag="ACHGNW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926159.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(798..1373)
                     /locus_tag="ACHGNW_001876"
     CDS             complement(798..1373)
                     /locus_tag="ACHGNW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFN6926160.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2105..2686)
                     /locus_tag="ACHGNW_001877"
     CDS             complement(2105..2686)
                     /locus_tag="ACHGNW_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6926161.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2869..3495)
                     /locus_tag="ACHGNW_001878"
     CDS             complement(2869..3495)
                     /locus_tag="ACHGNW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6926162.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3517..4848)
                     /locus_tag="ACHGNW_001879"
     CDS             complement(3517..4848)
                     /locus_tag="ACHGNW_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN6926163.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5003..5404)
                     /locus_tag="ACHGNW_001880"
     CDS             complement(5003..5404)
                     /locus_tag="ACHGNW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6926164.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5490..5696)
                     /locus_tag="ACHGNW_001881"
     CDS             complement(5490..5696)
                     /locus_tag="ACHGNW_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926165.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5791..7293)
                     /locus_tag="ACHGNW_001882"
     CDS             complement(5791..7293)
                     /locus_tag="ACHGNW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN6926166.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7311..7766)
                     /gene="argR"
                     /locus_tag="ACHGNW_001883"
     CDS             complement(7311..7766)
                     /gene="argR"
                     /locus_tag="ACHGNW_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6926167.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7864..8481)
                     /locus_tag="ACHGNW_001884"
     CDS             complement(7864..8481)
                     /locus_tag="ACHGNW_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6926168.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8702..10075
                     /locus_tag="ACHGNW_001885"
     CDS             8702..10075
                     /locus_tag="ACHGNW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6926169.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10150..11262
                     /locus_tag="ACHGNW_001886"
     CDS             10150..11262
                     /locus_tag="ACHGNW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6926170.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11334..12971)
                     /locus_tag="ACHGNW_001887"
     CDS             complement(11334..12971)
                     /locus_tag="ACHGNW_001887"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN6926171.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13139..13747)
                     /locus_tag="ACHGNW_001888"
     CDS             complement(13139..13747)
                     /locus_tag="ACHGNW_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6926172.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3832 a         2929 c         2494 g         4788 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagaaacta
      121 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
      181 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
      241 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
      301 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
      361 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
      421 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
      481 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
      541 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
      601 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
      661 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
      721 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
      781 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
      841 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
      901 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
      961 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     1021 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcataaaa gccactcatt
     1081 gggaaaacac aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc
     1141 tctgcacggg cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg
     1201 atttccggag tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga
     1261 aaaacttctc cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga
     1321 taataattct gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc
     1381 cctcccaatc tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt
     1441 tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt
     1501 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt
     1561 gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg
     1621 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     1681 cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg
     1741 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta
     1801 tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta
     1861 aacggtgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
     1921 tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata
     1981 cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga
     2041 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta
     2101 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
     2161 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
     2221 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
     2281 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
     2341 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
     2401 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
     2461 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
     2521 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
     2581 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
     2641 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
     2701 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
     2761 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
     2821 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
     2881 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
     2941 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
     3001 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
     3061 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     3121 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     3181 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     3241 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     3301 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     3361 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     3421 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     3481 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     3541 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     3601 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     3661 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     3721 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     3781 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     3841 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     3901 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     3961 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     4021 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     4081 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     4141 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     4201 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     4261 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     4321 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     4381 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     4441 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     4501 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     4561 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     4621 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     4681 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     4741 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     4801 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     4861 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     4921 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     4981 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     5041 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     5101 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     5161 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     5221 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     5281 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     5341 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     5401 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     5461 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     5521 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     5581 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     5641 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     5701 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     5761 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     5821 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     5881 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     5941 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     6001 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     6061 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     6121 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     6181 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     6241 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     6301 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     6361 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     6421 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     6481 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     6541 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     6601 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     6661 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     6721 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     6781 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     6841 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     6901 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     6961 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     7021 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     7081 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     7141 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     7201 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     7261 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     7321 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     7381 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     7441 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     7501 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     7561 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     7621 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     7681 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     7741 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     7801 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     7861 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     7921 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     7981 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     8041 taagatttgg gcacaaagac cttcttccat ctttttgaat actagttgtt ctttcaagtc
     8101 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     8161 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     8221 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     8281 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     8341 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     8401 tggtttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     8461 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     8521 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     8581 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     8641 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     8701 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     8761 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     8821 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     8881 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     8941 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     9001 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     9061 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     9121 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     9181 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     9241 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     9301 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     9361 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     9421 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     9481 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     9541 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     9601 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     9661 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     9721 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     9781 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     9841 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     9901 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     9961 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
    10021 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
    10081 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
    10141 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gactctagcc
    10201 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
    10261 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
    10321 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
    10381 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
    10441 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
    10501 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
    10561 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
    10621 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
    10681 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
    10741 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
    10801 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
    10861 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
    10921 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
    10981 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
    11041 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
    11101 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
    11161 tatatcggct atctattagc caacttctta aatactggtc attggtctac ggaagattat
    11221 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
    11281 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
    11341 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
    11401 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
    11461 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
    11521 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
    11581 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
    11641 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
    11701 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
    11761 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
    11821 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
    11881 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
    11941 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
    12001 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
    12061 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    12121 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    12181 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    12241 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    12301 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    12361 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    12421 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    12481 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    12541 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    12601 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    12661 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    12721 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    12781 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    12841 gctgtcttcc tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    12901 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    12961 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    13021 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    13081 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    13141 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    13201 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    13261 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    13321 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    13381 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    13441 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    13501 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    13561 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    13621 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    13681 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    13741 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    13801 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    13861 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    13921 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    13981 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    14041 ttc
//
LOCUS       DAZBTH010000066        13810 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000066 DAZBTH010000000
VERSION     DAZBTH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.067"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHGNW_001889"
     CDS             complement(221..1423)
                     /locus_tag="ACHGNW_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926173.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHGNW_001890"
     CDS             complement(1426..1620)
                     /locus_tag="ACHGNW_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6926174.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHGNW_001891"
     CDS             complement(1667..2515)
                     /locus_tag="ACHGNW_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN6926175.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHGNW_001892"
     CDS             complement(2720..3070)
                     /locus_tag="ACHGNW_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926176.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHGNW_001893"
     CDS             3248..4111
                     /locus_tag="ACHGNW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6926177.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHGNW_001894"
     CDS             4735..5727
                     /locus_tag="ACHGNW_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926178.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHGNW_001895"
     CDS             5743..6627
                     /locus_tag="ACHGNW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6926179.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHGNW_001896"
     CDS             6676..8283
                     /locus_tag="ACHGNW_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6926180.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHGNW_001897"
     CDS             8284..9768
                     /locus_tag="ACHGNW_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN6926181.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHGNW_001898"
     CDS             9945..10937
                     /locus_tag="ACHGNW_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6926182.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHGNW_001899"
     CDS             10989..11858
                     /locus_tag="ACHGNW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6926183.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHGNW_001900"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHGNW_001900"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6926184.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZBTH010000067        13796 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000067 DAZBTH010000000
VERSION     DAZBTH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.068"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..882)
                     /locus_tag="ACHGNW_001901"
     CDS             complement(22..882)
                     /locus_tag="ACHGNW_001901"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN6926185.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            974..1402
                     /locus_tag="ACHGNW_001902"
     CDS             974..1402
                     /locus_tag="ACHGNW_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6926186.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1564..1740
                     /gene="rpsU"
                     /locus_tag="ACHGNW_001903"
     CDS             1564..1740
                     /gene="rpsU"
                     /locus_tag="ACHGNW_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN6926187.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1767..2213
                     /locus_tag="ACHGNW_001904"
     CDS             1767..2213
                     /locus_tag="ACHGNW_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN6926188.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2367..3746)
                     /gene="brnQ"
                     /locus_tag="ACHGNW_001905"
     CDS             complement(2367..3746)
                     /gene="brnQ"
                     /locus_tag="ACHGNW_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6926189.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4452..5423
                     /locus_tag="ACHGNW_001906"
     CDS             4452..5423
                     /locus_tag="ACHGNW_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN6926190.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5447..7639
                     /locus_tag="ACHGNW_001907"
     CDS             5447..7639
                     /locus_tag="ACHGNW_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN6926191.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7656..8132
                     /gene="ybeY"
                     /locus_tag="ACHGNW_001908"
     CDS             7656..8132
                     /gene="ybeY"
                     /locus_tag="ACHGNW_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN6926192.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8116..8511
                     /locus_tag="ACHGNW_001909"
     CDS             8116..8511
                     /locus_tag="ACHGNW_001909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6926193.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8526..9425
                     /gene="era"
                     /locus_tag="ACHGNW_001910"
     CDS             8526..9425
                     /gene="era"
                     /locus_tag="ACHGNW_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN6926194.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9568..10380
                     /gene="recO"
                     /locus_tag="ACHGNW_001911"
     CDS             9568..10380
                     /gene="recO"
                     /locus_tag="ACHGNW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN6926195.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10764..11681
                     /gene="glyQ"
                     /locus_tag="ACHGNW_001912"
     CDS             10764..11681
                     /gene="glyQ"
                     /locus_tag="ACHGNW_001912"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN6926196.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11683..13758
                     /gene="glyS"
                     /locus_tag="ACHGNW_001913"
     CDS             11683..13758
                     /gene="glyS"
                     /locus_tag="ACHGNW_001913"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN6926197.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4648 a         2478 c         2892 g         3778 t
ORIGIN      
        1 ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt gtttcagttc cataataact
       61 atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa tagataaaga
      121 tgccacattg atcactacac atcctagttt ttcataaaga tcattcgcaa aatcgagttc
      181 agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt aagagcgcat
      241 ccgttcttta cggatattat ttaaaatatc tgggttattc gttagtccca cgattttttt
      301 aggatcgatt tcccacagct gtttgggaat atgtgcttgg ggaatcaaag gtaaattagc
      361 aacttttaaa tttttatttg ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac
      421 gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat catcgtattt
      481 tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata aatggagggc
      541 accaggttgc cgaattggct tgataccagt taaacgttca atttctgata tcggtggcgt
      601 catcacatcg aaactaaata attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag
      661 ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat cttgcaatgc
      721 ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga actcaacggt
      781 aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag cagagtcgga
      841 gatgacaaaa aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc
      901 atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc attttgattt
      961 ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa cattgaaaaa
     1021 taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgtaa
     1081 ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca aaggggtaag
     1141 ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac tggaagaaac
     1201 cgatttaaat ggtgaaatga agtttcgttt tcattgctgt cagggggaaa tcggccatca
     1261 ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa
     1321 ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg agattttcgg
     1381 aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat ttaaaaaaaa
     1441 agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt
     1501 tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt
     1561 aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc
     1621 aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa
     1681 aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag
     1741 ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga
     1801 aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat
     1861 ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaactaacgg
     1921 tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac
     1981 gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg
     2041 aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact caaactggtg
     2101 cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa
     2161 aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct taattttatc
     2221 atttaaactg cggacagtga ttgtccgcag ttttttgtgt tcgtaaaaaa agaactaaaa
     2281 aacagctaga agaaagaata agattctttc ttctagctgt ttttttcctg tttaatatct
     2341 cttatgttaa attgtgtttg atttggttat tcttctggta gttcatttgc ttcttgttta
     2401 taccctgata ggcgataggc gtaataaaag agtaagctgc cagcgtaacc aagtaacgtt
     2461 gggaccatcc agccaagtcc taatgcagca aaaggaacgt gatgatgata aaatcctagt
     2521 aagcttgcta cgacagtttg ctgactcact aatgcaggcg aatttgccaa catatccaat
     2581 acggctggaa cggcagaaaa gaacatcgtt acttgataga caaatggact cttttggaag
     2641 aattttgctg ttaaggaaag caagattaac gcaagtgaca atggatacaa aagcatcaat
     2701 actggtaaag accattggat gatattatcc aatccggcat tcgcaacgac aaaagagaca
     2761 aaagtcgtta accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct
     2821 tgtgcaaaag aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccattacc
     2881 cctaagaagc tagcaccgaa gttacctaaa taaaaattag tgatctgaga aagtgcagtt
     2941 ccgccatttt ctgataaagg catcttattc aaactcatcg caccgagaag gaccagacca
     3001 aaataaatca tgacttctaa cgcaacgctc aggctaccag cttttgctgt taattctgtg
     3061 acttttttat ctttaaaacc aagatcacga actgcatgga caaaagtgac acttaaagca
     3121 agtaaagcta ctgcatcaac cgtgttgtat ccttctaaaa cgccatttgt caaagcgttc
     3181 gtctgataag aatctgcagc agattggttt agattcccca tgggttggaa taaagaaacg
     3241 acaaaaatga tagctaataa tgcaagaaac aaggagttta aatactttcc gacatatttg
     3301 accaaacgtg ttggatgtac agtaaggaaa taagcaagta aaaagaaaag agcagagaat
     3361 aaaaacatac ctaatgaatc atatttttca ggcaaaaagg gtttagcagc catttcaaaa
     3421 gcagttgctg ctgtccgcgg tgtaccaaag aatggtccga tcgttaaatg aacaaggatc
     3481 aagaacaagg cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt
     3541 tttgtcacta ctacagctaa gatagctaat aatgggaaaa gagcgcctga gatggcaaat
     3601 cctacaccag cgctgatcca gtttgatcca gccatttgac ctaagtggac aggaaagatc
     3661 agattacctg acccgaagaa catcccgaag ataagtgaac tgaccatcaa taattgcttc
     3721 catgaataag tttttgtttc ttccataaat ttttcctcca ataattatca ttttgagtaa
     3781 caaaataaac gttgcagaaa ctagaaatgc cactaatatc agattccatg aaaaccacca
     3841 ccttaaaata taaaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg
     3901 gtaccacctt ttttcgctta aaaaagcttc ttcattcacg gacgcttttc gataaagtta
     3961 tccgctagcc tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa
     4021 atgatgttcg tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa
     4081 tcgctgaata gcctactgtt ttcctcgacg ctttgctgta tatttatttg ttgacgttat
     4141 aatacacctg ttttttttga aatgcaagaa tatttcaaat attttgtttt ctttttttga
     4201 aacaagaaaa atacccagag aatacttgct aagttagacg gaatacttgt cctctatctt
     4261 atgcatagct tacatgatca gcttttttta ttcaaacgag acctttagag aatggaagca
     4321 gctatttttt aacgtttctt ggtacttatg gttaagcaac tcttttagaa ccttttctta
     4381 tttgctatat agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg
     4441 gatgtctatt tttgacggaa gagtctagtt ctttagagat tagattgacg gctgctgatg
     4501 atgtcagcat gcttttaggt gcacacgaca agcatataaa actgattgaa gaaacaacag
     4561 aaacaaccat ccatacacgt ggagaaatca ttcagatcat tggcgaacaa tcccagattt
     4621 ccttggctgc ctcagtcatt cggacattgc aggaattgat caaacgagga atccatgtaa
     4681 gtacaccaga tgtcgtgacg gcactgaaaa tggggcaaaa aggcacatta gattattttg
     4741 ttgaaatgta tgaggaagag atcgtcaaag accgtaatgg aaaaccaata cgggtaaaaa
     4801 acagcggcca aaagaaatat gtcgaatcgg tagccaaaca tgatatcgta tttggcgtag
     4861 gaccagccgg gacaggaaaa acttttcttg cagtagtatt agcaatcgct gccttgaaaa
     4921 aaggtgaagt gcaaaaaatc attttgacgc ggcctgctgt ggaagcaggg gaaaatctag
     4981 gctttcttcc cggggatcta aaagaaaaag tagatcctta tctgcgtcct gtttatgatg
     5041 ctttatacca aatttttgga ttagatcata cgaaccgatt gatggaacga ggcgtgatcg
     5101 aaattgctcc tttagcttac atgcgggggc ggacattgga tgatgcattc gttattttgg
     5161 atgaagcaca gaacacgaca gttgctcaga tgaaaatgtt cctgacacgt cttggttatc
     5221 aatcaaaaat gattgtcaat ggagatacga gtcagattga tctgcctcga gggacaacaa
     5281 gtggtcttgt tcatgcagaa cgaacattaa agcaaatcaa gaaaatcgat tttgttaatt
     5341 ttgaagcaag tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa
     5401 aggcagatct ccatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac
     5461 actgcagaaa ctacaagaaa aacttggaaa ggcagcattg cctttcctgc tgttcttgtt
     5521 ttttatcatt atctgcggcg ttacatttag tagtgtcaaa caaaaggcca atgatttcag
     5581 agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa acgatcgaga acacggaaga
     5641 aaccgaacaa aaaagaaaac tggcagcaga agcagtcact ccagaataca cttaccaaaa
     5701 agatcttgca gatgaccaga acaatcggat caagcaacta tttgaattga tcgataaaac
     5761 gaatgatgcc atcaacaaat cttatgacga aaagattgat aaggcaaagg aagatgaaaa
     5821 tatcccaaag cctactacag aagagcggat cgccgcgttg aagaaaaatt ttgaaaatgt
     5881 gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac aatttttatt cgacgatatt
     5941 tgaaatgact gaaactgaga tccatactgt gagggatgag agtctgaagc tcattgacga
     6001 acagatgagc aaacaagttc gtgaaagtga attggaagct tttaaacaag aagcagaaga
     6061 gcaaatccaa tatttgaatg tcacaccagc acaacaacaa atgattcgct acttagtgga
     6121 tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag acagaagaat tgaaacaatc
     6181 cgcacgagaa gctgtacaac cagtgatgat cttccaagga gaaatcattg ttcgtgaagg
     6241 taatcaaatc gatgcttcag ctatgaaaaa attggaatta ctaggactga ctaatcaaac
     6301 aacttcgatt tttccgctaa ttgccatggt tttagctgtt ttgcttcaaa tcggcgtact
     6361 gatttataat tcacttcaat tccaaaatgc aggagaaaga acaaaatacg tgcttttcta
     6421 tgtgacagct atgtcagtca gcatagtgct gatgaaattt ttccaattat tccaaacgga
     6481 acaagctact tatatcccgc tgttttatcc cgctgctttt gcaccacttg ttttaagctt
     6541 tttcttgaac cgaagagcag ggatcatggc tggtattttc caagcagtat ctgcactttt
     6601 cattttttat gattcgattg gcacgaactt tctaacgatt atcttgatgt cttatgtttt
     6661 ctctgggctc atggcaacga ttgtcagacg caaacggatc tcagaacaag gattatctgc
     6721 tgccatgtgg gtcattattt ttcctgtgat gatggatgtc attttgatcg tctatcaagg
     6781 aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc tgcgggttca ctggtgcact
     6841 cttctcattc ttgctgacga tcggtctaca cccatatatt gaattgatgg tcaatgatga
     6901 cagtgtaatt gtattaaatg aactcagcaa tccaaatcat cctttattga aacagttgct
     6961 ggaagaagcg ccaggtactt atcatcatag tatgatggta gctaatctaa gtgctaacgc
     7021 tgtagcagaa atcggcggac gttctttgct aactcgagta gcttgctatt atcatgatat
     7081 cggcaagatc aaacatgcta gctttttcgt tgagaacttg ccttcaggag cggaaaaccc
     7141 gcacaatttc ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg
     7201 agccaaaata ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcaaacagca
     7261 tcatggaacc acgctaatgc gttatttcta catcaaagcg aaagaacgca atccggaagt
     7321 aacagaagag caattccgtt atcctggacc aaaaccgcaa agtcgtgaag cagggatcgt
     7381 caatattgct gacagttgtg aggctgcagt acgggcaatg gatcatccga cgagcgaaaa
     7441 aatcgaaagt tttgtccata atcttatcga agagcgtatt tccgatggtc aattagacga
     7501 tagtggactg accttgaaag aaatccgaaa agtggaaaaa tcattgatca gcggtttaag
     7561 tagcaccttc cattctcgga tcaaatatcc taaaatgaaa acagaagttg agaagataaa
     7621 ggaagaacaa gaagaataga aagaagcgat gtataatgga tattaccttt atggatgaaa
     7681 caaaccaagt ctcagaagag aaaatgaaag aaatcgatga cttgctgcaa tttgcagcat
     7741 cttatttaaa gctgccagag gaaacagaaa tgtctgttac tttcatggac aatgcggcaa
     7801 tccaagtaat caatcgtgat taccgtggaa aagatgcacc gacagacgtc atcagttttg
     7861 ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt tgaagatgga gagaatccat
     7921 tgcctcgtgt actaggagac atcatgattt ctattgaacg tgctgctgaa caagcagaag
     7981 cttacggcca ttcatatgat cgggaacttg gctttttagc ggtacatgga ttcttgcata
     8041 tcaacgggta tgaccatatg acaccagaag atgagaaaga aatgtttggt ttacagaaag
     8101 agatcttgga tgcctatgga ctcaaaagat aaaaacgtag agaaaaacaa acatttcctg
     8161 atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt ttgaggaaga acgcaatatg
     8221 aggaaacatg ttgcgttcgg tatccttgct ttatttatgg gcgtgatttt ccagcttgat
     8281 cggatagagt ggttatggct gcttttagct gtttttctcg tttggatagt agagatcatc
     8341 aatactgttt ttgaaaatgt agtggacatg tttacggatt ttcattttca tccgattggg
     8401 aaaaaaatca aagatatggc agctggagca gttttagtga ccgcttgttt tgctgtcatc
     8461 attgggttga ttttatttgt ccctaaaata tatcatttgt tgtttaatta aaaaggagaa
     8521 cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt cggccgcccg aatgtaggaa
     8581 aatccacatt attgaaccga atcgtgggtc aaaaaattgc cattatgagt gacaaggcac
     8641 agacaactag aaataaaatc caaggagtat atactacacc agaagctcag ctgattttta
     8701 tcgatacacc aggaatccat aaaccaaagc atcgtctagg agattttatg gtagaagcag
     8761 cttacagtgc gttgaaagaa gtggatgcag tcttatttat gatcagtgct gatcaaaaac
     8821 gcggccgggg agacgatttt attattgaac gtctaaaaaa cgtgcattct ccagtttatc
     8881 tgattatcaa taaaatcgat aagatccatc cagacgagtt gctgggaatc atcgaagatt
     8941 attcgacgca gatgcctttt gcacaagtga ttccgatctc tgctacagaa ggaaacaatg
     9001 tcgaacgctt aatggatgta ttagtcagtg aaatgccaga gggcccacaa tattttccag
     9061 atgatcaagt gactgatcat cctgaatact ttatcgtttc tgaattgatc cgtgagaaag
     9121 tcttgttttt aacacgagac gaagtgcctc attctgtcgc agtcgttgtt gattcgatga
     9181 agcgaaacga gaatgataaa atccatatcc aggccacgat catcgtcgaa agagatagcc
     9241 aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa gcaagtagga acgaaagcaa
     9301 gaaaggatat cgaaaatctt ttaggagaca aagtatttct agaactttgg gtcaaagtcc
     9361 aaaaagactg gcgagacaaa aaagtctatc tgcaagactt tggctaccga aaagacgaat
     9421 actgatattc tgaacttctt ataggttaag tatagaaata aactggataa aaatccgcac
     9481 gttgagaaaa acggcggaag atcgaaacat aaataatctg gatcggcaga atctgctttt
     9541 ccagatttat tttttaggag ggagaatatg agccagcttg cagaatcgaa aggcttgatt
     9601 ttatctagcc gtaattacaa agaaaaagat aaattagtca aaatctttac ggaatcttct
     9661 ggcaaaatga tgttttatat caaaggaatc catcggcaaa atcagccatt agctccggct
     9721 ttgctcccct ttaccgaagc agtctatatc ggtaattttc gaagtgaagg tttgtcgttt
     9781 ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc aagaagatat ctttttgagc
     9841 ggttatggta cctatttgct aaatctagta gatgcagcaa tcgaagatca tcaatatgac
     9901 ccgcatttat tccaatttac gcaacaagct ctgcagcgga tggatcaagg agatgatgca
     9961 gaaatcatca cgaatatctt tgaagttcaa atactgcaac gcttcggaat cgcgccaatt
    10021 tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat ttgactattc ttccaaatct
    10081 ggcggtgtga tatgcgagaa acactggcca atggacgaac accgttatca tgcagatcct
    10141 cgcgctgttt atttcgttcg aatgttttcg gctatttctt atgataagat cagtggaatc
    10201 cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca ttgaccagtt gtatgatgaa
    10261 tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc aaatgaagtc ttgggcacac
    10321 gtattaaaga cgccagagaa aaatatgcct gaaaaagaga tacctgaaaa agaagaataa
    10381 atgaaagata caacaaaaaa atcaaaacta tttgacaaat acagggactt ccgtctatta
    10441 ttaaccgtag attaaataga tcgttcactg agaggatcta ttatataaac aagaaaactg
    10501 caaaatgaat acgtacattg accaagatca gtaaaaaaca cgtggtttag cgagttcggg
    10561 agagtggaag ccggacccaa acgatttttg aagaggcact tgcgagtacg ataaatgaag
    10621 ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa ttattcgtcc ctatgtctgt
    10681 aatcagacat agggactttt tgtcgttttc tttcctgtat tcttattgac agcaacgctt
    10741 aaaaattgaa aggggcaaaa cagatgagta aaaaattgac cgtccaagat atgattttaa
    10801 cattacaaaa attttggtca tcaaacggct gcatgctgat gcaagcttat gacacagaaa
    10861 aaggagcagg tacgatgagc ccatatacat tcttacgggc aatcggaccg gaaccatgga
    10921 atgctgctta tgtagagcca tcccgccgac cagctgacgg gcgttatgga gaaaacccaa
    10981 accgtttgta tcagcatcat caatttcaag tcgtaatgaa accttcacct gaaaatatcc
    11041 aagagcttta tttagaaagc ttgcgtcttt taggaattga tccattagaa cacgatattc
    11101 gttttgttga agacaactgg gagaacccat cgatggggtg tgccggtgtg ggttgggaag
    11161 tatggcttga tgggatggag atcacacaat tcacgtactt ccagcaagtc ggaggactac
    11221 cttgtaaacc agttacttct gagatcactt atggactaga acgtcttgct tcatatattc
    11281 aagaagttga aagtgtctat gatctggaat gggctgatgg tgtgaaatat ggcgagatct
    11341 tcaaacaacc agaatatgaa cattccaaat attcttttga agtaagtgac caagacttgc
    11401 tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg ctgtatcgat gaaaatcttg
    11461 ttcatccagc gtatgattat attttgaaat gcagccatac attcaattta ttagatgctc
    11521 gcggtgcagt atccgtaaca gaacgagcag gttatctttc ccgtatccgc aacatggctc
    11581 gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt aggattccct ttattgaata
    11641 aaaacacagc aaatccagaa aacatgaagg aggctgaata agatggcaaa agacttatta
    11701 cttgaaatcg ggctggaaga aatgcctgct catgttgtca caccaagcat gaaacaatta
    11761 gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga cttatgatac gatcgaaacg
    11821 ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca ttccagaacg tcaagcagac
    11881 agtgaagaag aagtcaaagg accagccaaa aaaattgctt tggacgcaga tggaaactgg
    11941 acgaaagcag ctgaaggatt tgtccgcgga caaggtctga cgacagatgc tatcactttt
    12001 agagaattaa aaggcgtgga atatgtctat gtaacgaaat acactcacgg caaagaagcg
    12061 aaagaagtat tgacaggttt agcagatgtc attaaaagcc tcactttccc agtaacgatg
    12121 cattgggcag aatacgattt tgaatatatc cgccctattc actggatcgt ggcattgtta
    12181 gatgacgaag taatcccgtt tgagatctta gatgtcaaaa caggcagagc ctctcgagga
    12241 catcgtttct taggcgaaga tgtgacattt gcgcatgcaa aagaatatga agaaaaattg
    12301 aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag aaatgatcgt tacacaagca
    12361 aatgaattag ccgaaaaaaa tcactgggtt attgatctag acgaagattt actagaagaa
    12421 gtcaacaatt tagtcgaata tccaactgtc ttttctggcg acttcgaaga aaaatatctt
    12481 tctgttcctg aagaagtact agtcacttct atgaaagaac atcaacgtta tttcgaggta
    12541 cggaacgatc aaggtatgct gcttcctcat tttatttctg ctcgtaatgg tgatcgggta
    12601 aaattggaaa atgtggttcg aggcaatcaa aaagtattga cggctcgttt agaagatgcg
    12661 gaattctttt ataatgaaga caagaaagct tcaatcgacc aatacgttga aaaactaaaa
    12721 actgttactt tccatgaaaa aatcggtagt atctatgaga aaatgcagcg ggtacaagtc
    12781 attgcccgta tcattggcga acaagtagga ctatctgagg aagaattgat ggatctgcaa
    12841 cgagctgctg aaatctataa atttgatcta gtaaccaaca tggtagggga atttcctgaa
    12901 ctacaaggga tcatgggcga aaaatatgct ctgctgaaag gcgagaaacc tgctgtagca
    12961 acagcgattc gtgagcatta catgcctata tccagtgaag gagagctacc tcagtctaca
    13021 gtaggttctg ttttagcgat tgcagataaa ttagatagca ttttttcttt ctttgcagtt
    13081 gggatgattc caagcgggtc aaacgatcct tatgccttac gtcgccaagc atacggtgtc
    13141 gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt ctgttctgca agaaacgatt
    13201 agtgatgtta tcagtaaaga tacggatcgt ttcggcattg gtttatcaga agggcaacaa
    13261 caggtgatcg actttatcaa aggccgtctg cgccaattat tgacaacgaa gaatattcgt
    13321 catgatgtca tcgaagctgt tttgaatgca gaacaaaaag atctgacaaa agtatttgca
    13381 gcagcacaat tgttcaagca gcatcttgca gatgaagact tcaaaccatc gatggaagct
    13441 ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg aaaaacaatc agaagtagac
    13501 cctgaactgt tcgaaaatga agcggagaaa gaactgcaca aagcagtaga ggcaatgaaa
    13561 aaaagctttg ctgaccaaac gatcaacgaa aattatgatc agcttgtaag ccttcgccca
    13621 ctgatcgaaa attattttga tcagacgatg gtcatggcag aagaaccagc agtacgtaag
    13681 aaccgattaa cgcaattaag tcaaattgct gccatggctt tgtcgctagc aagtttagat
    13741 caaatcatca caaaataatt tctaatatgt aaataaacca gagaataggc acaaaa
//
LOCUS       DAZBTH010000068        13429 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000068 DAZBTH010000000
VERSION     DAZBTH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.069"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHGNW_001914"
     CDS             complement(<1..914)
                     /locus_tag="ACHGNW_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN6926198.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHGNW_001915"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHGNW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN6926199.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHGNW_001916"
     CDS             2006..2176
                     /locus_tag="ACHGNW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926200.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHGNW_001917"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHGNW_001917"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN6926201.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHGNW_001918"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHGNW_001918"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN6926202.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHGNW_001919"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHGNW_001919"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN6926203.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHGNW_001920"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHGNW_001920"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN6926204.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHGNW_001921"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHGNW_001921"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN6926205.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHGNW_001922"
     CDS             6751..7752
                     /locus_tag="ACHGNW_001922"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN6926206.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHGNW_001923"
     CDS             7749..9110
                     /locus_tag="ACHGNW_001923"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN6926207.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHGNW_001924"
     CDS             9070..10221
                     /locus_tag="ACHGNW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6926208.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHGNW_001925"
     CDS             10196..11365
                     /locus_tag="ACHGNW_001925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6926209.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHGNW_001926"
     CDS             11540..12919
                     /locus_tag="ACHGNW_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6926210.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACHGNW_001927"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACHGNW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZBTH010000069        13399 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000069 DAZBTH010000000
VERSION     DAZBTH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.070"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACHGNW_001928"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACHGNW_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACHGNW_001929"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACHGNW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACHGNW_001930"
     CDS             complement(1144..1713)
                     /locus_tag="ACHGNW_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6926211.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACHGNW_001931"
     CDS             complement(1787..2110)
                     /locus_tag="ACHGNW_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926212.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACHGNW_001932"
     CDS             complement(2264..3076)
                     /locus_tag="ACHGNW_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926213.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACHGNW_001933"
     CDS             3403..4278
                     /locus_tag="ACHGNW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFN6926214.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACHGNW_001934"
     CDS             4292..4615
                     /locus_tag="ACHGNW_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926215.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACHGNW_001935"
     CDS             4612..5364
                     /locus_tag="ACHGNW_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN6926216.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACHGNW_001936"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACHGNW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHGNW_001937"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHGNW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN6926217.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACHGNW_001938"
     CDS             complement(9828..12014)
                     /locus_tag="ACHGNW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6926218.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHGNW_001939"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHGNW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN6926219.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACHGNW_001940"
     CDS             complement(12236..12676)
                     /locus_tag="ACHGNW_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN6926220.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACHGNW_001941"
     CDS             complement(12750..13289)
                     /locus_tag="ACHGNW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN6926221.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAZBTH010000070        13350 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000070 DAZBTH010000000
VERSION     DAZBTH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.071"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACHGNW_001942"
     CDS             1..168
                     /locus_tag="ACHGNW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN6926222.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(230..2041)
                     /locus_tag="ACHGNW_001943"
     CDS             complement(230..2041)
                     /locus_tag="ACHGNW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN6926223.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2038..2403)
                     /locus_tag="ACHGNW_001944"
     CDS             complement(2038..2403)
                     /locus_tag="ACHGNW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN6926224.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2566..2961)
                     /locus_tag="ACHGNW_001945"
     CDS             complement(2566..2961)
                     /locus_tag="ACHGNW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6926225.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2979..3941)
                     /locus_tag="ACHGNW_001946"
     CDS             complement(2979..3941)
                     /locus_tag="ACHGNW_001946"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN6926226.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3941..4153)
                     /locus_tag="ACHGNW_001947"
     CDS             complement(3941..4153)
                     /locus_tag="ACHGNW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN6926227.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4174..4872)
                     /locus_tag="ACHGNW_001948"
     CDS             complement(4174..4872)
                     /locus_tag="ACHGNW_001948"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN6926228.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4892..5434)
                     /locus_tag="ACHGNW_001949"
     CDS             complement(4892..5434)
                     /locus_tag="ACHGNW_001949"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN6926229.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5566..6570)
                     /locus_tag="ACHGNW_001950"
     CDS             complement(5566..6570)
                     /locus_tag="ACHGNW_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6926230.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6567..7556)
                     /locus_tag="ACHGNW_001951"
     CDS             complement(6567..7556)
                     /locus_tag="ACHGNW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6926231.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7553..8359)
                     /locus_tag="ACHGNW_001952"
     CDS             complement(7553..8359)
                     /locus_tag="ACHGNW_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926232.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8525..9481
                     /locus_tag="ACHGNW_001953"
     CDS             8525..9481
                     /locus_tag="ACHGNW_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6926233.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9558..10076)
                     /locus_tag="ACHGNW_001954"
     CDS             complement(9558..10076)
                     /locus_tag="ACHGNW_001954"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6926234.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10164..10313)
                     /gene="rpmG"
                     /locus_tag="ACHGNW_001955"
     CDS             complement(10164..10313)
                     /gene="rpmG"
                     /locus_tag="ACHGNW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6926235.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10541..10987
                     /locus_tag="ACHGNW_001956"
     CDS             10541..10987
                     /locus_tag="ACHGNW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN6926236.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11182..13077)
                     /gene="asnB"
                     /locus_tag="ACHGNW_001957"
     CDS             complement(11182..13077)
                     /gene="asnB"
                     /locus_tag="ACHGNW_001957"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN6926237.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4157 a         2831 c         2277 g         4085 t
ORIGIN      
        1 atgaaaaaat ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct
       61 ggtttggttg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg
      121 atcgaagaaa accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa
      181 aatacgcatg aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag
      241 tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc
      301 tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt
      361 cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag
      421 cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt
      481 ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag
      541 cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt
      601 gcttttgctg aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg
      661 ctgctaattc tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag
      721 taaaccatcc ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag
      781 cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg
      841 tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg
      901 aaaagggctc attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag
      961 ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct
     1021 caaaattttt ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca
     1081 actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat
     1141 gccattgctc caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct
     1201 tttgatttct tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg
     1261 gttccttgcc tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc
     1321 ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga
     1381 cattttcttt aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat
     1441 acagtaaaac atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat
     1501 tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct
     1561 gtaaaagata accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa
     1621 tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata
     1681 aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata attagttgct
     1741 tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca
     1801 agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca
     1861 cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac
     1921 ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat
     1981 ggagaatcct ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca
     2041 tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt
     2101 ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa
     2161 aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga
     2221 agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc
     2281 tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc
     2341 cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat
     2401 catagttgtt tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa
     2461 aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat
     2521 ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact
     2581 tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc
     2641 tgctttacgt aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata
     2701 gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt
     2761 tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg
     2821 tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc
     2881 cattactcgt agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat
     2941 attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat
     3001 gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat
     3061 tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat
     3121 tctcctgctg ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc
     3181 aagtttccgc aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac
     3241 ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct
     3301 agcattgctt tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt
     3361 accacacctg tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg
     3421 aacccttcag gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga
     3481 atcaaatttc cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca
     3541 acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg
     3601 tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga
     3661 acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt
     3721 cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa
     3781 cgatgattga ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg
     3841 gtatcgatgc tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct
     3901 gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc
     3961 ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat
     4021 gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat
     4081 agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat
     4141 ataagctctc catctttaag cacttccttc atattataca agtaaaccat atttttttaa
     4201 acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg
     4261 gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc
     4321 gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac
     4381 aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg
     4441 tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc
     4501 accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc
     4561 gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc
     4621 cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat
     4681 tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc
     4741 ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat
     4801 ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg
     4861 ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct
     4921 gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg
     4981 tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat
     5041 aaccggctct attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat
     5101 agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt
     5161 catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct
     5221 tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata
     5281 aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga
     5341 ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc
     5401 ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa
     5461 atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat
     5521 aaaaagaaga cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc
     5581 gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa
     5641 aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag
     5701 cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac
     5761 gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc
     5821 tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac
     5881 gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc
     5941 ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg
     6001 ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt
     6061 agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc
     6121 ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac
     6181 acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga
     6241 atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc
     6301 caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac
     6361 gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac
     6421 aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta
     6481 aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa
     6541 gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa
     6601 aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc
     6661 atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt
     6721 agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca
     6781 ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag
     6841 aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc
     6901 aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat
     6961 aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa
     7021 aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa
     7081 aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa
     7141 aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga
     7201 aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa
     7261 gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt caccccttgc
     7321 atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc
     7381 aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc
     7441 tgccaaaaat ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata
     7501 atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt
     7561 gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg
     7621 gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca
     7681 cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg
     7741 aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt
     7801 gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg
     7861 tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac
     7921 gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg
     7981 tagcatccaa cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt
     8041 tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt
     8101 cactagtttg tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga
     8161 tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt
     8221 tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag
     8281 gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag
     8341 ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg
     8401 ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca
     8461 gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa
     8521 agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt
     8581 actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac
     8641 ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa
     8701 cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa
     8761 acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt
     8821 aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc
     8881 tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag
     8941 caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact
     9001 aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta
     9061 tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc
     9121 tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg
     9181 aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa
     9241 aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga
     9301 ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa
     9361 gaatctaaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct
     9421 atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg
     9481 attttaacta aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca
     9541 aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat
     9601 ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata
     9661 agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg
     9721 atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca
     9781 cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa
     9841 tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata
     9901 tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa
     9961 attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac
    10021 aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta
    10081 tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca
    10141 aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg
    10201 cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt
    10261 agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc
    10321 cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat
    10381 tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat
    10441 acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg
    10501 tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga
    10561 ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc
    10621 aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca
    10681 agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa
    10741 aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc
    10801 agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa
    10861 ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca
    10921 aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga
    10981 ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga
    11041 aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag
    11101 agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt
    11161 tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat
    11221 cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt
    11281 tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg
    11341 atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg
    11401 ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca
    11461 ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt
    11521 caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg
    11581 tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt
    11641 tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc
    11701 tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg
    11761 tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc
    11821 ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc
    11881 gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat
    11941 cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc
    12001 atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa
    12061 gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg
    12121 gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag
    12181 ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga
    12241 gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa
    12301 gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc
    12361 gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc
    12421 ccaatattgc tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa
    12481 acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata
    12541 aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt
    12601 gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc
    12661 aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa
    12721 cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac
    12781 ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg
    12841 ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat
    12901 aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg
    12961 tttatcgata tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt
    13021 attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta
    13081 actttcacat cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa
    13141 taaatagcgg acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa
    13201 gtttctgttt ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga
    13261 ggctacctta ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca
    13321 gcaaaaaaag acacgaccta aagatcgtgt
//
LOCUS       DAZBTH010000071        13172 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000071 DAZBTH010000000
VERSION     DAZBTH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.073"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1800
                     /gene="yfcC"
                     /locus_tag="ACHGNW_001958"
     CDS             61..1800
                     /gene="yfcC"
                     /locus_tag="ACHGNW_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN6926238.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1957..3141
                     /gene="iadA"
                     /locus_tag="ACHGNW_001959"
     CDS             1957..3141
                     /gene="iadA"
                     /locus_tag="ACHGNW_001959"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN6926239.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3252..3455
                     /locus_tag="ACHGNW_001960"
     CDS             3252..3455
                     /locus_tag="ACHGNW_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6926240.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3556..4218)
                     /locus_tag="ACHGNW_001961"
     CDS             complement(3556..4218)
                     /locus_tag="ACHGNW_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926241.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4231..5733)
                     /locus_tag="ACHGNW_001962"
     CDS             complement(4231..5733)
                     /locus_tag="ACHGNW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926242.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6281..7960
                     /locus_tag="ACHGNW_001963"
     CDS             6281..7960
                     /locus_tag="ACHGNW_001963"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6926243.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8087..8566
                     /locus_tag="ACHGNW_001964"
     CDS             8087..8566
                     /locus_tag="ACHGNW_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN6926244.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8785..9465
                     /locus_tag="ACHGNW_001965"
     CDS             8785..9465
                     /locus_tag="ACHGNW_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN6926245.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9473..10804
                     /locus_tag="ACHGNW_001966"
     CDS             9473..10804
                     /locus_tag="ACHGNW_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN6926246.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10819..11781
                     /locus_tag="ACHGNW_001967"
     CDS             10819..11781
                     /locus_tag="ACHGNW_001967"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN6926247.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11892..12230
                     /locus_tag="ACHGNW_001968"
     CDS             11892..12230
                     /locus_tag="ACHGNW_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN6926248.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12309..13016
                     /locus_tag="ACHGNW_001969"
     CDS             12309..13016
                     /locus_tag="ACHGNW_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN6926249.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4181 a         2331 c         2896 g         3764 t
ORIGIN      
        1 catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat
       61 ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc
      121 catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt
      181 ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt
      241 actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat
      301 gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt
      361 ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa
      421 ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat
      481 gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc
      541 tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct
      601 gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc
      661 ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta
      721 gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa
      781 gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg
      841 atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg
      901 agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg
      961 acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata
     1021 tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat
     1081 ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa
     1141 ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa
     1201 ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc
     1261 attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa
     1321 tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt
     1381 cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg
     1441 atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc
     1501 caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca
     1561 atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat
     1621 cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg
     1681 ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga
     1741 ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag
     1801 tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt
     1861 cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc
     1921 tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa
     1981 gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg
     2041 gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt
     2101 gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga
     2161 ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct
     2221 ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg
     2281 ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt
     2341 tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt
     2401 gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt
     2461 tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa
     2521 gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga
     2581 atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca
     2641 ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact
     2701 gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca
     2761 ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga
     2821 caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt
     2881 gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa
     2941 atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga
     3001 aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt
     3061 gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg
     3121 actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc
     3181 tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag
     3241 gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg
     3301 gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga
     3361 aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca
     3421 aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca
     3481 agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga
     3541 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
     3601 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
     3661 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
     3721 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
     3781 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
     3841 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
     3901 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
     3961 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
     4021 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
     4081 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
     4141 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
     4201 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
     4261 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
     4321 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
     4381 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
     4441 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
     4501 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     4561 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     4621 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     4681 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     4741 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     4801 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     4861 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     4921 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     4981 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     5041 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     5101 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     5161 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     5221 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     5281 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     5341 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     5401 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     5461 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     5521 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     5581 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     5641 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     5701 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     5761 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     5821 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     5881 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     5941 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     6001 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     6061 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     6121 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     6181 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     6241 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     6301 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     6361 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     6421 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     6481 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     6541 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     6601 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     6661 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     6721 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     6781 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     6841 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     6901 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     6961 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     7021 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     7081 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     7141 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     7201 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     7261 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     7321 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     7381 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     7441 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     7501 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     7561 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     7621 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     7681 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     7741 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     7801 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     7861 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     7921 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     7981 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     8041 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     8101 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     8161 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     8221 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     8281 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     8341 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     8401 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     8461 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     8521 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     8581 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     8641 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     8701 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     8761 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     8821 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     8881 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     8941 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     9001 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     9061 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     9121 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     9181 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     9241 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     9301 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     9361 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     9421 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     9481 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     9541 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     9601 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     9661 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     9721 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     9781 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     9841 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     9901 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     9961 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
    10021 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
    10081 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
    10141 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
    10201 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
    10261 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
    10321 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
    10381 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
    10441 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
    10501 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
    10561 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
    10621 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
    10681 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
    10741 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
    10801 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
    10861 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
    10921 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
    10981 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
    11041 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
    11101 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
    11161 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
    11221 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
    11281 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
    11341 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
    11401 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
    11461 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
    11521 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
    11581 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
    11641 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
    11701 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
    11761 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
    11821 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
    11881 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
    11941 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
    12001 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
    12061 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
    12121 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
    12181 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
    12241 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
    12301 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
    12361 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
    12421 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
    12481 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
    12541 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
    12601 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
    12661 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
    12721 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
    12781 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
    12841 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
    12901 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
    12961 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
    13021 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
    13081 ccatttattt ggaataaggg ctctttgtca ataaggactg atgggttgta caaaatcaaa
    13141 tctgggtcag aatgaacctc attctggctc ag
//
LOCUS       DAZBTH010000072        12943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000072 DAZBTH010000000
VERSION     DAZBTH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.074"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACHGNW_001970"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACHGNW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN6926250.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACHGNW_001971"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACHGNW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN6926251.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACHGNW_001972"
     CDS             complement(3218..5533)
                     /locus_tag="ACHGNW_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6926252.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACHGNW_001973"
     CDS             complement(5718..6578)
                     /locus_tag="ACHGNW_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN6926253.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACHGNW_001974"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACHGNW_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACHGNW_001975"
     CDS             7436..8197
                     /locus_tag="ACHGNW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926254.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACHGNW_001976"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACHGNW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6926255.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACHGNW_001977"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACHGNW_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6926256.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACHGNW_001978"
     CDS             12557..12913
                     /locus_tag="ACHGNW_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6926257.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAZBTH010000073        12903 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000073 DAZBTH010000000
VERSION     DAZBTH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.075"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHGNW_001979"
     CDS             133..3660
                     /locus_tag="ACHGNW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN6926258.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHGNW_001980"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHGNW_001980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN6926259.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHGNW_001981"
     CDS             7448..7789
                     /locus_tag="ACHGNW_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6926260.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHGNW_001982"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHGNW_001982"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN6926261.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHGNW_001983"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHGNW_001983"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN6926262.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHGNW_001984"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHGNW_001984"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN6926263.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4380 a         2242 c         2738 g         3543 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tct
//
LOCUS       DAZBTH010000074        12734 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000074 DAZBTH010000000
VERSION     DAZBTH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.076"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHGNW_001985"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHGNW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6926264.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHGNW_001986"
     CDS             complement(322..891)
                     /locus_tag="ACHGNW_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN6926265.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHGNW_001987"
     CDS             1077..1979
                     /locus_tag="ACHGNW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6926266.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHGNW_001988"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHGNW_001988"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN6926267.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHGNW_001989"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHGNW_001989"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN6926268.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHGNW_001990"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHGNW_001990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN6926269.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHGNW_001991"
     CDS             8547..8732
                     /locus_tag="ACHGNW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN6926270.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHGNW_001992"
     CDS             complement(8794..9990)
                     /locus_tag="ACHGNW_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6926271.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHGNW_001993"
     CDS             complement(10160..11596)
                     /locus_tag="ACHGNW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6926272.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHGNW_001994"
     CDS             11889..12698
                     /locus_tag="ACHGNW_001994"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6926273.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZBTH010000075        12446 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000075 DAZBTH010000000
VERSION     DAZBTH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.077"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(393..980)
                     /locus_tag="ACHGNW_001995"
     CDS             complement(393..980)
                     /locus_tag="ACHGNW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926274.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1155..2477
                     /locus_tag="ACHGNW_001996"
     CDS             1155..2477
                     /locus_tag="ACHGNW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6926275.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2632..3408
                     /locus_tag="ACHGNW_001997"
     CDS             2632..3408
                     /locus_tag="ACHGNW_001997"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6926276.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3401..3970
                     /gene="rnmV"
                     /locus_tag="ACHGNW_001998"
     CDS             3401..3970
                     /gene="rnmV"
                     /locus_tag="ACHGNW_001998"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN6926277.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3963..4847
                     /gene="rsmA"
                     /locus_tag="ACHGNW_001999"
     CDS             3963..4847
                     /gene="rsmA"
                     /locus_tag="ACHGNW_001999"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN6926278.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5298..5927
                     /locus_tag="ACHGNW_002000"
     CDS             5298..5927
                     /locus_tag="ACHGNW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926279.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6051..7160)
                     /locus_tag="ACHGNW_002001"
     CDS             complement(6051..7160)
                     /locus_tag="ACHGNW_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926280.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7369..7791
                     /gene="mgsA"
                     /locus_tag="ACHGNW_002002"
     CDS             7369..7791
                     /gene="mgsA"
                     /locus_tag="ACHGNW_002002"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN6926281.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7978..8076
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            8114..8650
                     /locus_tag="ACHGNW_002003"
     CDS             8114..8650
                     /locus_tag="ACHGNW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6926282.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8690..10453)
                     /locus_tag="ACHGNW_002004"
     CDS             complement(8690..10453)
                     /locus_tag="ACHGNW_002004"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926283.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10456..12171)
                     /locus_tag="ACHGNW_002005"
     CDS             complement(10456..12171)
                     /locus_tag="ACHGNW_002005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926284.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3913 a         2274 c         2514 g         3745 t
ORIGIN      
        1 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
       61 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
      121 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
      181 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
      241 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
      301 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
      361 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
      421 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
      481 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
      541 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
      601 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
      661 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
      721 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
      781 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
      841 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
      901 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
      961 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
     1021 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
     1081 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
     1141 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
     1201 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
     1261 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
     1321 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
     1381 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
     1441 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
     1501 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
     1561 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
     1621 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
     1681 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
     1741 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
     1801 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
     1861 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
     1921 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
     1981 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
     2041 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
     2101 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
     2161 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
     2221 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
     2281 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
     2341 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
     2401 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
     2461 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
     2521 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
     2581 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
     2641 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
     2701 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
     2761 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
     2821 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
     2881 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
     2941 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
     3001 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
     3061 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
     3121 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
     3181 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
     3241 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
     3301 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
     3361 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
     3421 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
     3481 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
     3541 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
     3601 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
     3661 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
     3721 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
     3781 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
     3841 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
     3901 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
     3961 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
     4021 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
     4081 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
     4141 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
     4201 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
     4261 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
     4321 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
     4381 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
     4441 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
     4501 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
     4561 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
     4621 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
     4681 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
     4741 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
     4801 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
     4861 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
     4921 ttcctcatgt ttttcgtttg tttgggcttg ttaccgcaga tgcaagcttt tgaacaccgt
     4981 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
     5041 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
     5101 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
     5161 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
     5221 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
     5281 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
     5341 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
     5401 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
     5461 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
     5521 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
     5581 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
     5641 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
     5701 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
     5761 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
     5821 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
     5881 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
     5941 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
     6001 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
     6061 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
     6121 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
     6181 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
     6241 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
     6301 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
     6361 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
     6421 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
     6481 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
     6541 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
     6601 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
     6661 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
     6721 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
     6781 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
     6841 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
     6901 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
     6961 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
     7021 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
     7081 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
     7141 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
     7201 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
     7261 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
     7321 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
     7381 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
     7441 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
     7501 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
     7561 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
     7621 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
     7681 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
     7741 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
     7801 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
     7861 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
     7921 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
     7981 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
     8041 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
     8101 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
     8161 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
     8221 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
     8281 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
     8341 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
     8401 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
     8461 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
     8521 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
     8581 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
     8641 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
     8701 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
     8761 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
     8821 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
     8881 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
     8941 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
     9001 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
     9061 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
     9121 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
     9181 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
     9241 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
     9301 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
     9361 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
     9421 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
     9481 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
     9541 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
     9601 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
     9661 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
     9721 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
     9781 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
     9841 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
     9901 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
     9961 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    10021 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    10081 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    10141 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    10201 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    10261 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    10321 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    10381 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    10441 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    10501 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    10561 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    10621 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    10681 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    10741 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    10801 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    10861 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    10921 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    10981 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    11041 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    11101 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    11161 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    11221 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    11281 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    11341 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    11401 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    11461 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    11521 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    11581 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    11641 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    11701 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    11761 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    11821 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    11881 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    11941 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    12001 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    12061 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    12121 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    12181 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
    12241 tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt
    12301 agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt
    12361 cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata
    12421 tatcaagtaa atgtttatag atgaaa
//
LOCUS       DAZBTH010000076        12308 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000076 DAZBTH010000000
VERSION     DAZBTH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.078"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHGNW_002006"
     CDS             288..695
                     /locus_tag="ACHGNW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926285.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHGNW_002007"
     CDS             709..1110
                     /locus_tag="ACHGNW_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926286.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACHGNW_002008"
     CDS             1112..1483
                     /locus_tag="ACHGNW_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926287.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACHGNW_002009"
     CDS             complement(1519..1818)
                     /locus_tag="ACHGNW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6926288.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="ACHGNW_002010"
     CDS             complement(1824..2054)
                     /locus_tag="ACHGNW_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926289.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2671..3333
                     /locus_tag="ACHGNW_002011"
     CDS             2671..3333
                     /locus_tag="ACHGNW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926290.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3412..6637
                     /gene="fss3"
                     /locus_tag="ACHGNW_002012"
                     /pseudo
     CDS             3412..6637
                     /gene="fss3"
                     /locus_tag="ACHGNW_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6751..7065
                     /locus_tag="ACHGNW_002013"
     CDS             6751..7065
                     /locus_tag="ACHGNW_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6926291.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7078..7452
                     /locus_tag="ACHGNW_002014"
     CDS             7078..7452
                     /locus_tag="ACHGNW_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6926292.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7462..7806
                     /locus_tag="ACHGNW_002015"
     CDS             7462..7806
                     /locus_tag="ACHGNW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN6926293.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7877..9229
                     /locus_tag="ACHGNW_002016"
     CDS             7877..9229
                     /locus_tag="ACHGNW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN6926294.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9289..10431
                     /locus_tag="ACHGNW_002017"
     CDS             9289..10431
                     /locus_tag="ACHGNW_002017"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFN6926295.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10456..10710
                     /locus_tag="ACHGNW_002018"
     CDS             10456..10710
                     /locus_tag="ACHGNW_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926296.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10966..12150
                     /gene="mobT"
                     /locus_tag="ACHGNW_002019"
     CDS             10966..12150
                     /gene="mobT"
                     /locus_tag="ACHGNW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN6926297.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12147..12284
                     /locus_tag="ACHGNW_002020"
     CDS             12147..12284
                     /locus_tag="ACHGNW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN6926298.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3477 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgtagga tcgaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaattta
     1981 tcttttttgc tgctttgctc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aagtcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt ctcgttgtaa
     2101 agcgatattt gaggggcaaa aagggggcaa aaatacgttt atctttgata tatctcagat
     2161 tgaacatagt gtttattata tgtcggtatt gttgatttat agctgttttg agggtattag
     2221 atacataata gaatatagtg gttttgttta cgtgcattac acggaaaagc tgctcgtatc
     2281 aaagaaatca gaagataaac acggaaaact tccttggaaa tctgaaattt gtgaaaagtc
     2341 cttgtatatc aagggctttt tcttttttct ctatcacagt tttaagccat ttttggctgt
     2401 tttggaccat ttttcaatca tattccctta aaaattgtcc tagattgtcc gacattgact
     2461 atcagtgtcg tcaaaatagg gcaaaattta gtagtcagct tgacgacaaa tataatacga
     2521 aaaataccaa aacatttacc gaatttagca acttttgaac ccaagtttct caaagggtca
     2581 gctattagca aaaaagacaa aacatgtact tttctactaa aaagttgatg tgataggctt
     2641 ctatctaaat aactattagg agtttttatc gtgaagcaaa atgatggacg tatcatttgg
     2701 aaaaatcaaa gtgagttaaa actgatttta acaattaatg aattcatcga aaaacacgga
     2761 attacatcta gccgtcaata ccagaaaaag ctatcagaaa atccaaattc tgcaccaagt
     2821 atgtggttca ttaacaaaaa atatggatca tgggagaatt tattgattag tataggacga
     2881 gaaaatactg ggtatggaaa atgggctaga atgtcagaac aagaattatt ggaaatagta
     2941 gaagccttta ttaagtgcga gaagataacc tctcaacgaa tgtacgaaca aaaatcagta
     3001 gggaagaata ttccttcact tagtaccatt aaaaagatgt taggagatat acgtccactt
     3061 tttaaagaaa aaaatgatgg atctcgattt actgatttcg aactgctgtt agaactaaaa
     3121 aatgaaataa tccgattaaa actacaggat gatttatcaa tgacaaagtt tcgaaaacta
     3181 gttcaatccc caaaactacc atcagttgat acaattatga aaagaacgaa caaaaattgg
     3241 gaggaattga tggcagagat tggatttgat tatcgaagaa tcaaaattta taagcagaga
     3301 aacaacctat ccaagacaaa gaaaactaaa tagtatcact ctaaatcaga cgttcaaaat
     3361 taaaatttgg acgtcttttt tattacccaa aaacgaaagg agatttattt tatgaaaaag
     3421 ccaagattta agaattggcg tttgctcgca acactgagtc tattatgcca aacaattggt
     3481 ggatcgcttg gcccaacgat tgcctttgct gatgaaatta ctcatccaca aacggtaact
     3541 gtggaattgg atttggctca ccagtatgct gtcgaaggta catttagcga tggtcgtccc
     3601 atgtcagaag ttaccgttcc gcactatgca gtttacaatg gtgtgaagca agatattttc
     3661 tgtattgagc caggggtgcc gatttacaat gagttcactc ctggttatga gaaaaatcca
     3721 ttgcctgata tgtcggagaa agcgaaatta gtttccgttt tatggaagga agctggtacg
     3781 gatgtggata cacatattgt ggcccagaag atgatttggc aagaagttaa tggctacgct
     3841 tcactcaatt aaacgttctg atggtagtgc agtaaatatc gcagccattg agtcaaaaat
     3901 caatcaagcc attgcggatt atcaaaagaa accaagcttc cataactcca cagtgaaaac
     3961 cgtgttaggt caatcgacta ctgtgacaga tacgaacggc ttgaatttat cggagtttga
     4021 tgaagttgtc gagaatatcg caaatattga ttatcgtgtg aacggcaatc aactagtgat
     4081 tactccaaat gccaattcaa aagagagtgg tgaattaacc ctaaagaagt cagctaatac
     4141 aggaacaccg gtagcttata aaatggctgg tcaacaaact ttgatggcgg gggcaattga
     4201 taagcccaat acctacacaa tcaaaattga tgtggaaact gagggttctt tgaagattaa
     4261 aaaagtcgat aaagaaacag gaaatgttgt accgggaacc gttttccatt tagactttgg
     4321 aaaaacatta cctgcaaaag acgtgaccac cgataaagaa ggcattgcga cattggatga
     4381 gattcctcat ggcgcgaaag taaccattac tgaaaaatca gtgccagctc cttatacgat
     4441 tgacactaca ccaatcaccg caaccattaa atctggtgag actatttctg tgacttcaaa
     4501 aaatactcgc gaaaaaggtc aaatcatttt gaataaaatg ggggtggaaa caggaactga
     4561 tctttggaac gacaaatact cattagccgg aaatacgttt gccattcgca aagacagtcc
     4621 aaccggtgaa attgtccaag aaatgaccac ggatgaaaaa ggtcatgcgg aaacaccaaa
     4681 agagattgcc aatgcgttgg aattgggaac ctattatgtg accgaaacca aagctagtaa
     4741 tggctttgtg aataccttta aaccagtaaa agtcgaattg aactatgtca atcaaaccgt
     4801 ggctcttgtt accagtaacg taaaagggca aaaccaagaa atcactggtg aaaccacttt
     4861 gacaaaagaa gacaaagata ccggtaatga aactcaaggt aaagctgagt ttaaaggagc
     4921 tgaatatact ctctttactg caaaagatgg tcaagcggtc aaatggagtg aggcttttaa
     4981 accagagtta gtgaagggaa cgaaagcttc tgatgaaacg gtgactttga ctttagatga
     5041 aaagaatcaa gttgctgtta aacacttagc gattaatgag tattactggc aagaaaccaa
     5101 agcacctgaa ggatatacct tggatgaaac aaagtaccct gtttccatca aaaaggtgga
     5161 tgataacgaa aaaaatgccg tgattacccg agatgttacg gcaaaagaac aagtcattcg
     5221 ctttggcttt gatttcttta aatttgctgg atcagctgct gggactgccg aaactggctt
     5281 caatgatctg acctttaaag tatcgccatt ggaaggaacc agtgagatta caggtgccga
     5341 agatgaagca attacagctt ataacgagca actaggtttt gatggctatg gcaagtttga
     5401 aaatcttcct tatggggatt atttacttga agaagtagag gctccagaag gatttcaaaa
     5461 aattacgcca ttagaaatcc gttctacttt taaagaaaat aaagcggatt acactaagag
     5521 tgaatacatt tttacgatta ccgaaaaagg acaaaaacaa ccaattaaaa cggtgaccgt
     5581 tccttacgag aaactgacga ataaagcatt ttctgttagc ttgaatcgtt tgatgctcta
     5641 tgatttacct gaagaagaag atagcttaac ttctcttggg acttggaaag acggaggcaa
     5701 agaattgaca gaccttgatt ctaccgagct agttgataaa ttgagctata acttgcatga
     5761 aatcaaagaa gactggtatg tggtagcgca agccattgat gtggaagcga caaaagctgc
     5821 ccaagaaaaa gatgaaaaag ccaaaccagt ggtaattgcc gaaacaaccg caacgttggc
     5881 gaataaagag aaaacaggaa cttggaaaat tttgcataaa ttaaccgcag aacaagtttt
     5941 ggataaaacc atcgtcttat tcaactatgt gtatgaaaac aagaaaccct atgaagcagg
     6001 tgatgatcca gtagcgaaag atgctagctt gaacaatcaa gcccaaactg tcaattgtac
     6061 ggtggaacgc catgtttcca tccaaacaaa agcccaccta gaagatggtt cgcaaacctt
     6121 tactcatggg gatgtggtgg atatgttcga tgatgtgtcg attacccatg atgtactgga
     6181 tggctcaaaa gaagcttttg aaacaattct gtatgcatta cttccggata atacgaccaa
     6241 agaaatttgg aaatctggca aaattgatta tgaagtgaat gacaaagaat tcactaaaac
     6301 cgtacttgcg gaaaaagtgg acacaagtaa atatccagaa ggtacaagtt ttaccttcaa
     6361 agaaattaac tatggcaaag acggaaacat caatgggaaa cacaatgagg atctaaaaga
     6421 aaaaactcaa actttaacac ctaaaaaagt tccgacaaca ccaagtgcac cagaacaacc
     6481 ggaaatacca actgttccaa gtgactctca agaatctagt cctacagtaa agacattccc
     6541 acaaacaggg gagaaaaatt ctaatgttct gctgtttatt gggtttacct tgatctttgc
     6601 gactgctggg tattatttct ggaatcgccg aaactaaggt gatgtgatga aacaactaaa
     6661 taggaaaaac caacgtgcgc gaccgcctcc taaaaaaagt ggtcgcgcta tttttgtatc
     6721 caaaactaaa aatgaaaaga ggaaaagaag atggaattga aatttgttgt gccagatatg
     6781 gccgaaacct ttggaaagat aaggtatgcc ggcgaaggcg aagttttaac tgaaggatat
     6841 gggcggaata ctacagtaat tggtcgtagt tatcatttgt attccagtaa gcaacgagcc
     6901 gatgatattg aagtggtggt agctgcggaa gccggcgaaa aggattttga tcaagatcaa
     6961 ccactaaaag ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg
     7021 tttaccgact acgtgttgta tgtcgatgat ttagtgaaag cataggagga aaagaagatg
     7081 agattaacag aaggtattgt tgtagattca ggactaacgt ttgggaaatt acgattttcc
     7141 gcattacgcc gagaagtacg gaagcaaaat gaggatggaa cggttagtaa cgaggtaaaa
     7201 gaacggactt ataatttgaa atcctctgcg caaggtcgaa tgatccaagt tagtatccca
     7261 gcaaatgtac ccttacgcga gtttgcctat gacgcagagg tagagttggt caatccaatc
     7321 atggatacgg tcgccaatta tgttttccga gaaggaacta ctgtcaattg gttcattaag
     7381 gccgatgatt tagtcttgaa acgacaacca gatcagggaa attctacaaa ccaaaatgaa
     7441 ggaaagaaat aggtgaagcg tatggaaatt aatgttgaac atattttaga ttgtctcgat
     7501 caatatggaa aaggtgagct aacggaggaa caactcacta aagcattaac ctatgatgaa
     7561 aaaatgtttc tgattatgca tcaagggtta ctggaagtag gtaacgatgc cgaagaagat
     7621 tttgatgtac tgaattggtt aggggaagaa gagtccttct ttatggttat cgaagtaaat
     7681 gaaagtctat gtcgagaagc cgaaaccgta ttggaagaaa ttggtgtgga aatgccggat
     7741 gccattgaag tttttttaaa gcaactagtg gaaacgaaac aactccctat agcagtaaat
     7801 gactagcata actgcttgaa attggatcaa tgaaatccta tgaagctttt ttcgattgga
     7861 ggtggaaacc ataaaaatgt acaaagggca tcgtatacgg gcaggagatc agcacttggt
     7921 ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta
     7981 ttttcaagag cttagacaat ttgatatttc taagctatca ctttctacta tagaaatcgt
     8041 tcgttctata aaagatttaa tctatttgct aggaagtctc gttctttcag gaagcacgat
     8101 gttgctttat atacactttt ttcaggatca ttggcgcagt ttgtggcatc gacaaaagct
     8161 agcccggatg attctagaaa atcattggta cgaagtaaaa caaactcaga gtgaaggatt
     8221 tttcaaagac ctcaatagta gtcgaaccaa agagacaatc agttacttcc ccaaaatcta
     8281 ttaccgtatg aaggatggtt tactttctat tcgcgttcaa atttcactgg gaaaatatca
     8341 agatcagctt ttaaagctgg aaaagaagtt agaaagtggt ctatattgtg aactagtaga
     8401 aaaggaactc aaagactcct atgtggagta cactttactg tatgatataa ttgccaatcg
     8461 aattgggata gacgaagtag tggcagaaag tggggctttg cgattgatga aaaatcaagt
     8521 atgggcttat gattctttac ctcatatgtt aatcgctggc ggcacaggtg gtgggaagac
     8581 ttacttttta ctcactatca tcgaggcttt attaaaatct gatgccgagc tttttgttct
     8641 tgatcccaaa aatgcggact tagccgattt aggtacggta atgcctcatg tctattccca
     8701 aaaggaggaa atttctgctt gtgtggaaga tttctatgag cgcatgatga ctcgtagcaa
     8761 ggcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtttc ttggacttcc
     8821 accaaacttt ttaatctttg atgaatacgt ggtttatatg gaaatgttaa cgacgaagga
     8881 aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttaggtcgtc aatctggttt
     8941 ctttctgatt ctagcttgtc aaagaccgga tgcgaaatat ttaggagacg ggattcgcga
     9001 tcaatttaat ttccgggtgg ctttgggtcg tatgagtgaa ctgggatatt ccatgatgtt
     9061 tggcgaggtt gataaaaaat tctttatgaa gcgtatcaaa ggtcgaggct atgtagatac
     9121 gggagggagt gtgattagtg agttttatac gccacttgta ccaaaaggat atgatttctt
     9181 aagggaaatt agcgacattg gtggattaaa cgttaatacg gaaggataaa ataatagtat
     9241 gtagcctagg agtgtcaaga atcctgggct ttttttacga ggtgatttat gacattttta
     9301 gatttattcg ctgggattgg tggcttttgc ttaggtatgg aacaagcggg tcaccagtgt
     9361 atcggctttt gtgaaattga tgattttgct cgccagagtt ataaggcaat ccatgataca
     9421 agtaaggagg tggaaatgca tgacatcaca agtgtatcag acgagtttat tcaatctctc
     9481 ggaccagtgg atatcctttg cggtggattt ccgtgccaag ctttttcaat tgcgggaaag
     9541 cggcaaggat tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcgccgct
     9601 attctccaac ctaagttttt attccttgag aacgtccggg gattactcaa tcacgaagga
     9661 ggggctacgt tcgagacgat cctccgaacg ctggatggat tggggtatga tgtggaatgg
     9721 caagtgctta actcaaaggc ctacgttccc caaaaccgtg agcggatctt ccttatcgga
     9781 cattctcgag acgcatgtac cgaacaagta tttcctatca ttggatcgtc tccaacatct
     9841 gatcaaaaca tcagaaactt attaaatatc aacccttcca atcgaggaat ggggggacaa
     9901 gtctatgggt cagatgacgt agcgcctacc ttgaccggtg atgaaggaat caaaatcgca
     9961 ttgccggtag atgatggaat ttctgtagcg ggtatgttac tgggaaattt tgaacaaggc
    10021 aatcgggttt atgagataac tggaacagcg cctactttgt caacgaaaca aggcggaacg
    10081 aagatcatgc ttcggaaaaa cgaaacagaa tatcaagagg tgaagccagg gaacagtgtg
    10141 aacctggctt ttcctaattc cacaagtcgt cgaggccgtc tgggaaaaca aagtgtgcat
    10201 accttattaa caggagacca acaagcagtc gtcacggatc agtatcaaat tcgaaagtta
    10261 acacctaggg agtgctggcg gcttcagggc tttccggact gggcttttga tcgagcttct
    10321 caagtcaatt cagatagtca gttgtataag caggctggaa attctgtaac agttccggtc
    10381 atttttgata ttgctagaag attaaaggag gtaaaaacag atgatttatg atgagttgat
    10441 tggggaatct actgggtgat tggcaagatt cagtcagatc cggaattgga agaagaactg
    10501 taccggctca attttgatat tcgcaaaaac ggggtgaaag tccctgatga tccgtatgta
    10561 attgatgagg aaacggatgc tcgaatggag ctgaatcaag tcatcagtga attggaacga
    10621 attgcggact ttgctaaaga acctgatatc cgccaatact tgtttgagat aaaagcagag
    10681 ttggaaattg agggtattac cgcggagtaa cggccaaata aaccactgat aggcactgac
    10741 cgcgggggat ttgcgaagca gaaatcctcc gtggtgctgt gcctgtttat ggacggcgtt
    10801 agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac acaaaaaata
    10861 agcaagcaac gataaggaat ggctgtccta tcaaggataa taagcaattt gtgtagatgt
    10921 taactgattt gaaaaagtta acaccttaaa tttggaggtg agaacttggc acaaaggaat
    10981 ttagattatc ggctattaaa agaccggcgt aatgaatatg gcgtttcaca aaataaatta
    11041 gctacgactt gtggacttag tcgcccgtac ttaaatcaga tagaaaatgg tggcgtaacc
    11101 gcatcgacaa aaaccatgag gaaaattttt gaccaactgg aaagtttcaa tcccgagtta
    11161 cctttaacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga tgcgaggaaa
    11221 atcattcaag agattctcca tttgaaattt gattatatgc tccacgaaga ttacgccttt
    11281 tactcctatc aagaacaata tgttatggga gatattgtgg tgatgttgtc acatgaagaa
    11341 gataaaggcg tccttttaga attaaagggt cgtggttgtc ggcagtttga aactttttta
    11401 ctcgcccaaa agcgtagctg gtatgacttt ttcgaagatt gtctaaaagc cggtggcgtg
    11461 atgaaacgct tagacctggc aatcaatgat cgagttggcc tgttagatat tcctgattta
    11521 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa aagctatcgt
    11581 tccggggaac tgttgaaacc tgatgataaa gatggtatgg gcaatacttt gtatattggc
    11641 agtcttaaga gtgaggtcta tttctgtttg tacgaaaaag actatgagca atatatcaag
    11701 ttagggattc ctttagacca gaccgaaacg aaaaatcgtt ttgaaatccg actgaaaaat
    11761 gatcgagctt atcatgcaat tcaagatttg ttaaaaggtc gcagtattga aagtaccaca
    11821 ttttccatta ttaaccggta cttgcgattt gcggataagg tggaaggcaa aaggcgaact
    11881 aactggccct tgaatgaaca atgggggcgc ttcattggac ggaatcagaa ggaaattcaa
    11941 ttaacttctg aaccgaagcc ttataccatt gaacgaacgt tgaactggct tggacgacaa
    12001 gtcgccccta cgtggaagat ggccaaggag ttggatcggt tgaatcagac aacgtacatt
    12061 caggatatgg taaataatac tcgactatca gaccggcaca aaaagatttt ggagcaacaa
    12121 agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtgatggaa attgtgaaag
    12181 atattttatt ggtgtttggt ggcagtatgc ttggtgttac tgtgatgtgc ttgatgcatg
    12241 caagtgcagc ggcggaccga gcaatggaaa agaaaggaaa gtgatgaaat gaatttcggt
    12301 caaaattt
//
LOCUS       DAZBTH010000077        12238 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000077 DAZBTH010000000
VERSION     DAZBTH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.079"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..607)
                     /gene="frr"
                     /locus_tag="ACHGNW_002021"
     CDS             complement(50..607)
                     /gene="frr"
                     /locus_tag="ACHGNW_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN6926299.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(610..1332)
                     /gene="pyrH"
                     /locus_tag="ACHGNW_002022"
     CDS             complement(610..1332)
                     /gene="pyrH"
                     /locus_tag="ACHGNW_002022"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN6926300.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1468..2349)
                     /gene="tsf"
                     /locus_tag="ACHGNW_002023"
     CDS             complement(1468..2349)
                     /gene="tsf"
                     /locus_tag="ACHGNW_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN6926301.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2448..3230)
                     /gene="rpsB"
                     /locus_tag="ACHGNW_002024"
     CDS             complement(2448..3230)
                     /gene="rpsB"
                     /locus_tag="ACHGNW_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN6926302.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3589..4068
                     /locus_tag="ACHGNW_002025"
     CDS             3589..4068
                     /locus_tag="ACHGNW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6926303.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4285..5376
                     /gene="phnW"
                     /locus_tag="ACHGNW_002026"
     CDS             4285..5376
                     /gene="phnW"
                     /locus_tag="ACHGNW_002026"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFN6926304.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5369..5497
                     /locus_tag="ACHGNW_002027"
     CDS             5369..5497
                     /locus_tag="ACHGNW_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN6926305.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5501..6046
                     /locus_tag="ACHGNW_002028"
     CDS             5501..6046
                     /locus_tag="ACHGNW_002028"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFN6926306.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6500..8191)
                     /gene="argS"
                     /locus_tag="ACHGNW_002029"
     CDS             complement(6500..8191)
                     /gene="argS"
                     /locus_tag="ACHGNW_002029"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN6926307.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8611..9558)
                     /gene="arcC"
                     /locus_tag="ACHGNW_002030"
     CDS             complement(8611..9558)
                     /gene="arcC"
                     /locus_tag="ACHGNW_002030"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN6926308.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9673..10692)
                     /gene="argF"
                     /locus_tag="ACHGNW_002031"
     CDS             complement(9673..10692)
                     /gene="argF"
                     /locus_tag="ACHGNW_002031"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN6926309.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10783..12012)
                     /gene="arcA"
                     /locus_tag="ACHGNW_002032"
     CDS             complement(10783..12012)
                     /gene="arcA"
                     /locus_tag="ACHGNW_002032"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN6926310.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3355 a         2533 c         2205 g         4145 t
ORIGIN      
        1 taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat taaacttcca
       61 ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg ttatctgtca
      121 atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca ccattttttt
      181 gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg atttttgcat
      241 tttctgcttc ttttttaaca tctttagcta attcttttct gcgttcttct gtcagttgcg
      301 ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata tcacttgcca
      361 taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc attaaaacac
      421 gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca ccgtagtagt
      481 tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt tgcccaagtt
      541 cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc ataattgcat
      601 ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga ttgcacggcg
      661 aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat ccatgcttag
      721 tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca gatgagtcag
      781 ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa caccgtctac
      841 attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta atgctgctgt
      901 tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga tccgtccttt
      961 ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac gcatttcaat
     1021 cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta aagctaaggc
     1081 attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca tgcccatttg
     1141 tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca tttcaacgcc
     1201 cagctcgtga acttctttga tttcttggat gatttctttg attactggtg gtttgattcc
     1261 aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac gctgatattt
     1321 cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat acttagatga
     1381 tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc gcacaaaccg
     1441 tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa cttcttctac
     1501 aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga atgttttaac
     1561 agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt taacgaatgg
     1621 ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta ccattttttc
     1681 aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag ttttttcatg
     1741 ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga tagctgctac
     1801 gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa cagtcaatac
     1861 ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt ctttttctac
     1921 aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt cgatcaagtc
     1981 agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt ctgctggttt
     2041 gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt cgtttttaga
     2101 aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt tcacagcaac
     2161 tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg ccattccttt
     2221 ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta atgctttttt
     2281 cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt tagctgtaac
     2341 gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg cttcaaagga
     2401 gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta ttctgctgaa
     2461 gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt gttttcttct
     2521 gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa agcatcagcc
     2581 atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg gattactacg
     2641 tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat atttaatttg
     2701 tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat tacatctggg
     2761 attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg ttctttgttc
     2821 aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc cattgcattg
     2881 atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa tgttccgcct
     2941 aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt gattgcttct
     3001 tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc aacgctcttc
     3061 atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat gatgtagatt
     3121 ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg agtttggtga
     3181 ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat gtttgtttcc
     3241 tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa ctttttcaag
     3301 cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac cctcagctat
     3361 tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca acattcaata
     3421 gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt gtttctttat
     3481 tatccttttt ccataaaata attttaggta taaaagacga atattattgg gcaatccttc
     3541 ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat gcgcaaagct
     3601 caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac gcaagaagaa
     3661 ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat ttctcgagat
     3721 atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg cttcgtattg
     3781 tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct ggttcaaatg
     3841 attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt caataccttg
     3901 ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc ctctattgtc
     3961 agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga ggacgcaaaa
     4021 cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc agagcacgta
     4081 ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga ataaaccttt
     4141 acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt gattgaatca
     4201 caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt tgtgtatttc
     4261 aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc attgacgact
     4321 tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca agaatacaaa
     4381 gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc ggaaggaata
     4441 tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt attaagttct
     4501 gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag acaacgaatg
     4561 atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc ggaacatcaa
     4621 atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat ctccgcacta
     4681 gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga gattgctgct
     4741 gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt tggtgggata
     4801 ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa caaatgtatt
     4861 caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga aaaacgaaaa
     4921 aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat ggaaaacgag
     4981 aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca acaagcgctg
     5041 aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct cgccaataat
     5101 catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca agaatatcag
     5161 gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt taatcatttt
     5221 tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag cgatcatgaa
     5281 tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga actgacagca
     5341 attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg attgggcagg
     5401 aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg cttttctaga
     5461 aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat taaaatggga
     5521 tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag ctatttatgg
     5581 acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac attatctgtt
     5641 taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga tcgaacggtt
     5701 gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta tgatgacaat
     5761 cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa cttttgaaga
     5821 cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc agtacttcaa
     5881 aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata tccaggaagg
     5941 gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa tggggatttc
     6001 ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca gtctgttgct
     6061 cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa
     6121 tacattttgt acgctgacat gaatccagaa tgacttttat acaaaataag aggttgtgaa
     6181 aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct catatttttc
     6241 gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa
     6301 aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa
     6361 gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt gctcaaaaaa
     6421 cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat cagaacgctg
     6481 atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc aaacgaaggt
     6541 cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct ttttgttcat
     6601 catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct aatttgattg
     6661 catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc gtttcagggt
     6721 atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca tagtctgctg
     6781 tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca tgtgtatatt
     6841 gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg
     6901 tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca acttgtttag
     6961 ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct ttagcagatt
     7021 ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga gttgataatt
     7081 ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa
     7141 agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt tcattcccta
     7201 ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca gcagctaagt
     7261 cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga ttcaggtcat
     7321 aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta
     7381 aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga
     7441 aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca tcacggaacc
     7501 attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac caagcacgag
     7561 cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg tataaacgaa
     7621 gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt ttgtaagcga
     7681 cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg attggctgat
     7741 aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc
     7801 ccattgagat tggtttggca atattaggag aagacatgtc gattggtact gttccttgat
     7861 tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc aaaacttttt
     7921 tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact tcgatttttt
     7981 caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt ggcgcttttc
     8041 ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca gctgattttg
     8101 gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca gcgtcataga
     8161 cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg ttcccttgat
     8221 tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact agagacgaga
     8281 tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg atatcggtag
     8341 acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt cttcattcgg
     8401 cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt gttcaacaaa
     8461 tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt
     8521 tcataaaaaa acaatccaga tggcctaaag gagaagaaat accacctgga tagctataaa
     8581 tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg ttccttcttc
     8641 agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag cgtttggttt
     8701 tgcttcaaca aatgcaatag ctgcttctac tttcggaagc atgcttcctg gagcgaattg
     8761 cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt tcttttgatc
     8821 tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca gcaaatccgc
     8881 atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg cttctcgtcc
     8941 ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag agactgtgat
     9001 aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga tgggtttagg
     9061 ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca cagtggagtc
     9121 agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg gtttacttgg
     9181 atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag aaaccacatc
     9241 tttatcgatc cctttttcct tcagtacttc gcccatggcg ttttgtaacc agtaaccgat
     9301 agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt tctcagaatc
     9361 agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt gggagatgat
     9421 caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca ttaacgcttc
     9481 ttgttgggct tttgcacttg catccgtaga caagatggca ttgcccccta aagcgacaac
     9541 tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt ttctagataa
     9601 caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt cgtgagatga
     9661 atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg ccatgatcgc
     9721 tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt tgctgcggaa
     9781 gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt gttcaccgta
     9841 aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt
     9901 gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt tgatacgttc
     9961 ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga cgtctgctcc
    10021 gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt tttcagcaaa
    10081 gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg caacgatacg
    10141 catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat tgtttcgacc
    10201 atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt ggatagttaa
    10261 aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc aaactggaac
    10321 accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga attcgattcc
    10381 gtcaaacata cgtcctagta ctttagctgt atcttctgtg gattcttttt tacctaattg
    10441 gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg cagtagtaaa
    10501 tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt ttccttctag
    10561 atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa
    10621 atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc cttggaatac
    10681 tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt aaagaggaaa
    10741 tcccgacggc agctgacagt aagactgccg tcaggaaaca aattacttca agtcttcacg
    10801 gacaagtggt tggctcatgc agcgtggacc accacgacct cgtgacaatt cacttgaatt
    10861 gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat aatttcggtt
    10921 ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat tccattgttc
    10981 tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat catctaatcc
    11041 aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga tatcatcacc
    11101 atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg ctggatggat
    11161 agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt
    11221 gttcggaatt ttgattgcca acactttttc aaatcctgaa ttttttgcga ataggtggcg
    11281 tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag ctactacttt
    11341 atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac gatccaacca
    11401 tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt ctgtaaacat
    11461 cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat ttcccattga
    11521 tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa atggatagtc
    11581 atcgtctgct gacagatcat atagactgct agaacgtact tcgatatctt ttgtacgaac
    11641 cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga actcatgaag
    11701 accctctctt acagcattcg atgcaatatg agattcatcc aacattttat tcaagaattg
    11761 ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt aaagagtctc
    11821 gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag caatcggaag
    11881 atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat tttccacttc
    11941 ttgtccaggc cgtttcagta aaactgtctt taatttccct atttcagaga aaacgtgaat
    12001 aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga tatcctcctt
    12061 ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac attaatattc
    12121 gataattaaa taattaaacg tttttctatt tttaagcgta atttattcat tttttattct
    12181 gtatacactt tcaaaaaaat atttattcat tgaaaaaatt tttttgaata aattaaaa
//
LOCUS       DAZBTH010000078        11792 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000078 DAZBTH010000000
VERSION     DAZBTH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.080"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..341)
                     /locus_tag="ACHGNW_002033"
     CDS             complement(162..341)
                     /locus_tag="ACHGNW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926311.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(357..557)
                     /locus_tag="ACHGNW_002034"
     CDS             complement(357..557)
                     /locus_tag="ACHGNW_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926312.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(722..1429)
                     /locus_tag="ACHGNW_002035"
     CDS             complement(722..1429)
                     /locus_tag="ACHGNW_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926313.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1422..1859)
                     /locus_tag="ACHGNW_002036"
     CDS             complement(1422..1859)
                     /locus_tag="ACHGNW_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926314.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1874..2125)
                     /locus_tag="ACHGNW_002037"
     CDS             complement(1874..2125)
                     /locus_tag="ACHGNW_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926315.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2125..2331)
                     /locus_tag="ACHGNW_002038"
     CDS             complement(2125..2331)
                     /locus_tag="ACHGNW_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN6926316.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2768..3028)
                     /locus_tag="ACHGNW_002039"
     CDS             complement(2768..3028)
                     /locus_tag="ACHGNW_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926317.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3202..3306
                     /locus_tag="ACHGNW_002040"
     CDS             3202..3306
                     /locus_tag="ACHGNW_002040"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN6926318.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ACHGNW_002041"
     CDS             complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ACHGNW_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6926319.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3935..4834)
                     /locus_tag="ACHGNW_002042"
     CDS             complement(3935..4834)
                     /locus_tag="ACHGNW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN6926320.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4847..5440)
                     /locus_tag="ACHGNW_002043"
     CDS             complement(4847..5440)
                     /locus_tag="ACHGNW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN6926321.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5516..5665)
                     /locus_tag="ACHGNW_002044"
     CDS             complement(5516..5665)
                     /locus_tag="ACHGNW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926322.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5681..7885)
                     /locus_tag="ACHGNW_002045"
     CDS             complement(5681..7885)
                     /locus_tag="ACHGNW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN6926323.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7896..9356)
                     /locus_tag="ACHGNW_002046"
     CDS             complement(7896..9356)
                     /locus_tag="ACHGNW_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN6926324.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9417..11789)
                     /locus_tag="ACHGNW_002047"
     CDS             complement(9417..11789)
                     /locus_tag="ACHGNW_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN6926325.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2931 a         2464 c         1981 g         4416 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
      181 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
      241 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
      301 aacgtcacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
      361 cgaaataacc ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt
      421 aacacaaagc gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt
      481 aaatgtagag ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga
      541 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct
      601 tatttcgcac cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat
      661 tcccttaatt tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg
      721 gttattgacc ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
      781 tttggatttc ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt
      841 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
      901 cagtgagttc ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc
      961 tagtaaatac aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt
     1021 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     1081 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     1141 cattcatgat atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt
     1201 caggacgacg tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg
     1261 tttcactttc catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca
     1321 tacaactaaa gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta
     1381 aaaattgcga aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc
     1441 tccgattcaa tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag
     1501 tcgctagcca ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag
     1561 gcttgatatt cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag
     1621 gcttggaaaa accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc
     1681 gcaatgagat tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt
     1741 tccttgtaat gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt
     1801 ttttgtgcca ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata
     1861 ttttttctcc tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca
     1921 tgttttcttg ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag
     1981 tcggtgcatc ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt
     2041 ctgctacttt ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag
     2101 tcaataattt gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata
     2161 atcgccataa gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct
     2221 tttgatacat ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt
     2281 tgggaataat tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc
     2341 tgcctcctct taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg
     2401 gccagaatga atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt
     2461 gtccttgtcg ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag
     2521 agatagcgaa caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt
     2581 tgcaagacac catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg
     2641 tggcggttgg agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa
     2701 ggtcttttcc tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc
     2761 tcttctttta tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt
     2821 caatgactaa ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat
     2881 catccataat taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt
     2941 catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt
     3001 tatcaagcaa accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac
     3061 agctaaaatt gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag
     3121 tttgtgacgg tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat
     3181 cttacagaaa ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc
     3241 ggttctttga tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa
     3301 aaataaccgt cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc
     3361 accgtcttaa acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt
     3421 ataacaactt tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt
     3481 gattgtccgc caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca
     3541 tcccgttgtt caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg
     3601 ctttctgtcg ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca
     3661 tacacacgtt gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg
     3721 atcaaactgc cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca
     3781 cagttaataa aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct
     3841 agggtaaaag tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt
     3901 aagcgtccta ctaaagttac attattgatc atctttatct ctccatttct tgattcattt
     3961 gttgtgatct tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca
     4021 ttaggtgatc aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa
     4081 ctgagcgata tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt
     4141 ctctttcata ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa
     4201 cacttttgta taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt
     4261 agtaatcttt tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg
     4321 caataatttg ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt
     4381 ggtgatagtg tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa
     4441 acggtacacg tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat
     4501 cggcttctct tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt
     4561 ttctagttaa atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa
     4621 atgtcgtatc gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa
     4681 agccgccttt caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac
     4741 gatccatcat aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat
     4801 ttcttgtttt tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt
     4861 gcatgtggta tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac
     4921 ttggatctct caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt
     4981 caaattcata tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa
     5041 taggatttcc ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca
     5101 cgaaatgaaa ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg
     5161 gaaaagttaa gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg
     5221 tttcatgaga gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc
     5281 catgaccatc ctctacataa taaccacgat caactttgat caattttcct tgattcacca
     5341 atctttgtac agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc
     5401 catcgcgaat agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt
     5461 acaaatacac tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc
     5521 gatctaattc gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt
     5581 tatttgcacg taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt
     5641 gttgtttgtg tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg
     5701 ttcttttctt atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt
     5761 cttttaaaac gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt
     5821 ggcttgttcg gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg
     5881 caatcgttgt ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat
     5941 aaagcaagac ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct
     6001 gtttttcact ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt
     6061 gtcccggtac gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag
     6121 cttgtgttcc tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt
     6181 cggcacttgt tagcagcggc tctttttcca cggcttggca aagtaaaatc cctttatttg
     6241 tcaccacaag cttatttttt tctaccttga tatattcctt ttgtttcaaa gtttcaataa
     6301 tatttgctcg tgttgcttct gtaccgattc cttctacttc tttcaatatg gattgtgctt
     6361 cttcgctatc cacggttttt ccagctgttt tcatcgctgt gatcaacgta ccttcggtat
     6421 aaggttgtgg tggcttcgtt tctttttcaa ggacttgtag atctaccgtc accgcttctc
     6481 cttttgtcac tttaggaagt agcggttctt cttccttttc ttttctgtcg ctctctttgc
     6541 caaacagctt tttccaacct agatctactg gtacttttcc gactgtcttt agctgaagct
     6601 gctgaacttg cgtcaagatc gttgtttctt cataggtata cttttccgca aacatggcca
     6661 ccgttgtttt taacacttgt tcataaatag cctgctgcag tggacttaat tgttcaaaag
     6721 ccgctgccgt catgaccgtc tttgtaggaa tgatcgcgta atgttcctgg actttcttat
     6781 tattcacata tcgactattc ggtgtatgaa taggagtagg aatggcttcg gcctttaaaa
     6841 agtgtttgta ttcgtctaag tgatccaaca gatacgcata ctccccttct gtaatataag
     6901 gggtatctgt tcgtgggtag cttagatatt tcccttcata caacccttgc atggcttcca
     6961 aagtatcttt ggcactagct ttcatgagct gattcatttt agattgaagg ctggatagag
     7021 aaaaaagacg tggacttgcc gcactttttt tctcggtttc aacttggtgg atcgtacctg
     7081 gctgttttcc caattgaacc cccttttctt ttaaataatc ttcaaatgct tcttgcgtcg
     7141 cttgggtttc attcggatca agcttcgcat caaatgcgcc attttgagcg attacttggg
     7201 cttttccttc aaaatatggt tctttttgga agtttctaat cttttcctgt aattgataaa
     7261 tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccttttt
     7321 gctgcaagag taaactatac agcgggctac cgttcatgcc gatcaaccaa tcggcgatct
     7381 ggcgtgcttg tgcttcttca aattttgggt aggtctcttc tgcatctcgt aactgctgga
     7441 atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta aatatctttc
     7501 catgatcggc gcctgcttgt tgaatgatcg tccaagctat cagttcccct tcacgatcgc
     7561 tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc accttgaatt
     7621 gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct
     7681 ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat
     7741 gaccaaaacc atacgtgatc acagtttctc ctgaaaacaa tcgatcttgg atctcaaaat
     7801 acccatcttt tcgggtagct ttagaaaagc tatcggcata agccttcgct tgtgacggtt
     7861 tttctgctaa aataacggtt ttcatcatga cgatttcact ccctttcttg ctgtctacct
     7921 tgttgaaaca acatggcacg tttttgcttt ctgatctctt gttggtagat ttcatgcttc
     7981 aattgatcat tccaatcctt ttttacttca ggtacaaatt catgttcatg atttttatag
     8041 cctttgtagt attgttctgc tttataagag aaaagaatct gagcattaat atcccctttt
     8101 tcttgataaa aacgatcaaa atcgtacgta ccatccacgc gttcacaagc ttttaattct
     8161 tttgcgactt ctctacaaat atctttcaaa ttaatagctt ctattgaatc gttaacagaa
     8221 agctgctgac caaaagacgc atttactttg tgagcggaaa ccaattggtt cgttaattgc
     8281 agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgatctt ttcttctttt
     8341 gcgacttctt cataaatgac tttgtattct tttggcactt gccaatcatt agcgattcct
     8401 cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt
     8461 tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag
     8521 gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt
     8581 tttaaccctt ccatcgaaac taaccacgta tcgtttaact gatcacgatt tagtgcagca
     8641 tagctaagta aatcaatact actctcaaaa aattttaact gcttagggtc accgatcttt
     8701 aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca
     8761 ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt ctcatttgtc
     8821 tgtggatctt tccataaaaa ggcgaccgca tgatctaaac gatcgtctcg aactaagaaa
     8881 ttttgccgtt catgttttgg ctttagttcg gcaatcaacc cttctttttc tgcataatca
     8941 atcagccact ctggtagttt tcgttcttcc ttcagataat tccttccttt ttctgtgagt
     9001 ggttgataat caacggggtg atctttgtac ttttctgtct caaaatgaag attttccact
     9061 cgttctgttt tgtcttcatt ctttagaata aaatcagtgg ctgctttaaa ttgttgctta
     9121 gggtcttgga tctctttgtc tatgaaaaaa agtttagcta attcaataat gtcgccacta
     9181 atattttgac tgttccaata aaaacgttgt tttcttcgat caaaaacaaa tgaatcatgg
     9241 tcttctaagc gatagtctcg tcctttattg actacagcca ttccattgtt tcgagcaaaa
     9301 tccactatat cgatctgttt acatcgatca ataagactca tttttttctc ggtcatttta
     9361 aaacctccca aaagaaaaaa gactatccgt ttttcagata gtctttttta cttgttttat
     9421 acacgttctt tttgtgacaa ttgttgttca taatagattt cattttttcg ttttgccact
     9481 tctgacactg tctcaagtga aaatcggaat gaatttccta gcgcaaattc ttctgaaggt
     9541 aacgccgttt ggatctcttt tccataactt tttcctttta aaattacttg attatgctgt
     9601 ggatcttcaa taaacgccgc atccttcaaa tgtaacgttc gacccgtatc ttcttccatt
     9661 actttcccgt ctttcttgtc gatccaaagt gaaccaacag gaacaatatt cttttgttct
     9721 gccgtttcta cagtattttt atgctcctgc ttcacttctg ctggttggat ctctggttct
     9781 ctttctctgt attgattgat ttcattagaa atggtttgaa tgaattcatt tgcgacacgg
     9841 ctgacatcat tcatgatttt cccacgttcc gcagcttcct tatcatccaa cgtctgacca
     9901 ttcttcgtcc atgctgcaat ataaggaata gctttctccg aagtgtccat tccaaaatgt
     9961 ttgcacacaa tataagaagt catttctgcc tgcaattctt tagttgcggt atctaattct
    10021 gaaaatttgt gcattcttgc atgggctaat tcatgaatac ttgtagctaa atgctgcgta
    10081 ggagtatttc gagtattcat gacgatttct tcttcgccag taaattcgtt acgaacatat
    10141 gccccccgtg cttgtcctaa ttctctttga tagacttcac tttctgtcat atctcgaatt
    10201 tgtattccaa tattgttggc taccgcttga atcccagctt gaagttctga ctgtcctttt
    10261 ttatccaatt gaaaatcaaa gacacggttc ggaaagattt tcggatagtc ttcaggtttt
    10321 aactgtgttt gtgtgatatc aaacactttt tcaatcgtgt agtaaggttt tttgatcact
    10381 tcgatttgac cactttcgat tcgtttttta tcttctggtg tagcttctgt ttctcgaaca
    10441 aagccacgtc ctttctcata aaatcctttg accgtttttc tgacaaagat cttgttccct
    10501 tgttcccctt ttttgatata gattccttct tttttcaacg cagcacgacc taaacaagcc
    10561 gttgcacctg gaaattgttc ctcaatcaat aattgatttc taaaggatcg ttctggaaaa
    10621 cgagacataa aattcaaaag ttctttcacg tcttcaggag aatgggtatg ctttttcacc
    10681 gcatccagtg cctgttgcga aagggatttg acttcttgct taatctcttc tgccgttcgt
    10741 ttttgatagc ccttttgttt tgtttttgtt tctttcgact gcatttgttc tttcgaaaca
    10801 ggttcatttt tgaccgtttg tttttgttcc ttttcaggta cttgttcgca caattttgct
    10861 aactctaaat agatgggttg attactgttg acgccatccc ctagatccat tcggatctga
    10921 tcgactactt ctccgttctt cacttcttca aaatagaatt tataataacc tgcttctgcg
    10981 gcgtattggt tatccaacgt tttgagttga tcgatcaatt ctaatgttac gatctcccca
    11041 ccataacttt tcaatccttt ttctggatca ttttcgttga attctactcg atagaaatga
    11101 tcgttttctt tctccttcaa agaatcagtt ggcgcagcct cttcaagtaa ttcacgaaca
    11161 gctgcttttt cctcttccga cgtggcttgg tatttttttg attcctcaaa tcccccgttt
    11221 tcattcagtt ctaaagcaac ttttcgatag tctttcaaaa aataaatggt tggatttttt
    11281 ttccattgcg tcactcgtcc gctgtcaata attgcttgta cttcaggaag aagattttct
    11341 tttccttttt ctgatctttc tttcagctgt tctaatgttt ccaatttccg ttggtatttt
    11401 cggatcgtag cggctgaaaa agtcgattca cctttttcaa agcgttcgat ttcttctttg
    11461 attcttggaa tattttcgat tgtcatatcc aggccgccgt acttgttata gcccatttcc
    11521 tttagctctt cttgtctttc aagtttttct acctgttttt cttgttgctt gatctcttct
    11581 ctcagactat gcactttgtt ttctaaacgt tcttgttgcc taaaccaaga atgcccattt
    11641 ctcttatcgt tcattggctg accgttggtc tttctctggt gttcaaacat cttctcaatt
    11701 tgttcgttta attcattttg caatcgtact aattttcttt tggattttcc caagcgacta
    11761 ttgtctagtg gttctttttc ttctgccatg tc
//
LOCUS       DAZBTH010000079        11763 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000079 DAZBTH010000000
VERSION     DAZBTH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.081"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            347..616
                     /gene="rpsN"
                     /locus_tag="ACHGNW_002048"
     CDS             347..616
                     /gene="rpsN"
                     /locus_tag="ACHGNW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6926326.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            631..810
                     /gene="rpmF"
                     /locus_tag="ACHGNW_002049"
     CDS             631..810
                     /gene="rpmF"
                     /locus_tag="ACHGNW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6926327.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            842..1219
                     /locus_tag="ACHGNW_002050"
     CDS             842..1219
                     /locus_tag="ACHGNW_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926328.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1240..1389
                     /gene="rpmG"
                     /locus_tag="ACHGNW_002051"
     CDS             1240..1389
                     /gene="rpmG"
                     /locus_tag="ACHGNW_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6926329.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1413..2396
                     /locus_tag="ACHGNW_002052"
     CDS             1413..2396
                     /locus_tag="ACHGNW_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6926330.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2426..2548
                     /locus_tag="ACHGNW_002053"
     CDS             2426..2548
                     /locus_tag="ACHGNW_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6926331.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2566..4089
                     /locus_tag="ACHGNW_002054"
     CDS             2566..4089
                     /locus_tag="ACHGNW_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN6926332.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4113..4352
                     /locus_tag="ACHGNW_002055"
     CDS             4113..4352
                     /locus_tag="ACHGNW_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926333.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4545..5006)
                     /locus_tag="ACHGNW_002056"
     CDS             complement(4545..5006)
                     /locus_tag="ACHGNW_002056"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6926334.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5201..5419)
                     /locus_tag="ACHGNW_002057"
     CDS             complement(5201..5419)
                     /locus_tag="ACHGNW_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926335.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5537..7153
                     /locus_tag="ACHGNW_002058"
     CDS             5537..7153
                     /locus_tag="ACHGNW_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6926336.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7394..8734)
                     /locus_tag="ACHGNW_002059"
                     /pseudo
     CDS             complement(7394..8734)
                     /locus_tag="ACHGNW_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9172..9318)
                     /locus_tag="ACHGNW_002060"
     CDS             complement(9172..9318)
                     /locus_tag="ACHGNW_002060"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926337.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9357..9551)
                     /locus_tag="ACHGNW_002061"
     CDS             complement(9357..9551)
                     /locus_tag="ACHGNW_002061"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6926338.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9461..9793)
                     /locus_tag="ACHGNW_002062"
     CDS             complement(9461..9793)
                     /locus_tag="ACHGNW_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6926339.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10307..10498)
                     /locus_tag="ACHGNW_002063"
     CDS             complement(10307..10498)
                     /locus_tag="ACHGNW_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926340.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10592..10978)
                     /locus_tag="ACHGNW_002064"
     CDS             complement(10592..10978)
                     /locus_tag="ACHGNW_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6926341.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3923 a         1830 c         2253 g         3757 t
ORIGIN      
        1 ttggcgataa accagttaaa cataaaagat tgaataaaaa gttatagttt ctttcatatt
       61 gccgttttaa gtagaggaaa agctacttaa aacggtattt gtgtttgatt ggactaataa
      121 aagtgaattc ttatttttca gagcctaaac tgcatattct tttaaatgat ttgattatct
      181 ctatgtaccg ataaattcgt tttcaaatag ttatctattc atatgagttt tatttcatgt
      241 tatttattgt tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat
      301 tacgatttga ggtgtctaaa tcaaaataaa aggagtataa aggtatatgg caaaaaagtc
      361 gaaaattgct aaagcaaaga aacaaatggc gatgattgaa aagtatgcag ataaacgtca
      421 ggaattaaaa gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc
      481 gaatcgtctg aggcttcgtg atcagacgga tggtcgtcca agagggtata tgcgaaagtt
      541 cggtatgtcc cgtattaagt ttagagaact tgcccatcaa gggttgattc ctggagtaaa
      601 aaaagcaagt tggtaaaagg aggaaatatc atggcagtac cagcaagaaa aacatctaag
      661 gcgaagaaac ggttaagaag aactcatcaa actgtagtaa aaccagaaat ttcatttgac
      721 gaagcgaatg gtgattatag acgtagccat catgtatctt taaaagggta ctataatgga
      781 aagaaagtaa ttaaaggagc ttcaaaatag tttaatatat aacttttagg agggaatcaa
      841 aatggaaatt atttacccac ctttagttga agaaggatta aagtattatc ttgaaacgac
      901 ccagcaatcg ctagataaat gcacttttta tcgttcgatg gtagagagag gaattattac
      961 tgaaacgggt ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag attactatga
     1021 ggaccaaggc ttatcatttg atgatttttt aagaatctat ccaatttttg aagagtatga
     1081 cgaggagtta tttcaatgta tcgatggtta ttgggagata cctgttgata tgaaagaaaa
     1141 tttagtttca caattggaat ctggcgagtt aacttttgaa gatgcacaac aaattcaagc
     1201 atatcttgaa gatcgataag tattgaagga ggaagattta tgcgtcaaaa tattattttg
     1261 gaatgtgtcg aaactggaga acgtctgtat ctcaccagca aaaataaacg gaataaccct
     1321 gaacggttag aattaaaaaa gtattcgcca aaattgcgca gacgggcaat ttttaaagaa
     1381 gtgaaatagc taagtggtaa aaaggtgaca aaatgggcat accagttaca atcgtttctg
     1441 ggtttttagg ggctggaaaa acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc
     1501 ccagggaaga aattattata attgaaaatg agtttggtga agttgggatt gatcacaaat
     1561 tattagttca tagcgaggaa cgggtgttac aactaaataa tggatgtatg tgttgcagtc
     1621 ttcgcagtga tttattagca atattgattt ctttactgga aacaatagaa gaacatcaac
     1681 aaccaatttc acaaatcatc attgaaacta caggtatagc agatccccaa ccaatcattc
     1741 aaacactttt aacagcgcct caactaagag gaagagtata tatcgatagt ttattgactg
     1801 ttattgatag tgacagtttt gaacttattg aaagagaacc acaagccctt aagcaacttg
     1861 tcatggcaga tcgcattttt gtgtctagaa aacaaaactt gaatttatct gcactaaaaa
     1921 aattacagag agaaatcaaa agtattaacc ctttatccga tattcgaatg ttcacacata
     1981 gtgaaacgat tcaaaaaaag gatttttata atttgaaaaa atttaatcat cccttagttt
     2041 ttgaaagtga ttcatcagaa aatcactata atggcaaata ctatcatcaa caccatcaag
     2101 tacatgagtt taaatcaatt ctcttaactt cagatcaaga cataaaaaaa gattttttac
     2161 tccaatggat tgactggctg attttaacca accaagggag cctgtatcgt tttaaaggat
     2221 tgattgcttt acaagagcag gattttatag ttgccatgca agaggtaaac gaacaggtta
     2281 attttcaata caccactcga caaaaagacc aaaccactat tattctaatc ggaaaaatgt
     2341 tggatgaaga aaggatcaaa cagagttttg ataaattact ggcggaaagc aaataatgat
     2401 aagtagtgat tgggggtaat ttctaatgga gaaaacggat atatctagtg catatcgacg
     2461 acttaaaagc ccgaatatta aaactagaaa aagggcatta aaaattatca aagaacataa
     2521 acgaaataaa atgaagaaat tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt
     2581 gtaggactta ttgggatagc agctctagga ttattgggag cttgctccag cactaatgat
     2641 gcaaaagtat cgaatgataa agatggaaaa ttagaaattg taacaacatt ctatccaatg
     2701 tatgatttca ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat ggttccagcg
     2761 ggatcagaac ctcatgacta tgagccctca gctaaggata tggcaaaagc tcatgatgct
     2821 gatgtttttg tatatcataa cgaaaacatg gaatcatggg taccaaaagc taaagaaagt
     2881 tggaagaaag ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt attgccaggt
     2941 agtgaagaag aagatcatga tcacggagaa gaagatcacc atcatgaact agatcctcat
     3001 acgtgggttt cacccaaaat ggcaattaag gaagtttcaa atatcaagga tcagttagtt
     3061 aaattgtatc cgaaaaaagc aaaagtattt gagacaaatg ctgaaaagta tttgactaaa
     3121 ctgaaacggt tggatgctga ctataccact agtcttaaag aagcgaaaca aaaaagtttt
     3181 gtgacgcaac acgcagcctt tggatattta gctttggatt atggattgaa acaagttcct
     3241 attgctggac taagtcctga agaagagcca tcttctggtc gattagctga attaaaagag
     3301 tatgttaaaa aaaataagat caattatatt tactttgaaa agaatgctaa tgataaaatt
     3361 gcaaaaactt tggccaacga agctgggata aaattggaag tactgaatcc tcttgaaagt
     3421 cttacaaaag aacaaatgga taatggtgaa gattatgttt cagtaatgga agacaatcta
     3481 aaagcgttag aaaaaacaac tatggttgct ggtgaagagg tagttccaga aaaagaagcg
     3541 aaggatgaaa agacagttgc taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa
     3601 ttgagtgatt atacaggcga atggcaatct gtttatccgt tgttaaaaga tggaattttg
     3661 gatgaagttt tcgattacaa agcaaaaatc aataaagaca tgacagctgc cgagtacaaa
     3721 gattattata ctactggcta taagacagat attgatacta tcaacattaa agacaacacc
     3781 attgattttg tagtcaacgg tgaacatcat caatacacat acaaatatgt tggatacaaa
     3841 attttgaatt atgaaaaagg aaaccgtggt gttcgtttta attttgaaac tgatgatgca
     3901 ggtgctggtc gttttaaata tattcaattt agtgatcatg gaattgcacc aagtaaggca
     3961 gagcatttcc atattttctt tggtggagaa agtcaagaaa aactctataa tgaattgcat
     4021 aattggccta ctttctatcc agcttcattg tctaaacatg aaattgctca agaaatgatg
     4081 gctcattaaa attcaatttt tggaggaaat acatggtaag aaaagtatta ttgtctatgg
     4141 attccaagaa tattgatgct tatcggcgtt atcgatttgc aaagcaaatg agattaaatg
     4201 ggagtacatt agctgatatt gctgcaactt tacatgttag tattgacaca gttcatagag
     4261 tattaaaacg ggatcctgat tgttgtttta tcaaggtcaa tactggaaga agtagacgtt
     4321 tagcaaaacg gcataatagc ttggccaatt aattgataat accagagtag atcatttttc
     4381 ccttatttgg atttgatctg ctttttatta tgtcttgaat tgtcaaatca agcgcaacac
     4441 tttatcaaag aagacttcat aaggtgttct ccaatctaaa cactttctag gtctgttatt
     4501 cagtttttca atcatctgat ttatcgttga gttatctacc gtagctatct ctttcgattt
     4561 tggcaagtat tctcgaatga gtctattcgt attttaattt gttcattgtt gccaagggca
     4621 tgaggatctg gaaaataaaa gggcatatta ttcaatgctt ctgtaactgc gaaatgattg
     4681 gagaattctt ttcctctatc tggagtgatc gttttgaccg cctcatttgg aatactgctt
     4741 aaaagttcaa tcattttctc gctcacataa gcggctttct ttctggggac cttctttcct
     4801 gctaaaagga agcatgattt tctgtctgtc aaagtcacta aacaagtacc accagttttg
     4861 ccagctacaa tatcggcttt ccaatgacca aaatcacttc tatcatctgc ggattttgga
     4921 cggccatgga tcgaatgagt aaggcaaatc tttccacgag actcgacatg attttttgta
     4981 tgtcttgttt ttcctcgatg gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg
     5041 ggttctgtat cgaaaagatc attgcaaatt gcacgttaga tcgtgataaa actaattgaa
     5101 aagtccatat gttcatgctt caaacgattc gagatttcct ctggcgacca ctgaaaatcg
     5161 acaaataaat gacgaacaat tgaccaaact cacggatcat ttagaatgtg ataacgtccg
     5221 catttttttc ttcgataagc ataatttttt tccgctttgg aaggtgaata gtttttttct
     5281 tgtttcttct cagttcatgt aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta
     5341 tactaataga aaatccttct tcgtgaagca gaaagattct ttctcgttcg agtatagtaa
     5401 gatgacgata ctggctcata attattttcc ttctaggagt gttctcgaca aacttatact
     5461 agttggatat cgattatgac tcatttttat gtgttgcact taaagtgtaa attcaaggta
     5521 tagaaagagg ttccctatga aaaaaatacc tattacaatc ttaactggct atcttggttc
     5581 aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt
     5641 cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt
     5701 taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg
     5761 cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt
     5821 tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa
     5881 aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt
     5941 ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc
     6001 tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga
     6061 gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa
     6121 aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg
     6181 tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta
     6241 ttcagtgggc tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga
     6301 agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt
     6361 tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg
     6421 gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagct ataaaagtat
     6481 tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca
     6541 aaaaaatccc gaattgaagg aagcctggca tccgttgttt ggtgaacgca ttaataaact
     6601 agtttttatt ggtatgaaga tggatcaaca atgtattaag gaacaacttg agggttgttt
     6661 aactgattct cttaatgcag ataatttccg aaaaatttca ccatttccat ggaaaaagtc
     6721 taatatgttt agccagatat cgttaatgaa atttttaaga aaagctggtg tcaagatagg
     6781 cgatgtcata attattggca ttttgattat tacctctttt attcccttat ttgtattctc
     6841 tatagaaaag gagaatgaaa caactgaaga tgctatcaaa tatgccatcg ttcgaattga
     6901 tggcaaggaa atggatcgct tcgatttgga taagatcaat cataaactcg tgaattatca
     6961 tcctgcgaag ggacaatata atattgttga gattaaagat ggaagaattc gagttaaaga
     7021 agataatagt cctgatcaaa tcgcggttag aactggatgg atctcaaaac ccggtcaaac
     7081 gagtatttgt ttaccccata agttagttat tagtattgag aaaaaagaaa gcacagatta
     7141 ctacatttac taatctggaa atagatgagg aggtttttaa taagaaaaag atattattat
     7201 taataatagc aggggcttta ttaagtgtgt taagcgcttg tggacaagaa aaagaagcgg
     7261 tgacaacagg cacaagtatc tttaacggga cctgcaatgg tttagttaac agtcggttag
     7321 attacttatt ttggctcttc gtcaatgagt gttggtgaag aaattcaata gaataatcga
     7381 gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttctg gctttagtca
     7441 gttgggtagt attcttagtt ttcttaaaat attttcctcg atataaaaag attcgttctc
     7501 ggtagttttg aaagtttcta tagccatagg caattcgttt tagtactttg atatggttat
     7561 tcaaacattc tatagggccg ttggaatagg gaacgcgcaa cgctcgtttg atttctgttt
     7621 ggtatttctt gaaggtcgtg aacactggtt ggtagtgctc tggcaactcc ttgtagtctt
     7681 cttttaggag cgcgacgaat tcaggaacat cttgtcttct gataacggaa agaaaatcct
     7741 ggtagacttc gtagcctcgc tttaaggaat catcataaga taatagccga tcgactatct
     7801 ctgcttctgt cagatgatct cgaaatgacg gacgccagta gcgatgctga cctttcaatt
     7861 cccacgcatt cttttgaagt agtttccagt atttctttaa gtgcttcgac cgacgatccc
     7921 cttttttaaa ggtcttcatt acttgaattc ggtggttggt aaaagcccga ccaatgtgtt
     7981 ggctaatatg aaagcgatcg gcgacgattc gggcattcgg aaaacactct ttcaccaatg
     8041 tgcgataggg tgtatagaaa tctgaaacca ctagacgcac ttgttcacgg acttttatag
     8101 ggtagcgcaa gaaatagtcg cgtaaaggat tgagttgccg attttccaca atatcaatca
     8161 gttgatgggt cttgccgtcc atcatgacaa agctcatggc tcccgagaca tttttcactg
     8221 atttgaattc atcgaaacac accacttctg gtaacagctg gctcaaagaa atggttttag
     8281 gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc atacgtgcga
     8341 tcaaagacat agaaagaggt tcgcttaata gctcaagaat ggcttgtttg actctgcgag
     8401 caatggagca gcgacgatca ctaactgaat cttctgctaa aaaagtcttt ccacagtgtt
     8461 tacatttgaa gcgctgtttg ttaatccgca gaatggtctt gtagttactg acatcattca
     8521 ataagatgga tacttttttc cagccctatt taatgatcgt ttgttcattt tgatagtggc
     8581 gatgaaagca ttcctccggt tgataggtca atgtgccatc aaaaacaaaa catgtttcgt
     8641 cattgattcg ctctttgcga aaggcatctt catgaaagat aagatttagg tctagaatat
     8701 ctagtaactc tttggtaaac tctgaatagg acatcttgaa acactccttt gaatggtttt
     8761 cgtcgacttt attctaaagg attttaaggt gtctgtttgc gtttattaga aaaaagaaaa
     8821 agtattggca acagaaggcg tcaccaacac tagatattat agatccctta ttttcttcgc
     8881 ttcaaattgt caaggagtca tcataaattt tgtatggatt cttttgtttg ggtagtagtc
     8941 ttctaaataa tcttggatta acaccttcat ctctatttta cttttatatt tgttggagtc
     9001 gattaattct catttgattg atcgatgaaa cgcttcaatt actgcattgt caacaggagt
     9061 cccagtttgg ctcactgaat gtgtgatgtg gtggtcgttc aacagctctt gataaataaa
     9121 agaccgatgc tggctacctc tatcggaatg aattaccgta ggcaattctc cttaataaat
     9181 ttgtaatgga cggacgacaa tctctttttt catgtgtgaa tctaccatat agtaacgtag
     9241 aatacgagtt gctaagtcaa tataggtgga caaatagagg cgaccgtcat tacatggaat
     9301 gtaagtaata tccatcaccc aaacagcatc aatttggttc tgttcaaatt atcgattcaa
     9361 cattttttta ccatttcttt gattgttttt tctctaccat gagaagattt gcgtcgatga
     9421 aatcctcttg tgcataaatt catttcacgc ataagaacag ctacaacacg tttattgatt
     9481 tcgattcctt catcgtaaag tttttgagtg atccctggag atccgtatcg atctttgtgt
     9541 tcgtagaaca ctcgtcttcc tcttttagtc gtttattttc ctgttcaaga atgtagaaat
     9601 ctgctttatc aggtaatctt tttccttgtc cagcaaaagc atttttcccg tatcgacgat
     9661 attctgaaac ccatcgggaa attgttggac gagagatatt gtattcttca gcaattttct
     9721 tggtagttac ttttttctca aggatatcct gtgaaatctt tagtttaaat ttacgatcat
     9781 atgtttttct caatagagtt cattccttcc ggtaacgtaa ctgatagtca agaaaagtgt
     9841 atcatagccc aacttagtat cagggtttgt caactaaact gtggaagttg atagataaga
     9901 gtttttaaca accaaaattc tcttgactat ttttgaaatc agacaaattt tgattggaca
     9961 catagttgaa taaatctacc gtcgttacgg aaggtaaatc gcatgctttt caattctaga
    10021 ggagaagtcc tcgttgaagt accatagccc agttctggat tcgacctcct gcccacttca
    10081 tacgtaaata aagtaatttt aggagtgaga ctgtgacaaa agttattttt aagctaaaaa
    10141 cgaattaaaa aacgttgata attaaaatca ttcgttttag attctatgaa tcattataga
    10201 acgctgtaaa atcgatactt tataattata aagaagatag tttcaaattt attgttcgaa
    10261 atttgaaact atctttttac ttatgtcaca gactccctta gttgagttat tctttaattt
    10321 cgaatttcga gatagctaga tatattgcaa gcatacattc ttgataacaa tcttgatcat
    10381 attttccagt tctccaggat tgcttatgaa tcaaagagct aaatcttttg agaattgcct
    10441 caacagcttc ttcatttcca gcctttgcta aaatgaaatc ttcttttaaa gaattcataa
    10501 atctaccttc ttcccatgga cttataatct atttctttgg gaaggaggag aacgagcaaa
    10561 gaagcataca aaattagtta ttatgcagat attaaaccag cattttttga atttttttgt
    10621 aaagtcgatg ttttaaattt gtcgcaccct gtcttgtaat ccccagaacc tttccaattt
    10681 ctttatcagt tttatcaaag ataaattttt cgataagaaa aatttgttca ggttctttca
    10741 acaaaggaag gatttttgct aattttatat cttcaacaag gagagtcaca tttaaaacat
    10801 taatcggttg tttaaatttt atagtatctt tagtaaacaa atatggtggg agttgatcaa
    10861 gttgttcatg tttttgaatt tcttcttgtt ttttataata agtattagcg gtgttcaaaa
    10921 tgaccttttc aaagaatcga atcaactggg catttacgcg ttcgtcgtta tctgacatat
    10981 tgcacacctc ctccattaag atttaaaggg aaaagaggca aaaaacacga aaagaagcta
    11041 atgcaattat gctgcgctgc gaataacttc tataagctca catgcattga taggactgct
    11101 gatgtttatt ttcaaaaatg tttttttagt tgataatgga tagttatctt tatacttaga
    11161 aaagaaaatt catatatctt cttaaatatc aaacatgtcc ttatggctaa gaaaatgtct
    11221 tgagcttttt acgtataata aatcttttct aaaacctcct tttggataat attcttattc
    11281 gtgtatagat tgtatttttt atttcttccc ccttcataat taacaactag aaaagagttc
    11341 gaatagacac gtatttgaac gaaaattttt attggattcc caaattggaa tatttagaaa
    11401 cgaaaaattc ctattatctg tatagtgctt agataataac ggatcataat aattattgtc
    11461 tttgaactag atatatgtaa atagtattca tcatgatgaa aaatgatgag ttctcggtga
    11521 taaaatgtac tttttacggt ggaatataca tgaaagcgat aagtggatat tttataatga
    11581 agtaagttag cttattttga cagaggaata gtattttaga taacgtataa aatactattt
    11641 aatgatgtag aaaggaaaga gagaaacaaa agtgttttca gataaagaag tcttattaaa
    11701 gtctcttcat gtttgtttaa agttacctat cgtggttttt aattccgaat tcagtattat
    11761 aaa
//
LOCUS       DAZBTH010000080        11569 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000080 DAZBTH010000000
VERSION     DAZBTH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.082"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..871
                     /locus_tag="ACHGNW_002065"
     CDS             164..871
                     /locus_tag="ACHGNW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926342.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(1033..2205)
                     /locus_tag="ACHGNW_002066"
     CDS             complement(1033..2205)
                     /locus_tag="ACHGNW_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6926343.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2411..3226)
                     /locus_tag="ACHGNW_002067"
     CDS             complement(2411..3226)
                     /locus_tag="ACHGNW_002067"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6926344.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3376..3822)
                     /locus_tag="ACHGNW_002068"
     CDS             complement(3376..3822)
                     /locus_tag="ACHGNW_002068"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN6926345.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3819..4334)
                     /locus_tag="ACHGNW_002069"
     CDS             complement(3819..4334)
                     /locus_tag="ACHGNW_002069"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFN6926346.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4407..5321)
                     /locus_tag="ACHGNW_002070"
                     /pseudo
     CDS             complement(4407..5321)
                     /locus_tag="ACHGNW_002070"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5318..7645)
                     /locus_tag="ACHGNW_002071"
     CDS             complement(5318..7645)
                     /locus_tag="ACHGNW_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFN6926347.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8254..8400
                     /locus_tag="ACHGNW_002072"
                     /pseudo
     CDS             8254..8400
                     /locus_tag="ACHGNW_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8488..8781)
                     /locus_tag="ACHGNW_002073"
     CDS             complement(8488..8781)
                     /locus_tag="ACHGNW_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN6926348.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8818..9003
                     /locus_tag="ACHGNW_002074"
     CDS             8818..9003
                     /locus_tag="ACHGNW_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926349.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9184..10362)
                     /locus_tag="ACHGNW_002075"
     CDS             complement(9184..10362)
                     /locus_tag="ACHGNW_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926350.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10466..10780)
                     /locus_tag="ACHGNW_002076"
     CDS             complement(10466..10780)
                     /locus_tag="ACHGNW_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926351.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10887..11105
                     /locus_tag="ACHGNW_002077"
     CDS             10887..11105
                     /locus_tag="ACHGNW_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926352.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11025..11495)
                     /locus_tag="ACHGNW_002078"
     CDS             complement(11025..11495)
                     /locus_tag="ACHGNW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN6926353.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT         3351 a         2263 c         2018 g         3937 t
ORIGIN      
        1 atacttaata acagaactat agcaaaaaat cccgtgtatc aacacgggat tagattaagt
       61 ttcctcaatc actattcata acacaattat tataacaagt aagtaaacaa ttgaaaagga
      121 catttattga aaacggttgt tttttactta aggaggaaat ttagtgaaaa aattagttgc
      181 gttgtttttg gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt ctactactca
      241 aagcacaagt tcagctcagg cttccagcag cagtaccacg atctccgaaa gtacggcaga
      301 ttcatcgaaa gaacaaattg aaaagaaaaa agaagaagag aaacaaaaag cggaaaaagc
      361 tgcactagaa aaaaaagtga cagaagcgga atctgctttg tcacaagctg aagcaaatcc
      421 tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg aaaacaaaca
      481 agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag aagcagaaga
      541 agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc aacaagaagc
      601 aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc aacaagcaga
      661 acaggcgcga cagcaagcag cagctgaaca ggcttctcag caggcacaag ccgcagctcc
      721 tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat accatacaca
      781 taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag ctcgtggttt
      841 aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa taataaaatt
      901 cttctgaaac ataaaaaaat agaagctggc gttccagata gtaaaccaaa aatagcagtt
      961 tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct ctgctttttt
     1021 ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt ttcgcagttt
     1081 atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt ttacatggat
     1141 tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat cagaaaatct
     1201 cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag taatatagtg
     1261 tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc cgtttgtgtt
     1321 ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat ctaaataagc
     1381 gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc aaataaaaaa
     1441 tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt ttgcttgata
     1501 aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag gagtgcaggt
     1561 aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt cgtcaggtaa
     1621 gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct ggatgaaaaa
     1681 ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg ggagtccacg
     1741 atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac ttccttccaa
     1801 taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag gtaagctgac
     1861 attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg cattactagg
     1921 taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc ctggtgatac
     1981 gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt tttttatttc
     2041 ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct gggcaagaaa
     2101 atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa cgactaaaat
     2161 gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt ccacctattt
     2221 tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat tagcgaagca
     2281 aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag agaccgacca
     2341 cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta tgatttttgt
     2401 ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc actgctcctc
     2461 caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca gttgaaggca
     2521 agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga tcatcatcta
     2581 ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct ggtgcttttg
     2641 tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc ccttctgaat
     2701 cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac caagcatatg
     2761 ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt ggatcttttg
     2821 ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca tcttgtttga
     2881 tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta tccgtagttg
     2941 tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct gcgccatgga
     3001 tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt tctttttgtg
     3061 gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa ttgccgtcta
     3121 ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac gctgactcag
     3181 caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat tgattctctg
     3241 aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag ttcccagctt
     3301 tctggatttt gatattgctt tctttgatga catttttagc atataggtga atcgtgtcta
     3361 aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt cgacaccggg
     3421 ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac ctggaagagt
     3481 caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag cgtctgctgg
     3541 aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt tgaattcaac
     3601 gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta tttgattttc
     3661 tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg gtgtaggtga
     3721 tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca gcatgatagc
     3781 agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc cctcctattg
     3841 tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac tagcaataac
     3901 cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct tcctccattt
     3961 ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt attcgtcgga
     4021 tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc ataactgcct
     4081 ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg cggtttaatc
     4141 tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc atctttactg
     4201 gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat cccgttttct
     4261 tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag tttttgtttc
     4321 gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag atgtgtttct
     4381 actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt ttcactgttt
     4441 tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga actaagatct
     4501 tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa tgtatgaatc
     4561 ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg aggatcagct
     4621 gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt tacggtgatg
     4681 agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac tgtttttgtt
     4741 aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat ggtcgtcaat
     4801 ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga tttttctttt
     4861 acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc ttgctcatct
     4921 ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt tgtcacattt
     4981 tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc gtcttccaca
     5041 ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat agcgaaagta
     5101 acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc gtcgttttga
     5161 ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa tgtaataggt
     5221 gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat aaaaaagaga
     5281 acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca cctgcttttt
     5341 tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct aaaaagacaa
     5401 agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg atcgtcaact
     5461 tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg cctaaactaa
     5521 tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta taatccaatg
     5581 attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag tgactttggg
     5641 catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta tagatggtat
     5701 aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct gctttgactt
     5761 tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc atattttttg
     5821 ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact ggagtaagcg
     5881 aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct tttccatcat
     5941 atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta tagacatcag
     6001 atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct agtccgcttt
     6061 ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact tcaactccct
     6121 tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata ctgagatcat
     6181 tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg atcggtacaa
     6241 cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt gcatttgtct
     6301 tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg attacatttc
     6361 catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt tctatcgctg
     6421 aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg tcttgtgcag
     6481 cacttccgta agtaagattc attattttag aaggagcaat catctgtcca aaatagtctt
     6541 gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg acgttacttt
     6601 ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact gctgttggag
     6661 tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac gttccgattt
     6721 ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca ttgacctgaa
     6781 ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca acgatctgcc
     6841 catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata ccgattgaaa
     6901 aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta aaagaataac
     6961 ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc acccccatat
     7021 agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata aacagattat
     7081 ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt aacgcagttt
     7141 tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca tatatcactg
     7201 tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca gtactaaatg
     7261 tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct gctgtgattt
     7321 ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc tcgttgttgc
     7381 tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta tttgtctcct
     7441 ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa atacttgata
     7501 agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt aagtattttc
     7561 ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt tggattttat
     7621 tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca aataatcctt
     7681 tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag tttggggctt
     7741 tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa gattttattt
     7801 ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa cttgattaat
     7861 tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag gacgggatta
     7921 tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga gaaatgtgtc
     7981 gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caattagctg atcttgtatt
     8041 cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc taacgctcag
     8101 gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt ttcaagaacg
     8161 aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga ggtacgtttt
     8221 ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca tccaattgat
     8281 tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac tgcgaatcgc
     8341 tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt tgtattatag
     8401 atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca tacttgaact
     8461 tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct gatatgcccg
     8521 accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt gttcgacata
     8581 atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg tataagtcac
     8641 tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt ttgttgaact
     8701 tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca tcttttcttg
     8761 ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac tccatttatg
     8821 ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat tatcatagtg
     8881 ctcttttttt tactactact tataggtaga tttattctaa tccattattc tcccctacca
     8941 ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa aaagaaccct
     9001 tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat ttctttgata
     9061 cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct gactacatat
     9121 tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc tttcaccgtt
     9181 tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat ttgacgttaa
     9241 gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt tcaagacttc
     9301 ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat gagtatgaaa
     9361 tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta catactctcg
     9421 acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg tttcttctat
     9481 aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt gtagcttttc
     9541 cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag tagtttcaat
     9601 tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa tttcatttct
     9661 tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc agccagtgta
     9721 aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc cataattcaa
     9781 aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta tggcttcttt
     9841 taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg gattttcact
     9901 tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa taagtgattg
     9961 taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct ttaaggtaaa
    10021 aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac caatccatcg
    10081 aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt gttcatttaa
    10141 cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat tgcttgcaac
    10201 aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt gcgcctcacg
    10261 ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg tgatcctacc
    10321 agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat tcctcctatt
    10381 ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt catcattgta
    10441 ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat ttttctcgat
    10501 agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa tagtgtcctc
    10561 gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc agtgtactat
    10621 cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt aaatgactga
    10681 ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa tagaactcta
    10741 tttccagaga attaacattt gttttaactt ccaactccat cacccttctc tctcaaaatc
    10801 aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac tccatcaaaa
    10861 cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata aaaataatta
    10921 aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga atcaacttga
    10981 gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat cttgccgatg
    11041 ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa atatggaaat
    11101 cctagctctt ccaaagccat ttgtcgttgg taccaaacac tgccgagact tttcccaata
    11161 atatcgccaa tttcacgatc attaagaccg atgaagaagg acataaaaat gatttcttgt
    11221 ctttgactag gtaatgctgc aatcgcattt gccaactcct cattggaaat aaagattttc
    11281 ataccaagga caataaaagt accactacta ttcaaatttg gttccaaata atagaactct
    11341 gtacctaacc ctttttgttc caatatttcc attgatattt gccgttggcg aaatctagcg
    11401 tacttctttt gaatatttaa cgcctcattg cagactactt tcttacaaaa actgtcaaac
    11461 gtatgttgaa tgtgggtttc ctctgagtcg cccataatat tctccccctc aaaaaattca
    11521 ttcaactcgt tttacttcta tccctttcca ttattactac aatctagga
//
LOCUS       DAZBTH010000081        11446 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000081 DAZBTH010000000
VERSION     DAZBTH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.083"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHGNW_002079"
     CDS             185..1183
                     /locus_tag="ACHGNW_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6926354.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHGNW_002080"
     CDS             1295..1804
                     /locus_tag="ACHGNW_002080"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN6926355.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHGNW_002081"
     CDS             complement(1834..2493)
                     /locus_tag="ACHGNW_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN6926356.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHGNW_002082"
     CDS             complement(2495..3259)
                     /locus_tag="ACHGNW_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN6926357.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHGNW_002083"
     CDS             complement(3267..3944)
                     /locus_tag="ACHGNW_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN6926358.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHGNW_002084"
     CDS             complement(3981..5366)
                     /locus_tag="ACHGNW_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN6926359.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHGNW_002085"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHGNW_002085"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHGNW_002086"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHGNW_002086"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN6926360.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACHGNW_002087"
     CDS             6305..7162
                     /locus_tag="ACHGNW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926361.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACHGNW_002088"
     CDS             complement(7243..7578)
                     /locus_tag="ACHGNW_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926362.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACHGNW_002089"
     CDS             complement(7645..9015)
                     /locus_tag="ACHGNW_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN6926363.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACHGNW_002090"
     CDS             complement(9093..9629)
                     /locus_tag="ACHGNW_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6926364.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ACHGNW_002091"
     CDS             9938..11419
                     /locus_tag="ACHGNW_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926365.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2302 c         1854 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattc
//
LOCUS       DAZBTH010000082        11360 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000082 DAZBTH010000000
VERSION     DAZBTH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.084"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..507)
                     /locus_tag="ACHGNW_002092"
     CDS             complement(<1..507)
                     /locus_tag="ACHGNW_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6926366.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWAL"
     gene            complement(519..1211)
                     /locus_tag="ACHGNW_002093"
     CDS             complement(519..1211)
                     /locus_tag="ACHGNW_002093"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN6926367.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1230..1514)
                     /gene="macP"
                     /locus_tag="ACHGNW_002094"
     CDS             complement(1230..1514)
                     /gene="macP"
                     /locus_tag="ACHGNW_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN6926368.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1527..2090)
                     /locus_tag="ACHGNW_002095"
     CDS             complement(1527..2090)
                     /locus_tag="ACHGNW_002095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6926369.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2318..2980
                     /locus_tag="ACHGNW_002096"
     CDS             2318..2980
                     /locus_tag="ACHGNW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN6926370.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3104..4300
                     /locus_tag="ACHGNW_002097"
     CDS             3104..4300
                     /locus_tag="ACHGNW_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN6926371.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4401..5777)
                     /locus_tag="ACHGNW_002098"
     CDS             complement(4401..5777)
                     /locus_tag="ACHGNW_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6926372.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5814..6680)
                     /locus_tag="ACHGNW_002099"
     CDS             complement(5814..6680)
                     /locus_tag="ACHGNW_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN6926373.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6769..7794)
                     /locus_tag="ACHGNW_002100"
     CDS             complement(6769..7794)
                     /locus_tag="ACHGNW_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6926374.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7812..8486)
                     /locus_tag="ACHGNW_002101"
     CDS             complement(7812..8486)
                     /locus_tag="ACHGNW_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6926375.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8670..9563)
                     /locus_tag="ACHGNW_002102"
     CDS             complement(8670..9563)
                     /locus_tag="ACHGNW_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6926376.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9637..11334)
                     /locus_tag="ACHGNW_002103"
     CDS             complement(9637..11334)
                     /locus_tag="ACHGNW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6926377.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3369 a         2504 c         1954 g         3533 t
ORIGIN      
        1 caatgcccat tcatgtccaa cgggatcgaa acttgccacg gcatctttga aaatatggag
       61 tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac
      121 gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc
      181 actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc
      241 ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg
      301 cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc
      361 aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc
      421 aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt
      481 gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat
      541 tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt
      601 gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac
      661 tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa
      721 ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc
      781 aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa
      841 tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctggttt gtagccaaat
      901 cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt
      961 aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt
     1021 tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg
     1081 atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat
     1141 aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata
     1201 ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca
     1261 cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc
     1321 ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga
     1381 taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt
     1441 tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc
     1501 ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga
     1561 atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg ttgcgcctct
     1621 tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg
     1681 accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa
     1741 agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat
     1801 tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt tccagctgga
     1861 atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct
     1921 tgttcgtcaa aagcaatgat cccgacacca ccaggatgaa ataccagttc tctcttcgaa
     1981 gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag
     2041 atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct
     2101 gctttctttg taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt
     2161 attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc
     2221 cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa
     2281 ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg
     2341 gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct
     2401 aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa
     2461 aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa
     2521 aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa
     2581 tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct
     2641 ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa
     2701 ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac
     2761 aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact
     2821 agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa
     2881 attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt
     2941 aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa
     3001 gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat
     3061 tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac
     3121 tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt
     3181 ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga
     3241 gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa
     3301 acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc
     3361 tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga
     3421 tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc
     3481 tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca
     3541 atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc
     3601 agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct
     3661 ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt
     3721 gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac
     3781 tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga
     3841 taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat
     3901 ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac
     3961 agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga
     4021 tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc
     4081 agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga
     4141 tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg
     4201 tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga
     4261 agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact
     4321 aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct
     4381 tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca
     4441 aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc
     4501 agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag cggaacgaat
     4561 ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct
     4621 agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc
     4681 aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc
     4741 ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg
     4801 acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt
     4861 aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca
     4921 aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc
     4981 gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca
     5041 tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa
     5101 gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc
     5161 tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca
     5221 aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg
     5281 atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc
     5341 ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct
     5401 tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc
     5461 aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat
     5521 ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg
     5581 acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc
     5641 gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc
     5701 aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc
     5761 ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact
     5821 gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc aacaataaca
     5881 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
     5941 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
     6001 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
     6061 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
     6121 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
     6181 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
     6241 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
     6301 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
     6361 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
     6421 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
     6481 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
     6541 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
     6601 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
     6661 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
     6721 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
     6781 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
     6841 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
     6901 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
     6961 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
     7021 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
     7081 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
     7141 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
     7201 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
     7261 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
     7321 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
     7381 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
     7441 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
     7501 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
     7561 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
     7621 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
     7681 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
     7741 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
     7801 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
     7861 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
     7921 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
     7981 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
     8041 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
     8101 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
     8161 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
     8221 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
     8281 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat
     8341 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
     8401 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
     8461 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
     8521 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
     8581 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
     8641 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
     8701 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
     8761 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
     8821 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
     8881 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
     8941 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
     9001 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
     9061 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
     9121 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
     9181 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
     9241 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
     9301 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
     9361 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
     9421 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
     9481 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
     9541 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
     9601 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
     9661 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
     9721 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
     9781 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
     9841 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
     9901 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
     9961 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    10021 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    10081 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    10141 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    10201 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    10261 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    10321 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    10381 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    10441 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    10501 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    10561 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    10621 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    10681 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    10741 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    10801 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    10861 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    10921 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    10981 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    11041 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    11101 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    11161 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    11221 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    11281 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    11341 ctccccgttc tttttttatt
//
LOCUS       DAZBTH010000083        11238 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000083 DAZBTH010000000
VERSION     DAZBTH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.085"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHGNW_002104"
     CDS             6..305
                     /locus_tag="ACHGNW_002104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6926378.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHGNW_002105"
     CDS             378..1739
                     /locus_tag="ACHGNW_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN6926379.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHGNW_002106"
     CDS             1761..2021
                     /locus_tag="ACHGNW_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926380.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHGNW_002107"
     CDS             2035..2787
                     /locus_tag="ACHGNW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN6926381.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHGNW_002108"
     CDS             complement(2881..3486)
                     /locus_tag="ACHGNW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926382.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHGNW_002109"
     CDS             complement(3539..4513)
                     /locus_tag="ACHGNW_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN6926383.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHGNW_002110"
     CDS             complement(4891..5610)
                     /locus_tag="ACHGNW_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6926384.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGNW_002111"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGNW_002111"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6926385.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHGNW_002112"
     CDS             7161..8558
                     /locus_tag="ACHGNW_002112"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6926386.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHGNW_002113"
     CDS             8639..9079
                     /locus_tag="ACHGNW_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6926387.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHGNW_002114"
     CDS             9227..9553
                     /locus_tag="ACHGNW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6926388.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHGNW_002115"
     CDS             9554..10303
                     /locus_tag="ACHGNW_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN6926389.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHGNW_002116"
     CDS             complement(10383..10838)
                     /locus_tag="ACHGNW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6926390.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3565 a         1961 c         2303 g         3409 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatt
//
LOCUS       DAZBTH010000084        11088 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000084 DAZBTH010000000
VERSION     DAZBTH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.086"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..2085)
                     /gene="metG"
                     /locus_tag="ACHGNW_002117"
     CDS             complement(76..2085)
                     /gene="metG"
                     /locus_tag="ACHGNW_002117"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN6926391.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2353..2631
                     /locus_tag="ACHGNW_002118"
     CDS             2353..2631
                     /locus_tag="ACHGNW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926392.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2728..3549
                     /locus_tag="ACHGNW_002119"
     CDS             2728..3549
                     /locus_tag="ACHGNW_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6926393.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3834..4844)
                     /locus_tag="ACHGNW_002120"
     CDS             complement(3834..4844)
                     /locus_tag="ACHGNW_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN6926394.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4856..5809)
                     /locus_tag="ACHGNW_002121"
     CDS             complement(4856..5809)
                     /locus_tag="ACHGNW_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926395.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5799..6869)
                     /locus_tag="ACHGNW_002122"
     CDS             complement(5799..6869)
                     /locus_tag="ACHGNW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926396.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6875..7918)
                     /locus_tag="ACHGNW_002123"
     CDS             complement(6875..7918)
                     /locus_tag="ACHGNW_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926397.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7919..8860)
                     /gene="opp3b"
                     /locus_tag="ACHGNW_002124"
     CDS             complement(7919..8860)
                     /gene="opp3b"
                     /locus_tag="ACHGNW_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6926398.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8944..9291
                     /locus_tag="ACHGNW_002125"
     CDS             8944..9291
                     /locus_tag="ACHGNW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN6926399.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9361..11022)
                     /locus_tag="ACHGNW_002126"
     CDS             complement(9361..11022)
                     /locus_tag="ACHGNW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6926400.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3111 a         2269 c         2030 g         3678 t
ORIGIN      
        1 aatttacgaa aattgcttct caaatttcgg aaaaaggggg tatgacacaa ctagtgtcat
       61 accccctttg aatttctatg cgatttctga gccatttggc atggattttg gtgcttcgat
      121 tacttgtaaa gagccatctg gtgcttctgc agaaaggatc attccttgac tgatttgtcc
      181 acgcatcttt cgtggtttca gatttgctac aatgacgact tttttcccga tcaattcgct
      241 tggatcagga taaaattcag cgattcctga caagatttga cggtcttggc tgtctccagc
      301 atctagtcgg aattgcagca acttatctgc cccttctact ttttgacagt tgatgacttc
      361 tgctactttt agttctactt tttcaaatac atcaaatttg atttgttttt ctttcgttga
      421 aacaagttct gtttcttctg gatcccattt gactgtatct tcgtttgttt gagtaccttc
      481 agacattttt tcttgaataa aagcaacttc ttcttccata tctaaacgtg ggaagattgg
      541 tgtacctttt gcaactactt ttgttcctga aggaaattcg ccaaagtgta atccttcaag
      601 attcattgtt tcagagtcca atcctagttg attgaagatc ttctctggcg tttctgtcat
      661 gattggctga agtaatatcg ctgcgatacg caaagattca gctaaatgga tcatgacact
      721 attcaattca gctttctttt cttcgtcttt tgctaatacc catggttctg tttcatcaat
      781 gtatttgttt gcacgagaaa tcagcaccca gatttcagcc aatgctgtat tgaattccat
      841 tttttccatt gcatcatggt aacgtccgac aacgttagca gccgtcgtag acagttcact
      901 atcaaatgga gtgacaagtg aggcatagtc aggaacgagt ccatcacaat atttgttgat
      961 catagcaatc gttcgattca ataagttccc tagatcattt gccagatcat aattgacacg
     1021 ggaaacaaaa tcttctggcg taaagacacc atctgaccca aacggaacag cacgaagcaa
     1081 ataataacgg agtgcatcta aaccataacg ttccacaagc atttcaggat aaacgacatt
     1141 tccttttgat ttagacattt ttccgtcttt catcaatagc cagccatgcc caaatacttt
     1201 tttaggtaga ggcaaatcaa gggccatcag catgattggc caatagatcg tatgaaagcg
     1261 aacgatttct ttaccgacca tctgaacatc tgcaggccag tatttttcga atagactttc
     1321 atcttcggag ccatatccta gtgcagtaat ataattggat aatgcatcga tccatacata
     1381 caccacatgt ttcggatcac tcttgactgg tactccccaa gtaaaagttg ttcgtgaaac
     1441 tgcaagatcc tctaatcctg gtttgataaa gttgttgatc atttcgtttt tacgagattc
     1501 tggctggatg aattcgggat gttcttcata gtattccaat agacggtctg cgtatttgct
     1561 catgcggaag aagtatgatt cttcttttac caattctact tcgtgtccac ttggcgcttt
     1621 accaccgatc acgttacctt cgtcatcttt gtacacttct gcaagttgtg tttctgtaaa
     1681 gaattcttcg tcagaaacag agtaccagcc ttcatattca cctaagtaaa tatctccctg
     1741 ttctaataaa cgatcaaata ttttttgaac agctgcttta tggtaatcat ctgtcgtgcg
     1801 aatgaattta tcataactga tatcaagcgt tttccataat ttcttgacat cagccgccat
     1861 tttatctaca tattcttgcg gttttacacc tagttcttct gcttttgttt cgattttttg
     1921 accatgttca tccactccag tgagataaaa cacatcaaag cccatcaacc gtttataacg
     1981 agcaattgcg tcacatgcaa tcgtagtata cgagttgccg atatgaagtt tgccgcttgg
     2041 ataatagatt ggcgtagtga tataaaaagt ttccttttct gccacggggc tcttcctcct
     2101 ttatcgcatg atgcgaattc gtttccttgc tagtatacca taaaaaaagc atgctgttag
     2161 ctttttttca aaatgaaaaa gactggataa acgacttcta tctacgttca atctatcctt
     2221 taaaaggatg aataggttga ataaatgcgg gtttttgttt tgtttcatat ctactaaaaa
     2281 acggcttttt atttctcccc tcacacgtgg tataataaag actatctatc ttagtaaggg
     2341 actggaacaa tcatgcagaa gaaacatctt ttttttacac ttagtattgc ttttctttca
     2401 ttagcacatt taatattcag ctatttttat attcgtatgt atggttattt taacctccat
     2461 gggcatttga acagttttat gacagcagct tggattttgc gttttatcat tgacgtatat
     2521 atcgttattt gtggcttctt tgccattcgt gaagagcggt ataaagtatt gccgttttat
     2581 ctgcttttct ttttattcaa tctcatactt ccatttattt ttcatattta aatgataacc
     2641 tagatttttc aagtaaatcc ttggtttttt ttcagagtct gctacaataa ggtaaaaagt
     2701 acttttactg tcaatggagg tggtcagatg aaccgttttg cttctaaaga agaagaaaaa
     2761 ttatattatg aacataaagc aagtgaagaa gaatttctta actggtatca aaaacaggaa
     2821 cgtccagaat atgacaagcc ctctttgact gtcgatatcg tactgatgtg ctataacaaa
     2881 gaagaagatc agctgaaagt tttgttgatc caaagaaaag ggcatcctta tcgaaattca
     2941 tgggcattgc ctggtggctt tgtccaaaag gatgagtcaa ctggagaaag cgtcttaaga
     3001 gaaacaaagg aagaaactgg tgtggttatt tcaaaagaaa atattgagca actgcataca
     3061 ttcagtacgc ctaatcgaga tccgcgaggc tgggtcgtca cagtcagcta cttagccttt
     3121 attggagaag aaccgctaat cgccggagat gatgccaagg aagtacgctg gttcacacta
     3181 gaacgtcata gtaacaagat ccatttatct agcggagaag tggctatctc gcttgattta
     3241 gtcactggag agtcttctgg caaagatact ttggcttttg accatagtca aatcatcttg
     3301 aaagcattca accgtgtagt caataaaatg gaacacgagc cgcaagttct acaagtttta
     3361 ggtaaagatt tcacgattac ggaagctcga aaagtatttg ctaaattctt agggatcgat
     3421 tataaaacga tcgatcattc caattttaag aaagctatgc tccaatattt cgatgagatc
     3481 ggtgaacgtc ctgtaggaat cggccgtcct tcaaaaatct atcagctcaa gccggggcac
     3541 catgaataag gttgtgagca aaagattcag acctaatcat tcaagaaaaa acgcaaaaac
     3601 aactctttgt gagttacgtt ttttgtttca gaattttgat gtattcgtaa actttctgaa
     3661 taaaaaatta acaatagcag aaaaagctcg tacgaacttt ttctgctatt gttttattgg
     3721 tttgtcataa ttagtgagct tttcttcaaa agaaaaattc ctagaaacaa aagtcattaa
     3781 gactttttat taacaaggct aaccctaacg ctcctatcag tttcattcat tttttatccc
     3841 ttgataaccg aatgtctgcc cattttttct actacttggc tctttcttac ggaattgctt
     3901 tcttctgctc cgtcggtatt gatcaaaaga aattttttat tataccattg ggacttataa
     3961 cgcaattttg ttattaattt atgccagtcg tgaatagaaa aatcagcatg tgcctcttct
     4021 tttggaaaag attctacaaa taagtagtca tattgtttga ccaatttccc agataattgt
     4081 tcaagataat cttctcgctg gttcttttgt ttcagataaa gatccattac ttttctcttt
     4141 tgtttctgat aattgcttgc ctgatctaac ctgatctttc tatggtgagc ggagcggact
     4201 ttcacattca atttccgctg tgcatgttgc agcttttctt ctgtctggtg aaattttggt
     4261 aaagttactt ctagaggtcg agatgtttgg atcaatgtct tcggatgata ggcgatcccg
     4321 atccatttag ctaaagattc tttttttact ggcgaaacat cacaaagtag tgcgacataa
     4381 aattcttcat tatatcgagc agaaatcgta gcggagcgaa tcgatccttc aactggccga
     4441 tgctgattta tcttgattag ttctttttgt tttggtaact ttaagtagcc attttccaga
     4501 tagatcgtac gggtttggtt gttggtcgta taagattgcc acatactttt tttcgtttta
     4561 agctttggga agctggcacg tcccttgaaa taattgcgaa aagcccgatc caaattaagc
     4621 tgtgcatttg ctaaagcaag actgtctact tcttttaaga atgggtattc tttttttaat
     4681 gtagcgggtg tgagcgaaaa attggctact cctgtttgtt gccaactttt ctgccgcttt
     4741 ttcagcaaat ggttataggt aaatcgagca catccaaaag tacgaatcaa ttgctgtttt
     4801 tgttcttctg tcggatacat acggaattta aaagctttta gcatctatgt ccctcctagt
     4861 tagcaaccag aagagattcc ttttgtcgtt ttagtttttt tgcgtaatat gggacttcct
     4921 gctcagttgc atagacaaaa tgttctggtg cgatttcacg aagttgacgt acttgtccgt
     4981 catctggttc aggttgatat ttgattcttc tgcgttttct ctcatggtct ggatctggta
     5041 gaggaatagc agataacaaa ctttctgtat aaggatgaac accgtgatta tagatttcat
     5101 cacttgttcc catctctaat aacaagccgt tatgcattac accgattcgg tcactaatat
     5161 gtttaaccat cgacaaatca tgtgcaataa atagataggt caattgatgt tctttttgga
     5221 gatcttgcaa caaattgacc acttgggctt ggatcgacac atctagtgca gaaattggtt
     5281 catcacaaat gataaaacgc ggttttactg ccaatgctcg tgcaatacca atacgctggc
     5341 gttgcccacc tgaaaattca tgaggataac gagtaccgtg gcttggattc aatccaacag
     5401 ttctcaatag atcatcgact ttttttgcac gctcttcaga tgatttagct agtccattaa
     5461 catcaattcc ttcggcaatg atatctctga ctttcatacg aggattcaac gaagcgtatg
     5521 gatcttgaaa gatcatctga acatcatgcc ggaatttcgt caaacctttt ttatctttga
     5581 tcttcgttat atcttgaccg tcgaataata tctcaccatc agtaggttca ttcaagcgaa
     5641 tgatcgtacg gccagtagtg gattttccgc ttccagattc tccaactaaa ccgaatgttt
     5701 ctccttcata aatgtgaaag ctgatgtcat ttacagcatg tacttcattc ttttttccta
     5761 cattgaaata ctgtttcaat cctttaactt caagtagttt atttggcatc agcaattcct
     5821 ccttgaccgt aagtatgttt ttgtttctct ttaaagatag cccagcgacg ttggatttct
     5881 tctggcggtg tcactttggg tgcctgcgga gcaagtagcc aagttgctgc cttgtgtgtt
     5941 tttgatagtt caaaaaatgg tggttcttgt tctgcatcga ttttcatcgc gaattcattt
     6001 cttggataga atgcgtctcc ttttggcggg ttcaataaat ccggtggaga accaggaata
     6061 gcatacaatc gatcgttcgc actatctaat gtcggcattg aaccaagcag tccccaagta
     6121 tagggatgtt gcgggttgta gaaaatttct tccgatgttc caacttcaac gattcttcct
     6181 ccatacatga cagcgacacg atcagcgaca ttcgctacta caccaaggtc atgagtgata
     6241 aagataattg atgtatcgat ttttgtctga atttctttca gcagctccaa aatctgtgct
     6301 tgaatagtca catcaagcgc tgttgtaggt tcatcagcaa tcaggatttc cggataacat
     6361 accagtgcaa tcgcgatcac gatccgttga cgctgacctc ctgagaactg atgcggataa
     6421 ctgttgatac gtttctcagc ttcgggtatt ccaactaatt taagcaattc taatgcagct
     6481 ttttgtccct ctttttttga aactttatta tgctttctaa gggactcagc gacttgcttc
     6541 ccaatgggca tcgtcggatc taaagaggtc attggatctt ggaaaatcat cgctatttct
     6601 ttaccacgga ttttttgcat ctctctttct gattttccaa cgatatcgtt acctttaaag
     6661 aggatttgac cagcatctat attggcgttg cttgacagca agcgcatgat gcttctggtc
     6721 gtcactgatt ttccacttcc agattcccca acgatcgcca atgtttctcc tttattcaaa
     6781 tgaaaattga caccgcgaat cgctcgtacc tttccagcat acgtatcaaa agagatttct
     6841 aagtctttca cttctaaaat tttcgtcatc gtcttcactc tttcatttta ggatcaaagg
     6901 catcacgcaa tccatcagca agtaagttaa agccaatcat gataaccgat aaagttactg
     6961 ccggaatcca taatagataa ggcagataca ggaatgtctt gtacccttcg ctcaacatcg
     7021 ttcctaatga agctgacgga ggcgtaagac ctaaaccgat aaagcttaaa aaggcttcga
     7081 agaaaatagc agatggaata ctaaacatca tttgaataat aatgacacta gagatattcg
     7141 gcaaaatatg tttgaatgca attttcattc ttgattcacc taatgtcaat ccagctaaaa
     7201 catattcttg atcttttaat ttcattgttt gtgcacgaac gatacgtgcc attggtatcc
     7261 agtttgtgat ggccatagca gccacgattg aaaaaattcc tggttcaaaa acgaccaaca
     7321 tcaaaatcat gacaacgagg tttggaattc ctgataagat ttccaaaaaa cgttgcatca
     7381 cgttatcgac acgtccgcct aacaaaccag aaaccaaccc gtaagctaca ccaatcgtta
     7441 catctagtaa tgcagcaata aatgcgatca gcaacgagat tctcattccc ataaataagc
     7501 ggcttaatac atcacgtccc aaaccgtctg ttcctaaaaa gaaatttacg tttgaaggga
     7561 cgtttgcttg agcatattta tcaacaagtt caccaccaac cattgctttt ccattcaatc
     7621 catcgatatt gattcccgga atacgaggag gcaaattgat ataagccacg ttttgggcag
     7681 ttggatcatg aggagaaaca aagattgtga tcacagagat caaaatgatg accaatagta
     7741 aaaccattga aataacggca gctttgtttt tctttaaacg gcgccatgaa tcttggataa
     7801 aattcaaaga aggtgcagta atctgttcac gttcttcttc ggtatttttt tgtaatggaa
     7861 gaaattcatc cgcagggata tctgcaactg tttcataacg ctttggtatc atttccattt
     7921 atcctcggct cccttctgaa acacgaattc ttggatcaac tagaccgtac aatagatcca
     7981 cgatcaaaat aacaaaaatc agcataaatg agtaaaggat cgttacagcc atgatcgtag
     8041 gataatcatt tgttgtgatt gacttaacga attgttcccc gataccagga atagcaaaga
     8101 tattttctac caccagtgat ccggtcatca atgctacagc taacggtcct aataaagtca
     8161 tcaatggaat caaactattt cgaagaccat gtttaaaggc gatttcccaa cggctcaatc
     8221 cttttgcttt tgctaattcc acataatcac tatgcagtac ttccaccatt tccgtacgga
     8281 taaaccgagc agagtcagca agcggcgaca tggccaacgc aattgttggt aagattgtgt
     8341 acacaaagcc atcccatttt gcgattggca aaacttgtaa tttgattgca aagatatatt
     8401 gtaataaaac agcaaataca aagtttggaa tggaacgacc taatatcgct acgagtgtag
     8461 aagatgtatc tgcccatgta ttttgcttca tggcagagat cgttcctaaa atcgtcccta
     8521 ggactgttcc aaagataatt gcctgtaacc ctaactgcaa ggatggtccg attcttccag
     8581 ctaacaaatg tgcgactggc tggtttttaa attggaagga aattccaaaa tctccttgaa
     8641 gcaaattaga aagataaatc ccatattgta cgataactgg tttatccaaa ccgacttgtt
     8701 tatttagcat ctcgattgtt tcaggactca aacgttcctg attcgtatat ggcgtgcccg
     8761 gcagcaattg catcaagaaa aaggtgattg ttgcaattaa ccaaagcgtg atgatcatga
     8821 aaaagacacg ttttaataca tatttgatat aactattcac taatgtttcc ccctagcttt
     8881 tgacttctga cagatgtttc gctacattat tagataatga accaaaaata aaggagcaat
     8941 caaatgaaaa aaaaatctat cccctacgca gtagcttttc tactgattct ggttatttta
     9001 ataaaaaatc tcatcaacca ttcctttaca ctcattcaat tatctaatga tctattctta
     9061 tggtcattgc cgtttctgat tatcggcggt tttttatggg tcttttcttc gggtttcttc
     9121 gatcattttc agcgatcggt ccatcttgca cgaacaagga accgaaagaa aaaaccagaa
     9181 ttttcctcgc tctcgtctgc cagctacggt atgtacagct tttggctgat cattgccgga
     9241 atactaattg ctctttctgc tattttcatg cttttttctt tgttaggata agtgaatatg
     9301 agaacgaggt tgcgacattt gccgcagcct cgttttttct catgctcacg gtattaacga
     9361 ttattcttca acgtatgtcc atttgtaatc ccatgaagcg cctgccgcat ggtgaacgat
     9421 acctttgatt ttttcattca ctaggtgacc ttctgctttt tggtacaccg gaacgacacc
     9481 catatcatca gagatgatct tgattgcttt ttggaaatca tcccaacggg cttgttcatc
     9541 gccagcgtct gttgatgaag ctgctttaac tgcttcgtca tattctgcat tgctccagcg
     9601 accacggttg tatgaattac ctgtgacaaa caaatcagta aagctgcttg ggtctgcata
     9661 gtcagcagcc catccgccta gtactgcgtc aaaatcaccc gaagttgagc ggtcaagacg
     9721 tacagagaat ggaacaggtg ttggtttgac tgttacgcca tccaagtttt cttggattga
     9781 gttttgaaca tattcgatta cttttttcga tgaatcatca tcagaagcca tcaaatcaaa
     9841 ttctaaagaa tcaacgccca attctttttt cgctttttcc caatattctt ttgctttatc
     9901 tttgtcataa gaaactaatt ttccagcttc ttttgcaaag tcttcgttcg ttgttggact
     9961 ttttaccata tctgatggga tcaatcctgt agagacaact gatccgtcac ctagtacttg
    10021 ttttactaaa gcttcacgat cgatagaata agaaatagct tttcttaggt tcacattgtt
    10081 gaatggtgaa ttcttttcac gttgattgaa ttcgatatag cttgtccgcg cttctttaac
    10141 agaagtataa gctggatcgt ttgcgttttg ctgtgctaac tcaccagaca agatcacgtc
    10201 atctgcttgg ccatctttga ataagttcaa tgcagtagaa gattctttta ctacactgac
    10261 tttgatttca gaaagtttta ctgtatcttt gtcccagtaa tgatcatttt tcttataagt
    10321 ccattctgta tctgttcctg gtccatcaaa atcttctaat gtgaatggtc cattataaac
    10381 agctttatca cttgcagatg cataggcatc cccattttct tccactacgg attgtttttg
    10441 tgggaaaaat gatgggaaag caagcagata ttggaaatat ggtgtttgtt tcgtcaattt
    10501 gatttctaat tcatagtcac caactgcttt gatccctagt tctgatttat ctttctttcc
    10561 agctgtaatg tcttctgcat tttcaactgg cgcatacagg taagcatatt cagaagctgt
    10621 agctgggtct actgttcgtt gccagccata aacgtaatcc gctgctgtaa caggatcacc
    10681 atttgaccat tttgcgtctt ctcgtaattt gattttgtaa gttaatccgt cttcactgac
    10741 ttctgccaac tcgctagctc ctgccggctg tggtttgctg tcttcatcaa gacggtaaat
    10801 tccttcataa acgttattca atgctgtaaa actgattgta tctgttgcaa ctgataagtc
    10861 agctgtaggc atttcttgag gcacgactaa gttaaatacg cctcctccat ctgctgaacc
    10921 gctttttgct gaactactgc ttgtggctga actgccgcca taacatccag caagtacaag
    10981 gccgcatacc gcaaccaaac caaatgtaaa tttctttttc attttacgta cccctttatt
    11041 ggaattttca gaataattaa tattttctga agttgtttaa gagtttaa
//
LOCUS       DAZBTH010000085        10994 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000085 DAZBTH010000000
VERSION     DAZBTH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.087"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..170)
                     /locus_tag="ACHGNW_002127"
     CDS             complement(<1..170)
                     /locus_tag="ACHGNW_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6926401.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRG"
     gene            complement(181..1557)
                     /locus_tag="ACHGNW_002128"
     CDS             complement(181..1557)
                     /locus_tag="ACHGNW_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6926402.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(1686..2333)
                     /locus_tag="ACHGNW_002129"
     CDS             complement(1686..2333)
                     /locus_tag="ACHGNW_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN6926403.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2417..3844)
                     /gene="uxaC"
                     /locus_tag="ACHGNW_002130"
     CDS             complement(2417..3844)
                     /gene="uxaC"
                     /locus_tag="ACHGNW_002130"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6926404.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(3918..4997)
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002131"
     CDS             complement(3918..4997)
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002131"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6926405.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5000..6631)
                     /locus_tag="ACHGNW_002132"
     CDS             complement(5000..6631)
                     /locus_tag="ACHGNW_002132"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6926406.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6948..7619
                     /locus_tag="ACHGNW_002133"
     CDS             6948..7619
                     /locus_tag="ACHGNW_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6926407.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(8455..9909)
                     /locus_tag="ACHGNW_002134"
     CDS             complement(8455..9909)
                     /locus_tag="ACHGNW_002134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926408.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(9910..10209)
                     /locus_tag="ACHGNW_002135"
     CDS             complement(9910..10209)
                     /locus_tag="ACHGNW_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926409.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(10512..10883)
                     /locus_tag="ACHGNW_002136"
     CDS             complement(10512..10883)
                     /locus_tag="ACHGNW_002136"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6926410.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         3494 a         2245 c         1584 g         3671 t
ORIGIN      
        1 ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt ttccctatga
       61 tgaacatcaa aaatctctgc agcaattggc ccgaaaaaga gattgtgttt ataagtcatt
      121 gtatccatga cacaaccatc tgaatcaata caaaatatcc aactgttcat gttttttctc
      181 ctagttatgt ttttgattta gcaaattata attttctaat actttttctt tagataatgg
      241 atatacgaaa aataaaatca atcctacaac aaagaaacaa actgctggaa tcaacgttga
      301 agagttatac agcttttgta aaacttctcc actttgtact tttgctaacg aatcataccc
      361 tattatagaa agagtccaac ctccgacacc gccagctaaa gcttgtccaa tttttcgggc
      421 aaaagaatat actgcataaa tagttccatc ttctctttgg ccagatttca cttcttgatc
      481 atcgattaca tcagtaatat tcgcccaaat gactgtatta aaaataccta accccatata
      541 tccaacagaa gtaagaacta agaatacata catattacta ggctttatca aaaataaaag
      601 taaatataca atagcagaga agaacatccc tactgacgcc aattcttttt taccgtattt
      661 tttactgaaa attaaactta caggtgaaac aagtaccaac atcaaaatag gattgataaa
      721 gttcattaaa actattccct gtgcattatt ataataatct ggaaatacat aattgttcat
      781 actcattgtt aaaagctgag caagcaacaa aaaaatagct gcagctatta tagaaagcaa
      841 cgctctattt ttagctaatc cttttaaaga tttcaacaat gaaactttta actgcgattc
      901 atcactttta acccgttcta cagttaattt ataacatatt acgtaacaaa taactgcaca
      961 cactgaaaat actcctgcca caattgtaaa agttgatcca ccacgtacaa tttgagctcc
     1021 agaactatct cttgtataaa taaaaattgg tgttaacacg ccaatgaaca tacctgctaa
     1081 ggtagctcct atactacgaa atgttgataa cgctgtcctt tcttctgcaa cagaagtgat
     1141 agctgatgcc attgaaccgt aaggaatatt aatagatgta taaaaaatac ttccccatag
     1201 tatatatgtt acatacatat atataacttt aatactcata gaagctgatg ccattgatgt
     1261 ttgatacatt aaaaaacttg ctaaagcgac tggaccactc atccgtaaaa tccacggttt
     1321 aaatttgcca gttttagatg gttttgatcc atccacaata cgccccattg ttatgtctgt
     1381 aactgcatca ataaatcttg ctactaaaaa taatgttcct acggtagctc cgcttatccc
     1441 caatactttt gtataaaaaa tcattaaaaa agaactagca aaaatgaaag tgaaatcatt
     1501 cccaaaatct ccaaacatat atcctatttt gtctcttaga ccaaattttc tttccatata
     1561 atttgcgctc cttcctattg ttaagcgttt tcattctgtt gaactgccaa cttacgtact
     1621 atactctgaa gtataaagag ttagtattta ttttttacat tttatagtat tatttatttt
     1681 ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc tattcgagtt
     1741 acttcttcaa aatcaccttt ttcaacagga gccaacaaat tcccacctac accaactgca
     1801 acaacgcctg cttcaaacca ctcttctaag ttatctagac ttactccgcc agtaggcatt
     1861 atatttacat atggtaatgg agcttgatac gtttttacaa cagatggacc aaaagcagaa
     1921 cctggaaata gtttaatgat ttctactcca gcggtaagtg cctcctttat ctcagtaatt
     1981 gtcatacaac ctggtaaata aggaatttga tataaattac aaatctctgc tgtttgtcga
     2041 tcaaaagatg gacttacgat atattctgct ccagccataa ttgcaatacg agcagtaaac
     2101 gcatctaaaa ctgtaccggc tccaattatt acatcttttt gatcagcata ttttttagca
     2161 atttctttga ttatttcttc agcattcgga actgtaaaag ttagttcaat ccctttaatt
     2221 ccacccgcag taattgcatt tattgcttta atggcctcat ctttattttt tccacgtaca
     2281 accgcaatga ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc
     2341 tccttctttt atggatacat actctagcta gttgtactaa cttacaataa ataaatttaa
     2401 gcttttattt tttgtattat tttctacttt cgatttcaaa aaaattaaaa taattatacg
     2461 cattacgata acaaatattc tccactatct gactaagata catttcatca tctggaattc
     2521 gacctgcttt aacccactcg cttagtaatt gacataatac tcgacggaaa tactcatggc
     2581 gaggatagct tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac
     2641 tttgttgcgc catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc
     2701 aagcacatcc caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg
     2761 ctagtggaag ccaatcggtt ggatttaatg aatataaaat tgtttttggt aagcacccac
     2821 tcttttcagc agtgtcgaac aatgctacaa cattttgtgt caaactagct tgatccccca
     2881 tcgaatcacc accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta
     2941 aagcatttaa atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta
     3001 attgaatacc tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta
     3061 aagcttttat taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat
     3121 tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa ataatccact cgttggctca
     3181 atgcctgctt caacccatta aagtttttga ttggtatacc cgctgcttga ctaagtttta
     3241 aaatatagtt cgaaaatcct gatgatttga tactccagac cttatctgga cggaaagtag
     3301 gtaatacctt gaaagaatta tcctgtttag aaagctcagt atgatatcgc aagtcatctg
     3361 taggatcatc cgttgtacaa atggctttca catccatcat cttcagcaat tcttttgggc
     3421 gataggaatc cgtttggatt ttttcgttgg cacgtcgcca aatttctggt gcattcttct
     3481 cagaaattgt caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa
     3541 taggattacc tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat
     3601 tacctgcacc actgatcaac tcttcttcta tcccattagc tcgcatcaat cgccatttat
     3661 aatgatctcc agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat
     3721 tttctagcaa acatgctggg tctaaatgac aatgataatc gatgattggt tggtgttctg
     3781 caaatttatg aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa
     3841 acatatttaa gcactccttt ccttccctta gtttttagaa gagaaaattt ttcttctaaa
     3901 tagactccat tatataatta tttactcgcc cccttttcaa gcgcttccca aataccattt
     3961 aaataaactg cacctagagc tcggtcatat aacccataac caggtctacc ggtttctccc
     4021 caaatcatcc gaccatgatc tggacgaata tagccattaa atccattatc gtacattgtt
     4081 tttaatatct tatacatatc tagagaacca tattctgatt tatgcggcgc ttcataaaaa
     4141 tcgagtccat tatcacttaa atatttgata ttacgaacat gtgcaaaagg aatccgatcc
     4201 cgttgacaaa attcagatag aatttcatag acatcattat ctggtcgttc tgcaatactt
     4261 ccagtacata acgtaatacc attagaagga gaatccacta cattacacaa ccattctaaa
     4321 tcgtcacgat ttttaacaat acgaggtaat ccgaaaatag aatatggtgg atcatctgga
     4381 tgaactgcca ttttaatccc aacttcttca catgtcggga taattgcttt gagaaaatat
     4441 gcaaagttct ctcgcaattt cgcttcatca acttctttat aggcttctaa taattgcttg
     4501 atttgtgata accgatgagg ttcccaaccc ggcaattcaa aattattagt agaattttct
     4561 acttttctaa taatttcctc tggatcagta ggaatatttt ttgaaatata ggctaacgta
     4621 gaagacccat catctaaatg ataatccaaa tctgatttta cccaatcaaa gactggcata
     4681 aaattataac agataacctc aatcccaaat tctgccaaat tgcgaatcgt ctgtttataa
     4741 ttctcgatgt attgatctcg tgttggcaaa ccaattttga tatcatcatg aacattaaca
     4801 ctctcaataa ctttcaaggt aaggccattg gcttcgactt catcttttaa agctttaatt
     4861 cgttctctcg gccagacctc tccggctggt atatccatta atgttccaac aattcctttc
     4921 attgctggga tctgacgaat ttcttgtagt gagatttttt cttcatttgc cccataccat
     4981 ctaaaagtca tttccatagt tactttcctc caacttctgt tatatattta ttgattgttt
     5041 tacgaactgc ttgaggacct actagcattt cgcaaaagat tttttcaatt ttctttccta
     5101 tctcaacttg atataaatct aaaccaaata tttccttatt ttctaagatt tcctgtaccg
     5161 cacaatgaat ctttgcagga tcttgttcac ctaatttcaa tactgagaat ttttcctgca
     5221 actctaccaa taaaggatca ggacttggca caaaattttt tccttgatca tcgactgcca
     5281 ataaataacg taaccaagca gcaatagcga taggaataaa tgttagattt tctgcatttc
     5341 cgtctttttc gacatacttt ttaattgttt caccatagcg aatcgctact ttttgagaag
     5401 tatctgttgc aattcgttgc ggtgtatctg gaatataggg attaggcaaa cgtttattga
     5461 ttacttcatc aataaattgt tttggatcaa taacgcctgg actttcaact actggtaagc
     5521 cttctacata acctatccct ttaattaagc caaccaaatc atcatcttgc atttctgcct
     5581 gaattgattt ataccccaac aaacaacctg ttattgctaa tgccgtatgg agcggattta
     5641 aacatgtcgt aaccttcatg atatctgctt tatcaactgt ttctctatct gtcataatca
     5701 caccagccgc ttccagttta ggtcgaccat ttggaaaact atcttcaatg actagataat
     5761 ggactttttc agtattggta aaagcagcga tattggtatt tttcgcagta tgaattatat
     5821 ctaagtcttc gaaacccgcc ttcaataaat ccattttgac actttccgaa ggatttggcg
     5881 taattctatc aatcatactc caaggaaaag tcactttttc tttatccttt aaataagcaa
     5941 taaatttttc agaaacgtat cctttttttt gccaagcttc tgcaatcata aaaatgctat
     6001 cttgaaaacg ttgaccattc tgtgagaaat tatcagtaga aaccatcgca atcggtaact
     6061 ctccagcatt aaacctgcta tacaacaagg atgtcacaat actcatcgta tgttgaggat
     6121 tggtaggtcc agattttata tctgcttcca ctaccgaaaa aaattgtcct ttagaatctt
     6181 ttaaactgta tcctttttca gtaatagtaa acgttgtaaa ctgtaatgta ggatcttcaa
     6241 aatatttaac cactctttga tagtccgtta tattctttgg attgcaatat aaactatcag
     6301 ctgttactgc taaaagtctt tttttgagtt caccatcttc acccataaca atctccaaaa
     6361 tatcattgtt gtagtcatga taagcttttt ctactacttc ttcatcaaat gtctcacaca
     6421 caactactcc ggattctaat tcattttgat ctgctaagca ttgagcaatt tctgcatgaa
     6481 atcctcgata aagatttcct ccaccaaaat gaagccacac tggatgtttc tgaccttctt
     6541 gtttgaccgc ttcgacatta aaatacggta gttgaacttg cattttgttc aatccttcga
     6601 tagaatcttg ccagtcttta atcaaaacca tatttacccc tccatactga tatgttagtt
     6661 tccgttacaa ttgtagtata aaatcgaatg taatcgctgt caataagaaa aaactaaaaa
     6721 tattcttttt tctaccatgt cagaattatt gtgtcttttt ttagtaaaag cacaccaata
     6781 tatatttttt agaactaact taaattaaag cttaatttct ctttgatctt atttgagtat
     6841 tttttgtttt tttaatagct agtatgttac tttgaaattt gatattatat aacgagcata
     6901 gaactaaaat atcaaattca aaaatgaaat tttgagaggg attaaatatg acaattaaaa
     6961 ctagtctcca aaatcaagct tatcaatcta ttcgtcagca aattatctac gctgacttag
     7021 aacctggaaa aaaagtatca gaaaaaaaat tggaagaaac gctttctatc ggtcgtactc
     7081 caatacgtga agcattgatt caactccgtc atcaaggact tgttgataca attcctcaaa
     7141 gtggaaatta tatctcatta atcgacttag atttagcaaa aaatgctcgt tttgtcagag
     7201 aacatttaga acgacaaatt atgctagagt gttgtgccaa gatgaataat caaaagaaac
     7261 aagttttaga aactataatt gcagaacaag aaaaagctgt tagtcaaaag aatgctcgta
     7321 gtttttttca taatgataat ttatttcata aagcttgttt tgaaattgca gaacgggaag
     7381 aagtatggca atggttggaa gataataata cgcacttgga acgatttcgt tggttacgag
     7441 taaaaacgga aggattaaaa tgggaaaaaa ttatggaaca acatcatcaa ctatttaaag
     7501 ccttgaccat gcaagaccct gaagaagcta acttcttaac atccttgcat cttcatttaa
     7561 tgcttgatga acaaaatgtt gtattgacta ctcacccaaa atattttaaa aatatataac
     7621 tgctaaattt ctttttttta atttatttac atttctgatt actacagaaa actaactact
     7681 ttttttatgt aaagttctgt caaatatagt cactttatat tactttctct tgagtaaaaa
     7741 aacatgtgag gtcgtaaaca aagattgaac tatctacatt catactctat aatctgttta
     7801 tttgcagact gataatagca aaagaaaact tataatcaaa taatatcaac atttgatcac
     7861 ataatttgag attgaatgct gatttaccta tgttttacgt aatggtcttt agttattttt
     7921 gctcataaaa tgcataaaac acgcgttttt atgtgtgctt gaaatttctt ggctctataa
     7981 tttctaagac tgataaaaat attctatcgg ttctttttgc aaataaaaaa acatatagaa
     8041 gctccattag attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc
     8101 attctataaa actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag
     8161 aatatccgaa gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga
     8221 tgttcttata tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact
     8281 attacgagta ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt
     8341 caaatagttt ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag
     8401 tagcaactta attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt
     8461 ccaaatgttt ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata
     8521 agtcactagc ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag
     8581 cgacgattga tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg
     8641 ttctggtacc gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg
     8701 tgctaaaagc tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc
     8761 aagatagcat ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc
     8821 accaaggcgc tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc
     8881 cttttcccat agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa
     8941 tatagctgac cagaagctta gaggctctat attttttact actatcggca atgaagtttc
     9001 ttgtttttgc tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc
     9061 atcagctaaa gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa
     9121 catcgactga gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg
     9181 atcgccacca ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa
     9241 acgccgtagc attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat
     9301 cgctaaatgc caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg
     9361 tagttgttgt gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc
     9421 aggacaatcc cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt
     9481 ttcggttaat aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt
     9541 caaataaaaa taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag
     9601 ctcaacagtt actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg
     9661 ctgagtgtta cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa
     9721 ggtaccatca ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt
     9781 cagttcttgc ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt
     9841 ctcaggaatc gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc
     9901 atcaaacaat catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc
     9961 aaacatctgc tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt
    10021 tttaccttgc tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt
    10081 atgatgattc aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt
    10141 cgaatgctca agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg
    10201 gctttccatt aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg
    10261 agtagtttcc tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc
    10321 ctcctttacc aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat
    10381 attgcttcca ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca
    10441 acagattcgg ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat
    10501 gatgagcttc atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc
    10561 agtcggttgt aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc
    10621 ccagaaagat agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac
    10681 cactgtttct tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg
    10741 atcatctcaa ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa
    10801 ttgttattta aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga
    10861 attttttcat cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat
    10921 ctaaatgaat acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt
    10981 tacttactct aaac
//
LOCUS       DAZBTH010000086        10922 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000086 DAZBTH010000000
VERSION     DAZBTH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.088"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHGNW_002137"
     CDS             complement(<1..281)
                     /locus_tag="ACHGNW_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926411.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHGNW_002138"
     CDS             453..1541
                     /locus_tag="ACHGNW_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6926412.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHGNW_002139"
     CDS             1669..3045
                     /locus_tag="ACHGNW_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6926413.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHGNW_002140"
     CDS             3446..5026
                     /locus_tag="ACHGNW_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN6926414.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHGNW_002141"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHGNW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHGNW_002142"
     CDS             complement(5652..5972)
                     /locus_tag="ACHGNW_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN6926415.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHGNW_002143"
     CDS             complement(6120..8354)
                     /locus_tag="ACHGNW_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926416.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHGNW_002144"
     CDS             8833..9099
                     /locus_tag="ACHGNW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN6926417.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHGNW_002145"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHGNW_002145"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN6926418.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAZBTH010000087        10724 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000087 DAZBTH010000000
VERSION     DAZBTH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.089"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACHGNW_002146"
     CDS             complement(241..1089)
                     /locus_tag="ACHGNW_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6926419.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACHGNW_002147"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACHGNW_002147"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN6926420.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACHGNW_002148"
     CDS             2157..3158
                     /locus_tag="ACHGNW_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN6926421.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACHGNW_002149"
     CDS             3155..3439
                     /locus_tag="ACHGNW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN6926422.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACHGNW_002150"
     CDS             3472..4269
                     /locus_tag="ACHGNW_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6926423.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACHGNW_002151"
     CDS             4283..5116
                     /locus_tag="ACHGNW_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6926424.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACHGNW_002152"
     CDS             5134..5991
                     /locus_tag="ACHGNW_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6926425.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACHGNW_002153"
     CDS             6013..6522
                     /locus_tag="ACHGNW_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6926426.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACHGNW_002154"
     CDS             6559..6960
                     /locus_tag="ACHGNW_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926427.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACHGNW_002155"
     CDS             7072..7425
                     /locus_tag="ACHGNW_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926428.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACHGNW_002156"
     CDS             7459..7902
                     /locus_tag="ACHGNW_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6926429.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002157"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002157"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6926430.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACHGNW_002158"
     CDS             8997..9710
                     /locus_tag="ACHGNW_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6926431.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACHGNW_002159"
     CDS             complement(9917..10066)
                     /locus_tag="ACHGNW_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926432.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACHGNW_002160"
     CDS             10462..10662
                     /locus_tag="ACHGNW_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6926433.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DAZBTH010000088        10657 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000088 DAZBTH010000000
VERSION     DAZBTH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.090"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHGNW_002161"
     CDS             33..656
                     /locus_tag="ACHGNW_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926434.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGNW_002162"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGNW_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6926435.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHGNW_002163"
     CDS             2152..3033
                     /locus_tag="ACHGNW_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926436.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHGNW_002164"
     CDS             3036..3806
                     /locus_tag="ACHGNW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926437.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGNW_002165"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGNW_002165"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN6926438.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHGNW_002166"
     CDS             5117..6280
                     /locus_tag="ACHGNW_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6926439.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHGNW_002167"
     CDS             6397..6807
                     /locus_tag="ACHGNW_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6926440.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHGNW_002168"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHGNW_002168"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6926441.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHGNW_002169"
     CDS             9380..9664
                     /locus_tag="ACHGNW_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926442.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHGNW_002170"
     CDS             9824..10060
                     /locus_tag="ACHGNW_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6926443.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHGNW_002171"
     CDS             10057..10455
                     /locus_tag="ACHGNW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6926444.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3724 a         1773 c         2253 g         2907 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttata
//
LOCUS       DAZBTH010000089        10546 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000089 DAZBTH010000000
VERSION     DAZBTH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.091"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1883)
                     /locus_tag="ACHGNW_002172"
     CDS             complement(117..1883)
                     /locus_tag="ACHGNW_002172"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926445.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1843..3596)
                     /locus_tag="ACHGNW_002173"
                     /pseudo
     CDS             complement(1843..3596)
                     /locus_tag="ACHGNW_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3706..4275)
                     /locus_tag="ACHGNW_002174"
     CDS             complement(3706..4275)
                     /locus_tag="ACHGNW_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN6926446.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4293..5708)
                     /locus_tag="ACHGNW_002175"
     CDS             complement(4293..5708)
                     /locus_tag="ACHGNW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926447.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5719..6387)
                     /locus_tag="ACHGNW_002176"
     CDS             complement(5719..6387)
                     /locus_tag="ACHGNW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6926448.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6517..7101)
                     /locus_tag="ACHGNW_002177"
     CDS             complement(6517..7101)
                     /locus_tag="ACHGNW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6926449.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7432..7665)
                     /locus_tag="ACHGNW_002178"
     CDS             complement(7432..7665)
                     /locus_tag="ACHGNW_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6926450.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7680..8630)
                     /locus_tag="ACHGNW_002179"
     CDS             complement(7680..8630)
                     /locus_tag="ACHGNW_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN6926451.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8627..9469)
                     /locus_tag="ACHGNW_002180"
     CDS             complement(8627..9469)
                     /locus_tag="ACHGNW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN6926452.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9471..10190)
                     /locus_tag="ACHGNW_002181"
     CDS             complement(9471..10190)
                     /locus_tag="ACHGNW_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6926453.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         3566 a         1924 c         1368 g         3688 t
ORIGIN      
        1 atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt
       61 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
      121 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
      181 tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt
      241 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
      301 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
      361 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
      421 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
      481 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
      541 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
      601 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
      661 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
      721 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
      781 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
      841 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
      901 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
      961 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
     1021 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
     1081 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
     1141 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
     1201 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
     1261 tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt
     1321 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
     1381 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
     1441 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
     1501 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
     1561 gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat
     1621 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg
     1681 gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt
     1741 ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta
     1801 gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt
     1861 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
     1921 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
     1981 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
     2041 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
     2101 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
     2161 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
     2221 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
     2281 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
     2341 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
     2401 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
     2461 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
     2521 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
     2581 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
     2641 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
     2701 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
     2761 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
     2821 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
     2881 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
     2941 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata
     3001 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
     3061 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
     3121 tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
     3181 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
     3241 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
     3301 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
     3361 aaataagcca gaaaaaatat aaaataaagc tctaaagtta accaagcaac agtttgaacc
     3421 gcataactct tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa
     3481 atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac
     3541 catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt
     3601 tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat
     3661 agcgtaaatt agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt
     3721 ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac
     3781 tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga
     3841 ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca
     3901 ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg
     3961 atcatatcca aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat
     4021 aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta
     4081 aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata tgattccaga
     4141 taataatcca aaaatcccgg gggatatgct gtgaccaaac gccccaccaa tactactaac
     4201 cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa taaaaatata
     4261 atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt
     4321 tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc accatttttt
     4381 aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt agctataact
     4441 actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat attaatataa
     4501 tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa tgaagctata
     4561 gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg atctttcatt
     4621 ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac tatttcattc
     4681 cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat ttcttgcatt
     4741 actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt ggtagggcga
     4801 ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt ccctaccaaa
     4861 ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt ttcataagta
     4921 atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac ttcaatcgaa
     4981 ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa caactgaata
     5041 gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa ttctttcaaa
     5101 tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa atcacaaagt
     5161 aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc atccaatata
     5221 attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg ttcaccactt
     5281 gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc agctaattca
     5341 ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga aaatactaac
     5401 tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac gtagccaata
     5461 ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa taaaatttcc
     5521 ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt taatagtgtg
     5581 gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt tattgaaaaa
     5641 ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac attgttaaat
     5701 tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa cggaaaacag
     5761 gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt tcacaggaga
     5821 ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag ttgcacgtat
     5881 aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag acaagcgaat
     5941 gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag tcggaaagaa
     6001 tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt tttctaaaga
     6061 ggataataat tcattgctag tatagacact aaccaacaac catgagatca ttacgaccgg
     6121 caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa actctatctt
     6181 gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga tgaaataaat
     6241 acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga aacaccccac
     6301 ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat ttaaagtaat
     6361 caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga tcgcactgtt
     6421 atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta tcaaaaagca
     6481 taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt caatccaccc
     6541 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
     6601 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
     6661 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
     6721 gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
     6781 agctgattgg aataaaatca aatcgaatat acgtttattc tcaaaaatat aattaataaa
     6841 tggatctaat tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt
     6901 taagaggtat ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg
     6961 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
     7021 tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa
     7081 atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat
     7141 gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc
     7201 aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga
     7261 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
     7321 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
     7381 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
     7441 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
     7501 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
     7561 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
     7621 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
     7681 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
     7741 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
     7801 ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag
     7861 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
     7921 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
     7981 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
     8041 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
     8101 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
     8161 taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc
     8221 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
     8281 tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta
     8341 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
     8401 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
     8461 gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg
     8521 ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga
     8581 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct
     8641 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
     8701 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc
     8761 caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat
     8821 ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc
     8881 ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat agataatgat
     8941 aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata
     9001 attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc
     9061 acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg
     9121 agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac
     9181 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
     9241 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
     9301 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
     9361 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
     9421 tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattccccc
     9481 tctccaataa atatagtatc tccataggct ttttttaaat tttcttcatt aaatacagat
     9541 tctacacttc cgtgagcaat tagatttcga ttcaaaagaa taatattatc aaaatacttc
     9601 tttaccttgc ttaaatcatg atgcacaatt agcactgttt ttccctgatg tttcaatttt
     9661 tttaacgtat ccataattat tcgttcacta actaagtcta tccctacaaa tggttcatct
     9721 aaaaatatga aatccgcatt ttgagcaaga catcttgcta aaagtacacg ttgaaattgt
     9781 cctccagata attctcctat ttgatgatta gaatattttc ccatatcgac cttttccaaa
     9841 gcctttgaaa ctctttgcca ttctgcattt ttaattcttt gaaaaacttt cattccggca
     9901 tatgttccca tagatacaca ttcttttact tttattggaa aagtaaaatc aatatcagct
     9961 ttttgctcta catatgcaat cttcaccagt tttttattga tatcttcatt attaatggag
    10021 acctctccct gatgagggat aatatttaac attgctttaa gcaatgtgga ttttccagca
    10081 ccatttggac caataatacc tgttaaagca ccttcttgta tttttaaaga aatctcgttc
    10141 aatgctaaaa aatcgttata acaaacatta agctttttca cttctaacat aactttctcc
    10201 ttctaaatga taatgattat cacgatcata tggtatacaa aaattatatt ttagtcaacc
    10261 ctaaatttta tagtttagat ttttttctgt tgctgaagat tagaatgaca aaactatttg
    10321 aaactacgcg tatagtaagt agctttcaaa atatataaaa aaggagcgtt ctcaaatgag
    10381 aacatgctcc taaaactact ttattggcgt atggaaaaat tatacttgaa gtggatagcg
    10441 ggttgaataa gttgataagc atcaaaacat ttataaaaat tattcaagac tatgtctgtt
    10501 ccatatacaa atatgaaaat cagtaaactc catttaactt ttttcg
//
LOCUS       DAZBTH010000090        10335 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000090 DAZBTH010000000
VERSION     DAZBTH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.092"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..335
                     /locus_tag="ACHGNW_002182"
     CDS             186..335
                     /locus_tag="ACHGNW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN6926454.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            346..1860
                     /gene="dltA"
                     /locus_tag="ACHGNW_002183"
     CDS             346..1860
                     /gene="dltA"
                     /locus_tag="ACHGNW_002183"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN6926455.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1857..3062
                     /gene="dltB"
                     /locus_tag="ACHGNW_002184"
     CDS             1857..3062
                     /gene="dltB"
                     /locus_tag="ACHGNW_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN6926456.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3107..3340
                     /gene="dltC"
                     /locus_tag="ACHGNW_002185"
     CDS             3107..3340
                     /gene="dltC"
                     /locus_tag="ACHGNW_002185"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN6926457.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3343..4611
                     /gene="dltD"
                     /locus_tag="ACHGNW_002186"
     CDS             3343..4611
                     /gene="dltD"
                     /locus_tag="ACHGNW_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN6926458.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4752..5834
                     /locus_tag="ACHGNW_002187"
     CDS             4752..5834
                     /locus_tag="ACHGNW_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN6926459.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5834..6637
                     /locus_tag="ACHGNW_002188"
     CDS             5834..6637
                     /locus_tag="ACHGNW_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6926460.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6707..7447
                     /locus_tag="ACHGNW_002189"
     CDS             6707..7447
                     /locus_tag="ACHGNW_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6926461.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7458..8462
                     /locus_tag="ACHGNW_002190"
     CDS             7458..8462
                     /locus_tag="ACHGNW_002190"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN6926462.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8507..10333)
                     /locus_tag="ACHGNW_002191"
     CDS             complement(8507..10333)
                     /locus_tag="ACHGNW_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926463.1"
                     /translation="MYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQM
                     KEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQ
                     WITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPK
                     VYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCF
                     ALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFC
                     TFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACT
                     PWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQ
                     VDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKIL
                     EEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLI
                     HALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQ
                     ERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3323 a         1703 c         2087 g         3222 t
ORIGIN      
        1 tattttttat attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt
       61 gctattttta taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta
      121 gacacatgtt tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga
      181 atccaatgaa caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc
      241 gaacaatttt ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata
      301 ttcaaggagg aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg
      361 attattgaag caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg
      421 gatgagacgc atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta
      481 gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg
      541 attgtctcat ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat
      601 acacccaagg agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt
      661 atcggggact ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa
      721 gaaatcaaga agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat
      781 ttttatatta tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat
      841 gataatctag tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg
      901 catttcttag cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca
      961 ttagtttctg gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa
     1021 ttattcgcga cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg
     1081 gatatttgct tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt
     1141 ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca
     1201 aatgcacgga tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt
     1261 cagattgacc agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa
     1321 gacacgaaag tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa
     1381 atcattattg caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct
     1441 gctgcgttct tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta
     1501 gatgaatatg ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga
     1561 taccggattg aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa
     1621 gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta
     1681 ttagaaaaca aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta
     1741 ctagaagttg taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct
     1801 cagacagtta atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga
     1861 tgttacctca tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc
     1921 ttgtaccgat cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag
     1981 cagtgacact tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg
     2041 cgctgattgg gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc
     2101 aaaggaaaaa tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt
     2161 ttttaacgaa agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag
     2221 gaatctctta tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga
     2281 tcaaaacata taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt
     2341 ctggaccaat cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg
     2401 ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata
     2461 aattcattat cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct
     2521 tacagcatgg tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt
     2581 tgttttttga ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat
     2641 atcagacacc aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga
     2701 atcgttggca tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt
     2761 tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt
     2821 ttgctttgtt tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg
     2881 gactgtatca tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc
     2941 ataaagagca gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc
     3001 aagcagtatg ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct
     3061 aacgaatagt gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat
     3121 tttatcaatc ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga
     3181 tctatttgat gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga
     3241 cggagagctg gatatccaag tccctgtatc tgaattcgag cgagaggatt ggagcacacc
     3301 gaataaaatc attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt
     3361 gctattttcg gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc
     3421 tatcaagtag atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg
     3481 aacgtgttga aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct
     3541 ttttttggct caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa
     3601 aaataccagc gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc
     3661 caatatatga tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt
     3721 atttctcctc agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat
     3781 tctgaacttc aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc
     3841 tatttagcta gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg
     3901 attctgcaaa cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc
     3961 agccaatgga acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat
     4021 cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa
     4081 ttatccattt tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg
     4141 aaaaacgatt tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa
     4201 aaaaattggg attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa
     4261 ttagcaaaaa accataacga agtattgttc atcatcccac cggttaacga gaaatggagc
     4321 gattataccg gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa
     4381 ttaaatagcc aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat
     4441 tttatggaag acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc
     4501 agaccgtttt tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt
     4561 tttagcaaat cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag
     4621 gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac
     4681 caagtttgcc tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg
     4741 ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag
     4801 gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg
     4861 ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag
     4921 gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa
     4981 ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg
     5041 ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca
     5101 aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt
     5161 tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag
     5221 atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa
     5281 aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta
     5341 aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc
     5401 gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg
     5461 gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag
     5521 gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg
     5581 ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag
     5641 gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg
     5701 ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata
     5761 cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc
     5821 ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt
     5881 ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac
     5941 gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca
     6001 gcacttattg agttttctag tcgataattt acttttattc gcattcaact atgcattgtt
     6061 ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt
     6121 tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa
     6181 ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta
     6241 tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct
     6301 tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag
     6361 tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc
     6421 ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat
     6481 tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg
     6541 tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg
     6601 gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa
     6661 aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt
     6721 tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca
     6781 atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga
     6841 acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat
     6901 tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac
     6961 attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga
     7021 aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct
     7081 gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga
     7141 aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga
     7201 ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca
     7261 tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa
     7321 gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc
     7381 gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa
     7441 catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt
     7501 tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt
     7561 atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc
     7621 gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg
     7681 tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat
     7741 tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg
     7801 ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca
     7861 acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg
     7921 atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa
     7981 tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa
     8041 aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag
     8101 tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct
     8161 ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct
     8221 ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa
     8281 tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg
     8341 aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa
     8401 ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt
     8461 gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat acaggattcg
     8521 ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta
     8581 gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat
     8641 catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt
     8701 cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca
     8761 gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt
     8821 gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct
     8881 gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt
     8941 ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat
     9001 agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga
     9061 caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt
     9121 gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat
     9181 ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa
     9241 tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc
     9301 aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct
     9361 tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat
     9421 tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc
     9481 cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca
     9541 ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata
     9601 aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt
     9661 caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt
     9721 gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta
     9781 tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg
     9841 ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa
     9901 gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg
     9961 gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag
    10021 taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg
    10081 tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc
    10141 tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt
    10201 tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta
    10261 aaaataacaa tgataatata atgattatgc tccactttat acgtaaataa aaagattctt
    10321 ttttattata tatgt
//
LOCUS       DAZBTH010000091        10192 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000091 DAZBTH010000000
VERSION     DAZBTH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.093"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..625
                     /locus_tag="ACHGNW_002192"
     CDS             119..625
                     /locus_tag="ACHGNW_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6926464.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ACHGNW_002193"
     CDS             642..1541
                     /locus_tag="ACHGNW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN6926465.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ACHGNW_002194"
     CDS             1628..2512
                     /locus_tag="ACHGNW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6926466.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ACHGNW_002195"
     tRNA            complement(2704..2775)
                     /locus_tag="ACHGNW_002195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHGNW_002196"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHGNW_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN6926467.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ACHGNW_002197"
     CDS             4318..5409
                     /locus_tag="ACHGNW_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN6926468.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ACHGNW_002198"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ACHGNW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6926469.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /gene="pgk"
                     /locus_tag="ACHGNW_002199"
     CDS             6561..7751
                     /gene="pgk"
                     /locus_tag="ACHGNW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN6926470.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHGNW_002200"
     CDS             7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHGNW_002200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN6926471.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ACHGNW_002201"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ACHGNW_002201"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN6926472.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DAZBTH010000092        10182 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000092 DAZBTH010000000
VERSION     DAZBTH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.094"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACHGNW_002202"
     CDS             102..2543
                     /locus_tag="ACHGNW_002202"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN6926473.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACHGNW_002203"
     CDS             complement(2873..3577)
                     /locus_tag="ACHGNW_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926474.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ACHGNW_002204"
     CDS             complement(3764..4009)
                     /locus_tag="ACHGNW_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926475.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ACHGNW_002205"
     CDS             complement(4026..4877)
                     /locus_tag="ACHGNW_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6926476.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ACHGNW_002206"
     CDS             5043..6047
                     /locus_tag="ACHGNW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN6926477.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6830..7387)
                     /locus_tag="ACHGNW_002207"
     CDS             complement(6830..7387)
                     /locus_tag="ACHGNW_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926478.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(7566..8177)
                     /locus_tag="ACHGNW_002208"
     CDS             complement(7566..8177)
                     /locus_tag="ACHGNW_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926479.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(8290..8547)
                     /locus_tag="ACHGNW_002209"
     CDS             complement(8290..8547)
                     /locus_tag="ACHGNW_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926480.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(9144..9422)
                     /locus_tag="ACHGNW_002210"
     CDS             complement(9144..9422)
                     /locus_tag="ACHGNW_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926481.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(9456..9707)
                     /locus_tag="ACHGNW_002211"
     CDS             complement(9456..9707)
                     /locus_tag="ACHGNW_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926482.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         3199 a         1892 c         1840 g         3251 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctcctta ttccgaataa atggtgtcca aaagcttgca tctgccagat
     6301 tatctcattt cttatgacaa gctaagcaaa ttatctccac caaataattt attcatattt
     6361 ttgacttgcg catactaact aattcttaaa ttttcataca aaatttctca atttccctta
     6421 acgagtaata tttatgtacg gtgattctca ctagtatccc aatcactttt taagttatcc
     6481 cgatttcaat tctacttcaa aaatagttgc ttttatgtat gctgttagtt gattagatgg
     6541 tcatttgttg agctaggtcg tttattttaa acaagttgtt tctttagctt atacctatct
     6601 cgtctaccaa acgcaagatg gcaaacacaa atgcatacgc atctgtattt gccactttga
     6661 ttcgtataaa gctaacaact attaactcct ctcagctaat agttgctctc ttttttattc
     6721 agattcattt ttgctgatca cagcctagta tttcaatggt tttgaacact aaaccaaatt
     6781 gttccattac aacattcact ggctgatgaa atggattagt agatttcgat tattcaaagc
     6841 gattttctat tggtggatcg tgttcccaac taataagacg gattccaaac ctatcgtaca
     6901 gatcttctaa accacttata aattttgcac ctatttcagg tgttttattg cgtggatatt
     6961 ctattgttac gccattaaat ggataccaat taggattagg ctctcttttt ttctgtacat
     7021 ctcccataga atagttagca ctttctggga cataatatat tcttcgacca tcctctaatt
     7081 catctaataa gatatttcgt gatcgtctat cacttgctaa atgtagacgc cacgtgcgat
     7141 accatgccat gctacctgca tctgcagaaa atccatcaaa taatgcaaca attgtatctt
     7201 ctatcacaat cctttcccca tctacttcat attctaatct aaaatcaaac tcccctcggg
     7261 taatttctgg tcttggtggc ttaggaccca atattattct acttaaaacc gcattcgttc
     7321 ccagatgaaa cacagctata acaacaataa tgattaatat tttgtttcta tttgataaat
     7381 ttctcattat tcgcacatca cctctacatt ctcacatatt agttcgtaat actcctttaa
     7441 tatcaaaata tctactggtc gtaataatat tcgctatgaa ttcctactac tcgtataaga
     7501 aaggccaaat catcagcttt tacttgctga tgatttggct tgtttctatt tatcgtaatt
     7561 gttttctaat caccaaaatt gttaacaatc ggttcagata tttcccagtt gattaaggta
     7621 attccgtatt gtgtcaaaac ttcttgcgct tctccgaaat taagtacagt tcttatgtat
     7681 ccactttctc ctaatataaa gtctacctct cttttaaaac cttcctcgag atttctaagg
     7741 gctatatgtg gttttaatga cattgattgt acattttccc ccatataata ttgcgcactt
     7801 cctcgactaa aatagattcc tagatcgtcc ccaaaaggta gatcttctct accactagct
     7861 aatctacttc tccatcttcg ttctcttttc atagaacctg cgctgaactc acacccgtta
     7921 aactcagcta tgacagtatc ttcaattatg ttaatctgtc catctagttc gtattcaatc
     7981 ctaaatggaa attctccata ggtgatttcc ggttcgggta ttttaggtga gattaatgca
     8041 aagaaaggaa atattaagct tggcaacatc aaaaaaactc ctcctatgat cacaattccc
     8101 gatgtaatcc aaaatataat ttgtacaatt ctcattgatc cattgtattc cattttattc
     8161 cccctattac tattcatatc cgcctatcta tatcgatctt ccaatgcaga gataattgct
     8221 tccatacctt gattttttac tgagttacct atgcgagata tcattctcca ctaaggtgca
     8281 tcatattgtc tatctaacat gattaattct ccaataggtg tatactcctc caaaaacaac
     8341 atttaatgca tctccctcga caacataagc taccatgttt gctgtatatg tgtttgagtc
     8401 taattcataa gcagataaat ttactgtcgc tggattaaat ataatcgcat tcgtatttat
     8461 agctactgaa tttgctgctt cagcaccacc ttttgaatgt ccgatcattg tgatttcaat
     8521 agtaggattt acgtggacag ccctcaccgt ttaccctgac cattatagaa atcgcttact
     8581 tttttgaact acgcaacaaa ggatacaatg ctcctaactt cactgtctcc atttttgtcc
     8641 actaaggaat ataaaaaata ggcattaatt catttaattc ggtcacaaca cgttaccttt
     8701 taaatcatta tactgtatct catacattat tatcaataaa ctattataaa aatgcaaaaa
     8761 atgctttatc aatctatttt gtaattggtc ataatatcat tgaaatcata ccctgtatcg
     8821 atttttccta tgaatctcgt ctatttgtga ttgacgaaaa agaggcaaga cagacactgg
     8881 aatggtaatt ttacccacta ttttgctcca actatttgta atcgtaccca cttttgaaat
     8941 accgagatga tcattttaat tgatatgctg attcccaagc aaaaccccaa gaaattaaat
     9001 gctctatcgc atctagcttc ttgggttttg cttatataaa ttccaacatt taaatttttt
     9061 atgctgttcg gtctcagaaa tgccgttttt gcttatatca caatatcaag atgtcaaatt
     9121 attcgtgttg ccgatgactt tgattaattt ttttcaaatt gtttattaag actaatgctg
     9181 tctttttgca gataccaatg ttcgaacttt ttaacattat catatgatcc tgcaagtttt
     9241 aacttcttac cattagatgt tatcaaagtt ataattaacc ttgccttttt cccattagga
     9301 tcttttattt tatgaatttt ctgcaggtaa tctctcctaa tggcagctat ttttacctca
     9361 tcagtgctga taaaccattg atcatccaca tacatactgc ctgttatgtt gtgtttttcc
     9421 atttcttccg agaacgaaaa gtttaaggct cgttcttact tccaaagttg aatgagtcca
     9481 tggattggac ttagtgtgag aattgagaac aacaataaga atccatatcc tcccgcaaac
     9541 atccctaagg ctatactgtc tctccatcca taagtaggat tagaagagaa aagagtcacc
     9601 gtttgtaata atatgaaaag gaataatcca gcacaaacac caaagataac aactccaaac
     9661 attgaatttt tatacaatga ccatttattt ataggctttt ctttcatttt acttccccct
     9721 actgttctgt tcacacggaa tgaattcaaa gttcataagc cctcctcttt gaacatatga
     9781 atacgacttg ctaactatac tttaaaaaag cttgttttaa gcggttatag gcgagttgta
     9841 atctctattc atctcattag caaataactt gccgaaaagt ctactgaaat taaagcagat
     9901 agtggaagaa ttatttagcc aggatagaca ctatttgtga aacacatgtc tttacttttg
     9961 aaattctatg atgaaaagcc ttggaataca gtagctctta attggtaata ctttttccat
    10021 tataaataac ctcttttcat tttatattta tcccctctaa aaataatatc taatctaata
    10081 ttttaaactt atatttccct cgctgtgcta atagagcatt actaaataaa tagaaactag
    10141 aaaaaagcat tacgcctgcc agaataaacc aattatttgt at
//
LOCUS       DAZBTH010000093        10142 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000093 DAZBTH010000000
VERSION     DAZBTH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.095"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHGNW_002212"
     CDS             278..787
                     /locus_tag="ACHGNW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6926483.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHGNW_002213"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHGNW_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6926484.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHGNW_002214"
     CDS             2322..3302
                     /locus_tag="ACHGNW_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6926485.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHGNW_002215"
     CDS             3424..4842
                     /locus_tag="ACHGNW_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN6926486.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHGNW_002216"
     CDS             4913..6382
                     /locus_tag="ACHGNW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6926487.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHGNW_002217"
     CDS             6392..6859
                     /locus_tag="ACHGNW_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6926488.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHGNW_002218"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHGNW_002218"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6926489.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>10142
                     /locus_tag="ACHGNW_002219"
     CDS             7848..>10142
                     /locus_tag="ACHGNW_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6926490.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYD"
BASE COUNT         3523 a         1464 c         2015 g         3140 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 at
//
LOCUS       DAZBTH010000094        10086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000094 DAZBTH010000000
VERSION     DAZBTH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.096"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACHGNW_002220"
     CDS             complement(247..759)
                     /locus_tag="ACHGNW_002220"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN6926491.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHGNW_002221"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHGNW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN6926492.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACHGNW_002222"
     CDS             complement(3212..3655)
                     /locus_tag="ACHGNW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN6926493.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACHGNW_002223"
     CDS             complement(3656..4444)
                     /locus_tag="ACHGNW_002223"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6926494.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHGNW_002224"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHGNW_002224"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN6926495.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACHGNW_002225"
     CDS             complement(5559..6146)
                     /locus_tag="ACHGNW_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN6926496.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHGNW_002226"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHGNW_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN6926497.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACHGNW_002227"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACHGNW_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6926498.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACHGNW_002228"
     CDS             complement(9063..9998)
                     /locus_tag="ACHGNW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926499.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAZBTH010000095        10084 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000095 DAZBTH010000000
VERSION     DAZBTH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.097"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHGNW_002229"
     CDS             162..392
                     /locus_tag="ACHGNW_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN6926500.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHGNW_002230"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHGNW_002230"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6926501.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHGNW_002231"
     CDS             1803..1979
                     /locus_tag="ACHGNW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6926502.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHGNW_002232"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHGNW_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN6926503.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACHGNW_002233"
     CDS             2474..2941
                     /locus_tag="ACHGNW_002233"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926504.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHGNW_002234"
     CDS             3026..3910
                     /locus_tag="ACHGNW_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6926505.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHGNW_002235"
     CDS             4024..4260
                     /locus_tag="ACHGNW_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN6926506.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHGNW_002236"
     tRNA            4348..4428
                     /locus_tag="ACHGNW_002236"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHGNW_002237"
     tRNA            4435..4506
                     /locus_tag="ACHGNW_002237"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACHGNW_002238"
     CDS             4631..4801
                     /locus_tag="ACHGNW_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926507.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACHGNW_002239"
     CDS             5021..5728
                     /locus_tag="ACHGNW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6926508.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACHGNW_002240"
     CDS             complement(5788..6432)
                     /locus_tag="ACHGNW_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN6926509.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACHGNW_002241"
     CDS             6559..6846
                     /locus_tag="ACHGNW_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926510.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACHGNW_002242"
     CDS             6954..7274
                     /locus_tag="ACHGNW_002242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6926511.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACHGNW_002243"
     CDS             7355..8668
                     /locus_tag="ACHGNW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6926512.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACHGNW_002244"
     CDS             8665..9384
                     /locus_tag="ACHGNW_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN6926513.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACHGNW_002245"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACHGNW_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN6926514.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAZBTH010000096        10049 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000096 DAZBTH010000000
VERSION     DAZBTH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.098"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..384
                     /locus_tag="ACHGNW_002246"
     CDS             <1..384
                     /locus_tag="ACHGNW_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN6926515.1"
                     /translation="ATSEKGFNFDWQTTLFVSVIYFALLLGIVIAYFLLKLLKKIEDN
                     QAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDAPGLMLVGIAVVFIPFAV
                     YTLTSVLEKLLEHAILLKTDHDLTV"
     gene            393..620
                     /locus_tag="ACHGNW_002247"
     CDS             393..620
                     /locus_tag="ACHGNW_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926516.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1256..1627
                     /locus_tag="ACHGNW_002248"
     CDS             1256..1627
                     /locus_tag="ACHGNW_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926517.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1883..2125)
                     /locus_tag="ACHGNW_002249"
     CDS             complement(1883..2125)
                     /locus_tag="ACHGNW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6926518.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2157..3059)
                     /locus_tag="ACHGNW_002250"
     CDS             complement(2157..3059)
                     /locus_tag="ACHGNW_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6926519.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3072..3260)
                     /locus_tag="ACHGNW_002251"
     CDS             complement(3072..3260)
                     /locus_tag="ACHGNW_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6926520.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3274..3837)
                     /gene="amaP"
                     /locus_tag="ACHGNW_002252"
     CDS             complement(3274..3837)
                     /gene="amaP"
                     /locus_tag="ACHGNW_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6926521.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3875..4768)
                     /locus_tag="ACHGNW_002253"
     CDS             complement(3875..4768)
                     /locus_tag="ACHGNW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6926522.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4846..5769)
                     /locus_tag="ACHGNW_002254"
     CDS             complement(4846..5769)
                     /locus_tag="ACHGNW_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6926523.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5818..6168)
                     /locus_tag="ACHGNW_002255"
     CDS             complement(5818..6168)
                     /locus_tag="ACHGNW_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6926524.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6201..7103)
                     /locus_tag="ACHGNW_002256"
     CDS             complement(6201..7103)
                     /locus_tag="ACHGNW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6926525.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7096..7953)
                     /locus_tag="ACHGNW_002257"
     CDS             complement(7096..7953)
                     /locus_tag="ACHGNW_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6926526.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8287..9096
                     /locus_tag="ACHGNW_002258"
     CDS             8287..9096
                     /locus_tag="ACHGNW_002258"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6926527.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9136..9633
                     /locus_tag="ACHGNW_002259"
     CDS             9136..9633
                     /locus_tag="ACHGNW_002259"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN6926528.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2896 a         1914 c         1732 g         3507 t
ORIGIN      
        1 gctacaagcg aaaaaggatt caattttgat tggcagacca cattatttgt ttctgtcatt
       61 tactttgccc ttctcctagg gatcgttatt gcttatttct tattgaaact attgaaaaaa
      121 atcgaagata accaagcttt ttccactcaa agcttaaaca tactaaaaaa aatcgaacga
      181 accattttgt ttattggttt cgcctttttt ggcttacttc ctaaatttat gaaagtagcg
      241 gatttagacg atgctccagg tttgatgttg gtaggaatcg ctgtcgtatt cattcctttt
      301 gcagtataca cattaacaag cgtgctggaa aaactgcttg aacatgcgat tttacttaag
      361 acagatcatg atttgacagt gtgaggtgcg acatggcaat tatcgttaat ttagatgtag
      421 tattagctca aaaaaaaatg agtgtgactg aattgagtga gaaagtgggc attacgatgg
      481 caaacatctc tattctaaaa aatggaaaag caaaagccat ccgtttttcc actctcgaaa
      541 aaatctgcca agttttggag tgtcaaccag gtgatttgtt gacctataaa aatgactcga
      601 atactatacc agaagaataa aataaagtag aacaaacagt aaataaaaaa taatctgaat
      661 gatagatgtg tgagactttt agaagttatt tacgtctatt attcagattt tttatttcga
      721 ttgaataccg ttttatctat aaataagata tgtactgact catacaaagg gggccttttc
      781 ttctccatgt actatcactt ttcctgatac aactggtttc ctgttacgtt gtagttggat
      841 ctgcttttag tataatactt tattcattcc accagactga tagcagtttg agcagtctaa
      901 ataatcatgt agtattacag gccattctgt ttcatacctt gaagtgctta aaacttcagc
      961 taatggccca ttgtgttaac tcttactcca ttctttttta gtaagctccc atatatactg
     1021 taaagccttc tatatattat gtcatgttgc tttttaacat ttactatgta gttttgataa
     1081 aatgatttga ataacagtga tggacaattc ttttttattt ttaacattat tttgataata
     1141 atgattattt atttaaaaat acattagata acatttgtta cgacacgaaa aagcttcaca
     1201 aacaaatatc gttattttaa aatgagggcg aactcacaca attaggagga atagtatgcc
     1261 atttattgtt tacttttttc tttcattact cgtgatctat attccattac caacattttt
     1321 cctgctcgtc aatcgtctag cagagcaaca tgatacgatt gctgctctta cgaaactttg
     1381 tttacttcta tttgtttgtc tagattactt tacgagctat tattggctaa aacagacaag
     1441 agtaaaaaag agatattatt tcttccttgt ctttttcaat ctctttgagt ttctgatcca
     1501 ttgcttttta cttcatcagt atcatacgat ccatttgtgg atcatctggg ttttcaaagt
     1561 ggtcgtatta ttttgtatgt tcacctttcc tatctcaaga aaatttaggg aggcattgtt
     1621 ttattaaaga agcttctctg tatcaataaa gacacattta aggaaaaact tttaaaaatc
     1681 taattgggaa tttgcccgca taaataagag gctgtgacaa aagtcttgtc acagcctctt
     1741 attccgtata aacagtgttc aaaagcttgc atcttcgata atatgcccaa ccaaatgaaa
     1801 aatatgagaa gctatttttg tttggctgtt cttattactt gatgaagaac agctttttca
     1861 cgacctcttt ttaaaagtct ttttacagtt ttcgagcaaa gaatgatacg attagtacta
     1921 aaatgatggc accaatcaat gatggcacga ttgccattcc tgcaatactt ggtccccaag
     1981 aacctaataa tgcttgacca agtgctgatc caactaaacc tgctacgaca tttgcaataa
     2041 tccccatcga agaaccttta ttcgtgattg ctcctgcaat tgctccgata attcctccga
     2101 cgataagtga ccaaataaat cccatgtttc gttcctccta atgttttact tccttattac
     2161 tgaacccgtg gttctgtatt ttcttttact ttttgtgcgc cgcttccagc tgctgatttt
     2221 actttatctg tttgattcga agcaaattca ccggtttttt tcgctgcgtc agttactttg
     2281 tcttgcaccg tttcttgatc tttttcaaat tcttcttttg ttttgatgtc cactacgtta
     2341 gcgttgactt cgatcacttc taaatgtgtc atatttttta cttctttgcc gattacttct
     2401 tgcatttctt caaagatttt ttcgatatct ttgccatatt ccacaatgac atccatatca
     2461 actgctactt gttttttacc gacttctgtt tcgatcccag ccgttacatt atctgtattc
     2521 acaagttttc ctgccacatt agagaagaaa ccaccgtttg cagataataa accgtctact
     2581 tgttcaattg cgattccgat gattttttgc acaactttat cttcataatt cagctcaccg
     2641 cgcattgaat cttcctgttc agaatcatgc tttccttggt tatttgtatg atcagaacca
     2701 agttttcctt gtccgtttgt gtggtcagaa ctaagttttt cttgtgcttt cgcatggtca
     2761 gaaggatgtt tttgctcttc cacaggttta gaaggatcga ttgcatcatg ttgttctgga
     2821 actttagatt ctggagttcc tggatgatga ggtgcatgct tttctgcatc atgtttcttc
     2881 tcttctactc cttgttgaac gttagggact actggatctg gatgtgcaga tactggtgct
     2941 gttttgttct tttcatcgtt cacaccattt gattggtttg gtgtcgttgg gtctggatgt
     3001 gcagacactg gtttttggtt gttcatttta ttgttattat ttttgttatt gttattcatg
     3061 gatgtttcct cctaagattt aaaatagtga tctaagattt tcgtttgttt cagataaaaa
     3121 ccgatagcga cgccgatcgc tgccatgatc aagatcaata aggtcttgaa aaagcccacg
     3181 gtgatccata aaacggctaa tatcaatcca agtaccccaa agatgatagg gagcttgtat
     3241 gtcgaaaata gttctttcat ttttggtccc cccctattct actcgtttgc ggtcattgct
     3301 tcgtttagct ggttcttcaa agcctgtgta tttcaccgat actttcactg gctcttttgt
     3361 tcccaatatg tgttgtactt cattttcgat ttctttcatc aatgttcctg ttttaccaat
     3421 gagtgctgaa gttcgtttca actccccttt tacattgacg gcgatcttgt ttttcgttgc
     3481 tttgacattt acttttggtg aatggatgaa ttcatcttca tgcaagtgag aacgaaccat
     3541 tccttcaatt gcttttttat ccaacgtcaa ctccccatca ccatgtttca gtagaaagtt
     3601 tccgattgtt tttggataaa acaggaaaac aagtaacgtg ataattgtca aaatagcaaa
     3661 tgcaacggca agccaaaata catattgttg cacgtatgaa ttagtgatgg aaaagaatcg
     3721 aaacttctct agctgaaaag gcaatggata ggttgcactt aatagtatcg caaacaaaag
     3781 cacagatagc aaaagtaatg ttccgatcac tccgatcgtt tttacacctt tattcatttt
     3841 cgcacctcct cataggggtg aaacgatcaa cttcttataa gttgatcgct tctccaccag
     3901 ttactccgta aatttgggct gtaacataac ttgctttatt cgatgctaag aaaacataaa
     3961 ctggtgctaa ttctcctggc tgacctgcac gttctagtaa actttgctga ccaaattcag
     4021 ggatcgaatc agaaggttgt ccttcgtcta gttgtaatgg tgtccagatt ggaccaggtg
     4081 ctacaccatt tacacggatt cctttaggtg caagctgttt tgctaaaccg atcgtaaagt
     4141 tagcgattga agcttttgtt gctgcgtaat ctaataaatg ttcgcttgga ttgaatgctt
     4201 gaacagaagt tgttgttacg attgctcctc ctgctgggat atgcggaaca gcttctttca
     4261 ctaaagcaaa catagcgatg atgtttatca taaatgtgtc tttgacttgc tcaagtggta
     4321 actcagcaat agactgacaa gaaatttgct gtcctgcgtt tagtaccatc gtatctagcc
     4381 cgccaagttc actcacggct ttatctatga tttctttagg tgcttgttcg tcacgaagat
     4441 caaaaggtag taagacaatc ttacgtcctg catctttcac gtattttgct acttcctctg
     4501 catctttttc ttctcctggg aagaattgaa gagcgatatc tgccccttct ctagcaaatg
     4561 caatcgctgc tgcacgaccg atccctgaat ctccaccagt gattagtaca cggcggtttt
     4621 ctagttgatt attgccttca taggattctt ctccacaatc aggaacagga atcatattct
     4681 gctgtaaagc tggtgtatcc tgatcttgtt taggaaattt ctccgtatgg tacaagtttc
     4741 gcgggtcgat taattttggt tctgtcattg ctgtattcct cctagttttt aaattgtatt
     4801 gccaaaacaa atttctttgt ttgatacctg ttttaaatag attctttaat ttgtataatc
     4861 tttccggtat aaatggtaac ctaaaactac tgctgctatc aaagagccaa agactacgag
     4921 tataaagcct agagcagaac ttttacccgg taatcctcct gtattcattc cccaaatccc
     4981 tgcgaacaac gccggaacgg aaatgattaa tgcagctgaa tctagatatt tcatgatatg
     5041 atttagatta ttgtccatca tcccgttgaa tagatcacca atactctcta ataaatctcg
     5101 atagatctcg atcatttttt ctgtttgtcg ctgtctcaaa tggacatcat agagtagctg
     5161 ttcatcgtct aaacgtttga tgaaagtttc agaattccat aattgatcca ttgtttcccg
     5221 ttgatctctt aaggtatgat cgatatatac gatgtctctt tcaagatctg cctgtttata
     5281 cagttcttca ttttccgttg ttttacgagc tgctttatcc aaagcgtcaa ttcgcttttt
     5341 catctctctt agttctttca cgtaatgggt gtaaatatct aataatgtat acgtgatgat
     5401 cttttcaaac gagtttaaat gatttccatg ctttgtcagc agccgatcca caaattcggt
     5461 ttttctatcg atacaagtaa tcaagagatt atcggaaagt acaaaagata ttggcgttaa
     5521 ttttttttcg atacgattat ctgactgatc tagattaatc actaccaaag aaaatggatg
     5581 ctctagtttc gtctcataaa ggcgctccat ccgagaaact tcttccggat attcggttcc
     5641 tacgaaaata tcatcaggaa gctcgaattc ttcgattgct tgctcaatct cttcgggctt
     5701 tgcactatca agtacgagcc aattatattt tttttcatct gtttctatta cattcttttc
     5761 ttcatccaca aagtagtatc tcaatgtata cctccttttt ggactttctc gaaactatta
     5821 gctgaagaag cagttcttta atttgatatt ataatcgagt actgcgattt cattttctaa
     5881 tgaaaagctg tcaagctgtt tgttgaagtc catgttcaat cgtttaccga aataattttg
     5941 ctctaattta gatgtataga tgtccaataa tggatgctcg attttagtcg catattctcc
     6001 aaattccgaa acaaaaggaa tgatttgaaa aaagctgcct tttgcaaaag cacctttgtc
     6061 ccttagaaat ggatcggaat cattgataag cgcgagtgcg atttttgtat tttccccaac
     6121 cttttcaatt aacgtctcta ctgcattttt agacattgta atttccattc ctactccccc
     6181 tcttcagttg ttgtcttcta ttatttaaca actacatctg tttttggttg aacttttgca
     6241 tattttggat atttagggaa tttagctaca caacggaaag ccgtcatccc tgcatatttt
     6301 tcgtaatatt tcttcgttgg cccttcaaaa tccttgctcc acggtttcac atgtcctgaa
     6361 aaatggataa tgtaaggatt gttgcgagtt tcttcgtatt cacgttcacc tgctgctgtt
     6421 ggatgttttt ttgcttttgc catgatatag ccttgtgcat tccaacgcgg atgaagtggt
     6481 aaccaacggt catgtaagat agcatttaat gcatcttggt catggaatcg taatttttct
     6541 ggattatgtt cgatgaaatc taatactttt tgcgtaatat tttcatctaa ccattttttc
     6601 acattgatca gcatcaaacc tgagttgaaa taacgcattg atttagctga aattttcatt
     6661 ttttccagtc gttgatggaa cccagcgtct tcaacagctg ctacgattga atcaccgaag
     6721 tcaagacgcc ataatttaga gatatcttgt aaagcgatca tgtcgcaatc catatataag
     6781 atacgttcaa cttctgtgcc gcggaacaat tccggaatcg caatacgata ataagctgtt
     6841 tctggaatac gatcgctgat taccacattt gtaaagaatt ctttattgat tttcaagaat
     6901 tccacatctg agttcattcg tgcgtttttc actgagaagc gtaaaagctg tttactttca
     6961 aaatcgatat catcgtcaat cacatagaac cgaacaaatt tagacgggtt gcaattatct
     7021 aatactgaga caaataatgc agctaaatgt ggtacgaatt ttgtgttaca acttgcgact
     7081 actgctattt cttttttatt cattgatctc gcctactttc gttagagtac tgtgtgctaa
     7141 tttttttaag tataaattgg tatacggatg atctttcaac gtattccaag gtttatcatg
     7201 tcctgtaaag tgaacgatcg aaggtttttc atttccttct ttatactgac gttcatattt
     7261 ttcatttggt gctggatgac gttcaaaaat cagcgaagtc tgcatgttcc attttggatg
     7321 aagttgttcc caatcttcat aaagtacagc attcaaagca tcttggtcgt gatagatgat
     7381 tcgatcgcca ttttcactga ggtaatggat agttttttct gtaatttctt ttttattcca
     7441 ttgatcaatg tcaatcacca tcactcctga attgaagtac aagtcatcgg aatcgattcc
     7501 taatctttcc aaagctttca cttggcctgg atcgataata gcgccgattg tttttccatt
     7561 taacggctca tcatatagtt ttacgatatc gtctagtact aacacatctg aatctaagta
     7621 aaggactttc ttataatcct ctttagctaa taagtttggt aaagaaattc ttaagtaagc
     7681 ggtagttgtg atatggtcac tgactaggaa gtcttcgtag atatcttttt ccactgtcag
     7741 gaattggata cttgcattac ttgaatattt atttaccgtt tcttccaatc cttctttact
     7801 atattctgat agaccatcgt caatcacata aaattttatg cgtcttgttt tattgcaatt
     7861 ttctaatgca gttgcgatca tcacgctcaa gtaaggagca taattttcgt ctgaagcagt
     7921 taccacagga actacgccgt atctcttctc catcaaaatc actccttttt ctaatctttt
     7981 tctaacctgt tctacatcta gaatattcct catttggttt taaagcaaaa tatatgcttt
     8041 gttttcattg aatattttaa aattttttta tcacaaaacg catcggtaag cgctaactat
     8101 tcgtttttca tggcttttta ttcaaaggta ttgaccaaaa actactatat atagccacct
     8161 ttttcttaag aatgtttttt gaaaaaattc ataatgacaa agcaagttag tttcattttt
     8221 ttaaattctc atttaatctt aaggtaacag gaaaatagat atgtaaaaag aaagtaggaa
     8281 gataaaatga aactagcagc gattgattta gacggtacct tgcttgatcg aaatggaaag
     8341 ataccagaag aaaatatcca agcacttcgg aaatttgatg aaaatggcgg aatcgtaacc
     8401 gtcgctaccg gaaggaacag catttctgct aaagatattt tttccgaatt aggaatcggt
     8461 ggctatttga tttcttccaa tggttcactg attgctgaaa tgaaggatgg aaaaatggcg
     8521 aaagttctca gacgttcaaa aatagatagc gatacactga gaaaagcctt cttctatgct
     8581 caagaagcga aaatatccat cattgctagc cgagaaaccc aagatgatca gctgacattc
     8641 gatgaatcaa atctagtcaa agatgaccct tattatgaac atttcaattt acagaaccat
     8701 tcttttgatc aaattttaaa tatgttggaa gatccttctc ttagctatct taaattagca
     8761 ttaacagata agagtgaaga aaaactacag aagctacaaa gagatctgca agcagatggt
     8821 atccaatcag tattctctga tccgcatttc ttagaaatca cgcctacagg tatcactaaa
     8881 gctcattcac ttgtttttct ggcggattac cttggaatcg aatcgagtga catcatggct
     8941 tttggcgatc aagaaaacga cattgctatg ttgaattaca gcgggttgag tattgcgatg
     9001 gggaatgcac agcgacaagt aaaagaaatt gcaaaccaag taacactgac gaatgaagaa
     9061 gctggtgttg cttatatttt aaaaaacttc tattaaaaag aataaaatga cgatatgaat
     9121 gtaggaggaa agaaaatgtt agttattatt gatatgcaaa atcatattct tgatccaaac
     9181 agcgaatttt atattccaga gtcagatcaa ttggcagaac gtattgctca aagattagct
     9241 aaagcacgcc aaaataatga atatattctc ttcacacggg atataccagt tgaactgaag
     9301 gacaaagaag aagaacgaga agatttgcaa ttgatccctc ttctttctcc acaagaaaat
     9361 gaacgagtga ttaaaaagta ttactttacg attccacctg aaactttagc tgaaatccag
     9421 gaaactttat ttgaaagcaa agaagaacaa aaagagatcg aagtagtcgg gattgaaaca
     9481 aacttgtgtg tactctctaa tctgataggc ctgcagagtg cttttcctga agcagacttt
     9541 tttgttgatc caatgttagt ctccagtaga aaacacggag aatccgcttt agagctgttg
     9601 aaagatttca acgtctctat cgtcgaatca taaaaatatc tatcttcatt tacgatattt
     9661 aacacacaat aaaaaaagca cgcagaactg ttttagtcct acgtgctttt tctatcaaag
     9721 tagattggct tttttatgac acagagtgaa acggcaggct cacgacctct ttcttaaatg
     9781 gtgttcaatc agctgaccct cggtattagc ttaacaaacg gcaaaatatg aggaactatt
     9841 tttccgtttg ttaagctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
     9901 cggtattcaa aagcttgcat ctgcagtaat aagaacagcc aagtaaaaac ataagaagct
     9961 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttatgt
    10021 accttgtagg actcgaacct acgaccgga
//
LOCUS       DAZBTH010000097         9970 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000097 DAZBTH010000000
VERSION     DAZBTH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.099"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACHGNW_002260"
     CDS             205..684
                     /locus_tag="ACHGNW_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN6926529.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACHGNW_002261"
     CDS             717..2945
                     /locus_tag="ACHGNW_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926530.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACHGNW_002262"
     CDS             3074..4717
                     /locus_tag="ACHGNW_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6926531.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACHGNW_002263"
     CDS             complement(4790..5335)
                     /locus_tag="ACHGNW_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN6926532.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACHGNW_002264"
     CDS             complement(5358..5843)
                     /locus_tag="ACHGNW_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6926533.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACHGNW_002265"
     CDS             complement(5833..6645)
                     /locus_tag="ACHGNW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN6926534.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACHGNW_002266"
     CDS             complement(6784..8316)
                     /locus_tag="ACHGNW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN6926535.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACHGNW_002267"
     CDS             complement(8425..9456)
                     /locus_tag="ACHGNW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926536.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3318 a         1842 c         1917 g         2893 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta
     9901 atcctttttt atataagttg tttgcttatt gatggattgg ataaattaaa ccatcacaac
     9961 gagtggaaat
//
LOCUS       DAZBTH010000098         9966 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000098 DAZBTH010000000
VERSION     DAZBTH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.100"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(430..654)
                     /locus_tag="ACHGNW_002268"
     CDS             complement(430..654)
                     /locus_tag="ACHGNW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926537.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            845..3622
                     /locus_tag="ACHGNW_002269"
     CDS             845..3622
                     /locus_tag="ACHGNW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6926538.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3703..4122
                     /locus_tag="ACHGNW_002270"
     CDS             3703..4122
                     /locus_tag="ACHGNW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6926539.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4134..4604
                     /locus_tag="ACHGNW_002271"
     CDS             4134..4604
                     /locus_tag="ACHGNW_002271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6926540.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4621..5400
                     /locus_tag="ACHGNW_002272"
     CDS             4621..5400
                     /locus_tag="ACHGNW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6926541.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5387..6208
                     /locus_tag="ACHGNW_002273"
     CDS             5387..6208
                     /locus_tag="ACHGNW_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6926542.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6225..7274
                     /locus_tag="ACHGNW_002274"
     CDS             6225..7274
                     /locus_tag="ACHGNW_002274"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6926543.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7287..8291
                     /locus_tag="ACHGNW_002275"
     CDS             7287..8291
                     /locus_tag="ACHGNW_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6926544.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8924..9943
                     /locus_tag="ACHGNW_002276"
     CDS             8924..9943
                     /locus_tag="ACHGNW_002276"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6926545.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3348 a         1646 c         2083 g         2889 t
ORIGIN      
        1 agaaatctaa ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta
       61 ttgtgggtac tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat
      121 tctatcaggt ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga
      181 ttttgatctg aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt
      241 tcgtaatcaa tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca
      301 acagtacctc tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa
      361 ttgttgttat accacatata aggatttgta atagctaaaa acagacagga aagttgaaat
      421 ctgtgaatct tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa
      481 taaaaatcct gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt
      541 catagccata taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc
      601 tcgaaaagtt ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata
      661 gtcatagcct ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg
      721 tacgtttttt agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata
      781 tttttcaatt ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg
      841 aggggtgaat aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt
      901 tttcgattta gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg
      961 ggtaaaaaga aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt
     1021 caaaatcaat tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt
     1081 taagttacgc acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt
     1141 cgcgcaacca aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag
     1201 tatcgaacaa atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac
     1261 aggcgaaagt ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct
     1321 tcatgatctg ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga
     1381 taatcctgaa cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc
     1441 agaagaaaat aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga
     1501 ggtacatcgc ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt
     1561 gatttatcgt atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc
     1621 tacgactgaa gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg
     1681 aatcgaacta cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc
     1741 tttttttctc gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt
     1801 gtcactttta acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa
     1861 agtttcagca gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat
     1921 ccatcatttg caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg
     1981 aatctatatt acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca
     2041 gcagcgaatc gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct
     2101 gctatattct tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct
     2161 gtttgagacc agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg
     2221 agagaccttt cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta
     2281 tgccttgagt tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga
     2341 tattatccaa ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta
     2401 tcactatgtc caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag
     2461 tatggatcgg attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg
     2521 actgccaaaa gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt
     2581 agtaaatcgt ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac
     2641 accacagcag attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg
     2701 attgatcata ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca
     2761 gttatccgta ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg
     2821 ggaatgcctg caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat
     2881 ccagccagaa tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac
     2941 ttgcttcact ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc
     3001 tacggcttgt cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa
     3061 cagtgaccca ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga
     3121 tatcaccaat atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt
     3181 actagctgaa tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg
     3241 catcattcgt aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga
     3301 gaaggtgatg ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag
     3361 catcagcaat cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt
     3421 gatccgacaa atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa
     3481 agaagggtta tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat
     3541 gatccccaat tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct
     3601 ctcgatagaa gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac
     3661 ttgctttttt agtatgtgta agtgaaaaga ggagatgaaa aagtggatag agaaattatc
     3721 ttagcttccc atggaaaatt tgcttcaggg atattagatt cattagagct gatctatggg
     3781 aaaaatcatt ctattactgt attcgattgc tatactgatg agaattttga tttagcggaa
     3841 acagtaaggc agctttttat gaggtacaaa gataaagaaa tcattgttct gacggatctt
     3901 tttggcggaa gtgtcaacaa cgaattctta caatatatca atcgggatca tgtctatctc
     3961 gttgccggat tgaatttgcc actattgatt gagctagtat ctcggttgga cgctgaagta
     4021 gagacaactg ttttgatcca gcaggtgcta actgattcaa aaaaaatgat ccagttttgt
     4081 aacgagcggc tcaatcagaa aatagaagaa gacgaatttt aggaggaaga aaaatgattg
     4141 tattgacaag agtcgaccat cggttattac acggacaagt agctttttca tggacacaaa
     4201 caataggcgc agactgtatt ttgattgcta atgatgatgt acctacgaat gagatacgaa
     4261 agacaacgat caaattagcc aagccacaag gagtcaaact agtgatcaaa tcaattgatg
     4321 actcgattgc cgcgttgaaa agcggggtga cagataaata taagttattc atcgtagtgg
     4381 aatcgatcga ggatgcatat aagctggcag aagcgtatcc agaaatcaaa accatcaact
     4441 taggagggat caaggcaaaa cctggtactc gcagcatcgg aaaagcggta aatattttac
     4501 cagaagagga ggaacgcttg aaacgattaa ttgacaaaca aatagaagta gaaatccgtc
     4561 aagtaccagc agataaaaaa gtggatgtaa gagaagtttt gtaagaaagg aagaacctca
     4621 atgattgtac aagcagtctt attaggaatt gttgcatttg tcgcacaatc agaatatgca
     4681 ctagggacat cgttgctttc tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta
     4741 ggtgatgtaa aaactgggat cattatgggc gcaactttag agttagcatt tatcggatcg
     4801 ttctcagtag gtgcttcgat tccacctgat gttgtgaccg gtgggatttt agggacggct
     4861 tttgctatta cagctggagc cggtacagaa accgcgcttt tactaggttt gccgattgct
     4921 acgttgacat tgatattgaa aaatatttac ttagggcttt tgattccaat tatgaaccat
     4981 aaagcagaca attatgcaga agaaggaaat tataaaggag tcgagcatat gcacttgctt
     5041 gccggtatcg gtctttcctt gatgcttggt gttgtggtaa tggtttctta tatggtggga
     5101 agcaatacga tcggaaatct attaaatatg attcctgact tcgttcaaaa ggggttgtca
     5161 gtagctacag ggttgattcc agctcttggt tttgcgatgc ttgctcgttt gctgatcaac
     5221 aaacaagttg caccatattt tttcttaggt tttgctatag cttcttactt agagattcct
     5281 gtgaccggag tagctatttt tggtgcgatt ttagcagttg tggtagtcaa tattatgaat
     5341 gtccgtcaac taccacttca aacaactgca ggggaggtag aagaagatga agaattctga
     5401 gatcaaaaac agcaaaataa cgaaaaaaga gctaaatcac gtattttggc gttcttttca
     5461 aatggagttt tcttggaatt atgaacgtca gatgaatatg gcttatgttt atgcaatgat
     5521 tccgattttg aaaaagttat atcagtcgaa agaagaaatg gcagcagctt tgaaacggca
     5581 tctagagttt tttaacacga ccccacatat cgttacactg attttaggaa tcacggctgc
     5641 aatggaggaa gaaaataagg atgatcctga atttgatgtg actgcgatcg atagtatcaa
     5701 aacatctttg atgggacctc ttgcagggtt aggagattct ttcttttggg gaactttgcg
     5761 attgattgcc acaggtgtgg gtacctcttt agccatgcaa ggaaatattt taggccctat
     5821 cttgttcatt ttgattttca atattccgca cattctgttt cgctatctag caacaggttg
     5881 gggatatcgc ttaggaacgg gttttttgaa aaagatccag gcaaacggta tgatggaaag
     5941 tttgacatta ggcgcgtcta ttattggact gatggtcgtt ggagcaatga ctgccacgat
     6001 gatcaccatc aatattcctt taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag
     6061 tatttttgat gatatcgttc caaatatctt atgtcttgga gcatttggca tagtgtttta
     6121 tctattgaag aaagaagtga aaccattaac gatcttgatc ggactagctt tctttggaat
     6181 ttttggttcg tggataggga ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag
     6241 attatatcta tgaagaaaag gaagtactaa gtacgatctt gaaaaagaat gatttttcga
     6301 caagagaaca tatgaaaaaa acagtgaatt tactgatctt agcaacgggt tcatcttata
     6361 acgcttgttt agcagcgaag ccagcattgg aatcttatgg tgatcttact gtcgatattc
     6421 aagaaccttt ttattttttc aattatggaa aactttctcc ttcaatcgat actgtgattg
     6481 ctgtgtcaca aagcgggaaa agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac
     6541 aagggttacc tgttgttgct atcacaaatg acgtgcaaag tccacttgct ttagaagctg
     6601 cacaaattat tgatttaggt gccggggtag aatcagtagg cttcgttaca aaaggctatt
     6661 ctgcgactgt tttacagtta ctactcttag ggataggaat tggaataagt aaaaataaaa
     6721 tcagcaaaaa aatagaacaa gactacatgc aacagttgcg caaaataatc aaccatttac
     6781 cagcgattat tcaaaaaacg gaagaatttt ttgatggata tcaaagttta tttcgtttag
     6841 cacagcgttt cgtcaccatt ggatatggtc caaactgggg aacagctaaa gaagcagaga
     6901 cgaaattgac tgaaacaatc cgcgttccgt cacaaggatt tgagttagaa gcttatatgc
     6961 acggcccata cctggaggca gatgcttcgc atttgctatt ctttattgaa ggggaaagtg
     7021 taaataagga acgttcgcaa aagttgcaaa gatatatgag tcggtacgtt ggagagactc
     7081 tcaccattac cacaaaaaaa gcaagaaacg agaaaacact tggattagca atagaatgtg
     7141 atgaatatct gtctgtatta gcgcttgtcg ttccgtttca actattcgct tataaaaccg
     7201 cagtagctaa aggtattgac ttgaacaaaa agatttttga agactttgat acagtcttga
     7261 aaagtaaatt ataagaggtg gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag
     7321 caataaaagg cgcactagct ttacgtccgc aagtagaaga aatcatcgat aaactttatg
     7381 cggaaaaatt tgacgctgtc tattatctag gaatcggtgg aacgtatgct tcctcgatgc
     7441 aagctgtcac ttatatgaat gggaaaagca acttgcctgt ctttgtacag catgctgcag
     7501 aatattacac tactggaaac aaaagattaa caaaagattc tatcgttatt ttgtcctctg
     7561 ttaccggtac aactcaagaa gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga
     7621 cactgattgg atttatcgat aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg
     7681 tcacttatcc agctcctgga acagaacaaa tcaaattttt tatggcagca gatcgattga
     7741 tgtatttgaa tggagaattc gaagcttatt cagaatacta tgaacagctc gaacgttatt
     7801 taccaacagg gttagtggaa gcagagaaaa aagcagatgc atttggctta tcatttgcag
     7861 aaaaacatcg acgtgatagt atgcattatt ttattggagc agggaatcaa tggggagctg
     7921 tttattcata tgccatgtgt tactgggaag aacaaagctg gcttccatcg aaatctatcc
     7981 atgctgccga gtttcttcac gggacgttag agatcgtaga agaaacaacc ccagtcacat
     8041 tatttttagg cgaagatgaa cagagagtgt tatctgaacg tgtggctaaa cttttgccaa
     8101 aaatttgcag caattataca ttgatagaca caaaagacta tccagtagaa ggtatcagtg
     8161 aaaaatatcg cggacgtgtt ttgtctttct tattgatgca tgtagtgaca caacgcatcg
     8221 atgctcatgt ggagcagttg aactgccatc cattagagat acgccgctac tatcgtcagt
     8281 ttgattatta ggatagactg gataaaaaga atataaaaaa taaagagagc agcgataact
     8341 gctcccttta tttttttaaa atattggcat caattacgtt ataaaacaca ttttttgcat
     8401 tatctacagt tcaacttata atctgtttaa tgggacatgt gggtgccaac cattttttgt
     8461 tatgcaatag tactatttag ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag
     8521 ttgcgccagt ttacatagcg ttttgtatcc agaatgttga ctaaaacaaa agttcctatg
     8581 tcaaccgtca acagagacaa tctgtccgtt gcaggacctg acagatttag aaatgttcaa
     8641 tctgtctctt atgtatttta aatcaatact cactaggaga agtttcctaa cctcctaata
     8701 gtaatgctgg tagtaacatt gtcaaaactg cttcctctta ggaacaagca aagcataacg
     8761 agtgtaaaaa taaaatgtag actattgtaa catatcattg attatttcaa gtaaaaacga
     8821 taaaagaagt aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta
     8881 tactataatg gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc
     8941 agttcttggt cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca
     9001 tgatgtccgt atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac
     9061 aaatcagcat tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt
     9121 ggcagaagca gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg
     9181 ttcagtcgca caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag
     9241 taaaggacta gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc
     9301 agaagaaaaa agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc
     9361 agtacatgat atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt
     9421 acaaaaactg ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt
     9481 agaaactggt gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt
     9541 aggattcgga gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg
     9601 tcttggtgtc gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga
     9661 cttgatcgtt acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg
     9721 acagggacat aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc
     9781 aacgacaaaa gcagcagtag agctagctca acagctaggt gttgagatgc caattactca
     9841 aacaatctat aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat
     9901 gttacgtgac ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt
     9961 aatcgt
//
LOCUS       DAZBTH010000099         9501 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000099 DAZBTH010000000
VERSION     DAZBTH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.101"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..283
                     /locus_tag="ACHGNW_002277"
     CDS             89..283
                     /locus_tag="ACHGNW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926546.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            552..851
                     /locus_tag="ACHGNW_002278"
     CDS             552..851
                     /locus_tag="ACHGNW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926547.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(948..1382)
                     /locus_tag="ACHGNW_002279"
     CDS             complement(948..1382)
                     /locus_tag="ACHGNW_002279"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926548.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1614..2324)
                     /locus_tag="ACHGNW_002280"
     CDS             complement(1614..2324)
                     /locus_tag="ACHGNW_002280"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6926549.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2314..3174)
                     /locus_tag="ACHGNW_002281"
     CDS             complement(2314..3174)
                     /locus_tag="ACHGNW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN6926550.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3179..3817)
                     /locus_tag="ACHGNW_002282"
     CDS             complement(3179..3817)
                     /locus_tag="ACHGNW_002282"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN6926551.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3832..4131)
                     /locus_tag="ACHGNW_002283"
     CDS             complement(3832..4131)
                     /locus_tag="ACHGNW_002283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6926552.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4174..5658)
                     /locus_tag="ACHGNW_002284"
     CDS             complement(4174..5658)
                     /locus_tag="ACHGNW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6926553.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5678..6139)
                     /locus_tag="ACHGNW_002285"
     CDS             complement(5678..6139)
                     /locus_tag="ACHGNW_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6926554.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHGNW_002286"
     CDS             complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHGNW_002286"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN6926555.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7442..8212
                     /locus_tag="ACHGNW_002287"
     CDS             7442..8212
                     /locus_tag="ACHGNW_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6926556.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8485..9312)
                     /locus_tag="ACHGNW_002288"
     CDS             complement(8485..9312)
                     /locus_tag="ACHGNW_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926557.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2958 a         1942 c         1488 g         3113 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      721 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      781 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      841 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      901 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      961 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
     1021 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1081 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1141 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1201 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1261 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1321 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1381 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1441 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1501 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1561 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1621 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1681 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1741 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1801 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1861 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1921 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1981 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     2041 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2101 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2161 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2221 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2281 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2341 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2401 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2461 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2521 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2581 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2641 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2701 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2761 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2821 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2881 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2941 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     3001 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3061 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3121 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3181 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3241 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3301 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3361 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3421 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3481 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3541 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3601 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3661 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3721 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3781 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3841 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3901 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3961 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     4021 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4081 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4141 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4201 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4261 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4321 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4381 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4441 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4501 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4561 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4621 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4681 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4741 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4801 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4861 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4921 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4981 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     5041 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5101 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5161 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5221 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5281 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5341 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5401 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5461 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5521 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5581 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5641 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5701 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5761 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5821 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5881 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5941 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     6001 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6061 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6121 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6181 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6241 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6301 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6361 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6421 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6481 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6541 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6601 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6661 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6721 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6781 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6841 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6901 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6961 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     7021 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7081 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7141 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7201 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7261 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7321 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7381 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7441 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7501 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7561 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7621 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7681 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7741 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7801 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7861 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7921 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7981 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     8041 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8101 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8161 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8221 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8281 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8341 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8401 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8461 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8521 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8581 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8641 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8701 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8761 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8821 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8881 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8941 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     9001 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
     9061 tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt cttctgtttc
     9121 tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta agatattgat
     9181 gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac aaatgccttc
     9241 tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta atgctccatc
     9301 tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta tatcatacac
     9361 aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt aattttaaaa
     9421 taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt gttatctgaa
     9481 taattatcgt ttacagttat a
//
LOCUS       DAZBTH010000100         9349 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000100 DAZBTH010000000
VERSION     DAZBTH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.102"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..748)
                     /locus_tag="ACHGNW_002289"
     CDS             complement(80..748)
                     /locus_tag="ACHGNW_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6926558.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            882..1169
                     /locus_tag="ACHGNW_002290"
     CDS             882..1169
                     /locus_tag="ACHGNW_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926559.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1585..3123
                     /locus_tag="ACHGNW_002291"
     CDS             1585..3123
                     /locus_tag="ACHGNW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN6926560.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3120..4085
                     /locus_tag="ACHGNW_002292"
     CDS             3120..4085
                     /locus_tag="ACHGNW_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926561.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4072..5394
                     /locus_tag="ACHGNW_002293"
     CDS             4072..5394
                     /locus_tag="ACHGNW_002293"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6926562.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5457..5561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5635..6378
                     /locus_tag="ACHGNW_002294"
     CDS             5635..6378
                     /locus_tag="ACHGNW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926563.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6393..7385
                     /locus_tag="ACHGNW_002295"
     CDS             6393..7385
                     /locus_tag="ACHGNW_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6926564.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7397..8134
                     /locus_tag="ACHGNW_002296"
     CDS             7397..8134
                     /locus_tag="ACHGNW_002296"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926565.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8124..8846
                     /locus_tag="ACHGNW_002297"
     CDS             8124..8846
                     /locus_tag="ACHGNW_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6926566.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8930..>9349)
                     /locus_tag="ACHGNW_002298"
     CDS             complement(8930..>9349)
                     /locus_tag="ACHGNW_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6926567.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3064 a         1607 c         1972 g         2706 t
ORIGIN      
        1 gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt aaattaaata
       61 aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt ggttcaaatg
      121 tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc agatacttca
      181 ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga aaatgtgtga
      241 gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa agacttttgc
      301 caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc
      361 ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct gtcatcaaac
      421 tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat aaatgtaaaa
      481 agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa
      541 acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc
      601 caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc agcttagatg
      661 gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg attccgcaca
      721 attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa aattttcagc
      781 aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa aaaaattatt
      841 tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca gcagacttat
      901 tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt ttggctcaag
      961 aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa tttatctttt
     1021 tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt ttgatgaaga
     1081 accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt tatggagtga
     1141 aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac gtttatgatg
     1201 tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt ataatattcg
     1261 ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg gctgtttctt
     1321 tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc
     1381 gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt
     1441 gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg
     1501 tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta
     1561 aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa
     1621 ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt
     1681 gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa
     1741 gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta
     1801 cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct
     1861 ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa
     1921 gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc
     1981 atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg
     2041 gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt
     2101 gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt
     2161 atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact
     2221 ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa
     2281 aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg
     2341 tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc
     2401 aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc
     2461 acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg
     2521 acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca
     2581 ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg
     2641 aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg
     2701 ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat
     2761 ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat
     2821 aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat
     2881 ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg
     2941 aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga
     3001 aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag
     3061 ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag
     3121 tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg
     3181 atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta
     3241 gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca
     3301 tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg
     3361 gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag
     3421 tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc
     3481 ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg
     3541 gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt
     3601 tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg
     3661 ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag
     3721 aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg
     3781 acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg
     3841 aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa
     3901 atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat
     3961 attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt
     4021 cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca
     4081 tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa
     4141 gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac
     4201 cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa
     4261 caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa
     4321 ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct
     4381 gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac
     4441 gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta
     4501 ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg
     4561 cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc
     4621 aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt
     4681 gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct
     4741 tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa
     4801 gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa
     4861 tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat
     4921 tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc
     4981 ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat
     5041 gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa
     5101 cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg
     5161 gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg
     5221 acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa
     5281 ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc
     5341 tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca
     5401 tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc
     5461 tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc
     5521 tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc
     5581 gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag
     5641 aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc
     5701 atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta
     5761 gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt
     5821 ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag
     5881 tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg
     5941 ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt
     6001 gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca
     6061 aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata
     6121 tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat
     6181 tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac
     6241 atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc
     6301 attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt
     6361 atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc
     6421 tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa
     6481 aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca
     6541 tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg
     6601 aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg
     6661 taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag
     6721 caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca
     6781 tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag
     6841 aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg
     6901 taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact
     6961 ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat
     7021 tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg aatgatcaac
     7081 tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc
     7141 aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa
     7201 atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc
     7261 ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg
     7321 aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa
     7381 aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct
     7441 gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc
     7501 gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac
     7561 ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta
     7621 tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata
     7681 tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt
     7741 tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg
     7801 aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt
     7861 tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa
     7921 aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc
     7981 agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc
     8041 tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta
     8101 tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc
     8161 agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc
     8221 cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg
     8281 gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa
     8341 aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta
     8401 cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg
     8461 tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc
     8521 actatttgat ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct
     8581 gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag
     8641 cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt
     8701 taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa
     8761 tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg
     8821 aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct
     8881 ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct tagtaccaac
     8941 cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt
     9001 gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa gaagaatcta
     9061 attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt
     9121 gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt tctgttacag
     9181 caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga accgtttctt
     9241 gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag
     9301 ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAZBTH010000101         9285 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000101 DAZBTH010000000
VERSION     DAZBTH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.103"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..320)
                     /locus_tag="ACHGNW_002299"
     CDS             complement(<1..320)
                     /locus_tag="ACHGNW_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN6926568.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVK"
     gene            complement(310..2484)
                     /locus_tag="ACHGNW_002300"
     CDS             complement(310..2484)
                     /locus_tag="ACHGNW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFN6926569.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2500..4842)
                     /locus_tag="ACHGNW_002301"
     CDS             complement(2500..4842)
                     /locus_tag="ACHGNW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6926570.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4864..7407)
                     /locus_tag="ACHGNW_002302"
     CDS             complement(4864..7407)
                     /locus_tag="ACHGNW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6926571.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7417..7815)
                     /locus_tag="ACHGNW_002303"
     CDS             complement(7417..7815)
                     /locus_tag="ACHGNW_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN6926572.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7896..8180)
                     /locus_tag="ACHGNW_002304"
     CDS             complement(7896..8180)
                     /locus_tag="ACHGNW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926573.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8204..9202)
                     /locus_tag="ACHGNW_002305"
     CDS             complement(8204..9202)
                     /locus_tag="ACHGNW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6926574.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2638 a         1993 c         1453 g         3201 t
ORIGIN      
        1 cttttaacat ggtaacgttg taaaaaagtt tgaagttctt cttgtcttgg ttcttttctt
       61 gtttcaattc gatatacatt cctagagaat tttttgtcgt atctttccaa gaaagattca
      121 acttcttggc attttttaca atcatttctt gctagataca caaaaaatga ttgattcgtt
      181 ttttgaagtt ctagtacttc ttctaaatta ctattgatta gttttccatt tgattcttcg
      241 ttagttttta tcagtttaaa acaaccaaat aagaggaaac taacaataag tatcctaaca
      301 ataactttct tatttcgcat attgtttctc caaatttggt ttccgaatat agacgactcg
      361 cggtggttcc tggacgtctt cagctgttcg atacaccaca aattcgcctg ttctcaatgc
      421 tcgaatgcga ttcggattga atttaaagcg gtctacgtct cgtttcgtgc ctctttctcc
      481 tgtccaatca acctttccca tatcagaata acctgcttct tgtgtcgtca tttccgttaa
      541 ttcaatgtct tcttctgtcc ccattaaatc tgcccaataa tcaatttctg tcgggtcatt
      601 tgtcttccca ataaagaacg tattcacatt cccaatcaac tgttttcgaa ggacaggatc
      661 aattctatca atgtccgcaa gcgtttgagg ggcatacgcc gcatgcaccc cagcgcctcg
      721 tcccttattg actaagtcta taaggttatc atttagataa gaagctggtt cgtcaaatat
      781 tactaggact ttcgagcgat tggctgtttc tcctgcttgt ttatacactt ctgacgcaaa
      841 gaaattaata tctcccacaa gcatttgagc caatgtccga ataaactcac tataaatcaa
      901 tccgtttaac gagatataaa caatctttcc ttctttaaca acttcttgaa tatctaattc
      961 tctttttcct ccagaagtat caaagagttc accaagctca ctatccaata acatattgat
     1021 attggtctgt aaattctcag aagtcgcata caaataaaac gggttcttct tatgttcgta
     1081 acgaacaaac aaccgtttaa acaacgtttg ctcgatttct gtcatgagag aaatgttttc
     1141 ttttaataat agataatacg tttctaagtc aacggtttta cgatcatgtt caacgattcg
     1201 ctcttcactt aactgttcca catcggcaaa atcacgttct gttttgtcca tcgcttcatt
     1261 ctttttcgtt tttttcttcg tggttttctt cttttctacg accacttttt ctttaaataa
     1321 atctggattt ttcaactgaa tacgatccgc aaacaaatgt aagacgtttc gtggcaataa
     1381 ataatgttgt aaccaaacta gatttctttc aattttatgg ctcttttgga actcgtctaa
     1441 aagctgaatc gttacctgta ataacgcttt ggctgctgtc gaataataca cgctttctgt
     1501 ttctgctaga ttggttaatt tatctcgaat ggcaatatca ttgccatttt ttacaggatt
     1561 ataacgcaaa tcgccaccgt ccacaaagac ttcaatcggg cgattgtatg cctctgccac
     1621 ttcttttaac gcttgtaaag tctctcctgc tcctttccca tcgactaaga taacggggat
     1681 acgaaactta cacgcatgtt ctgctagtaa ttggatcacc gtcgtttttc ctgaacctgt
     1741 agtcgctgga ataatcacat gttggttcaa ttcactaaag ttcatcacat aagggagccc
     1801 atactcatct gttcctaata gaatcacttt ggctaatgaa gccagttgtt tattccgcaa
     1861 agcaacgtct tttatgacaa ttgctacctg gtacgcttgc tgatactctg ctgtatattt
     1921 ttgggactcc tgaaatttat tcttccgacg ttccttgtat tctttactat ttagcaaagc
     1981 tgtttgccgt tctttctttg aaacaacttt tcttttttca ataaaagacg catacgcaat
     2041 caaacaactt cctgcacctg tcccacctaa aaggagggca aaaatcgttt gtccattaaa
     2101 ctgcaacgca tgagaaaaca agtttaatgg aaataataag taacacgaca aataccctct
     2161 aggaagaaca aaaataaaac aaaatagatt taatagcgta aaacctaacg caaaaataaa
     2221 aaaaagagag cttcttcgtt ttaagaagct ctcatactca aagtcataaa tatgtgcacg
     2281 tgtctctttt ttatctatca taaagacaat cgctaaagga atactgccta caataaaaaa
     2341 tggatataga caaacaccta ataaagtgac gataaaataa agttgttgaa accgactttt
     2401 tggcgtttct ttttcttgtt ttcttttgcg atcgacagaa aacatttgct caaaatgtgt
     2461 cggtcgtttt ggtgcattca tcatactatc gatttcctcc tatcgttttc gttgtttcat
     2521 ttctcttcga taaatcggtt gtttttctcg ttttcgtttc atttttgctt gagttgctcg
     2581 aaactcactc cgtgtcttcg gtgcttgttt tggtgatttt gtttgtttca tggttgagta
     2641 tgctttagat acggttgaag gcgctttggt ttgtgctgtt actcgattga ctgctgacat
     2701 atgttcccga accccctctg gacgtcgaac agatttcggt gtggtagaac ctgttgctcg
     2761 tggtacattc ccttttctta gtggtttttg taccgcatgt ttttgtcctg caactgtccg
     2821 ggttttcatc gaacttgttt ttgttacggg agtaagtgcc tccttccgac gacgacttgt
     2881 ttgtccctga cgacgttttc gatcgttcaa ttctgttcta ggcgtatgct tctggccata
     2941 agacttgtgt ttttgctttg gattcgctaa tgacaccacc attgcagaac cctgattgcg
     3001 atacggacta cgagacgcac cttttctact tctttctctt agactaggat tgccttgcaa
     3061 ttctcgttgt aataaatcgg ctgtttttct tgcctgtttc tccattgctg ttcgttgcat
     3121 acgttcttga ctttgctctt ctgcttgttt cgcttgatca tgcaagtctc ttgctttttg
     3181 tgccatacgt tccatacgat ttggtgcttt atgacgaggt tgttgtgcgg attcacgccg
     3241 aaaagcactt cgtgtggctt gtttcgccgt ttgtggcata cgaacgtctg agccaccccc
     3301 acgttttcgt tgacgttctt gtctcttccg tgcgttttct ttttcttttt gtttctgtgc
     3361 ttttctcgct tctttgcgtt ctttcaaccg ttgtttggct tcccttctca attttgcttc
     3421 tgccccaaat ggatttcccg caaaagctaa cgctcctcga acacccattc ttccagtaaa
     3481 tagactgcct aaaaagcctc ggaacaaata aattcccaac ggcgcaaaca aataaacaat
     3541 gatcaagaga attttttccc atggatcgtc ttgtgtacta cttaattgga acccgtaact
     3601 aagaaaagaa gaaaaaagga tacatgcaaa acctgtcatt ccttttaaaa acgtcaaaat
     3661 accaaaaact ttggcatagt taccaaacac attcattgtg acaagaaaca atcctaaaaa
     3721 aagtaagaac ggcaacatcg gtactaacaa tagttgcata gttccaataa tcaatcgaaa
     3781 catacttaag acaaaataaa caacggtttg aatcagattc acgacaaaat aaaacaaggc
     3841 aaatgctgta tttttccaac tattgtaata ttgtatgttc ttgtttttca gtgtatctgc
     3901 ttcatactct gtgactttgt catgaatttt ctcccctatt tttgtaccta aatcattgtc
     3961 caataaaaga ttgattcgat catattcttt tccagcatat ttgactttct tctcacgaat
     4021 tgtgtctaca ttggttgtgc cataatttaa caacagatat ggttcataaa cattactata
     4081 aaagacccga gccgccatca atttgcctgc cgtttccact tttttatccg ctgttacctc
     4141 ttctgtttct tttgtttcat aatttgtttc ttcttcaggt gttaacactg gattgacctt
     4201 cacgaacgcc gtttccactg tagaatctaa agaaaaaaga acatcaaaaa gattcttatt
     4261 tgtcccagaa tcttttaata cgcttaaccc agtaaaaata aggatcgtca taagaaagac
     4321 acggagtgct tgtttcattc gagcctctcc agcatatttg attgccatca taattgccga
     4381 aaaactgatt ccaatagttc ctgcaatact taacatattg tccgcaattt tttgtgttaa
     4441 gttttgaatg ggttctcgaa tggcggaaat aatatctaat tcaaagagaa attcaaccat
     4501 tgaagcattg aattctccta acatttttac gccactccaa acaaaatttt tcatcactcc
     4561 tgcaacattg tacgatagct tttttttaag gttcattgac tcatagtcat tggtcatcaa
     4621 ttcaaacgaa ttctttttgt aatttttata caattgatcc acatcagtcg ttacttcttt
     4681 tacttctact ttatcgttgg cattctctcc ttcagaacga tcggtttcag tgacacccgc
     4741 accatcattc atttgatcgg cagaaacagt agccccttgt agacagaaaa aaagaccccc
     4801 taataaaagg agcgtccacc atttcagaga tttttttcgc aaaaaaacca cctctttttt
     4861 gttttatcgt tgtttttcat tttctaacat tgcttgaatt ttttcatctt gattttcagt
     4921 agaagaaaca gccaataacc aactatcaaa caacacatca atggcaatcg gttgatttct
     4981 tcccaaatga tcctggaata aacaggttcc tgtttgtaat tgttccatca tttctaaatt
     5041 ggctgaattc acttccatat caaagaagga caatagtttc tctcgtgctt ctttttgttt
     5101 cggacgaaac gcaaatttgt aagaaattaa ctctttttta tcttcttgat cgtaatcttt
     5161 aaacgcttgc acaacaaggt aaatatccgt attattcgca cgtccttttc gcaagctatc
     5221 atcaatcaaa tagcttcctt gtgcggtatc ttcataccct tttgcctcgt cataaataat
     5281 cgcagcgtct tcttctttgt ttgtagaaaa gatatacgta aacttcataa tgacttccat
     5341 aattgccatt cctgcaagtt gttcgctatt taatcgaccc gaatcaatct ctttttgtgt
     5401 cggaatgcgt attccttgtg tccctaacac gttgatttgt gaatcaaaac gtatcggcgt
     5461 actgtgctca ttcccaatga gaatctttcc taaaccaacg ctatgcccta aaatcaattt
     5521 agctagttct ttgtcacttt ttcgaatttc ttcgataact tgacttaacg aatgcttacc
     5581 tggcatggtg taaactacat cgatggcgtc ttggatcacc gttttcttgt ggctcgctgt
     5641 ttctcgattg gtatctactt ctcctaacat tcctaacaca tttttagcgg tttgaattgc
     5701 ctcttctttt ggtaaaaaca atagaggatc aagcatccct cgacagcttt catctgaaga
     5761 taacgaaata aaattaattc gttcatacat agccacaaac tctggtatgt gtccgaattt
     5821 ttcaagtgcc tttaaaaaga acttttgggt ttcatttttc ggatcaatat aaagaatttt
     5881 ttgacctaaa aacatcagcc ataaaaaaat atattttaca aggaccgatt ttcctttacc
     5941 tggtggacca ataatcaacg tatttccatt ggtatgttcc gcgccttcaa tacttttctt
     6001 tgttaaattg ggataatacc atactagttt actagatagc gctaacgctt ctttcgcaga
     6061 tttaaacttt tttgcaataa caatccgtcc taaaggaaat cctttcttat ttcctacttc
     6121 tttctttaag tccaagccat aatctgccac aaaacctgtc gtagcgacct gctcgtaact
     6181 acgaaaacgt tgcgtacttc caattaagca ttgattaaac aacgttagct gatccgctac
     6241 cgatcgataa aatttgaaat ctgtagactc aatcgcaaat tcaatatctt tgactcgttc
     6301 ttccaattct tcttttgaag aagcagatac aaccagcgtg acggtagaat aacacaatcg
     6361 tttttctttg cgcttcagct tatcttttac atccattaac cgttctgtac caaaatttaa
     6421 tacatcgtct gaatctaaaa tgccgtcaac ggtctcatat tctttttctt gttcataaat
     6481 ccgtccagcc attttattaa tttttcgttc atcagatttt gaatgctcaa atcgaagtcg
     6541 aatatgcgta tcaattggaa aagataagct atcctgaaga ttttgaataa agctactccc
     6601 atacatggct aaaggagcgt ccgtttgact taaaaacgtc gaataatgcg tttgattcaa
     6661 ctgttcaatc gcaatgtatc ctgcttcatt cgaaatgacg ccctcgcaaa tttccattgg
     6721 cgtaagctgt ctttcaggca atttttcttc tgatcgatgg aaaaaataat aataaatttg
     6781 tccaatctct tgggcagata agcgctctat cggcttataa ttggacaatt ccgtatagag
     6841 ctccttttct aatgtttcat acgttggtaa cacactactt ataggaactc gttgtcccgt
     6901 aatacgatag ataaactttt taaaaacatg cttaagcaat tgaaaatatt cgatcggatc
     6961 agacactttc atttctgtag tgaaacgcac aaacagatac gttcgatact cgtttaattg
     7021 cacttcgtct tttaaaatct ttcctgcctg ttcaaagtac actttcccaa gatctgcgca
     7081 ttctcccttt accaaatctc gaatggtttc ttcaatgtgc gtatctaggt taaattgacg
     7141 tggaatattg ataaaatgat agtcatattc atcatgtcca agtatgcctt cgccatcttg
     7201 aagatagttc ttgaaaaaat caatgtcatt taaaggaaat acttgggaag aaagttgata
     7261 gcctacccac acatcatcat ttttggtcaa aacaaaattt cggataatgg cgtctatcgg
     7321 aaattctaat cgaggaagct tcgttttttt ctttttgaac tgccaaatcc cgataataaa
     7381 gacgaccaaa agaaacaaaa acgccattta ctcgactcac tccttccatt tgtgtaggga
     7441 atacgtcact ttttcatgga aaaacggcac atacatttca tgttcaaagc gttgtttccg
     7501 tttctgataa taggacgttc gcccaattaa atactcgaaa aagtgtttat tgtcccattt
     7561 cattgaaaac aatgtccaaa cgagaacacc aatcccaaaa atcataatca accaggactt
     7621 tcgaaacagt gtctgcaaga aactaatttg attcacatac gcaatcaaaa ataggatcaa
     7681 aagaatgaag agaaataccc cacctatgac taattgttcc atgcgaatgc cattagaaat
     7741 ccagggaatt ttttgtcctt taatcccgta aattttgtga gggtgcttaa actcctttgt
     7801 atagttgtat tccatgttca ctctacctcc ttttacaaaa aaagaagcga ccagaagttc
     7861 taatcttctg atcgcttttg cagtttgtct ttttcttatg ataattgtat ccagggcaca
     7921 atcatttgcg ttacccccac gttctcttca ctatccttaa taaagataat gttctttagc
     7981 cattcaccaa tatcaacggc attcgataac aataagacga gcattatcaa aataaagacg
     8041 ccaataatgg caaacgctgc ccgcgcaaag ccttgggtcg cataagcacc aaccattaga
     8101 acaatcgcaa cgattgccaa tagccaaatg acttgtccag atacatcatc aaataacgat
     8161 ttaagatcaa aaagtttcat ttccattctc ctctaagttc attttattca tttaacgtag
     8221 taacaaaata actatcgcca cttgttttta cttccattga aaagtttgat tggaactcgt
     8281 ttcctttctc aaaatagaaa cgatacttcc cttgcaaaaa gtagtttcct tctttatttt
     8341 ttactgcatg atccagttgt acattgatca attcgccgcc ttctaaccct ttttcgttag
     8401 aaatcaatgc caatttttca tcatttttga catataactc aaaaaaccgt ttcacaaatg
     8461 cttccagctt gatttcttcg ctttcagtga ccgcttgccc agaagaataa aatcgatttt
     8521 cctgatagtt cacagaattg gtattcttac tctcagaaag attcaaaaat tgaacgccat
     8581 caagtacttt taaaacattg ttttgataac tgactggaaa agaaagttga ctgcttttcc
     8641 aatgttctcc ttctgtaaaa gtaacctcat aaaataggcg atacattgct tgtgtcaaat
     8701 cgatttcttc tagtttaata aagcgaacat ctttcgctac tcgattggta gccgtcgtta
     8761 caaagcctag atcagacgct tccaatcctt gtgctaaata atgattgacg gaagcctccc
     8821 attcgtttcg tttttctggc gaataagtaa ataacctttc aataaaatgt tgtccctcgt
     8881 acttcaattg ttccgtagaa gacaaggttt ggttgacttc agttaacaat gtttctggat
     8941 tgacttgttt tttcactgct aaacgcgtta atgtatccac tcgtccaaag tttaggagga
     9001 gcacgacacc cattaacaga aacaaacaaa aaaaagcgcc tgaaaataca ctcatggcac
     9061 ttttttgcga aaattggatc attcgatctg acggttctgt tttctttttt tggaacaaaa
     9121 aacgtttctt ttgtttcttt tccttctgtt gtttcggtgg cttagtttgg aacgcttgtg
     9181 gattaaattt tgcattactc atgagaaatg ccataccgtt ctaacaataa tttcggtatt
     9241 ttgtccacaa tcactacttc gtggacaacc acacttgccc cctcc
//
LOCUS       DAZBTH010000102         9281 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000102 DAZBTH010000000
VERSION     DAZBTH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.104"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHGNW_002306"
     CDS             <1..435
                     /locus_tag="ACHGNW_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6926575.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHGNW_002307"
     tRNA            complement(759..842)
                     /locus_tag="ACHGNW_002307"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHGNW_002308"
     CDS             1370..1870
                     /locus_tag="ACHGNW_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926576.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHGNW_002309"
     CDS             complement(2060..2404)
                     /locus_tag="ACHGNW_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN6926577.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHGNW_002310"
     CDS             complement(2558..3478)
                     /locus_tag="ACHGNW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6926578.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHGNW_002311"
     CDS             3665..4156
                     /locus_tag="ACHGNW_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN6926579.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHGNW_002312"
     CDS             complement(4180..6555)
                     /locus_tag="ACHGNW_002312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN6926580.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHGNW_002313"
     CDS             6881..7507
                     /locus_tag="ACHGNW_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6926581.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHGNW_002314"
     CDS             7530..8234
                     /locus_tag="ACHGNW_002314"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6926582.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHGNW_002315"
     CDS             8426..9259
                     /locus_tag="ACHGNW_002315"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN6926583.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAZBTH010000103         8675 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000103 DAZBTH010000000
VERSION     DAZBTH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.105"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHGNW_002316"
     CDS             <1..1101
                     /locus_tag="ACHGNW_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6926584.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHGNW_002317"
     CDS             complement(1280..1615)
                     /locus_tag="ACHGNW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6926585.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHGNW_002318"
     CDS             complement(1599..1862)
                     /locus_tag="ACHGNW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN6926586.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHGNW_002319"
     CDS             complement(2322..3125)
                     /locus_tag="ACHGNW_002319"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6926587.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHGNW_002320"
     CDS             complement(3103..4227)
                     /locus_tag="ACHGNW_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926588.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHGNW_002321"
     CDS             complement(4243..5058)
                     /locus_tag="ACHGNW_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6926589.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHGNW_002322"
     CDS             complement(5048..5920)
                     /locus_tag="ACHGNW_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6926590.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHGNW_002323"
     CDS             complement(6005..7294)
                     /locus_tag="ACHGNW_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6926591.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHGNW_002324"
     CDS             complement(7438..7950)
                     /locus_tag="ACHGNW_002324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926592.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHGNW_002325"
     CDS             complement(8017..8160)
                     /locus_tag="ACHGNW_002325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6926593.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZBTH010000104         8574 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000104 DAZBTH010000000
VERSION     DAZBTH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.106"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ACHGNW_002326"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ACHGNW_002326"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN6926594.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHGNW_002327"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHGNW_002327"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN6926595.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="ACHGNW_002328"
     CDS             complement(3072..4325)
                     /locus_tag="ACHGNW_002328"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN6926596.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="ACHGNW_002329"
     CDS             complement(4331..5254)
                     /locus_tag="ACHGNW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN6926597.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="ACHGNW_002330"
     CDS             complement(5475..7967)
                     /locus_tag="ACHGNW_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN6926598.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="ACHGNW_002331"
     CDS             complement(7974..8441)
                     /locus_tag="ACHGNW_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN6926599.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2355 a         1814 c         1422 g         2983 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZBTH010000105         8494 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000105 DAZBTH010000000
VERSION     DAZBTH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.107"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHGNW_002332"
     CDS             complement(<1..149)
                     /locus_tag="ACHGNW_002332"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926600.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHGNW_002333"
     CDS             complement(593..1714)
                     /locus_tag="ACHGNW_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFN6926601.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHGNW_002334"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHGNW_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFN6926602.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHGNW_002335"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHGNW_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFN6926603.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHGNW_002336"
     CDS             complement(4304..5215)
                     /locus_tag="ACHGNW_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6926604.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHGNW_002337"
     CDS             complement(5232..6239)
                     /locus_tag="ACHGNW_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN6926605.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHGNW_002338"
     CDS             complement(6236..8365)
                     /locus_tag="ACHGNW_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6926606.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHGNW_002339"
     CDS             complement(8370..8480)
                     /locus_tag="ACHGNW_002339"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926607.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZBTH010000106         8339 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000106 DAZBTH010000000
VERSION     DAZBTH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.108"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..464)
                     /locus_tag="ACHGNW_002340"
     CDS             complement(<1..464)
                     /locus_tag="ACHGNW_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN6926608.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLAN"
     gene            complement(594..1304)
                     /gene="budA"
                     /locus_tag="ACHGNW_002341"
     CDS             complement(594..1304)
                     /gene="budA"
                     /locus_tag="ACHGNW_002341"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN6926609.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1317..2981)
                     /gene="alsS"
                     /locus_tag="ACHGNW_002342"
     CDS             complement(1317..2981)
                     /gene="alsS"
                     /locus_tag="ACHGNW_002342"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN6926610.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3189..3851)
                     /locus_tag="ACHGNW_002343"
     CDS             complement(3189..3851)
                     /locus_tag="ACHGNW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6926611.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3861..4676)
                     /locus_tag="ACHGNW_002344"
     CDS             complement(3861..4676)
                     /locus_tag="ACHGNW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6926612.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4937..5380)
                     /locus_tag="ACHGNW_002345"
     CDS             complement(4937..5380)
                     /locus_tag="ACHGNW_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFN6926613.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5514..5852)
                     /locus_tag="ACHGNW_002346"
                     /pseudo
     CDS             complement(5514..5852)
                     /locus_tag="ACHGNW_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5840..6217)
                     /locus_tag="ACHGNW_002347"
     CDS             complement(5840..6217)
                     /locus_tag="ACHGNW_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6926614.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6441..7634
                     /locus_tag="ACHGNW_002348"
     CDS             6441..7634
                     /locus_tag="ACHGNW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6926615.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7800..8222)
                     /locus_tag="ACHGNW_002349"
     CDS             complement(7800..8222)
                     /locus_tag="ACHGNW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926616.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2446 a         1688 c         1411 g         2794 t
ORIGIN      
        1 aaatttgcta ataaaagatc tacatatttt tctagaaatg cttgatcgat ttcatcatac
       61 cctttcgtta ctccgcaatc gatatctaaa acaccgatta aattaccttc ttttaccatt
      121 ggcactacga tttctgattg tgcggctgaa tcgcaggaaa tgtaatttgc atgggttttg
      181 acatcatcaa cgatcaatgt ttcttgtttt tcagctgatt ctccacaaac tccttttccc
      241 atcttgatac gtgtgcaaga aactcgccct tggaacggtc ctaaaatcaa ttcattacca
      301 tcgaataaat aatatcctgc aaagacagtc tcaggcaaag tctcattcaa caatgcagat
      361 gaatttgcta aatttgctaa ccagttatct tctgcttcta ataatccttg ttgctgtaat
      421 aataaaattt cgtatgcatc ttttttctgt ttttctgtcc acatgataaa aaccgctcct
      481 ttttgatcta tggagaagat tatagctcag tttttttgta aacgctaggt ttcctcctca
      541 aaaaaacgag atacgtcagg ttaatcacct tgaacgcatc tcgctgtctt tttctattct
      601 gcttgttgga tttctgctgc taaatcagca taatcaatcg tttcgtgaac aaaagtcgga
      661 tcatcaactg gaaaatgctg tacaagggac gagatgcggc taatctcaac ttttccttca
      721 gccacttcaa aatccattac atgtccgcca aatgtatgtg tatcatcgat aaaatgcagg
      781 tgaaatcccg ctgcagcaac tccttggaat aattcaggcg taaagaaccc gactaatgtt
      841 ccttggatat tgctttgttg gaattctggt tgagactctg aaatttttgc tagtcttctg
      901 taaggttttt cttgcttcgg catgatacgg atgtgcatat ttttgaataa tccagttatc
      961 ttcactgcca aaaataggtt ttccccggct tctcttacta atatgtcttt tttcaattgt
     1021 tctccatctg cagtttttga gacagaaaaa ctagtatctg gcgtaaaatc tgtaattgct
     1081 gcataggggg taagttcact tccatctaat tgggcaactg agccatctga tcttccttga
     1141 taagctacgc catctaaaaa gatgacttca ccatcaagcc catgcaatgt tcccaatccc
     1201 agagttcctt tttctaatat atgagcaatc gtttctgttc catccatcaa gccggccatc
     1261 aaagctccta atgtcccgtg ctggtataag attttttcag tcatgtcgac acttccttaa
     1321 tagaattgat ctggtaagat tgtttctccc aatttctcat tatcacggta atcaatcgga
     1381 atatcaataa ttacgggtcc ttctgtcgca aatccttctt ctaaagcttt ttctaattcc
     1441 tctacacttg tagctcggat tcctttagcg ccaaaagctt ctgcatattt cacaaaatca
     1501 actggaccaa agtctacacc tgacgcccgc tgatatttca ttttttcttg gaactctacc
     1561 atattgtagt gtccatcatt ccaaatcaaa tgaacgatat tcaatttttt acgtaccgcc
     1621 gtttctaaat cctgggcaga gaataaaaat cccccatcgc cggaaactga tacgatttgt
     1681 gtatttggac gcaccaatgc agcagaaatc gcccatggca atgcaacacc aagcgtttgc
     1741 ataccattac tgaataacaa atgacgaggt tcataacttc taaagtggcg tgccatccaa
     1801 atataatgac tacctacatc tacggtaaca gtcatatcat ctgttacttt tgactgaagc
     1861 gtgttaataa cttctaaagg atgcaagata cctgcttctc cttttgattg aacaatgtcc
     1921 cgctccgtta attttgcttg aagagatgct aaatattctt ttgcatcttc tgacacttgt
     1981 tgaggctcta atgaacctgt caataaatca agagtggctg agatatcacc gatcaattct
     2041 ctttcaggct gcataaaagg atcgatttca gctggcgctt catcgatcac aatgatccgc
     2101 gcatcttttt ccgcattcca gtttcttgct tcatattcga ttggatcata gccaattgca
     2161 ataactagat cacttcgttt caaaagcata tctcctggtt ggtttctaaa taaacccaca
     2221 cgaccaaaga aatgcgcttc taattcacgt gagatcactc cagctgcttg gaatgtctct
     2281 acaacaggca atgctgtctt ttcaactaat tgcctgatgg ctaacgtttc tttttcactt
     2341 gatcctcgca tacctactaa tagtaccggt agttttgctt ctctaatgcg ttcagctagg
     2401 taacggatat catctgcaga agcagaacct aatttaggat cgctcaatgg cttgatgaca
     2461 ttaccttgga caggtgcatc cactacatct tgcgggatac tgataaagct cgcgcctttt
     2521 tttgaactct tagccatacg ataagcattg gcaatcactt ctgaaattgt ttctggatct
     2581 tggatttctg cactgtattt tgtgattggc tggaacagtg ctgcattatc catgctttga
     2641 tgggtcaact tcaatagatc gcttcgtttt acttgtccgg caattgctaa aactgggtca
     2701 ccttctgctg tcgctgttac caaacctgta gctaagttac tagcaccagg accacttgtt
     2761 gcgataacta ctcctggctc tcctgtaata cgtccgattg cttgtgccat aaatgcagca
     2821 ttttgttcgt gacgagtcac gattaattca ggaccttgat cctctaattc attaaataca
     2881 ccatcgattt ttgcacctgg tataccgaat acataatcaa catgatgatt aatcaaactt
     2941 tctactacag atttcgaacc ttgtttcatt ttttcagcca ttgaacatcg tcctttttat
     3001 gctttatttt taattattag ttagaaataa tcacaaataa gtgatacagt ttctaaacct
     3061 gtttcataca aaagtatagc acgttttatt tcatttgttt agccatatgt ttcaaaaaat
     3121 gaaaaagaat gacagttttt acataaaaaa aactgtcatt cttaattgaa agtttcaatt
     3181 tataaagtct acagatccat agttctcatc aatttacgat tgttgaagta aatcaaacca
     3241 aatacgatag aaggacctag tagaatacac cattggggct ttacgaaaaa gaagagaaac
     3301 aaacttgtca atagtaaaaa atctagtaaa ctcacatacc attttttcaa cgtatagtag
     3361 aacgctatcc gtagaacttg atttggtttc gacgctggat ttctcacctg aaaataccag
     3421 cagttggtgc taaaggaaac tgataaacaa gcaagtaaaa caaacaacgg aatcaaccat
     3481 tttccataag aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac actaactatc
     3541 caagcaaata gccaataaaa aaatcccttt agccaaaatt ggcgaaacat ctttatgaac
     3601 aaacgtactg gtgaaaaact attttctttt gctaattctt ctgtcatacc tatcatcacg
     3661 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
     3721 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
     3781 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
     3841 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
     3901 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
     3961 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
     4021 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
     4081 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
     4141 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
     4201 atactactgt aatattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
     4261 accaacgacc acaacgtatc tgttaaaccc acttttctca tcataatgaa ctgcgggatc
     4321 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
     4381 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
     4441 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
     4501 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
     4561 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
     4621 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     4681 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     4741 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     4801 tcttattttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt ttttgattta
     4861 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     4921 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     4981 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     5041 ggtcaagcgg aacgaatgtc actcgctttg aatgatgact tacagcacct tctatgacta
     5101 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     5161 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     5221 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     5281 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     5341 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     5401 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     5461 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ctatcagctt
     5521 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     5581 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     5641 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttactct
     5701 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     5761 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     5821 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     5881 catatttctt gatttttcct tgtaaacgtt ccaaacattc ttgcatttca gcaatttttt
     5941 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     6001 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     6061 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     6121 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     6181 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     6241 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     6301 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     6361 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     6421 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattagta tcgacatcgg
     6481 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     6541 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     6601 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     6661 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     6721 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatatg
     6781 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     6841 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     6901 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     6961 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     7021 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     7081 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctac
     7141 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     7201 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     7261 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     7321 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     7381 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     7441 cgacggcgaa taatttcgtt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     7501 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     7561 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     7621 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtgga aaaagcttgc
     7681 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     7741 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     7801 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     7861 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     7921 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     7981 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     8041 atgaccttat caggatattg ttggaacaat tcttctaaat ctgttagata attcaagata
     8101 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     8161 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     8221 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa cggatacaaa
     8281 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttcct
//
LOCUS       DAZBTH010000107         8209 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000107 DAZBTH010000000
VERSION     DAZBTH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.109"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHGNW_002350"
     CDS             476..1339
                     /locus_tag="ACHGNW_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6926617.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHGNW_002351"
     CDS             complement(1468..3090)
                     /locus_tag="ACHGNW_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6926618.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHGNW_002352"
     CDS             3339..3860
                     /locus_tag="ACHGNW_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6926619.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGNW_002353"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGNW_002353"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN6926620.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHGNW_002354"
     CDS             5941..6759
                     /locus_tag="ACHGNW_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN6926621.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHGNW_002355"
     CDS             complement(6862..7059)
                     /locus_tag="ACHGNW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926622.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHGNW_002356"
     CDS             7228..7806
                     /locus_tag="ACHGNW_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6926623.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHGNW_002357"
     CDS             7901..8038
                     /locus_tag="ACHGNW_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926624.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2460 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaata
//
LOCUS       DAZBTH010000108         8208 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000108 DAZBTH010000000
VERSION     DAZBTH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.110"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACHGNW_002358"
     CDS             94..1248
                     /locus_tag="ACHGNW_002358"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN6926625.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACHGNW_002359"
     CDS             complement(1270..2022)
                     /locus_tag="ACHGNW_002359"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6926626.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACHGNW_002360"
     CDS             complement(2386..5052)
                     /locus_tag="ACHGNW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6926627.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACHGNW_002361"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACHGNW_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6926628.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHGNW_002362"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHGNW_002362"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN6926629.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2388 a         1701 c         1458 g         2661 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttca
//
LOCUS       DAZBTH010000109         8114 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000109 DAZBTH010000000
VERSION     DAZBTH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.111"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACHGNW_002363"
     CDS             387..575
                     /locus_tag="ACHGNW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN6926630.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACHGNW_002364"
     CDS             653..1666
                     /locus_tag="ACHGNW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926631.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACHGNW_002365"
     CDS             complement(1733..2767)
                     /locus_tag="ACHGNW_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6926632.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACHGNW_002366"
     CDS             2882..5107
                     /locus_tag="ACHGNW_002366"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6926633.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACHGNW_002367"
     CDS             5125..7008
                     /locus_tag="ACHGNW_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN6926634.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACHGNW_002368"
     CDS             6989..7963
                     /locus_tag="ACHGNW_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN6926635.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2621 a         1476 c         1710 g         2307 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgggg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg aacaagagag
     8041 taaaaaagtt ctcagatact tttgttcatg actattttct tcaataaaac caattgttat
     8101 ttttctgaaa aaca
//
LOCUS       DAZBTH010000110         8105 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000110 DAZBTH010000000
VERSION     DAZBTH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.112"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..891
                     /locus_tag="ACHGNW_002369"
     CDS             190..891
                     /locus_tag="ACHGNW_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6926636.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1500..2516)
                     /gene="tsaD"
                     /locus_tag="ACHGNW_002370"
     CDS             complement(1500..2516)
                     /gene="tsaD"
                     /locus_tag="ACHGNW_002370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN6926637.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2513..2977)
                     /gene="rimI"
                     /locus_tag="ACHGNW_002371"
     CDS             complement(2513..2977)
                     /gene="rimI"
                     /locus_tag="ACHGNW_002371"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6926638.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2984..3526)
                     /gene="rimI"
                     /locus_tag="ACHGNW_002372"
     CDS             complement(2984..3526)
                     /gene="rimI"
                     /locus_tag="ACHGNW_002372"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6926639.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3510..4232)
                     /gene="tsaB"
                     /locus_tag="ACHGNW_002373"
     CDS             complement(3510..4232)
                     /gene="tsaB"
                     /locus_tag="ACHGNW_002373"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN6926640.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4423..5403)
                     /locus_tag="ACHGNW_002374"
     CDS             complement(4423..5403)
                     /locus_tag="ACHGNW_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6926641.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5427..6911)
                     /gene="galT"
                     /locus_tag="ACHGNW_002375"
     CDS             complement(5427..6911)
                     /gene="galT"
                     /locus_tag="ACHGNW_002375"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN6926642.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6923..7912)
                     /gene="galE"
                     /locus_tag="ACHGNW_002376"
     CDS             complement(6923..7912)
                     /gene="galE"
                     /locus_tag="ACHGNW_002376"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN6926643.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2299 a         1724 c         1419 g         2663 t
ORIGIN      
        1 gttgtttttt atacacaaat cattcgcttc aaaacaaatt attcaatacc attgtatatt
       61 ttttacataa taacaaatta tatttgtaaa gtattaaaac ttgtttctaa tgttggtatg
      121 ttaaaacttt acaaattctt taaaaaaagt gacaataaat atagtgaaca ataaggagag
      181 gactttttaa tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct
      241 gattttgaac atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt
      301 tcttataaaa aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc
      361 ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat
      421 gactatgtga atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac
      481 acttatacag ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat
      541 gaaaaattag cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa
      601 gtagttgaaa tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg
      661 gtaaaacaaa atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat
      721 atcaccattc cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt
      781 gaaactgctg gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa
      841 aagcgtttta ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa
      901 aaataagcag tgaccctgat aaatatcagg gtcactgctt attttcctcc ctaaaacttc
      961 tgaaagatcc ggcagtttta gatcactgga caagactcaa agattttcta tgtgtgtttc
     1021 agttatgtaa tattcactgt ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt
     1081 gtcctcatcc gatctagatt attcgatatt cctcgacaat aaacgattac tcagtgaggg
     1141 caatcagagg aacggataat gataaataag ctgtaaaaaa tagcaaaata acaataatga
     1201 ttaaaagtat gtaaaaaata gggctttgac aaaaacgtgt cacagcccta tttcgattaa
     1261 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1321 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1381 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1441 agccgttttt cctgggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     1501 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1561 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1621 gggggaatca ctagttctac ctcagggagt ttagctgcca aagcatcctg caatccttca
     1681 cgcaaccctt gattggccgc tacacctcca gctataatca gttgcttgac tggataattt
     1741 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1801 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1861 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgc
     1921 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1981 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     2041 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     2101 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     2161 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2221 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2281 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2341 gtgatttgtt ctacgtgatg gcggctagct acttcaggta ctacgccacc aaaacgttta
     2401 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2461 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2521 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2581 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2641 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2701 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2761 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2821 cttcagctaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2881 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2941 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     3001 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     3061 ttgcttgaaa ccccaatttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     3121 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3181 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3241 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3301 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3361 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3421 attctcttag tacgtaacga cgatcgattt gttcgactgt tttttctgta taaatcattt
     3481 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3541 ggtgtgtgcg tctccaacca tttttcttct gcttctactc gttttaaata gcgaggaaga
     3601 aaagcttgtt tctctgcaac tggttcaact aaagcaccta attgagccac agttacacca
     3661 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3721 tcagagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3781 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacagt ttctaatcta
     3841 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3901 acaataatat tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3961 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     4021 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     4081 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     4141 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4201 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4261 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4321 gtacaataat acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa
     4381 aagctgcggt atcactccat tttcccacag cttttccatc ttttatttcg ttgattctcg
     4441 aataatcaac tctgtttcca caataatctt tcttgaaacc ggtgctttgg aattacttaa
     4501 ttgaagaatc gtctccactg ctagctctcc catccattcg gtataaactt ttacagtaga
     4561 aagcgagggc gttacatatt tcgctacact gacatcattg aagcccatca cagaaatatc
     4621 ttctggtacc actaaacctt tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc
     4681 attagctgca aaaagcgcat caggaacatg cggattcgtt tttaaatatt gcttcactgc
     4741 ttgatatcct gactcgacag aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt
     4801 ttctgccaaa acttgttgaa atgccataat tctgggatct tttagccgct gatggttttt
     4861 cttcgtatat tctgttccag caagcatagc gatatgttgc gctttatggg agacaaagtg
     4921 ttcaatgact ttttgaacac ttgacttaaa gtcgacaact agagagtcga ttccttgaga
     4981 agtgccgtca gaatctacca aaagcaaggc attttgggat cctttcattt ctaataactg
     5041 agcttcgtcg aattttccta gtgcgatcgt tccatctgcc ggaagatcac taatggaatc
     5101 ccacgtttct ttcaataaag taacgtttgc ttcttctgcc tttttttcga ttcctaggcg
     5161 aatagataaa taataaagat cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg
     5221 aagcgtttgc ttagtatttg ctgcattttt acgatgtttc gtataattta agcgttctgc
     5281 tgcttcaaaa attttttgct ttgtggctaa gccaacggaa agatcaggat catagttgag
     5341 aactcttgaa acagtagcag gagaaactcc tgccaactgc gcaatgtctt taattgttgc
     5401 catttgtctc tcctcctttt ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc
     5461 tgtccttttt catctcgctt gaatactcca gcgtcttcca gtacacgtgc aaaaacctga
     5521 cctacagatt tttgtacgac atcggtgacc gtatctttcg tcaaatcagg atgttctttt
     5581 tttaattgtt ctgcccacgg acgatgatat tcagccatct ggttttcttc tcctaataag
     5641 aaatgctcta cttctactag ttctggtttc aaacgaggtg gtaaaacagc aagccccatt
     5701 acttcgatca acccaatgtt ctcctttttg atatgttgga catctttatg tggatgaaaa
     5761 atcccatcag gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag
     5821 taattgtccc ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc atctgctgaa
     5881 aaagctctga tagatactgt ttcatccgaa tatgctttcc atttttccaa tactgagtta
     5941 gctgcttcaa taagcaagtt tttctctgtt gattgcaaac ggatcacaga catcggccat
     6001 ttcacgactc ctgcgatgac ttgcggataa tcttctagtt tgataagttt ttctatcggt
     6061 gcttttgcca taggaaattc atgacgtcct gcctgataat gatcatgaga aagaatcgat
     6121 cctccaacaa ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa gacttcagta
     6181 atccgaagca gtctagcaaa tgtttccttt gtgattttca tcgggcggtg ttgttctgat
     6241 aaaatgatcg aatgttcatt gtaataagca taaggggaat attggaatcc ccagctttca
     6301 ccgtctagat tcattcgaat aatccggtga ttggtgcggg ctggatagtt caagcgacct
     6361 ctatatcctt cgttttccat gcaaagcatg caggtaggat agtctacttt taccgcctcg
     6421 cgttctgctg cgatttgttt tggatctttt tctggcttag ataaattgat tgtgatttcc
     6481 agttcaccat actccgactc tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg
     6541 atgtagtcat ttttcttact taaattaaag aaatactcag ttgcttcctc tgggcttttc
     6601 gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag aaaatccatt
     6661 aattgcgctt caaaaatctc ttgttctgct ggcaattctg aaagaacacc attttctctt
     6721 gccttagcga caagctgatc caataagtct acagattctt ccttaactgg tcttacttca
     6781 atgctttcta gagcatcttc tcctaccatc cctaaaagac gattctgtaa atataagcgg
     6841 tccatttcca tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga
     6901 ctgattgaca tatctcattc ccctattctt cataaccatt tggatggctg acatgccaat
     6961 cccatgctgt tttgataatc gctttgatat ctgtgtattc aggtttccag cccagaatct
     7021 ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc tccagctctt cttggtgcta
     7081 tttctgctgg aatttctttt cctgtcactt ctctagctgc ttctagcatt tccttgactg
     7141 agtatccttt gctgctgcct aaattgaaga agttgctttc atttccttct ttcaagtatt
     7201 ccaaagctaa aatatgtgca gcaatcaaat cttctacttg aacataatct ctgatacatg
     7261 ttccatctgg tgtatcataa tcgtctccat aaacagccaa ggcttttctt tgaccaagag
     7321 ctacttgtaa aatgattggg acaagatgcg tttctggtgt atgatcttct ccgatagatg
     7381 catctgcttt tgctccggct acattgaagt agcgcaaagc gacatagcgc atgccatatg
     7441 cttggtcaca ccacttcatc attttttcca tcatcaattt actttcacca tacggatttt
     7501 taggattagc tggtgtatct tctgtgattg gagactcttt cggctctcca taagttgctg
     7561 cagtagaaga gaatacgatt ttattcacgt tgaactcgtg catgacttca agtaagattt
     7621 gcataccgta tacgttgttg ttgaaataca tcaatggttt ttcgacagat tctcctacta
     7681 aagagcttgc agcaaaatgg attactcctt caatcggttc cttttcaaat acggaacgta
     7741 gaaattcttt atcccggatg tctccttcat aaaaatgagc atctggatgt actgcttgtt
     7801 tgtgacctgt gagcaaatta tctaccacag ctacttgata ccccttttga actagctggt
     7861 ctacagcgtg cgaaccgata taaccggctc cgcctagtac taaaattgtc ataaaaaaca
     7921 ctcctttgat tagtaaaatt gtgtttgttg attctatata atttaaaaaa cgaagtttac
     7981 ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc atatggtaaa tttataataa
     8041 aacatttagt aaacattttc aagggttttt tgcaaaggtt tacaaacaaa cgtaaaacta
     8101 gcctt
//
LOCUS       DAZBTH010000111         7868 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000111 DAZBTH010000000
VERSION     DAZBTH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.113"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACHGNW_002377"
     CDS             complement(21..995)
                     /locus_tag="ACHGNW_002377"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6926644.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACHGNW_002378"
     CDS             complement(1075..1950)
                     /locus_tag="ACHGNW_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN6926645.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACHGNW_002379"
     CDS             2080..3987
                     /locus_tag="ACHGNW_002379"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN6926646.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACHGNW_002380"
     CDS             complement(4528..7806)
                     /locus_tag="ACHGNW_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN6926647.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2218 a         1662 c         1462 g         2526 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tattttta
//
LOCUS       DAZBTH010000112         7618 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000112 DAZBTH010000000
VERSION     DAZBTH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.114"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..790)
                     /locus_tag="ACHGNW_002381"
     CDS             complement(62..790)
                     /locus_tag="ACHGNW_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6926648.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="ACHGNW_002382"
     CDS             complement(803..2083)
                     /locus_tag="ACHGNW_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6926649.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="ACHGNW_002383"
     CDS             complement(2224..2826)
                     /locus_tag="ACHGNW_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6926650.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="ACHGNW_002384"
     CDS             3071..3277
                     /locus_tag="ACHGNW_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926651.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="ACHGNW_002385"
     CDS             3589..4308
                     /locus_tag="ACHGNW_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6926652.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="ACHGNW_002386"
     CDS             complement(4389..5072)
                     /locus_tag="ACHGNW_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN6926653.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="ACHGNW_002387"
     CDS             5290..7476
                     /locus_tag="ACHGNW_002387"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN6926654.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2730 a         1283 c         1435 g         2170 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggt
//
LOCUS       DAZBTH010000113         7600 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000113 DAZBTH010000000
VERSION     DAZBTH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.115"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHGNW_002388"
     CDS             complement(138..428)
                     /locus_tag="ACHGNW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926655.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHGNW_002389"
     CDS             complement(546..806)
                     /locus_tag="ACHGNW_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926656.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHGNW_002390"
     CDS             complement(943..1356)
                     /locus_tag="ACHGNW_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN6926657.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHGNW_002391"
     CDS             complement(1812..2297)
                     /locus_tag="ACHGNW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926658.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHGNW_002392"
     CDS             complement(2436..2651)
                     /locus_tag="ACHGNW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN6926659.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHGNW_002393"
     CDS             2875..3675
                     /locus_tag="ACHGNW_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6926660.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHGNW_002394"
     CDS             3694..5562
                     /locus_tag="ACHGNW_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6926661.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHGNW_002395"
     CDS             5555..6046
                     /locus_tag="ACHGNW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN6926662.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHGNW_002396"
     CDS             6034..6588
                     /locus_tag="ACHGNW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN6926663.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACHGNW_002397"
     CDS             6606..>7600
                     /locus_tag="ACHGNW_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN6926664.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZBTH010000114         7499 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000114 DAZBTH010000000
VERSION     DAZBTH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.116"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1163
                     /locus_tag="ACHGNW_002398"
     CDS             135..1163
                     /locus_tag="ACHGNW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926665.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1232..2572)
                     /locus_tag="ACHGNW_002399"
     CDS             complement(1232..2572)
                     /locus_tag="ACHGNW_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6926666.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(2731..3171)
                     /locus_tag="ACHGNW_002400"
     CDS             complement(2731..3171)
                     /locus_tag="ACHGNW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6926667.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3331..3837
                     /locus_tag="ACHGNW_002401"
     CDS             3331..3837
                     /locus_tag="ACHGNW_002401"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6926668.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4005..5918
                     /locus_tag="ACHGNW_002402"
     CDS             4005..5918
                     /locus_tag="ACHGNW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6926669.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5923..6924
                     /locus_tag="ACHGNW_002403"
     CDS             5923..6924
                     /locus_tag="ACHGNW_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6926670.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7126..7332)
                     /locus_tag="ACHGNW_002404"
     CDS             complement(7126..7332)
                     /locus_tag="ACHGNW_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN6926671.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2616 a         1207 c         1535 g         2141 t
ORIGIN      
        1 taagtttgcg tacgattcaa agaaaattaa gagaacttaa tactattttg aaaaactatc
       61 atttatcttg cagcttgaaa aaaggtaatc cgatagaagg agaagaatac cgtatccgat
      121 acttttttca cttgatgtat tggcagtcat tcgacgaaaa tcattctgat atattatcaa
      181 gtataaaaaa taaacgtcaa ctattagaaa aatttgcgaa acatgcgcca tatacgcgta
      241 gtatagatag aaagaaatgg atcagcctac taagaatttc gttgcaacga atgaaacaag
      301 gccactcgat tacgtatgtc ccagaagaga tgaacaaatt tgtgcatcca ttgataacaa
      361 aagaagagtt tacgcattac atactagagc ctttttttga agcaaacttc tggtcgatga
      421 atcacatcac tccaacagag caagcttacc tttattttat gtttggtgta atgaacacct
      481 atttggaaga agatttgttg gatactccag ataaagtaca agaaagttgg ggctggattg
      541 aatcgtctat tgtagaaaat tttgaaaaaa acttcaagat ttgttttaca acttcagaaa
      601 aaaattatct atatgtcaat ttggcaatga tccatttgta ttcaagggtt tttgggacaa
      661 aaaagaaggt ggatgctttt ggaaaatcag cagaagacag agattttcag catatatttc
      721 cagtgatgta tccaatcatg caacagtttt ttagtggggt agctcgcaga attccagagc
      781 ttaatactta ttatgaaaag aacagacggt tgatttttca atactgtatg ttagtcaggg
      841 atatttttat aaaacacgaa caacctgtca tattctatat tcaatcaaag tatggtaaaa
      901 cacaggaatt atgggcaaag aaaaggatat tatctatgac tgaacgccca ataaaatata
      961 gtgcatccgc tgatcagtta cctgatattg tcattagcga ttatcctatc gacaaaaagg
     1021 catatgatca aacgaataca cagatatttt actggaatgg acaacctaca aaaaatgatt
     1081 gggcgcgtct gaaagctgaa atcataaaaa tcaggaatga gaaaaataga tccatattgg
     1141 aaaaattgga tttcgttaat taagaaaagc ggctcttcgt caatgagtgt tggtgaagaa
     1201 attcaataga ataatcgaga tactcacacg gctagacagc tatcttgtct agccgtgttg
     1261 ttgttctggc tttagtcagt tgggtagtgt tcttagtttt cttgaaatat tttcctcgat
     1321 ataaaaagat tcgttctcgg tagttttgaa agtttctaaa gccataggca attcgtttta
     1381 gtactttgat atggttgttc aaacattcta tagggccgtt ggaataggga actcgcaacg
     1441 ctcgtttgat ttctgttcga tatttcttga aggtcgtgaa cactggttga tagtgctctg
     1501 gcaactcctt gtagtcttct tttaggagcg cgacgaattc aggaacatct tgtcttctga
     1561 tagcggaaag aaaaacctgg tagacttcgt agcctcgctt taaggagtca tcataatata
     1621 atagccgatc gactatctcc gcttctgtca gatgatctcg aaatgacgga cgccagtagc
     1681 gatgctgacc attcaattcc cacgcattct tttgaagtag tctccagtat ttctttaagt
     1741 gcttcgaccg acgatcccct tttttaaagg tcttcattac ttgaattcgg tggttggtaa
     1801 aggcccgacc gatgtgttgg ctaatatgaa agcgatcggc gacgattcgg gcattcggaa
     1861 aacactcttt caccaatgtg cgatagggtg tatagaaatc tgaaaccact agacgtactt
     1921 gttcacggac ttttctaggg tagcgcaaga aatagtcgcg taaaggattg agttgccgat
     1981 tttccacaat atcaatcagt tgatgggtct tgccgtccat catgacaaag ctcatggctc
     2041 ccgagacgtt tttcaccgat ttgaattcat cgaaacacac cacttctggt aacggctggt
     2101 tcaaagaaac ggttttaggc cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa
     2161 tgtgcttcat acgtgcaatc aaagacatag aaataggctc acttaatagc tcaagaatgg
     2221 cttgtttgac tctgcgggca atggagcagc gacgatcgct aactgaatct tctgctaaaa
     2281 aagtctttcc acagtgttta catttgaagc gctgtttgtt aatccgcaca atggtcttgt
     2341 agttactgac atcattcaat aagatggata cttttttcca gccccattta atgatcgttt
     2401 gcttattttg atagtggcaa tggaagcatt cctctggttg ataggtcaat gtgccatcaa
     2461 aaacaaaaca tgtttcgtca ttaatgcgct ctttgcgaaa ggcatcttca tgaaaggtaa
     2521 gatttaggtc tagaatatct agtaactctt tggtaaactc tgaataggac atcttgaaac
     2581 actcctttga atggttttcg tcgactttat tctaaaggat ttcaaggtgt ctgtttgcgt
     2641 ttattagaaa aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag
     2701 atccagaaaa gcagattgtt tatgccaaaa ttaagacatt gcaaaaagat tattttcttt
     2761 aaaatcgaca agatgaccag aaagcaagag ttcgttgagc tcttgctttc ttttagtttt
     2821 agaaaggaac atcctgattt tatcttcgct aaatccagtc aaaagttttg cagattggtc
     2881 gtagataatc ggttttttca ataaagaagg ggatgtacga atctcaatat atagttcact
     2941 caaactgagt tcatctattc tgtgaacaag atttttataa ctaatagaat gagtagagag
     3001 catttcggtc acgccgttgt ttgtcaattt tagtaagtgg ataaattcat ttttggttag
     3061 tggttgacga attaagttct gttcgataaa cggtatatca tggacgataa gccaacgttt
     3121 tgcgcgtctt gaagacgagc agccagaacc tgtatacaat ttaatcatca tacattcctc
     3181 caatagctaa gtatttatga aataataaca tgaagaaata gtcgaaaagt gccatttaga
     3241 ggtgtaaaat aagaaaatgt tgtcacgtat aatggaacta gctagataag aaatactatt
     3301 aaatcaagaa aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc aatagtagtg
     3361 atcgatttac aaaaaggggt atgttttgat tcttcgccga tacacgaatt agaaacagtg
     3421 acagcattag tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc agttattttc
     3481 gttcaacacg aagatgctca attagtgcca caaacaaaac catgggagct attgtcggaa
     3541 ttatctattc ctgagcacag ttattttgtg agaaaaaccc atgcgaattc attttataag
     3601 actaatttga gtgttttatt aactgaattg gactgtcatt cgatcgaatt atgtggggca
     3661 caagcagagt attgtataga tacgactgtg aagtttgctc atggattagg ataccagctt
     3721 atgatgtgta gaggagcaac gacaacttat gataatcctt ttatgaaagc aaaggaaaca
     3781 atcgcttttt acgagaaaat ttgggataaa cgatttctga catttttgga gcaataagtt
     3841 gtttcttcgt ttaaaaaaag cagaatggat actggtttat ccattctgct ttattacgta
     3901 aaatgagaaa ataatcctgt tctattttta tgggataaac catttatttt ttgtataaaa
     3961 aatgtttgcg tttacatata attctcctag aaacataaaa aggagtgaat agcttgagaa
     4021 caaaacaagt attaaaggga gcgttggtca tcttatttgc tgtaagtaca agcgtcccgc
     4081 aatggacagt atatggagag agcatttctt ccgaatcgga atctcgggag gtagaaacca
     4141 cagaaactac tgatgctgga aatgaaacga aatcgagtac ttcacaaaca gaaacaaata
     4201 ctgaaacaac ggttgagaca tctgttacta gcacagatca agaaacgacc gctaccacag
     4261 ataaccaaca aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa tcctcttctg
     4321 ctaaagtaag tgacaaagag aatgggacag aaaatgatga tatcggtaaa ggtgaaaatt
     4381 tcaatggtaa agaatatttg aattacttcg gtaaacagat gacagaaata cttaatggac
     4441 caacagaaga catcaagtca aaattaatag agttattagt cactgagctt gctgttaatg
     4501 attatggtgt agataaagag attatcaaac atctgattgc tacttatcta ttgtacgata
     4561 tcgcttttgc aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat gatgggacaa
     4621 cgcctacaga aaacacaggc ttctatccaa atacaaaaca acttcatcaa actgtactaa
     4681 acaaacaaga tggcaaaatg atggatgtat atgcatatta tgtcgatcaa ggttcagata
     4741 aaacagtcat gatccatggc ggatttcgag ggaattggaa taacgggatc gttacagaag
     4801 aatacaatga tttttataag gccgggtata atttattatt tgttgattcc agagcgactg
     4861 gtaatagcgg aggcgattat gtgacctatg gacaatatga gtcagatgac gtcctttact
     4921 ggataaatca agaagttcga gagcggccat ctcaaaagat tttactttat ggtggttcga
     4981 tgggagcagc tacgatgatg tctgtattag cgaaagatat tcctgtcaat gtcaaaggaa
     5041 ttattgaaaa ttgcggattt gcatcaattg atgaacaact acgttttacg tacagccaga
     5101 cagtagcccc cgcactaccg gatgccataa aaaatcagtt agacatcatt ggggatcaag
     5161 agcatgaaga tttatttatg ggccttttga aacaatacta ttttgatcag gagatgcatt
     5221 tggatacaaa agctgctcta cctacgattg gaatgtcaga ttcattgcct aaattgatta
     5281 tccatggaac agcagatgat gtagtacctg tgtctaacgc gcaaaaatta tatgagttat
     5341 caggagggta taaagatctt ttattagtag aaggagcagg gcatggaaag gcacaggaag
     5401 ttgaccatgc tgcctacacg aaacatgtaa cggacttttt gaaagtagtg ttccatgatc
     5461 agataaacgt aaagtatgtg gatgaaaaca ataaaagtct actcaaagac caggacgaaa
     5521 tccagcttta cggagcttat ggagaaaatt atatgacaga gcaaaagaca ttcgaaggat
     5581 acgaattagc aaatgttgaa ggaccgactg aagggatttt taatgagacg ataccaacta
     5641 ttattttcta ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat ccgacagaga
     5701 atgcagacaa taagggaaag aatgaacctc gagaggatca atcaagtgat gatacgcaac
     5761 cgattcagat gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga atgttgccga
     5821 aaactggaga aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca
     5881 cgattatcac atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga
     5941 atagtgattg cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat
     6001 caaatcacac caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa
     6061 gaacaagtat atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa
     6121 aaagaaatat cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa
     6181 gtggaaaaga aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg
     6241 gataaaaatg attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc
     6301 gtaaatatag attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg
     6361 aacgaagcca ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt
     6421 tcgattggtg gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa
     6481 aatgaagaaa ttaaaaaagc tgatcatctt gatccgactt tggagaacaa gcagattacg
     6541 aagtttattt ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa
     6601 atctctagct ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa
     6661 caatcagaga aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac
     6721 acattcttaa cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct
     6781 gtatttgaga aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt
     6841 ttgaaccatg aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag
     6901 ttagtaatgt ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact
     6961 tagctccaag tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg
     7021 taacttgtga ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat
     7081 atcacacgat tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt
     7141 tgatcgttta aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc
     7201 gagtggtaag caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg
     7261 ggaacaaaga tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg
     7321 gatagctttc ataagcagca cctccttttt tataattgta tgataaatag attaagaatc
     7381 tataaatgtg acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca
     7441 aagatggcta atgataagca attaatgatt cagcaatcag ataggtacaa agtttacca
//
LOCUS       DAZBTH010000115         7467 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000115 DAZBTH010000000
VERSION     DAZBTH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.117"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..2227
                     /gene="acm"
                     /locus_tag="ACHGNW_002405"
     CDS             62..2227
                     /gene="acm"
                     /locus_tag="ACHGNW_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN6926672.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2499..2984)
                     /locus_tag="ACHGNW_002406"
     CDS             complement(2499..2984)
                     /locus_tag="ACHGNW_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN6926673.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3074..3419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3464..4105
                     /locus_tag="ACHGNW_002407"
     CDS             3464..4105
                     /locus_tag="ACHGNW_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6926674.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4124..4759
                     /locus_tag="ACHGNW_002408"
     CDS             4124..4759
                     /locus_tag="ACHGNW_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6926675.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4777..5592
                     /locus_tag="ACHGNW_002409"
     CDS             4777..5592
                     /locus_tag="ACHGNW_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6926676.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5911..7347
                     /locus_tag="ACHGNW_002410"
     CDS             5911..7347
                     /locus_tag="ACHGNW_002410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6926677.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2617 a         1206 c         1565 g         2079 t
ORIGIN      
        1 ggatttttga gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa
       61 aatgaaaaag tgtagaaata ttattttatc aatgttattt attattacta atataacatc
      121 actgattcct gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc
      181 gttgaccgtg gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt
      241 tgacgaaaaa aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga
      301 aggaaatatt cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa
      361 tgttggaact ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa
      421 aaacctagac tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga
      481 taccggtgaa gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt
      541 aaatgtgaca aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga
      601 catgctacct gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac
      661 ttatgttgaa caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc
      721 aagtacgttt gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga
      781 gggaatccaa caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga
      841 taattacatt gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag
      901 ttataagacg atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg
      961 gtttaaagaa tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa
     1021 tatttcagct tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata
     1081 tatcaatgac acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga
     1141 acagcctatt caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag
     1201 tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat
     1261 tgattttgat ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga
     1321 gggagtcagt ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat
     1381 ttggaacggt ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac
     1441 aaacaattgg gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc
     1501 agtgacttgg gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt
     1561 gcaagaagta gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa
     1621 cgggctagtc gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg
     1681 ggaagacaat gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc
     1741 cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt
     1801 cacaaatctg ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa
     1861 cgtgcccgaa tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga
     1921 aaaaacaaat atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa
     1981 gacagagaga gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac
     2041 aggagaatcc agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat
     2101 aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt
     2161 aatcattggt ttattgatag taactatctg tgctgggata ttcttacaaa aatataaaaa
     2221 agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc
     2281 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
     2341 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
     2401 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
     2461 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
     2521 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
     2581 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
     2641 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
     2701 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
     2761 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
     2821 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
     2881 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
     2941 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
     3001 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
     3061 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
     3121 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
     3181 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
     3241 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     3301 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     3361 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     3421 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     3481 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     3541 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     3601 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     3661 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     3721 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     3781 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     3841 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     3901 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     3961 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     4021 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     4081 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     4141 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     4201 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     4261 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     4321 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     4381 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     4441 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     4501 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     4561 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     4621 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     4681 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     4741 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     4801 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     4861 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     4921 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     4981 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     5041 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     5101 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     5161 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     5221 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     5281 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     5341 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     5401 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     5461 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     5521 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     5581 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     5641 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     5701 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     5761 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     5821 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     5881 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     5941 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     6001 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     6061 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     6121 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     6181 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     6241 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     6301 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     6361 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     6421 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     6481 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     6541 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     6601 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     6661 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     6721 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     6781 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     6841 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     6901 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     6961 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     7021 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     7081 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     7141 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     7201 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     7261 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     7321 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     7381 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     7441 tgaaaaatga aagaaagcgt cagttct
//
LOCUS       DAZBTH010000116         7422 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000116 DAZBTH010000000
VERSION     DAZBTH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.118"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHGNW_002411"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHGNW_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN6926678.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHGNW_002412"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHGNW_002412"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN6926679.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHGNW_002413"
     CDS             complement(2303..2965)
                     /locus_tag="ACHGNW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6926680.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHGNW_002414"
     CDS             3149..4795
                     /locus_tag="ACHGNW_002414"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6926681.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002415"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHGNW_002415"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6926682.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7422)
                     /locus_tag="ACHGNW_002416"
                     /pseudo
     CDS             complement(5976..7422)
                     /locus_tag="ACHGNW_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2326 a         1363 c         1371 g         2362 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat at
//
LOCUS       DAZBTH010000117         7347 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000117 DAZBTH010000000
VERSION     DAZBTH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.119"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..307)
                     /locus_tag="ACHGNW_002417"
     CDS             complement(<1..307)
                     /locus_tag="ACHGNW_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN6926683.1"
                     /translation="MNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLGGKVYYAPRLY
                     PQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRIAHSHNTSIDR
                     "
     gene            complement(367..1503)
                     /locus_tag="ACHGNW_002418"
     CDS             complement(367..1503)
                     /locus_tag="ACHGNW_002418"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6926684.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1535..2986)
                     /locus_tag="ACHGNW_002419"
     CDS             complement(1535..2986)
                     /locus_tag="ACHGNW_002419"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6926685.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3030..3794)
                     /locus_tag="ACHGNW_002420"
     CDS             complement(3030..3794)
                     /locus_tag="ACHGNW_002420"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN6926686.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3822..4520)
                     /locus_tag="ACHGNW_002421"
     CDS             complement(3822..4520)
                     /locus_tag="ACHGNW_002421"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN6926687.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4532..5311)
                     /locus_tag="ACHGNW_002422"
     CDS             complement(4532..5311)
                     /locus_tag="ACHGNW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN6926688.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5327..6271)
                     /locus_tag="ACHGNW_002423"
     CDS             complement(5327..6271)
                     /locus_tag="ACHGNW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6926689.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6317..7096)
                     /locus_tag="ACHGNW_002424"
     CDS             complement(6317..7096)
                     /locus_tag="ACHGNW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926690.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2034 a         1566 c         1144 g         2603 t
ORIGIN      
        1 ctctatctat cgatgtatta tggctatgtg ctatccgtat cggaatccca gctttttttg
       61 cagcttttaa aggtaaataa ctcatcgcat caatatgaga atgaacaatc tcatattctg
      121 gatgatcctt aaaaaattgt tccatccatt taaaatattt aggataattt tgtggataaa
      181 gtctcggtgc ataatagacc tttcctccaa gacttattat ttcctcatca taatcacttt
      241 tatatggacg atgtgtcaaa aaatcaaact gaattttagt tcgatctata ttcctataat
      301 agttcattag catcgtctcc aaccctgcac gatgcatatc attcacacac tgtaatattc
      361 gaatcattat atatttcccc tatacaaatt taatgtttct tgtacgattt tatcccaatt
      421 atatttacta caaataaact cagtagcttg attcttcaaa actttaacca tttccgattg
      481 attgcatgcc tcttctaatc ttatctttaa atcacttaca tccgatttct taaaaatcat
      541 agccttgtct tctactactt ccgtacactc tgagatattt gaaactatac aacagttccc
      601 ataactcatt gcttctaaca aacttaatgg cattccttct aaatcacttg gaagtgtata
      661 gatatacgca ttactatata attcttctaa ttcctgtcct tgtacaaatc cagtaaatat
      721 aattcgctca tcaccttttg ctaattcctt tagttcattt gcaaattcat ctgtatcaga
      781 gcttcctccg gctattataa gttttttatc tgtctgaaca tctttaaatg cctcaattag
      841 ataccgaatt cctttttcgg gaaccaatcg ccccaaaaac aaaatataag aatcttttgt
      901 taatccatac ttttctgtaa tcaatcctgc tgattttatt tcaatttttt taacgccatt
      961 tggtataaac cttgtttttc ttccatatat atcttcaaaa taattttgga cgctttcact
     1021 aagtacaatt atttcatctg caaattttac agcacacttt tcccctaaca tgatgtatgt
     1081 actagcaagc ttgttccatt tttctctttg atgatctaga ccatgaattg ttgcaacaca
     1141 tcttttccca aaaagttttg gcaaccacaa catagcacat ggtccttctg catgaaaatg
     1201 aacaacatca tattttccga atgctgctct aattccacca aatactgaag aactcattgc
     1261 tgcaattcct tttatattta atgtaaaaat agtctttaat tttacacctt tatattcttt
     1321 atttttatgc tggtcaaatt cctttccgct aacatgatga ccacttcgat tatagcaagt
     1381 cacttcatgc ccaagtgcaa ccattcgcgt tgccaattct tctaccacta tctcaattcc
     1441 accttctctt gatggaattc ttttatgacc tagcatacag atttttaatt tttttcttgc
     1501 catcacgttt tactccttct atagtatctt tatcttacat ggaaccgttt ctatgaagca
     1561 cagttttgac tgtcctaaac aaaattctaa aatctaatcc catactccaa ttcctgatat
     1621 attctgcatc aagttttaca acctcttcga agtctgtgat atcacttcta ccactcacct
     1681 gccacattcc agtaatccca ggtttcatcg caattctcgc acgatgatga aaatcatact
     1741 tctcatattc tgacttcaag caaggtctaa accccaccag gctcattttt cccattagga
     1801 cattataaaa ttgtggaaac tcatccaatg atgtagatcg aataaattgt ccaattcctg
     1861 tcttctttgt tccatcagga agtattttat tgccaatgat tcttgggtca aaatccatct
     1921 taaacatgag attattactc atcttatttt ctttcatcaa ctctgctttg cgttcttctg
     1981 catccatata catactacgg aacttataca tcttaaatgt cttaccattt tgaccaatac
     2041 gttcttgtgc aaagaaaatc ggccctggtg atttgatgta aataactggt gctacgaaga
     2101 taaaaataac acctgttgca atacatccaa taattccagc aagaatgtca atcgttctct
     2161 ttataaatgc ttgcctcatc gtcataacat taatactaga tgtaataaca gtataatcac
     2221 caactcggcc aataaattgt tttccacgtg tactatcaga cacttttgta agattcatat
     2281 gtacagcaag tcccatttcc gaacatctgg cgattaattc tttgggatat ggaacacttt
     2341 cgtcaacatt gatgaatact tcatctaccc aattctgtaa aagatattcc gctgcattat
     2401 cagcatttgc aactactggg acacctccga tggtagttcc atacatatct ctgtctacta
     2461 caatcacacc actaagttta tacatgccat aattcttctc tttaagatta cgaactacat
     2521 tttttgcaat cttttgagat gttacaataa ctaacgatac atcttcaccg tttttcatgc
     2581 ggtgatgcaa caaccctttc caaagtatac gaattatata tgttaataat ccatacacca
     2641 cccccattat aaataaagaa actctggaat aatcatttcc atcctgcatt gtaataaggt
     2701 ataaactgca cacaagtaac agcataaaag attgctttat agaagctaca gcctctttat
     2761 aatatcctct ttttaaaaca tttttatatg cctcaaataa aaatataatc gtaagattct
     2821 caagttctat aaaaattgcc atgtggcgat aaataggaat cacatacgga ttccagtcac
     2881 cctgtcgcat tacatatgat atataaaaag caagctggac acaaaataag tctaataaaa
     2941 taaaatcaat atgtttagac caaccacttg agttttttct atacatctct cactctctcc
     3001 tcatttcctt tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc
     3061 tcttcataga ttggataatt gatctcatcc ccgttgatca gatcctttgc gacttgtttc
     3121 atctgtacta ctttttcctt accaaactcc ttggcaatct gctcatagca ctcttttaag
     3181 taaaaggtgc gcttagagac cccgtgtgca tcagaagcag ccatctgcac gagattatgt
     3241 ttgaccattg ttttggctgt tttctggata tctttgccaa atacaccaac ataacttggc
     3301 gcagtaagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttct
     3361 cgaaaatgcg cgttgcgttc tggatggacg atcactggta tctttcctag ttctaacaac
     3421 tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     3481 tatgtatcat ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt
     3541 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat
     3601 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     3661 ttattgtggt gtggtgtgca taaaatatgc gtaatgcctt gctgaatcgc tttttcagcc
     3721 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     3781 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     3841 ccagcatctt tgctgtgttc tgctccgttg tatacgacac ctaagactcg tgcttgtacc
     3901 atatctaaca gttcttttgc tttagtcaat gattcttttc gtgacgtatt ttcccgtacg
     3961 accaataagg tgccatccgt ttttgaagac atgatctgtg catctgttac ggcaacgact
     4021 ggcggcatgt cgaagatcac tacatcaaat agttgtcttg cttctgctaa aatctgatcc
     4081 attcttggcg aacttagtag ttctgatgga tttggtggtt taggtccact tggcagaatc
     4141 gacaaattgt cgactggtgt tcgttggatc tcatctgcta cacttcctga tgaactaaga
     4201 acagtactta atccgcttgc gttgttcaac ttgaacgttt tatagatgac gggcttacgt
     4261 aagtctgcat cgactagcag cacccgttga ccagatttgg caaaaacaac ggcaatgttt
     4321 gctgcagtgg tcgatttccc ttctccaggt cctgatgaag tcacaacaat cgttttgatt
     4381 tgctgaccag ctgcggaggc aaactggata ttcgtccgaa tcgtacgata ttgttcagcg
     4441 attggtgaag agggattggt gagtgtgacc aaactgactg catgagtttg ggctgctttt
     4501 tgtttctgtg ttcgtgccat tttctttcct cctagactct tgagcggctt ctgcgtgatg
     4561 gcgctgtttt atcgtttgta gcttttggtt gtgtctgagc tggtcctttt ttacggatac
     4621 cagcattcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     4681 tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca
     4741 gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg
     4801 gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca
     4861 tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     4921 ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa
     4981 ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac
     5041 gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca
     5101 tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga
     5161 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     5221 tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa
     5281 aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     5341 gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt
     5401 gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac
     5461 caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt
     5521 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca
     5581 atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac
     5641 catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa
     5701 ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     5761 taccaccaac tgcatcaacg agttccttta gccctttcat attgatagag acatagtgat
     5821 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     5881 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatcac
     5941 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg
     6001 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa
     6061 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     6121 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     6181 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt
     6241 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     6301 atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctca gcaacagttt
     6361 gtgcttttgt tgcgtatgct gcgcttattt tttcattccg gatctgctct atttgactga
     6421 gcaccgtcaa atattgatcg taagtagcgg tggcagtcac ttgaccatcc tttaacatcg
     6481 tatcgaataa ctgatctagt tgcgtgactt gcgcgatctg aatatcagcg aatcctagat
     6541 attgcataac aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac
     6601 gagcgacatc tgcacttgtc accttttctt tgatgatgac atcgtctaca gcttgctgcc
     6661 agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc agttttgatt
     6721 ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt
     6781 taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca gaggatagct
     6841 tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa attttttctt
     6901 ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg
     6961 tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt
     7021 atccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga
     7081 cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt aagatataat
     7141 tcacttacat aaaaatgaca gctttttatt taaaaaaaca taattaaact aatatattca
     7201 cagaaatatt tgtaaacaag ttttccctat ataatcatac cacaaactgt tttcttgact
     7261 agggcaattt acgatttaaa agtaaataaa acattaataa gataaaaatt aatcgatttc
     7321 atttttattt ccataatttt cataggt
//
LOCUS       DAZBTH010000118         7316 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000118 DAZBTH010000000
VERSION     DAZBTH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.120"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..2712
                     /gene="clpB"
                     /locus_tag="ACHGNW_002425"
     CDS             103..2712
                     /gene="clpB"
                     /locus_tag="ACHGNW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN6926691.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2863..3549)
                     /locus_tag="ACHGNW_002426"
     CDS             complement(2863..3549)
                     /locus_tag="ACHGNW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926692.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3527..4021)
                     /locus_tag="ACHGNW_002427"
     CDS             complement(3527..4021)
                     /locus_tag="ACHGNW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6926693.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4041..5261)
                     /locus_tag="ACHGNW_002428"
     CDS             complement(4041..5261)
                     /locus_tag="ACHGNW_002428"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6926694.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5408..7243
                     /gene="lepA"
                     /locus_tag="ACHGNW_002429"
     CDS             5408..7243
                     /gene="lepA"
                     /locus_tag="ACHGNW_002429"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN6926695.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2406 a         1399 c         1502 g         2009 t
ORIGIN      
        1 ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa
       61 agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg
      121 actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat
      181 caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca ttttggtcga
      241 aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta
      301 ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag
      361 aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt
      421 ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt
      481 tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga
      541 ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac
      601 ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat
      661 gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg
      721 ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta
      781 cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca
      841 ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa
      901 gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta
      961 ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct
     1021 cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa
     1081 aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa
     1141 gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac
     1201 atccatgaca acgcattagt agcagcagct actttatctg accgttatat tactgatcgg
     1261 ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa
     1321 atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc
     1381 gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg
     1441 caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca
     1501 gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac
     1561 gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt
     1621 acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag
     1681 attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta
     1741 acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat
     1801 gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg
     1861 gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc
     1921 ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt
     1981 gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg
     2041 tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg
     2101 gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat
     2161 cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa
     2221 gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag
     2281 ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc
     2341 aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt
     2401 ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa
     2461 ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg
     2521 tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc
     2581 actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa
     2641 tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa
     2701 gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg
     2761 tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc
     2821 gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt
     2881 ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa
     2941 tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc
     3001 tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa
     3061 gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc
     3121 aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa
     3181 gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat
     3241 tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat
     3301 gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt
     3361 caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt
     3421 gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac
     3481 gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag
     3541 tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt
     3601 ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag
     3661 ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag
     3721 tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc
     3781 cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt
     3841 ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt
     3901 cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta
     3961 actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca
     4021 tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg
     4081 atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa
     4141 taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg
     4201 gattttccta taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg
     4261 aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct
     4321 gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa
     4381 tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt
     4441 ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat
     4501 aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca
     4561 gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct
     4621 gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat
     4681 aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca
     4741 actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg
     4801 attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata
     4861 ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg
     4921 ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc
     4981 acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt
     5041 tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt
     5101 tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca
     5161 gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc
     5221 ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt
     5281 agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa
     5341 actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg
     5401 agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat
     5461 catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca
     5521 cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga
     5581 acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga
     5641 aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc
     5701 tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga
     5761 agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt
     5821 catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga
     5881 cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat
     5941 tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc
     6001 accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa
     6061 tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg
     6121 caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt
     6181 tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg
     6241 agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg
     6301 aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga
     6361 tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga
     6421 aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga
     6481 tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc
     6541 cgttatttac catgttaata aaacggatgg tacgactgct acagtagata acccggcaga
     6601 ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat
     6661 ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt
     6721 cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc
     6781 tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt
     6841 ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa
     6901 tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg
     6961 aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat
     7021 ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa
     7081 cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca
     7141 aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt
     7201 catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa
     7261 acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtg
//
LOCUS       DAZBTH010000119         7130 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000119 DAZBTH010000000
VERSION     DAZBTH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.121"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACHGNW_002430"
     CDS             complement(1778..2443)
                     /locus_tag="ACHGNW_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926696.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACHGNW_002431"
     CDS             2569..4605
                     /locus_tag="ACHGNW_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN6926697.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACHGNW_002432"
     CDS             4609..5247
                     /locus_tag="ACHGNW_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926698.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACHGNW_002433"
     CDS             5275..5703
                     /locus_tag="ACHGNW_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926699.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..7071
                     /locus_tag="ACHGNW_002434"
     CDS             6160..7071
                     /locus_tag="ACHGNW_002434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6926700.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2452 a         1071 c         1311 g         2296 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     7081 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt
//
LOCUS       DAZBTH010000120         6749 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000120 DAZBTH010000000
VERSION     DAZBTH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.123"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHGNW_002435"
     CDS             complement(<1..182)
                     /locus_tag="ACHGNW_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6926701.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHGNW_002436"
     CDS             517..951
                     /locus_tag="ACHGNW_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6926702.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHGNW_002437"
     CDS             974..1294
                     /locus_tag="ACHGNW_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926703.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHGNW_002438"
     CDS             1435..1626
                     /locus_tag="ACHGNW_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926704.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHGNW_002439"
     CDS             complement(1697..1954)
                     /locus_tag="ACHGNW_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN6926705.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHGNW_002440"
     CDS             2112..2375
                     /locus_tag="ACHGNW_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926706.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHGNW_002441"
     CDS             2399..2587
                     /locus_tag="ACHGNW_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926707.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHGNW_002442"
     CDS             complement(2670..2855)
                     /locus_tag="ACHGNW_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926708.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHGNW_002443"
     CDS             3070..3441
                     /locus_tag="ACHGNW_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6926709.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHGNW_002444"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHGNW_002444"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHGNW_002445"
     CDS             complement(3709..4137)
                     /locus_tag="ACHGNW_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926710.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHGNW_002446"
     CDS             complement(4197..4955)
                     /locus_tag="ACHGNW_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN6926711.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHGNW_002447"
     CDS             5250..5447
                     /locus_tag="ACHGNW_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926712.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHGNW_002448"
     CDS             5553..6035
                     /locus_tag="ACHGNW_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926713.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHGNW_002449"
     CDS             complement(6093..6689)
                     /locus_tag="ACHGNW_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6926714.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2277 a         1043 c         1333 g         2096 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagta
//
LOCUS       DAZBTH010000121         6644 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000121 DAZBTH010000000
VERSION     DAZBTH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.124"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHGNW_002450"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHGNW_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGNW_002451"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGNW_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN6926715.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHGNW_002452"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHGNW_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHGNW_002453"
     CDS             complement(2143..2373)
                     /locus_tag="ACHGNW_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN6926716.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHGNW_002454"
     CDS             complement(2603..3421)
                     /locus_tag="ACHGNW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6926717.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHGNW_002455"
     CDS             3582..4271
                     /locus_tag="ACHGNW_002455"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6926718.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHGNW_002456"
     CDS             4285..5787
                     /locus_tag="ACHGNW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6926719.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHGNW_002457"
     CDS             5800..6276
                     /locus_tag="ACHGNW_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6926720.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHGNW_002458"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHGNW_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZBTH010000122         6615 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000122 DAZBTH010000000
VERSION     DAZBTH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.125"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..2806
                     /locus_tag="ACHGNW_002459"
     CDS             131..2806
                     /locus_tag="ACHGNW_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN6926721.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2988..3227
                     /locus_tag="ACHGNW_002460"
     CDS             2988..3227
                     /locus_tag="ACHGNW_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926722.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3243..4616
                     /locus_tag="ACHGNW_002461"
     CDS             3243..4616
                     /locus_tag="ACHGNW_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926723.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4620..6254
                     /locus_tag="ACHGNW_002462"
     CDS             4620..6254
                     /locus_tag="ACHGNW_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926724.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6417..>6615
                     /locus_tag="ACHGNW_002463"
     CDS             6417..>6615
                     /locus_tag="ACHGNW_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926725.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2318 a         1068 c         1329 g         1900 t
ORIGIN      
        1 acaacttttt tcttttgtca agattttgag tgggatatat ttttgtagaa ctgaagatca
       61 tgaaattatg agaggttgga ggtaatgtag tcatacaaat aaaaattttt tagaaagtga
      121 gtggtaatta atgaaaatga atcacacttc aattcagaag catttacaac tgttaaatac
      181 actggggatt tcttatgtta atttgagaac tttttctaca ttaaacaaga aaaccgtttc
      241 tcatattttt tctactgcag attctcaagc aattattaac tatttagaaa aacagaaatt
      301 gtctgaaggt acagtaaaca taacttataa tcctattaaa caagaggtga ttacacaaaa
      361 aaagcattct gtgagagaca atgatattga aaaaattcgg tttgtattta ttgatattga
      421 ccctaaaagg aaagagggaa gtgcaacaga tagcgaaaag aaaaaggcag aaaatgttat
      481 ggaacaagta gaacgttatt tgaaagaaaa gagaatagaa tcatttgtaa aagttgatag
      541 tgggaatggc taccatattt ttcttcccat aaatgaacaa ccaaataatc acgagacgat
      601 tcttactatt cagaattttc ttcaattact tcaccgaaga tttggagtag aagatgaagt
      661 tgatattgat acttctgttt ataatccttc aagattatgc aaattagttg gtacaccagc
      721 ggtcaaagga aagtccacaa aagagcgtcc gcatcgttta tctagtttcc tttctattcc
      781 cgataacata gatagaagta attgttttaa aattcttgaa cagatagttg aagaaaattc
      841 catgatgtca acctctagag taaaaagcca tccatctttt agagaagtgt taccttttat
      901 tcaagcagac tataaacaat ggttagctca ctatcctgaa ttaacttata ccataaaaga
      961 gggcgatcat aaaggagtta ccttacttat atttgacgaa tgtccattaa gaaaacacac
     1021 gaataattct aatggttctt gtttgtctgt tacggctgat aataagattc gatttcattg
     1081 tctgcatgct tcggataaaa ataaagatat tcaagacttt gtaaaaaaat atccgttgcc
     1141 aaaacctgta aatccttctg tagaatcatt agaatccaat caagaatatt attttgacca
     1201 gtttaaactg aatcaagaag gagtattttt ggtgaaagaa ggtcaagaag aaaaagttgc
     1261 ttgtccgatg tttattaagc aaatacgcaa aaatatcgaa caaaaagtca ttacacttgt
     1321 tcttgcctat tatgtcgatc attgttggga tgaaatagaa atagaaggtg gatgtttaca
     1381 aactaataat tttaaacaat tagcaaaaca tggcttaatc attaaagcta gaagagaagc
     1441 tgaagtcgtt gactttttac agattcaacg ggagaatgct ccaatctttt ttgaacatga
     1501 atctcttggc tggaaaaaag aaaatgaagc aatgattttt cagttacaag attcttttgg
     1561 acgtgaaatt ccttctgtat taactaaaaa ctcatgctac caattaacaa ctaatggtac
     1621 agaagcggaa tttgacgcat tggttcataa gtatgtttta ggaacgaact tagaacttgc
     1681 ttggtcgatt ggtattgctt ctattgtgtt aggctattta aacgttacga aggctaatac
     1741 ttttttgagt ttactaatta acctacaggg caagagcaca acgggtaaaa ccactacgtt
     1801 acactttatt gcctctttat ttggaaatcc aactacggtt cttagaaata tgaatgctac
     1861 caataaagcc attataaagc tagcatcaaa caatcaggga ggtgttccac taatactaga
     1921 tgagttagga gcagcaactc aaaataactt tagctcgttg atctatcaat tagcttcagg
     1981 agaagaaagg ctaagattag attctaattg tcagcttatg ccacaagaga ctttttctgt
     2041 tttgaccctt acctcttctg aagagcgttt ggaaaattat ttcaatgaga aattaactgg
     2101 tttgaaagtt cgatatatcg aattttctga tatcaagttt acaaaggatg cgaatcacgc
     2161 agagaaagtt aaaagtcttt cttctaatac ttttggttgg ggtataaagt tatttctttt
     2221 gaaactcttt gaacaaggga ttacaaaaat tgaagcaact tttgtcgaag ctcaaaaaaa
     2281 actatgggct gttttacctg aacattcttt gaagcaacgc attggtaata atctagcttt
     2341 gatttatagt ggagccctcc taattcaaga tatactgggc tatcctttac gattagagca
     2401 tatacagacg gtgcttatta attcgtacga acagttaatt gtagatacta gtttaggaag
     2461 tgagcaaacg gtatttgacc gagtgaaaac attgttggtg atgaatagcc ataaattttt
     2521 aacaaacagt gacaacaaac atacgcccag tacgatttgg ggaaaagtat caattcaaaa
     2581 aaatggggat attaaggtaa atatttttcc tatcccgttt gaggacttgt taaggcgaga
     2641 atttcaagta aaggatctaa cctatctttt taggcaattg cttgattcgg gtaatatgtg
     2701 tgcagaaaaa gggagaagaa cgaaacgtat tcacatgaat cgtcaagttc ttccaacata
     2761 cgagattcta ttaccttctt cactcaaatc gtattttcga ttgtaattta gaataaacca
     2821 aagtatgaaa gtgaggcacc atgttatgga aatatttctt attgatttag caaaacaact
     2881 gattacttat ctgtatgata gcgagcaaac ccaaacggaa gtaaatgaag cttgatctat
     2941 gcttcattta cttcattcaa tcaaacatga aagaaaggaa aattctaatg actaaaaata
     3001 aaatgacact aaaagcagag gttcttctgt atattcaaga acatttttca aatcaagcct
     3061 tttttacaaa accaatttac ttggatttcg aaataagagg ggtatctgct ggatctatcg
     3121 ggggtactct tcaagcgttg aagaacgaag gatatttaga aaatcacttt gtgcaacgtt
     3181 cgttcaatag aagagtcgtg aaaaaatggt atttagttca ttcatagaga ggggaatcaa
     3241 gcatgcgtgt ggcttgttat gatcgaacgt ctgtttatga agaagcgaaa attagtaatg
     3301 acgctcaata tgatgcagtg aaagagtatg ttttagagaa atatgaacaa attccccaac
     3361 gattttcaga caaaggagaa tcaggggaaa aagcggagcg tccagctttt cgacagctta
     3421 gagcttgggt ttgtgcgtca gaagaagagg gggtgatcta tgtttggcgt tatgatcgat
     3481 tggcacgtca agccaaaata gccttagaat ttattgagtt gtgccaacaa catcaggttc
     3541 aagtgtattc gatttctgaa cctcttcctg cttctttctc acacgcgtca acgaataaac
     3601 tatttgtgca attattgttc acttttgcag aacaacaacg aaacgtcatt attgagaatg
     3661 ttcgtgccgg actttcctat aaaaagcaac aacggcaata tttgtcttct gcggtaccat
     3721 atgggtatcg tttagtgaat gggaaaatcc aacaagaagt acacgaagca aggattgttc
     3781 gctatatttt tcaactttat ttgaccaaaa aatatggcta caaaaagctt tgtcaacgat
     3841 tgacgcaaca aaagcttttc ttccgcgaac gtccttttca gccctatcat atttatagta
     3901 tcctaaaaaa tccattgtac tatggcgaaa tcaaaggagg atctttgggg aagtatctag
     3961 gaacctttga accgattctc tcgaaagcaa cctttttaca agcccaagag attcgccaat
     4021 caaggtgtac agctaaaaaa gatacgtatc tgtacttatt acgtcaaaaa attcggtgtc
     4081 ccttctgtgg tcgtcatctg agttcgaagt atcaatggaa tacaaagaag acgaagacgc
     4141 ttcattatta tcattgtacc gaccgaagct gccaaggaat ttttcttcgg gcagaaaggg
     4201 ttgagtcaga agtattggaa aagatacagg ctttttttca atacgaaggc gtctatcaag
     4261 ccttgattgc ccaattaacg caacaattag ccgaaaagcg aaaagatgaa aaacgcaaag
     4321 taatccaaca aaagaaacaa gaagcacaat ggattaaggc atttgaagag ggcaattgtt
     4381 cactggaaga actcaagcaa caactgcaag ctcttcaaaa gccgatgtct acttcttcgt
     4441 ttctttcact ccaacagtgt gaagcaaaag tgaaagaatg gcttcaacta aaagacctct
     4501 ctagtcgaca attattatgt gactatgttt gtcggattga ggtaacaaag gataaaacag
     4561 tttgtgggat ttacttccga caactgacac aaaatatttt agaaaggaac gaatgatcta
     4621 tggaaatgat acaaaccaaa cgagtcgcat tgtatattcg cgtgagtaca caagaacaag
     4681 ctttagaagg acatagtatt caagcacaag aacaaaatgg acgacagttt gccgaacgga
     4741 tgggctatga agtggtagaa gtttatgccg atgaaggaat cagtgggaaa tccacaaaaa
     4801 atcggttcgc ctatcaacga atgatgaacg atgctcggaa ccatcgtttt gatctactag
     4861 tgatttggaa attgacccgt ctcggacgca atatgttaga tgtcttacaa acagtcgagg
     4921 aattattgcg atatgacgta ggattacatt cgatctcgga acaatttgac gtgaccactt
     4981 cgacaggtaa attgatgtta caactccttg ggagtttcgg tgaatttgaa cgcaaccaaa
     5041 ttgcggagaa tgttcaaatg tcaatgcaaa gcttagtgaa agataaaaag cgttatgcag
     5101 gaggccgacg tttaggctat gtcagtggta ttgacacgaa tggtcaaaaa caattggtca
     5161 ttgaaccaga agaggcgaaa attgttcgtc tgatctatgc aaaatattta ggcggagcag
     5221 ggtatcgagc gattgccaat tttttgaatc gtcaaggata tcaaacagtc aaaaagaatg
     5281 ctttttctac cacggcagtc aaagatatct tacgcaataa aatttatgca gggtatttag
     5341 aatatgcgcg ctacatcaat tgggaaacca aacgacggaa ggggaagaat ccgagaccga
     5401 ttttagtgaa aggaacccat gaaccgatta tttcggaaga agattatcaa aaagtacaag
     5461 aacgccttgc tttagaagga aaacaaccca aatggaacca tacaggtgaa aatgtattga
     5521 caggcttgtt acgttgtcca gaatgtggcg caccgatggc agcgagtaat gtcacaaata
     5581 ccctaaagga tggcacaaag aaacggattc gatattattc ttgttcagtc tttcggaata
     5641 aaggagcaag tgtgtgtcat gccaattcga ttcgtgccga acaagcggaa acatttgtcg
     5701 ccgaccgatt aaaggagatc gttcagctac cagaagttct tccccgattg gtggcagcgt
     5761 taaatgaaga aatagtgcga caaagccaac cattggaaca agaactcgtg gtgttactgg
     5821 aacgaaagga agaattgaag actaaaattg aaaaatggga agctgctttg gaagatagtc
     5881 ctgaattgtt tccgatgtta aaagaccgct tagacgaatt gactgaaaaa cgacgacagt
     5941 tgcacattcg agaaaatgaa atcttaggga tctttcaaca aggagagccg attcaagtca
     6001 aagatgtcca acggatattg actaatttgg atcgttttct tgcccagagt gagaagaagc
     6061 aaatcaaagc cttgtatcgt acctttatag agaaaataac ctttgatcct aaccataagg
     6121 agatgttgga aattacgatg aaattcacgc atgccgtggt acaacaattg aatgagcaat
     6181 atcaaatagc cgtatccaaa acaaaggata cggctatttt tgtgttaaaa acacccttta
     6241 cgttaacgat ctagtctagg ttatatcaaa caacgttaga ggggcgctaa cgcttctcta
     6301 gcgatttgaa atcgttagag aagtagttag ggaggtcctt atcataaata gggatattta
     6361 ttaatgaata tttgaaatat aattgtatat aagaattaaa aagagtgggg atagatatga
     6421 aaaagatggg taagtgtatt ttatgtgaaa aatacactga acttactaaa gaacatgtac
     6481 cacctaaaaa atcagggaat actggaggta aaaagaccag aactggtaat ttagatgatt
     6541 tccttaagag cgattttaca aaaggtgatt ttccgaaggg gattaaaagg aaacctcaag
     6601 gaaatgttta ttata
//
LOCUS       DAZBTH010000123         6570 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000123 DAZBTH010000000
VERSION     DAZBTH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.126"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHGNW_002464"
     CDS             127..579
                     /locus_tag="ACHGNW_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926726.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHGNW_002465"
     CDS             593..1282
                     /locus_tag="ACHGNW_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926727.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHGNW_002466"
     CDS             1407..3758
                     /locus_tag="ACHGNW_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN6926728.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHGNW_002467"
     CDS             3838..4233
                     /locus_tag="ACHGNW_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926729.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHGNW_002468"
     CDS             4558..5175
                     /locus_tag="ACHGNW_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926730.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHGNW_002469"
     CDS             5216..5515
                     /locus_tag="ACHGNW_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFN6926731.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHGNW_002470"
     CDS             5549..5716
                     /locus_tag="ACHGNW_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN6926732.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHGNW_002471"
     CDS             complement(5761..6366)
                     /locus_tag="ACHGNW_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926733.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZBTH010000124         6529 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000124 DAZBTH010000000
VERSION     DAZBTH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.127"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..771
                     /locus_tag="ACHGNW_002472"
     CDS             34..771
                     /locus_tag="ACHGNW_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6926734.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            785..1600
                     /locus_tag="ACHGNW_002473"
     CDS             785..1600
                     /locus_tag="ACHGNW_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6926735.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1613..2260
                     /locus_tag="ACHGNW_002474"
     CDS             1613..2260
                     /locus_tag="ACHGNW_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6926736.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2276..2932
                     /locus_tag="ACHGNW_002475"
     CDS             2276..2932
                     /locus_tag="ACHGNW_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6926737.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3094..3915
                     /gene="racE"
                     /locus_tag="ACHGNW_002476"
     CDS             3094..3915
                     /gene="racE"
                     /locus_tag="ACHGNW_002476"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN6926738.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3918..5273
                     /gene="rph"
                     /locus_tag="ACHGNW_002477"
     CDS             3918..5273
                     /gene="rph"
                     /locus_tag="ACHGNW_002477"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN6926739.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5274..5792
                     /locus_tag="ACHGNW_002478"
     CDS             5274..5792
                     /locus_tag="ACHGNW_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6926740.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5899..6399
                     /gene="cbpB"
                     /locus_tag="ACHGNW_002479"
     CDS             5899..6399
                     /gene="cbpB"
                     /locus_tag="ACHGNW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN6926741.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2150 a         1097 c         1442 g         1840 t
ORIGIN      
        1 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
       61 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      121 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      181 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      241 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      301 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      361 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      421 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      481 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      541 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      601 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      661 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      721 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      781 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      841 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
      901 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
      961 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1021 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1081 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1141 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1201 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1261 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1321 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1381 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1441 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1501 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1561 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1621 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1681 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1741 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1801 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1861 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     1921 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     1981 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2041 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2101 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2161 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2221 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2281 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2341 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2401 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2461 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2521 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2581 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2641 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2701 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2761 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2821 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     2881 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     2941 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3001 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3061 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3121 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3181 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3241 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3301 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3361 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3421 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3481 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3541 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3601 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3661 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3721 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3781 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3841 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     3901 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     3961 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4021 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4081 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4141 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4201 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4261 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4321 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4381 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4441 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4501 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4561 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4621 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4681 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4741 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4801 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4861 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     4921 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     4981 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5041 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5101 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5161 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5221 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5281 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5341 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5401 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5461 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5521 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5581 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5641 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5701 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5761 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5821 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     5881 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     5941 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6001 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6061 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6121 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6181 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6241 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6301 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6361 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6421 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6481 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaatt
//
LOCUS       DAZBTH010000125         6326 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000125 DAZBTH010000000
VERSION     DAZBTH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.128"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..915)
                     /gene="nth"
                     /locus_tag="ACHGNW_002480"
     CDS             complement(238..915)
                     /gene="nth"
                     /locus_tag="ACHGNW_002480"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFN6926742.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(922..1620)
                     /locus_tag="ACHGNW_002481"
     CDS             complement(922..1620)
                     /locus_tag="ACHGNW_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6926743.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1888..3072
                     /locus_tag="ACHGNW_002482"
     CDS             1888..3072
                     /locus_tag="ACHGNW_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN6926744.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3078..3521)
                     /locus_tag="ACHGNW_002483"
     CDS             complement(3078..3521)
                     /locus_tag="ACHGNW_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN6926745.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3747..4034)
                     /locus_tag="ACHGNW_002484"
     CDS             complement(3747..4034)
                     /locus_tag="ACHGNW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6926746.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4205..5557)
                     /locus_tag="ACHGNW_002485"
     CDS             complement(4205..5557)
                     /locus_tag="ACHGNW_002485"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN6926747.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5830..6276)
                     /locus_tag="ACHGNW_002486"
     CDS             complement(5830..6276)
                     /locus_tag="ACHGNW_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6926748.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1698 a         1264 c         1055 g         2309 t
ORIGIN      
        1 tcctttggaa tactggactt attttatagg tatattggaa ggcattattt tctacatcgg
       61 gtattttccc aataaaaata acaactttta tttttatcgc aataaattgt aataagttac
      121 catcctagct actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc
      181 ttttctccat aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta
      241 caggcgtggt ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg
      301 acatattgat agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct
      361 accaaaaaaa atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat
      421 cagtgtttct tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga
      481 gacacgttcc acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac
      541 gacatttgct gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg
      601 cacttgccca tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt
      661 atttcgatac aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc
      721 taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac
      781 atctgtcgcc tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt
      841 gagttctcca tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt
      901 ttgtttgctt aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa
      961 ttcttcttgt tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt
     1021 ttggtcttct tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat
     1081 gcggtcaacg taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt
     1141 caaaatttcc ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat
     1201 cgaagaaagc gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt
     1261 ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc
     1321 atagatgggg taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat
     1381 cttgataaat ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat
     1441 tcccatatcc atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat
     1501 atgcaactgt agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt
     1561 aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat
     1621 gatttctcct ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt
     1681 tcttttctat ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt
     1741 tgttcatgtt tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc
     1801 tgttccttag aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt
     1861 aacattattg cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt
     1921 tttggatacg tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt
     1981 ccgtttttaa cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg
     2041 ctcatgtctg tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt
     2101 gatcattttg ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat
     2161 ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga
     2221 ttgacggcag gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat
     2281 gaaagaacca aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt
     2341 cctattatag ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca
     2401 ctctttactt tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata
     2461 aaaaaacgtt cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa
     2521 ttattcaaga tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca
     2581 gctggctctc ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct
     2641 gggttggttg gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc
     2701 attatgcctc gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc
     2761 agtgaaatcc tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg
     2821 tttttatttg ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat
     2881 ggccaactat ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa
     2941 agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca
     3001 gcaccatttc ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac
     3061 aataaaaaat agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa
     3121 cgaaatactg tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt
     3181 acccattttt gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat
     3241 tgaaaataga tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct
     3301 atcagttttt ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat
     3361 tttgtcacgt taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga
     3421 gtaaatcctg caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct
     3481 aaatccagat attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct
     3541 ttcattatat tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac
     3601 ccaatctgaa accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat
     3661 taaaagatag taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg
     3721 atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc
     3781 tgaccttcat atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt
     3841 ctactgaaag gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa
     3901 tattcctctt gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg
     3961 atcgttaatt tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa
     4021 tattctttat tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc
     4081 ttttatgtaa cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac
     4141 taattgtact tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt
     4201 ttctttattg tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat
     4261 tccatgggcg gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag
     4321 tcattttatt ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg
     4381 acatgaactg agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta
     4441 ctttttcagt agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga
     4501 cttctagatc gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc
     4561 caaataggta aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca
     4621 agttatttcc cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt
     4681 gcgtgccact tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat
     4741 tacttgtctg catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc
     4801 cttcgatcag ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa
     4861 atgtatctgt tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag
     4921 taacattttc gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat
     4981 ctgtgaatgg tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca
     5041 cttgttttcc agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa
     5101 caattactac acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt
     5161 ttttgcatag gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg
     5221 aaccagttgt catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat
     5281 cttttgccgt tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa
     5341 ctgttgctcc taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct
     5401 ttactactcc gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc
     5461 tttcatatac tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag
     5521 tacctgcatg agtacagcca attactacta ctttcattta tggtccctcc gattaacatt
     5581 ttgtccttcg acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt
     5641 gaattcacaa aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta
     5701 caagattctt attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa
     5761 aaataaaaaa gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag
     5821 aaactgtttt tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata
     5881 caattccaac cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat
     5941 atcttcaagc cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg
     6001 ataatgacgc tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg
     6061 aacaaatcca atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt
     6121 tggaaaccga tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc
     6181 aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa
     6241 cttttcgata acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct
     6301 cgtcttatca tactattttt acattt
//
LOCUS       DAZBTH010000126         6316 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000126 DAZBTH010000000
VERSION     DAZBTH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.129"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..715)
                     /gene="aac(6')"
                     /locus_tag="ACHGNW_002487"
     CDS             complement(167..715)
                     /gene="aac(6')"
                     /locus_tag="ACHGNW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6926749.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1268..2371
                     /locus_tag="ACHGNW_002488"
     CDS             1268..2371
                     /locus_tag="ACHGNW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6926750.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2368..2574)
                     /locus_tag="ACHGNW_002489"
     CDS             complement(2368..2574)
                     /locus_tag="ACHGNW_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926751.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2564..3631)
                     /gene="rlmN"
                     /locus_tag="ACHGNW_002490"
     CDS             complement(2564..3631)
                     /gene="rlmN"
                     /locus_tag="ACHGNW_002490"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN6926752.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3861..6308)
                     /locus_tag="ACHGNW_002491"
     CDS             complement(3861..6308)
                     /locus_tag="ACHGNW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN6926753.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         1765 a         1362 c         1088 g         2101 t
ORIGIN      
        1 tcttactact cgaaactaag cgactttttc acaacctctg gtacacggtg ttccaaaagt
       61 agcttcttcg gaaataagtt ggaatttcac aaaaattcaa gaagtaattt tcgtaaattc
      121 cttcttattt cttgaagctg attacttctg tcacaacctc tttttattat tgagaatttg
      181 gtcgaggaat aatcgttttt gccatccaaa tatccggttt gtcccagcca tttgcatttg
      241 gtaagacacc tacgatttta taacctaatt tttcatagaa ttcatacgga tgttcacgaa
      301 ggttctggat ggaagccact ttatcaaatg tatgctcata caggtccgtt tgacttaacg
      361 ttgttccatg gtctaaatca tccgtaccta aataaatcgt gattcctcct ctggaagcta
      421 cttctttttc taagtaattg actaatcgag tacctatttg gttctttcgt cgggagcttt
      481 ctacaactaa tggatgcaat tcccaacctg tgataccgta ttgagggatt gcaccaataa
      541 atcctactaa ctcatcttgg tctaccgctg ctaccgcgat tcgttctgga ttcatcattt
      601 cttctacttc ttctgccgag ctgtctccat attcttccgg ccaagtcagt ctcagtaaat
      661 cagaaagctg atctttcaat actggattat tacggtcaaa ttcactgatt atcatacgtc
      721 ttcccccttg ttcttgctta ttctaacagt ccttttgaaa aaacgaaatg ttctcgctca
      781 aaaatcttta gctaccaatc agtatctcta ttctttcatt atttatctta cttataaaat
      841 aaaaaaatta tacagaaaca ctttattcaa aataggaaag atcaggaaag ccatcaattg
      901 gaggaaaaaa ctctgtttgt tcatcatgat atagattatc ttttgcttta aaaaaaacca
      961 attatgaatt gacaaataaa acctgttttc atataataat cttaattttt agaattttta
     1021 gataattaca aaaaagcaaa gggaatcggt agaaatacta agaggaacaa aaattcatta
     1081 ttgtcgtttt caatgttttt gtacttgttc gtttttagga tacttattga tgatttcaag
     1141 gtgttcatgc caccttattc atcacttatc ctgtcttagg ttcacctcta ctccatctac
     1201 agccgcagtc agatcgatca ctctttaaaa ttttaacccg tttcaacgaa gaaagaagga
     1261 aatgaatatg agtcaaagtg ttttcttacc aaattatacc attggaacag atgcgtatga
     1321 aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta gtgctgattg gagggaagac
     1381 tgcactttca aaggcagaaa acaaaataaa agtagctttg acacaggaaa tagaggttgt
     1441 cgccagtctc tggtacgggg gtaattcaac ttatggaaat attgaacagt tgatccagca
     1501 tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt ggtggccgtg cttgcgatac
     1561 tgtaaaagtc attggtgaaa aactagctaa accagtcttc acctttccca ctattggttc
     1621 gaactgttcg cccgtcactg cggtatgtgt catctataac gaagaaggcg taatggacgg
     1681 actcttcttt ccttcaaaac cacctgtaca caccttcatt gatacagcaa ttattgccga
     1741 agcacctgat aaatacttat gggcaggtat tggcgatgct ttatccaaag aagtcgaatc
     1801 tactttttct gctcgtggcg atgatctagg ctacacggat agattaggaa tccaaatcgg
     1861 aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa gcattgaaag atagtgccga
     1921 cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat atcattgtta ctaccggatt
     1981 ggtttctgtt ttagttaaca ataactataa ttctgctatg gcacactcat tctattacgg
     2041 tacgactgtt ctagaaaaaa tggagggata ccttcacgga gcatctgtca gttatggtgt
     2101 actgacctta ttgatgatgg atcagcagtt cgatttgttt gataaaatct atgtgtttat
     2161 gaaaaagaac caacttccag tatcgttaac cgacatgaat ctcaatgtca cgaccgattt
     2221 agaagcaatc ttagataaag ccatggcaac aaatgatatt aaacatgtgc cttatgaaat
     2281 cacccgagaa aaatttaaac aggccattct tgacgtagaa acttatcatg ctacacaaga
     2341 aagccagcaa ggatataaga aaatcagtta aataattgct aaaaccccca tcaagcaggc
     2401 tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt tttcttgtca aaaaaagctt
     2461 tatagctttc gctatagaat cttttctctt atttaagttg aacagttcct taattaagaa
     2521 aactgctcct tttatggtaa ttgaaaaaac gagtaggata cgattatttg gcaactgttt
     2581 ttttcatttg cttgcttcgc aattgtccgc aagctgcatc aatatctgta ccatgttctt
     2641 tacgtatcac acagtttacg ccgtttttct tcaatacatc ataaaatctc aatacatcag
     2701 cttttggact gcgagaatat tgatcatgtt cactgactgg attatatgga atcaagttga
     2761 catatgctaa ttttttctta tcttttaata gatctgctaa ttgttgcgca tgctctggtc
     2821 tatcatttac ttgattcaac atgatatatt caaacgtgat tcgacggttt gttttctcta
     2881 aatactcatc cacagcttcc atcaatttct caattgggaa acttcggttg atccgcatga
     2941 tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat agccagattg acttgcaagc
     3001 cgttattagc gaattccttg attttgtgtg ctaatccact agtggaaact gtaatgtgcc
     3061 gagcaccaat tgccaatcct ttggcatcat tgatgatatg caaaaagtcc attacattgt
     3121 cgtaattatc aaatggctca ccgatcccca ttacaacgac atgtgaaacg cgttcgccaa
     3181 gattgcgctc atcaaaataa tgttggacca tcatgatctg tgcaacaatt tctccagcag
     3241 tcagatcacg gttctttttg agcagcccgc ttgcacaaaa cgtacagccg atattacatc
     3301 ctacttgtgt cgtcacacaa actgacaagc catattcttg tctcatcagt acagtctcaa
     3361 tcatgttttt atcagggagt tcaaacaaat attttactgt accatccgaa gcttcttgga
     3421 cgaccatctg ttttagagga ttgatgataa aatgctccga taatttttcg atcaattgtt
     3481 tcgatagatt cgtcatttct gtgaaattac ttacacgttt ttgatacaac cattcccaaa
     3541 cttgggcagc tcggaatttt ttctcgccgt tttctataaa ccaattgatt aattcttcat
     3601 tcgtcaagcc ataaatggac tgtttttcca aagtattttc cctctttttc tcaaagtttc
     3661 gctacatact atacatgatt catttcctta atgcaatact tcacaaataa atcgaataga
     3721 actttaatct cacaaaaact aaaaagacaa gacatcatac cgtattagaa aagatggaaa
     3781 aaaaccattt tttcttttgg tatcatcatg ccttgtcttt ttattggtta ttcgtttttc
     3841 aacccttcat cacacaagtt ttacatgtca ttatcgtccg gcaacgtttc tgatgtttct
     3901 tcctgctcaa tgaatacttt ccgaggcttg cttccttctg acgggccaac aactccatga
     3961 gcttccagtt catctactaa acgtgctgct cggttgtaac cgatccgaaa tcttctttgg
     4021 agaagggaaa tacttgccgt ttgcatttca acgacaagtg cttttgcttc ttcaaacagc
     4081 tcatcttgtg gctgttctgg agcacctgtc gtttcaactt catctgtcgg catcattttc
     4141 tcttcataat gagcttcttg ctgatctgta acaaattgaa cgacccgttc tacttcgtgg
     4201 tcagaaatga atgctccttg cacacggatc ggtttattct cacccattgg caagaatagc
     4261 atatctcctc gccctaagag cttttctgcc ccattagaat caatgatcgt ccgagaatct
     4321 gtaccgcttg ataccgcaaa tgccattctg gaaggtacat ttgctttgat gattcctgtg
     4381 ataacatcca cacttggacg ttgagtagct aggatcatat gaatccccgc tgcccgagcc
     4441 atttgcgcta aacgaataat cgcatcttca acttcattgc tggctaccat catcaaatca
     4501 gctaattcat ctacgatcac tacgatgaat ggtaaggttg gatgtttcgt tccgttttcc
     4561 aagtttttct gttgaacaaa ttcattgtaa ccagaaatat ttcttactcc ggttgccgcg
     4621 aatttttcat atcgttcttc catttcttgt actacttttt gtaaggcttg cgctgctttt
     4681 ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc cgttgtacat attcaattca
     4741 accattttcg gatcgatcat catcagtttt acttcatggg gcttagcacg cattaatata
     4801 cttgtgataa tcccattaat tgctactgac ttcccactac cagtcgaacc agccacgagt
     4861 aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc cagaaacatc acgtcctaaa
     4921 ggtacttcta gtaatttatc tgggtggttt ggttgtgctt cgatgatttc tctaaaggaa
     4981 accatactga tctttccatt tggtacttca ataccaatca gtgatttacc tggaatcggt
     5041 gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga tatcatctgt taagttcaca
     5101 attttactta cttttacccc tacagccggc tggacttcga attttgtgac ggatggtcct
     5161 aagctggctt tgacgacctt ggcatcgaca ccgaaacttt ggaaagtttg ttctaatacg
     5221 ccgatatttt tttcaatttt tttatactca tcactttgat ccacagttgg tatagaatca
     5281 agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct gttctgcttc aatctcaaat
     5341 tctaatgagg taccgtcatc ctctggcaac tcatctgaaa aaccttctgc tgtattggtc
     5401 tgttttggtg tatctggtac tggtttctct agattcgcag gatggatatt ttcttgaaaa
     5461 ctgtcgattg gaacaaaact tagctgctcc ggctctgtat tttctggttc ctgccattca
     5521 tcagcagtat tttccctttg ctggctcacc gcttttttct tctcatattc tgcagcttct
     5581 ttttctgcag cttcttttgc agctaagcgc cttgcttctt tcgcttctgc gcgctgtttc
     5641 attctctctt cctttgccgc ttgctttcga gcttgcttgg caggatcacc ttccaataat
     5701 tcctgtactc gttctccaat tgattggata ccatttaaca gctgatagcc ttccagatta
     5761 ctgaacagga atgctcctcc tgccagtaat agcactgctg ctaaataact tccaggctgt
     5821 gcaatcaaga agtaagtaaa atgataaagg attgctccaa tcatcccacc accaacgtta
     5881 ttgtctactt gactttgttt tatatcactg gctaacaaat cccatgtcgt ccccatgata
     5941 tttggttccc cagtatggag tttgccaaat aaatgggcat gtaacaataa taagataccg
     6001 aaataaaaga gtatgccgcc aaaccagcgt cggtttttac caattgaaaa ctctgtattc
     6061 ttaatgacaa tccaaaatcc taatacggct aataaaagac agaggatttg gtaagtattt
     6121 cctgcaatga tccgaaagcc gtttgcaatc aatattccta aaaaacctaa ttttaaaaag
     6181 ccgaagacag caaataaaat caaggcaaac ccaacaccaa tcgtcagcat ttgttcctgc
     6241 tgctgttttt gctttttcgt ttttttcgtc tttttccttt ttttagtagg ccgtttcttt
     6301 tgtgccattt attatc
//
LOCUS       DAZBTH010000127         6290 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000127 DAZBTH010000000
VERSION     DAZBTH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.130"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..658
                     /locus_tag="ACHGNW_002492"
     CDS             8..658
                     /locus_tag="ACHGNW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926754.1"
                     /translation="MLGIIWLSYKAYQTRYSLKGSKIRFKRFLLVGVIFALSIVLSGL
                     FGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACYEDMNNIFVDQE
                     KRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEACCNLFSDTIP
                     FSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEWLDQQQQN"
     gene            complement(779..865)
                     /locus_tag="ACHGNW_002493"
     CDS             complement(779..865)
                     /locus_tag="ACHGNW_002493"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6926755.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            1541..2491
                     /locus_tag="ACHGNW_002494"
     CDS             1541..2491
                     /locus_tag="ACHGNW_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN6926756.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            2512..3045
                     /locus_tag="ACHGNW_002495"
     CDS             2512..3045
                     /locus_tag="ACHGNW_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6926757.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            3061..3231
                     /locus_tag="ACHGNW_002496"
     CDS             3061..3231
                     /locus_tag="ACHGNW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926758.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            3681..4214
                     /locus_tag="ACHGNW_002497"
     CDS             3681..4214
                     /locus_tag="ACHGNW_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926759.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            4226..4429
                     /locus_tag="ACHGNW_002498"
     CDS             4226..4429
                     /locus_tag="ACHGNW_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926760.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            4760..5140
                     /locus_tag="ACHGNW_002499"
     CDS             4760..5140
                     /locus_tag="ACHGNW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN6926761.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            5122..6036
                     /locus_tag="ACHGNW_002500"
     CDS             5122..6036
                     /locus_tag="ACHGNW_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN6926762.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(6091..>6290)
                     /locus_tag="ACHGNW_002501"
     CDS             complement(6091..>6290)
                     /locus_tag="ACHGNW_002501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926763.1"
                     /translation="NAISIYSPHTSQKANSVMIKQHKGARISIDRCIITFFCSLFRWF
                     NSAWWNFLTCESIVVCILSLV"
BASE COUNT         2318 a         1096 c         1176 g         1700 t
ORIGIN      
        1 tgccagtatt ttaggaatta tttggcttag ctataaggct tatcaaacaa gatattcatt
       61 aaaaggctct aaaattcgat ttaagcgatt tttattggtc ggggttatat ttgccttatc
      121 aatcgtttta agtggtttat tcggcgtttt agagggtaat aaacgtgttt caagaagcat
      181 tttattaggg gatatcactc aaaagtatga agcagcatgt gataaaaaga aaaaagagca
      241 agcattagca caaaaaatgg aggaatttac cgcttgttat gaggacatga acaatatttt
      301 tgtggatcaa gagaaacgtt taacagataa aaacatgaaa aaactcactc gtttttatca
      361 aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa tatgagcaag taaaaaaaga
      421 cgtgcaatat tttaaagata cgcagacaga agaagcttgt tgtaacttat ttagcgatac
      481 aatccctttt tcaaccagtg aacaagaacg aaaagaacgg caacaatcgg tcacatacga
      541 acgttatgaa acactgcttc agcaagcaac aaacatacaa aatccaacga aaaaagaaac
      601 ggcattaaat tatctagaaa gtgtcaagga atggttggat cagcaacaac aaaattgaat
      661 ttgattagca atcatgttaa actgaacctg caaatgatca aacattagat acacgaaagc
      721 cccagagagg tcggattctc tggggctttt tttcaggttg atgtcgcctt tattgatttt
      781 acttcttgtt tcggttattc aaccaataac tgaataaaga gacacaaact tctacaagaa
      841 gggggcaaat gaaccgttca aacattagat acacctcctt ccagtcgcaa gactgtcgga
      901 taggcgacaa gttattatac cttacaaaat aaaatctgtg tggtgttttt aagtatccct
      961 tattttcggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaag aacaagataa
     1021 ggttttttac ccaagactgt cttaaaacga cttagacgag ctatcagagg cttcccccac
     1081 ctccgttact gacttgtttt tagcaaagga gcctttaggc gactgcgcag tacaatgtga
     1141 gagcatagcg atcacacttt tttagcgacc gtagggagca aggttttgaa ttggcagttt
     1201 tgaaaaatgc gcgaacctgt tggtagaaaa aagaagtggt ttttaggcgg gaaattttgt
     1261 caccctcttt tataatctct ttttaaacct ttttttaaac ctctttaccg tcggctgtgt
     1321 ccttactcct tcaatggata cagcgtttta taaactacat tttgtctgct tttaaactac
     1381 attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg tctgctttta
     1441 aactcttttt ttgcactcac gtatgttata atatgcatta tcacagacaa aacgttgttt
     1501 ataacgtctg tatccattga aggagtaagg aggaatatct ttggcaaatg aaatcgtgaa
     1561 ataccatcat gaattaaata ccattccgtt gcggaaattt acaccagttg aaatgaatct
     1621 atttttctct attgtgtcta gaatgcgcga aaaaggcgat aaaacagtcc gtcttacttt
     1681 tgaccaacta aaagatttaa gtaattataa agcaactgct aataatcgtt tcattaatga
     1741 tttagaaatg acttatgata aattaatgga tttacgtttt ggtaaaagaa gtgctaatgg
     1801 gctcgatata gatagattcg ttatgtttag tgagtttaaa atacgtggaa ctgctaaaga
     1861 tccatatgta gacatacaag tttacgaaaa agctttacct ttactaaaca atttagatga
     1921 gtgggttcgc tattcactcc aacaatttaa cgaattacaa agtagctatg caaagactat
     1981 gtttcgatta ctaaaacaat atcgaactaa aggttttgct tatttttcta aagaggattt
     2041 tcatgaacta ttagatattc caaaaagtta ctggaacaac caagcaaacg ttgataatcg
     2101 tgttataaag cctatacgac aggaattaac cgcaatcttc aaaggattat caatcaaaaa
     2161 gaaatacgga aaagggcgag gaaaacctgt cgttggctat caatttacgt ttaaaccaga
     2221 aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc aaagaagaac aaaataaagc
     2281 attggcttta gctgataaac aaaaagaata cgaagaacct gctacaacaa ttaacgaaaa
     2341 aatcaaacag aagaaacgag aacttgaact cctccaacaa atcaaacaac tagaaaaaga
     2401 gaaagaagct cttgaaaaac aaacagagtc cgcactttcg aaaaaagaga aaaaaagttt
     2461 attatctgat ttagataatt tattccagta gcaacctagg aggcaaccag tatgcaacca
     2521 caagaaacct ttacaataaa ggaattgtca gaacttttta agatgagtag gcaagcaata
     2581 tcgaaacata tccaaaaatt agattcttcc atgatagcta aaaatgagcg tggttataag
     2641 gttgtattgc gttctggagt attgcaactt gctcgcaact taggcaacca aatgttgcta
     2701 gataggttgc acgagtacga acaagacaac gataaaaatg ttaatatacc aagcaaaaac
     2761 gtggttacaa acaagttgct agatcagttg cgtgaagaaa atgaattttt acgagaacaa
     2821 cttgccctca gagagcaaaa taattctcat atacaaaaac tactcgacca gcaacaacaa
     2881 ctcaccctac aagccaacaa acaaatcgaa aaactgcaag aaaaactaca gttgacttat
     2941 acagaagaag ctccagagaa tgactcaacg actctttctg aagcaacaga aacaatagac
     3001 aaacaaattg atactgaaaa aaaatggtgg caactttgga aataactgga gggaacattc
     3061 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg attcagtccg
     3121 atgttattgt cagtgatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     3181 tattacaaca gtcctaacga agaattgttt gtaaaagaaa cagataaata ataaaaaaga
     3241 ctgctctttc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcaacgcg
     3301 cttatgaagc gctaccaaaa atttatcata ttttattaaa aataacaacc cagtaaaaaa
     3361 tgactggtaa aaattcacaa agaaacggaa agaagcaacc gcagaaaatc aagcttaaaa
     3421 agagcttttg acctagacca atcgaaaatc acaaagtgag cgcctgtatc tattgctaca
     3481 ggcgctactt ggcatagtac acattatgtc aacaagaatt taatataaca tattttccta
     3541 atgcattcta actataccac attatcaaaa atataaatta tctttactaa ataaaatatt
     3601 gacaagccta taccttttgt tttatgattt tcacaaagaa aacgttttct atatattttt
     3661 ctgtatcagg aggaaattct atgaaaaaat ttactttagg ggtagctttg ttaactattg
     3721 gggcagttgt tttaccgtca actactatag tttatgcttc agaaaattcc caagtggtat
     3781 cattaaacga aaacaatgct actgaaaatg tagttattga gtacactgaa gatggtatgg
     3841 tcgtaactaa atatgaggat ggttatagtg cggaagattt tgcaaattct tcagatatca
     3901 tgacaagagc tgcagttgca agatggggca aaaaggaata tacaaatatt gctgtaacca
     3961 ctggagtagc tgctaatgct attaatgctg ccttttgggc aggagggaaa gctagcactt
     4021 ctatgtttgg tatcccaggt tgggcaattg gtgctttgtt aacaggtgca agttggacta
     4081 aaaaaggtgc ttctccaggg aatgcagttg caaaagaatg ggacaaaaat ggtaatggct
     4141 ggattggatt ttattaccaa aggggatatg atgcagctgg aagagttgta gctactagat
     4201 attcaacgtt ataagttaag gagtgatgtt tttgatatct aaatcattaa aatcattaaa
     4261 aacatttact aaaaagaatc cgcaaatagc tactattcgt atttttatat ttgctatagc
     4321 tgttgctata attgttaata tgctaagcgg agggcaatta tacaaatctg gtttattttt
     4381 agtgttaatc ttagttgtct tagacataat aaagtctaaa aaaaaataaa tcgatacatt
     4441 ttagaagaat agggttgttt gaatattaca aagcaacaat ttcaccttcg gtgaaataag
     4501 cgaaacttta caaaaagtgg ctagcaaatg ctagcctttt tttgtattgt ggagcgtgga
     4561 ttttgagggg gattcaaggg ggagcttgcg ctcctttgac aagcggttat ttgcaccaaa
     4621 tacgtaggta tttggctagc aaatatagag cttgccaagc cctttagtga gctttagtga
     4681 gctttactta ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg
     4741 tactgaaagg gtgggactta tgagcgaaca aaatcaatat ttggctagca atcattctaa
     4801 aaaatatacc tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt
     4861 tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc
     4921 caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg ctcaggcaat
     4981 cgcgcgcgat ttagccaaag ctggcgggaa tatcaatcaa attgccaagt ggtgcaacac
     5041 acaccaacac gacgttgatc ctggcgacgc ccaacgctta tccgaaaacc taaaaatcat
     5101 gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa gccgaaccac ttcttgtagc
     5161 cgagcgatca attacgccga accacgagca accgtcaaaa cagggataaa ctgtgacatt
     5221 gattatgcca aaagcgaaat gaaacaaatt cgcatgctgt atggaaaaga cgatcgtgta
     5281 caagcacact tattgatcca gtcttttcga ccaggagaaa ttaccgctga aaaagccaat
     5341 caactagggg cagagtatgc gaaaaaaata gcccctgacc accaaatcgc gatttacaca
     5401 catacagaca aagaccatat tcataaccac attgtcatta actccgtcaa tctggaaact
     5461 gggaacaaat ttcaagccca tggtcaagca tttttaaaca aatgttatga cctaaatgac
     5521 gagatttgtc ttgaacatga cttaacaatc actgaacgag ggaagaaacc cgaaaaacga
     5581 accatgtctg aaatcaaatt aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg
     5641 tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa cgtataacca attttgtgat
     5701 tctcttaaaa cattcggcat tcattgcttg aatcgtggga aaaattttac ttacgaattg
     5761 atccacaaaa agaaaaaagt acgatccaac aaactaggaa aggattacga aaaggagacg
     5821 attttacatg agctggacag acgagaacga gcctacaatc aacaacgaac caacgagcta
     5881 cgaagagctc gagctagcat tcgcgaaact gcaaaagaag tacgaagaga acaacgaact
     5941 cttacgactg aacaaccgga aattagaccg actcgaagaa accatgacca acacgttgaa
     6001 agaggagcag actaccaaca agaattatcg ttctaaaacg ttacaagaca tagaaaatga
     6061 aataaagaaa caacgtgctt ttcttgcacc ttataccaaa gaaagaatac aaactacaat
     6121 tgattcacaa gtcaaaaaat tccaccatgc gctattaaac cagcgaaata aagaacagaa
     6181 aaaagtaatt atgcagcgat caatggatat tcttgccccc ttatgttgct taatcatgac
     6241 actgttagcc ttttggctgg tatggggaga ataaatagaa atggcattct
//
LOCUS       DAZBTH010000128         6287 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000128 DAZBTH010000000
VERSION     DAZBTH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.131"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHGNW_002502"
     CDS             190..690
                     /locus_tag="ACHGNW_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN6926764.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHGNW_002503"
     CDS             787..2634
                     /locus_tag="ACHGNW_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN6926765.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACHGNW_002504"
     CDS             2637..3533
                     /locus_tag="ACHGNW_002504"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926766.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACHGNW_002505"
     CDS             3583..3972
                     /locus_tag="ACHGNW_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6926767.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACHGNW_002506"
     CDS             3959..>6287
                     /locus_tag="ACHGNW_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6926768.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZBTH010000129         6218 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000129 DAZBTH010000000
VERSION     DAZBTH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.132"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHGNW_002507"
     CDS             591..1280
                     /locus_tag="ACHGNW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6926769.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHGNW_002508"
     CDS             1267..2475
                     /locus_tag="ACHGNW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6926770.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHGNW_002509"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHGNW_002509"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN6926771.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHGNW_002510"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHGNW_002510"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN6926772.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1890 a         1170 c         1197 g         1961 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgt
//
LOCUS       DAZBTH010000130         6153 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000130 DAZBTH010000000
VERSION     DAZBTH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.133"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1397)
                     /locus_tag="ACHGNW_002511"
     CDS             complement(<1..1397)
                     /locus_tag="ACHGNW_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926773.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNP"
     gene            complement(1695..2630)
                     /gene="murB"
                     /locus_tag="ACHGNW_002512"
     CDS             complement(1695..2630)
                     /gene="murB"
                     /locus_tag="ACHGNW_002512"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN6926774.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2703..3623)
                     /locus_tag="ACHGNW_002513"
     CDS             complement(2703..3623)
                     /locus_tag="ACHGNW_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6926775.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3736..4488
                     /locus_tag="ACHGNW_002514"
     CDS             3736..4488
                     /locus_tag="ACHGNW_002514"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN6926776.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5352..5885
                     /locus_tag="ACHGNW_002515"
     CDS             5352..5885
                     /locus_tag="ACHGNW_002515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN6926777.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1854 a         1178 c         1027 g         2094 t
ORIGIN      
        1 ggatttttcc aaattatctg ttctttatta aatttttcaa gtaaacaatc agataaataa
       61 atattcgtac gagaagtgat tcgactctca atcacaatat tgtaattttt aaatcctttc
      121 acttgagaaa taatgtaatc ggtataagca gatccttgcg aaaaatcaat acagacatga
      181 atcggtgctt caaaatatga taccggacaa ataccgacca ataaaaagag ataatcataa
      241 aataagcgaa tcatgaaacg ttcatctttt cccaagaaat agcgtttatt ctcttgtatg
      301 accttttgga ttgctgtact gaatattgga tacgtctcga agaaaaactg tagctgcttt
      361 ttagacgtaa agctagtcat ctcaaagtcg aatatccaat tttttcgatt gacatgcatc
      421 atacccatac gaagttcttt tcgtatatat agtctttctt cttcagagat ttcaatcgaa
      481 aatggtaatt gatcaatcaa ttccactgac attttttgac taatcatgtg gatatcatga
      541 aaatgagctt ccgcaagtcc aaatgcgcca tctaattcaa attcttcttc cgtgatcaaa
      601 aacaacatga tataagaaat ctcatcaatg atctgctctt tagggatatg aagaaaagaa
      661 atatgtgata aatccttgat gatcttcatt ccttctgagc tcttaaagta ttcatctgaa
      721 aagaaacgtt ttccgattgg ttgattccca taggccctca acaaactaat agtggttagc
      781 aaattgaatt tgattcgttt tgttgcagac agttttaaat tgaacagata ttcaaaatat
      841 tgcacgatac tctttgcttc attcatgacg ctttctctaa atggcataga aaaaccatta
      901 aagaccccga aatatacccc aaataatacg ttccgtatgt ttatttcttc cccaactaat
      961 atgtttttct ttaactttat ttgatgctgt tcaagaaaag agactaattc tcttcttttt
     1021 acataggctc ttgatatgct taaaaaatga ttctctgcaa atttttcgat cgttactttt
     1081 gaatccacga tctccgttaa cagtcgagca atttttgatc gttgaaagta gattcttctt
     1141 aaatttttga tatccgtcaa attcagacgt tcgacatgga tctctgcttc ttctgaaatt
     1201 tggatctctg gctttgtttt gaattcagta aattctttat ttaattctat gatataattt
     1261 cttgttttaa atttagacaa acctaatgtt tcatataact taggcagcac tacataattc
     1321 tttttctcaa attctaaata tctagctagc agcagttttt ctctatcttc ctcttctata
     1381 aggtaatcca acttcatatc ctacacctca aaaaatgcct cataccaatt acttgatatg
     1441 cagatgaaag ttcatctttt cacctatact gattttatga tggattatgc ttgttttttc
     1501 atcagttatt ctcatttttt atcttttttt tgagaatatt gcaaaaaatg gaaaaattta
     1561 tgaggaaata ttctatattt tttgttttat tgtgttaaaa tgtacaagat ttgcgccatt
     1621 tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc ctgctctttt acttatttta
     1681 agttccatta cttattaatt ggctaaactt ctgcttgttt ctgatattgc ttgtttttct
     1741 ccaatgatcc gcacttctgt ttctaaagac acatcaaatt tttctttgat tacttcttgg
     1801 atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat gatcgatatt gacgatgaat
     1861 ccagcatgtt tttcagagac ttgtgcaccg ccccatttca ggccttgcaa tcctgcttgt
     1921 tggatcagct gacctgtata gtgtcctact gggcgcttaa agacacttcc gcatgaaggg
     1981 tattctaatg gctgttttga ttgacgcaat tcggtcaact catccattct tgctttgatt
     2041 gcttcataat cccctgtttg caaactgaat cgagcttcca atacgatagc acgtaattct
     2101 tggactttac tgtgacgata agaaaatacc atttcgtctt ttgtcaaagt tttcaatgtt
     2161 ccatcttcaa gcaaaagatc cacttcagca aaaacatctt tgatctctcc gccatacgca
     2221 cctgcattca tataaaccgc tccaccgaca cttcccggga tgccgcaagc aaattcaaat
     2281 ccagtcaatg attctgccaa agctgcataa gtcgtatcaa tcagtgaggc acctgcttcc
     2341 gcaatgacca tcgttccttt gacatgaatc tgtttcattt cagtcagcat gatgactaca
     2401 ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat ttcctaatac catccaaggt
     2461 atatcatgga ttcggcaata atcgatgatt tgctttactt cttcttgttt tttcgggaat
     2521 gcaaggacat cagctggtcc acctgttttc gtaaacgtat agttcatcaa tggttcatca
     2581 aacaatatag taatcgtggc taagctttca actatttctt ctttattcac tgaggatttt
     2641 cctttctttt ctcaaacgtc ctcattattc tatcacgctt aatacaatct tgccagtctt
     2701 gttcaaagtt gatgacggta gtgatcttta agcacttcct cacataattc atgacttctt
     2761 acaacttctt tttgtttaag caattctgtt tccgatttac ctaaacggat ctgagaaaag
     2821 aaggcctcta ccattggctc aaacccgcgt ttgactagcg tgtttgtcca gtcgccaaat
     2881 tctttcactt tttttccatc agcctcttga atgacaagct gggtcagatc gtttacttca
     2941 taagtaccat ttttacttgt tacttgatat ttttctatat tagcgccact tgctaaatcc
     3001 atcgttaaaa tagccgtttg attttgtgtt tctaattgta aaatggcata ttccaaaaat
     3061 tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg gttcatctaa taaatacaca
     3121 gctgtatcaa taagatgtaa aaacaaatca taaaggacga actctgtcgt ttcttttgcc
     3181 gctattcgat ttttttgcag gtggatcaac cgcttatctg ggatacgttt caattcttct
     3241 accataggag caaagcgccg gttgaaaccg atcatcaaaa ttatttggtt cttatcagct
     3301 aattcttgca attcacgaac ttcatttaga tggacactca gtggtttgtc aatataaaca
     3361 tggataccat tttctagaca ttttttggct aattcataat gtacttttgt tgctgcgtgg
     3421 atcatacatg cttcgatttt ttcttctaaa agctcatcga tcgtttcata tagatgttgg
     3481 aacccgtact ctttttgaat tctttctttt gtttctttat ttcttgttgc aaaataaaaa
     3541 tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg ctttttctgc gatattccct
     3601 acaccgatta caccaatctt catctacttt cctcctggat catttagtta ctttcatctt
     3661 acaccatttt ttactaaaat gaggtacaat agtatcgcaa aggtaaggaa attaattcga
     3721 aggagtgtgc ttttattgaa atttatctct tggaacgtaa acgggcttcg tgccatcgta
     3781 aataaaaatt ttttagaagt ttttcatgag ttagacgccg attttttctg tctgcaggaa
     3841 acgaaactcc aagcaggaca aattgattta gatcttccag gttattatca atattggaat
     3901 tacgcggaac gaaaaggcta ttccggcaca gcgatttttg ctaaaaaacc agcattgaat
     3961 gccacttacg gaatgggtat cgatatccat gatacagaag gccgtctgat tactttggaa
     4021 tattccgatt tctttcttgt aacctgttac acgccaaatt ctcaaagcga attaaaacgt
     4081 ttagattacc gattagaatg ggaagaagcc ttttataatt atttggaaaa tctaaaaaaa
     4141 caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc atcaaaagat tgatttgaaa
     4201 aactggaaaa cgaatcaaaa aaatgctgga ttcactccag aagaaagagc ggctctttct
     4261 cgactgttgg ataacgggtt tatcgataca ttccgttatt tttatccaac ccaggaaggt
     4321 gtctactctt ggtggaacta ccgattcaat tcaaggaaaa acaatgctgg ttggcggatc
     4381 gattactttt tgacgagcaa agatttagag ccaggactag ctgatgcaaa gatccatacg
     4441 gacattatgg gcagtgatca ttgtccagta gaattagatt tgaaataaag aggttgtgaa
     4501 aaagtcgttt agtttcgagt attaagaaaa attttgtgaa aatggctttc cacattttgc
     4561 ataaatttgg cataataccg agaaactggc ttttgaacac tgtttatcga gagattgtga
     4621 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt
     4681 cgtttggttg ggcttattac cgcagataca agcttttgaa caccgtttat taagaggttg
     4741 tgaaaaagct gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt
     4801 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg
     4861 ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4921 atttttcgtt tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag
     4981 aagttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct
     5041 catattttgc tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg
     5101 agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc
     5161 tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa
     5221 atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta
     5281 tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag
     5341 gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata
     5401 gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata
     5461 cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc
     5521 aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt
     5581 ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg
     5641 gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa
     5701 gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt
     5761 tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa
     5821 tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa
     5881 agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt
     5941 cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc
     6001 gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca
     6061 agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt
     6121 accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAZBTH010000131         6136 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000131 DAZBTH010000000
VERSION     DAZBTH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.134"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1367)
                     /locus_tag="ACHGNW_002516"
     CDS             complement(<1..1367)
                     /locus_tag="ACHGNW_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFN6926778.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVG"
     gene            complement(1336..3489)
                     /locus_tag="ACHGNW_002517"
     CDS             complement(1336..3489)
                     /locus_tag="ACHGNW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFN6926779.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            3747..3983
                     /locus_tag="ACHGNW_002518"
     CDS             3747..3983
                     /locus_tag="ACHGNW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFN6926780.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            4002..4217
                     /locus_tag="ACHGNW_002519"
     CDS             4002..4217
                     /locus_tag="ACHGNW_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFN6926781.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(4920..5672)
                     /locus_tag="ACHGNW_002520"
     CDS             complement(4920..5672)
                     /locus_tag="ACHGNW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6926782.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(5687..>6136)
                     /locus_tag="ACHGNW_002521"
     CDS             complement(5687..>6136)
                     /locus_tag="ACHGNW_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6926783.1"
                     /translation="EYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPV
                     PIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLM
                     KKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         1946 a         1062 c          926 g         2202 t
ORIGIN      
        1 tctcctacaa ttaaagacaa ctctccagtt aatccataac gaaacggaaa cgattgagtt
       61 gtttttaacg ttcctttcac tgcataaaaa acacctttct cagtggcagt agaattttct
      121 gaaatttcat ctaaagttcc gtcgatcgta atcggagccc cacctttttg atccaattta
      181 aaatgaacct tcatcccttc tttaatatag gtactttcat tcgcaggtaa aagtgctgta
      241 aatttcattt ttgatttttc gtggatcgga taaatttctg caagcactgt tcctttggct
      301 agttctgctt gtccctttgt ttcttcattt aaatgaaccg ttccagatac aggagctttt
      361 agtattcctt ttgaaagttg atcgtccaaa cttttgataa ctgactggtt cttctcttgt
      421 ttttcattta gctctgtaac tgtttgtttt acagttgtta ttgcttgttc tagctcttgc
      481 tttccttttt ccgtttgact aattctgtca ttctcagaag tagttggttt aactaattta
      541 gctttttctg actgatactg ttctacttct ttttttagtt gctcaatttg ttcatttatg
      601 cttgctgaaa ttgtcagttt cacttgattt ttttgatcat cagaaacatt atttagttgt
      661 tcttgccaat tctcatagtt tgccatcatt tcttttgaaa aatcttttaa tgattgatta
      721 ttactccaag ctacctgtac ttgttgccat tcttgtaatt tactatctga actctcaatt
      781 tgtttttcta gttgtctttt tgtcttttcg tatacttcta attgcttttg atcatttaat
      841 tcactttgct tcaaagcgta gcggagtgat tctttttctg acaacatgct ctttaattga
      901 ttactgtagc caaaactgtc atccgttgaa aacaaatttt ttcctttact tatactttca
      961 ataaatagct gagccatctt tatttgttga gtaatacgtt ggttttcttg ttctatctga
     1021 gccttttcat tctctattaa agaacaatcc aatctaacca acacatcgtc cttatgaaca
     1081 aattgatttt cttttaggaa gttttccatt attttagctt ctaatggcac ttgtaacttc
     1141 tctatttttt cggggttcaa ttgagccgtt gtccgaataa caacctcttt tttagcaaag
     1201 gttaaaaaca atccaataat acataaaaaa agaattacag ggtataacac ccaacggtag
     1261 aagctatgat gttgtttagt atagatacta gaatgttcga accaattatt attctgcata
     1321 aattgtttcc ctctcctaga tttcccataa tttttgatag ataccatcgg caaaacgaag
     1381 ttcatcgtgc gttccttttt ctaccaaatg cccttcatgg agaactaaaa tttgatcaca
     1441 tgcttttgct attggtaaat gatgcgcaat aaaaataatc gttttttctt ttaaattgag
     1501 gagataattc ataattgcat gttctaacaa ggtatctaat ccactagttg cttcatctaa
     1561 aattaatatg tccggatttt ttaataaagc ccgtgctaag gctaatcgtt gtcgttgccc
     1621 tcctgaaaga ttatttccac cttcttccaa caatgtctcg taacgtaatg gctgttgatt
     1681 aataaattca tttagttgga ctatctcaca aacttctatt atttgttcga aacttggtgg
     1741 gctatctaaa ccgaatataa gattctcaat gattgttcca ctaaagaaaa atgactcttg
     1801 aggcaaatat gtcactcgat cgcgtaatat ctgaaaagga atgtctaagt gattaatgtt
     1861 tccataatgg atcgatcctt ctgatgcttc ataaaaacga acaaataact ttgcaagagt
     1921 tgattttcca gaaccactaa ctccaactat agccgtttta ctgtgcgaag gaattgtaca
     1981 tgaaatttcg ttcaacgttg gggccttcat gctatacgag aatgaaacgt tttttacagt
     2041 gatatcttgt tgaaataatt gttcagaaag catatattca aaagactggt tcgatttttc
     2101 tgaatcaatc gcgaaaattt catttaaacg attatttgca acttgagccg tttgcatttt
     2161 cacttgtaaa ttaataatgt tttgtaaagg atctgtcaaa aatactagta aagcattgta
     2221 tgtgataagt tgacctagac taattctatt atccataaca aaataagaac ctagccataa
     2281 aattaatgca ctgctaatta attgaatcaa atgtttaatt ccttgttgaa tattatcaag
     2341 cgtaaccgta cgaaatgatt ttttcattaa tttaacaaat tcatggtcta tacggttata
     2401 tactttctct tcaccgctat atgctttaat tgtttctatt cctttaagac tttctatgat
     2461 actagaattt acagctgcac cggctgccat ttcttccctg ttcgattttt catagctttt
     2521 cacgaaagcg aatattgcaa ctacataaaa aggcaaagag catagagtga ttaaaaacaa
     2581 cgtactattc tgaatagcta acgtaacccc aacaagcaac accataccaa tatctaaaaa
     2641 tattgataac gtagcacttg ctaaagcatc aataatctta ctggcatcaa gaaaacgaga
     2701 aataatttcc cctgattttc tcgtagcaaa aaaattcatt ggtaaagtta acacatgttt
     2761 gaaatactga agcataattg ccatactcat tcgctgtcct aaaataataa gaagataact
     2821 acgactatat tcaaacaata cccgaaataa atagacagcg atcaagccaa tggaaattat
     2881 atttaaagtt gaacgtgctt gattagggat gaaaaaatca agaatacctt gaaaataata
     2941 tgagcttcct atgccaaaaa acgtaatgaa aatcgatgct aaaacgatgt ggaatactaa
     3001 tcctttttgt ttccaaatta taggcaaaaa agaacttaat ccagctactt tttctttaat
     3061 aggaatataa gtttctctag gcataggtaa taaaagaata cctgtccatt ctttttgaaa
     3121 cgcttcaatc gtttttctat tttttccatt agcgggatct gcaatccata gtacgtttcc
     3181 cttcgtttta taaattacaa cgtagtgcat atagctgtta tcaaccaata tatgtgcaat
     3241 taatggtaag ataagatctc tttctttcca gatctcacta tctgcttgaa tagccataca
     3301 atcaaaagac aatttttcaa atgtcttttt caatccaaat gctgaagtac cctctaaatc
     3361 agtcccagat aggtcccgta atttatgaat cgggatatca gttttaaaat attttaaaat
     3421 catcgaaagg caagcaacac cacaatcttt ttcatcttgt tgctgcacaa aaggaaatga
     3481 ttttatcact tatctaacac aactccttct aaaaaatttt tatagaaaaa ttctcaatat
     3541 agctttaata cagtgaaaat attgtctcat aattccacaa aaactttgaa aacagttctt
     3601 gaacactaac tttttcattc ctgaatcaaa tttattttgg aaattttact attcaagaat
     3661 aaacttgata agtttcagga agatttttac tgttaatcaa atacgtatgt tacagtagca
     3721 ccgtaaaaaa tattatttaa agaagcgtgt ttaaacatgc aaaatgtaaa ggaactaagt
     3781 attgtggaaa tgcacaaact gttggtggag gcaataatgc ttggggaaaa ttggggcaag
     3841 ttgtaggtgg acttaccaca ggggcagtag gtggtgcagg tttaggaaca gcaatttgcg
     3901 gaccagcttg cggtgttgtt ggagggttat atggagctgt cgctggaggt gctgcagctg
     3961 gctgggatgc tcgaaaaaaa taaaaaagga gaataagtag tatgcatatt aaaaacacaa
     4021 aaacaacatt tatattatct tcagaagagt tgaaaaatat tcaaggtggg agtgccgtgg
     4081 gagttttagg cactactttt tctggggcaa cagcaggagt aaaactttgt tcagccggag
     4141 gaccttatgc aatagctgct tgtggtgtag gtggagcttt attaggtgct ggattcagca
     4201 tgtggaccgg tgcgtaaaca attaaaaaat atcaaacctc aagattggtt tgtgtcgatt
     4261 acgaacatag gtatgttctt tatcttttca aaagtttgga atggacagaa ctttttacta
     4321 aagcttcttg tgctagtttt tataattata ttaaattttt atcttttatt aaagtcagat
     4381 aaaaattctt tggaaatgta atggtagaga tatttttaca aaaggacttg cagcgctgta
     4441 gttggtgctg caggaggacc tatttcggcc gttacaggag gtattggtaa tgtacttgtc
     4501 ggatgtgcat taaagcctgt tcctgcaagt taaatttata aaaacaggaa agtaattatc
     4561 tcaaatccta tcaagtacaa tcataggatt gcacttgaca ggacaaattt tttaactaaa
     4621 aaatgaaaaa cactttctaa aaattagtag aatttgccaa atctttcgca tcttccacca
     4681 tacatttaat tttaattccc ctttatggta cttaataaaa acaagaaaag aactattcat
     4741 ttggcccttt tttggttcca gaccatagaa atattcctcc agtaactaat attgctgtta
     4801 tgcacgtaac taataacagc tataaaaatt ggtacaactt taataggatt cagaaaaatt
     4861 tgcactagtg aaggaatcta ttttattaga aaaatgcaat ctccctaaag aaaaaaccac
     4921 taaacagtgc tttctttcaa cttctcctta attttttgtg ctttccctaa agtaaagttt
     4981 ctggccaact ctgaatcaaa atatagtgaa cgttttttaa aatctatttc tttaacgtta
     5041 aagatattta ctaaacacgc tcgactaatt cttgttaaca taggatattt cttttcaagc
     5101 tcactaattc ttccataaaa ttcgtactgc ccatctttag tacacaatga taatctatgt
     5161 ggtaattttg aagcttctac aaaataaatg tcatttatat caaaagtaaa tgtttgagaa
     5221 cctatggaaa aaacaaatgc ttgattcgta acctgttttg ttgcatctat cctttcttgc
     5281 gctaataaca ataactcaac aatctcgttt cgatactcat ctagtgtttg atcttttgtt
     5341 acaaatccta acgtttcaac tcttcttttt attgttacgg gtaacatctc atcatgagta
     5401 gttgtaaaaa tgatttttgc ttgaacatca tattttctga ttacttctgc taattctata
     5461 ccgttaactt catgatttaa atcaatatct aagaaatata ttccttgctt tggtcggaat
     5521 tgtttgaggt attttttaac ttctaacgga ctttgtgttt tcaatactat cttaaatact
     5581 ttatcgtgaa aaagaatgaa attatcaata attcgttcta actgatttaa ttgtataatt
     5641 tgatcttcac ataatattat tggataactc ataaatcctc cttccctcaa aacaacaaaa
     5701 taacttgagt agtaaattga tacggatcgt tttgatagtt cacaaacata ttgggattct
     5761 ttttattgat ttcctctata ctagataaac ctaacccttg atgtcccttt tttgttgtta
     5821 ttcctttttt cattagagta tctatagaga gtagtatctc tctacaacta ttcgatatcg
     5881 aaatttctat ctgtttatca tctcggtaaa tagctagaga cagttctcta tcttgactct
     5941 ctatagatgc ttcaattgca ttatcgagaa taattccaag caccctaata cagtcaaata
     6001 ttggaacagg aacttcaata atagtacttg gacattcgaa tttacaacaa atcttattgt
     6061 tatttgcttg gtatagcttc gctatcaaca aacttttcaa atattcattt tttatatttt
     6121 ttaaataacg atattc
//
LOCUS       DAZBTH010000132         6026 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000132 DAZBTH010000000
VERSION     DAZBTH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.135"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1038
                     /locus_tag="ACHGNW_002522"
     CDS             <1..1038
                     /locus_tag="ACHGNW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN6926784.1"
                     /translation="QFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIG
                     FFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINST
                     ADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGN
                     TFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPI
                     LSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGK
                     DIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRNI
                     ISMIKSREHKR"
     gene            1074..1790
                     /locus_tag="ACHGNW_002523"
     CDS             1074..1790
                     /locus_tag="ACHGNW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN6926785.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1809..3392
                     /locus_tag="ACHGNW_002524"
     CDS             1809..3392
                     /locus_tag="ACHGNW_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFN6926786.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3521..4105
                     /locus_tag="ACHGNW_002525"
                     /pseudo
     CDS             3521..4105
                     /locus_tag="ACHGNW_002525"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            4051..4569
                     /locus_tag="ACHGNW_002526"
     CDS             4051..4569
                     /locus_tag="ACHGNW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926787.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4592..5617
                     /locus_tag="ACHGNW_002527"
     CDS             4592..5617
                     /locus_tag="ACHGNW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN6926788.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            5866..>6026
                     /locus_tag="ACHGNW_002528"
     CDS             5866..>6026
                     /locus_tag="ACHGNW_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6926789.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2184 a          692 c         1170 g         1980 t
ORIGIN      
        1 caatttctaa tacaatttgc taaagggtta gaaaaagtta aagctatggg catagcaggt
       61 attatcagca caattgtaac aataattgcc aatattttct tcttgcttat tctgaaatgg
      121 ggattaattg gttttttcat tgcaaatatt ttatcacaga ttatttcagc tacttatctg
      181 tcagtattag tgagaatatg ggaatatatc gattttgagg ctaaagatcc tcagctaaag
      241 agagaaatgc taaattactc gattccatta attgcaactg ttgttggatg gtggattaat
      301 agtacagctg ataggtatgt ggtttccttt atagtaggtg tctcggcgaa tggcttattg
      361 tctgtggcat ataaaatacc tcaaataatt aatactttgc agggaatatt tatacaggcc
      421 tggcaaattt ctgcaattaa agaatatgga gataatgaga ctgcaaaatt ttatggaaat
      481 acatttgtta tgattaattt gttaatgtgc ataacctgtt caagtcttat catattaact
      541 agaccgtttt cacatattct ttatgcgaaa gacttttatg aagcgtggaa atatgttcca
      601 tttttattaa tagctaatgt tttaaattgt gcatcaggtc tgttaggacc tatcttatca
      661 gcaaaaaaag attctaaatc catgatgtgg tctgcaatca taggggcatt agttaatgtg
      721 attatgagtg tagtccttgt ttataagata ggggttcaag gtgccacaat agccactgtt
      781 atatgttcat atgtaatata tgttgttcgt aaacgagctg tgggaaaaga tatttttata
      841 aagcattatc atattgttgt ggaatcttgg ggattattgt ttgttcaggc aatcatagaa
      901 atatatttta aaaattatgt aatagaaatt ataattatga ttattttaat tattataaat
      961 atcagttata ttaagcagtt gtgttataga ggaagaaaca ttatttcaat gattaagagt
     1021 cgagagcaca aacggtagac gatgttattt ttataaaaag atataattta ttaatggtcg
     1081 ataattatga aaggaaaaat attcttaaaa aaaagagtat aggatgtcgt atgaaaatag
     1141 tcgctatagt accaatgaag ttaaataaca aaagattgcc acaaaaaaac acgaaagctt
     1201 ttaccaatgg aaaaccattg tgttattata tactttcaac acttttaaaa gtaaaacaga
     1261 ttgataaagt gtatgtctat tgtagtaacc cagatattaa agaatttata ccagaaggtg
     1321 taacattttt aaagagatct agttccttag atcaggatac aacgagtatt acacaggtat
     1381 tagccggttt cacaaaagaa gtagaggctg atgtatatgt aatgacacac acaacagcac
     1441 catttattag cgaaaaatct attgaaaaag gactagatgc cgtgctgaca ggagaatatg
     1501 attctgcatt tgctgcaaaa aaattgcaag atttcttatg gaaagacggt gaaccattta
     1561 attatgaatt ggacaatatt cctagaacac aggatttgcc gcctttatac gaagaaacta
     1621 gtggatttta tatttataat cgcaatgtga tgaccgagtt gcatagaaga attgggaaaa
     1681 aaccttgcat agttgaagta ggtgagattg agtcagttga tattgatgaa gccgaggatt
     1741 ttatgattgc tgatgcgatt tataatcatc ttattttaca aggtagttaa gacaggggga
     1801 aggttaggat gaacaatgta cagattttag attgcacact tagagatggg ggctattgta
     1861 acaattggga atttggattt gataatacaa aaaaaattat ttctggactt atagaatcag
     1921 gaattgagat tatagaatgt gggtttctca caaataaaac agtatacaat aaaaatgtta
     1981 caaaatatac gaccttagaa gaattagcac agataattcc tgaaaaaaga gaaggaaaat
     2041 tatttgtagc aatgatgaac tatggcgaat ataatataga ggatcttcca gattatgatg
     2101 gttcatctat tgatggaatt aggattgcgt ttcataaaaa aaattgcaat gaagcgttga
     2161 aattatgtcg tgagataaag aaaaaaggct acttagtttt tgttcaagct atggtatctc
     2221 tcaattatac agatgaagag tttttagcag taataaaaaa tgtgaatcag tttaaaccat
     2281 acgcttttta tattgttgat agttttggaa tgatgaaagg aaaagattta actcgtcttt
     2341 tttacatgat tgagcataat ctagaaaaag atatacagat aggttttcac tcacataata
     2401 acatgcagct tgcatattct aatgcacaaa aacttataac cgtacaaaca aatcgaagcc
     2461 tgattattga ttcttcggtg tacggaatgg gaagaggagc aggtaatctt aacacagaac
     2521 tatttgtaga atatttgaat gataatgcag gaaaaaaata tcaattaaaa ccattattat
     2581 atataattga tgaaatacta aatagtttct atcagaagag ttattgggga tattcattac
     2641 caaactatat ttcagcagtt cataatgctc atcctaatta tgcaagctat ctgtcatata
     2701 agaatacttt aacaattgaa gcaatgaatg aaatattcga aatgatggat gaagaaaaaa
     2761 aggtatctta tgataaacaa tatatagaag atttatacta caaatatatg gaaactggga
     2821 aaatacagga ggaatatatg gaagaactga gaaaatttct ctcagacaaa aaaattctac
     2881 tgattgcacc aggaaaaagt tctgtagatg aggtagaaag aataaaagag tttgttacca
     2941 aagaagaagt cgtaataatt agtgtaaatt ttgaatattc tcatatagat atagattacg
     3001 tatttttaag taatttaaga cgttttcatg aattaccgaa agaaagcaga actaaatgta
     3061 ttgttacatc aaatatacaa gcatcggatg tatatttacg gacgagttat aaaaagttgt
     3121 tatctgaaag ggaagttgta agagacaatg caggattaat ggcaattaga tttttagccg
     3181 atatgaatgt ggatgaaata ttcttagctg gatttgatgg atattcacat aatgaagatg
     3241 agaattatgg tgagcaatcc atggaaatta taacaaaatt tgcattatta gatgcaatga
     3301 acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa agtaaaggtg acatttttaa
     3361 cggatccacg ttgggttcga attgataagt agtagtaata atgtttttaa aattttgata
     3421 aagagatagt gagagaataa aaatgggtaa agtcttaaat aaaaaagaag aaaaatattt
     3481 aagggacgat ggggcgagat gaaactctaa ttttgaactt ttaagaatca tttctatatt
     3541 gtgtattgtt gcttaccatg ctaatttgcg tttaaattat aatgcagacg gaattatagt
     3601 acatggttca ttaactgcta accagatttt cttgaattat attggtattt ggggtcagct
     3661 aggagttaat tgttttatat ttatttcggc atatttttta agtgagaaaa gaaacattca
     3721 ctttgaaaat gttttgggaa ttgtattaga atgtattttt tatagtgtat tagtttatgg
     3781 aattctttgt gcatgtggaa tagaaacatt ttcattgaac agctttgtaa aagaaatatt
     3841 atcatttgct ctaggtagat attggtttat tacatcctat ttagcattgt atattctaag
     3901 tccgtattta aatatcatgc ttaacaatct ggaaaaactt cagttgcaaa aaataagtat
     3961 gttattgttt ataggtgtat gctgttatcg tatgttattt aaaacatctt tgtttgggga
     4021 ttgtgctgtt ttaattgcac tatatgtcat atgttataca ctgaaaaaaa cgataataat
     4081 atttttgaga gacacagaat cataggaaca attgtatgta taacagttat accacttctc
     4141 atatccatag gaatactttt caaatttata ccaaatgcaa tggttaacca gatggttcat
     4201 attggttctc cattgatggt tatagcaagt atatttttat tttatgtatt taaaaatttt
     4261 tcattttaca gtaagtatat aaataaactt gcgagaacca gtttaggggt atatatttta
     4321 catgaaaatc attatttcat tgatggtatg aagtctagag gcttaatatg ggatgggata
     4381 tttggattag gcaaaaggtt tttagatagg gatttttgga tttggtttat aattgcagtt
     4441 gtagttgtgt gcataatttg catggctatc gatgttgtaa gagaaaaatt tgaaagacag
     4501 atttttaaaa ttaagagtgt aaagaacctg tgtaaactta tgaatcatgt tataaatata
     4561 tacccataat taataaatga ggagaaaaga agtgaaagtt atatcattcg gagaattatt
     4621 attgagactt gcagcacccg gttattcaaa gttatttcaa agagatagtt tggagactac
     4681 attctgcgga ggggaagcta atgtagcggt atccttagca atatttggat atcaatcaga
     4741 atttataaca aagcttccgg attcagatgt tggatatgcg gcagctaatg ctttgcgtta
     4801 ttttggagta aatgttgata atattatatt tggagaggga cgcatgggac tgtattattt
     4861 agaaaaagga gcagctcaga gaccatctaa agtaatttat gacagaaaat tttctgcaat
     4921 ttcgttagct gatagtgatg aatttaagtg gaaggagatc ctaaacgatg caaattggtt
     4981 tcattggaca gggataaatc cagcattgtc tgagaatctc tttgagacgt ccttagatgc
     5041 atgtcaggct gccaaaaaaa gaggtataac aatttcttgt gatttaaatt atcgatccaa
     5101 gctttggaca tcagaggaag caaaagaaaa gatggaacag atactaccat tggttgatat
     5161 ttgtatttgt aatgaagaag atgcagaaaa agtgctagga attaaagctg agcatacaaa
     5221 tattaatagt ggagaattaa ataaagatgg ctatgtgcag gtggctaggg agattcacaa
     5281 gaagtttgga tgtaggtatg tagctacaac tctgcgtaga agttattcgg caagtagaaa
     5341 tggttggagt gcaatgttat acgaagcaga aaaagataaa gcgtattttt ccacagaata
     5401 caacatacaa attgttgatc gcgttggtgg tggagacagc ttttctgctg gtataatata
     5461 tgcgttatgt gaaaatatgt ccggacaaaa ggctattgaa tttgcgactg catctagttg
     5521 tttaaaacat actatagaag gtgattttaa tcgaactaca attgaggatg tatataattt
     5581 aatgaatagc ggtggaaatg gtcgcgtgtt gcgttagtca acatataaag ataaatcaca
     5641 gtaattttta tggtgattat ctgtcttagt agtgattaga ataacgtgaa aataaattgt
     5701 tgagcgttta tactgttttg aaaaataaat ttaacatatt gaaaaataca aatatatacg
     5761 tatattataa agcgccgatt gtaaaattaa gctagacaac taaaacagtc atttgtgcta
     5821 cactcaaaat agttccgacc aaagaattat aaggagtgag tgcaaataac ctatacctat
     5881 cttacaacgg atgaacttgt aataatagag tcttatttga aaattaacca atctgttgct
     5941 aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc ataaagtata tctgttcttc
     6001 aagcaaagaa aatcagcctt agaata
//
LOCUS       DAZBTH010000133         6002 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000133 DAZBTH010000000
VERSION     DAZBTH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.136"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHGNW_002529"
     CDS             <1..395
                     /locus_tag="ACHGNW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN6926790.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHGNW_002530"
     CDS             398..1228
                     /locus_tag="ACHGNW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFN6926791.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHGNW_002531"
     CDS             1244..2095
                     /locus_tag="ACHGNW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6926792.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHGNW_002532"
     CDS             2092..2451
                     /locus_tag="ACHGNW_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN6926793.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHGNW_002533"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHGNW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHGNW_002534"
     CDS             2624..2929
                     /locus_tag="ACHGNW_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926794.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHGNW_002535"
     CDS             2929..3240
                     /locus_tag="ACHGNW_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN6926795.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHGNW_002536"
     CDS             3237..3410
                     /locus_tag="ACHGNW_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926796.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHGNW_002537"
     CDS             3407..3823
                     /locus_tag="ACHGNW_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN6926797.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHGNW_002538"
     CDS             3823..4491
                     /locus_tag="ACHGNW_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFN6926798.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHGNW_002539"
     CDS             4488..4865
                     /locus_tag="ACHGNW_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926799.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHGNW_002540"
     CDS             4862..5161
                     /locus_tag="ACHGNW_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926800.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHGNW_002541"
     CDS             5151..5309
                     /locus_tag="ACHGNW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926801.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHGNW_002542"
     CDS             5403..5819
                     /locus_tag="ACHGNW_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6926802.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAZBTH010000134         5924 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000134 DAZBTH010000000
VERSION     DAZBTH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.137"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1169)
                     /locus_tag="ACHGNW_002543"
     CDS             complement(75..1169)
                     /locus_tag="ACHGNW_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN6926803.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="ACHGNW_002544"
     CDS             1358..2119
                     /locus_tag="ACHGNW_002544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6926804.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACHGNW_002545"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACHGNW_002545"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6926805.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="ACHGNW_002546"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="ACHGNW_002546"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN6926806.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4811)
                     /locus_tag="ACHGNW_002547"
     CDS             complement(3759..4811)
                     /locus_tag="ACHGNW_002547"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN6926807.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4937..5857
                     /locus_tag="ACHGNW_002548"
     CDS             4937..5857
                     /locus_tag="ACHGNW_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN6926808.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DAZBTH010000135         5824 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000135 DAZBTH010000000
VERSION     DAZBTH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.138"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1142)
                     /locus_tag="ACHGNW_002549"
     CDS             complement(45..1142)
                     /locus_tag="ACHGNW_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6926809.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1250..3277)
                     /locus_tag="ACHGNW_002550"
     CDS             complement(1250..3277)
                     /locus_tag="ACHGNW_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN6926810.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3422..4150)
                     /locus_tag="ACHGNW_002551"
     CDS             complement(3422..4150)
                     /locus_tag="ACHGNW_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6926811.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            4583..5662
                     /locus_tag="ACHGNW_002552"
     CDS             4583..5662
                     /locus_tag="ACHGNW_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6926812.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1626 a         1281 c         1086 g         1831 t
ORIGIN      
        1 aaccatccta caataataaa aatttactat ttgttatctt aaccttactt ttctttctct
       61 tctttttttc tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa
      121 gctactaaga tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat
      181 tccttggcta cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag
      241 ttgatcgtag tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa
      301 acagtcattg taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga
      361 aaagaaaaat ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact
      421 tcagaatgtc ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt
      481 tgatcatttt gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact
      541 tcatgaagag taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata
      601 atataggtgt attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt
      661 tccgtctgga tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc
      721 ggcatcctga catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt
      781 ggatagctca cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt
      841 tttccgtatt caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt
      901 ttcacttctt tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga
      961 tcaagttgaa gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc
     1021 gtcactgaaa aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa
     1081 ataactagga gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc
     1141 atatgtaaga gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac
     1201 ctgtgccctt cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg
     1261 ggacatctgc aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta
     1321 caggaatatc tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc
     1381 tttcaccctt ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat
     1441 tttcagaggt aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt
     1501 tctcttgttt caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc
     1561 cacctgaaaa ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat
     1621 tgacatattg aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat
     1681 cctctgtatt ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc
     1741 cttcaatatc aataggagtt tctttatact tctctcctat tttcccagtg attgttttca
     1801 tttcagcaat atcaatctga ctgtctttat ctttataata aacatggata tctatctcct
     1861 tcagctttgc gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa
     1921 ggtattcaaa atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca
     1981 attcgattat taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat
     2041 ttccggttat caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg
     2101 gaatagttaa ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat
     2161 ccccctcgac cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga
     2221 tggccccata atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa
     2281 gtcgcgtgac agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc
     2341 aatttacttc tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagtgac
     2401 cgacaccttc caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca
     2461 agcgccatgt gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag
     2521 aataattatt attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat
     2581 cagcagttat ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact
     2641 caatcgagag cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc
     2701 ccatacctgg cgatccaata acttgactgg gattactagc cattctagga tcgccagtca
     2761 atgtaaaggt catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt
     2821 taaatgtaaa atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag
     2881 acctttctgc tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac
     2941 cgataggtgt ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa
     3001 ccgcagaaat tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt
     3061 cagtggtctc tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg
     3121 attctattaa actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat
     3181 gctccaactg attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga
     3241 gactgcttaa aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt
     3301 aaattcctag atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg
     3361 ctcgattacc tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa
     3421 actagttgtt aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta
     3481 acttgacaga tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg
     3541 caccaaatcg atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg
     3601 tattgtttcc tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg
     3661 gatcaaccaa agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat
     3721 ccccactggc agttttaaac tgttctggtt gagagacact taatttccac ccagataagg
     3781 tacttctcct atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat
     3841 cagcagctgc ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt
     3901 ttgatccata atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat
     3961 cgacaggttt attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc
     4021 ctgcttcaaa tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag
     4081 aaacaaaagt cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt
     4141 cttttttcat cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt
     4201 aatccatagc aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt
     4261 tggtcctatg tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta
     4321 ttgcaacttg atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg
     4381 taagtccccc tcctatctat caaaatgtga cttgactgcc tagacttcaa tcaatattaa
     4441 ttataattaa ataaaaaatg tatattatat ttttttggcg gattttataa ttaacttagc
     4501 catttggaaa taaaaaaacg ccacgtgaat tccatgttat attgaagtta ccacaactct
     4561 caatagacaa ggatgaattc acatgacgca aaaccatgat aacacagttt cacgcaaggg
     4621 aaaacaccta acctactcag aacgttgcca aatcgccatc ctgaaaaagg aaaactattc
     4681 caaccgtcag gttgcggatg tcctgggctg cgtgccgcag accataaaca acgaaatcaa
     4741 acgcgggacc gttactcagc ttaaccgcca gaatcagaac gggaaggtct acgactatta
     4801 tcatgacgtc tacgacgccg atgccgggca ggcggcctac gagaagaacc gcttgaactg
     4861 cggcagacgg cccttatggg ccgacacgga cgccttcatt gaatgggcgg acgacatgat
     4921 gctgttggaa aaatggtccc cggacgtggt ggtgggcttt gccagaaaac acgaattgtt
     4981 cgacccggcc attatccctg ggaccaccac cctctatcag tggatcgacc gtggcatcat
     5041 gaaaacgaag aatatggacc tgctagaaaa actgtcccgc aaaccgaaat cgtcccgtca
     5101 tggcaaccgc ccgaataaac gggtgctagg gaaatccatt gaagagcgcc cggaggcggt
     5161 tggtacccgg aaaaccttcg gccattggga agtcgacacg gtcgtaggaa acaagaccaa
     5221 gtcggatgcc gtgctgctga cgttggtgga acggcagaca cgcttcgagg tcattctgaa
     5281 gctggccggg aaggatgccg aatcggtcaa ccaagcggtc cgacagctga gagaacgggc
     5341 tggtgcgtac ttcggccagc tgttcaagac ggttacctcg gacaacggct cggagttcgc
     5401 gggactgcac gatgccttgc aggatgtggc aaacatctac ttcagccacc cctatgcttc
     5461 ctgggagcgc gggacgagcg aaaaccaaca caagctgatt cgccgtttca tccccaaggg
     5521 cagcccaatc ggcaacgtga gcgaaaacca aatcttgcgg attcagcgtt ggatgaacga
     5581 ttacccgcga aagattttgg gctatgcaac gccgcatgac acgttcgtcc aggcatttag
     5641 gcaggaacgg ttgacggctt gacacgtggg cccttgacaa cgagcctccc tcggcatggc
     5701 ttgtgtctgg aaagctggcc ggggccagcc agtcgggaga ctaccatgcc tcagctcatt
     5761 gtcaagggcc cactcgatta gaaaaattag acggttggtg gtaacgagca accgggtcac
     5821 catg
//
LOCUS       DAZBTH010000136         5821 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000136 DAZBTH010000000
VERSION     DAZBTH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.139"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..95
                     /locus_tag="ACHGNW_002553"
     tRNA            23..95
                     /locus_tag="ACHGNW_002553"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Lys,seq:ctt)
     gene            257..478
                     /locus_tag="ACHGNW_002554"
     CDS             257..478
                     /locus_tag="ACHGNW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926813.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(802..1047)
                     /locus_tag="ACHGNW_002555"
     CDS             complement(802..1047)
                     /locus_tag="ACHGNW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926814.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1041..1442)
                     /locus_tag="ACHGNW_002556"
     CDS             complement(1041..1442)
                     /locus_tag="ACHGNW_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN6926815.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1614..3422)
                     /gene="uidA"
                     /locus_tag="ACHGNW_002557"
     CDS             complement(1614..3422)
                     /gene="uidA"
                     /locus_tag="ACHGNW_002557"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN6926816.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3450..5216)
                     /locus_tag="ACHGNW_002558"
     CDS             complement(3450..5216)
                     /locus_tag="ACHGNW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN6926817.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(5229..>5821)
                     /locus_tag="ACHGNW_002559"
     CDS             complement(5229..>5821)
                     /locus_tag="ACHGNW_002559"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6926818.1"
                     /translation="EKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQSEDLLKALKW
                     SEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEEWERHELLSLV
                     DELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKE
                     SWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
BASE COUNT         1812 a         1115 c          756 g         2138 t
ORIGIN      
        1 tagaataaca aagtattaat ttgggctgtt agctcagttg gtagagcaga tgactcttaa
       61 tcatcgggtc gcgggttcga gcccctcaca gcccattggg tgccaaaccc ttaaatacag
      121 tgtaaacgtt ggtgcttagc atttagaaaa cgtatatact gtattttttt gttttcaaaa
      181 gagactgtag tacccctttt tttcactaaa agcccactgt agtcattgat atttttgata
      241 agaaaggaac ataattatgc gtaatgaaat tgctaatata acaataatcc gagaaacttt
      301 acaaaaccat actgcaaatg agatctctaa acatacagga ttaaatttga gtactataaa
      361 aaaactaaaa agtggagaac gttcaataga aaaactaaat ttacatgatg ctatttgcct
      421 cactgaattt ggtcttaaaa ataatagaaa aaatgttgaa atcaacatat ggaaataatt
      481 cttaatcagc tgcttttgtc aaaagcacat aggaatttca cctctctcca agtttacggc
      541 gagccttacg gaagtatcat tatcccaact tcaaatcatc gctttttatt gactataaaa
      601 attattttag aatgtttatc gctccttctt tacagtaaga aatcatggcg caactaaaat
      661 atgctccttg aagtgttaac gtactgatag aatgtattta aatttcaatg atcaataggt
      721 tttctgttta aaagaatcta ctaatccttt aactattaga agattctttt aaacagaata
      781 agtggaaagg ggacttttta gttagttaat tttaaatttt aagatcgctt ctatcaattt
      841 aatttctagc tgtcttagca tatactcatc tacataggta ataaattgcc ccgcctcatt
      901 aaatccattt gtcaaacata acttagctat ataactatga taatgtttga gtatcatctt
      961 gattgaaatt ggatcttctt ctagagcaga ttggataatg gatagaggaa ctaatggata
     1021 ctgccgtttt ttggggttgt ctaccataag tcatacccca tttttaattg ttcaactgcc
     1081 ttctttcgtt gataccagat accacttgta ctcatattga ggattttacc gatttcagta
     1141 tcattaaaac ctgcaaaata ataaagtagg ataacctttc ttcttatttc cgataaatga
     1201 tcaatggctg ccgataactt ttgatctgaa attaatattt ccattccaaa taagaaatag
     1261 acatcttgtt catcaaaatg agtttgggag tacttacctt catttgaatt cgtcaatggt
     1321 tcctcattat ctgttatgtt aatgtttcgt tttcgaatag acttagtttc gtttcgtaat
     1381 acttttttac agtaggcatc aaattgatgt tgtatagatt tctcattgta attttgttgc
     1441 atgataaata tgctcctttt ttcccctttc cactaataca acaatcagaa agcgcctttt
     1501 tttcgaaaaa actagaaaaa aatatccttc tttaaacaga aaatcttttt ataaaaaaaa
     1561 ggagttggca attaagcagc cactcctttt ttatattcct tataaaaggt aaattatttt
     1621 ttataactga aatttggtat tgatgaccat cgtttcttta gatagtgagc aatcatttta
     1681 ggctttctat ctctagtaaa gattcctttc ttatttcctt gcactcttaa aatcccttga
     1741 cttgtttcaa aatctgcaaa attccacact tgttcaccaa caaaattgtc acatttatct
     1801 attacttcgt gatttgcttc gtaataaccc acttgatact cttctgtaaa cattgttggt
     1861 actgtatcat gaagtccagc tactgtatct gctccatatt ctgtaaacat tattggcgta
     1921 tcgggacatc tttccatcca tccatctagc tcttttttca acagatattt tgcatgtttt
     1981 aggtctccac ctgaaacata ccaaccataa tagcgattta aacataaaac atcgacaata
     2041 tctcctactt tacaaatatc tggtgttgac tttaagtaag taacgattgt tacaggtctc
     2101 ttctgagggt caagttcttt agtcaattca actaaaggtt caaaatattc tttagcgcct
     2161 tcagaatccg aatctggttc atttgcaata gaccacatta cgacactcgg atggttcttg
     2221 tcgcgttcta tcaattcctt aatcgccatt ttgtgagcat tttctgtccg tatttctttc
     2281 caagtatcat gtttttctac gttgtcatct aataaagtag ccatgaaatt caaatgtaat
     2341 cctactgcag gtacttcatc aataactaca ataccttctc gatctgctaa gcgcataatt
     2401 tcttctgaat aaggataatg tgaagtccta aatgaattag caccgatcca tttcattaaa
     2461 ttaaaatctt tgatatttac tgcttcattc aaaccacgac cattaatata cgtgtcctca
     2521 tgcttcccaa atcccttaaa gtaaaaaggt ttattattaa tcaaaaactt cccattcgaa
     2581 acctgaatac tacgaatgcc aaattcttct gtgtacgtat caatcacttt attttcgtta
     2641 attaaactaa ctttcatact atataagtaa gcatttagag gctcccaaag tgttgggttc
     2701 ttaagttgaa tcactccttc tttcccctta gcttctgcta taagttgagc ttcttcgtca
     2761 tagattgcta ttagaatttc tccggtccat tctgtatcga tattaaattc tacatttgca
     2821 tctgaatcaa ttatttttgt attaatagtg acatccttta tatagtcttt cggagttgta
     2881 tacaatttaa cgggtctatt aatgcctgca taattgaaaa agtcaaaatt aaccaaattt
     2941 tcttttttta tgtctccatc ttcctgtatc gtttctttca aaatccctac aggtaaagtg
     3001 ctatcgtcaa taacattgtt tacagccact gtaattctgt tacttccatc ttttataaac
     3061 tgattaattt ctgattcaaa tggcgtaaac cctcctttgt gttgattaat caattttcca
     3121 ttcaaaaata cttttgcttc atgtgttgca gatccaaaac gaagaactaa tctttcttct
     3181 aataattttt taggaagaat cacttctttt tcataccaca cccatccaac atgatttcta
     3241 atgttctctg tttcaaataa atcattataa gaagaaggaa ctgaaattgg ataactctct
     3301 ttttcaacca ttggttctaa agcaagttct tcttgtaagt catcatttgt atctaactta
     3361 aatctccaaa gaccgcttaa atcaattact tgcctcgtct ctgtcataat gggatataac
     3421 atgtaaatca ctcctatata atttaaatat taaataaatg tatcctttaa cccacaataa
     3481 gcatctattg gacttattcc cttaaattca gaaaggccca ttagtttgtc cactaatatt
     3541 tctaaagttt cttgttttcc atcataagta ttgatataag tttttacttg aggcacatca
     3601 gctaaatgaa acggacactg tacagataca aaaattgttg gaatttcatg aatataaaat
     3661 ggaatatccg gagtaccttt acttgctggc caaacaattc tttgaactgt acctgggtta
     3721 acattagcta tattaataat caagtcatat ttttcagtta aatctttgat tggtctctta
     3781 gcagcatata ttccagctat atttttaatt ttttcttctt gcgataatcc tttcaattct
     3841 tcttcaggtg aaatgtattt ttcaacttca aatccttttg ctgacaaaat ctgtttcaat
     3901 aaatcaatgg cctgaggtct atttccagca actacttcac caaacccacc ttttacccca
     3961 tgtacatcta ccagtaaaat ccttttatat ttttctggta caataggcca aatatcttgt
     4021 ttatctttaa ctagtgtaat tgcttgatca gctacctctt taaaaattga tttattttca
     4081 attaaaccaa ttttttccat agcttcttgt ttcggtaaca aaattttatt cttttctttt
     4141 ttatgcaaac ctagtttagc ctttaagcct aaaattcttc ttaatgcatc acgtaaacgc
     4201 tcttctgaaa taattccttt gtaataacct tccatcatat aattaaagtc ttcatctgga
     4261 tcattaaaaa ataggaatag atcacatcca gccgcaatag aagtgggcaa taattctgac
     4321 cgtttcattg ccccagtcat tcctaacata tgactagcat cagtaactac taatccatta
     4381 aacccgagtt ttcctctaag taagtcagtg attaattctt ttgataaagt tgcaggaagt
     4441 aaatcttcat ctttcagatc aagattaaaa tgttttgaat aagctggcat atggatatga
     4501 cctgccataa ttgaaggtaa tccagcttct attaagcctg agtatacttt tccaaaagaa
     4561 ttatcccatt cttctgtact taaactatta atactaaaag atagatgttg atctcgttcg
     4621 tcaataccgt caccagggaa atgtttagct gcaggcaaaa ttccactctc ttgaatacct
     4681 ttcatataag ctaaactaag ttctaaaact ttatcagggc tttgtccaaa agatcgacta
     4741 gaaatgatag gatttctcca atttctatta atatctacaa taggagcaaa actccagtta
     4801 catccaatag ccgatgcttc aattccagaa actctcccta attcataagc atatttcgca
     4861 tcatttgttg caccaatttt tacttctaca ccaacttcgg taccatctgt acaagctccg
     4921 ttaccacctg cttctgtatt agcagcaatt aataaaggaa ttttgctttc tgtttgtagg
     4981 atatagtttt gatcatatac ctcatcagca gttcctggat tatatcgtac agctccaata
     5041 tggtactgat tgaccatact acttaagtaa tttttatcac gactagatcc catattaaca
     5101 aataattgac ctattttttc ttctaaagtc atagcatcaa tcgtctcttc aatccaagaa
     5161 atagcatctt catctaaatg atatggtttt tttcgtaagt ctacttttat agtcattttc
     5221 tttctcctct acttattagt aagatttttc caaaaagtat ctatatattt ttgttcaata
     5281 tctatatact ctcccgataa acatttaaat aaaatatcag ttcttaagtt ttcccatgat
     5341 tctttttctt tattaaccaa aataggataa aagaaaactt tattcttatt tgcagcttct
     5401 agatcaccag gagaatcacc aatcattaat atcttatcca aatcacaacc attttcaatt
     5461 acacttcgaa taatgtcttc tttttttcct ttatcttgac aacacagttc atcaactaag
     5521 gataataatt catgtctttc ccattcttct tgaatagctt ccttattagc tgagcttaca
     5581 ataattatct tcccatattt tttcagagct tttatagtat ctctagcact taaaaagggt
     5641 ttatctaatc ctttagcttg actaatctgt cgattgactt cttccgacca ttttaaagct
     5701 tttaataaat cctctgactg agagtattct atttccctct tcaaagatgg attagacaat
     5761 tcttttgtta cagataccca gtgctttaat cgattaatcg atggtacatc atttttttct
     5821 a
//
LOCUS       DAZBTH010000137         5809 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000137 DAZBTH010000000
VERSION     DAZBTH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.140"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACHGNW_002560"
     CDS             450..1604
                     /locus_tag="ACHGNW_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFN6926819.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHGNW_002561"
     CDS             1577..2020
                     /locus_tag="ACHGNW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926820.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHGNW_002562"
     CDS             3143..4075
                     /locus_tag="ACHGNW_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6926821.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHGNW_002563"
     CDS             4092..4607
                     /locus_tag="ACHGNW_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6926822.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHGNW_002564"
     CDS             4903..5184
                     /locus_tag="ACHGNW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926823.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHGNW_002565"
     CDS             5186..5602
                     /locus_tag="ACHGNW_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926824.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZBTH010000138         5790 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000138 DAZBTH010000000
VERSION     DAZBTH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.141"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHGNW_002566"
     CDS             complement(99..1229)
                     /locus_tag="ACHGNW_002566"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFN6926825.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACHGNW_002567"
     CDS             complement(1877..3079)
                     /locus_tag="ACHGNW_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6926826.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACHGNW_002568"
     CDS             complement(3139..3342)
                     /locus_tag="ACHGNW_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6926827.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4548)
                     /locus_tag="ACHGNW_002569"
     CDS             complement(3484..4548)
                     /locus_tag="ACHGNW_002569"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6926828.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACHGNW_002570"
     CDS             complement(4609..4716)
                     /locus_tag="ACHGNW_002570"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926829.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACHGNW_002571"
     CDS             4879..5691
                     /locus_tag="ACHGNW_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926830.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAZBTH010000139         5603 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000139 DAZBTH010000000
VERSION     DAZBTH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.144"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..769
                     /locus_tag="ACHGNW_002572"
     CDS             68..769
                     /locus_tag="ACHGNW_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN6926831.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            766..1887
                     /locus_tag="ACHGNW_002573"
     CDS             766..1887
                     /locus_tag="ACHGNW_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN6926832.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1902..3134
                     /gene="pepT"
                     /locus_tag="ACHGNW_002574"
     CDS             1902..3134
                     /gene="pepT"
                     /locus_tag="ACHGNW_002574"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN6926833.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3266..4174
                     /locus_tag="ACHGNW_002575"
     CDS             3266..4174
                     /locus_tag="ACHGNW_002575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6926834.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4209..4481
                     /locus_tag="ACHGNW_002576"
     CDS             4209..4481
                     /locus_tag="ACHGNW_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN6926835.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4492..5538
                     /locus_tag="ACHGNW_002577"
     CDS             4492..5538
                     /locus_tag="ACHGNW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN6926836.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1822 a          972 c         1215 g         1594 t
ORIGIN      
        1 atttttttat tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag
       61 tgaagctatg aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc
      121 tgaatccgct cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat
      181 attaagagga aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc
      241 agcacgtaag caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa
      301 tggtctagat gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg
      361 aggttcactg atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca
      421 agaacgtttg atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga
      481 aaatcagtat aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat
      541 cattgtagcc gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg
      601 gccatttctg cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca
      661 acaaagaaaa gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa
      721 acaaatcgag caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga
      781 cgcactttca tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac
      841 ccagtcggac tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta
      901 gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct
      961 aaacatcctc caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa
     1021 atgtatgcag atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat
     1081 attatttggg atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt
     1141 tatttagtaa agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat
     1201 catgcacaga aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat
     1261 accggtacaa gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa
     1321 ccagcgattg ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt
     1381 ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag
     1441 gaacctgctt atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga
     1501 gtaggtgaat tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg
     1561 ttccaactag atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc
     1621 attgcgattt gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca
     1681 gatgtctata tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg
     1741 ttgattgcaa ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag
     1801 aaatttgaat cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca
     1861 aatacaaacc catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc
     1921 cacgtttttt acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc
     1981 catcgactca gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag
     2041 gcttagctga tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca
     2101 atgttgatca tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta
     2161 atgcagtagg agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac
     2221 tagatgcgga agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg
     2281 aaggccacac attgatcaca acagatggca gtacattact gggagcagat gacaaatcag
     2341 ggattgcgga aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg
     2401 gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg
     2461 atgttgagca attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt
     2521 tgcagtatga aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac
     2581 atccagggac ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata
     2641 atcagcttcc agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt
     2701 tgatggcatt ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg
     2761 atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc
     2821 agcgatttga tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag
     2881 aaatcattga aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg
     2941 gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca
     3001 tgggtattcc gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat
     3061 ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga
     3121 acacaaagag ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg
     3181 aatgtcatct gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt
     3241 ttgaagagta aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc
     3301 agggaaagat gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca
     3361 gccagaatta tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt
     3421 caagaaagca gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat
     3481 tcatcatatc gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc
     3541 aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc
     3601 tgctgacgtg atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga
     3661 agtcattatc tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca
     3721 agaagaaaaa cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct
     3781 gaagaagaaa aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc
     3841 ccaactgttg acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc
     3901 agcgacccta gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg
     3961 atttattttt tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac
     4021 ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac
     4081 ggatggagac acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac
     4141 agtttattca ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa
     4201 gaagagtaat ggatgtattg ttacacaatc ctttgtggtt ttaccttttc attgtgaatt
     4261 tttacttatt ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg
     4321 ttcctgagaa taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct
     4381 ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag
     4441 gtgtacttgt tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct
     4501 aaaggaacga aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt
     4561 atttattatt taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt
     4621 gttgttgttt tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc
     4681 tcaatcaaag aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg
     4741 acctctagct atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg
     4801 gagatttatt tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca
     4861 ctccatatga ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc
     4921 caattctctc ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc
     4981 cttttgacga tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa
     5041 atattttttg tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg
     5101 gatacatgca atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac
     5161 aggactgcgc tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta
     5221 cttccttatt tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg
     5281 tttatcagct ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc
     5341 tttgaatttg tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct
     5401 tcgcttttac tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat
     5461 gtgattgctg aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat
     5521 aaagaaaatc aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa
     5581 cgttatggat aagataattt tac
//
LOCUS       DAZBTH010000140         5583 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000140 DAZBTH010000000
VERSION     DAZBTH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.145"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..252)
                     /locus_tag="ACHGNW_002578"
     CDS             complement(31..252)
                     /locus_tag="ACHGNW_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926837.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(249..539)
                     /locus_tag="ACHGNW_002579"
     CDS             complement(249..539)
                     /locus_tag="ACHGNW_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926838.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(536..1720)
                     /gene="mobT"
                     /locus_tag="ACHGNW_002580"
     CDS             complement(536..1720)
                     /gene="mobT"
                     /locus_tag="ACHGNW_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN6926839.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1902..3305)
                     /locus_tag="ACHGNW_002581"
     CDS             complement(1902..3305)
                     /locus_tag="ACHGNW_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN6926840.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3327..4100)
                     /locus_tag="ACHGNW_002582"
     CDS             complement(3327..4100)
                     /locus_tag="ACHGNW_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926841.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4110..4487)
                     /locus_tag="ACHGNW_002583"
     CDS             complement(4110..4487)
                     /locus_tag="ACHGNW_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6926842.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4508..4822)
                     /locus_tag="ACHGNW_002584"
     CDS             complement(4508..4822)
                     /locus_tag="ACHGNW_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6926843.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5022..>5583)
                     /locus_tag="ACHGNW_002585"
     CDS             complement(5022..>5583)
                     /locus_tag="ACHGNW_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN6926844.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1653 a         1100 c          940 g         1890 t
ORIGIN      
        1 tacttcctat tttatcatga aaaatatctg ttaagcacca atgattcggt taaataagtt
       61 taataacaca tctttcacgc cagctgcatt gaacacaagt ccgacggcga ttagagcaat
      121 aatcaagaag ccaatgagtt ttgaaaattc tcgtttaaaa cctaaaaaca gtccaatgac
      181 aacaatcgct aacaatacca atgactgtgc attatccaag aaccagttgt acaaattctg
      241 cccaaaattc atgtgattgt cctcctttcc ttaagatatt tttctcgata atagatatag
      301 ctttttacac gccttaataa ttgaaaaggg gttaaatgct ctccatagac ggttactcga
      361 ttggtataat tattataaaa tctcaccttg taaaaatcat aggtatagat ggtatcacca
      421 gcttgccgtt ttccctcaat aatgagagac gtatatttcc ctgtattttg tatatacgct
      481 tgaaactcaa tgccacactg aaaccctaac ttctttaatt cttttgttaa aaggctcatg
      541 taatcaaatc ctccatggct agatgttgtt gttcaattaa tttctcatgt ttttctgtca
      601 gtttggcttc ctgaatcatg gtggagataa tggttgtacc gttgagaaca tctaaaatgc
      661 ttgcgatttt taatgtaggg gcgacttgat gatgtaacca gttcaatgtt cgttcaaaag
      721 aataaggctc tggctgtgtg gttaggcgta atgctccacg atttttaccg ataaaccatt
      781 cccaacgttc attagttttc caatcactcc gacgtttagt gtcgtcttta tctgcaaagc
      841 ggatatagcg attgataatt tcaaaggctg ttttttccgc ctgttgatgt gccaataaat
      901 ctttcatggc ttgggtggct cggtcatttt ttaaccgaat ttcaaaccga ttctttactt
      961 ctgcgtcttc taagggaaca tcatttttca cgtattgctc ataatctttc tcatatatac
     1021 agaaatagac ttcacttttt aatgagccaa tataaagtgt gtttcccatg tatggttttt
     1081 catcacggtg aaccaattca ccactgcgat aatttttaaa gcttcgaaag acggaaatgc
     1141 attcttcctg tttacatttc tttgccaaat ctggaatatt caaaatccct gttttatcgt
     1201 tgatagctaa atccaagcgt ttgaaaatac ctttatgttc catgcaatct tgaaagaaat
     1261 cataccagct ccgatgttga gccaatagaa agttttcaaa ttgccgacag ccttttcctt
     1321 tcagttctaa taaaacacct ttacttacat catgagagac taggacaaca atatcaccga
     1381 aacgatagtg ttcgggataa gaataatagc caaagtcttc gtgtagcata taatccatat
     1441 tcaaacgtaa aacctcaccg ataatttgag ccacatcatt ggtagggaat cgaatccgta
     1501 catagtcgaa taacatctcc aatgggttat cgggattgaa gcgttccaaa gcgttcagta
     1561 aatcttcttt cacctgttga gtagggactg ccttgcctgt ttcaatatcc gataggtaag
     1621 gtctggtaat atgagaagca accgccagtt tattttgaga aacgccataa gccagtcgct
     1681 tttctttcaa ttccttaatc caaatttttt cgttttccat tcgcaaacct ccaatctaaa
     1741 aaagatgtaa actatgaccc ttgagtttac acctttcata ttttccgcaa tcctttgtac
     1801 catcaacatt ggttgatgag aattgcctgt tttcagttgt atttgtgccc ccctgttaga
     1861 tagagggggc taaaggattg gcgtggctaa cgccacacca atcaaagcaa gtctgaacct
     1921 gtacctttca cttcgtgcgg tacaggctca gattgctttt tttgaatgac ttttcctatt
     1981 tctcctaaga aatcgtacct ttttggtaca aggggtgtat aaaactcact aatcacactt
     2041 tttcctgtat ccacgtaacc tcttcccttg atgggtttta agaaaaattg tttttgtgta
     2101 tcgcttccga acatcatacc aaagcccatt tcagacatac gacccaaagc cacacggaag
     2161 ttaaagttgt cccgaatccc atcactaaaa tattttgcgt ctggacgttg acaggcaaga
     2221 attaaaaaga agcctgcttg tcgtcctaac attacaattt gtttgagttt atgaataacg
     2281 gtggtacttt cacgacctag catatccatc attgccacat attcatcaaa aataagaaaa
     2341 ttggcagaca aaccaagata agcataattt tctcctgttt tatagttagg gtgttgtttc
     2401 atggtttcac tgcgatcaat catatcttcg taaaattgat tgatacatgc caacatatct
     2461 tctttacggt aatagacatt attcatgacc gcccctaagt ctgccaaatc agcattttta
     2521 gggtcaagaa tataaagctt tgcatttgta tgcaggagag attcaatcaa tgtgagaata
     2581 aaataggatt ttccactacc agtgccccca gcaattaaca tgtgagggag agaatcgaac
     2641 tcccaataga ccgattccat caatttgaca cgtccatctt gagctaccac gtcttcaatg
     2701 ccaattcgat tgccaatagt atcataaagt aggacatact ctacataaga atctttcaac
     2761 gttttttcca ctaattcaca atacaatcct gtttctagtt tgttttctag gtgcaaaagc
     2821 tgatcttgat atttccctaa ggtgatttct acatgaatat ggattagtcc atctttcatt
     2881 cgataatata ttttaggaaa atggcggatt cgttgtttgg ttttcttgct gggtaaatct
     2941 ttaaagaatc cctctgattg agtttcttcg gcttcatacc aatgattttc taaaatcata
     3001 cgagctaact tttgacggtg ctctaactgt tttatagaat ctatacgata gtgataataa
     3061 agccagctga taagtccgca tacaaaaaga gcagtgagaa tactgctcca aaagtatggc
     3121 gtatgaagtt gatagaaaaa tgtatctaac tgaactgctt gccagttcac cgattgtaac
     3181 gctctccaat gaaaagggag taggacgact agaaaaacta ccagcaatga aattagtaca
     3241 aaatgataga ccaagtgctt atcgttggct ttgattcgct ctcctctgtc atgaagcaat
     3301 ggcataaaaa tcctcctttc cacctatcaa aataattgtg tattgtagat acgagcctgt
     3361 ttatcaaaag ttggttgaga aatggcttca tatttctgat aagcgattcg cttttcaaag
     3421 cgtaaatttt tttctttcgg ataataatga cgtcgataga gagtgtgtcc taacagtttt
     3481 ttcgcttctt ttgtcaacgg atagagatag cgaaacatgc gtccattgat tttctcaatc
     3541 cctttgtatt cgcaaaatgc ctgggaaagc caatgctttt tctctacctg ttcaaagcga
     3601 gcattttcct ctaataaaag gcgagcactt cgagggtgta tcttttccca tgactgctta
     3661 tcacgataga cacttgtctt gaaatacccg cagtaaaaga agttggacgc ttgatagaca
     3721 tagccacatt ttccctcaat tccatctgcc agtgtatata aaaaatgaca gtccgtatgt
     3781 tctttcaacc acttaattag agctgaaaga gcttgactgc caaaataatt ggtttgattc
     3841 atttctggaa gaaaacacat ctttccaatt tctaaataat ctgtggtttg aagggaactg
     3901 tcgggaaaga gtttgcgtat ggtttgtagg ggttgcgtac cccagcctaa ttccacaacg
     3961 cctaataatt tttcttctga aaaaatccct agaaagtatt tacacaatct agggagtatc
     4021 tttgaataat ggtattgccg aataaaagaa atggcttcat ccttcggaat ttctttcaaa
     4081 atcaatggtg tgtttgtcat aagcaacacc tatttttcac tttcggacgc ttctttttta
     4141 tatttctgat gaggttttgt ctgtcctttt aatacaagat cgtctgcttt cacgtaccaa
     4201 tcaacgtctg cccctcgaaa agtagccgtc gctacggtat ctgccacagg attgattaat
     4261 tccacttctg cgttgtagtc aaattctttc aaaggaacag aagcggggat actgacttgt
     4321 atcatgcgtc cttgtccttt tgattttaag tcataggtac gttctttaat ttcttcggaa
     4381 accgtaccat cttcattctg gatacgaacc tcacgacgca aggcggaaaa tttcaatgtg
     4441 ccaaatgtct ggtctttttc aatcacaata ccttctgcta atctcatatt ttttcacctt
     4501 tcctttctta tgctttcacc atatcgtctg cgtgcataat gtaattggta aatcctcgat
     4561 tgccaatttt ataaccttct gcggtaatat gtgggttgat gagtttgact ttttcgtcgg
     4621 attcaaaatg tttctcgcca gcttcggagg gaagaatgac aacaatatca tctgctcgtt
     4681 gaatcgttga gtataaattg taactacgag agagaatggt tggtctgcca tttattcgac
     4741 gttgttcaat gttcccttca ccagcatatt ctaattgtcc aaatgttttt tccatattgg
     4801 gaatgacata tttaagttcc atatttttac ctatcctttc tgtttttatg tttgaatgaa
     4861 tcttatttgt gtgatggttt taaagggggg agagcgacct tttgattctt gattttttat
     4921 tttcataagt tcacttcctt tcaaaattgg gtaaaaaaat agacgctccg tactgagcgt
     4981 ccgattccta ctatatttaa aacaacgact acctaggtat tttaaatatt ataatcaaat
     5041 tctattttat ctaataattg agtgggataa agcaagtctc ctttactcgt aaaataaccc
     5101 acctctgaga aaagaaataa attatttttt gctcttgaac aaattacgta tagtagtttt
     5161 ctcgctgaac taattccttt atcagaaaca ttaaaaattt cattccaatg aggaattttc
     5221 ccgtttagaa gattaatagc gataacagta tcatactcat ttcctttaac tttatgtgct
     5281 gtagtaagtg tgataccatc tcgagattta aacatcttct taaaactaga tacactacta
     5341 gataatgaat tattttttat ccgatcttct acttcgttaa taaaaaatag atagtgctta
     5401 aaaatagacg ggtaattttt ctcgtcaata ttaagattaa caaatatatg tttggtaaca
     5461 aatttgtata aatcaacacc gttgtcgtct ccactataca ttcttttcac actgtttatt
     5521 agctgtaata aatctaagtc aataaaataa tctttaactt ctattccaaa ttcatttttt
     5581 aat
//
LOCUS       DAZBTH010000141         5560 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000141 DAZBTH010000000
VERSION     DAZBTH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.146"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACHGNW_002586"
     CDS             complement(60..2009)
                     /locus_tag="ACHGNW_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926845.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACHGNW_002587"
     CDS             complement(2051..3109)
                     /locus_tag="ACHGNW_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFN6926846.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACHGNW_002588"
     CDS             complement(3228..3683)
                     /locus_tag="ACHGNW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926847.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACHGNW_002589"
     CDS             complement(4267..5070)
                     /locus_tag="ACHGNW_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6926848.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1632 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga
//
LOCUS       DAZBTH010000142         5534 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000142 DAZBTH010000000
VERSION     DAZBTH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.147"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACHGNW_002590"
     CDS             complement(<1..988)
                     /locus_tag="ACHGNW_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFN6926849.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_002591"
     CDS             1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN6926850.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ACHGNW_002592"
     CDS             1813..2967
                     /locus_tag="ACHGNW_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFN6926851.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ACHGNW_002593"
     CDS             3182..4150
                     /locus_tag="ACHGNW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN6926852.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ACHGNW_002594"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ACHGNW_002594"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN6926853.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="ACHGNW_002595"
     CDS             5180..>5534
                     /locus_tag="ACHGNW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFN6926854.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DAZBTH010000143         5466 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000143 DAZBTH010000000
VERSION     DAZBTH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.148"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACHGNW_002596"
     CDS             complement(<1..447)
                     /locus_tag="ACHGNW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6926855.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSC"
     gene            complement(460..1791)
                     /locus_tag="ACHGNW_002597"
     CDS             complement(460..1791)
                     /locus_tag="ACHGNW_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN6926856.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1802..3244)
                     /locus_tag="ACHGNW_002598"
     CDS             complement(1802..3244)
                     /locus_tag="ACHGNW_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6926857.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3231..3995)
                     /locus_tag="ACHGNW_002599"
     CDS             complement(3231..3995)
                     /locus_tag="ACHGNW_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN6926858.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4099..4974)
                     /locus_tag="ACHGNW_002600"
     CDS             complement(4099..4974)
                     /locus_tag="ACHGNW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6926859.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5229..5313)
                     /locus_tag="ACHGNW_002601"
     tRNA            complement(5229..5313)
                     /locus_tag="ACHGNW_002601"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5277..5279),aa:Leu,seq:caa)
     gene            complement(5331..5402)
                     /locus_tag="ACHGNW_002602"
     tRNA            complement(5331..5402)
                     /locus_tag="ACHGNW_002602"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5368..5370),aa:Gln,seq:ttg)
BASE COUNT         1655 a         1161 c          831 g         1819 t
ORIGIN      
        1 acaactatct ttaatcttta cttcttggtt ttgttttaat aaatttcctt caaaaatgcc
       61 tggagaaact acaatgatca ccgtatcttt ttgtgcttgc tccaacttct tcaaatctat
      121 aatagctaac agtgtccctt cttttacctc ctggttactt tcaacccgta tctgaaacgg
      181 acttccttcg agttcaactg tatctaaacc catatggatc agtatttttc tgccagattc
      241 actttcgatc gttatggcat gtcgggtagg aaatatatta tcgattattc ctttaactgg
      301 cgcatggata tatccatcat gattcgttat agcgtatcca actcctatca tttttgaaga
      361 aaagacctcg tcttttactg ctgttaatgg aataatttga ccactagcta cagcttttag
      421 aatgtttctt gttttaaaga aatgcacttt ttatcacctc tactttaatg ctggccaata
      481 ttttttgttt actttgatca tttcatctag tatttttttt gcgactctgg catttggtac
      541 ggtttgattc aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa agtaagcatc
      601 tactagtaac ttctctactg caacttgtgc ctccatcaat cctttatgaa aatcactgat
      661 tggttttctt agtgtaattg gctccacacc agttgcacca acatatgccg gtatttcaac
      721 aactgcatct gaccgtagat tcggaatcgc cccttgatta ggaacgatta gcataaagcg
      781 acgacgatca tcattcaaca aggaaatagc tatatcgacg atatattgtc catgctcccc
      841 aaaattaaag ttcaatactt cttccacaat accgcggcgt attttatcag ccatttcttt
      901 ggtatttttc tctcgtccat ccatgaccat attagcacgt gtatactctt tgtcagtatg
      961 ctcaacgacc atatcaggat ataagtaata ctctaagtaa ttattcggga tattacttgg
     1021 aaaatttttc gtgatgactg acatcatttg aaaggttttt tgccaagttt tatctcctat
     1081 attaaaatcg gcgacttgca tttcttttgt tattagtttt tcaataatct ctggcataat
     1141 ttctcgttgc aattctttat cataaatcga tgtataccag ccaaagtggt ttaaaccata
     1201 atacgtaacg atccaattct tacgatcata accgtaattc actgcaatcg tttcttcaat
     1261 tgaaatagtc atatcacagg cattaatcat ttttacattc ggatatttac gacgaacagc
     1321 ttcagaaacg atcgactctg gatttgtata attcaaaatc catgctttgg gggcatagtc
     1381 ttgtatatga tcgacaattt ccaataaacc tttgattgag cgcatcccat aagcaaatcc
     1441 tccgagtcca cacgtttctt gtcctactaa tccatgttta agtggaatct tttcgtcctt
     1501 ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc atcctgtaaa
     1561 agcaagcttt ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt ttcttttcag
     1621 cataaattta acaatcaagt acatatccat atttctttct tcatcaatat catataaacg
     1681 tatttcagat aatggcaacc gtttttcatt gttcaaaatg gcttgaacga ttcctggtgt
     1741 atacgtactc ccgcctccag ctatagtgat aatctgtttt ttcatcatca tcttttcctt
     1801 tctattcttc taataattta ttgaattctg ccataacata agtcacttct aagccgatga
     1861 taatttgata atttttttca gttggtttaa tgatcccgct tatccctaat tgcttgattc
     1921 gctgttcctc gaccatggtg ggatccacta cctctacccg taggcgtgta ttacaactat
     1981 agacatccag gatattggct tttcctccaa acgcttgtag catttttgtt gccatatatt
     2041 cataattttt acttgctaac cctaactctg tttcttctaa ggttacttca ttgccaaact
     2101 ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa taataaaaag
     2161 taacgtaata caaggcaaaa aagaaaattc cgattggaag aatcaaaata gcattggtag
     2221 cgattcgaaa attgatgaga taatcaacaa tacatgctcc gaatgtaaaa ccaattcgta
     2281 tattgaataa gtaacagaca atccctgcta aaccggcaaa aagtgcatgg atcacgtaca
     2341 acataggaga aagaaacatg aaactaaatt caacaggctc agtgatattt gcaataaatg
     2401 aagttcctgc accgcttccc atgattcctt tgaccatctc tttattttct ttataagctg
     2461 ctttatagat agctagacat atagctggta taccaaacat cattactaca aaaaaaactg
     2521 ataaaaacag tccagcctga ggatctccgt tgataaagcg tgtcatctct cctctgaaaa
     2581 cttccttgct aggagatgta tagcttccta aatcgtaata aatatacgta ttcaacacat
     2641 ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat aaatatcccc acacctaatg
     2701 ctcccatatc aacgagcagt tttgcaaaat tatcaatccc aatttggatg agcggccaga
     2761 taaagcccaa aaaaacagct gcgattgttt gtagaatcat caccatagtc aatgggaatt
     2821 tttcgcccgc aaaataagaa aaaactactg gtaatttttg ttctttaaag cgattatacg
     2881 tccatgccgc tactaaaccg gaaataattc caccaaaaat ccccatattc acttctgggt
     2941 ccataatttc taagcctttt ttcaaggtag caatcgctaa aaaaccagta agtgcaggta
     3001 ttcctttgtc cttatttttg acaaaaccga ttgcaactcc tatagcaaat aagatatcca
     3061 agtttccaaa aacggcattt ccagctgcac caattactgg gatgttcagc atatcgtcag
     3121 ctgataaacg catgatcaat cccgctaatg gcatcgctgc aactggaagt agcatcgctc
     3181 ttccaagctt ctgcacattt gatttaacat tcatttttat tcctcctcta ttaaatatcc
     3241 tcatatgtta ctaactcaat caaaggatca tttttcactt tttgagcaaa tgctgaatga
     3301 attaaaaatt cgacttggtg ggtacgagga tacacaaaac ttgaaccatt taacaattcc
     3361 tgatccaaat acgctgcgtg acacatgact tcgatcgttt tatcttctct caatatcatg
     3421 agtaaacttg ccaaatattc ttccacactc gtattgattt gtcgtttttt ttcatcagtt
     3481 tctcctacga catcgaaaca aggctcaaaa taatcgacat gacgaacttc atcttttcgt
     3541 tcaaagttgc tccgaaccgg caaatcatat ttttttgcta atgtaacaac tacctcctga
     3601 ttttcatcca aagtatgagt atgatgatga ctgtctaaat gagttggtat gatccccgca
     3661 tgataaattt tttgaatctg agcattccat tcctgataca gttgatcttt gtcaatgtta
     3721 aatggatgct gataataaga taaggatctg aactctccgc tttcgataag agtatcgaca
     3781 gttttgagga gtggtttgcc acacgtcaat gttaaatgga ctcctacacc tagcctaggc
     3841 atttcctttc ttagtttcac agcatgctcg aaacccggca tatttgccat caatgtagtt
     3901 gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat aattcacacc atggctatag
     3961 ccaaaatcat ccgaattaaa aatcactttt cccatttcat tctccttctt ttttctacta
     4021 aaataggcgc aaccaaataa tcatagtcca tttactatgt gtcatttggt tgcgcctata
     4081 gatagttacg cccttatttt aatagattct tgctagatga attgccaaat agatgatttc
     4141 attttcttga atggcaagtt gaaattgctg ttcgatgata tctttaatct ttagtgctgt
     4201 acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt ctactggcgt
     4261 ttcttcctga taaatcaagc gtttaaccag gaatttgata tgaaccatta agcgatgata
     4321 agtcagtgat tctttatcat aaacgtttga ataggatttt tctagatggg acaaaatcgt
     4381 ggtcgtcatt tctactattt ttaagctctt taaactatta ggctctccta gctcagcatt
     4441 aatgatatgc atagtgatga atcctacttc atttgaccct aactcggttt gatatcgatg
     4501 attgattcgc tcgaccactt ctactgctat atcatattca tttggataaa ggaatctcat
     4561 ttctggcaac ataattgctg tgacatctct atcttctctt tttcgttgaa tagcaaaata
     4621 aatatgatct gtcaatgtaa tatagatatt tgaatctaat ggagaggaga tcctagctgt
     4681 tatcagttca atcacttctt ccgcaaaatc cataaattcg attggaatct gcgagactat
     4741 ctcactgaat cgtttgatct tttctttaga atctaataca aataatttgt cagcatcctc
     4801 aacatcaaat ggcttactca ctttagcaga aaagccaatc cctttcccca tcgcaatttt
     4861 ttctattcca tcgtcatcga tcaatacagc attatgattc agctttttca tcacatacac
     4921 cgttaattca cctcctctat atgataaaac aaaacaactt gcagactcct tcatacatat
     4981 ttttgtctcc aaacaatttt taaagacaaa aaacgaccta agaaagaacc ctttcttagg
     5041 tcgtgcctaa ttgaatagta gcaccctaca taatttacaa aaatagttta acacataata
     5101 aaagcgttat caatatcttt tttactcgtt ttatgataat acttgtatcg aattgatttg
     5161 gaggtaatta cataaaaaca aaaaaaatat agcaactctc tataagaaag ttgctatatt
     5221 gataattata ccggcggccg gggtcgaacc ggcacgtcct caacggacac tggattttga
     5281 gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta aaaataataa ctggggtagc
     5341 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
     5401 caagaataaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     5461 cgttaa
//
LOCUS       DAZBTH010000144         5152 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000144 DAZBTH010000000
VERSION     DAZBTH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.149"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHGNW_002603"
     CDS             <1..649
                     /locus_tag="ACHGNW_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926860.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHGNW_002604"
     CDS             708..1148
                     /locus_tag="ACHGNW_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926861.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHGNW_002605"
     CDS             1160..1828
                     /locus_tag="ACHGNW_002605"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6926862.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHGNW_002606"
     CDS             1892..2515
                     /locus_tag="ACHGNW_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN6926863.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHGNW_002607"
     CDS             2571..2957
                     /locus_tag="ACHGNW_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN6926864.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHGNW_002608"
     CDS             3170..4561
                     /locus_tag="ACHGNW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN6926865.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          861 c         1084 g         1500 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac ag
//
LOCUS       DAZBTH010000145         4976 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000145 DAZBTH010000000
VERSION     DAZBTH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.150"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            864..1775
                     /locus_tag="ACHGNW_002609"
     CDS             864..1775
                     /locus_tag="ACHGNW_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFN6926866.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2262..2528
                     /locus_tag="ACHGNW_002610"
     CDS             2262..2528
                     /locus_tag="ACHGNW_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926867.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2600..2941
                     /locus_tag="ACHGNW_002611"
     CDS             2600..2941
                     /locus_tag="ACHGNW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926868.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2996..3415
                     /gene="ssb"
                     /locus_tag="ACHGNW_002612"
     CDS             2996..3415
                     /gene="ssb"
                     /locus_tag="ACHGNW_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6926869.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3492..3788
                     /locus_tag="ACHGNW_002613"
     CDS             3492..3788
                     /locus_tag="ACHGNW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926870.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3803..4234
                     /locus_tag="ACHGNW_002614"
     CDS             3803..4234
                     /locus_tag="ACHGNW_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN6926871.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4539..4721
                     /locus_tag="ACHGNW_002615"
     CDS             4539..4721
                     /locus_tag="ACHGNW_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926872.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1836 a          763 c         1037 g         1340 t
ORIGIN      
        1 ttttgaaaca aattcactca ttttttgagc aaaaatgttt caaaatttga acaaaaacca
       61 attgaaaatt gaagcaaatt tcactcgaat tgaatcgatt ttgagctaaa atcaatagaa
      121 aaaataaacg tattttgttt gaaatccgtt tatttttttt acgtttcagt attagtaagg
      181 attatttgag ggttatttag agattattta aaggttatat gtaataagtg gggggagggc
      241 tgtcggcgct tggtatgaaa ggcattctcc gacttccagg tttttgacca ttttctctca
      301 acgtggaaaa accgccactt atgagagaaa atggtcaaaa acctaagcgg gaagtctccg
      361 aataacatac caagcgccga cagccaacag ataaagaata cactactaaa aatagtagta
      421 gcataaaatt taaaagggaa aaaatcaacc cctaccccag tcttggggtc tcaaaactaa
      481 ctccccccta accccctctt tcagcctctt ttgaaagagg gggagaatca accccacggc
      541 caacgcccca gtgttcttgg ggctgtccag aacagaaact tgcgagagag tgggggtaaa
      601 acaagtgtag aggaaacttg aagacttgtt cgaacagaaa cttgcgagag agccggagta
      661 aaacaagtgt agaggaaact tgaagacttg ttcgaacaga aacttgcgag agagttggag
      721 taaaacaagt gtagaggaaa cttgaagact tgttcgaaca caaacttaca agagagggga
      781 gtaaagcagt tgcagaggaa tcaggaagat ttgttcgaac agaaacttgc aagaaagcat
      841 tattaaattg aaggtgatga aatatgaagt atttattgca tggctatttt attgattatt
      901 ctactcgcaa tagtagtact ttagcgattc tatgtttgct tcatgaattg agagtggtta
      961 ctagaaaaca gctatacaaa ctattgaatt ttgacagatc aattagtaaa caaggagtaa
     1021 cgaatgcttt agtgagatta tatgaaaaac agttaatcga tcgaaaaaaa tatggaacag
     1081 aaatgtatta ttatttgacg aaagagggac atcaaagcat aggtgggtat tatacgttgc
     1141 cgaaagttcc tgaatataat ttgaatcacc atttacaaat taacaattat ttgattaaag
     1201 gaatggcatt ggcaaaagac cacccacatt taaaacggat tatttcagaa agacgtaccg
     1261 tctatgaaac gaaggattta aatcctaaga caaaaggaag aaaatttttt gtgccagatt
     1321 ttatttttcg ttttcgagac aaagaaaatc atgagatcaa ttggtccttt gaaattgagc
     1381 taacgttgaa aacacgtcgt cgctaccgtg aagcgatttt tccgaaatat attcgtgagt
     1441 tgcgaacacg tcctgaatca catttaattt atgtgacacc tagtggcgca attcaagaag
     1501 aacttcatgc gtttaagcgt atttttttca agcgcgaaac tgaaaaagat gcgagtcaac
     1561 aagaattgtt tgagcggttg catatttttt cagctttaga gtttgaagag ggattaaaag
     1621 cattattaga gaatgatcca gcagtcaatt ggcggacagt agaacgagaa aaaatttatc
     1681 cgaatggcgc tgttcaaagt cgtttgaatt tatttgaaga atcaactgaa aatgaggaaa
     1741 aaccaaaagt gaagtttacg cctgaagtat tgtaaaagag ggtgttcgct tcgctctttt
     1801 tttgtgccag tttgctggca ccgttcagac gcaagcgtct gaaccggctc gtttttagga
     1861 aggactaact tgtttgattt atccgttgtc aaagaagtgg acgttcgaga gtccctaatc
     1921 gtatagcagt cattcggcga gtataaccta aaacataacc tgccaattgc caagggtaaa
     1981 atgcacgctg cgcgcccttg gcaatcgtca gaacatgttt tcgagccggt tatgccgaac
     2041 gaccgctata cgaaaagggc ctctaaacag aagtccactt tgatccttga caactgaaac
     2101 ccaaaatcaa acaagcaggc cttcgtctaa aaacgagccg aatgaagacc ttgggggttg
     2161 cacccccaaa cccccgtgcc cccgacacac taccgaaaag gtgggggtgg gtggcgaaaa
     2221 aatcaaataa aacattctat tcaagaaagg aagaaacaac catgacaact aaaaacgagc
     2281 aactgaaaga acaggcaaga aaagagttgc aagaaagagg tttgattatt gaggggagct
     2341 ttgaaggaga ttttgaaacc tatattggtt gttatgcaag accgataaac aaacctacag
     2401 ccttagaccc aatgaacgaa caagaagtaa aagaacaaga aaaacatgca gtgaatggtt
     2461 ttccgcaaga tttcactgaa tggtatgagt gggaaattaa caacgggaag cttgaaaact
     2521 tcttataaaa gtcagatgac gtgcaaagag aatgattctc tttgtacggt aaaaatataa
     2581 agaaagaagg aaaaagaaaa tggcaaaaaa cacaccttat caagtaggag acatttttta
     2641 tagtagttgg ggctacgaac aaacgaacgt gactttttgg caaattgtga agttaacaga
     2701 aaaaacagca tggtttcagc ctattcaaaa gaaaacggtc gagcattatt cgtccatgag
     2761 tggaaaagtt gttccaatga aagacaagtt tatagagtat cctttagcaa aaactaaaga
     2821 cagtattgta aaaaaacgaa ttgatccaga aacctatcct gatatgttat gtggttatcg
     2881 tagttgggac acgtttcata gatataaaaa tcaagctgtt tatgaaagta gttattatta
     2941 aggggatagt ttcccctttc aaaaataata aatagaagaa tggagagaaa aaatcatgat
     3001 gaattcagtg aatttagtag gacggttaac aaaagaagtg gatttaaaat acacaccaaa
     3061 aggcaatgcg acaggcacat ttattttagc tgttaatcga aattatacga atgcaaacgg
     3121 cgagcgtgaa gcagattata tccgctgtgt catttggcgg aaagctgcgg aaactttagc
     3181 aaaatatact ggaaaaggaa gtttgattgg cattaatgga cgtcttcaaa cgaggagcta
     3241 tcagaatcag caaggacaga ccatgtatgt tacggaagtt ctagtgacag atttctattt
     3301 gttagagagt aaagaaatca atgagcaacg gtcaaaatca atggcgaata atcaaaataa
     3361 ttcttcaatt ccaggaaatg agattcaaat ttcagatagt tatttgccct tttaagtgaa
     3421 aaataatttt gttctatacc tgatttcttc aggtatagaa caaaaaataa atagagatga
     3481 ggcgattttt tatgaaaatc attgacagtt acgatcaacg aaccatgcaa tatttgggct
     3541 cgtttgatta tacagatgaa aatattatta attatgttgc aagtttacct ccttatcaaa
     3601 gcgttcgctt ggtagatttt gcaagtgatg aattggtatt aacaacgatg ggcaactttt
     3661 tagattatgt cggagacaaa aaatggttgg aaaaaatttt gtctaaattg attgcgaaac
     3721 aaacaggaga agaacccata aatgcgattc aattgttcaa tcgatatgaa caagaaaaca
     3781 atgattaaag aggagagaaa aaatgaaaag attaaaacga agtattttta ttgtgctagc
     3841 attgattgat tttgttggga caggttgtga agcaattgac gttcaaaaag ctcccttatc
     3901 ggaaataaaa caagcaataa caaaacaaga tgaagaacaa ccgctaaata cacaagtaga
     3961 aatagtgaat caaaataaac cgagtttttc tgaagaagat ttttcgttag aaaatggaac
     4021 caggcaattt ttttcagatt tggatcgttt aaatcgggta ggtgtagcaa gtgcactttt
     4081 gcataaaagc aggatgccaa cagaagaacg agaccgatta tacattaagc caactggctg
     4141 gaaacaaaag aaaatgaaga atggcgatta tctttataat cgttgtcatt tgattggcta
     4201 tcaacgtgcg acaagtttcc ccataagtat gtaatacgaa acgggaggat taatcttaag
     4261 attaatcaca actaacctat ttgacagttg gaatgaaagc tatcatgtct gctgaatcaa
     4321 ctggtctgat actcctacat gcaataaagt ctactaggaa gttattgtat gaagctaggt
     4381 aaaggcggca gaacaatttc taagtgtgga atcatatgaa gttggatatg ccgggacgct
     4441 ataaattact cagggtgaga ctgtttttaa ttaaactgac gaacttgcga atgtacgggt
     4501 ctataaccga cttatattcg aaagatataa gattgcgaat gcaaagaaca ttatcgaaaa
     4561 gtaagaatgt tggtcatgaa attcgagatg gaagactagc ggtgtatctg tcaagagttg
     4621 gtaaaacatg tttgattgat tcagatgaga gtgggaatgc gacaaaacgt tttacagaag
     4681 aaatagagca actagcagaa ctgaatatac gatattttta aatactttag cggagaataa
     4741 aaaaatcctc tacagaaaac tacggagggc aacatgtgct attgtttcaa caatacattt
     4801 ttaattttta ccataacaaa aaagtacttg aaaaaaaaca gaaatatgtt aaattaaaaa
     4861 tgaaaaaatg gaagcggtta cttattcttt tgaatctaag ataaaaattc aaaagaataa
     4921 cataacccaa atatgattat acttgagatt ctaaatattt atcgaaaagg aatttg
//
LOCUS       DAZBTH010000146         4908 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000146 DAZBTH010000000
VERSION     DAZBTH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.151"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHGNW_002616"
     CDS             complement(207..1169)
                     /locus_tag="ACHGNW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6926873.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHGNW_002617"
     CDS             complement(1171..2610)
                     /locus_tag="ACHGNW_002617"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN6926874.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHGNW_002618"
     CDS             2795..4741
                     /locus_tag="ACHGNW_002618"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN6926875.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1585 a          827 c          798 g         1698 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagtt
//
LOCUS       DAZBTH010000147         4788 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000147 DAZBTH010000000
VERSION     DAZBTH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.152"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHGNW_002619"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHGNW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFN6926876.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHGNW_002620"
     CDS             1019..1183
                     /locus_tag="ACHGNW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926877.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHGNW_002621"
     CDS             2141..3118
                     /locus_tag="ACHGNW_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6926878.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHGNW_002622"
     CDS             3128..3625
                     /locus_tag="ACHGNW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6926879.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHGNW_002623"
     CDS             4001..4282
                     /locus_tag="ACHGNW_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926880.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHGNW_002624"
     CDS             4284..4700
                     /locus_tag="ACHGNW_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926881.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZBTH010000148         4754 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000148 DAZBTH010000000
VERSION     DAZBTH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.153"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACHGNW_002625"
     CDS             72..1118
                     /locus_tag="ACHGNW_002625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6926882.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACHGNW_002626"
     CDS             1135..2358
                     /locus_tag="ACHGNW_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6926883.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACHGNW_002627"
     CDS             2447..3334
                     /locus_tag="ACHGNW_002627"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6926884.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACHGNW_002628"
     CDS             3420..3650
                     /locus_tag="ACHGNW_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN6926885.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACHGNW_002629"
     CDS             complement(3707..4672)
                     /locus_tag="ACHGNW_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6926886.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1606 a          790 c          967 g         1391 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttct
//
LOCUS       DAZBTH010000149         4720 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000149 DAZBTH010000000
VERSION     DAZBTH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.154"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHGNW_002630"
     CDS             complement(<1..1097)
                     /locus_tag="ACHGNW_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6926887.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACHGNW_002631"
     CDS             complement(1227..2603)
                     /locus_tag="ACHGNW_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926888.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACHGNW_002632"
     CDS             complement(2603..3259)
                     /locus_tag="ACHGNW_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6926889.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="ACHGNW_002633"
     CDS             complement(3269..4546)
                     /locus_tag="ACHGNW_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6926890.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="ACHGNW_002634"
     CDS             complement(4565..>4720)
                     /locus_tag="ACHGNW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN6926891.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DAZBTH010000150         4712 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000150 DAZBTH010000000
VERSION     DAZBTH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.155"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..358
                     /locus_tag="ACHGNW_002635"
                     /pseudo
     CDS             107..358
                     /locus_tag="ACHGNW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="ACHGNW_002636"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="ACHGNW_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN6926892.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="ACHGNW_002637"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="ACHGNW_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1787..1870
                     /locus_tag="ACHGNW_002638"
     CDS             1787..1870
                     /locus_tag="ACHGNW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN6926893.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="ACHGNW_002639"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="ACHGNW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6926894.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="ACHGNW_002640"
     CDS             complement(2995..3858)
                     /locus_tag="ACHGNW_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6926895.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="ACHGNW_002641"
     CDS             complement(3860..4132)
                     /locus_tag="ACHGNW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6926896.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4364)
                     /locus_tag="ACHGNW_002642"
     CDS             complement(4149..4364)
                     /locus_tag="ACHGNW_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN6926897.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4456..>4712)
                     /locus_tag="ACHGNW_002643"
     CDS             complement(4456..>4712)
                     /locus_tag="ACHGNW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFN6926898.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct caagcatttc
     4501 aaaaaatacg ttcttataca tggataaaac ttttttgtca tagcctttat gctctgtaat
     4561 gccgttttta gaccaggtac ttactttatt acttcgcttg ataatatttt ggaaaaccaa
     4621 gttatcttct ttatgttgct tatacagttc ttccaggttt gattttatcg ttgcgctgtc
     4681 cgtatcaact aaataaggaa caaaaccgat ca
//
LOCUS       DAZBTH010000151         4646 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000151 DAZBTH010000000
VERSION     DAZBTH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.156"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACHGNW_002644"
     CDS             complement(133..717)
                     /locus_tag="ACHGNW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926899.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACHGNW_002645"
     CDS             complement(928..1131)
                     /locus_tag="ACHGNW_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926900.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACHGNW_002646"
     CDS             complement(1141..1563)
                     /locus_tag="ACHGNW_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926901.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACHGNW_002647"
     CDS             1801..2415
                     /locus_tag="ACHGNW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6926902.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACHGNW_002648"
     CDS             complement(2580..3404)
                     /locus_tag="ACHGNW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926903.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACHGNW_002649"
     CDS             complement(3428..3583)
                     /locus_tag="ACHGNW_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926904.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACHGNW_002650"
     CDS             complement(4042..4299)
                     /locus_tag="ACHGNW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6926905.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACHGNW_002651"
     CDS             complement(4292..4561)
                     /locus_tag="ACHGNW_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6926906.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAZBTH010000152         4611 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000152 DAZBTH010000000
VERSION     DAZBTH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.157"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACHGNW_002652"
     CDS             complement(86..400)
                     /locus_tag="ACHGNW_002652"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6926907.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACHGNW_002653"
     CDS             complement(451..705)
                     /locus_tag="ACHGNW_002653"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6926908.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHGNW_002654"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHGNW_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN6926909.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACHGNW_002655"
     CDS             complement(1145..2098)
                     /locus_tag="ACHGNW_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926910.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACHGNW_002656"
     CDS             2710..4203
                     /locus_tag="ACHGNW_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN6926911.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACHGNW_002657"
     CDS             4338..>4611
                     /locus_tag="ACHGNW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN6926912.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAZBTH010000153         4507 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000153 DAZBTH010000000
VERSION     DAZBTH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.158"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..960)
                     /gene="lacD"
                     /locus_tag="ACHGNW_002658"
     CDS             complement(<1..960)
                     /gene="lacD"
                     /locus_tag="ACHGNW_002658"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN6926913.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSI"
     gene            complement(957..1415)
                     /locus_tag="ACHGNW_002659"
     CDS             complement(957..1415)
                     /locus_tag="ACHGNW_002659"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN6926914.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1412..2875)
                     /locus_tag="ACHGNW_002660"
     CDS             complement(1412..2875)
                     /locus_tag="ACHGNW_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6926915.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2857..3789)
                     /gene="pfkB"
                     /locus_tag="ACHGNW_002661"
     CDS             complement(2857..3789)
                     /gene="pfkB"
                     /locus_tag="ACHGNW_002661"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN6926916.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3931..>4507
                     /locus_tag="ACHGNW_002662"
     CDS             3931..>4507
                     /locus_tag="ACHGNW_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6926917.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1349 a         1014 c          723 g         1421 t
ORIGIN      
        1 aatagaagtt gctgtttctt ctaatagtgt acttaattct ttaatatttt gttttccttc
       61 ttctaacatc caactactcg ctttttcttc gccttcggta acaaaaggtg ctactccctt
      121 cgcccatgtt gctcgcccac ataagacacc attgaatgtc gaacctgatt gttttgcaaa
      181 acgcaatgta tcttgaaata gtgcagcgct gactcccgca cttaaaaaaa taaacggttg
      241 ttcagtcact ttcgattgtt catagaaata cgcggatgcc tctgcttgac tataacaagt
      301 atcttgccca tatccttcca caaatctcat attcactggt acttctactt tcaatacatc
      361 cacttgataa cgttcttttg aaaattctcg catggcttca atcactttat gcggttttac
      421 ttttgcatag tcaagagaag acggatcgcc aatagttgca tcgtaagaaa ggagctctaa
      481 aaagaaaggt atgccttctg cttcgcattc tgaaccaatc cgttctataa ctatttgttt
      541 ttgcacatta atctctactg gttcatcaac gtcatagtac aataaaaatt tacaagcatc
      601 cgcacccact tcttttaacc ttttcactga ccaactattc agtaaatctg gtagtcggcc
      661 tggcattttc gcatcatagc cggttttttc ataagctagc aacaaaccac attctttctc
      721 tcgaagcgct gctgcaggca gaccatattc tggatcgagt aaaatagcgg aagcatacgg
      781 agtcaggtac tgagaaacca tctgtttaaa ttggatgata tcttctgatg tagcttcttt
      841 atattggttg atcattttct tcaaagcacc acgttggtcg atagctagcg cgctaataat
      901 tccagcttca tccgtcaatc gcttcaaacg ttcctgtttt tctatactca ttttcttcat
      961 atatcttcac ctatttcttt agttaatatt gtagctattt ctatttttga ttttgcagct
     1021 ctcaactgtt ctgtaacctc ttttcgcatc aatgatcgtg caacgagaga caataatttc
     1081 aaatgagtcg ttcctgcttc tgaatctggg ataaataacg caaaaataaa atcaactgac
     1141 tgtctatcta aactgttcca ttcaattcct tttttcagtg tgacgataat caaactaggt
     1201 tcattgattg tttgagactt cgcatgaggg atagcaaaac ctgacattat tcctgtagta
     1261 ccttcttttt ctctttccag aagtttttga tatacttctt tttcgtcatg agcgattcct
     1321 aaacagaatg tttgctttgc taaaaactta aatgcttctt cttgattatc taatggctgt
     1381 tgtaaaaaaa tgttctcttc atgaatgatt gtcatgataa acctcctgct cttccttctt
     1441 cgataactgt acgatttgtt gctttattat ccctcttctt gtctttatca gttactcttt
     1501 tcttttggat caaacctagt atcactccac caacgatcga accaataaga atcgctgtaa
     1561 cccattggat ttttcccgtc actacaggta acactaaaaa accgccatgt ggggctggta
     1621 cttggacacc aaacagatag gtcaagacag cagcaattga tgagcctaac atcatcgttg
     1681 gtaatactcg gatcgggtct ttcgcagcaa acggaatagc tccttccgta atatgagttg
     1741 atcctaaaat aaaattaacc aaacctgcat tacgatcatt agtatcaaaa tatttaccaa
     1801 acaataaagt agcaaaacca gtaatcaacg gtggtgcaat gcaagctgct gacgcaccag
     1861 ccataaaagt cgtgtttcct tgtgtcaaaa gagctgttcc tgttacatat gccgccttat
     1921 taaccggtcc tcccatatca aacgcacaca tacagccaac aatcacgcca agaacaagtg
     1981 gattcgaata ttcaaatcca gctaaaaagc tcatcattcc ttcatttata gccttcattg
     2041 gacctgccag caaagacatc acgatgccaa taaatgctac accaataact ggatataaga
     2101 cgattgcctt caagccatct agttgtttag gtaaaattat cagtagcttt tgcaaaaata
     2161 agataaaata acctgctaag aacccagcta aaatacctcc taaaaatcca gtaccgccat
     2221 tcatggctac gattccacca acaaatccaa cgactaaccc tggacgtttc gcgatccctt
     2281 ctgcaatata agctgctaaa actggaacca tcattcccat tgctgcatcg tcaactgctt
     2341 ttaccattgc cgctgtagca ttgtattgtg catgatctgg atcagaagaa tagattcccc
     2401 aaacggcaaa tgaaatagca atcaatactc cgccagctac cacgaaaggc aacatatgag
     2461 agacaccgtt catcaaattt ttatagatgc tacgaccaaa attagcacca cttatcgctt
     2521 ctggttctaa agtatcagca gcttcattat ctgctaagac agtacctttt cctgccaatg
     2581 cttcgtcaat cagccgttgt gcatccttga ttcctgtact gacagaaaca tcaatgactc
     2641 gttttcccac aaatctttct gaatggacat ctttgtctgc ggcaataatg acagcatctg
     2701 cctgtttgat ttcttcagcc gttaattcat tctcgatccc aatttgtcca tgggtctcca
     2761 ctttgatcgt gatgcctttt ttcctagctg cttgctctaa tgcctcttgc gccatgtagg
     2821 tgtgcgcaat tcctgtcgga caaccggttg ctgcaattag ttgatatttt cccatctttc
     2881 gaacctctct tccttgattt tgatttgttt acttaattcc tccacatcca aaaaatcagt
     2941 cagtccttta cgaaaagcag tagagctgcc tgctgctatg cttttacgta acgtttcatc
     3001 aagcgatcgt cgattcatgt atcctgctaa aaaagtgcct aacatggcat cacctgcaca
     3061 tgctgtattg acaacttttc cttttggcga gtttcctcga atcaccctgt ctttcgtaaa
     3121 aagaatcgct cctttctcac cgagggataa caaaacattt tctgcgcctg actctactag
     3181 tcgttgtcca taaacaaagt aatcttcatc tgtaaccatt tttcgtccga accaagaaga
     3241 aagttcttcc tcatttggtt tcagcaaaaa cggtcgatag ggtaaacaat ccatgacttc
     3301 ttgatcacta ctatcaataa tcaaacaaat ccgactgatt tctattaaaa cctttggaga
     3361 cagccctctt ggtaaactcc cagaaacaca aagataatcc cctgcttgta atgctcttat
     3421 ttgatttaaa aaattatgaa cagctgtctg cggtatttca ggtccttgat tcactaattt
     3481 gtattcttct cccgtttgat tgacttgtgt aaatacattg atacgcgtca taccaggcac
     3541 ttcaataaac tctgtttgga tttgtttctg ctgtaaatag tcttctatat actttcctgt
     3601 gaacccagca gaaaaaccta atgccgtact tgggaaatct agcatcttta atactagtga
     3661 cacattcacc ccttttccgt ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa
     3721 aggatgcatc tcatctgttt caataaatag atcaattgcc aaattcatcg tgcatgtgta
     3781 gatagacata accaacgacc tccttgattc tattatgaaa tacgcgaaat agaaaatgtt
     3841 ttcttaatcg tcccaacaca aagaaaacct ttacatttat aaactcgtcg tgcttttttt
     3901 aactaacttt tggtgaaaga aggaaagaat atgaatcaca aattaagtga aacagagcag
     3961 tacctctgga actttatcga acacctcatc ctagagatcc ctaactattc catcgtgaaa
     4021 ctaagtgaac aagcaaatgt ctcaacagcc accattgtgc ggacaatgaa aaagaaaggc
     4081 tatgaagggt ttacttcgtt taagcatcat ctaaaagaag gagaaaacaa gaacatcaat
     4141 ttttcatttt tagataaagt agacaaagga atccgccgag ccattctaaa aaatgaacaa
     4201 gaagtggtac gtacaatcaa tatgttggaa atcgggaaca ttgaagatgc aatacaaaga
     4261 atcaaatttg ctgaccgggt ttttgtcttt gcccgaggat tttcagaaat gattggccaa
     4321 gaaatgctgg taaaacttca attaacggga aaaaattgtc agatgcatac tgatccagaa
     4381 attatcaaaa gcatcagtcg gaaactaact aagaaagaca gcgttatttt tgtttctttg
     4441 aacggtgaga aaacaaataa aaacatcaaa aataagattt atgtctttat ttttcaattt
     4501 tttaaat
//
LOCUS       DAZBTH010000154         4452 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000154 DAZBTH010000000
VERSION     DAZBTH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.159"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(282..569)
                     /locus_tag="ACHGNW_002663"
     CDS             complement(282..569)
                     /locus_tag="ACHGNW_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6926918.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(589..813)
                     /locus_tag="ACHGNW_002664"
     CDS             complement(589..813)
                     /locus_tag="ACHGNW_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6926919.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1268..1438)
                     /locus_tag="ACHGNW_002665"
     CDS             complement(1268..1438)
                     /locus_tag="ACHGNW_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926920.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1453..1986)
                     /locus_tag="ACHGNW_002666"
     CDS             complement(1453..1986)
                     /locus_tag="ACHGNW_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6926921.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2007..2951)
                     /locus_tag="ACHGNW_002667"
     CDS             complement(2007..2951)
                     /locus_tag="ACHGNW_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN6926922.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3588..3674
                     /locus_tag="ACHGNW_002668"
     CDS             3588..3674
                     /locus_tag="ACHGNW_002668"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6926923.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3795..4445)
                     /locus_tag="ACHGNW_002669"
     CDS             complement(3795..4445)
                     /locus_tag="ACHGNW_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926924.1"
                     /translation="MLGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLSIVLSGL
                     FGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMNDIFVNQE
                     KRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNLFSDTIP
                     FSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQQQQN"
BASE COUNT         1249 a          816 c          681 g         1706 t
ORIGIN      
        1 gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa aaagactagc
       61 aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa ttgttgcaaa
      121 tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg ccatttacta
      181 ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac tgatgaatca
      241 gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca aataccaata
      301 gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa ttctattaaa
      361 gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact acttaatttc
      421 atactaactt caccaacact ttttccctca ctgatttctt tagaataaag aaagagcttt
      481 tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact aattgcattt
      541 aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact accttctagg
      601 tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt ctcctttagc
      661 ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc catagtaagt
      721 agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta atattcctag
      781 aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc gtttttttaa
      841 aattgcttgt tatattaaat aatcttagta attttattat ataacatttt attatataac
      901 aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa aattataaga
      961 tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg cctgtaccaa
     1021 gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct ctttttaagg
     1081 cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg tcatttttta
     1141 aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata ttcatttatt
     1201 attcttttct aaggttaata ataggataat ctctttcaga aagactgctc gtacgagcag
     1261 tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg ttgtagtaat
     1321 taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat aataacattg
     1381 ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt tttgtcataa
     1441 gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg tttgttcgtc
     1501 cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact cttccgtata
     1561 agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca gggtgagttg
     1621 ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga gggcaagttg
     1681 ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg caaccacgtt
     1741 tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca acttatctag
     1801 caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca atacaacctt
     1861 ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat gtttcgatat
     1921 tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg tttcttgtgg
     1981 ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc agataataaa
     2041 ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc ttttttctct
     2101 ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg tttgattttt
     2161 tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa agccaatgct
     2221 ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca
     2281 ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc gtatttcttt
     2341 ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt tataactcgt
     2401 ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag aaagtcttct
     2461 ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa catagtcttt
     2521 gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa
     2581 ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg attttctacg
     2641 tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag cccagactca
     2701 cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa atcttctaca
     2761 aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa
     2821 cgaactgtct tatccccttt ttcacgcatt ctagacacga tggaaaaaaa caaattcatt
     2881 tcaaccggtg taaattttct taaaggaatt gtatttaatt catggtggta ttttacaatc
     2941 tcattagcca tataatcacc tcaatccaat tatgaatgac attttttaaa tgtcaagaca
     3001 ttcatatttg acgttcaaac agacaaaatg tccttcataa acagacaaaa tgtccttcat
     3061 aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataacatt ctgtatccat
     3121 tggtagagta aggacacagc ttacggtaaa gaggtttaaa aagaggttta aaaagagata
     3181 taaaaagagg ggggctaatt ttcccgccta aaaaccactt cttttttctt taaacaggtt
     3241 cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt
     3301 gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat
     3361 gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt aagacagtct
     3421 tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc aagccctaac
     3481 aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta agatataata
     3541 acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg tttgaattgt
     3601 tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg ttgaataacc
     3661 gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc cagagaatcc
     3721 gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta gtttaacatg
     3781 attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac actttttaga
     3841 taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg ctgaagcagt
     3901 gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg ttcactagtt
     3961 gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg cgtatcttta
     4021 aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt ttgctgtttt
     4081 tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt caaacgtttt
     4141 tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc ttccattttt
     4201 tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt ttgagtgata
     4261 tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc gaataaacca
     4321 cttaaaacga ttgataatgt aaatataacc cctggcaaca aaaacctctt aaatcgaatt
     4381 ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca aataattcct
     4441 aaaatactgg ca
//
LOCUS       DAZBTH010000155         4425 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000155 DAZBTH010000000
VERSION     DAZBTH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.160"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHGNW_002670"
     CDS             690..1658
                     /locus_tag="ACHGNW_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6926925.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACHGNW_002671"
     CDS             1726..2484
                     /locus_tag="ACHGNW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFN6926926.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHGNW_002672"
     CDS             2552..3262
                     /locus_tag="ACHGNW_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6926927.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHGNW_002673"
     CDS             3298..>4425
                     /locus_tag="ACHGNW_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN6926928.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZBTH010000156         4368 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000156 DAZBTH010000000
VERSION     DAZBTH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.161"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHGNW_002674"
     CDS             complement(37..1257)
                     /locus_tag="ACHGNW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6926929.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHGNW_002675"
     CDS             complement(1277..1888)
                     /locus_tag="ACHGNW_002675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN6926930.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHGNW_002676"
     CDS             complement(1931..2863)
                     /locus_tag="ACHGNW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN6926931.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHGNW_002677"
     CDS             complement(3284..4144)
                     /locus_tag="ACHGNW_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6926932.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4276..>4368)
                     /locus_tag="ACHGNW_002678"
     CDS             complement(4276..>4368)
                     /locus_tag="ACHGNW_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN6926933.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIQA"
BASE COUNT         1242 a          881 c          716 g         1529 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag cttggattcc caataatacc ttgatttcag tacagaccga
     4321 aaacccgaag agagtgcctt cttttcgggt tttcttatat aatcctcg
//
LOCUS       DAZBTH010000157         4348 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000157 DAZBTH010000000
VERSION     DAZBTH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.162"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHGNW_002679"
     CDS             complement(387..1853)
                     /locus_tag="ACHGNW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6926934.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHGNW_002680"
     CDS             complement(2144..4228)
                     /locus_tag="ACHGNW_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6926935.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAZBTH010000158         4326 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000158 DAZBTH010000000
VERSION     DAZBTH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.163"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACHGNW_002681"
     CDS             complement(<1..283)
                     /locus_tag="ACHGNW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN6926936.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACHGNW_002682"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACHGNW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACHGNW_002683"
     CDS             complement(2361..2723)
                     /locus_tag="ACHGNW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926937.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACHGNW_002684"
     CDS             complement(2727..3035)
                     /locus_tag="ACHGNW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926938.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACHGNW_002685"
     CDS             complement(3044..3463)
                     /locus_tag="ACHGNW_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926939.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACHGNW_002686"
     CDS             complement(3456..3689)
                     /locus_tag="ACHGNW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926940.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="ACHGNW_002687"
     CDS             complement(3686..>4326)
                     /locus_tag="ACHGNW_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6926941.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DAZBTH010000159         4292 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000159 DAZBTH010000000
VERSION     DAZBTH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.164"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..1461
                     /locus_tag="ACHGNW_002688"
     CDS             160..1461
                     /locus_tag="ACHGNW_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6926942.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1488..1748
                     /locus_tag="ACHGNW_002689"
     CDS             1488..1748
                     /locus_tag="ACHGNW_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6926943.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2747..3982)
                     /locus_tag="ACHGNW_002690"
     CDS             complement(2747..3982)
                     /locus_tag="ACHGNW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6926944.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1250 a          679 c          882 g         1481 t
ORIGIN      
        1 atgatttaga aaacttattt tcaggattct taaaaataaa tttgattatt taagagaata
       61 tattgaaata aaatttctta attatataat tagttatgaa cattctttgc gttgtcatgc
      121 aatgaatggt tcatatttta ttgaaaagga gtggttttag tggaaaagct gatgaaatgg
      181 gtcgaagaga aacttgtccc accaatggca aaaattggta cacagcgtca tttattagca
      241 attcgtaatg gggttgtttc aacattgtca ttgattttga ttgggacatt ttttatggtc
      301 tttatcaatt taccgtttcc tggatggaat gaatttattg ccccttattc ggcaacgatt
      361 gttttaccct ttaggatgac gatgggattg atggctattt atgcaacttt tgttatgggt
      421 tcggatctag ctaaatcata tggattagat agtgtgacag gtggtatatt atctttagga
      481 acgttcttta tgttacaagt tcctgtaaac gttttaactc ctgaagaggc acctcttggc
      541 tgggtcttac ctatgagttc tttgggagca tctgggatgt ttgcggggat cttatctatg
      601 atttttgcag tggaagtata tcgtttcttt aaaaatcgaa atattactat caaaatgcca
      661 gaacaagtgc cacctgctgt tgcccgtagt tttgaggcat tgattccagg tgcagtcatt
      721 ttaacaatta cttggctgat tcgaagcgtc gcgggctttg atgtaaacgc tgccttatta
      781 tcgttattcg agcctttaac caatatttta gggaataatt tgcttggtgt tttactacca
      841 atgtttttga tccatttgtt gtggtcattt ggtatccatg gcatgagtat tgtaggggct
      901 gttgttcgtc caatgtggct gatcatgttg gatgagaatg caaaggcatt ggcagatggt
      961 acagctgcca cccaattacc ctatatcgct cctgaacagt tttatcaatg gacggtaaca
     1021 attggtggtg caggagcaac gatcgtggta tctgttttgt ttatattctt ctgtaaatca
     1081 aaattcttga aagaggttgg tcgtttttct attattcctg gtatcttcaa tatcaatgag
     1141 cccataattt ttggagctcc tatgatgttg aatccttaca tgttcatccc gttcaactta
     1201 gttcctttag ttttgactat tatcagttat tttgcagtca aattagaaat ggtcaataga
     1261 tttactgttt tgccaacatg gacattacca gctccaatcg gcggttatct ttcagcagga
     1321 aatgactggc gtgttgttat cttgatcatg atcaacacat tgatagcatt tattatctat
     1381 tatccatttg taaaagctta tgataaaaag atgctgtcag atgaacaaag tcgtggagaa
     1441 aacgaagtcc agactgtcta agtttggttt ttagaaggtg ggaaacaatg tattggctga
     1501 ttttattttt tgtttttatc tttttattaa cggctagtca ccttatattg aatatgctag
     1561 ctacatatca tattcaaatc aatcgatgga tttgggcact ggcatcattt ttgatcgtga
     1621 ttcttccaaa gatcattgta ccgcatatga atgttttgtt ttcatggggg acatatgtac
     1681 tttgtggaat ttttgctatc aattttatga tcgagcagca tagatggttt gtcacatcga
     1741 agctttaaat gttctaaaag tggttgtaac agaagtggtt agcttcaaga aataagaaga
     1801 aatttacgaa aattgcttct taaatttttg tgaaatgttg gcttattttt cgtttggttg
     1861 aacttattac cgcagatgca agcttttgac caccgtttat tcggaatatg ggactgtgac
     1921 aaggcttttg tcacagccct tttttacata aagattatta ttgttttaac tgttttggca
     1981 gaaagaagga ggaaaaaatg gaaattttat cagcaatgga tttagaatta gtagatctac
     2041 taccaaagtg atcaagtcaa atatagattt gggagcttac aaccatatag taggggctgc
     2101 agtatggtgt atgaatggta atatgtatga tgtgagtgtt tattcaaatc atgaaacgtg
     2161 tatagaaata attgtgctgg gaatagcaat cagtcatggt gaacgtgact tttagtgtat
     2221 cgttgctgac gacatagaac attctgacat gatctactca atatgtgggg attgctggca
     2281 gtttatatag gagtatgcat cggattgcga gatcattgtc tcaacagaag agggaggttg
     2341 attatcaaag ataaagtggt aaatcctaca tttgatttag taattgttga ggggactagc
     2401 ggcacaaatc tctacaaagc gatgcttcta gatctatatg acacatttta agaaaatgta
     2461 tttgcctaat tattatggtc tttcttttga agaaacgttg agatgatcca accagagaaa
     2521 ggtgtcaaag aatttgacta agaataaatg gcagacgcta cctatgctca tttggatagc
     2581 gtcagatctt ttattttaag gatgattttg tgttttttag acaccgtaat ttaagtaact
     2641 ctaacttttc aatccactat ttactttttt tgtattttca ctgtctgggt ctcgaatagc
     2701 gaaattactg atgagtctct attaaaaaag ctttttctac ttgaaactat attgggtggt
     2761 tgctcaggaa ggtttcgatt tcttgaattg cttgttgtgg accatttatt ggaaaaacgg
     2821 cttgcagcgt gcgtgcctgc tgacgaatct tgggtgaaga aacttcgcgc agagcaatct
     2881 gcagttttct ttcagtcact ggttttgtga gggcgattgc gtttccgtat tttacacaat
     2941 agtcaatatt acagctttgc tcaaattgca tccccacacc gataaacggt ttgccggatt
     3001 cacaggcagt ttggacggtc ccttccccgc cgtggataaa agaaaaatca atgaattcat
     3061 gcaaaagatg actagacaga tattctgtta ttaaaagatc ttctgagtca gattgaatgt
     3121 catcaagcag atagtcaata ggtgcaataa ccgctagatc cggttgtttt tgtaagaaga
     3181 acaaaatttt gcgtaacata cgcggattgc ctgaactacc cattgcgaaa tagataatac
     3241 gttttctttc ttgttgcttt cgctgtataa aagttagcac ttcttcaggc aaggaagtac
     3301 tgagcttagc aaaaattgga ccaacgattc gataattttc cggcagctgt tcttcttcca
     3361 aaaatagttt aggcgttgtg atgagattta gatcagcatc catcatttgt aaacttgttt
     3421 ttggtaaagg aagcatttct ttttgtgcag cgtgtttgaa ggcattgggg atccatcggg
     3481 tatttactat acgctttttt gtccagtgcc aaaggggatt gcttaaaggg ccgaagaaac
     3541 gtaactcgtg gggcatcagc gcatgctctg attgaaaaaa aggttcactc aaagcatagg
     3601 gtttaacata tactaaagga atctttttat tgcgggccga taaaaaaatt gtcacattcg
     3661 aacctgtcac aatcagtcga ggctcaaact tagcaatcag actctgttca ctagtgatac
     3721 gagaggcaac catttctttg gtaaaagggt ttttaagtgt acgcaactgg tcaaatttta
     3781 tgatttgttc ttcttgtttt ttcgtaagga tcggcgctag taaatgatac tcaaagccac
     3841 tttctgtgat caaaggagca aaagttttag aaaagccaaa gaatacacag taataacggt
     3901 ctttcatagc tttggcaacc tcaatcattc gtgttgtctc agctaaattg aatactgcag
     3961 gagcaaaaag aattgtcttc atgaaatcct ccttattgga atgagtctat cccttcgttt
     4021 cttactagta tactaaaaaa cacagcgttc gagtagccta tgtatatttt gaatattaaa
     4081 ctgtctctcg ctgttttcac aacttctaaa tttaaatttt gtaatcaagt tgaaactcca
     4141 attttcaaaa aaacacgcaa caattttttg atcgatttat atgttgtgcc agataaatac
     4201 gagaagttgt gtcattaaaa ataaacatga gaacataaaa aaataaaata attatattaa
     4261 ttgataggtg tgctgtcgtc attatacaca cg
//
LOCUS       DAZBTH010000160         4153 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000160 DAZBTH010000000
VERSION     DAZBTH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.165"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHGNW_002691"
     CDS             <1..106
                     /locus_tag="ACHGNW_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6926945.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACHGNW_002692"
     CDS             complement(359..508)
                     /locus_tag="ACHGNW_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926946.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACHGNW_002693"
     CDS             970..1182
                     /locus_tag="ACHGNW_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926947.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACHGNW_002694"
     CDS             complement(1460..3259)
                     /locus_tag="ACHGNW_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN6926948.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACHGNW_002695"
     CDS             complement(3383..3979)
                     /locus_tag="ACHGNW_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6926949.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DAZBTH010000161         4089 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000161 DAZBTH010000000
VERSION     DAZBTH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.166"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            405..926
                     /locus_tag="ACHGNW_002696"
     CDS             405..926
                     /locus_tag="ACHGNW_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926950.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
     gene            1042..1362
                     /locus_tag="ACHGNW_002697"
                     /pseudo
     CDS             1042..1362
                     /locus_tag="ACHGNW_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1540..1899
                     /locus_tag="ACHGNW_002698"
                     /pseudo
     CDS             1540..1899
                     /locus_tag="ACHGNW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1963..>4089
                     /locus_tag="ACHGNW_002699"
     CDS             1963..>4089
                     /locus_tag="ACHGNW_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6926951.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENY"
BASE COUNT         1500 a          558 c          754 g         1277 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactat
//
LOCUS       DAZBTH010000162         4074 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000162 DAZBTH010000000
VERSION     DAZBTH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.167"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1688)
                     /locus_tag="ACHGNW_002700"
     CDS             complement(183..1688)
                     /locus_tag="ACHGNW_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6926952.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1685..2371)
                     /locus_tag="ACHGNW_002701"
     CDS             complement(1685..2371)
                     /locus_tag="ACHGNW_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6926953.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2564..3985)
                     /gene="gndA"
                     /locus_tag="ACHGNW_002702"
     CDS             complement(2564..3985)
                     /gene="gndA"
                     /locus_tag="ACHGNW_002702"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN6926954.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1122 a          859 c          694 g         1399 t
ORIGIN      
        1 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
       61 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      121 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      181 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      241 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      301 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      361 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      421 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      481 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      541 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      601 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
      661 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
      721 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
      781 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
      841 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
      901 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
      961 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1021 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1081 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1141 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1201 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1261 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1321 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1381 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1441 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1501 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1561 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1621 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     1681 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     1741 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     1801 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     1861 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     1921 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     1981 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2041 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2101 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2161 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2221 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2281 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2341 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2401 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2461 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2521 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2581 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     2641 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     2701 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     2761 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     2821 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     2881 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     2941 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3001 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3061 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3121 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3181 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3241 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3301 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3361 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3421 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3481 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3541 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3601 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     3661 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     3721 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     3781 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     3841 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     3901 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     3961 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4021 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZBTH010000163         3944 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000163 DAZBTH010000000
VERSION     DAZBTH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.168"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1211)
                     /locus_tag="ACHGNW_002703"
     CDS             complement(<1..1211)
                     /locus_tag="ACHGNW_002703"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6926955.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..2796)
                     /locus_tag="ACHGNW_002704"
     CDS             complement(1201..2796)
                     /locus_tag="ACHGNW_002704"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN6926956.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            2921..3706
                     /locus_tag="ACHGNW_002705"
     CDS             2921..3706
                     /locus_tag="ACHGNW_002705"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFN6926957.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            3699..3941
                     /locus_tag="ACHGNW_002706"
     CDS             3699..3941
                     /locus_tag="ACHGNW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926958.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1233 a          762 c          640 g         1309 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
     2581 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     2641 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     2701 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     2761 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
     2821 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
     2881 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
     2941 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
     3001 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
     3061 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
     3121 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
     3181 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
     3241 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
     3301 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
     3361 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
     3421 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
     3481 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     3541 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     3601 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     3661 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     3721 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     3781 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     3841 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     3901 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DAZBTH010000164         3941 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000164 DAZBTH010000000
VERSION     DAZBTH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.169"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1466
                     /locus_tag="ACHGNW_002707"
     CDS             <1..1466
                     /locus_tag="ACHGNW_002707"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN6926959.1"
                     /translation="QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDIL
                     GYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTD
                     EQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSD
                     MMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAK
                     MNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRF
                     NRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSI
                     YAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKI
                     LETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQ
                     EQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1456..2673
                     /locus_tag="ACHGNW_002708"
     CDS             1456..2673
                     /locus_tag="ACHGNW_002708"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6926960.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2686..>3941
                     /locus_tag="ACHGNW_002709"
     CDS             2686..>3941
                     /locus_tag="ACHGNW_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6926961.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1364 a          674 c          821 g         1082 t
ORIGIN      
        1 aacaagtggt tcttttagca gatgaatcct tagaggaata tgacacagta agcaaacaaa
       61 caatgttgta tcgtgaatta ctttcagatg aagaatcaaa agaagatctg attgccacaa
      121 tcgttgatat tttgggatat gcaattgcac cagagtatct tttcaacgtc ttagcggatc
      181 aagcaaaaca agcaacgttc caattgaacg atctaaacaa agcctttgtg caattggcat
      241 ctacctacaa tcaatttaat gggttatttg atgatgtgga tctccaatca aaaaaactag
      301 gaacagatga gcaacaacga aatgtgacga ttaccgaagt catcaagaaa ttgaatgatg
      361 tagatgtttt agaacacgat ggggacgtga tcggggatgc gtatgagttt ctgatcagtc
      421 aatttgcttc agaagcaggg aagaaagccg gagaatttta cacacctcat atggtttctg
      481 atatgatggc gcaaatcgta acgctcgatc aaaaggaacg acgattcttt agtgtttttg
      541 atccaacgat gggttcaggt tcattgatgt taaacgtacg taactactta acacacccag
      601 ataacgttaa atatcatggg caagaactaa atacaacgac atacaatcta gcaaaaatga
      661 acttgatttt acatggtgtc gacgccgaag aaatgaatct ccgcaatggg gatacgttga
      721 ataaggactg gccaacagat gaaccttaca catttgatgc agtagtaatg aacccacctt
      781 attcggctaa ttggtcagca gatacgactt tcttagatga ttctcggttt aatcgttatg
      841 ggaaattagc gccaaaatcc aaagcagatt ttgcgtttct tcttcatggg ttctatcatt
      901 tgaaggaaac aggtaccatg gccatcgtct taccacacgg agttttattc cgtggcgctg
      961 cagaaggggt catccgtcaa aaattactag aagatggcag tatttatgct gtgatcggta
     1021 tgcctgcgaa cttgttcttt gggacatcga ttccaacgac ggtgattgtg ttgaagaaga
     1081 atcgtcaaac tcgagatgta ttattcattg atgctagtcg tgaatttgtt aaagggaaaa
     1141 atcaaaataa actatcggaa gaaaacattc aaaaaatcct cgaaacctac gctgaaagaa
     1201 aagatgtcga gaaatacgct catttggcaa cctttgatga aatcaaagaa aatgactata
     1261 acttaaatat cccacgctat gtagatactt tcgaagaaga agaaccaatt gatatggttc
     1321 atgtcggaaa tgacatcaag aagatacgac aagaacagca agtgctagaa aaaaaacttt
     1381 tagaagctat ctcttccctg caaacaacac ctgaaaatga agcgtggtta caaggtgcgc
     1441 tggaggtgtt caaacatgag caatgataca caaccagaaa tccgtttccc aggatttaca
     1501 gatgattggg aacagaggaa gttgggggac ttcgttgtag attatgttga aaagacaagt
     1561 gtacaaaatc aatttcctat gctaacttca tctcaacaaa aagggatagt tttgcaagaa
     1621 gactattttg caaatcggca agtaactaca gaaaataata ttggttactt tgtactacct
     1681 agaggatatt tcacgttccg tagtcgaagt gataatgatg tgtttgtgtt taaccgtaat
     1741 gacattattg atagagggat tataagctat ttttatcctg tatttacttt aaaatctgct
     1801 gattccgact tcttcttaag acgtattaac aatggaatcc aacgacaatt atctattcaa
     1861 gcagagggaa caggtcaaca tgttctgtct ctaaaaaagt ttaaaaatat agtagcaatg
     1921 tttcctagtg aggaagaaca acaaaaaatc ggcaccttct tcaaacaact tgacgacacg
     1981 atcgctcttc atcagcgtaa gttagatttg ctgaaggaaa cgaaaaaagg attcttacaa
     2041 aaaatgttcc ctgaaaacgg agcaaaagtg ccagaaatcc ggtttcctgg ttttacggaa
     2101 gattgggaag agcgtaagtt gggagaatta ggtaaaactc aatcgggaat tggatttcct
     2161 gatgccgagc aaggtggttc tgaaggtact ccatttttta aagtttccga catgaataac
     2221 attggtaatg agtatgaaat gagaaatgca aatcattatg tatctaatga gcaaattgag
     2281 cgtaaaaaat ggaagccaat aaaagatgtg ccagcgatta tatttgctaa agttggcgca
     2341 gcaataatgc ttaatagaaa acgtttggtc acaagccctt tcttaataga taacaatacc
     2401 atggcttatt tatttaataa tacttgggat atctattttg gtaaaatatt gtttgaaact
     2461 ataaatttgc caagatactc ccaagtaggg gcattgcctt cttataacag ttctgatatt
     2521 gagaatatca gtgttaaggt tcctgtaaag gatgaacaac aaaaaatcgg tactttcttc
     2581 aaacagctag atgatacgat cgctcttcat caacgtaagt tagatttact aaaagaaacg
     2641 aaaaaaagct tcttacaaaa gatgtttgtc taggagggaa gaagaatgaa aattagtcac
     2701 cgtgaggaag cagaagtcga agaacagttg attcgtgtac ttggagaagg gcataatcag
     2761 tggacgtatc gtccggatct aaaatcagaa gaagaccttt gggtcaattt acgccaaaaa
     2821 attatctcaa ataatcaagc agaactgaat gattttcctt tgaccgataa agaatttgaa
     2881 acgatcaaaa cggaattact gctgcgaacg aaaaccccat ttgatgctgc gaaatggtta
     2941 aaaggtgaaa atgggatggc tcggatcacg attgaacggg aagattcgca attgggttct
     3001 gtctcactta tcctttattc caatcaagac attggtggag gaatctctac ttatgaagtg
     3061 gtccatcaaa ttgcaaaacg tggaagtaac atagaagcac gggatcgacg ttttgacgtc
     3121 accttattaa tcaatggatt accgattgtc caaatcgagc tgaaacaagt aactgcaaaa
     3181 gatggtgttt atcaggcatt taaccaaatc aaaaaatatg cagaagaagg tatgtttcgg
     3241 aacaatatct tttcgacctt acagttgttt gtggtttcca atgaacaaac cactcggtat
     3301 tttgcaaatg ccttaccgaa agacttgcac cctaagtttt tatttagttg gcgaacgaaa
     3361 gacaatgaaa aagtagaaaa cttatatgag ttttgtaaac aagtgttaaa tatcccggat
     3421 gctcatcgtt tgattgcgga ttatacaatc gtcagtgaag atcaagacaa taaaaccctc
     3481 atggtgttac atccttatca agtacatgcg attcaagcct tgtttattgc cgcaaataaa
     3541 catcaatcag ggtatgtgtg gcatgcgact ggttcaggaa agacgttgac tagttttgta
     3601 tctactaagc ttttagcacg aaaatcgggg atcgatcgaa cgatcatgtt agtggaccgc
     3661 aaagatttag ataatcaaac caccacagag tttacaaaat ttgcttcgga atttaatact
     3721 gggatttctt ccgggaacgc caaagcgaat agcttgatcg ttggaacggg aagtgccaaa
     3781 gaattaagtg aaaccctgtt ggcagatgcc aatgcaaatg ttgtaatcat tacaacccgt
     3841 caaaaactag atgcggcgtt gaaatatgca aagaaacaag aagagaaaaa aggaaccaat
     3901 cgcttccaaa aattaatggg ccaacatatc gtgtttgtag t
//
LOCUS       DAZBTH010000165         3876 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000165 DAZBTH010000000
VERSION     DAZBTH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.170"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACHGNW_002710"
     CDS             158..2257
                     /locus_tag="ACHGNW_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN6926962.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACHGNW_002711"
     CDS             2453..3316
                     /locus_tag="ACHGNW_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN6926963.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACHGNW_002712"
     CDS             3421..>3876
                     /locus_tag="ACHGNW_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6926964.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZBTH010000166         3806 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000166 DAZBTH010000000
VERSION     DAZBTH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.171"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1003)
                     /locus_tag="ACHGNW_002713"
     CDS             complement(<1..1003)
                     /locus_tag="ACHGNW_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFN6926965.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     "
     gene            complement(1007..1417)
                     /locus_tag="ACHGNW_002714"
     CDS             complement(1007..1417)
                     /locus_tag="ACHGNW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6926966.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1435..1926)
                     /locus_tag="ACHGNW_002715"
     CDS             complement(1435..1926)
                     /locus_tag="ACHGNW_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6926967.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1936..2727)
                     /locus_tag="ACHGNW_002716"
     CDS             complement(1936..2727)
                     /locus_tag="ACHGNW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6926968.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2720..3493)
                     /locus_tag="ACHGNW_002717"
     CDS             complement(2720..3493)
                     /locus_tag="ACHGNW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6926969.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3649..>3806)
                     /locus_tag="ACHGNW_002718"
     CDS             complement(3649..>3806)
                     /locus_tag="ACHGNW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFN6926970.1"
                     /translation="TNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYT
                     IFEQTIF"
BASE COUNT         1183 a          794 c          514 g         1315 t
ORIGIN      
        1 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
       61 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
      121 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
      181 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
      241 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
      301 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
      361 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
      421 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
      481 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
      541 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
      601 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
      661 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
      721 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
      781 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
      841 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
      901 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
      961 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     1021 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     1081 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     1141 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     1201 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     1261 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     1321 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     1381 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     1441 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     1501 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     1561 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     1621 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     1681 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     1741 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     1801 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     1861 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     1921 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     1981 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     2041 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     2101 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     2161 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     2221 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     2281 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     2341 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     2401 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     2461 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     2521 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     2581 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     2641 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     2701 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     2761 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     2821 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     2881 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     2941 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     3001 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     3061 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     3121 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     3181 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     3241 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     3301 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     3361 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     3421 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     3481 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     3541 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     3601 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     3661 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     3721 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     3781 tccctctttc aaatgttcat ttgtta
//
LOCUS       DAZBTH010000167         3713 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000167 DAZBTH010000000
VERSION     DAZBTH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.172"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHGNW_002719"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHGNW_002719"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN6926971.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHGNW_002720"
     CDS             complement(2261..2512)
                     /locus_tag="ACHGNW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN6926972.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHGNW_002721"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHGNW_002721"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN6926973.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHGNW_002722"
     CDS             3462..3680
                     /locus_tag="ACHGNW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926974.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAZBTH010000168         3698 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000168 DAZBTH010000000
VERSION     DAZBTH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.173"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..266
                     /locus_tag="ACHGNW_002723"
     CDS             <1..266
                     /locus_tag="ACHGNW_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926975.1"
                     /translation="VAKTCWTMLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQ
                     FNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(272..454)
                     /locus_tag="ACHGNW_002724"
     CDS             complement(272..454)
                     /locus_tag="ACHGNW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926976.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(454..753)
                     /locus_tag="ACHGNW_002725"
     CDS             complement(454..753)
                     /locus_tag="ACHGNW_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926977.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(858..1061)
                     /locus_tag="ACHGNW_002726"
     CDS             complement(858..1061)
                     /locus_tag="ACHGNW_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6926978.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1076..1822)
                     /locus_tag="ACHGNW_002727"
     CDS             complement(1076..1822)
                     /locus_tag="ACHGNW_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN6926979.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            1900..2208
                     /locus_tag="ACHGNW_002728"
     CDS             1900..2208
                     /locus_tag="ACHGNW_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926980.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2179..2436)
                     /locus_tag="ACHGNW_002729"
     CDS             complement(2179..2436)
                     /locus_tag="ACHGNW_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFN6926981.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(2734..2874)
                     /locus_tag="ACHGNW_002730"
     CDS             complement(2734..2874)
                     /locus_tag="ACHGNW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926982.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            3174..3494
                     /locus_tag="ACHGNW_002731"
     CDS             3174..3494
                     /locus_tag="ACHGNW_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6926983.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3512..>3698
                     /locus_tag="ACHGNW_002732"
     CDS             3512..>3698
                     /locus_tag="ACHGNW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6926984.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHF"
BASE COUNT         1075 a          664 c          630 g         1329 t
ORIGIN      
        1 ttgttgctaa aacatgttgg acaatgttaa ataaaaacca acgcgatgtt gaaaatattg
       61 ctatattttt aattaagatt acttcctcaa tatcaattat cattccgatg actgtaactt
      121 tttcaaaaga acagtttaat gtagcggcta cttatctttc atttatagcg ctattttctt
      181 ttgttcttcc taaaagaaat aagagcaact ttgacaacaa ggagctggat gaagtacgca
      241 atagcagccc aaaggaacaa aaataaaaaa atcatatttt agtcagtccc tcccgactgg
      301 cttttttcgc tctgtgctca gcttcatcaa gccccataaa aatccaaacc atgtaaacga
      361 ttgtccctat caacgcttgt ctgcttcccc agaggcctaa agcgtagatg attagcggtg
      421 cgctgaatac tagtgctctg ttgaatttgc ccattaactt gcctccttca aaacatctga
      481 aggcgataaa ctttttacac gatatttgtt tgcgtctttc cactttaaaa accaaataaa
      541 agtatggtaa tgaataaatg tggttgagtg acctggacga agtattcctt ctgcaaattc
      601 aggaattgtt tccatttcct tgcagtattc aactaaagta gtttttgaca tgccgtggaa
      661 cttctccaag attaaacttt gtcggtacca gtcgtctggt tttactgcct tctcagcagc
      721 ttctatcagc tctgaaagtg ttggtttttt cacttattat ccctcctaaa aattatcgtt
      781 ccatactcga tgtttaacat ttcttgaagc tctgattttg taagaatgat tttatcttcc
      841 aacgacttta cctccctcta caattcgtac atagtgataa tcgaatctat aattctgttt
      901 gcttctgcag aagtcttttt accgtttaaa attaaagata agtagctttt gctaattcca
      961 aatctttcag caagcatggt gtaagttaag aactttgaac tttcgacata ttctttgatt
     1021 ttttctctat ctcgttgagt gatttctgca atgtcagtca tactaaaact cctttctaac
     1081 caatttcctc taaatccatt tgagggtaat agccctctgc gattaataag ttgtaaataa
     1141 acactcgccc tttctgtgtc catttggtat tcattacaac tttagtgccg ccatcggctt
     1201 tcggtatctc acttgtgtgc gattttgtgt atccttggtt catatgtttt cggtaaagta
     1261 tccattgtcc gctaacttta tgctgaacac ctaactcgtt tagcaatttg tttagagcaa
     1321 ttgccgacat tccgtaatca gctgcaattt gagaagtagc taccgtatct gtcgatgaaa
     1381 gaatcgtatc taagtagcta atctttggtt cgtactcggc aatttgttgt tcgagtaatt
     1441 ggttcttttc ttctaaatcc gctgctaaac gcaatgcttg tgcaaaactt tgaggtacgt
     1501 ttgaatagct acctgttttt ctaattgttg gaaggacttc actcgttacc caacgtttaa
     1561 attttttggc agagggaagt tttgatttta agattaaact gtaaaggcct gactcgttaa
     1621 tcaaaacaac tgctgttcca ttgacggtga acgaatcgtt ctgcgtctta tcctccaagt
     1681 ccacatgatc tcgaatagct ttttgcggat tcgaatatcc tagaacgtct gctacatctt
     1741 ttcctacaaa atatggttca tcatttacta aaatagttcg aacttcgttt tgctcgaaat
     1801 taaaaatttg tggtgtattc atatttctca ttcctttctt tggtataatt tccttatcag
     1861 tgtgacaggc tgaaataatt gttaaggagg tgaaaattta tgaaaccaga atttgtaatt
     1921 actatgcttg atggagaaaa aatcaaaata tttgccgata ctttggtcgt tggatacgat
     1981 gatgcgtcaa atatcgaaga aaaagatgga atgttctatt cttctcaaat ttacgtgaat
     2041 accttgcaag gtgatattgg ggtatctact cttgcaacat caagaccgct tgtaggtctt
     2101 atcggctttt tgtcaaaagc aacattcttt tctctcggcg atgatataga ttcggatgaa
     2161 actgtcatct ataaaacatc agctgttaaa agtatcggtt tcaaatagta attcttgctc
     2221 gttgatagcg gaagccaact tttcttccaa cacatcggcg agctttcttt ttgttggatt
     2281 catttcaaag ttgatagtct tgagaagcat ttttgcatcc ttaacttttt gtccttgcaa
     2341 ttccttgatg attttatcgg cttggctatc aattccatgt gttagatcat ctatcatttt
     2401 ttgtaccttg attgtttggc tcatttcctt gttcattgtt tagcctcctt taataaatga
     2461 ttttttgtat tttttaattt ccataaattc ctcaaattgc tctttggtat tattaccacg
     2521 accatacata ttgtgaaatt ctttatggca actgacacaa agtgtaacgc cgtttgaaat
     2581 cgctgcggat ttagttgttg catggctaaa tcacgaatca caagtatcta ctaaaggcga
     2641 atcaatttcg ccaaaagaaa ttgttcaagc ttatttagat attcattatg ctgttatcta
     2701 tggtcagctt ccagaagacc gtaaaaacga tgactaaacg tgagctaaaa tctctgctat
     2761 ggctgcaacc atggcagagt cttctttatc taccgcgtgt tccatcgcca cttttgcttg
     2821 tttcagtatg tctaatttca aatcttcgat ttttgccgag actgtttttt ccatattctt
     2881 ctctcctttc ttttatattc gtaaacaaat ttaacaactt tttaaaaaac tgcgttgact
     2941 ttattagatt aataatctat actaaatgca tagttaaata agacatataa actttgattt
     3001 aaaaagcttt cttggcggtt ggcatttatt aatcaatagt attttttgtt gtctttttag
     3061 ttgttaaact tgtttacaaa acatagtata gattaataat gtatataagt caactatttt
     3121 tacattattt atctaaactt tttttgtaag cattcagaaa ggttgattta tcaatgaata
     3181 cttacgaaat aataaaggag ttagcaaaag aaaaaggatt atccattaga cagctcgaaa
     3241 tgaatttcgg gtattcgaat ggatatttag gaagttggaa aagacaaact cctaattcta
     3301 ttgagttagc tcggttagct gattactttg gagtctctgt agactacctt ctaggtagag
     3361 aagaaagaga aacccctaaa catgtggatt tatcagaaaa cgatactgtt ttttcttttg
     3421 atggaaaaga aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag
     3481 aggaaaatga atagttggag tgatgggttg tatgtatttg aaattgaaag aaatgttgag
     3541 cgaatacaat ttgaaagtag tttacatgga aatgaaagag cctggcttct attatccgaa
     3601 gcctagaatc atttttttga atgaaaatct atatggcgaa actgcagaag cctttcattt
     3661 atctcatgaa cttgcgcatt tcaccgcttc tcattttg
//
LOCUS       DAZBTH010000169         3683 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000169 DAZBTH010000000
VERSION     DAZBTH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.174"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..876
                     /locus_tag="ACHGNW_002733"
     CDS             28..876
                     /locus_tag="ACHGNW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6926985.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            976..1410
                     /locus_tag="ACHGNW_002734"
     CDS             976..1410
                     /locus_tag="ACHGNW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6926986.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1590..2000
                     /locus_tag="ACHGNW_002735"
     CDS             1590..2000
                     /locus_tag="ACHGNW_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6926987.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2057..2497
                     /locus_tag="ACHGNW_002736"
     CDS             2057..2497
                     /locus_tag="ACHGNW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6926988.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2600..3679
                     /locus_tag="ACHGNW_002737"
     CDS             2600..3679
                     /locus_tag="ACHGNW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6926989.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT         1092 a          614 c          697 g         1280 t
ORIGIN      
        1 ttattaatga attaaggaag tgaataaatg aaagaaacga gacaatttat atggacatac
       61 attaagatta tttttgccct cttcatatta ggagtcgcta tcaatatgtt tttagggcct
      121 catcatattg ctgctggagg tgtcagcggt ctaggaatat tactggaagc tgctttaggc
      181 tttgaccgag ctatagttat tttagtgttg aatatcgtta tgttggtact agctcttcta
      241 tttttaggta aaaaaccctt ttttaaagta cttttcggaa gcttggtttt tcctttaatc
      301 attgcaatag ttccagaaac aatgattact tcagatcggt tgttgtctgt catttttgga
      361 agtgcgattt ttgctttagg agttgcgatt ttatataaaa acaactcttc tagcggcggt
      421 acgacgatcc ctcccctcat ttttaaaaaa tatttccact tgaatacttc gattggctta
      481 ttttttacag acgcattcgt tgtttctctt aatctgttta tttttggaat agaagagttt
      541 ctttatgcta ttttatctat tgcaatcact tcaattgtca tgacttatat cgaaacaggt
      601 atcaatcgaa agaaatctgt tatggtcatg agtgaacatt atttggaaga catccgcctt
      661 cgcttaagtg ctgagattgg acgtggcctt accctgctag aggcaaaagg cgggtataat
      721 cgaaagtcaa aagaggtttt attgatcgtt gtgactgatc atgaattttc ccgaatgaaa
      781 ccattgatag aagaaatcga tccaagtgcc tttgtcattg tcagcagcgt agcagaagta
      841 atgggacgcg gatttactta tcatccaatc gaatgagttg gtaagtaaat ccgctatttt
      901 tttgaatgcg ttttctttag ctggtataat aaaagaatct agtaactgtt cgactgaaaa
      961 ggagaggaga tctttatgca taaaggaacg gaaatcgatt ttgtattaaa tgatagtttg
     1021 aaagcactag aactgtatca aacaatcttt gatgaaatac aactgattga ggcaacagat
     1081 taccctagag gcaataatga agtcattttt accttgtatg gtacgagatt ccatatgctg
     1141 gatgaaaaca cgtctcttgg tttgactgct cctactgctg aacatccaaa tacgatctgg
     1201 ttcaatatca tcgtctctga tatcgaggac acacataata aagcattacg aaatggctgt
     1261 gtagagattc aagaaatgac agaaatgatg gactttggtg tgactaactc aatgttcaga
     1321 gatccttttg gttatttatg gatgcttcat caggtccatc gtgaagtaat ttttgaagaa
     1381 agaaaacaac tttttgagaa atatatgtaa tgtttatgat gagattattt tttgtctctg
     1441 atattttttg tatatgtttg ctattcgaga aagcgcgtct gtttcatgtg ctttctctta
     1501 tttatcttat gccaatccat ttctttgctt tccacaatta tattgtgtaa aattcaattg
     1561 tataaaatat aattaaaggt ggtaatttta tgaaaaaaat gtattctacg aaaatgatca
     1621 acacaggcgg acgctctgga gaagtgcata atcccgatcg tacattcgaa ttgaatattg
     1681 cagctcctgg taaacgcgtc gaaaatgcaa ccaaccctga acaattgttt gcagctggtt
     1741 tcagtgcatg ttttaacagt gcattagaat tgatcaaaca gcaaaaaaat atctctgggg
     1801 cttctactgt cagtgcacaa gtttcccttt attcagaaag tgaaacgagc tttgtcttag
     1861 gtgtagagat tgaaggatct atcgaaggtc tttctctgga agaaacgcaa gaattattag
     1921 aggcagcgca taaagtgtgt ccgtattcaa aagctacagc tggaaatata gaagttactc
     1981 tgaaagcagt tagtgaatag acttttttgt gtaaatccgc tatgatacac tatagtaaaa
     2041 cagcggaggt ggaaaaatgg aagatctata tttagttaat caagtatgtt ttcctttata
     2101 tgccgcctct aaagaaatca ttagacacta cactcccctc ctcaagccgc tgaatcttac
     2161 ctatacccag tatcttgtga tgttagtttt atgggaaaag aaaaagactt cagtgaaaga
     2221 gctaggaaat gatttgtgtc tggattctgg tacgctgact cccttactaa aaaagttagc
     2281 agaaaaaaat tatattaatc gtcagcgtga aacgggtgat gaaagaatcg taaatgttac
     2341 gctgactgct caaggtgctg cgttgcaaaa acaagcagaa ggtattccac aaaagatcaa
     2401 gtcttatctt ccacttgaac aggaagaatt ggatacactg caacatctga cccgaaaaat
     2461 gctagataat tttcatcaag accagaaaaa gggctgaaac tgctagatga acgattttat
     2521 gaggaaaacc aactcataag ataacgtcat ttagcggttt ttctttttgc tctttcttat
     2581 tttcatattc catcatataa tgaataacgt gtttattttt tgttatttgg aggttgtcga
     2641 tttgaccgtt tatcagcgtt ttttggcgaa tgaaaaaata cgccgtctca ctgttttatt
     2701 atttgtcgtt tttgttcttt tcttagttag aagtatgatt acaaccattt tgctcacatt
     2761 tatctttact tatttgatga ttcatcttgt tcaaatcatt cagcgatttg tccgaatccc
     2821 tacaggtgtt ttagctatca tgatttatac actgatcgtt taccttatct atctggcatt
     2881 cacaaagtat atcccgatgc ttattcatca aacatttgac atgatccagt ccgttattaa
     2941 tttttacgag catcagccaa aagatgcaga tcctgtttta caatatatcc atacttatat
     3001 tgagcgaaac gatttttttg aacagcttca aaatggcgct tcgattgcgc ttggctattt
     3061 acaggatata ggaaaaatgg cagtggcgtt tgctatgtca ttcatcttaa gtttcttttt
     3121 tatgattgaa aagaaaaaaa caattgtatt ctcaagattg tttttaaaaa gtgaattttc
     3181 ttggttcttt caagatattt actattttgc cgataagttt gtcaatacat ttggtttggt
     3241 attagaagct caatttgttg tcgctctttt aaatacgttg ctcactacaa tcgccttggc
     3301 tgtatttggc tttcatcagc tgctgagttt agcgatcatg attttcattt taagtctgat
     3361 cccagtagca ggtgttatca tttcttgtat tcctctatct ttcatagctt attcccaagg
     3421 aggcatccga gatgttgttt atatcctgat tacgattttg attgtccatc taattgagtc
     3481 ttatgtgctg aatccgaagt tgatgtccag caaaacagaa ctgccgattt tttatacatt
     3541 tgtggtctta cttgttagcg aacggttctt tggcgtttgg ggcttgattg tcggcattcc
     3601 gatctttacc ttctttttag atgtattgaa agtgaaaaag atcccgaacc atcttccaaa
     3661 agaaacagat atcccttaaa ata
//
LOCUS       DAZBTH010000170         3567 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000170 DAZBTH010000000
VERSION     DAZBTH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.175"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..2344
                     /locus_tag="ACHGNW_002738"
     CDS             155..2344
                     /locus_tag="ACHGNW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6926990.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>3567
                     /locus_tag="ACHGNW_002739"
     CDS             2341..>3567
                     /locus_tag="ACHGNW_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN6926991.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1350 a          481 c          593 g         1143 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tatttta
//
LOCUS       DAZBTH010000171         3553 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000171 DAZBTH010000000
VERSION     DAZBTH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.176"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..211)
                     /locus_tag="ACHGNW_002740"
     CDS             complement(<1..211)
                     /locus_tag="ACHGNW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6926992.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENK"
     gene            complement(509..1483)
                     /gene="sgrA"
                     /locus_tag="ACHGNW_002741"
     CDS             complement(509..1483)
                     /gene="sgrA"
                     /locus_tag="ACHGNW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN6926993.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1679..2518)
                     /locus_tag="ACHGNW_002742"
     CDS             complement(1679..2518)
                     /locus_tag="ACHGNW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6926994.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2703..>3553
                     /locus_tag="ACHGNW_002743"
     CDS             2703..>3553
                     /locus_tag="ACHGNW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN6926995.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVT"
BASE COUNT         1181 a          732 c          527 g         1113 t
ORIGIN      
        1 tcttgttttc tgaaagaaaa gataatttat attctggata cctttcgcaa aacgattctg
       61 cttgttgaat caccgaattc atcttagcca gggtgtagtt tagatctaaa gcgatatcct
      121 tcaccacata taatttctcg tctctttcat aacaatagtt caaaaattga tatacttgcc
      181 tttcattgat atcaataaat tcttcaaaca aagtacatac tcctttttat ctattattta
      241 gacttattcc gacagctaca tgaataacgt acaaatagat tcgttactga tttttcagag
      301 atactaccat gcgcagtgtg cattgtcaat tattcttgct cttattttcc atttgtgttc
      361 ctgttatttc ctgtttctca aatatgttag taaacaaaaa tagatctggc aaagctacaa
      421 cttcgccagg caatacagca ctctcgaatg aaatattcac tcaaaaatct atcgttttat
      481 tcaattttag atctgttgct tttattcttt atttcactcg atggaagaga gaacgaaaat
      541 agcttgctcc tacaatcaaa atgagtaaca cacccatcca tgtaattagc ttgttcaagg
      601 ttctactacc agtttctggt aaatcgcttt ttctattatc tgtttctttc gctttttctt
      661 gatgatcaat tccacgagaa acaaaatcag gctgttttag cacacttttt aggttgctcc
      721 ctgcatttct cttttgttcc ttagttggta tgataggtac caaatcaagt tcattattta
      781 cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc
      841 ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg cttcctgtgc
      901 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgttttct gtgctacttt
      961 cactgctcgt actctctgta ctgctttcac cgctcgtgtt ttctgtactg ctttcactac
     1021 ttgtgctatc cgtactgctt ggcggtccaa caactgtcca actgcttggt ttcaaattct
     1081 ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc aactttatct ttactcttga
     1141 tctcatcaat cgatagttct gcagaaatca ttattgtata ctcaatacca gcggtccatt
     1201 gtacgtcttt taaataaact tccccaataa ctttttctga acttccatta ccatcccaac
     1261 catatgttag aacagcatgt acatcactag cattcacgct tttgtttggt ttgactggaa
     1321 tagtaatgac ttgttgtgtc atgtcataaa caatccgatc agttttcaca gtaaatgaaa
     1381 gattctcatt tgcccgttca tttgcagaag tactgattcc aatcattgct gcaattatac
     1441 tcagcccaag aataacaaat aatacggtcg ctgttttctt catttctatc actctccata
     1501 taattacctt ctatttgata aaggaattat ataaaatgaa aatgatgaga acacgacagt
     1561 cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt gaatttgttt actttttcgt
     1621 ttaatcaatg acagacttaa aaaagcaaga ccgatatcag ccttgctttc ttcatctttt
     1681 attgataaac agcagtgtat ttcttcatcc gctttgtttt atatgcatag agtaacaaac
     1741 caacaatcaa gactaacaag aataatgtag ttgatataga tgcttcagcc ccaaagtttc
     1801 ccccagtaat tattgtattt tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg
     1861 ttccgctgac tggtataccc aaaatagctc cttgtgtcca attccaagta atatgagcac
     1921 caatagccgt aaataaactt cctgtactgt agataagtgc gctgaaaaag caacctgcta
     1981 aaaagatatt gaaaactgcc agaaatgtca tgttaggatt caatccgtgc aaaagagcaa
     2041 ataatgcagc agatccaatc attgctatta acggaccaaa tctaagcatc tgatgctgaa
     2101 aaaagccacg gaataaaagc tcttctgatg cccccactgc aataaataaa ataaaactcg
     2161 ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg atacgccccc gtcaaatata
     2221 atggactagt aataagcaga aacactccaa ttccaacaac gagacctccc gctaaatacc
     2281 cccagtcttt tcctcgaaca gaaacaccat aatcttctat tctttttcct tcgaccttac
     2341 tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc
     2401 caatccattc actatttcca ataaacatat ccgcaagtgc attattgaca agaattccta
     2461 aaacaaaacc aactaagact attaaaacag atccaatcaa ccgaataatc ctgatcatct
     2521 ttcatccctc ctttatatta agtttaacaa cccttttctt tcttgtctcg tgaaaactat
     2581 gtaaatgttc tccatcatag agagaataga tttttacgaa ctaagaatat ctacagcaac
     2641 cttacgaaaa tgaaagactg tctccctaat tcttctgtta gactgttttt aataaggaaa
     2701 ggatgggaaa ttttttgaaa aaacaactac taattatgtg cctttcagca tgtgcaattt
     2761 tactattctc aggttgtagc aagtccaatg aaacgattgc aacgtttaaa ggtggaacac
     2821 ttacagttca ggatttttat gataacccaa aagttaaatc aaataatcaa acactattaa
     2881 aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt tcttcaaagg
     2941 aagtaacaaa agaatataat gaacaaatca aaaaactcgg tccaaactac aaggaacaat
     3001 taaaagcagt tggccaaaca gaagagacat ataagaaact ctttaaacag atgctggctt
     3061 tccaatacgg tttgaaagcc aatgtaaaat taacagataa agatctagac actgcttgga
     3121 aagaattcta tccagaagtt agtacacaga tcattctatt ttcgactaaa gaagaagcag
     3181 ataaagcaaa aaaagaagca aatgaagggg aaaatttctc caagcttgtt caagcttatg
     3241 gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa ttttgattca accaatccag
     3301 agattcctac tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa gtttctgata
     3361 tcattcctgt aacagatcca acgacttatc agcaaagcta ttatctagta aaaatggtaa
     3421 aaaaacagga taaaggttct aataaagaca aatacaaatc tgagctagaa aaaattgcta
     3481 ccgaagcaaa aactcttgat acggaattca tggataaaac aatccgtaaa gtgatgaaaa
     3541 aagacaatgt aac
//
LOCUS       DAZBTH010000172         3480 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000172 DAZBTH010000000
VERSION     DAZBTH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.177"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHGNW_002744"
     CDS             complement(<1..2232)
                     /locus_tag="ACHGNW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6926996.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHGNW_002745"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHGNW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          978 a          770 c          548 g         1184 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
//
LOCUS       DAZBTH010000173         3410 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000173 DAZBTH010000000
VERSION     DAZBTH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.178"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..1200
                     /locus_tag="ACHGNW_002746"
     CDS             160..1200
                     /locus_tag="ACHGNW_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6926997.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2024..2356
                     /gene="traE"
                     /locus_tag="ACHGNW_002747"
     CDS             2024..2356
                     /gene="traE"
                     /locus_tag="ACHGNW_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN6926998.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2975..3316
                     /locus_tag="ACHGNW_002748"
     CDS             2975..3316
                     /locus_tag="ACHGNW_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN6926999.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1267 a          461 c          646 g         1036 t
ORIGIN      
        1 ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa
       61 cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat
      121 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
      181 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      241 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      301 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      361 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      421 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      481 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      541 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      601 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      661 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      721 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      781 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
      841 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
      901 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt
      961 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
     1021 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
     1081 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1141 gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga
     1201 aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta
     1261 ttattctaat tatattacga atattacaaa ataatagcta ttttaataga atttataagt
     1321 taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat
     1381 aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat
     1441 aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact
     1501 tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc
     1561 tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt ggacaattga
     1621 aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
     1681 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
     1741 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
     1801 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
     1861 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
     1921 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     1981 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
     2041 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
     2101 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
     2161 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
     2221 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
     2281 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     2341 gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     2401 tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac
     2461 ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt
     2521 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     2581 aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc
     2641 tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc atggaataag
     2701 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     2761 tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta
     2821 gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac
     2881 gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata taaagcatag
     2941 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     3001 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac
     3061 gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt
     3121 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     3181 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     3241 agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc
     3301 ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc
     3361 acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
//
LOCUS       DAZBTH010000174         3266 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000174 DAZBTH010000000
VERSION     DAZBTH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.179"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            588..1004
                     /locus_tag="ACHGNW_002749"
     CDS             588..1004
                     /locus_tag="ACHGNW_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN6927000.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="ACHGNW_002750"
     CDS             1018..1584
                     /locus_tag="ACHGNW_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN6927001.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="ACHGNW_002751"
     CDS             1607..2593
                     /locus_tag="ACHGNW_002751"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6927002.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3266)
                     /gene="scm"
                     /locus_tag="ACHGNW_002752"
     CDS             complement(2748..>3266)
                     /gene="scm"
                     /locus_tag="ACHGNW_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6927003.1"
                     /translation="ASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDST
                     DTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTR
                     HASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGL
                     RITFMSYKKSKR"
BASE COUNT          979 a          612 c          696 g          979 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgctatcact tgtggtagag
     3241 gtagtatctg tactgtcact tgaagc
//
LOCUS       DAZBTH010000175         3221 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000175 DAZBTH010000000
VERSION     DAZBTH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.180"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHGNW_002753"
     CDS             301..585
                     /locus_tag="ACHGNW_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927004.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHGNW_002754"
     CDS             588..1004
                     /locus_tag="ACHGNW_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927005.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHGNW_002755"
     CDS             complement(1141..1860)
                     /locus_tag="ACHGNW_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6927006.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHGNW_002756"
     CDS             2544..2630
                     /locus_tag="ACHGNW_002756"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6927007.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHGNW_002757"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHGNW_002757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927008.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZBTH010000176         3219 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000176 DAZBTH010000000
VERSION     DAZBTH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.181"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..494
                     /locus_tag="ACHGNW_002758"
     CDS             144..494
                     /locus_tag="ACHGNW_002758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6927009.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="ACHGNW_002759"
     CDS             908..1726
                     /locus_tag="ACHGNW_002759"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6927010.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="ACHGNW_002760"
     CDS             complement(1799..2158)
                     /locus_tag="ACHGNW_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6927011.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
     gene            2899..>3219
                     /locus_tag="ACHGNW_002761"
     CDS             2899..>3219
                     /locus_tag="ACHGNW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6927012.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         1111 a          468 c          565 g         1075 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtcgcttct gtagaataaa gttaacgaca accaattcac
     2881 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
     2941 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
     3001 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
     3061 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
     3121 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
     3181 agaacaaacg atacattgga aaccactatt atccattta
//
LOCUS       DAZBTH010000177         3214 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000177 DAZBTH010000000
VERSION     DAZBTH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.182"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            473..916
                     /locus_tag="ACHGNW_002762"
     CDS             473..916
                     /locus_tag="ACHGNW_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927013.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNG"
     gene            970..1707
                     /locus_tag="ACHGNW_002763"
     CDS             970..1707
                     /locus_tag="ACHGNW_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927014.1"
                     /translation="MEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLK
                     RIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNL
                     GITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHS
                     DILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTL
                     TYPDTQMIFLLVPMIVSWVDNQQRISE"
     gene            1791..2879
                     /locus_tag="ACHGNW_002764"
     CDS             1791..2879
                     /locus_tag="ACHGNW_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFN6927015.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2876..>3214
                     /locus_tag="ACHGNW_002765"
     CDS             2876..>3214
                     /locus_tag="ACHGNW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN6927016.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYG"
BASE COUNT         1163 a          331 c          552 g         1168 t
ORIGIN      
        1 ttaacaacga tttttttgta tacaaatacg atatgctcat taattattgg aatttgtagt
       61 gctgtttaaa ttaatgaagg tgtaattata tgagtgatca ttttgaaaaa gtttgttgag
      121 aagatgtgct tctagtcatg tgatgggaga aaataattta actgttagag tgaatagtta
      181 aattattctg gttatatgaa aaaagtagtt tctaaaataa tcttgcaggg attaacttaa
      241 agaaattgag agaatagttt ctttgaaatt tctatataca atgagaagct gaaatttact
      301 tttatttgat gactggaaat agaaattaaa tatattgtat tgttacatta aagaaaactt
      361 gtatggaaaa atcagtacaa taccattatt taatgttctt cacttaaagc gcaaaaaata
      421 tcagtttatt aaactcgtat atagaacaaa tatatgtaga cggggcgaaa cgatgagtgt
      481 aaaaaaaatg ctatttatgg taagtacaat aattgtatta acatcccgat tttggggaat
      541 taatactagt ttatctatgc gatattttat tatggcaata tgggatttat attttatgtt
      601 aagctatttt tcttatcgtg agaaaacata taaaaaatac gttatcaaga ataacttttg
      661 gttgactata atgccgtttg tgatattttc tatctatact ctaataatat ggggaattgg
      721 ttcaaatgca gaatttgaaa attttataaa gttatgttcc acgttgcttt atttaattct
      781 tgcatatggt tacgcatgta cagcatttta cttatttaag gcagaaggaa ttgattttat
      841 tttttggtca ggtgttattt catatatgag tggttcagtt ttttatttaa ttgtatctta
      901 tggtttaaat ggttaatttc ttatataaaa agtcttataa caggtgaaac aaatactgca
      961 aattttgcta tggaggtaca tgatttaact tttgcgatgg gggttttttt cctttattac
     1021 gtattttttg aaaataaaaa tacaaaaaaa cataagataa aaattatcgt atctttaatt
     1081 ttaattttca tgggattgaa gagaattgaa ctgttagctt taagtggtgc aattctagtg
     1141 tattatgttt tattaaagtg gggaaaaaag attcaaatcc gtgcattagt gtgtactatt
     1201 tgtgcaactg ttatttcgtt agtgtttatt tacattatag atacaggaat tttggcattg
     1261 cttatgtcaa atttgggcat tacagatgac ggtgcgaggc tttcatttta tctatattca
     1321 tctaaatttt atgaacttgg tttaggatat gtaggaacag gatggacatg gttttcaaaa
     1381 tactttttta atctttattt atcgcacttt aggttaaatg gtggaagaat tgcagcaagc
     1441 ctacacagtg atatattaac tatttatata gaagttggat gtatagtttt tattatatgg
     1501 atgttttggt tattttttgt gaaaccgata agactaacta aaaaatttgg attgaaaaca
     1561 ggggagtgtg tattattatt aactatatat atgtttatat tatatttgac ggataatacg
     1621 ctcacttatc cagacactca aatgattttt ctcttagtac ctatgatagt ttcgtgggtt
     1681 gataatcaac aacgaatatc ggaatagata gctaaagtat attttaatta tgaatttata
     1741 attagttgta aaagttaata tttaacggac aggaagtagg gtgaaaaaat ttgaaaaaaa
     1801 ttttagtgtt agcaaataca tattatcaaa taatagtagc aatacaaatg aaaaatacaa
     1861 ttttttccga tgagtatgtt gaaattatta tttctgatca ttctaaaaac actaaagaaa
     1921 ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg tcattattta gaaagcaaat
     1981 cttttgatga taatattggg tattttcaaa agattaagga ctgtcttcag atttcttgtg
     2041 gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa aagtgaatat tttgatgaat
     2101 ttatttgttt ttcaatggga gaggtttggt tggaaggaat atatgcttgc ttatatcgaa
     2161 ataataaaaa tgtacaggta tcaattttag aagaaggtat tttaagctat ggagttgaca
     2221 ttattccaac agcaagaaca aaagctatcg cagtactcaa ttacttaaaa aaaaggactc
     2281 cactatataa caaaagctat aatttttatt gtttttttcc taatatatat acaggaagag
     2341 agaatacaat acaaattcca ttagttacag ctgggtcaag aaccgcattg caactttcgg
     2401 atatttttaa ccctatgata gatatttata aagaaaaaaa atatatattt tttacgtctg
     2461 tttatgattt tgagggcgga gaacctgtcg gagaatatga attagtatgc aaaatagcag
     2521 acttagtagg aaaagataat ctaattgtta agacgcatcc tagagatgta agaacaattt
     2581 ataaagataa tggatttatc gaggataaaa attcatcaat accttgggag gcgattcaat
     2641 tgtcaggcga ttttaacgat aaaatattta tgacaattaa ttcaggaagt gttatttcag
     2701 gtagtacaat gtctgcaaaa cctgtacgca cgtattatat gtataaattg tgtaacatag
     2761 aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga gcgattgctt aagcaaaaaa
     2821 cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag cttagatgat atactatgag
     2881 caaaactaaa tatttaatta aaaatatggg aatattaaca ataagtaatt tttcttcaaa
     2941 aattttagtt tttttgttag tccctttata tacaagtgta ttatccacaa cagaatatgg
     3001 cacatatgat ttgacaattt caacagccac attattgttt ccaattttaa ccttaaacat
     3061 tgtagatgct gtgatgcgct ttttaatgga taaagataaa aataaaaaaa atatagcatc
     3121 tatagcgatt aaagttatat ttttaagcgt gatagtcttt ggatccgtaa tgtatttgtt
     3181 aaatattaca aaattatggc aagatgttta tgga
//
LOCUS       DAZBTH010000178         3211 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000178 DAZBTH010000000
VERSION     DAZBTH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.183"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1612
                     /locus_tag="ACHGNW_002766"
     CDS             <1..1612
                     /locus_tag="ACHGNW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6927017.1"
                     /translation="LTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPD
                     GTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDV
                     TPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIG
                     GTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPV
                     EVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGN
                     KKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKE
                     DLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPI
                     TEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDES
                     GNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDS
                     NIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1746..3089
                     /locus_tag="ACHGNW_002767"
     CDS             1746..3089
                     /locus_tag="ACHGNW_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6927018.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1272 a          448 c          620 g          871 t
ORIGIN      
        1 tctaacagat aatgtaacca acctaccaac attaccagaa ggaacaacag taacagatgt
       61 cactcctggt ggtacgattg ataccaacac accaggtaat tacgaagggg ttatcgaagt
      121 aacgtatcca gatggtacga aagatacagt gaaagttcca gtagaagtga cagacaatcg
      181 ttccgatgcc gataaatatg aaccaacagt agaaggcgaa aaagtagaag ttgggggcac
      241 agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa caacagtaac
      301 agatgttact cccggcggta caattgatac caacacacca ggtaattacg aaggtgtcat
      361 tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag aagtgacaga
      421 caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg
      481 aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag aaggaacaac
      541 agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca attatgaagg
      601 tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt
      661 gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag tgactacaga
      721 cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt taccaaaagg
      781 aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata aaaaaggtac
      841 tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg ttacagtgac
      901 agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga ctacagactt
      961 aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac caaaaggaac
     1021 tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa aaggtactgt
     1081 agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt ctgtagtaga
     1141 taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc aaattgttac
     1201 tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa gtcaacacca
     1261 tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag aggcagggac
     1321 aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta ctaatgtagt
     1381 tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta agactgacaa
     1441 tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct caaaagtttt
     1501 tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt taatactgtt
     1561 aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1621 tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt gcttttttat
     1681 gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga aaagaatgta
     1741 attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc gcagctcgat
     1801 ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa tcagaaatta
     1861 gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat ttagaagatc
     1921 ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt ctagagacag
     1981 aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa gatggtacaa
     2041 gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag
     2101 atataccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag
     2161 agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat
     2221 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     2281 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     2341 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     2401 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2461 tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag
     2521 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2581 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2641 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac aatcaagatt
     2701 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
     2761 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatatagaaa
     2821 tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta
     2881 gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat
     2941 ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga
     3001 aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg
     3061 cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg
     3121 tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag
     3181 aaaaaagaga ggtaaatcaa atgaaaaaat c
//
LOCUS       DAZBTH010000179         3129 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000179 DAZBTH010000000
VERSION     DAZBTH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.184"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACHGNW_002768"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACHGNW_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACHGNW_002769"
     CDS             1360..1992
                     /locus_tag="ACHGNW_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6927019.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACHGNW_002770"
     CDS             2200..2424
                     /locus_tag="ACHGNW_002770"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFN6927020.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACHGNW_002771"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACHGNW_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN6927021.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACHGNW_002772"
     CDS             2749..3018
                     /locus_tag="ACHGNW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6927022.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DAZBTH010000180         3084 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000180 DAZBTH010000000
VERSION     DAZBTH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.186"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..302
                     /locus_tag="ACHGNW_002773"
     CDS             <1..302
                     /locus_tag="ACHGNW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN6927023.1"
                     /translation="IGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEI
                     ETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            275..952
                     /locus_tag="ACHGNW_002774"
     CDS             275..952
                     /locus_tag="ACHGNW_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN6927024.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            903..2084
                     /locus_tag="ACHGNW_002775"
     CDS             903..2084
                     /locus_tag="ACHGNW_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN6927025.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2097..3074
                     /locus_tag="ACHGNW_002776"
     CDS             2097..3074
                     /locus_tag="ACHGNW_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6927026.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1146 a          389 c          595 g          954 t
ORIGIN      
        1 tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa
       61 atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa
      121 atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct
      181 tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat
      241 tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt
      301 aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat
      361 tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa
      421 aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa
      481 aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat
      541 aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt
      601 ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc
      661 agatgctgta attggagagc atgctataat aaacacaggt agcattgttg agcatgattg
      721 tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt
      781 aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc
      841 ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc
      901 ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat
      961 tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata
     1021 attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata
     1081 ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct
     1141 taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag
     1201 ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta
     1261 atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca
     1321 accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac
     1381 gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt
     1441 taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat
     1501 ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa
     1561 aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc
     1621 aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg
     1681 gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata
     1741 aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc
     1801 caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta
     1861 acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac
     1921 atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt
     1981 cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg
     2041 aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga
     2101 acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac
     2161 actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg
     2221 aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa
     2281 taaattcagt ttttgtttgt acatccccta taacccatga atttattata aaagaggctt
     2341 taaatttaga ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa
     2401 taatcgaagt agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga
     2461 gaaaagaaat tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat
     2521 accatgtagg acagtattta cccgactggc atccttggga agattatcgc aatttttttg
     2581 tttcaaacaa aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta
     2641 tagatacatt tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg
     2701 atattgatta ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa
     2761 ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa
     2821 agattttaat agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt
     2881 ctagtatgac agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata
     2941 ctattattga agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga
     3001 aaatggaacc ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca
     3061 ttgaggtaaa ttaatgatga aagt
//
LOCUS       DAZBTH010000181         3072 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000181 DAZBTH010000000
VERSION     DAZBTH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.187"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1574
                     /gene="gadC"
                     /locus_tag="ACHGNW_002777"
     CDS             63..1574
                     /gene="gadC"
                     /locus_tag="ACHGNW_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN6927027.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1587..2987
                     /locus_tag="ACHGNW_002778"
     CDS             1587..2987
                     /locus_tag="ACHGNW_002778"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN6927028.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT          942 a          504 c          617 g         1009 t
ORIGIN      
        1 ataagcgatt ttccataacc ttggtaggaa tgacaaatta aataggaaag ggaagtaata
       61 atatgaataa aaaaaagtta tctctattcg gtttttttgc cctgacagct tccatggttt
      121 tgactgttta tgagtatccg acctttgcaa catcacaatt acatttggtt ttctttcttc
      181 ttttgggagg tattttatgg tttctaccag ttgctttatg tgctgcagaa atggcaacag
      241 ttgaaggatg gaaagatggt gggattttca gttgggttag tcaaacttta ggagaacgtt
      301 ttggttttgc agctattttt ttccaatggt ttcaaattac agtaggtttt attacaatga
      361 tctacttcat actaggagcg ctgtcgtatg taattaattt tccagagctt aatacagatc
      421 catttatcaa gtttattggt gtattagtca ttttctgggt actgactttt tcacagttgg
      481 gcgggaccaa tcgtaccgct aaaattgcta aagcaggatt tgtcattgga attgttattc
      541 cttcgatcat tctctttggt ttagcagcag cctactttat cggcggtaat ccgattcaaa
      601 tggctattaa tgcgaaagct tttattcctg acttttctca gctttcaact ttagtagtat
      661 ttgtatcttt tatattggct tatatgggcg tagaagcttc agcatcacat attaatgagc
      721 tggaaaatcc ccaaagaaac tacccattgg caatgttttt actagtgatt ttggccatcg
      781 ccttagatgc aatcggcggt ttctcagtag cagcggttat tccacaacat gacttgtctc
      841 tcagtgctgg tgtaatccaa acatttgaaa cgcttatttt acattttaac agtcatttac
      901 attggttggt aaaagtgatt gctctcatga ttgcctgtgg cgttatgggt gaagttagtt
      961 cttgggtagt tggtccatca cgagggatgt ttgcagctgc tcagcgaggt ctgttaccag
     1021 ttttcttccg gaaaactaat aaacaagacg ttccagttcc gttgattatg atacaaggaa
     1081 ttatcgttac tatttgggca gcagtcttaa cttttggtgg aggaggcaac aatttatcat
     1141 tcttagtagc aatctcctta actgttgtca tttatctcgt aggatattta ctatttttcc
     1201 taggttactt tgtattagtt tttaagaaaa aagacttaaa acgaacttac aatgtaccag
     1261 gaaaaactgt tggcaaaacg attatcggtg gaattggttt tattttatca gtgttcgcat
     1321 tgcttatctc ttttgtacca ccagcttcaa ttgcagctag tgagcaccga acataccaaa
     1381 tcatactact tattagcttt gtagtgactg ttcttttacc ttttatcatt tatgaagtac
     1441 atgataaaag tgctcacaca actattgagg aacctactca cgtgaaagct gaagacgtta
     1501 atcccgctgt ttatccagct gctagaggag agcaccacgt tacaaaaaat gatgaacata
     1561 ttttaaaaca ttaggaggaa ttcattatgt tatacggaaa agataatcaa gaagaaaaaa
     1621 actatttgga accaattttt ggctctgcaa gtgaggatgt tgacttgcct aaatataagt
     1681 taaacaaaga atccattgaa ccacgaattg cttatcaatt agtacaagac gagatgttgg
     1741 atgaaggaaa tgcgcgatta aacttagcta ctttttgtca aacgtatatg gaacctgaag
     1801 cagtgaaatt gatgacccaa acgttagaaa aaaatgcaat tgataaatca gaatacccac
     1861 gaacaacgga aattgaaaac cgctgtgtaa atatgattgc tgatttatgg catgctccaa
     1921 ataatgaaaa attcatggga acttcaacga tcggctcttc agaagcctgc atgctgggtg
     1981 gtatggccat gaaatttgct tggcgtaaac gtgctgaaaa attaggtctt gatattcaag
     2041 caaaaaaacc taacctggtg atctcttctg gttaccaagt ttgttgggaa aaattctgtg
     2101 tatattggga tgtggaactg agagaagtcc caatggatga aaaacatatg tcaattaatc
     2161 tagatactgt catggattat gtggatgagt acacaattgg tattgtaggt attatgggta
     2221 ttacttacac tggtcgttat gatgatatca agggtctgaa tgatttagtt gaagctcaca
     2281 ataaacaaac tgactataaa gtatacattc atgttgacgc tgcatcgggt ggcttttatg
     2341 caccatttac tgaacctgat ctagtttggg attttcaatt gaaaaatgtt atctcaatta
     2401 attcttcagg tcacaaatat ggtttggtat atccaggtgt gggttgggtc ttatggcgtg
     2461 accaacaata cttaccagaa gaattagtat ttaaagttag ttacttaggt ggagaaatgc
     2521 caactatggc tatcaacttc tctcatagtg cagcacaact aattgggcaa tactacaact
     2581 ttgtacgcta tggctttgat ggttatcgtg atattcacca aagaactcat gatgttgctg
     2641 tttatttagc caaagagatc gaaaaaactg gtatttttga aatcattaat gatggatcag
     2701 aattgccagt tgtgtgctat aagctgaaag aagatcccaa tcgcgaatgg acactatatg
     2761 atttatctga tcgtctgtta atgaagggat ggcaagtccc agcctaccca ttacctaaag
     2821 acttggatca attaattatt caacgcttag ttgttcgagc agactttgga atgaacatgg
     2881 ctggtgatta tgtacaagat atgaaccaag caattgaaga gttgaataaa gctcatattg
     2941 tttatcataa aaaacaggat gtgaaaacgt acggctttac tcactaaaat tcttcagaga
     3001 aatataaaag aagttaatta actaataaaa ggagttgtga ttaccacaca gctccttaaa
     3061 tctatttgaa ta
//
LOCUS       DAZBTH010000182         3057 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000182 DAZBTH010000000
VERSION     DAZBTH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.188"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(323..685)
                     /locus_tag="ACHGNW_002779"
     CDS             complement(323..685)
                     /locus_tag="ACHGNW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927029.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(759..1139)
                     /locus_tag="ACHGNW_002780"
     CDS             complement(759..1139)
                     /locus_tag="ACHGNW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN6927030.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1221..2396
                     /locus_tag="ACHGNW_002781"
     CDS             1221..2396
                     /locus_tag="ACHGNW_002781"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN6927031.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2473..>3057)
                     /locus_tag="ACHGNW_002782"
     CDS             complement(2473..>3057)
                     /locus_tag="ACHGNW_002782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6927032.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          953 a          584 c          502 g         1018 t
ORIGIN      
        1 tatcttatca tgtcgttagt aacatgtcaa taactatttt ttaaattttt tagaaatgtg
       61 atataacatc cacttcagaa tcaacgctgt taaatttatc atgtatttct tccttggtca
      121 actataaatt aagcattact ttgtgaaatg atttttcgat caattattat tattcgttat
      181 acaaccgttt tcaacatatt ttgaaaataa aatgcactaa aaaactatct ttagcagaac
      241 atttcattgt attttttgga ccaaacaata aaaaaagaag tttgaaaaaa attcaaactt
      301 ctttgttcgc ttaatctgta ttttagaaga aaggcactcc ctctacttcg taagttccaa
      361 gaacagagcg catttgacct ggttcttccc aaatatatcc ttcgcctaat tcttctcttt
      421 gattcttttg ttcagtttca aagacaaaca acggaacatt ttcctcttta atatgtgtat
      481 ttgtcaattc catcaaacta atcttgctaa catcgatatg aactatttct tttaagaaat
      541 taagtatatt cgctaaacca gtctttcctt cagaaagttc cgctaacgca aattccaact
      601 tgtcatcaat tgaatggacc aaaaattttt tagtaccatc ctccaaatgc aacataattg
      661 taccagcaac tcttttgctt gccaaaattt ccacctcgat caacttgttc tatggcttac
      721 ataaaaattc tagcacattt tcagaaaaaa tacacttttt atttcttatt ttctttccgt
      781 tctagttcag ataatgcttc ttctatccaa ataggtaact gctccgcaat agtggtaaat
      841 ccgatcttac gatttttatt cgtgaaatac ctttttctgc gatatggaac agctggattt
      901 gttttttcta acgttcttaa agataaactg atttttttgg aatattcatc aatgtcgatg
      961 atctgaactg ttactggctg tccgactttt aacaatgaat ggatattttt cgtataaccg
     1021 gattgtactt ctgaaacatg aataagtcct tgcgtctcgt tatcaagaga aacgaatgcg
     1081 ccataaggct gtatccctgt cacttttcct tcaattattt gtccaatttt ataagtcatc
     1141 tacttccctc tcttcgcatt tatcattata ctgtaaatag ctcaaaaaaa gaagcgacga
     1201 aaatgactgg aggtcttaag atgatcgaat ttgataaaca gatttcaaga aaagatacag
     1261 aaagtgtaaa atgggacgca attgctgaga catatcaaat ggatgactta cttcctttgt
     1321 gggtagcaga tatggacttc ttgtctcccg gtggggtagc taaagcgttt agtgaataca
     1381 tcaaacatgg aatctttggt tatgccactc tgtctgataa actatatcag gctatcattc
     1441 attgggaacg ccaacaccat gctgtggagt tgacgaaaga aaatatcgtt tttacaagtg
     1501 gtgtgttaac cagtttagct gcagcagtac aaacttttac aaaaccaggc gattctattt
     1561 tgattcatga cccagtttat cctccatttt cctctattat agaaacaaat caacgtcaca
     1621 tcgttcgaag ctctttgcta gaaaaaaata atcatttcat catggacttg tctgacatgg
     1681 agaaaaaaat cgaagaaaac aatgtaaaag caatgatttt atgcaatcct cacaatcctg
     1741 gagggcgtgt ttggagcaaa gaagaattga aacagttaag cgaattgtgt ttaaaacatc
     1801 aagtgattct gtttagtgac gagatccatc aagatttaac tttattcgat catacattta
     1861 cttccatgct aacgattgat cctaatttag atgatctact agttgctttt acttccgcaa
     1921 ccaagacttt caatctagct gcaatcaaaa attcaatggt atttatcaaa aatccagaga
     1981 tgaaagctga ttttgaaaaa catttagtga tgaaccaaca gcacgaaatc aacacttttg
     2041 gactgattgg aacccaagcg gcttatgaga cgggtggaga atggttaagc caactgattc
     2101 cttatctaga aaaaaatgtt caaacagtta atcagttttt tgaacagtat cttccgaaag
     2161 tgagaataat gaaccctgaa ggaacttact taatgtggtt agattttacc gcctatattt
     2221 cagacgacca agaattggag aacactcttg ttcggaaagg aaaagtcgta ttaaatcctg
     2281 gcatcacgtt tggtccttcc ggtcatgggc atatgcgctt gaatattgct tgtccagaag
     2341 aaactttact ggaaggactt aatcgaatca agtatgcttt agagacaatc gattaaatca
     2401 tttattcaaa ataaaaggac gagaccttac taggtttcgt cctttttcat aattactccg
     2461 taccatacca aactattctg aaatttcaat tgtttcaatc actacatcat gtacaggtcg
     2521 gtcttgtgga ccacgttgta catttgcgat ttcatctact gtgtccatcc cttctagtac
     2581 atgtccaaag actgtatgtc ggaaatctag ccaaggagtt ccgccacctt tatacgcttc
     2641 aatgatttct gatgggaatc cagcaccttc tagttgtcca agcatattag ctggcacatt
     2701 tgtgttattt acaataaaga attggcttcc gtttgtgttt ggacctgaat ttgccataga
     2761 taaagctccg cgaaggttga acagttcacg actgaattca tcttcaaaac tttcgccata
     2821 aatactttcg ccacccattc ctgttccagt tggatcgccg ccttggatca tgaaatcagg
     2881 aataacgcgg tgaaaaatca cgccatcata ataacctttt ttggaaagtt caatgaaatt
     2941 ttgaaccgtc ttcggtgcta gttcagggaa taattgaaca gtaatgtcac cacggtttgt
     3001 tttgataaca gcttttggtc ctttttcact ttttaaatct agttgtggga atgccat
//
LOCUS       DAZBTH010000183         3052 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000183 DAZBTH010000000
VERSION     DAZBTH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.189"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACHGNW_002783"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACHGNW_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACHGNW_002784"
     CDS             complement(612..1160)
                     /locus_tag="ACHGNW_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927033.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACHGNW_002785"
     CDS             complement(1161..2018)
                     /locus_tag="ACHGNW_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6927034.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACHGNW_002786"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACHGNW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACHGNW_002787"
     CDS             complement(2580..2909)
                     /locus_tag="ACHGNW_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6927035.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..>3052)
                     /locus_tag="ACHGNW_002788"
     CDS             complement(2986..>3052)
                     /locus_tag="ACHGNW_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6927036.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DAZBTH010000184         2877 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000184 DAZBTH010000000
VERSION     DAZBTH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.190"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            799..1053
                     /locus_tag="ACHGNW_002789"
                     /pseudo
     CDS             799..1053
                     /locus_tag="ACHGNW_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="ACHGNW_002790"
     CDS             1123..1635
                     /locus_tag="ACHGNW_002790"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6927037.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="ACHGNW_002791"
     CDS             1632..2669
                     /locus_tag="ACHGNW_002791"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6927038.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          508 c          573 g          755 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacaccga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgca
//
LOCUS       DAZBTH010000185         2851 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000185 DAZBTH010000000
VERSION     DAZBTH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.191"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..282
                     /locus_tag="ACHGNW_002792"
     CDS             97..282
                     /locus_tag="ACHGNW_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6927039.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            294..542
                     /locus_tag="ACHGNW_002793"
     CDS             294..542
                     /locus_tag="ACHGNW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927040.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            573..752
                     /locus_tag="ACHGNW_002794"
     CDS             573..752
                     /locus_tag="ACHGNW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927041.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(795..1265)
                     /locus_tag="ACHGNW_002795"
     CDS             complement(795..1265)
                     /locus_tag="ACHGNW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927042.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1352..2050
                     /locus_tag="ACHGNW_002796"
     CDS             1352..2050
                     /locus_tag="ACHGNW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN6927043.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2228..2569
                     /locus_tag="ACHGNW_002797"
     CDS             2228..2569
                     /locus_tag="ACHGNW_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927044.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2562..>2851
                     /locus_tag="ACHGNW_002798"
     CDS             2562..>2851
                     /locus_tag="ACHGNW_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN6927045.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1049 a          409 c          569 g          824 t
ORIGIN      
        1 ctatgtctat atactaataa aaatattagt tattgtcaac gaatattagc taatttttta
       61 gcttacagat tattttttta gaaaggagct atttttatgt ctgagaattt agacttaaaa
      121 attcgagcgg agatgagaaa aagaagaatg actttcaaag aactagctgc gcttatcggt
      181 atttcaggag cttatttatc agatattcta aacggcaatc gtgatggaaa gaaagcacaa
      241 cagcatatcg aaacagtgaa aaaaatattg gacatccgat aggggggtag gcgatgacaa
      301 aactaaaaaa acaagatttt gtaaaaaaat acaattattc tccatctact tatcaacgtc
      361 gaatgtcgga actaaaaaat acagcaattt tctcagcggc gtatgaacgg gtcacaggac
      421 aagaagtttg gatcaataca gaattatacg ataaattttt gtctttcaaa tcctataaca
      481 ggttacgcac aagaaaggta acacctaaag aatttatcga gaagcattta gttgatttat
      541 aaaaaacaga acattttgag aggtgaaggt taatgggtaa attcaacaga gcattggtat
      601 tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcaggcaa gcgttgatag
      661 gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa gctgagtaca
      721 gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga aactagaatg
      781 tatattacta ttactcagtt taattctttc actaatttct ctattgggaa gtttgtattt
      841 ttgatatcaa caattttgtg gatttgggtt ctatttagag taaatatcat ttttgtttgg
      901 tgattaagga gtttattaaa attaattttc cctacttcaa atgcaaggta acaagatata
      961 gcacttttag gaggaatatt tagcggtaca gcttcagagt atattctatt atcatcgact
     1021 gatcctcctt tagcaattaa tttcttaaat ggagtagctg accatctgcg gtttaagtta
     1081 gcggatgaaa gttctaaatt aactaacata gctggttctg ttgaatagtt agaaatgatt
     1141 acttttgtat aaatcatatc tgcagcgaag taagaagcat taagttcaac ttctagttga
     1201 gggcgcattc cttttatttt tatccctgtt aaaaacgtac tcaatataaa acttattatt
     1261 gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac cagtttttaa
     1321 ctaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa
     1381 agaagctgtg actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt
     1441 tttaagagat attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga
     1501 aggtaatgaa ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg
     1561 atttttcttg ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata
     1621 cattgaagca ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat
     1681 tgaagatatg atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat
     1741 gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa
     1801 tatgaaagtt atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa
     1861 aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg
     1921 cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga
     1981 aactgcgatc cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga
     2041 caatgaatag agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat
     2101 actataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa
     2161 taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt
     2221 ttagcatatg gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg
     2281 caagccgaaa attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga
     2341 agaactagct tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta
     2401 tcttatccat ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg
     2461 cttgattgat gcgctagaag agtacgacag agataacttg attgatatct cttattacgg
     2521 accaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga
     2581 caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa
     2641 attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg
     2701 caacggtaac tattaatgag tacaaacact acagggttgt ttctggaact catcaagact
     2761 ttcttgttga ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct
     2821 cagtaacggt taaaggacac acggaaaccg a
//
LOCUS       DAZBTH010000186         2703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000186 DAZBTH010000000
VERSION     DAZBTH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.192"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACHGNW_002799"
     CDS             174..518
                     /locus_tag="ACHGNW_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN6927046.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACHGNW_002800"
     CDS             519..938
                     /locus_tag="ACHGNW_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN6927047.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACHGNW_002801"
     CDS             complement(1030..1380)
                     /locus_tag="ACHGNW_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927048.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACHGNW_002802"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACHGNW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1000 a          489 c          523 g          691 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 tta
//
LOCUS       DAZBTH010000187         2627 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000187 DAZBTH010000000
VERSION     DAZBTH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.193"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..835
                     /locus_tag="ACHGNW_002803"
     CDS             <1..835
                     /locus_tag="ACHGNW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6927049.1"
                     /translation="KNSINFDITAYSSSGKKEFEEITYIQNRDDLDTHYDIAFITNPT
                     FKHLETLNVIVEKVDYIFLEKPAFSESIDLTQYNYDYKNIYVAAPLRYKNIMEVVRNS
                     IQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKSMGGGVELDCIHELDYIINLFGFP
                     KSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINV
                     DLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEE
                     K"
     gene            836..1588
                     /locus_tag="ACHGNW_002804"
     CDS             836..1588
                     /locus_tag="ACHGNW_002804"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN6927050.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1593..2540
                     /locus_tag="ACHGNW_002805"
     CDS             1593..2540
                     /locus_tag="ACHGNW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN6927051.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          940 a          333 c          496 g          858 t
ORIGIN      
        1 aaagaattct attaattttg atataacagc ttatagtagc agtggaaaaa aagagtttga
       61 agaaataacg tatattcaaa atagagacga tttggataca cattatgata tagcttttat
      121 tacaaatccg acttttaagc atctggaaac actaaatgta attgttgaaa aagttgatta
      181 catattttta gaaaaacctg ctttttctga gagtattgat cttactcaat ataattatga
      241 ttacaaaaat atttatgttg ctgctccact gaggtataaa aatatcatgg aagttgttcg
      301 taatagtatt caaagtaaaa aagtatattc tgtaagagct atttgttcta cttatcttcc
      361 tgattggaga aaaggtactg attatagaaa taattattct gcctttaaat ctatgggggg
      421 aggagtagaa ttagactgta tacatgaatt ggattatatt ataaacctat ttggtttccc
      481 taaatcagta aaaaaacagt ttggtaaata ttcgaatctt gaaattaaca gtaatgattt
      541 agcaacatat atattgagat acgatgataa aattatagaa attcatttag attattttgg
      601 aagagttcct acaagaaaat ttcaattgtt tactttagat gaatgtatca atgttgactt
      661 attaaataat aaaataagtt ttgaagaaaa cattgttaaa gaaatgcctg aatctgctaa
      721 tgatatgtat gagaaggagt tgaaatattt ctttgaaaac gtagtaacag gcaaagaaaa
      781 ctggaatgat ttatatcatg caaacgaagt attgaaaata gctgaggaga aataaatgga
      841 aattttattt actatttgtg gtagagctgg gtctaagggg tttaaaaata aaaatttgaa
      901 atatttggca ggaaagcccc tagtccattt cgctttagca atgatagata tctatcaaca
      961 aaaacatcct gaacataatt gcacgacttg tttgaatacg gacagtaatg agttagtaga
     1021 attgacgact aagttaaatc cgtctgtgaa aattgtagaa cgtaaacctg aattagctac
     1081 agatactgta gggaaaattg aagtgatacg tgattcattc ttacaactag aaagagaaga
     1141 taatatattt gatttaatta tagatttaga tttgacttca cctttacgtg aagtttctaa
     1201 tcttgaagaa ttaatagatg aacatctaaa aaatttagat aaagacgtct acttttcagt
     1261 tgtttctgca agacgtaatc cctattttaa tatggtaaaa agaattgaaa atgaagttac
     1321 tatagttaat aaatcaagat acacatctag gcaacagaca cctgaagttt ttgaaatgaa
     1381 tgcctcaatg tattctttta aacctgaatt tctaaaaaaa agtaattata tttttgatgg
     1441 gcaatgtggg ataataaaaa tgagagatta tctaatttta gatattgata gtgaagaaga
     1501 ttttaaatgg ttagaatttt tgtttcctaa atttttagaa gattcttcgg agcttcaaga
     1561 tatctataat tatttatatc ggaggtaaaa ggatgagctg ttatcttatc accggtgggg
     1621 ctggcttcat tggttctacc ttagccaatc atttaggaga agaacatgtg gtaatcgtcg
     1681 tcgatgactt atcaatggga aaagtagaaa atcttgatat gacgaaaaat atcactttta
     1741 ttgagggaga tgtcgctgag aaatccttga tggaagagat catgaaagct aataaatttg
     1801 attatatctt tcatttagca gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag
     1861 aaacacatcg tgtgaatttt gaaagtgtat taatgatttt agaattgatc cgcaagtatc
     1921 aacctgattt aaaacggatc gtgtttagtt cttcagcggc tgtttatggg gatgaaccga
     1981 ctttaccaaa aaaagaagag tccgtgattc gcccattgac gccttatgcg gttgataaat
     2041 tcgctgcaga acaatatgtg ctagattatt gtcatttata tgatgtaccc ggtagcgcag
     2101 ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa ttctccatac agtggtgtga
     2161 tttctattct cgtagatcgc tataagaaac aaatcgtagg agaagcaact tcatttacat
     2221 tatttggtga tggaagccag tctagagact ttgtttatat agatgatgta atccaagcat
     2281 tacttttagt tgcgaatgag gaagctgcac tggggaaaca attcaatgtt ggaacgggta
     2341 aagcaaccac acttttggaa ttgattcatg caatcaatga aattctcggt gtagaattga
     2401 cacttgaata caaagaagag cgttcgggtg atatccatga ctctctagca gatattacta
     2461 agatccagtc aattggctat caaccgaaat atggtatttt aagtggaatg actaattatt
     2521 tgaaaactga aatcaattaa aacatactaa gataagatta gagggtaaat tcaggctctt
     2581 tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg gatagaa
//
LOCUS       DAZBTH010000188         2560 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000188 DAZBTH010000000
VERSION     DAZBTH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.194"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1026
                     /locus_tag="ACHGNW_002806"
     CDS             19..1026
                     /locus_tag="ACHGNW_002806"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN6927052.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1038..2042
                     /gene="neuB"
                     /locus_tag="ACHGNW_002807"
     CDS             1038..2042
                     /gene="neuB"
                     /locus_tag="ACHGNW_002807"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN6927053.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2039..>2560
                     /locus_tag="ACHGNW_002808"
     CDS             2039..>2560
                     /locus_tag="ACHGNW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN6927054.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          933 a          332 c          515 g          780 t
ORIGIN      
        1 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
       61 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      121 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      181 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      241 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      301 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      361 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      421 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      481 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      541 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      601 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      661 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      721 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
      781 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
      841 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
      901 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
      961 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     1021 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     1081 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     1141 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     1201 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
     1261 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
     1321 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
     1381 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
     1441 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
     1501 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
     1561 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
     1621 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
     1681 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
     1741 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
     1801 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
     1861 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
     1921 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
     1981 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
     2041 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
     2101 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
     2161 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
     2221 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
     2281 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     2341 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     2401 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     2461 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     2521 gggggaagat tcttggagag tttttcccgt cggagctcct
//
LOCUS       DAZBTH010000189         2533 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000189 DAZBTH010000000
VERSION     DAZBTH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.195"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..379)
                     /locus_tag="ACHGNW_002809"
     CDS             complement(<1..379)
                     /locus_tag="ACHGNW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN6927055.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            565..1968
                     /locus_tag="ACHGNW_002810"
     CDS             565..1968
                     /locus_tag="ACHGNW_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFN6927056.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1989..2273
                     /locus_tag="ACHGNW_002811"
     CDS             1989..2273
                     /locus_tag="ACHGNW_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFN6927057.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DAZBTH010000190         2505 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000190 DAZBTH010000000
VERSION     DAZBTH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.196"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACHGNW_002812"
     CDS             117..656
                     /locus_tag="ACHGNW_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927058.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1676..>2505
                     /locus_tag="ACHGNW_002813"
     CDS             1676..>2505
                     /locus_tag="ACHGNW_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6927059.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          914 a          334 c          403 g          854 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DAZBTH010000191         2453 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000191 DAZBTH010000000
VERSION     DAZBTH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.197"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1048
                     /gene="sppA"
                     /locus_tag="ACHGNW_002814"
     CDS             14..1048
                     /gene="sppA"
                     /locus_tag="ACHGNW_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN6927060.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            1050..1571
                     /locus_tag="ACHGNW_002815"
     CDS             1050..1571
                     /locus_tag="ACHGNW_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN6927061.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            1568..1954
                     /locus_tag="ACHGNW_002816"
     CDS             1568..1954
                     /locus_tag="ACHGNW_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN6927062.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT          844 a          314 c          480 g          815 t
ORIGIN      
        1 aggaggagtt tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt
       61 gttactatcc attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac
      121 aaccctaaca ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt
      181 agaagaaggc gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga
      241 tacaggtgaa agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca
      301 gctaaagaaa attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc
      361 tgggggagga atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct
      421 agatattcct atctatactg catttaaaaa tacagcggca agtggagggt actacatttc
      481 tgctggttca gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat
      541 tatttctggt ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta
      601 taaaagtggt gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa
      661 taaagttata caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa
      721 aggaagaaat atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg
      781 taaccaagca gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga
      841 ttccctaaaa aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac
      901 aactggcttt gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc
      961 aactacatca gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa
     1021 accaatgtat tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc
     1081 ctaattatgt atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt
     1141 gtattagcat catcacccat gctactatag gtctctttta caaaatcatg ggactggaat
     1201 ctgacaaatc cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt
     1261 ttgtactttt gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa
     1321 gagtagtgag ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta
     1381 tatgtaggtt tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca
     1441 cgcccaagaa acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt
     1501 cattagaagc tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga
     1561 gaattaaatg aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg
     1621 gattttatgt agtcttttag cattgatggg tggtatgttt ctatcttcta ttgattcaac
     1681 tactttaaat actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg
     1741 gaatgttgtt ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg
     1801 ttttttagta agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc
     1861 tattattcca ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat
     1921 tgcgggattg atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa
     1981 cgattaatca attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag
     2041 acatgtagat aaaaaaacta cgtgtctttt atttattcta attttttcga aaaaaagaca
     2101 ctctctgatt gttgtattaa tggaaaaggg gaaagcagca agtaaattta gtttttctat
     2161 taaatattct tcacttttta accttttata gtacttagat ttaatcctat atgtttagag
     2221 agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt agaaacttta
     2281 ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt ggactttgag
     2341 ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt aaatgttttc
     2401 gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat ggg
//
LOCUS       DAZBTH010000192         2436 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000192 DAZBTH010000000
VERSION     DAZBTH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.198"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHGNW_002817"
     CDS             complement(<1..272)
                     /locus_tag="ACHGNW_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927063.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACHGNW_002818"
     CDS             complement(309..446)
                     /locus_tag="ACHGNW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6927064.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACHGNW_002819"
     CDS             complement(448..894)
                     /locus_tag="ACHGNW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927065.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACHGNW_002820"
     CDS             complement(891..1040)
                     /locus_tag="ACHGNW_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927066.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="ACHGNW_002821"
     CDS             complement(1054..>2436)
                     /locus_tag="ACHGNW_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927067.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DAZBTH010000193         2428 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000193 DAZBTH010000000
VERSION     DAZBTH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.200"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1012
                     /locus_tag="ACHGNW_002822"
     CDS             <1..1012
                     /locus_tag="ACHGNW_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFN6927068.1"
                     /translation="QMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELTD
                     DELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQ
                     DVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFC
                     PTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYI
                     KRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSL
                     SKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDI
                     LI"
     gene            1068..1286
                     /locus_tag="ACHGNW_002823"
     CDS             1068..1286
                     /locus_tag="ACHGNW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927069.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1357..2172
                     /locus_tag="ACHGNW_002824"
     CDS             1357..2172
                     /locus_tag="ACHGNW_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927070.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2236..>2428
                     /locus_tag="ACHGNW_002825"
     CDS             2236..>2428
                     /locus_tag="ACHGNW_002825"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6927071.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLM"
BASE COUNT          924 a          277 c          428 g          799 t
ORIGIN      
        1 tcagatgggt gaaattgtta gaggtcctag tcaaatatct attgatgtta catataaatg
       61 taattttaga tgtaaacatt gttacaatgc gagtggtgga gacaattgtt ttatggataa
      121 tgagttgact gatgatgaac tgttagaact tacaaaagat atatgtaatt taaagccata
      181 taatatttgt ttctgtggag gagaaacttt actgagaaaa aaaatagttt tatcttgtgc
      241 cactttatta aaagaggcag gtattcctaa tattgctatt gttactaatg gatcattagt
      301 aactcaggat gttgcaaaag aattattcaa agcaggtatt aatcgatttc aagtaagttt
      361 agatggaaaa aatgctagta gccatgaagc acttagatta tatccaaaca gttttgaaaa
      421 ggcaattaat gctatcaaat tatttagaga attgtccgaa gaagtggatg ttgctttttg
      481 tcctactagt tttaattaca atcaattacg agaagtatat caactagtaa atactcttaa
      541 agtgaagtct ttaagaattc aaccatttat gcttagtggt aggggaatgc aaaataggga
      601 aatagtgcca actgtttctc aatatagaaa actagtttct gatattaact atattaaaag
      661 aaatgaagat tataccaaga tagattgggg agatccaatt gatcatatca tccgacacag
      721 aactttattg aaagattttt cgaattttgt ttctattagg tcagacggag gattaatggc
      781 atcaccttac ttgccaattc aagttggaaa tattaaatgt cacagtcttt ctaaatattg
      841 gcgagcagga ctttataaag ttttttcttt agatttagtt caagatatag ctactcaagt
      901 ttcttcaata caagatatga gtaaagaatt aaaaaaagga catattaaat ggcttaatga
      961 agatatagaa attgatattc tcgatgaata caaacatgat attttaattt aggtttatca
     1021 ttggaaagtt tagataaaca aagtactttt agaaaggagg gaaaaaaatg atttctgaac
     1081 ctcaaatgaa taatatgtat gaaagcccaa ctataatggg agctggtgaa gctgcgccaa
     1141 gagcttgttc agcaattatg gtgtgtgtgt tattagcatc tgcaggagta ggtatccaag
     1201 caggagcggt gtatacttat gctgtagttg ctacagtagc tgtagctgca gccgcagttt
     1261 ggaaaacagt aggattagtt ggttaggaat taataggaga ctaactgtct cctattgatt
     1321 ccaatgatta agctaaggag gaattttaag aaaataatga aatacaataa tttaaaaccg
     1381 aaattactta atgagaacat agaatatata agagaagaaa attatggata tttaaccata
     1441 gttcccaaaa atggaactga attgaaagat aacgttttca atccaacagt aagatatata
     1501 ttggctaact gtactggaaa aaacagtgta aaaaaaatag cgaatggttt aaaaaagaag
     1561 tttcctagtt tagaatatga aaaattctat caagatgttg taggaactat taaatttttc
     1621 aaacactata atattattga tataggagat tatagtatgg aagaaaagaa aatatataaa
     1681 attgaacaaa atttagaatt agaattacta aatgaatcac attatgaaga aatttataat
     1741 tttatcacag atcatcctga aagtatgtat tttcatgaag atatttatca tcaaggaaat
     1801 agtaaagaat tagaagttgt ccttagatat aatttatttt cgtttacgag tgaattttac
     1861 tttttaagac ataaagagag aattgtagga atggtttcta taattgttaa tccatttcaa
     1921 aacttatcct acagtatagt aaattacctc atatgtgagc ctgcatatac tacgactatt
     1981 ttgacaacag tattagaaaa tattaacgaa gtttcaattt tagaagtaaa gaagctagtg
     2041 attcatttgc taaattcaac agaagatgaa tcactaatcc cctatttaaa agattcacat
     2101 ttttttaaag aagcggtact atctaaagaa tatagagagc atgatgtttt gagatacgct
     2161 tacatttatt aaataaaatt tctgatagta ttttaataat tcggatcata tcataagata
     2221 taggagggaa aagaagtgat tttattgatt ttgtctctcg tattactagt tgtatctaat
     2281 ctaatgaaaa ataatcctcc tacaaaaatt aattcttttt atggctttag aactaaaaag
     2341 tcaatgaaaa atgaaaaaag ttggcattta gcacaaaaat tgttggggga tttttcaaaa
     2401 aaaaaagtta tcccagtatt tttgatgg
//
LOCUS       DAZBTH010000194         2408 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000194 DAZBTH010000000
VERSION     DAZBTH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.201"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..464)
                     /locus_tag="ACHGNW_002826"
     CDS             complement(243..464)
                     /locus_tag="ACHGNW_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927072.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ACHGNW_002827"
     CDS             complement(562..807)
                     /locus_tag="ACHGNW_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927073.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ACHGNW_002828"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ACHGNW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="ACHGNW_002829"
     CDS             complement(2031..>2408)
                     /locus_tag="ACHGNW_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN6927074.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DAZBTH010000195         2407 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000195 DAZBTH010000000
VERSION     DAZBTH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.202"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHGNW_002830"
     CDS             5..1150
                     /locus_tag="ACHGNW_002830"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN6927075.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHGNW_002831"
     CDS             1321..1779
                     /locus_tag="ACHGNW_002831"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN6927076.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHGNW_002832"
     CDS             1938..>2407
                     /locus_tag="ACHGNW_002832"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN6927077.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZBTH010000196         2400 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000196 DAZBTH010000000
VERSION     DAZBTH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.203"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHGNW_002833"
     CDS             207..2069
                     /locus_tag="ACHGNW_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6927078.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHGNW_002834"
     CDS             2069..>2400
                     /locus_tag="ACHGNW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6927079.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZBTH010000197         2395 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000197 DAZBTH010000000
VERSION     DAZBTH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.204"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..>2395
                     /gene="esp"
                     /locus_tag="ACHGNW_002835"
     CDS             69..>2395
                     /gene="esp"
                     /locus_tag="ACHGNW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN6927080.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          869 a          375 c          486 g          665 t
ORIGIN      
        1 ataatacgta tcagatttat cttttattct tactaggaaa gtaattatta agggagttga
       61 tttgaataat ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag
      121 tattgaagta cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg
      181 gtcttgtctt gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc
      241 cacttgaaac agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt
      301 ttcaggagaa tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc
      361 aagaactaac tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac
      421 ataatattgc agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag
      481 ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga
      541 cctttgtaag aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata
      601 ttcgtgcaag tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta
      661 atggggtaat tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca
      721 atctcaagac cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata
      781 aaattgtacc gggtattgat agtacgggct actttttaac agattcagat gatttctata
      841 atagcttaga tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa
      901 aatatgagaa acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag
      961 ctataatctt agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa
     1021 agggcacgcc ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg
     1081 gtatacagat gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg
     1141 gaacaaaagt agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag
     1201 gttcaataac taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc
     1261 cagtcgttat tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata
     1321 tgaaagcaac agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt
     1381 cttggttgtc ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt
     1441 atttgcaaga tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga
     1501 atccatatat cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg
     1561 ggaaagtatt catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag
     1621 aaggtactgt attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt
     1681 cagatactgt agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg
     1741 aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta
     1801 ttaaggaatc accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg
     1861 ctaaagaatc aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa
     1921 cagagcctga tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg
     1981 gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa
     2041 atgatgcaac atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac
     2101 ctacatttga taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct
     2161 ataataagta cttcggtaat gcaagtgttg atgcaaacac tggtattgtt acattcactc
     2221 cagctaaagg tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt
     2281 accaagatgg ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg
     2341 aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc gcagg
//
LOCUS       DAZBTH010000198         2393 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000198 DAZBTH010000000
VERSION     DAZBTH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.205"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..496
                     /locus_tag="ACHGNW_002836"
     CDS             104..496
                     /locus_tag="ACHGNW_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927081.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(669..872)
                     /locus_tag="ACHGNW_002837"
                     /pseudo
     CDS             complement(669..872)
                     /locus_tag="ACHGNW_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            838..1035
                     /locus_tag="ACHGNW_002838"
     CDS             838..1035
                     /locus_tag="ACHGNW_002838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927082.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1078..1653)
                     /locus_tag="ACHGNW_002839"
     CDS             complement(1078..1653)
                     /locus_tag="ACHGNW_002839"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6927083.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1808..2334)
                     /locus_tag="ACHGNW_002840"
                     /pseudo
     CDS             complement(1808..2334)
                     /locus_tag="ACHGNW_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          759 a          412 c          376 g          846 t
ORIGIN      
        1 aaatataggt gatttttttt gacttcatat ttataataaa gacacgtaaa tcaattattt
       61 ttcattttac tttaactaat aatgtttatt tggagggatt attatgttag atgatagatt
      121 gacgtgttct aattgtggta cattatttta ctatgaggaa gttagaaata ttgtaaaaca
      181 tagaaaaaaa gaaatgccta tacgttgtcc aaattgcaat gaagttatta agcgaaagtt
      241 gtctcatggt tattttgtca gctacgaact atatgaagaa agtaaaataa atctgaaaaa
      301 aaaaccaaaa tactataaaa atttgaaaac tggagctatt ataactgaaa aagaatttaa
      361 aaatttagtg actgaagaag aaagcaggtt ttataatttg tctggtaata tttgccctgg
      421 atttatggat cagattattg aaaatgagtc aattgaatca attgaaaact atgttcctgt
      481 tgacttacca aaatagaaaa aataatcctg tttttacagt gaattatttt aattttgatt
      541 ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt tctcaatctt
      601 tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
      661 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      721 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      781 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg
      841 aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact
      901 actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta
      961 cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa
     1021 cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca
     1081 atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac
     1141 aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt
     1201 tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa
     1261 tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac
     1321 tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa
     1381 ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa
     1441 ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg
     1501 actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct
     1561 cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt
     1621 cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc
     1681 aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta
     1741 taacatctac tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc
     1801 tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc
     1861 gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt
     1921 agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc
     1981 tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt
     2041 gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg
     2101 cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt
     2161 acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt
     2221 taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt
     2281 caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa
     2341 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttc
//
LOCUS       DAZBTH010000199         2381 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000199 DAZBTH010000000
VERSION     DAZBTH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.206"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..2230
                     /locus_tag="ACHGNW_002841"
     CDS             77..2230
                     /locus_tag="ACHGNW_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN6927084.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          813 a          421 c          530 g          617 t
ORIGIN      
        1 cgcccatttt ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata
       61 ggcggaagga taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt
      121 agaaaaaata agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt
      181 tccttttcgc ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc
      241 tagattgggt tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac
      301 ttctacgatt acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa
      361 agctttagtt acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac
      421 ggctaaagat ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga
      481 tgaaaatctg acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa
      541 agaagccaca aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag
      601 tgcgatggtc aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat
      661 tgctaccatc tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa
      721 ctctggtgtt actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg
      781 agtttcgact ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat
      841 tcttggcagt gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct
      901 agcaaaaggt tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga
      961 agatgtcctg caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt
     1021 ttcgaatcaa aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa
     1081 tgcagaattc caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa
     1141 caacggcaaa gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg
     1201 agaagtgcta gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa
     1261 tgctttaggc acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt
     1321 gacagcaggc tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat
     1381 tcgtctgcaa ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct
     1441 tgatgcagtc aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa
     1501 gatgttagga ctagactata cacctggaat gggactacct agtttagatg aagaagctaa
     1561 agcttatcag caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat
     1621 cgatttgccg aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa
     1681 tgcggataat ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca
     1741 gtttgacaca tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg
     1801 gaaacgagta cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt
     1861 gggtgaagtg aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga
     1921 aaatatggcg atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac
     1981 aacagccaaa ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga
     2041 aacggttgcg gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg
     2101 accaacagac aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca
     2161 agaccacata aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat
     2221 ggcaaaataa aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt
     2281 gtgaaaatag cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt
     2341 gaacaccgtt tactcggcaa aaaaggttgt gacaaaaatt g
//
LOCUS       DAZBTH010000200         2313 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000200 DAZBTH010000000
VERSION     DAZBTH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.207"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..808
                     /locus_tag="ACHGNW_002842"
                     /pseudo
     CDS             1..808
                     /locus_tag="ACHGNW_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          845 a          305 c          465 g          698 t
ORIGIN      
        1 atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt ttacccgagg
       61 aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat gtgattgttg
      121 gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg
      181 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
      241 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
      301 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
      361 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
      421 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
      481 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
      541 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
      601 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
      661 attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat attcctagaa
      721 aatcactgga ttatcgaacg attatcgaac acctttggaa gtatttttga gttacgtaag
      781 tattgatgat ctgtctaatt taatttgaca attaagaaag tattaacttg ttttgtgaaa
      841 aagagaattt gaaaaacgat tggcgaacaa atgctcggta ggtatggtat aaatacagag
      901 ctaattggtg tgatgaaatc agatattcgc gtgagattca gtaaagtagg agttcgaaaa
      961 cagctaggtg acaaacagta ttgctttggt acttcggaat ggtttatcaa attcttatca
     1021 acagttcact tctctttatg tttgagtgat gattcgttag gttattgctt ggaaagaccg
     1081 gatgagagta gaagagttta attttataaa gtgaaaagag gtaatgtacg atatacgtag
     1141 ggctgaacca atagaggata agcaaacgat gttacaggcg caaaattcat agcttactgt
     1201 tttggagata acagaagagg aaataatgca gaagtaaggc gaaaaaagga cggaagccta
     1261 acagcatatg aaataaagaa aaatattata actactgata aatcggtatt gagcaaagta
     1321 ctaataggag tcaaaaacat tcataagcta tgagtgtttc ttggctactt ttttattttt
     1381 atgtatctac aaaagtttcc aggggcagaa cctgtgatta ttaatggaag aacctcaagt
     1441 attgtcttaa atcattgtta tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca
     1501 agttactttt cattacatcg aatttttcag aagttgctta tattggtgat gatattaatg
     1561 atttatcatg tatgacatct gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc
     1621 cacaagaaat gagattgaat tatctgattt tattgtaact cacagtggtg gtgacgaatc
     1681 tgtgatagag tttattgagt ggatattgga atcgatatag tgtagtagta cagaaaaatt
     1741 atcatacaat taatactagg cataagaata ttggcatatt ctagagttaa aagtaactat
     1801 tcgaaaagga taaggttaga attttttgtt ttgccagtgg aagaatataa aaagggcatt
     1861 ggaaattaat atattagaat gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt
     1921 gatgggatgg aaaaatacac ttatttcaaa caacgataat ataattatgt gaaccgaagt
     1981 atactcgctt ctaattcaat cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag
     2041 ttttaatgta tccccatatg atccgaaaaa tggtaattta caattaacag tttgatgatg
     2101 ttgaagatgt ggaaatgctg aaattaccat gatgagaaac acattaatta aagggaatta
     2161 attaactaga atataatctg attcaatttg aattaagcaa aaaaaggctg agttttaaat
     2221 aatcgcgaat tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaaatt
     2281 gtatttccga cgaaagaaaa caaaggagtg taa
//
LOCUS       DAZBTH010000201         2129 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000201 DAZBTH010000000
VERSION     DAZBTH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.208"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(425..1039)
                     /locus_tag="ACHGNW_002843"
     CDS             complement(425..1039)
                     /locus_tag="ACHGNW_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6927085.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1645..2022)
                     /locus_tag="ACHGNW_002844"
     CDS             complement(1645..2022)
                     /locus_tag="ACHGNW_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927086.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          701 a          415 c          323 g          690 t
ORIGIN      
        1 ttctattaga aaaaggtatc ctgttggaca attttgttat atcaggtaaa cttcattgca
       61 ataatgataa caaatgtact ataattactt tgtaaagagt tttggggaag actctttcat
      121 caacgtgtat taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac
      181 tactttttat ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca
      241 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
      301 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
      361 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
      421 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
      481 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
      541 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
      601 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
      661 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
      721 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
      781 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
      841 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
      901 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
      961 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
     1021 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
     1081 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
     1141 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
     1201 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
     1261 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
     1321 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
     1381 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
     1441 agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa
     1501 tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa
     1561 agcttttttc caccaattaa ataaatctct catctcaata gtttatccat tattttatct
     1621 ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag
     1681 agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct
     1741 tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca
     1801 agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc
     1861 ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag
     1921 actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag
     1981 tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt
     2041 tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt
     2101 tctcttatgt aatcattttc ttttataca
//
LOCUS       DAZBTH010000202         2075 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000202 DAZBTH010000000
VERSION     DAZBTH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.209"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1280
                     /locus_tag="ACHGNW_002845"
     CDS             <1..1280
                     /locus_tag="ACHGNW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6927087.1"
                     /translation="LGATSALTIVILILSLFFVIRSSTKKEPIIPEKKITEKAKEPTD
                     DKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQLIDWSDPTQ
                     ISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQK
                     DEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKD
                     KEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKK
                     EIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAPILGKTNIL
                     IYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFYRNARINID
                     GIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1349..1948)
                     /locus_tag="ACHGNW_002846"
     CDS             complement(1349..1948)
                     /locus_tag="ACHGNW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6927088.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          665 a          405 c          352 g          653 t
ORIGIN      
        1 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
       61 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
      121 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
      181 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
      241 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
      301 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
      361 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
      421 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttaaatgat ttgatccaaa
      481 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
      541 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
      601 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
      661 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
      721 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
      781 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
      841 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
      901 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
      961 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     1021 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     1081 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     1141 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     1201 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     1261 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     1321 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     1381 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     1441 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     1501 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     1561 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     1621 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     1681 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     1741 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     1801 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     1861 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     1921 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     1981 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     2041 agctagcaat tccttgttta tgaattcatt gacag
//
LOCUS       DAZBTH010000203         2065 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000203 DAZBTH010000000
VERSION     DAZBTH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.210"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHGNW_002847"
     CDS             <1..1008
                     /locus_tag="ACHGNW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927089.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHGNW_002848"
     CDS             1025..1174
                     /locus_tag="ACHGNW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927090.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHGNW_002849"
     CDS             1171..1617
                     /locus_tag="ACHGNW_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927091.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHGNW_002850"
     CDS             1619..1756
                     /locus_tag="ACHGNW_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6927092.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACHGNW_002851"
     CDS             1794..>2065
                     /locus_tag="ACHGNW_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927093.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAZBTH010000204         2062 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000204 DAZBTH010000000
VERSION     DAZBTH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.211"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..534
                     /locus_tag="ACHGNW_002852"
     CDS             <1..534
                     /locus_tag="ACHGNW_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927094.1"
                     /translation="ISFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSIL
                     TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCS
                     NRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAF
                     SFAREDEDNPKTYDILA"
     gene            complement(595..960)
                     /locus_tag="ACHGNW_002853"
     CDS             complement(595..960)
                     /locus_tag="ACHGNW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN6927095.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1036..1281)
                     /locus_tag="ACHGNW_002854"
     CDS             complement(1036..1281)
                     /locus_tag="ACHGNW_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6927096.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1351..1605)
                     /locus_tag="ACHGNW_002855"
     CDS             complement(1351..1605)
                     /locus_tag="ACHGNW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927097.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1769..>2062)
                     /locus_tag="ACHGNW_002856"
     CDS             complement(1769..>2062)
                     /locus_tag="ACHGNW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927098.1"
                     /translation="AILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMMLS
                     YKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          759 a          353 c          367 g          583 t
ORIGIN      
        1 ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa
       61 agcgttatta ttttagtcta ttttcagggg gggaacctta tgataaaaaa actattttta
      121 ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc aactgaactg
      181 atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga tcacagtgac
      241 tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg taagcatcga
      301 tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga
      361 agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt aaaccacgtc
      421 aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga gtacgctttt
      481 agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc ttgatctaag
      541 atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt tatattaggt
      601 aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa cttcatcgtg
      661 ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga
      721 tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac gtttttccat
      781 ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt gaaaaacata
      841 atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc attcccgatg
      901 ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc ataatcgcat
      961 agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa aaaccagcac
     1021 caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc
     1081 aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg gaatttcttc
     1141 tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag aaacagctac
     1201 ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag tatccagtaa
     1261 aatagcttct tcgatactca tttaatcacc tttcattata gtagaatagt tataatgact
     1321 atagaaagct acacatgcaa tcaatagtac tcaatgtttt ttatttaaac gtatcaaata
     1381 aagaatgacg atcaataaag acaaaaccag tcctgataat tcttctataa aaacactatc
     1441 gggttcaaag tagttgtaat taaaaacttt ccacataaag tagatagccg gaagagtcaa
     1501 aatagctaaa taaaaccgtt ctttgttcca attcataaga taaacaatac ttgcgaccag
     1561 tgcgaccgca ggcacccaaa acaaaactct caccaacgta atcatgtaac ggactcctca
     1621 acgattcata taataaaaga taacatgtta ttgcatacat cacaaatgaa atgctaggaa
     1681 aataaaagaa gaatagatca atgaaaaatg aaaaagcaga acagttttct gctgataagc
     1741 aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga ttgatatcta
     1801 agtaattcaa tacatataaa aagaggacga tcgcattcac taaaaaggaa agaatgatga
     1861 tcccatagcg aatggaaaca tatttaacaa atacttttgt ttgaatacgg gcatatcttt
     1921 ttcctttata gctgagcatc atgacaagaa gcaaaagggt aatcacacct tgaaaaatca
     1981 ttccaataaa cagaattcct gaggtttgat aatttaattg gagaaaataa gctgctgccg
     2041 caactaaatc gaataaaatc gc
//
LOCUS       DAZBTH010000205         2055 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000205 DAZBTH010000000
VERSION     DAZBTH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.212"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHGNW_002857"
     CDS             <1..365
                     /locus_tag="ACHGNW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6927099.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACHGNW_002858"
     CDS             complement(721..1293)
                     /locus_tag="ACHGNW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6927100.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHGNW_002859"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHGNW_002859"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN6927101.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAZBTH010000206         2040 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000206 DAZBTH010000000
VERSION     DAZBTH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.213"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHGNW_002860"
     CDS             <1..1872
                     /locus_tag="ACHGNW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN6927102.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZBTH010000207         2003 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000207 DAZBTH010000000
VERSION     DAZBTH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.214"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHGNW_002861"
     CDS             <1..378
                     /locus_tag="ACHGNW_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN6927103.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHGNW_002862"
     CDS             426..1826
                     /locus_tag="ACHGNW_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN6927104.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZBTH010000208         1993 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000208 DAZBTH010000000
VERSION     DAZBTH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.215"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHGNW_002863"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHGNW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6927105.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHGNW_002864"
     CDS             852..1040
                     /locus_tag="ACHGNW_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6927106.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHGNW_002865"
     CDS             1053..1610
                     /locus_tag="ACHGNW_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6927107.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHGNW_002866"
     CDS             1642..1884
                     /locus_tag="ACHGNW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6927108.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZBTH010000209         1922 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000209 DAZBTH010000000
VERSION     DAZBTH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.217"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..357
                     /locus_tag="ACHGNW_002867"
                     /pseudo
     CDS             8..357
                     /locus_tag="ACHGNW_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(629..919)
                     /locus_tag="ACHGNW_002868"
     CDS             complement(629..919)
                     /locus_tag="ACHGNW_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927109.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1127..1819)
                     /locus_tag="ACHGNW_002869"
     CDS             complement(1127..1819)
                     /locus_tag="ACHGNW_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6927110.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          524 a          367 c          297 g          734 t
ORIGIN      
        1 tacttaattg ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa
       61 ttttcattat atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag
      121 aattcgtcga ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa
      181 gatatagatt cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt
      241 tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc
      301 gagttacata ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac
      361 tagtaatgac tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg
      421 gcttcgaatt gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta
      481 gtgattcatc acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc
      541 gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta
      601 ggacattttt gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt
      661 ttcttgggta tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat
      721 aggatctcac aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc
      781 cttcgtctac ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat
      841 ttaaactgaa acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg
      901 ttccatattt tttctccata accttcacct caataccatt atacgaacat acattccctt
      961 tgtaaagaag aaactctgtt ttatcttttt gactttttgt ctattacctt tcttaaaatc
     1021 atattggcac ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc agcagtatag
     1081 attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat ttacgcaaat
     1141 gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct tttgcttttt
     1201 ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg tctaatttct
     1261 tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt gttgcgattt
     1321 gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt gcttcttgta
     1381 gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg ttgatctcat
     1441 ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg ctgactaatt
     1501 gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct aattcattta
     1561 cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt tctttttgtg
     1621 ctagtcgagc ggtaagatct gtaagcgctt gctctttatc tgtcaatgta tgatcaagct
     1681 gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa ataatttcat
     1741 ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca atcccaccag
     1801 taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca tgctaaaagt
     1861 aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tgtgtaattt taatataaat
     1921 ac
//
LOCUS       DAZBTH010000210         1890 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000210 DAZBTH010000000
VERSION     DAZBTH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.218"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..700
                     /locus_tag="ACHGNW_002870"
     CDS             134..700
                     /locus_tag="ACHGNW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6927111.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            845..932
                     /locus_tag="ACHGNW_002871"
     tRNA            845..932
                     /locus_tag="ACHGNW_002871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:877..879,aa:Ser,seq:cga)
     gene            1266..1832
                     /locus_tag="ACHGNW_002872"
                     /pseudo
     CDS             1266..1832
                     /locus_tag="ACHGNW_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          671 a          282 c          365 g          572 t
ORIGIN      
        1 ttgcaaaaaa aagttaaaga agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa
       61 tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc agtaaagaag
      121 tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa aagctgctta
      181 tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg ctaaaaagat
      241 ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt taaaaaatac
      301 gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg aacgtactgg
      361 tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc atcatagtct
      421 ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg atttttcata
      481 caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat cggatgaata
      541 tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt cattgatgtc
      601 atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac ggacaattga
      661 ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca gtatgaattt
      721 gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa aaagcgattt
      781 ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta aaaatagtac
      841 ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa ccggctaata
      901 ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa
      961 gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct attttttcgt
     1021 ttgattgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaataagggg
     1081 ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac aatatttttt
     1141 gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt tggccttttt
     1201 ttgataaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg
     1261 aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga aggaattttg
     1321 attatgagat gtcttataat gaagtggtca ctattactcc aagaaattat cgtttcgatt
     1381 ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca gctggggtat
     1441 ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg gcaggactga
     1501 attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac gttgcaccat
     1561 ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct aaagaattat
     1621 tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg tcacctttac
     1681 actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa gatggacttc
     1741 atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat tatcaattat
     1801 ttaatctgaa caattatcgc tcgctatcaa atggaacgag agtataaaat attttgtgta
     1861 aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DAZBTH010000211         1833 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000211 DAZBTH010000000
VERSION     DAZBTH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.219"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHGNW_002873"
     CDS             <1..969
                     /locus_tag="ACHGNW_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN6927112.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHGNW_002874"
     tRNA            1159..1232
                     /locus_tag="ACHGNW_002874"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DAZBTH010000212         1800 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000212 DAZBTH010000000
VERSION     DAZBTH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.220"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1382
                     /gene="brnQ"
                     /locus_tag="ACHGNW_002875"
     CDS             30..1382
                     /gene="brnQ"
                     /locus_tag="ACHGNW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6927113.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          310 c          353 g          631 t
ORIGIN      
        1 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
       61 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
      121 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
      181 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
      241 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
      301 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
      361 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
      421 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
      481 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
      541 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
      601 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
      661 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
      721 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
      781 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
      841 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
      901 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
      961 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
     1021 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
     1081 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
     1141 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
     1201 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
     1261 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
     1321 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
     1381 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAZBTH010000213         1768 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000213 DAZBTH010000000
VERSION     DAZBTH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.221"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..290)
                     /locus_tag="ACHGNW_002876"
     CDS             complement(<1..290)
                     /locus_tag="ACHGNW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN6927114.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="ACHGNW_002877"
     CDS             complement(283..624)
                     /locus_tag="ACHGNW_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927115.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="ACHGNW_002878"
     CDS             complement(798..1523)
                     /locus_tag="ACHGNW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN6927116.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
BASE COUNT          474 a          355 c          250 g          689 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatat
//
LOCUS       DAZBTH010000214         1751 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000214 DAZBTH010000000
VERSION     DAZBTH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.222"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..201)
                     /locus_tag="ACHGNW_002879"
     CDS             complement(<1..201)
                     /locus_tag="ACHGNW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6927117.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQT"
     gene            complement(198..851)
                     /locus_tag="ACHGNW_002880"
     CDS             complement(198..851)
                     /locus_tag="ACHGNW_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6927118.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1139..>1751
                     /locus_tag="ACHGNW_002881"
     CDS             1139..>1751
                     /locus_tag="ACHGNW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN6927119.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          557 a          316 c          237 g          641 t
ORIGIN      
        1 agtttgttct atatatttta tttcttttcc attcatatta ctagaaattt catttgccat
       61 attatgaatc ttttcttttc tcgtctccaa atatgtttta ggaaagataa aaaaaattaa
      121 tgaatgaact aatataatta ttattccaag aacagaaaat atttgtatga acatttttgg
      181 aaatatcttt aattttttca ttttctctcc aatttatatc ctacatttct tatagtagtt
      241 atacagtcta attgtaattt ttttctaagt tcttttatat atacatctat tactcgatca
      301 taaggagttt cttcgctatc tttccataca taatcaataa tttgcattct tgttaacact
      361 tgtccattat tatccaataa acattttaat acttctagtt cttttgcatt tacatctatt
      421 tcttcaccat ttattttagc tgtatagcta ttaaaattaa ccgatagatc cttatattca
      481 aacttctcta aatgtccata attcttctta attaaagaat ctattctagc ttttaagacc
      541 ggcaatgaaa atggcttttc tacatatccg tctactaaat tagtaaatgc atcaatttta
      601 tattcttcat cactaaatgc agtcaagatt agaattggta aattgctttt ctttctaatt
      661 tctttcaaca cttctaaacc atttataaaa ggtatctgaa tgtctaagat aaccaaattt
      721 atatcgctat taaattttga caaggcttct cttccatctt tagcttgaat aacagtatat
      781 ccaaattctg aaagatattc acttattccc tctcttatca tattatcatc ttcaacagtt
      841 aatattttca tatatacctc cacttaagcc tatacactag atattataca gcatattatg
      901 gctctataat atctagtgtt ggtgacacct tctgttgcca atactttttc ttttttctaa
      961 taaatgcaaa cagacacctt gaaatccttt agaataaagg gttctataat attttgtgtt
     1021 gttggaaaat cgaaggcgat tttctactaa cactttttct tcgtttcaac tcaaaaagag
     1081 actcactttt ggtaaaattt agtcgaccaa aacatcaccc taccaaagga gtcgctctat
     1141 ggataatcat actagaaaat tactcggatt aacagataaa catttatttt ttgatgaaga
     1201 atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag tttattaaag ggagattgga
     1261 tgactcacct tcccattgtg aacactgtgg atcgattcgt attatccgca acggttccta
     1321 tacgacacgt actcagatat tgaaagtcaa agaaaagttg actattttag aattaaaacg
     1381 tacccgtttt ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga
     1441 tgaacatcat caactaacaa aggaactgaa gcaggcgatt ctcatagaac tgtacgaaaa
     1501 tcaatcacgt aaactaatcg ctaagaagta cttcgtctcg gatgggacgg tgacacggat
     1561 cttgcgtgaa gcaacgaagc attaccagcc acgtatgaac ttccttccga cggtgttgtg
     1621 tatggatgag ttcaaatcaa tgaagtctgt ttctggttcc atgagtttta tttgtgttga
     1681 tgggacaacc catcagctat ttacgatcct tgaagatcgc agactttaca aactaactca
     1741 atacttcctg c
//
LOCUS       DAZBTH010000215         1739 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000215 DAZBTH010000000
VERSION     DAZBTH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.223"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHGNW_002882"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHGNW_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHGNW_002883"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHGNW_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          612 a          253 c          229 g          645 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatata
//
LOCUS       DAZBTH010000216         1732 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000216 DAZBTH010000000
VERSION     DAZBTH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.224"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..458)
                     /locus_tag="ACHGNW_002884"
     CDS             complement(144..458)
                     /locus_tag="ACHGNW_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927120.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            744..983
                     /locus_tag="ACHGNW_002885"
     CDS             744..983
                     /locus_tag="ACHGNW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927121.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1345..1605)
                     /locus_tag="ACHGNW_002886"
     CDS             complement(1345..1605)
                     /locus_tag="ACHGNW_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927122.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          613 a          300 c          229 g          590 t
ORIGIN      
        1 ataaactcta gagaaattaa ttcatcctaa cataaaataa ttgttcactc aaaagttttt
       61 ctagaacaat taaaacagca ggcttcacat tcgctataca tttgtaagaa gtaataattc
      121 ctgtactaaa aggcataatt gctttaatta ttttttgact tctcttcaaa cacaagaagt
      181 agttgtgtaa taattgcaga aataaggatt ccaataatag atataaaatt tgacatggaa
      241 acgtctataa cattaaaaga ataaagtgtt gaagaaagta aactgattga aataacccct
      301 ccgtaaaaaa tagtctgtga atatacccac tttttttcag aagacgtact tcgtttggtt
      361 ctaaaaccta tccctgcatt tctactctta tcaaaaaacc aaatatttag aagtaataat
      421 attgctatta ttaagataaa attactgtaa aaatccaaaa gctatcctcc tagcaactat
      481 ttaaataccc aaaaaaatta cctctagata atatcatacc tacgttttca cttataaatt
      541 ttttcataaa aaaagcgttt agaggcataa tcgttaatac agatggtaac caatgtatct
      601 ggtttgaact gggatatcac cctctccata tatgtattac taagactaat agatactata
      661 aaagacttga tagttttgca ttgattttgc ctaatactga gcataggtgt atgctaaacg
      721 atactataag gagtgtgtac ataatgaaaa gaattctgtt tatcagttta tcaaatttat
      781 tgttacttac tgcttgtggg acttctgcat caaagaacat accttcagat acgcaaattt
      841 cgaatagtat cgattcaaca atttctacaa atgagaaaaa ttctccggta gaaacttccg
      901 atataaaagt ttctacctct gcaaaaaaca gggtaaagaa actgtcagca cgtctccttt
      961 atttcaaaca aactctgaat tagcttcttc atcttcttca gcaaataatg atgatagttt
     1021 cgcgtttact cctaactaaa tgcaaccttt acaaatgctt tacgataaca agcctgagct
     1081 aaatgatcct gatatcagtt ttgcattttt tgaaatgatt ggatcggatg atctttttaa
     1141 aactactagt ttgtctatcc gaaaatagga cagttctggc agtataggtt tttatcgagt
     1201 ttctccacca ggcctcgtta cagagacaga ttctctaggc gaaccttttg aataaatcaa
     1261 aaatcatgaa caatcactgc caactaaata aagtgccttc aaaagcttgg tcttctcgct
     1321 tttgaaggca ctttatttaa atctttatga aactaaagta tgttcatatt tgttgctata
     1381 gttatataga tattctgttg tttccaactc ataaacgtga gtatattttg ccactacttt
     1441 tccgcatttt tctaatattt cattgcgatc gacgtcatga tattcgtcta cgcagatcac
     1501 aacacaattt tctaatttac tgattacttc tccaaccact ttttgtttca agccaatagg
     1561 ttggcaaata tatttttttc ctaaaatgat agtttcgtta ttcatatttc atccatcctt
     1621 aatgtttttt cattattcaa aatgataata ccattaaaat atgattgtga aaatcaataa
     1681 cacttgtgat tttataaaaa aataaaaaaa ccgaaaaaca gaaatgacca ct
//
LOCUS       DAZBTH010000217         1648 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000217 DAZBTH010000000
VERSION     DAZBTH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.225"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACHGNW_002887"
     CDS             138..1604
                     /locus_tag="ACHGNW_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN6927123.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcct
//
LOCUS       DAZBTH010000218         1633 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000218 DAZBTH010000000
VERSION     DAZBTH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.226"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACHGNW_002888"
     CDS             2..400
                     /locus_tag="ACHGNW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6927124.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ACHGNW_002889"
     CDS             384..1079
                     /locus_tag="ACHGNW_002889"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFN6927125.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          616 a          236 c          324 g          457 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttt
//
LOCUS       DAZBTH010000219         1610 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000219 DAZBTH010000000
VERSION     DAZBTH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.227"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHGNW_002890"
     CDS             316..570
                     /locus_tag="ACHGNW_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6927126.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHGNW_002891"
     CDS             571..888
                     /locus_tag="ACHGNW_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6927127.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          567 a          250 c          307 g          486 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa
//
LOCUS       DAZBTH010000220         1586 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000220 DAZBTH010000000
VERSION     DAZBTH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.228"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1473)
                     /locus_tag="ACHGNW_002892"
                     /pseudo
     CDS             complement(33..1473)
                     /locus_tag="ACHGNW_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          524 a          259 c          267 g          536 t
ORIGIN      
        1 attcatcatt actcttattg taagtagttc agtcaaattt gcttgttcaa ttgatcgata
       61 acagtatgaa ttgtttgaaa agtaatcgga tatataattg ttttctcact acctaagcct
      121 tcttggtata aagaactatc gcaaagtata atccgctgag tacaaaagaa agtgtcgtct
      181 actatttctc taagttgttt acaaatgata ttactaatag aaaagtgttc tcccaaaatg
      241 tcttctaact gttgtcgata gtataaatac ctaaagtctg acggagctac tatgaacact
      301 tcaattggag atttccgttt atgctcattt aaaagactta aactaatggt tagtttacga
      361 atagaattct cattccaacg cagaaataaa ttgttttgtt ttcgccattg ttcaagaatt
      421 tcatgaactt ctaagtacaa agcgcgttgc tcttcattta acaagtaagt cttatcaggt
      481 tgaaatatct gaatatcagc ccaaagtgag cggataaacg ggagaagtgc tttatcaaaa
      541 agtatctgat tttcttcaga tatattcaga gataagataa gtttttgact gagctcaatc
      601 accaaagggt tatctgctac atggttttga taaacaacgt tcaagtcttt ttgtaacaat
      661 tcaaagttat cgcatagata atttctagag ttgaatagaa agtagatata aaggcattca
      721 tcttcgtcat gacataaaga gaaagaaagt tgctctaatc ctttctgtat tagctgaaac
      781 aagggagtat gtcgggcaac tcttttttct tcctgactaa aatatattgg gttagtctta
      841 gatctctgca aaccgatagc gatacctaat aaaataaatt gtttttcaac tgtagggtaa
      901 cttcttgaaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct ttgaataatt
      961 gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt tagtaacaaa
     1021 cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta ctgcatttgt
     1081 ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga aatgaaggta
     1141 gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag tacattgaaa
     1201 aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga agagtcgaat
     1261 gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg gagtatgtcg
     1321 gtaatgcatt ccaagtcgtt agtgacagac agtgagtggg caaacgttca attgatctgc
     1381 tatttcgcga atctgaatgg tccctttttc gtgtaattgc tcacaaatgt aaacttgacg
     1441 aaaaatactt ttctccagat aggtttccaa catagtactc ctcctagtca ttttatacat
     1501 aactattata actctaccaa tcggaatagt cctgtaatat attgaatcaa aaatataaat
     1561 tataaaataa ttagatgggg tatttg
//
LOCUS       DAZBTH010000221         1552 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000221 DAZBTH010000000
VERSION     DAZBTH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.229"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHGNW_002893"
     CDS             131..505
                     /locus_tag="ACHGNW_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6927128.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHGNW_002894"
     CDS             complement(619..1248)
                     /locus_tag="ACHGNW_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6927129.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHGNW_002895"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHGNW_002895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          528 a          232 c          270 g          522 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt tt
//
LOCUS       DAZBTH010000222         1538 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000222 DAZBTH010000000
VERSION     DAZBTH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.230"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..326)
                     /locus_tag="ACHGNW_002896"
     CDS             complement(<1..326)
                     /locus_tag="ACHGNW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6927130.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTL"
     gene            complement(622..1494)
                     /gene="scrK"
                     /locus_tag="ACHGNW_002897"
     CDS             complement(622..1494)
                     /gene="scrK"
                     /locus_tag="ACHGNW_002897"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6927131.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          415 a          350 c          302 g          471 t
ORIGIN      
        1 tagagggtac ttactgaaca agagaaggtt cgctctgcac ggccaataat gacgtctggg
       61 gtccaacctt cagttgatct ttttgcaatg tattcttttt cctcagcagg aaaaatgatt
      121 tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagcattc aagtgctgtt
      181 ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg tttaagctgc
      241 ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat tacaagctcg
      301 tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtatgct ttagcgggta
      361 ttacgatttg agtgtaacat aaatggcttt tttatttgtc tcgcttaatt atacaaacgg
      421 cgtgtaaaaa actattgcac aatacaagtg gcctacggct atcgaaattt ccataacttt
      481 cgagcaagaa tttatctcat tcaaggcttg atcttccaag taaaacaaaa accagttaag
      541 cactctgcct aactggttat gactaaattt tcagttgtat ctttctctac caacactaaa
      601 tgtcgaagag ccttttttcg tttatgaatg taagaccgct tctcgagcca atgccaaaca
      661 accgagcgtt ccagcgttgt cttctaatgc tggggtcacg atatatttgc ttaactcagg
      721 tgtttcgaca tacccattga ccaattttgc aaatgcgtcg tggacgtttt gcagcatatg
      781 gcgttgtttc atcacaccac cgccaaaaat gatcacatct ggagaaaaca tcaaggttgt
      841 attgtaggca cattgagcaa tataggaggc ttcgatttcc caataaggat catcttctgc
      901 aacctcttgt ccttttttcc ctaagcgttt ttcgatggcg ggtccagcgg ccatcccttc
      961 taaacagttg ccatgaaatg gacagctgcc agcaaagtca tcttttggat gagggacgac
     1021 gagcatatgc cccatttctg gatgactaaa cccttcgata aagcgccctt cttgtaaggc
     1081 accgccgcca acacccgtac cgatcgtata gtaaacgaca ctgctcaatc ctttgccact
     1141 gccgaagaca tactcaccat aggcagccgc attgacatct gttgtccaag aaatcgggat
     1201 cggaaattct tttttcatgg taccgatgaa atcaaaattt tgccatgcca atttcggggt
     1261 cgaggtaatg tacccataag tggcggaatc gcgatggata tcgataggcc caaatgagcc
     1321 aatcccaatc cctgctaaat ctgcttggta ttgtttgaag aagtcaatga ctaaaggcat
     1381 tgtttcctca ggtgtcgttg tcggaaagct cactctttcc ttgatcgtca tttcttcatc
     1441 gccaatggca cacacaaatt ttgtgccgcc tgcttcaatt gcaccgtata gcatagacat
     1501 ttctcccttt attttagaat gaaatcacta aataaatg
//
LOCUS       DAZBTH010000223         1510 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000223 DAZBTH010000000
VERSION     DAZBTH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.231"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(351..1376)
                     /locus_tag="ACHGNW_002898"
     CDS             complement(351..1376)
                     /locus_tag="ACHGNW_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6927132.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          457 a          247 c          290 g          516 t
ORIGIN      
        1 ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt tattctattt
       61 atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa atgtgtttaa
      121 aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt cttccctgtg
      181 gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac agatggaaaa
      241 taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat tgaaaaaaga
      301 acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt ctacatttct
      361 tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct tttttcttca
      421 gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc taactgattt
      481 gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa gggatcactg
      541 aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc cactttgtaa
      601 atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc tcgtagcttc
      661 attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc tataatccat
      721 tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt gagacgatca
      781 ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg tccattcttg
      841 atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac tgagtagatg
      901 ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattata ttgatccaat
      961 tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg gatcattttt
     1021 tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc cgtttgtaga
     1081 atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac atctgaaaaa
     1141 aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat cgcgtcaaaa
     1201 ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc tttgattgct
     1261 tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa tactctggag
     1321 acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg tttcaatttt
     1381 atatgcacct cctttttggg agcgttttag aataattcca tagtacaata gacattcttt
     1441 tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga ccatattttt
     1501 ttaagaagct
//
LOCUS       DAZBTH010000224         1453 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000224 DAZBTH010000000
VERSION     DAZBTH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.233"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHGNW_002899"
     CDS             complement(<1..625)
                     /locus_tag="ACHGNW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN6927133.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHGNW_002900"
     CDS             complement(784..1050)
                     /locus_tag="ACHGNW_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6927134.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHGNW_002901"
     CDS             complement(1040..1396)
                     /locus_tag="ACHGNW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6927135.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAZBTH010000225         1440 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000225 DAZBTH010000000
VERSION     DAZBTH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.234"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHGNW_002902"
     CDS             105..1406
                     /locus_tag="ACHGNW_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6927136.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZBTH010000226         1417 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000226 DAZBTH010000000
VERSION     DAZBTH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.236"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHGNW_002903"
     CDS             complement(150..1337)
                     /locus_tag="ACHGNW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN6927137.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZBTH010000227         1397 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000227 DAZBTH010000000
VERSION     DAZBTH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.238"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1360
                     /locus_tag="ACHGNW_002904"
     rRNA            <1..1360
                     /locus_tag="ACHGNW_002904"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          384 a          300 c          422 g          291 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaaagaact
     1381 cggaaaaaaa cttatgt
//
LOCUS       DAZBTH010000228         1374 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000228 DAZBTH010000000
VERSION     DAZBTH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.240"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHGNW_002905"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHGNW_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN6927138.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAZBTH010000229         1372 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000229 DAZBTH010000000
VERSION     DAZBTH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.241"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..>1372
                     /locus_tag="ACHGNW_002906"
     CDS             122..>1372
                     /locus_tag="ACHGNW_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6927139.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAZBTH010000230         1364 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000230 DAZBTH010000000
VERSION     DAZBTH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.242"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGNW_002907"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGNW_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN6927140.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZBTH010000231         1339 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000231 DAZBTH010000000
VERSION     DAZBTH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.244"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACHGNW_002908"
     CDS             <1..1309
                     /locus_tag="ACHGNW_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFN6927141.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DAZBTH010000232         1324 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000232 DAZBTH010000000
VERSION     DAZBTH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.245"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..>1324
                     /locus_tag="ACHGNW_002909"
     CDS             42..>1324
                     /locus_tag="ACHGNW_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN6927142.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          451 a          258 c          252 g          363 t
ORIGIN      
        1 aaatcaccac aaaataaaca actgagagga cgatacgcta tatgaatgat tctatcaaaa
       61 aaatgctgag aataacagag aaagatttga tgattacaga ggtctcttac gagacccttc
      121 agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      181 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa
      241 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttatg cgcctaaaaa
      301 agcaacgcta tacctgtaaa aactgtcgca cccattggac ggctcaaagt tattttgtcc
      361 aacccagaca ttcaatcgca aatcatgtta gatataaaat tgcttcttta ctgactgaaa
      421 aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc gttattcgta
      481 cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc cccagagtat
      541 tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc tttatttgcg
      601 cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta cctcgattaa
      661 caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg acagacatga
      721 acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc
      781 ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga
      841 atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag aactggcgct
      901 ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg aaaagtttcc
      961 gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg cttgagtttt
     1021 ctccgcccct aaaggaggcg tatccctttt ttcatgaatt agttgaagcc tttcgagaca
     1081 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacagct
     1141 ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg catcaccaac gcaatgatct
     1201 atccttattc caatggaaaa atagaagcga agaataccca cataaagaca atgaaacgag
     1261 tatcctacgg atttaaatca tttgagaaca tgagaattag aatctttttg atcaatcaat
     1321 taat
//
LOCUS       DAZBTH010000233         1294 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000233 DAZBTH010000000
VERSION     DAZBTH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.246"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHGNW_002910"
     CDS             <1..1244
                     /locus_tag="ACHGNW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN6927143.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZBTH010000234         1287 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000234 DAZBTH010000000
VERSION     DAZBTH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.247"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHGNW_002911"
     CDS             <1..848
                     /locus_tag="ACHGNW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6927144.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAZBTH010000235         1278 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000235 DAZBTH010000000
VERSION     DAZBTH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.248"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..713)
                     /locus_tag="ACHGNW_002912"
     CDS             complement(<1..713)
                     /locus_tag="ACHGNW_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6927145.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKE"
     gene            complement(789..935)
                     /locus_tag="ACHGNW_002913"
     CDS             complement(789..935)
                     /locus_tag="ACHGNW_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFN6927146.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1039..>1278)
                     /locus_tag="ACHGNW_002914"
     CDS             complement(1039..>1278)
                     /locus_tag="ACHGNW_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6927147.1"
                     /translation="SDILEVLQKVYLKLEKQKYELDPGPLINRLVNYLYFTAYTNKIR
                     FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          482 a          201 c          131 g          464 t
ORIGIN      
        1 atttctttta aactcagcaa taaattttta tagtcatgtc taaatctggc aaatttttct
       61 tgatcttttt ccagtgaatc agtatatttt tttagataaa ccaattcttt tcttttcaat
      121 tgctgttcat attgttctcg ttgcctaata gttattctaa taaataaaaa tagaacaaca
      181 aacatttgaa tgattaaaaa tattaagaca ccaaacacaa aatgatcaaa tacttgatat
      241 tgatgagcaa cataagaaac aaataaacca attgtaagta aataaatcat gaatatagca
      301 gtaagtttag aactggactg ttcaacaatc ctatttatag ctaattttct atataaccaa
      361 ataaacatca gcaaaaacaa agaatcaagc attatctgga atactacata accataccct
      421 tttatccctt caaatgaaaa catatacgtc ataaccatgg aagtaataat agatctgaaa
      481 tcatcaataa gcatacataa tagtaattta tttaaaagca ctatattagt tttcttcttt
      541 tcctttaata taaaataact tccgattata ggtatagctg cggaaacatc agtaaataat
      601 tcaacaaaaa aagtgagtgg tatcaataaa catacaaaaa gatattttct tttgttaaga
      661 tcagtattag tcatataaaa taagagaaaa aagaaaaagt ttcccacgaa cattcttaat
      721 agataagctt ttgtatcgat agtaatcaag agatcccctc ctgattttag gatatcataa
      781 aaaaaatttt agttacactt tttaaataat gaaaaaacac attccactaa atttgatgcc
      841 ggttttcctt gaggttttgt tccagcacct cctttaattt ttttaagctc ttgatctgaa
      901 cattgtttag catttagtct atttttttct tccattacga ttccccctac aaactataaa
      961 tacttttatg tcactgattc aatataccta tttttctaat caaaaaattt tgagacatat
     1021 ttaatattat aagtatcatt aaaattgaga tttatctcca taatctgctc gatataaacc
     1081 atttattcca gcagttcttc caatttcact caaatttctt attaattcct cttgatattc
     1141 agtgaatctt attttattag tataagcagt aaaatataga taattcacca atctatttat
     1201 taaaggaccg ggatctaatt catatttttg tttttctaat tttaaatata ccttttgtaa
     1261 aacctcaaga atatcaga
//
LOCUS       DAZBTH010000236         1276 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000236 DAZBTH010000000
VERSION     DAZBTH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.249"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1185
                     /locus_tag="ACHGNW_002915"
                     /pseudo
     CDS             44..1185
                     /locus_tag="ACHGNW_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT          533 a          258 c          224 g          261 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttgg
//
LOCUS       DAZBTH010000237         1267 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000237 DAZBTH010000000
VERSION     DAZBTH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.250"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..>1267
                     /locus_tag="ACHGNW_002916"
     CDS             12..>1267
                     /locus_tag="ACHGNW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6927148.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          423 a          222 c          285 g          337 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagt
//
LOCUS       DAZBTH010000238         1260 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000238 DAZBTH010000000
VERSION     DAZBTH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.251"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..834)
                     /locus_tag="ACHGNW_002917"
     CDS             complement(<1..834)
                     /locus_tag="ACHGNW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFN6927149.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DK"
     gene            complement(840..>1260)
                     /locus_tag="ACHGNW_002918"
     CDS             complement(840..>1260)
                     /locus_tag="ACHGNW_002918"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6927150.1"
                     /translation="GTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLP
                     SLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDIS
                     HRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          385 a          238 c          145 g          492 t
ORIGIN      
        1 tttatcaaat gtctgtgcta tttcatgatt tattctctgt gctaaaacaa tcatataatc
       61 tatcatcaaa taatccacca tcatatcatt atttttccaa taatttaaaa aaaagtctct
      121 aattatagca aacatccatc tagactcttg attacatgct aatgaataac ccgcaaaata
      181 tcctgatgct acggatgcgt gagcataatc aggcctccta atagaccaaa tatcgatatc
      241 aaaatactca tctggtattg gttttgtaca ataaaatgtg gcgtctaacc acaaacctcc
      301 atgttttgct agcaaagata atcttaataa atcagaataa tttgttctcg taatgattcc
      361 tttgtttttc ttttcttcca cccattttgg tatatcaaca tattgtctat aattatcttc
      421 tgttaaaatt ataacagtat gtttccctgc atttcgtttt attgatttta cacaagcttt
      481 tacaatttcc ggcgattgat ccaatccctg ccaccaacaa acccaaatgc agttagaata
      541 tggtgatttt gattgattaa ttatttgatt ttgactataa tcatatgttt tcaaaaaatc
      601 tgaaaaagtc ttttcataat acttcagcat tacctcatgt ttttttaata aatgttcctt
      661 ctgtttttta ctttcattat atccattacg attcatgact tgaatatcaa ttttagcttg
      721 taacgtggca aatgcttcct tccaagatgt tatctttgca attcgtcttg ttgcattcaa
      781 ttcttcggat atgcgatgta taattctttt tatacttgaa tgtttctgtg tcatattctt
      841 taacctcttt gtattaattc aaataactca taatattttg attctaagat tttgtatgct
      901 gtttgaatat tataatctgc ctttattaaa atcgaacgtt ctgaatctcg atgagagata
      961 tctacatttg caatctctct cgcccattct tttgccgatt gatttaaact tatgaaattg
     1021 gtcttatcac tgaaagcaac ttctgtagga actttctccg aaaagaaaca cggtaaatct
     1081 gcaaattgag cttcaatacc cactaacgga accccttcaa ataatgatgg caacattaat
     1141 acgtcaaatg cttgataaaa tttttctata tcatttctat ttcctaaaaa tttaatatca
     1201 tcatctatgc cacttttctt tgcataattt tttatttctt cttccatatc accagttcca
//
LOCUS       DAZBTH010000239         1259 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000239 DAZBTH010000000
VERSION     DAZBTH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.252"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..260)
                     /locus_tag="ACHGNW_002919"
     CDS             complement(<1..260)
                     /locus_tag="ACHGNW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927151.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA"
     gene            complement(277..>1259)
                     /locus_tag="ACHGNW_002920"
     CDS             complement(277..>1259)
                     /locus_tag="ACHGNW_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN6927152.1"
                     /translation="KTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNL
                     TLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          434 a          213 c          143 g          469 t
ORIGIN      
        1 gcatctagct taatcagatt aaataatgtt tgtttaaaat tgctaccatt aatatgactg
       61 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
      121 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
      181 tcagaaattt cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta
      241 cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc
      301 cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga
      361 aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga
      421 tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat
      481 atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc
      541 tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga
      601 aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat
      661 cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat
      721 caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg
      781 tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact
      841 aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc
      901 tccattttta acatattcca gtattttatt ttcataaaga aacagatata tttctggatc
      961 gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac ttagattttc
     1021 tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg
     1081 aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga
     1141 attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa
     1201 aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaa
//
LOCUS       DAZBTH010000240         1246 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000240 DAZBTH010000000
VERSION     DAZBTH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.253"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHGNW_002921"
     CDS             200..742
                     /locus_tag="ACHGNW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927153.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHGNW_002922"
     CDS             898..>1246
                     /locus_tag="ACHGNW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN6927154.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZBTH010000241         1201 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000241 DAZBTH010000000
VERSION     DAZBTH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.254"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..495)
                     /locus_tag="ACHGNW_002923"
     CDS             complement(<1..495)
                     /locus_tag="ACHGNW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6927155.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDT"
     gene            846..1145
                     /locus_tag="ACHGNW_002924"
     CDS             846..1145
                     /locus_tag="ACHGNW_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6927156.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          442 a          171 c          198 g          390 t
ORIGIN      
        1 agtatcaaaa aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga
       61 acagtctaca aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt
      121 tgaaccagat aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata
      181 actttccatc aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat
      241 gctttttgtc aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc
      301 cattctttga aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat
      361 tggatggcag attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc
      421 aaaatcttcg ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac
      481 tttaggaaaa atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa
      541 accaacttac attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc
      601 gctatctctt gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa
      661 taaaaatctt agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat
      721 cgttgccata acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta
      781 gatgtagcgt atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa
      841 tcgtgatgtc taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt
      901 taaatccaga gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag
      961 aaaaaggtca gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag
     1021 caatcagggg agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag
     1081 gaaaatttga aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt
     1141 tataaaaacg agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg
     1201 c
//
LOCUS       DAZBTH010000242         1195 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000242 DAZBTH010000000
VERSION     DAZBTH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.255"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHGNW_002925"
     CDS             <1..1076
                     /locus_tag="ACHGNW_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6927157.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZBTH010000243         1193 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000243 DAZBTH010000000
VERSION     DAZBTH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.256"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1137
                     /locus_tag="ACHGNW_002926"
     CDS             82..1137
                     /locus_tag="ACHGNW_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6927158.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          498 a          110 c          228 g          357 t
ORIGIN      
        1 aagcaaaaag atgataaagt tactttttat gggatgttgg tagaaaacaa attattataa
       61 atattagagg tataaaaagt tatgaaacac aaggtaagtg tgatagtacc aatttacaat
      121 gttgaaaaat atttaagaaa atgtgtggat agtttattga gtcagacatt aaaagagata
      181 gaaatcgtat tagttgatga tggttcgcca gatgcttcgg gggaaatagc agatgagtat
      241 caaaaaaaat attctaatgt aaaaacaata catagagaaa atggggggtt aggaccggca
      301 agaaatacag gaattgagaa tgcgacagga gaatatgtgg cttttcttga ttcagatgat
      361 tgggtagaaa gtgatatgta tgaaaaatta tacttagttg cgtccaaaga aaatgctgat
      421 atagtagtta gtggacattg tgattttgcg aatgacaaag ctatggtgaa aaagcaacat
      481 cctttgagtg ggaatgtata tgatacgcct aatagtatta aaaatgtacg aaaaaattta
      541 tttggacata gtccaaaaga tcaagaagta gaggccttcc cgatgtcagt atgtatgtct
      601 ttgtataata aaaatctgct agataaatat agactaagat ttgaaaaaat tttatctgaa
      661 gatacaattt ttaacttgaa tgcatatgat gtcgcaaata aaattgcttt tacagaatat
      721 acagattatt gttatagaaa agaagaacaa gagtctatta caaaaagttt atctccaaat
      781 aagcagacgg aatttaaaga atttttaata actctaaaag ctatagcatc gaaagaagat
      841 ttagaatgtc aaatcagagc aaaacgtatg gcaattgatt attgtaggct ttatgtgggc
      901 ttagttgatg atgcaaattt gggaattaaa gagaaaagaa agtatataaa aaaatttatt
      961 gaagatgagg gattaattca atgttggaag ggatatccta caaatacgct accatttcaa
     1021 caaaaatttt ttcataaaat gcttgaatgt aagtgttata caatattgtt gttgttaagt
     1081 agaattagaa agataattaa aaatatgacc agaaaaaaat tgtttataat attataaact
     1141 aaaagtatat caaataatag aaattaacaa gttacaaaaa aatattataa aga
//
LOCUS       DAZBTH010000244         1183 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000244 DAZBTH010000000
VERSION     DAZBTH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.257"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1065)
                     /gene="cysK"
                     /locus_tag="ACHGNW_002927"
     CDS             complement(139..1065)
                     /gene="cysK"
                     /locus_tag="ACHGNW_002927"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN6927159.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          320 a          278 c          184 g          401 t
ORIGIN      
        1 atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca aataattttt
       61 ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat aaacctatct
      121 ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat aacgttcacc
      181 attgtcggga accacagcta ataccttctt gccttttcct aattcttttg cgacttttag
      241 cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt ttcgcccaac
      301 ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat catacacaga
      361 agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt gaggtcccgc
      421 ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt cgattttcgg
      481 atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac cgatacctgc
      541 aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac cagtcgtctt
      601 ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt agccgttttc
      661 ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac caggagtcaa
      721 aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca tcgtctcagg
      781 catcacgatg attactttat atccttttgc aactcctacc atcgacaatc cgattcctgt
      841 atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat cgtgttctgc
      901 tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat taaaaaactc
      961 aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt tgacgattgg
     1021 cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca attcctcctt
     1081 gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag tgattcgtct
     1141 ttgactagca aaaaaaagac aaagatatac catatatggt aat
//
LOCUS       DAZBTH010000245         1175 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000245 DAZBTH010000000
VERSION     DAZBTH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.258"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..142)
                     /locus_tag="ACHGNW_002928"
     tRNA            complement(58..142)
                     /locus_tag="ACHGNW_002928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="ACHGNW_002929"
     tRNA            complement(163..233)
                     /locus_tag="ACHGNW_002929"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
     gene            complement(279..350)
                     /locus_tag="ACHGNW_002930"
     tRNA            complement(279..350)
                     /locus_tag="ACHGNW_002930"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Gln,seq:ttg)
     gene            complement(362..434)
                     /locus_tag="ACHGNW_002931"
     tRNA            complement(362..434)
                     /locus_tag="ACHGNW_002931"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:His,seq:gtg)
     gene            complement(437..510)
                     /locus_tag="ACHGNW_002932"
     tRNA            complement(437..510)
                     /locus_tag="ACHGNW_002932"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Trp,seq:cca)
     gene            complement(517..597)
                     /locus_tag="ACHGNW_002933"
     tRNA            complement(517..597)
                     /locus_tag="ACHGNW_002933"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Tyr,seq:gta)
     gene            complement(599..674)
                     /locus_tag="ACHGNW_002934"
     tRNA            complement(599..674)
                     /locus_tag="ACHGNW_002934"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Phe,seq:gaa)
     gene            complement(710..782)
                     /locus_tag="ACHGNW_002935"
     tRNA            complement(710..782)
                     /locus_tag="ACHGNW_002935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Asp,seq:gtc)
     gene            complement(792..864)
                     /locus_tag="ACHGNW_002936"
     tRNA            complement(792..864)
                     /locus_tag="ACHGNW_002936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Val,seq:tac)
     gene            complement(880..951)
                     /locus_tag="ACHGNW_002937"
     tRNA            complement(880..951)
                     /locus_tag="ACHGNW_002937"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Glu,seq:ttc)
     gene            complement(966..1055)
                     /locus_tag="ACHGNW_002938"
     tRNA            complement(966..1055)
                     /locus_tag="ACHGNW_002938"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1019..1021),aa:Ser,seq:gga)
     gene            complement(1095..1168)
                     /locus_tag="ACHGNW_002939"
     tRNA            complement(1095..1168)
                     /locus_tag="ACHGNW_002939"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1132..1134),aa:Asn,seq:gtt)
BASE COUNT          336 a          306 c          265 g          268 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      301 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      361 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      421 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      481 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      541 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      601 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      661 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
      721 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
      781 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
      841 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
      901 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
      961 tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc
     1021 caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt
     1081 tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat taacagtcat
     1141 gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DAZBTH010000246         1151 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000246 DAZBTH010000000
VERSION     DAZBTH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.259"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHGNW_002940"
     CDS             <1..153
                     /locus_tag="ACHGNW_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN6927160.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHGNW_002941"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHGNW_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHGNW_002942"
     CDS             681..944
                     /locus_tag="ACHGNW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6927161.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1151
                     /locus_tag="ACHGNW_002943"
     CDS             944..>1151
                     /locus_tag="ACHGNW_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6927162.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVY"
BASE COUNT          438 a          149 c          231 g          333 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat a
//
LOCUS       DAZBTH010000247         1145 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000247 DAZBTH010000000
VERSION     DAZBTH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.260"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..738)
                     /locus_tag="ACHGNW_002944"
     CDS             complement(364..738)
                     /locus_tag="ACHGNW_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6927163.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(761..>1145)
                     /locus_tag="ACHGNW_002945"
     CDS             complement(761..>1145)
                     /locus_tag="ACHGNW_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFN6927164.1"
                     /translation="FLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFG
                     KTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTA
                     LSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          390 a          215 c          139 g          401 t
ORIGIN      
        1 aataaaaaaa ggcatacaga caataaactg tctatacgcc tcacagactc tattctatac
       61 atctaatttt ggctgtcctg caaaaaatat gtgttagcct ccatcgaaag atcaaacttt
      121 ggaaaacatc attataactt tgcttggtaa gagctttgct aaaacatgat atgacttgta
      181 tacaaaatgt ggagtgtatt ccaataaata ggaaggatta caaaatttga aaccaaataa
      241 gtaaaaaata ctgtcacttt gaattaattt gaaatagtaa attctcatta aactattgaa
      301 atgctgatac caaaaaatga agtacttttc gttcatacca acttagagca cttcgttatc
      361 tatttatttc ttcatttggc ttatctctat aatctcatga tttggtccgt agaaattaaa
      421 gtaacggatt ccattttccc agaacggtaa ttccataata tttttatcta ataattcata
      481 tcctaaatct tgaattgaat tgaatacctc atctatgtca tccgtatcta atgctagatg
      541 atcaatggct cctctacact cagtagtttt atcttcccat atttctatta tcgttccatt
      601 caactcgaaa aagccgactt tacttcccac attcatttct tcattaatca gactaaaccc
      661 taaggattca taaaagattt ttgaagctcc aaaatctttt gttggaattc caatatgttg
      721 taaaccattt aatttcactt acatcatccc ttcaattgtt ttaaaatcca cattcataat
      781 ttattgaata gtctagttta tctaccttga ttgctccctc cgtaacagtc atacttaatg
      841 ctgttccgac tttatcatac attctagtac ttaaagcaac attatcaata tataaaacag
      901 ctatcgtatc atcaacaatt aactttagtt catattcttt gcctatttcc aaaaatagtg
      961 gtcgttgcag ccccttatca aacatttgag gccaaggata gttcggagtt tttccgaaag
     1021 taagctgatt attaatcaat gaaatacgaa attcgtatcc ttcttgagta tcactatcta
     1081 caaataattt taaagcaaac tcctttgtta acgatgtaat agtcaaagtt gtttcaaata
     1141 gaaaa
//
LOCUS       DAZBTH010000248         1143 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000248 DAZBTH010000000
VERSION     DAZBTH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.261"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHGNW_002946"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHGNW_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN6927165.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAZBTH010000249         1136 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000249 DAZBTH010000000
VERSION     DAZBTH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.262"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..462
                     /locus_tag="ACHGNW_002947"
     CDS             52..462
                     /locus_tag="ACHGNW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6927166.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="ACHGNW_002948"
     CDS             476..832
                     /locus_tag="ACHGNW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6927167.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          402 a          164 c          216 g          354 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa tgagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
     1081 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagt
//
LOCUS       DAZBTH010000250         1121 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000250 DAZBTH010000000
VERSION     DAZBTH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.263"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>1121
                     /locus_tag="ACHGNW_002949"
     CDS             14..>1121
                     /locus_tag="ACHGNW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927168.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTR"
BASE COUNT          361 a          152 c          151 g          457 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
     1021 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
     1081 atttatttct ccacagttgg aaaaattttc ttatacaagg a
//
LOCUS       DAZBTH010000251         1105 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000251 DAZBTH010000000
VERSION     DAZBTH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.264"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHGNW_002950"
     CDS             <1..1087
                     /locus_tag="ACHGNW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6927169.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAZBTH010000252         1067 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000252 DAZBTH010000000
VERSION     DAZBTH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.265"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..>1067)
                     /locus_tag="ACHGNW_002951"
     CDS             complement(10..>1067)
                     /locus_tag="ACHGNW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN6927170.1"
                     /translation="VYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFI
                     EHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSS
                     RGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKD
                     FLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIR
                     PTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFI
                     CIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIV
                     FWYYLGSISYLYLKKEN"
BASE COUNT          421 a          180 c          129 g          337 t
ORIGIN      
        1 gtcttcatat taattctcct tttttaaata caagtaagaa atgcttccca aataatacca
       61 gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca tgagcctcca ccatttcatc
      121 tataaaaata gatagtataa aaattaaaac aataccaaga aacgcataat tcactttact
      181 tctaaaaaat gttttgagta aactaaagat atttaacaca tagaaagcat agatacatat
      241 aaaagcaatt ataccacttg atacaagtac ttgaagaaat ccattatgca tgtttccatg
      301 tgctcttttt agttcattca catctgtagt atcaagatct acttttttca aaaattttgc
      361 atcgctattc ccatagaaat cggtctctcc ataaccaaat aacaaagttg gtcttataac
      421 tttaagcgca gctttccaaa tcgtaaaacg accagctgaa atttcggagt tatcactaga
      481 atgctgtaat attacttctt tttctggaag tggagtttta tctgattcat ttgactctat
      541 aacttctact tttctatcaa tcgatacagg aattgttgct aaaaaatcct tagcataatt
      601 agtaccaaaa gaaaaatata ccaatagaac taataatatg cttaccatcc atttgatttt
      661 tacaggtagt acttctctaa tgctctttct aaattttgaa ttggcaaaca acaaaagaaa
      721 taatactaaa aaagaacaaa ttactacctg ggcacctcta gacgaagaaa gtgtaatata
      781 aaatatttgt acgataaaat tcatcatata aaaaacacga atcgtttttg ttatttttga
      841 aattaataca ctaattaaac ttaaaataat agaagtatat ccaaacatag ctccaacatt
      901 tggacttgta tagacaccaa ataaccgatg ttcaataaaa ccttgtcttg caagtaatcc
      961 tggcgttcct gttgggacat gataagaaat caaaaaaata aattgataga tagatattag
     1021 cgcagctaaa aaaattatga taatgaaaac attattaaaa tacacca
//
LOCUS       DAZBTH010000253         1062 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000253 DAZBTH010000000
VERSION     DAZBTH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.267"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHGNW_002952"
     CDS             <1..963
                     /locus_tag="ACHGNW_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6927171.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZBTH010000254         1059 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000254 DAZBTH010000000
VERSION     DAZBTH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.268"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHGNW_002953"
     CDS             complement(37..993)
                     /locus_tag="ACHGNW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927172.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAZBTH010000255         1003 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000255 DAZBTH010000000
VERSION     DAZBTH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.269"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1003
                     /locus_tag="ACHGNW_002954"
                     /pseudo
     CDS             369..1003
                     /locus_tag="ACHGNW_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          296 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ctt
//
LOCUS       DAZBTH010000256          995 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000256 DAZBTH010000000
VERSION     DAZBTH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.270"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHGNW_002955"
     CDS             complement(26..913)
                     /locus_tag="ACHGNW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6927173.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAZBTH010000257          994 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000257 DAZBTH010000000
VERSION     DAZBTH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.271"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACHGNW_002956"
     rRNA            complement(<1..>994)
                     /locus_tag="ACHGNW_002956"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZBTH010000258          990 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000258 DAZBTH010000000
VERSION     DAZBTH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.272"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACHGNW_002957"
     CDS             complement(<1..161)
                     /locus_tag="ACHGNW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN6927174.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
     gene            370..582
                     /locus_tag="ACHGNW_002958"
     CDS             370..582
                     /locus_tag="ACHGNW_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927175.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            744..>990
                     /locus_tag="ACHGNW_002959"
     CDS             744..>990
                     /locus_tag="ACHGNW_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFN6927176.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDIN"
BASE COUNT          351 a          157 c          159 g          323 t
ORIGIN      
        1 tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg atcatataag
       61 atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac ggctacaata
      121 atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc tcctgctatc
      181 tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca gaaccctaaa
      241 aacactatat attccgaaaa gtgagtatct ccattcataa aataatcaac atatttaaaa
      301 aatctaatta gcttttttag tcaatctaaa gttctttatt ctatgatata atgttactgt
      361 agaaagtttg tggcggtttg gcaaatccaa ttgaaggagc gtggtgccta tgggcttatt
      421 agcttatcgg acgtactctg tagaaaggaa atgcctgatt tgttctcatt ggatcaaatc
      481 attgctatta tcggagcagc agcagtagtt attactgccg tagcgaaagc tagcgattcg
      541 attttagctt ggttaaagtt caaaaaagaa caaaaaaaat agagccgtct caacttttgg
      601 cgaggtagac gagctctaaa gctttattta catgccatcc gctttgaacg gactacactg
      661 ggcatgtgac agcatgccct ttcatttata ttatatctca ttcttttata taatgaaagt
      721 aaataaatca tggaggataa aatatggata aatttaatag attaacatta ataaatcagt
      781 ttgaaatttt aaaagcttta aatcctaacg aagaaaaata ctatgctgaa aaaatagaaa
      841 tactaacaga aggttacgaa tatcactata gtgaaatatt tgaaaatatt tctgatccat
      901 tgccagaaga agattcgcgg tttgttctag acatattaaa tatgtataga gatattactt
      961 ttagtaaaaa taaattaaaa gatataaatt
//
LOCUS       DAZBTH010000259          986 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000259 DAZBTH010000000
VERSION     DAZBTH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.273"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHGNW_002960"
     CDS             <1..704
                     /locus_tag="ACHGNW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6927177.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZBTH010000260          944 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000260 DAZBTH010000000
VERSION     DAZBTH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.275"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..884
                     /gene="galU"
                     /locus_tag="ACHGNW_002961"
     CDS             3..884
                     /gene="galU"
                     /locus_tag="ACHGNW_002961"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6927178.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          343 a          158 c          218 g          225 t
ORIGIN      
        1 aagtgaaggt caaaaaagca gtcattcctg ccgctgggct aggaacacgt tttttgccgg
       61 caacaaaagc catggcaaaa gaaatgcttc caatcgtgga taaaccaacg atccagttta
      121 ttgtggaaga agcattggat tcgggaatcg aagacatctt gatcgtaacg ggaaaagaaa
      181 aaagacccat tgaagatcac tttgatgcaa atatcgaatt ggaaaacaat ttgcgggaaa
      241 aggaaaaaac agaattactc gctttagttg aagaaacgac tcaagtcaat cttcacttca
      301 tccgtcaatc tcatccaaga ggactggggg atgccgtgct tcaagcaaaa gcatttatag
      361 gaaatgaacc gtttgtcgtc atgctggggg atgatttgat gaaagatgat atcccattga
      421 ctcaacaatt gatgatggat tatgaagaaa cacaagcttc cgctgttgcc gtcatgcgtg
      481 taccagaaaa tgaaacatct aaatacggaa ttattgatcc taaaacagat agcgaaaagg
      541 gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat gggaaaagca ccaagtaatt
      601 tagccattat cggacgttat ctactgactc ctaaaatatt tgagatatta gaaactcaag
      661 aaccaggagc aggaaacgag atacagctaa cggatgcact tcagactctt aaccagacag
      721 aggcggtcta tgcgagagag tttaaaggaa aaagatacga cgtaggcgat aagttaggat
      781 atatgaagac aaatattgaa tatggacttc aacattcgga aattgggact tcattaagtg
      841 catatattat ccagttgagt aaaaagctaa gaacagaaag ttaactggaa ataagtggcc
      901 tgctgagaat gaaaggctat ttgttattaa agcggcagct tgtg
//
LOCUS       DAZBTH010000261          926 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000261 DAZBTH010000000
VERSION     DAZBTH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.276"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>926
                     /gene="bsh"
                     /locus_tag="ACHGNW_002962"
     CDS             <1..>926
                     /gene="bsh"
                     /locus_tag="ACHGNW_002962"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN6927179.1"
                     /translation="FGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAG
                     IADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEA
                     KKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPS
                     FDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTK
                     LNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTY
                     EDSQITAIDMNKEDLDSHKLISYPIIEKQQIK"
BASE COUNT          328 a          122 c          166 g          310 t
ORIGIN      
        1 attttggaag gaattttgat tatgaaatat cttacaatga agtagttact gttactccaa
       61 gaaattataa gttgaatttt cgaaaggtaa atgatttgga tactcattat gcaatgattg
      121 gtattgccgc tggtatagct gactaccctc tttattacga tgcgacaaat gaaaaaggat
      181 tgagtatggc tgggctaaat ttttctgggt atgctgatta taaagaaata caagaaggga
      241 aagacaatgt atctcctttt gaatttattc cttggatttt aggacaatgc tcaacagtag
      301 gagaagctaa aaaattgtta aaaaatatca atttagcaaa tataaattat agtgacgaac
      361 ttcctttatc ccctttacat tggctattag ctgataaaga aaaatcaatt gtcattgaaa
      421 gtatgaaaga tggacttcat atatatgata accctgtggg cgttcttacc aataatcctt
      481 catttgacta tcaattattt aatttaaaca attatcgtgt cttatcgagt gaaactccta
      541 aaaataattt ttcaaatcaa ataagtttga atgcctatag ccgcggtatg ggagggatag
      601 gcttgcctgg agatttatcc tcagtatctc gttttgttaa agcgactttt acgaagctga
      661 attctgtatc tggagattca gagtcagaaa gtattagtca atttttccat atcttaggtt
      721 cagtagaaca acaaaaaggt ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt
      781 attcttcttg ttgcaatgtt gacaaaggaa tctattatta tcgaacatat gaagacagtc
      841 aaattactgc aattgatatg aataaagaag acttagatag tcataagtta attagttatc
      901 caattataga aaaacaacaa attaaa
//
LOCUS       DAZBTH010000262          904 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000262 DAZBTH010000000
VERSION     DAZBTH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.277"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACHGNW_002963"
     CDS             265..888
                     /locus_tag="ACHGNW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6927180.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZBTH010000263          887 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000263 DAZBTH010000000
VERSION     DAZBTH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.278"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>887
                     /locus_tag="ACHGNW_002964"
     CDS             <1..>887
                     /locus_tag="ACHGNW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFN6927181.1"
                     /translation="IAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLT
                     KRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNR
                     ANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPD
                     LFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIFLINQL"
BASE COUNT          299 a          174 c          168 g          246 t
ORIGIN      
        1 attgcgaaaa attgtcaggt atctttgacc accgttattc gtacgttgaa agagtttaaa
       61 agctacttac caaagcaatc taagaggatt ctccccagag tattgatggt tgatgaattt
      121 cgttcgcacg cttccataga agataaaatg agctttattt gcgcagatgg cgaaacagga
      181 caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat taacaagcta tttcttaggc
      241 tgcgctaatc ctgaagaagt aaaattttta gtcacagaca tgaacgccgc ctacttccag
      301 cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg atcggtttca tattatcaaa
      361 cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa tgaatgaact tcgtaaagta
      421 ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc gctttttgct aaaaaatcgt
      481 gcaaacatca accattatga atacaaaaca tggaaaagtt ttcgagcacc aaaataccca
      541 tttcttacgg aagcgatgat gattgatcga ttgcttggct tttctacgtc cctgaaggag
      601 gcgtatccct attttcatga attagttgaa gcctttcgag acaaagaccc tgacttattt
      661 ttctccttat tggcagaact tcccgaaacg ttagatgacg gctttcggga aaagctccaa
      721 aaccttctga cctatgaaga aggcatcacc aacgcgatga tctatcctta caccaatgga
      781 aaaatagaag cgaagaatac ccatataaag acaatgaaac gagtatccta cggatttaaa
      841 tcatttgaga acatgagaat tagaatcttt ttgatcaatc aattaat
//
LOCUS       DAZBTH010000264          846 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000264 DAZBTH010000000
VERSION     DAZBTH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.279"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..464
                     /locus_tag="ACHGNW_002965"
     CDS             <1..464
                     /locus_tag="ACHGNW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927182.1"
                     /translation="KCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRAR
                     TNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLI
                     LNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            451..>846
                     /locus_tag="ACHGNW_002966"
     CDS             451..>846
                     /locus_tag="ACHGNW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6927183.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFAS"
BASE COUNT          283 a          115 c          122 g          326 t
ORIGIN      
        1 ataaatgtat cttttttgat caacctttat tatctgatgg ttatggcata gatgaaacag
       61 atattttcgc taaagtaaat agtatatttt ctggaaaaaa agaaacttta attaagcttc
      121 atcctagagc aaggacaaat cgttatggtg aaaaaaacat attgaattcc agtttaccgt
      181 ttgaagtctt gctacttgaa aatgacttct cttcaacact tttgattagt cctatatcta
      241 caatttcttt ttcaccatct atgatgtttg aaaagacgat caactctatt atgttggcta
      301 aattaatttt aaatgaatat acagcattat ctgaagataa aaaagaaaca ttgacaaata
      361 ttgttctttt ctgtgaacat tataaccgtc tatcagaaga aaaaatttta ctcccagaaa
      421 actgggcaga attaagaaag ttggtagagt atgggattat ttaacacact ttccaagtca
      481 aaattagtta agtccagttt ctggtatact atagggaatt tttttattaa gggaatttca
      541 ttcataacta ttcctatttt tgtaaatatt actactcaag cagaatatgg tttgattaat
      601 aatttcactt catttgtatc aattttttcg attcttgttg ggctaagcct aaattcggca
      661 attaataatg caagttttga ctataaagat gacataaatg aatttatgtc atcggtactt
      721 tctctagcta cactttcgtt aattgtgttc ttattaatag ggaacatata ttttttgttt
      781 tcaaatgctt tttttgatat gaaccaactt gtatttaatt tgatgttatt acaaagtttt
      841 gctagt
//
LOCUS       DAZBTH010000265          811 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000265 DAZBTH010000000
VERSION     DAZBTH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.280"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHGNW_002967"
     CDS             136..309
                     /locus_tag="ACHGNW_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927184.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          272 a          131 c          102 g          306 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc t
//
LOCUS       DAZBTH010000266          805 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000266 DAZBTH010000000
VERSION     DAZBTH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.281"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>805
                     /locus_tag="ACHGNW_002968"
     CDS             <1..>805
                     /locus_tag="ACHGNW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN6927185.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DAZBTH010000267          774 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000267 DAZBTH010000000
VERSION     DAZBTH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.282"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>774)
                     /locus_tag="ACHGNW_002969"
     CDS             complement(109..>774)
                     /locus_tag="ACHGNW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFN6927186.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          213 a          179 c          150 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccg
//
LOCUS       DAZBTH010000268          761 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000268 DAZBTH010000000
VERSION     DAZBTH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.284"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..521)
                     /locus_tag="ACHGNW_002970"
     CDS             complement(9..521)
                     /locus_tag="ACHGNW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927187.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(528..>761)
                     /locus_tag="ACHGNW_002971"
     CDS             complement(528..>761)
                     /locus_tag="ACHGNW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFN6927188.1"
                     /translation="FNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGT
                     YIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          259 a          110 c           73 g          319 t
ORIGIN      
        1 taacatcctt aatatcttaa tttatccata ttttgagaaa taatactttt ccattcagaa
       61 actaatttag taaggaatct tgcaatgttt tgtctcatta attcattatt tctattatct
      121 ataagatctg ttagatattt ttcaaattct aaaaagctat ccgaattttc ttcgacgatt
      181 aaatacttaa gagaatttat atcggaaaag atagattcaa gtttactaat ctcattttct
      241 aaatctatat tttcacgtgt gattatctca tcactttttc ttaaaaagct aaaaattgca
      301 tcagttgctt tacacttttc cattaaagaa gtatattctt caatttttct tctttcttta
      361 tggtcatttt ttaaatcagt ttcaatgtct tttttcattt gttctatact gagtttgttc
      421 tttttgttct tttgagtaaa tctaaaattc aaacacgaaa aaagtatgtt agcaataaca
      481 cctataatag caaataaatc atttatacta tttaatttca tttatcatca caaacttaga
      541 actttactta tcatctcata agacaagttt ccaaattgat aattattggt agattttaaa
      601 tcattataat tttcgagaaa ttttatataa gtacctctca tcggtttatg gctattaaaa
      661 gtgttaaaat cactatcttc aactatttct tgaaacctat ttaatttttc aataaaaaat
      721 tgtgcataaa gtgctaactt aaattcggta gagctattaa a
//
LOCUS       DAZBTH010000269          752 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000269 DAZBTH010000000
VERSION     DAZBTH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.285"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACHGNW_002972"
     CDS             complement(<1..161)
                     /locus_tag="ACHGNW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6927189.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            374..>752
                     /locus_tag="ACHGNW_002973"
     CDS             374..>752
                     /locus_tag="ACHGNW_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN6927190.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDK
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
BASE COUNT          246 a          126 c          132 g          248 t
ORIGIN      
        1 tattctaagg ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt
       61 gaacgattca agcaatgggc agttttagca acagattgat tcattttgaa ataagactct
      121 attattacaa gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa
      181 ttctttggtc ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa
      241 ttttacaatc ggcgtaataa aaacccgtgg tgctagttaa ttttttccta gaggaaaatg
      301 tctaaacatg ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa ctcccaagaa
      361 atgaggacta catatgcgtt catcaaaata tactgaacat tatacagata cttttataac
      421 attcaaggaa attatgagaa ctgtttctaa catataccac aattgtgtac cagataagat
      481 taaaaaccga agaaatactg ataaactgaa gcaacgcgat acagtgatta ttgcttgtgt
      541 tatatggggc ataattaatg gctatactag ccaaagagcc acgtatagag cggtttgttc
      601 cgtcttattt cctaatggtg actttcctag taggagtcga ttcactcgct taagttcaaa
      661 cttagcttac actatcaaga ttattcgata ctttttcatc aagaagctca ccaaaggtga
      721 actagtcgga attatagata gttttcctag tc
//
LOCUS       DAZBTH010000270          735 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000270 DAZBTH010000000
VERSION     DAZBTH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.286"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>735
                     /locus_tag="ACHGNW_002974"
     CDS             <1..>735
                     /locus_tag="ACHGNW_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6927191.1"
                     /translation="LILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGL
                     VIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGI
                     YSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMV
                     VSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASA
                     IVNILLNMIFIPRLGIIAAGYTTVFT"
BASE COUNT          227 a           94 c          136 g          278 t
ORIGIN      
        1 atttgatttt atcttttata tctacttttt tgaatattgc aatctctgta gctttgatgc
       61 agacattata ttttgatgat aaatatgttg gcagagtagt cggcggaaca ctgtcatttg
      121 tgttgatagg tttagtaatt tatttaagaa taatgataaa gggtgctcat ttttttgata
      181 aaaaacagtg gagatacgct ttaaaaatct ctttgccgct aatcccacat tccttagcaa
      241 atatagtttt agcacaattc gatagaatta tgattaattc ttatttaggt cctgctgcgg
      301 ggggaatata ttcctacatt agtaacatag gtattattct aagcgtaata tggatgtcta
      361 ctaataacgc ctgggttcca tggttctatg gtgagatgag taaaaataat gaaaaaaaag
      421 tgaagaaagt tagtaactat tatttagtag tctttacttt gattacgatg attgtaatgg
      481 tagtttcgat tgatttagca aaatttatgg ctccgaaaga ctattattct gggttatctt
      541 tagtggttcc gataacctta ggctatttct ttcagttttt atatagttta ccagttaatg
      601 ctgaatttta tgagaaaaaa acatcgttta ttgcgattgg tacagtagca agtgctatag
      661 ttaatatact attaaacatg attttcatac ctaggttagg aataattgct gctggataca
      721 caaccgtatt tacgt
//
LOCUS       DAZBTH010000271          733 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000271 DAZBTH010000000
VERSION     DAZBTH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.287"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHGNW_002975"
     rRNA            <1..>733
                     /locus_tag="ACHGNW_002975"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZBTH010000272          723 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000272 DAZBTH010000000
VERSION     DAZBTH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.288"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..232)
                     /locus_tag="ACHGNW_002976"
     CDS             complement(<1..232)
                     /locus_tag="ACHGNW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFN6927192.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMI"
     gene            complement(225..>723)
                     /locus_tag="ACHGNW_002977"
     CDS             complement(225..>723)
                     /locus_tag="ACHGNW_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6927193.1"
                     /translation="FKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTE
                     LIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETT
                     LQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKV
                     GNIDE"
BASE COUNT          255 a          118 c           82 g          268 t
ORIGIN      
        1 taatcattgc tccaatcagt agccaagaaa aacgcatttt aaaaaaattt ttctttataa
       61 aaacctcata caaaactata attccacccc aagcaaaagc tattttcatc gggatattag
      121 ttacattaaa agtactaggc ataaaataaa gcagacaaat gattaaattt attactcgaa
      181 acgcatctcg acttaataaa ctcttctcta aaacaggtac tattctattc atctatattt
      241 ccaactttca aaatttcttt atcaattcta tttaatagtt tatcagagta atccgattta
      301 acttttctta caaataattt ttcactttct tttatttgat ctatatccct ttcatcaaac
      361 catttgttca tggaaggatc aataatatga atatttgcta atttcgaagt ttgtaaagta
      421 gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc ctccaataac atttttattt
      481 ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt catctggggc aaacatacct
      541 attttaaaat tcaggtagtc ttgttgattt tgttcataca tttgtattaa ttctgttagg
      601 cattctttcg acaaagccca ccattgagaa ccatgataca aatcaaattt atggtgtcta
      661 atttttgggg gaagtttaat catcctcaga caacgattaa ttatagctct aaaaatttta
      721 aat
//
LOCUS       DAZBTH010000273          722 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000273 DAZBTH010000000
VERSION     DAZBTH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.289"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            475..>722
                     /locus_tag="ACHGNW_002978"
     CDS             475..>722
                     /locus_tag="ACHGNW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927194.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPN"
BASE COUNT          285 a           84 c          129 g          224 t
ORIGIN      
        1 ctaatactga ttttgtgttt aattgttcat gaagtgaata aacaattgtg aattaaaaaa
       61 ataaaaacct agtataatca ctttaataaa ttgttggtag aaggtgatca aaatgggagt
      121 aaacgaaatg atgaaagtca gattgagttt ttaaatgata ccttcctcag aacttcaaaa
      181 cgaattcaaa gattcgttga caaatcttga gcaagatcat ttaaaaaaat attttctctt
      241 catttgacga aaaatgttta gaaatcttat ataagtgaag ataaagtaat tggtgtaatt
      301 aattgtagag aatagccttt gagcaattga agcgatacaa ttatttagaa aatgaacttc
      361 ctccagcata ctacatgatt ccttacggga cgatgttaaa gaagaaagca agaaatttta
      421 gaagtattag tagataaact aatgggcttt tctcaattta agggagtagc ccaaatgttt
      481 atcaaatatg gtgcagatac agtagcaacc atgtttacag aagaatatca agtagactat
      541 tatgaagtaa tagataaatg tgtacatttt gtttatgaaa aagtgtggaa ttttatgaat
      601 tttgcgataa gtgaagaaat cttacgagta caagaaaaca gaacaggaat atttaccaga
      661 gacagaaagc ttaatttgat tgcgcattat atacggaatc gctggtcatc aataccaaat
      721 tt
//
LOCUS       DAZBTH010000274          712 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000274 DAZBTH010000000
VERSION     DAZBTH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.290"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACHGNW_002979"
     CDS             complement(<1..446)
                     /locus_tag="ACHGNW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6927195.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAZBTH010000275          699 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000275 DAZBTH010000000
VERSION     DAZBTH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.291"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          296 a          105 c          122 g          176 t
ORIGIN      
        1 cttaggcagc ttttgctgtg tagaaaggaa taaataataa cgaaagttca aaagctaatg
       61 ccgactattc tagacattaa agaagacgaa aacgaacgat aaagaaagaa aagttatgaa
      121 taatcgttta ttgaaaaaaa cttgaaaaaa tacaaaaaat tcgttgacca agctaaattt
      181 agttggtata ttattacttg tccaaaagac agaagaaaat atttattcaa aaacataaca
      241 aaaaagttat tgacatgtta ctaacgacat gataagataa ctgagtcgtt aaaaaagaac
      301 cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa
      361 cgataagtta tgataaatga gttgccgaaa gatttcgaaa aacgaaatcg aaaaaaactt
      421 ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat ataaaagttg ctacggcagc
      481 caaaaatgaa tcgggaaaca ctctcgatga agtagacctt tgaaaactga acaaagtaag
      541 acaaaccaaa tgtgtagggc gtcttgattc aattcaagac aacaaacatt tttaacaagc
      601 aagcaatatg ctagcaaaca aattgagctt aacaatcgca agattgttcg aactttttat
      661 gagagtttga tcctggctca ggacgaacgc tggcggcgt
//
LOCUS       DAZBTH010000276          689 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000276 DAZBTH010000000
VERSION     DAZBTH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.292"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..448)
                     /locus_tag="ACHGNW_002980"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="ACHGNW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT          207 a          137 c          105 g          240 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatca
//
LOCUS       DAZBTH010000277          669 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000277 DAZBTH010000000
VERSION     DAZBTH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.293"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..533
                     /gene="spxA"
                     /locus_tag="ACHGNW_002981"
     CDS             135..533
                     /gene="spxA"
                     /locus_tag="ACHGNW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN6927196.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          233 a          100 c          132 g          204 t
ORIGIN      
        1 tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat
       61 agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa
      121 aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg
      181 ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac
      241 cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa
      301 tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt
      361 tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga
      421 ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc
      481 gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata
      541 atacgaaact tggaaaagac tggctctttg tcaaatgatg ttggtaaaga gaaatcttag
      601 aataatttgg acagaacaga aagaagaaaa cctctttctg ttctgtttat ttggttaaaa
      661 atgttttgc
//
LOCUS       DAZBTH010000278          662 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000278 DAZBTH010000000
VERSION     DAZBTH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.294"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..457
                     /locus_tag="ACHGNW_002982"
                     /pseudo
     CDS             122..457
                     /locus_tag="ACHGNW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            553..>662
                     /locus_tag="ACHGNW_002983"
     CDS             553..>662
                     /locus_tag="ACHGNW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6927197.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          169 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtatggat aggctacaat taactagtgg
      481 ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca
      541 ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa
      601 atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta
      661 ac
//
LOCUS       DAZBTH010000279          635 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000279 DAZBTH010000000
VERSION     DAZBTH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.295"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHGNW_002984"
     CDS             complement(<1..270)
                     /locus_tag="ACHGNW_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927198.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHGNW_002985"
     CDS             complement(276..>635)
                     /locus_tag="ACHGNW_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN6927199.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAZBTH010000280          589 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000280 DAZBTH010000000
VERSION     DAZBTH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.296"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..465)
                     /locus_tag="ACHGNW_002986"
     CDS             complement(274..465)
                     /locus_tag="ACHGNW_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927200.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          194 a          117 c           84 g          194 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactaca
//
LOCUS       DAZBTH010000281          526 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000281 DAZBTH010000000
VERSION     DAZBTH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.297"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHGNW_002987"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHGNW_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN6927201.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZBTH010000282          515 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000282 DAZBTH010000000
VERSION     DAZBTH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.299"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>515)
                     /locus_tag="ACHGNW_002988"
     CDS             complement(107..>515)
                     /locus_tag="ACHGNW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN6927202.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DAZBTH010000283          514 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000283 DAZBTH010000000
VERSION     DAZBTH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.300"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..507
                     /locus_tag="ACHGNW_002989"
     CDS             <1..507
                     /locus_tag="ACHGNW_002989"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6927203.1"
                     /translation="DKDGWKMKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLL
                     KKSELKDKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQK
                     CKIKFVPTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQ
                     GEYLYGED"
BASE COUNT          216 a           41 c           98 g          159 t
ORIGIN      
        1 gataaggatg gatggaaaat gaaaaaaact atttttgttt tagcgatttt tatatgtttt
       61 gggctacttt tggtaggcta cttgttgttt aattctaatg aaatgagaac tatttctacg
      121 aatgacttgt taaaaaaaag tgaattaaag gataaaaaaa aagaatctct tgcctttata
      181 atgtttactc aagaagagtg tgagtattgt atagcaataa atccaatcat agatagtgta
      241 aataaggaaa ctttgaataa gataaagaaa tatgatatat ctgtagattt tcaatctaaa
      301 caaaagtgta aaattaagtt tgttccgact attgttgcat atagtaagaa taaagaagtg
      361 gggagaattg acaatgaatt agataatttg tatcgaaaaa agcaagatgg ttcaattgta
      421 atagacaaga aaaaaacaaa gacattgctt atagattgga tgaaaaaaat ggataaacaa
      481 ggagaatatt tgtatggaga agattaaaca gaca
//
LOCUS       DAZBTH010000284          509 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000284 DAZBTH010000000
VERSION     DAZBTH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.301"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHGNW_002990"
     CDS             complement(<1..132)
                     /locus_tag="ACHGNW_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6927204.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAZBTH010000285          496 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000285 DAZBTH010000000
VERSION     DAZBTH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.302"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..339
                     /locus_tag="ACHGNW_002991"
     CDS             <1..339
                     /locus_tag="ACHGNW_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6927205.1"
                     /translation="QASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPS
                     IRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFK
                     EIHDTLESMF"
BASE COUNT          197 a           72 c           68 g          159 t
ORIGIN      
        1 caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga taaatggaaa
       61 aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac cttttataat
      121 ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg ttttaacaaa
      181 caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc tctggagaga
      241 tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt tcataaaggt
      301 tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca gaatgggctt
      361 ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg taaagttata
      421 aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat tcatatgcta
      481 atttatttca ctttat
//
LOCUS       DAZBTH010000286          484 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000286 DAZBTH010000000
VERSION     DAZBTH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.303"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..258)
                     /locus_tag="ACHGNW_002992"
     CDS             complement(61..258)
                     /locus_tag="ACHGNW_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6927206.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DAZBTH010000287          473 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000287 DAZBTH010000000
VERSION     DAZBTH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.305"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          155 a           93 c           56 g          169 t
ORIGIN      
        1 ctacaataac ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat
       61 gaaagatcag gttttattag atctcattct ttcatcactt gaagggattt aatgaactac
      121 tttttcctct ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt
      181 caatgcttga tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta
      241 gaacatattc ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt
      301 tcgataggat aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa
      361 gataggaatc tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca
      421 cgctccacaa aattgtttta tacactacta ttcagcccat tcaacgacca atg
//
LOCUS       DAZBTH010000288          457 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000288 DAZBTH010000000
VERSION     DAZBTH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.306"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>457
                     /locus_tag="ACHGNW_002993"
     CDS             <1..>457
                     /locus_tag="ACHGNW_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6927207.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          185 a           68 c          106 g           98 t
ORIGIN      
        1 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
       61 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAZBTH010000289          456 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000289 DAZBTH010000000
VERSION     DAZBTH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.307"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..327
                     /locus_tag="ACHGNW_002994"
     CDS             124..327
                     /locus_tag="ACHGNW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFN6927208.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..>456
                     /locus_tag="ACHGNW_002995"
     CDS             327..>456
                     /locus_tag="ACHGNW_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6927209.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKK"
BASE COUNT          187 a           46 c           84 g          139 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaa
//
LOCUS       DAZBTH010000290          441 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000290 DAZBTH010000000
VERSION     DAZBTH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.310"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>441
                     /locus_tag="ACHGNW_002996"
     CDS             <1..>441
                     /locus_tag="ACHGNW_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN6927210.1"
                     /translation="SHTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW
                     HYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKK
                     LKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          160 a           80 c           82 g          119 t
ORIGIN      
        1 ttctcatacg acgatttatc gttgggtgca agaatatggc aaactactct atcaaatttg
       61 gaaaaagaaa aataaaaaat ccttttattc atggaaaatg gatgaaacgt acatcaaaat
      121 taaaggaaaa tggcattatt tgtatcgagc catcgatgca gatggtttaa ccttggatat
      181 ttggttacgt aaaaaacggg acacacaagc agcctatgct tttcttaagc ggttagtgaa
      241 gcagtttgat gaaccgaagg ttgtagtcac agataaagcc ccctctatta caagtgcctt
      301 taagaaacta aaagaatacg gcttttatca agggacagaa catcgtacca ttaaatacct
      361 gaataatttg attgaacaag accatcgtcc agtaaagaga cgcaataaat tctatcgaag
      421 tttacgcact gcctctacca c
//
LOCUS       DAZBTH010000291          399 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000291 DAZBTH010000000
VERSION     DAZBTH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.311"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAZBTH010000292          348 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000292 DAZBTH010000000
VERSION     DAZBTH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.313"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>348
                     /locus_tag="ACHGNW_002997"
     CDS             <1..>348
                     /locus_tag="ACHGNW_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6927211.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGF"
BASE COUNT          138 a           64 c           53 g           93 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggtttt
//
LOCUS       DAZBTH010000293          338 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000293 DAZBTH010000000
VERSION     DAZBTH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.314"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACHGNW_002998"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACHGNW_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAZBTH010000294          331 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000294 DAZBTH010000000
VERSION     DAZBTH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.315"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHGNW_002999"
     CDS             <1..>331
                     /locus_tag="ACHGNW_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFN6927212.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAZBTH010000295          321 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000295 DAZBTH010000000
VERSION     DAZBTH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.316"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHGNW_003000"
     CDS             complement(<1..>321)
                     /locus_tag="ACHGNW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6927213.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZBTH010000296          308 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000296 DAZBTH010000000
VERSION     DAZBTH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.317"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHGNW_003001"
     CDS             complement(76..>308)
                     /locus_tag="ACHGNW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6927214.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZBTH010000297          305 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000297 DAZBTH010000000
VERSION     DAZBTH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.318"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGNW_003002"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGNW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN6927215.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZBTH010000298          287 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000298 DAZBTH010000000
VERSION     DAZBTH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.320"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          119 a           41 c           47 g           80 t
ORIGIN      
        1 tattaaacaa cgttgtcgcg cttcattgaa acattttatg aaaattccaa aaaagagttg
       61 acacatgttt ttagacgtga taagataact aagtcgttaa aaagaaccac ttgcgaagct
      121 tgattctatc gaaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg
      181 ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt
      241 tgacaatcgc ttgtctctct gatatgatat aaaagttgct acggcag
//
LOCUS       DAZBTH010000299          283 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000299 DAZBTH010000000
VERSION     DAZBTH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.321"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..283
                     /locus_tag="ACHGNW_003003"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACHGNW_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DAZBTH010000300          282 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000300 DAZBTH010000000
VERSION     DAZBTH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.322"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHGNW_003004"
     CDS             <1..>282
                     /locus_tag="ACHGNW_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN6927216.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZBTH010000301          271 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000301 DAZBTH010000000
VERSION     DAZBTH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.324"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           93 a           47 c           39 g           92 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgttgag agtgtaaaat a
//
LOCUS       DAZBTH010000302          270 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000302 DAZBTH010000000
VERSION     DAZBTH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.325"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHGNW_003005"
     CDS             <1..>270
                     /locus_tag="ACHGNW_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6927217.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAZBTH010000303          261 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000303 DAZBTH010000000
VERSION     DAZBTH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.denovo.326"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACHGNW_003006"
     CDS             <1..>261
                     /locus_tag="ACHGNW_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6927218.1"
                     /translation="LTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGK
                     TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DAZBTH010000304        16909 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.050, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000304 DAZBTH010000000
VERSION     DAZBTH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.050"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHGNW_003007"
     CDS             complement(134..577)
                     /locus_tag="ACHGNW_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN6927219.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHGNW_003008"
     CDS             complement(795..2135)
                     /locus_tag="ACHGNW_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6927220.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHGNW_003009"
     CDS             2404..3300
                     /locus_tag="ACHGNW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN6927221.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHGNW_003010"
     CDS             complement(3450..4391)
                     /locus_tag="ACHGNW_003010"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6927222.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHGNW_003011"
     CDS             4517..5638
                     /locus_tag="ACHGNW_003011"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6927223.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHGNW_003012"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHGNW_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6927224.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHGNW_003013"
     CDS             7465..7992
                     /locus_tag="ACHGNW_003013"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6927225.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACHGNW_003014"
     CDS             8203..9264
                     /locus_tag="ACHGNW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6927226.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACHGNW_003015"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACHGNW_003015"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6927227.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACHGNW_003016"
     CDS             10177..12159
                     /locus_tag="ACHGNW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6927228.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACHGNW_003017"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACHGNW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN6927229.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACHGNW_003018"
     CDS             12881..14305
                     /locus_tag="ACHGNW_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN6927230.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACHGNW_003019"
     CDS             complement(14486..15259)
                     /locus_tag="ACHGNW_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6927231.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACHGNW_003020"
     CDS             complement(15256..16161)
                     /locus_tag="ACHGNW_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6927232.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACHGNW_003021"
     CDS             complement(16315..16635)
                     /locus_tag="ACHGNW_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN6927233.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5455 a         3088 c         3475 g         4891 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacag
//
LOCUS       DAZBTH010000305        13213 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.072, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000305 DAZBTH010000000
VERSION     DAZBTH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.072"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHGNW_003022"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHGNW_003022"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN6927234.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHGNW_003023"
     CDS             complement(2159..3031)
                     /locus_tag="ACHGNW_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6927235.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHGNW_003024"
     CDS             complement(3373..4245)
                     /locus_tag="ACHGNW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6927236.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHGNW_003025"
     CDS             4373..4942
                     /locus_tag="ACHGNW_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN6927237.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHGNW_003026"
     CDS             5009..5746
                     /locus_tag="ACHGNW_003026"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6927238.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHGNW_003027"
     CDS             5977..7245
                     /locus_tag="ACHGNW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6927239.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHGNW_003028"
     CDS             7732..8196
                     /locus_tag="ACHGNW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6927240.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHGNW_003029"
     CDS             8180..8809
                     /locus_tag="ACHGNW_003029"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6927241.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHGNW_003030"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHGNW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6927242.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHGNW_003031"
     CDS             10688..11134
                     /locus_tag="ACHGNW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6927243.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACHGNW_003032"
     CDS             complement(11333..11848)
                     /locus_tag="ACHGNW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6927244.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACHGNW_003033"
     CDS             11965..13167
                     /locus_tag="ACHGNW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6927245.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZBTH010000306         6763 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.122, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000306 DAZBTH010000000
VERSION     DAZBTH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.122"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1545)
                     /gene="efrA"
                     /locus_tag="ACHGNW_003034"
     CDS             complement(<1..1545)
                     /gene="efrA"
                     /locus_tag="ACHGNW_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN6927246.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKK"
     gene            1938..2150
                     /locus_tag="ACHGNW_003035"
     CDS             1938..2150
                     /locus_tag="ACHGNW_003035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN6927247.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            2152..3831
                     /locus_tag="ACHGNW_003036"
     CDS             2152..3831
                     /locus_tag="ACHGNW_003036"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6927248.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(4085..4285)
                     /locus_tag="ACHGNW_003037"
     CDS             complement(4085..4285)
                     /locus_tag="ACHGNW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6927249.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4435..4578)
                     /locus_tag="ACHGNW_003038"
                     /pseudo
     CDS             complement(4435..4578)
                     /locus_tag="ACHGNW_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(4813..5475)
                     /locus_tag="ACHGNW_003039"
     CDS             complement(4813..5475)
                     /locus_tag="ACHGNW_003039"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6927250.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            5867..5926
                     /locus_tag="ACHGNW_003040"
     CDS             5867..5926
                     /locus_tag="ACHGNW_003040"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFN6927251.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            5984..6715
                     /gene="erm(A)"
                     /locus_tag="ACHGNW_003041"
     CDS             5984..6715
                     /gene="erm(A)"
                     /locus_tag="ACHGNW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFN6927252.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         2215 a         1318 c         1150 g         2080 t
ORIGIN      
        1 ttttttcaga cgagcacgta acttcgcatc cgtttgataa tctaatgcag aaaagctgtc
       61 atcaaaaata tagatttccg gacggcgtat gatggctctg gctattgcca atcgttgttt
      121 ttgtccacct gaaaaattgg aaccaccttc tgataaatgt tcattaaatc catctggttt
      181 tcgagaaatg aattctgaag cttgagcaat ctcagctgca cgttccattt cttcttgcgt
      241 agcatcttct ttcccgtatc gcaaattatc tgcaatcgtt ccagtaaata gcagcgcttt
      301 ttgcggaata aacccaatct tatctcgcaa cttgcttagc cgataatctc tcacgtctac
      361 tccatcgatc aagatctttc cttcactcac atcataaaag cgagggatca attggatcaa
      421 cgtagatttt ccgctaccgg tacttccaat aaaagcaacg gtctctcctg gagaggctgt
      481 aaaactgaca tttcgtatca ctggctcttg cgaatggcct ggataagcaa atgttacatt
      541 ttgaaattcg atataccctt tagtttttgt atcagatata ccatttggat cttctacaat
      601 cgaaggtttg gcatctaggg cttcttggat acgtccagct gaaacagctg ctcttggata
      661 catcatgaac acactagcaa ataacatgaa tgagaacaag gcatggaata tatactcgat
      721 aaacgcaatc aaatctccta cacgaagctc gcctgcatcg atttgcactg ccccgctcca
      781 gatgatcaat accattacga tattaaataa gaaataaaaa cccggttgtg caacagccat
      841 caaacggaat aagctttttg aactagaagc ataattctca ttgactacag caaaacgttt
      901 ttcttcaaac ttttcattga cgaatgcacg aatcacacgc aatcctgaaa gattttcccg
      961 caagatactg ttgattttat ctaagttttt ttgttgcttt ttagataacg gttcagacac
     1021 cttagcaatc gctacgactg ccaataatag taaaggtaat gcacctacta catacaaact
     1081 gagtgtcaca cttgtacgca tgaccatgta caaactgaca atgaacatca ttggtgccat
     1141 aaaaccgata cgtaaaatat tttgcaggaa caacatgatc tgataagcat cgtttgttgt
     1201 acgagtgatc aatgaagata cgcctagatt ttcgtattcc tgatgcgaat aactttggat
     1261 cttttcgaac aaatcatctc gaatatctgc tacgatatta gtcgtaatcc tcgaaccaaa
     1321 ataacctaac aagatattca aaataatacc gattacagta ataacgatca taaatatccc
     1381 ttgttgcttc acataatcaa aatcatcatt gcggattcct acatcgatca tacgtgctaa
     1441 aatcgtagga agtcctaatt caatcaaaat aaaaccaaat acgcatatga aattgaaaaa
     1501 caagaatttc ttataacgca tggtataatg ccacattagt ttcactttct catctccttt
     1561 taatcttttg ttacattttc attactaaaa atgatagcac aacgagccta ttaatagaac
     1621 acttctctca taaaaaagtc attatgatta tagcgggatg ataaacattg attttttctt
     1681 tcaatctagt tgatttctat acttatttgc tgttttatgc tagaataaca attaattgtt
     1741 gtatattttt tatacagaag gtacggttga aaaaatatcc gaacagataa gcaaaaacga
     1801 ggcttgtctc tttgactcga atccagtatt ctagtcaaag catatgaaaa accaggtggc
     1861 ttgtcccctc gttttgtgtt gtctaaaaaa atcaactagc taaatcactg ctttacgcag
     1921 ataaaggaga caaaacaatg atctataaag tttattatca agaaacaaaa gtacgcaacc
     1981 ctaaacggga agaaacaaaa tctctttaca ttgaggcaaa aagcgatgta gacgctcgcc
     2041 aacgtgtgga agaaaatacc ccatacaata ttgaattcgt tcaacttcta gaaggaagtc
     2101 atctagagta cgaaaaagaa aatgctgatt tctcattagt ggagttttaa tatgaaactt
     2161 tccatcaaaa ataacgagac aggcgtcttt gctattggtg gattaggtga aatcgggaaa
     2221 aatacgtatg gggtacaatt ccaagatgaa atcattttga ttgatgccgg gatcaaattc
     2281 ccggaagacg atttacttgg aattgactat gtcattcccg actacagcta catcatacaa
     2341 aacttacaca aagtaaaagc cttggtgatt actcacgggc acgaagacca catcggcgga
     2401 ataccctact tgctgaaaca agcaaatatt cctatctatg ctggtcctct cgcattagcg
     2461 ttgatcacaa acaaattaga tgagcatggc ttgcttcgag atgcagagct ccatgagatc
     2521 aatgaagata cgatcatccg ttttagaaag acttctgtca gctttttcag aacgacccat
     2581 agtatcccag atgcattagg gatcgtggtg aaaacacctt ctggaaatat cgtagcgact
     2641 ggagacttca aattcgattt cactcctgtt ggcgaaccag caaacttgca taaaatggct
     2701 cgtatcgggg aagaaggagt cttgtgcctt ttatctgata gtacaaatgc tgagattcca
     2761 acatttacta aatcagaaaa aacgattggg cattctattt tgaagatctt ttcaaaaatc
     2821 gaaggacgta tcatttttgc cagctttgct tctaacatct tccgtttgca gcaagcagca
     2881 gatgccgctg tggcaactgg tcgtaaaatc gctgttttcg gacgaagcat ggaaaatgcc
     2941 atcgtcaacg gtcaacgttt aggatatatc aaagttccag acggcacatt tgtagatgca
     3001 cacgaaatca atcgcttgcc tgccaatgaa acgatgatcc tatgtacagg ttctcaagga
     3061 gaagcgatgg ccgcgttgag ccgcatcgcg aatggtactc accgtcaaat ccaaatccag
     3121 ccagacgata cagtgatctt ttcaagttca cctattcctg gtaatacaac aagtgtcaat
     3181 catttgatca atttgctgtc tgaagctggt gcagaagtga ttcacggaaa aatcaataac
     3241 atccatactt ccggtcacgg tggacaagaa gaacaaaaat taatgcttcg tttgatgaag
     3301 ccaaaatatt ttattcctgt ccacggagaa ttccgcatgt tgaaagtaca tgcttctcta
     3361 gcacaagatg taggtgttcc tgcagaaaac tgcttcatca tggaaaatgg cgatgtttta
     3421 gcgttaacta gcgattctgc gcgtcctgct gggcattttg tggctggaga tgtgtatatc
     3481 gatggtagcg ggatcggtga tattggtaat gtcgttttac gtgaccgtca cctgctttca
     3541 gaagaaggtc ttgtattagc tgtcgcgaca gtggatatca agaaaaaaga aatcttggct
     3601 ggtccagata ttctctctcg tggatttgtc tacatgcgag aatcaggaga aatgatcaat
     3661 gaagctcagc gaatcttatt ccacgcttta agagaatgtt tgaatggacc tgactgttca
     3721 gaatataaat tgaaagaagc gatgactggc gcgcttcagc ctttcttatt cgaacgtaca
     3781 gaaagacatc caatgatttt gccaatggta atgacaccag atgaagaata aataagaggt
     3841 tatgaaatca gcaccttgtc ctgaatataa gagaaaacaa atgaaaaaca tgagaagcca
     3901 tttttgtttg ttcttattcc ttgatacaag gtggctcttt catagcctct taaatttatt
     3961 tactttttca tacaaaaaaa tacctgccac aagagaaatt gtctcttgtg gcaggtattt
     4021 ttgttactcg ctttgttctc tcgtaaaaat tgatgtctac catcaatttg atttacagat
     4081 aaaattaagc ttttacaacg tttgcagctt gtgggccgcg gtctgattct tcaacgtcga
     4141 aagtcactgc ttgaccttct tctaatgttt tgaatccgtc accttggata gctgagaagt
     4201 gaacgaatac gtcgcttcca tcttcacgtg agataaatcc aaatcctttt tcagcgttga
     4261 accattttac tgttccgttt tccataataa tatcctcctc gtgccaaaag cactaattta
     4321 aattgtaaca ttgctcgtgt acgatagagg tgcaacagat gtttcaactt ctattcccaa
     4381 acaaaattac aatataattg tacacttgct tcattttttt tgcaagaaag caacttactt
     4441 ctcttccatc attccttctt ctctcaaact aaaatatcgt ttgcgtccga tgatcaagtg
     4501 gtccagtaca gtgatcccca tcatttctcc acacttttgt atccttttgg taaaattcat
     4561 atcctgctgc gatggaattt catattatca agagtaaatt acaaaagaat catttaaatc
     4621 aatactttca ctttgaatac atgtatttga agtggaaggt acttatttca aaaatagtaa
     4681 aacctgtatc ttaaattact taatagtaac ataagataca ggctgatttt ttattcattg
     4741 ttatttatac taaagcaccc gatagctctg aaaacaatca caaattcaac ttttcaaagc
     4801 cacagcttta agttattttg tccagacaac ccccattgcc caacccattt tatggaattg
     4861 gcatccaggc aacaactttt catataaatc gtaataattt tgttcagata ggtacttatc
     4921 tgaagctaaa tgctcaagcc atgatttaga tgtgttgtga ttataaattc taatcgcatt
     4981 ttttattcca aatttgaaac aatgcggtaa aaactcttgg attgccccta atttataaac
     5041 ataggtaggc ggtgtctcga cttcagatac attatcaaga ataactattc ttccttcctc
     5101 attcaacagt tccttcattt gctgtattac gctggctata tcatccaaat gatgaaaggt
     5161 tgttcggctt acaataaaat caaacttctc attaaaatta agttgttctg cattcatatt
     5221 cagatagacc gtatttgtta gttgacgttt agatttggca agatcgagca tttgattaga
     5281 aatatcaatc cctaccactt catcataata acttgctaat ttctccacta acaaacccga
     5341 gccacatccg atatctaatg ctctgccttt ctttggagac atattagaca caaagaatga
     5401 ataatcattc aaaagctcat tcacgaaatc gtaatcttct gcaaccttat caaactgtga
     5461 ttctattgta ttcaaaaaga tcccccattc ctactttatc gacattcttt cattacttac
     5521 cactttagat gttttttcgt tggggataaa acttcccttt agacaatttt atccaaagac
     5581 aatacaacag tgcaacttta ttaaagtcac tgtcctttat cgcagccttt actttttagt
     5641 aaagacagtg gcttctctta tcaagtttca aaacatatta ttttgaagaa aacgtccatc
     5701 tgaagtgtca agtgcaaaat tacatataaa ggtttattct aaaatgaaaa gatgatacaa
     5761 tcatattcag ttacataagg aggtttcaat tatgtgcacc agtatcgcag tagtagaaat
     5821 tactttatct cattcataat gaaaaaaatg gaaaggagat aaaagtatgg gtactttttc
     5881 tatatttgtt attaataaag ttcgttatca accaaatcaa aattaattgg ttataatgaa
     5941 cgcttaatgt cagttcatta taaccagtaa ggagaaggtt ataatgaacc agaaaaaccc
     6001 taaagacacg caaaatttta ttacttctaa aaagcatgta aaagaaatat tgaatcacac
     6061 gaatatcagt aaacaagaca acgtaataga aatcggatca ggaaaaggac attttaccaa
     6121 agagctagtc aaaatgagtc gatcagttac tgctatagaa attgatggag gcttatgtca
     6181 agtgactaaa gaagcggtaa acccctctga gaatataaaa gtgattcaaa cggatattct
     6241 aaaattttcc ttcccaaaac atataaacta taagatatat ggtaatattc cttataacat
     6301 cagtacggat attgtcaaaa gaattacctt tgaaagtcag gctaaatata gctatcttat
     6361 cgttgagaag ggatttgcga aaagattgca aaatctgcaa cgagctttgg gtttactatt
     6421 aatggtggag atggatataa aaatgctcaa aaaagtacca ccactatatt ttcatcctaa
     6481 gccaagtgta gactctgtat tgattgttct tgaacgacat caaccattga tttcaaagaa
     6541 ggactacaaa aagtatcgat cttttgttta taagtgggta aaccgtgaat atcgtgttct
     6601 tttcactaaa aaccaattcc gacaggcttt gaagcatgca aatgtcacta atattaataa
     6661 actatcgaag gaacaatttc tttctatttt caatagttac aaattgtttc actaaattaa
     6721 agtaataaag cgttctctaa tttcacaaga ggacgcttta ttc
//
LOCUS       DAZBTH010000307         5614 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.142, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000307 DAZBTH010000000
VERSION     DAZBTH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.142"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACHGNW_003042"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACHGNW_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_003043"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN6927253.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ACHGNW_003044"
     CDS             1852..3006
                     /locus_tag="ACHGNW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFN6927254.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ACHGNW_003045"
     CDS             3221..4189
                     /locus_tag="ACHGNW_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN6927255.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ACHGNW_003046"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ACHGNW_003046"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN6927256.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="ACHGNW_003047"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="ACHGNW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DAZBTH010000308         5614 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.143, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000308 DAZBTH010000000
VERSION     DAZBTH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.143"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACHGNW_003048"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACHGNW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_003049"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACHGNW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN6927257.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /gene="vanS"
                     /locus_tag="ACHGNW_003050"
     CDS             1852..3006
                     /gene="vanS"
                     /locus_tag="ACHGNW_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFN6927258.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGAPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ACHGNW_003051"
     CDS             3221..4189
                     /locus_tag="ACHGNW_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN6927259.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ACHGNW_003052"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ACHGNW_003052"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN6927260.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="ACHGNW_003053"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="ACHGNW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1696 a         1092 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg cccctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DAZBTH010000309         3095 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.185, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000309 DAZBTH010000000
VERSION     DAZBTH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.185"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..920
                     /locus_tag="ACHGNW_003054"
     CDS             <1..920
                     /locus_tag="ACHGNW_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6927261.1"
                     /translation="EDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNR
                     SRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDA
                     HHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQF
                     HEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDH
                     STNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFK
                     SADKKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(992..1489)
                     /gene="dfrG"
                     /locus_tag="ACHGNW_003055"
     CDS             complement(992..1489)
                     /gene="dfrG"
                     /locus_tag="ACHGNW_003055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6927262.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            1764..2891
                     /locus_tag="ACHGNW_003056"
                     /pseudo
     CDS             1764..2891
                     /locus_tag="ACHGNW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            2989..>3095
                     /locus_tag="ACHGNW_003057"
     CDS             2989..>3095
                     /locus_tag="ACHGNW_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFN6927263.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPK"
BASE COUNT         1047 a          522 c          516 g         1010 t
ORIGIN      
        1 aagaagacca tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc
       61 cacaagaacc atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga
      121 aaattactaa taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa
      181 ctgctcacta ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg
      241 atgatgactt ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga
      301 gcgatgcaca tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa
      361 aagaattcgg acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa
      421 caaagtttct acggactttg gcatctcatt atctaactga attactaaca agtcatcaat
      481 tccacgaaga ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta
      541 tcaaacatcc attagccact aaggataaag gattttattc agttgattgt agaacggatt
      601 tatctgctct ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga
      661 ccaatagctt tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa
      721 cagctcgtgg tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat
      781 ttgcgattac aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata
      841 aaaaggtttt gacacctgct caaagattag ggattacaga taaacaattt gattggaaag
      901 atataattta tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat
      961 ctttactgtt cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata
     1021 catgaaaata gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct
     1081 cattccattc ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta
     1141 tttttgtgat gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc
     1201 cgaaaataaa aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa
     1261 caatttcaca accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt
     1321 caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct
     1381 ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt
     1441 tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag
     1501 cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat
     1561 cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa
     1621 atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta
     1681 atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt
     1741 gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt
     1801 tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt
     1861 caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc
     1921 aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa
     1981 aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac
     2041 agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat
     2101 attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
     2161 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta
     2221 cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc
     2281 cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg
     2341 gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag
     2401 ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt
     2461 gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc
     2521 ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag
     2581 agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg
     2641 atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta
     2701 ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta acttttttta
     2761 caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac
     2821 ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata
     2881 aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa
     2941 atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac
     3001 aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact
     3061 atacttaaaa atttcccttt atactgcccc aaatg
//
LOCUS       DAZBTH010000310         2429 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.199, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000310 DAZBTH010000000
VERSION     DAZBTH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.199"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1161)
                     /locus_tag="ACHGNW_003058"
     CDS             complement(58..1161)
                     /locus_tag="ACHGNW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6927264.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1714..2262
                     /gene="aac(6')"
                     /locus_tag="ACHGNW_003059"
     CDS             1714..2262
                     /gene="aac(6')"
                     /locus_tag="ACHGNW_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6927265.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          769 a          427 c          478 g          755 t
ORIGIN      
        1 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
       61 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
      121 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
      181 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
      241 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
      301 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
      361 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
      421 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
      481 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
      541 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
      601 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
      661 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
      721 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
      781 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
      841 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
      901 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
      961 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
     1021 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
     1081 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
     1141 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
     1201 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
     1261 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
     1321 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
     1381 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
     1441 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
     1501 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
     1561 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
     1621 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
     1681 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
     1741 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
     1801 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
     1861 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
     1921 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
     1981 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
     2041 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
     2101 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
     2161 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
     2221 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
     2281 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
     2341 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     2401 aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DAZBTH010000311         1984 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000311 DAZBTH010000000
VERSION     DAZBTH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.216"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..388
                     /locus_tag="ACHGNW_003060"
                     /pseudo
     CDS             39..388
                     /locus_tag="ACHGNW_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(660..950)
                     /locus_tag="ACHGNW_003061"
     CDS             complement(660..950)
                     /locus_tag="ACHGNW_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6927266.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1158..1850)
                     /locus_tag="ACHGNW_003062"
     CDS             complement(1158..1850)
                     /locus_tag="ACHGNW_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6927267.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          547 a          377 c          299 g          761 t
ORIGIN      
        1 cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg
       61 caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt
      121 agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata
      181 caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca
      241 aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat
      301 ttacttttac gactatgata atcacctctt cgagttacat accggtactc tccaggaacg
      361 tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct
      421 ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag
      481 caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg
      541 tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg
      601 tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc
      661 tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct
      721 accacaattt tgggtttccc taacacatga taggatctca caacaaattg ctttctatta
      781 ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa
      841 ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca
      901 ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc
      961 tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt
     1021 tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga
     1081 catgtactaa aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt
     1141 ttacttgtta agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca
     1201 gctgtatctt ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt
     1261 gagttttcag cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca
     1321 tttgtttttt cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt
     1381 aattctgaaa gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga
     1441 ttgatttcat catttttttg gttgatctca tctagtttga cattgatttc attttgtttg
     1501 ctttgtagtt gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc
     1561 gattgatttt tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc
     1621 tttgaatctt gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaagcgct
     1681 tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata
     1741 tctgtctctc caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt
     1801 agacatagaa atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct
     1861 ccttaatcca atacttttac atgctaaaag taagtgatct caaacacaat gtcaagcgtt
     1921 tttaaattac atgtgtaatt ttaatataaa tactatcaaa tactcattta atcctccttt
     1981 ttta
//
LOCUS       DAZBTH010000312         1504 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.232, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000312 DAZBTH010000000
VERSION     DAZBTH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.232"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHGNW_003063"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHGNW_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHGNW_003064"
     CDS             394..477
                     /locus_tag="ACHGNW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN6927268.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHGNW_003065"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHGNW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6927269.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZBTH010000313         1421 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000313 DAZBTH010000000
VERSION     DAZBTH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.235"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACHGNW_003066"
     CDS             <1..1350
                     /locus_tag="ACHGNW_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFN6927270.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DAZBTH010000314         1408 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000314 DAZBTH010000000
VERSION     DAZBTH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.237"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACHGNW_003067"
     CDS             <1..372
                     /locus_tag="ACHGNW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFN6927271.1"
                     /translation="KQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNSIA
                     KEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVMELE
                     KENKKLQNQIQKLYGDLYNKE"
     gene            526..1308
                     /locus_tag="ACHGNW_003068"
     CDS             526..1308
                     /locus_tag="ACHGNW_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFN6927272.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          531 a          194 c          277 g          406 t
ORIGIN      
        1 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
       61 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
      121 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
      181 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
      241 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
      301 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
      361 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
      421 gtcagcttta ttgaacagtc atttaggtat atgtccaatc tagggtaagt aaattgagta
      481 tcaatataaa ctttatatga acataatcag ttacgaggtg aaatcatgag caatttgatt
      541 aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga
      601 agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac
      661 agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa
      721 ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc tgttagacca
      781 cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga
      841 gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca
      901 agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta
      961 tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca
     1021 attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt
     1081 ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc
     1141 gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact ggatatagct
     1201 agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa
     1261 gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt
     1321 gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt
     1381 gggaagaata aagcgtcctc ttgtgaaa
//
LOCUS       DAZBTH010000315         1350 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5386
            SAMEA115707688-rid22985063.guided.243, whole genome shotgun
            sequence.
ACCESSION   DAZBTH010000315 DAZBTH010000000
VERSION     DAZBTH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707688
            Sequence Read Archive: ERR13197416
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337962.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:02:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,913
            CDSs (with protein)               :: 2,913
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 53 of 97
            Pseudo Genes (internal stop)      :: 25 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707688-rid22985063.guided.243"
                     /isolate="VRE5386"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHGNW_003069"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHGNW_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHGNW_003070"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHGNW_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN6927273.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
